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2019

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Deep Fakes: A Looming Challenge For Privacy, Democracy, And National Security, Robert Chesney, Danielle K. Citron Dec 2019

Deep Fakes: A Looming Challenge For Privacy, Democracy, And National Security, Robert Chesney, Danielle K. Citron

Faculty Scholarship

Harmful lies are nothing new. But the ability to distort reality has taken an exponential leap forward with “deep fake” technology. This capability makes it possible to create audio and video of real people saying and doing things they never said or did. Machine learning techniques are escalating the technology’s sophistication, making deep fakes ever more realistic and increasingly resistant to detection. Deep-fake technology has characteristics that enable rapid and widespread diffusion, putting it into the hands of both sophisticated and unsophisticated actors. While deep-fake technology will bring with it certain benefits, it also will introduce many harms. The marketplace …


Prophetic Patents, Janet Freilich Dec 2019

Prophetic Patents, Janet Freilich

Faculty Scholarship

In most contexts, making up data is forbidden - considered fraudulent or even immoral. Not so in patents. Patents often contain experimental data and it is perfectly acceptable for these experiments to be entirely fictional. These so-called "prophetic examples" are not only explicitly permitted by both the Patent and Trademark Office and federal courts, but are considered almost equivalent to factual data in patent doctrine. Though prophetic examples are thought to be common, there are no studies of these experiments, no explanation for why fictional data are allowed in patents, and no evaluation of the practice.

Here, I provide the …


The Unnecessary And Unfortunate Focus On “Animus,” “Bare Desire To Harm,” And “Bigotry” In Analyzing Opposition To Gay And Lesbian Rights, James E. Fleming Dec 2019

The Unnecessary And Unfortunate Focus On “Animus,” “Bare Desire To Harm,” And “Bigotry” In Analyzing Opposition To Gay And Lesbian Rights, James E. Fleming

Faculty Scholarship

I am delighted to participate in this symposium on Professor Linda C. McClain’s wonderful new book, Who’s the Bigot? Learning from Conflicts over Marriage and Civil Rights Law. All of the other papers in this symposium focus on Masterpiece Cakeshop, Ltd. v. Colorado Civil Rights Commission (and thus connect with Chapter Eight of her book, on claims of religious exemptions from protections of gay and lesbian rights), while my piece will join issue with the related Chapter Seven, on bigotry, motives, and morality in the Supreme Court’s gay and lesbian rights cases. In this brief Essay, I cannot do justice …


The Relationships Between School Burnout And Perceived Teacher Support In High School Male Students, Travis S. Moots Dec 2019

The Relationships Between School Burnout And Perceived Teacher Support In High School Male Students, Travis S. Moots

Doctoral Dissertations and Projects

The purpose of this quantitative correlational research study is to determine if perceived teacher support predicts school burnout in male high school students. High school males from four private high schools in eastern North Carolina and southeast Virginia participated in the study. The predictor variables were overall perceived teacher support, as well as four subcategories (i.e., invested, positive regard, expectations, and accessible) of perceived teacher support. Data was collected by utilizing student surveys to measure each of the variables. The Teacher Support Scale measured perceived teacher support, and the School Burnout Inventory measured school burnout among the high school males. …


A Causal-Comparative Study Of Burnout Among Rural Elementary, Middle, And High School Teachers, Keisha N. Hamby Dec 2019

A Causal-Comparative Study Of Burnout Among Rural Elementary, Middle, And High School Teachers, Keisha N. Hamby

Doctoral Dissertations and Projects

There is a prevalent shortage of school teachers in the United States. Teacher burnout is a chronic issue that plagues school districts. Burnout is one of the primary causes of teachers leaving the profession altogether. The purpose of this study was to determine differences of burnout among elementary, middle, and high school teachers in a rural area. A gap in literature was addressed by comparing the burnout levels of teachers in a rural school district across all grade levels. The chosen research design for this study was a causal-comparative design. The independent variable was teachers’ grade across three levels (elementary, …


Compassionate Divorce Recovery For Christian Women, Alice Drusilla Hoag Dec 2019

Compassionate Divorce Recovery For Christian Women, Alice Drusilla Hoag

Doctoral Dissertations and Projects

Depression, anxiety, anger, and guilt causes significant distress for 70% of divorcees, typically lasting upwards of two years, while 15-30% report more devastating and life-altering distress for the rest of their lives. Christians divorcees have the added shame of spiritual failure, leading to an increased sense of judgment and isolation from their worship communities. Self-compassion is an attitude toward oneself comprised of self-kindness, common humanity, and mindfulness, and has been shown to reduce depression, anxiety, shame, stress, and social isolation. To date, there are no published divorce recovery programs specifically tailored for Christians utilizing self-compassion techniques. This quasi-experimental study analyzed …


The Intersection Of Ministry And Mental Health: Worship Leaders With Depression, Benjamin Bartow Harrison Dec 2019

The Intersection Of Ministry And Mental Health: Worship Leaders With Depression, Benjamin Bartow Harrison

Doctoral Dissertations and Projects

Despite the fact the mental health issues are becoming more destigmatized among those in society, pastors and other church staff still face unique challenges when seeking treatment for mental health issues. This study considers the perspectives of ministers who often struggle in silence with depression. This qualitative historical research study will identify the unique challenges faced when battling depression that have not yet been studied concerning the lives of worship leaders in various stages of such mental health issues. Time demands, financial challenges, and stigma have emerged as common themes through exploration of a small body of existing literature and …


Sustainable African American Marriages: Evaluating The Impact Of Closeness To God And Religiosity On Marital Satisfaction And Longevity, Kimberly Anissa Gregory Dec 2019

Sustainable African American Marriages: Evaluating The Impact Of Closeness To God And Religiosity On Marital Satisfaction And Longevity, Kimberly Anissa Gregory

Doctoral Dissertations and Projects

Religion and religious involvement extend beyond individual satisfaction to also include influencing marital and family relationships. In spite of the research supporting the perceived benefits of marriage, including overall emotional and physical health, decrease in stress, alcohol and drug use, and increase in lifespan, African Americans marriage rates are lower compared to White Americans. Consequently, African Americans have the highest divorce rate and the highest incidences of single-parent homes. Religiosity is often overlooked as an important component that contributes to satisfaction and enduring relationships. There is also a perceived relationship between closeness to God and romantic attachment that leads to …


The Use Of Patient Diaries In The Cvicu To Prevent Post-Intensive Care Syndrome And Improve Family Satisfaction With Care, Denise M. Goodberlet Dec 2019

The Use Of Patient Diaries In The Cvicu To Prevent Post-Intensive Care Syndrome And Improve Family Satisfaction With Care, Denise M. Goodberlet

Doctoral Dissertations and Projects

Patients in the ICU are at risk for adverse physiological and psychological symptoms including anxiety, depression and confusion common with post intensive care syndrome. The presence of these symptoms is correlated with poor satisfaction with care and decreased quality of life. This creates stress for patients as well as the families of these patients. This evidence-based practice (EBP) project sought to answer the clinical question: For patients and families in the cardiovascular intensive care unit (CVICU), can participating in an intensive care diary program, when compared to standard of care, decrease the symptoms and incidence of post-intensive care syndrome in …


Eradicating Recidivism: Evangelism For African American Men Impacted By Mass Incarceration, Anthony Mark Berry Dec 2019

Eradicating Recidivism: Evangelism For African American Men Impacted By Mass Incarceration, Anthony Mark Berry

Doctoral Dissertations and Projects

The Prison Industrial Complex impacts 2.3 million people, affects the poor, and the nation's minority family's ability to retain a sense of unity. These data impact community health with the absence of the father figures, lack of power to gain viable income, and in some cases, the right to vote. The issue decimates African American young men. This topic is needed because the research suggests a cyclic nature of excavation of human capital by incarceration and is depleting communities of capital by jailing wage earners from the Black community. Voting capital is dwindling in some states where felony crimes remove …


The American Unreformation: The Undoing Of American Evangelical Prominence And The Rise Of The Religiously Unaffiliated Nones, H G. Walker Dec 2019

The American Unreformation: The Undoing Of American Evangelical Prominence And The Rise Of The Religiously Unaffiliated Nones, H G. Walker

Doctoral Dissertations and Projects

With over sixty percent of Protestant churches in America experiencing either plateaued or declining numbers, the ineffectiveness or outright inattention to evangelistic efforts is magnified by the aggressive growth of the Nones population; therefore, this dissertation project seeks to answer one central question related to the current increase in the number of Americans identifying as religiously unaffiliated: Are there identifiable religious resurgence mechanisms in American religious history that address the current rise of the Nones and if initiated would facilitate future religious resurgence? Lower levels of religious affiliation reveal a weakening of the heart of the Evangelical position on evangelization …


Effective Design Features For The Management Of Behavioral Health Patients In General Emergency Departments Of Hospitals, Rheena Ware Luchansky Dec 2019

Effective Design Features For The Management Of Behavioral Health Patients In General Emergency Departments Of Hospitals, Rheena Ware Luchansky

Electronic Theses and Dissertations

This study investigated nursing staff members perspectives of their existing Emergency Department (ED) and their ability to care appropriately for behavioral health patients within the environment. The study involved three rural hospitals in eastern Texas that may not always have the proper resources to care effectively for this vulnerable patient population. The researcher administered a paper-based survey utilizing a Likert-scale response system to nursing staff across all facilities and received participation from 56 respondents. Survey questions were designed to investigate the current ED environment and identify design features available to assist with caring for behavioral health patients. Data gathered revealed …


An Analysis Of The Radiometric Quality Of Small Unmanned Aircraft System Imagery, Baabak G. Mamaghani Dec 2019

An Analysis Of The Radiometric Quality Of Small Unmanned Aircraft System Imagery, Baabak G. Mamaghani

Theses

In recent years, significant advancements have been made in both sensor technology and small Unmanned Aircraft Systems (sUAS). Improved sensor technology has provided users with cheaper, lighter, and higher resolution imaging tools, while new sUAS platforms have become cheaper, more stable and easier to navigate both manually and programmatically. These enhancements have provided remote sensing solutions for both commercial and research applications that were previously unachievable. However, this has provided non-scientific practitioners with access to technology and techniques previously only available to remote sensing professionals, sometimes leading to improper diagnoses and results. The work accomplished in this dissertation demonstrates the …


Methods And Algorithms For Cardiovascular Hemodynamics With Applications To Noninvasive Monitoring Of Proximal Blood Pressure And Cardiac Output Using Pulse Transit Time, Yashar Seyed Vahedein Dec 2019

Methods And Algorithms For Cardiovascular Hemodynamics With Applications To Noninvasive Monitoring Of Proximal Blood Pressure And Cardiac Output Using Pulse Transit Time, Yashar Seyed Vahedein

Theses

Advanced health monitoring and diagnostics technology are essential to reduce the unrivaled number of human fatalities due to cardiovascular diseases (CVDs). Traditionally, gold standard CVD diagnosis involves direct measurements of the aortic blood pressure (central BP) and flow by cardiac catheterization, which can lead to certain complications. Understanding the inner-workings of the cardiovascular system through patient-specific cardiovascular modeling can provide new means to CVD diagnosis and relating treatment. BP and flow waves propagate back and forth from heart to the peripheral sites, while carrying information about the properties of the arterial network. Their speed of propagation, magnitude and shape are …


Evaluating Antipsychotic Prescribing In The Intensive Care Unit And Across The Continuum Of Care, Michael Pasqualicchio, Heidi Clarke Dec 2019

Evaluating Antipsychotic Prescribing In The Intensive Care Unit And Across The Continuum Of Care, Michael Pasqualicchio, Heidi Clarke

All Publications

Purpose/Background: The 2018 Clinical Practice Guidelines for the Prevention and Management of Pain, Agitation/Sedation, Delirium, Immobility, and Sleep Disruption in Adult Patients in the ICU (PADIS) recommend against both typical and atypical antipsychotic use for delirium in critically ill patients. This recommendation is based on evidence showing that these medications are not associated with shorter durations of delirium, mechanical ventilation, intensive care unit (ICU) length of stay, or reduced mortality. There are many long term complications associated with the use of antipsychotics including QTc prolongation, extrapyramidal symptoms, metabolic abnormalities, and anticholinergic effects. Regardless, antipsychotics remain the most common medication used …


Prosthetic Rehabilitation Of The Geriatric Oncologic Rhinectomy Patient Utilizing A Craniofacial Implant‐Retained Nasal Prosthesis, Evan Rosen Dec 2019

Prosthetic Rehabilitation Of The Geriatric Oncologic Rhinectomy Patient Utilizing A Craniofacial Implant‐Retained Nasal Prosthesis, Evan Rosen

All Publications

No abstract provided.


Impact Of Serial Blood Glucose Monitoring And Treatment In Patients With Corticosteroid-Induced Hyperglycemia In The Inpatient Setting, Monica Tadros, Heidi Clarke, Radhan Gopalani, Stephanie Palma, Moe Shwin Dec 2019

Impact Of Serial Blood Glucose Monitoring And Treatment In Patients With Corticosteroid-Induced Hyperglycemia In The Inpatient Setting, Monica Tadros, Heidi Clarke, Radhan Gopalani, Stephanie Palma, Moe Shwin

All Publications

No abstract provided.


Retrospective Chart Review Of Patients Treated With Ibutilide For Pharmacological Cardioversion, Joyce Lee, Radhan Gopalani, Faaria Quadri Dec 2019

Retrospective Chart Review Of Patients Treated With Ibutilide For Pharmacological Cardioversion, Joyce Lee, Radhan Gopalani, Faaria Quadri

All Publications

No abstract provided.


Strategies To Assist Distance Doctoral Students In Completing Their Dissertations, Janine M. Lim, Duane Covrig, Shirley Freed, Becky De Oliveira, Mordekai Ochieng Ongo, Isadore Newman, Isadore Newman Phd Dec 2019

Strategies To Assist Distance Doctoral Students In Completing Their Dissertations, Janine M. Lim, Duane Covrig, Shirley Freed, Becky De Oliveira, Mordekai Ochieng Ongo, Isadore Newman, Isadore Newman Phd

Faculty Publications

Completing doctoral dissertations is difficult work and may be harder for distance students physically separated from institutional and collegial supports. Inability to complete independent research contributes to doctoral student attrition. Factors impacting completion include institutional factors, student characteristics, and supervisory arrangements (Manathunga, 2005). This paper shares proactive strategies used by a Midwestern university in the United States to support distance doctoral students. Strategies and technology tools are described that (a) cultivate a shared culture of responsibility and commitment, (b) increase effective communication between researchers, and (c) grow departmental and institutional services and technologies for faculty and students. This paper suggests …


Can Eccentric Exercise Of The Lower Limb Be Made More Efficiently, A Pilot Study, Jerrold S. Petrofsky, Robert Donatelli, James M. Mckivigan, Michael S. Laymon Dec 2019

Can Eccentric Exercise Of The Lower Limb Be Made More Efficiently, A Pilot Study, Jerrold S. Petrofsky, Robert Donatelli, James M. Mckivigan, Michael S. Laymon

College of Health & Human Services (TUN) Publications and Research

Abstract Background: Eccentric Exercise has been shown to be more effective in building muscle and healing damaged tissue than concentric or isometric exercise. It has also been shown to be effective in increasing motor control. But the duration of therapeutic exercise in physical therapy is limited by insurance to 30-60 minutes a day. Objectives: Four standard therapy eccentric exercises of the lower limbs were compared (toe raise, ball exercise, side lying eccentric exercise and incline board exercise) to a trainer called the BTE Eccentron to see if the efficiency of exercise could be increased using one exercise session to meet …


Instagramable Politics: Indonesian Celebrities Politicians Campaign, Nur'annafi Farni Syam Maella, Rd. Funny Mustika Elita, Edwin Rijal, Slamet Mulyana Dec 2019

Instagramable Politics: Indonesian Celebrities Politicians Campaign, Nur'annafi Farni Syam Maella, Rd. Funny Mustika Elita, Edwin Rijal, Slamet Mulyana

Library Philosophy and Practice (e-journal)

In the digital era, Instagram is used by celebrities to participate in political contestation. Starting from incumbent celebrities to new celebrities who are running for politics, they use Instagram as a campaign media in the 2019 election. The presence of Instagram is a new alternative to revolutionizing political campaigns. Their popularity as a celebrity and celebgram made it easier for them to be able to built their image themselves and be able to win votes in their constituents. This study aims to see the use of Instagram by Indonesian celebrity politicians in influencing public attitudes and views that not only …


Phosphorus Runoff Risk Assessment In Karstic Regions Of The United States, Andrew N. Sharpley, Phillip D. Hays, Karl W. Vandevender, Mike B. Daniels Dec 2019

Phosphorus Runoff Risk Assessment In Karstic Regions Of The United States, Andrew N. Sharpley, Phillip D. Hays, Karl W. Vandevender, Mike B. Daniels

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

The Phosphorus (P) Index risk assessment tool has been widely adopted across the United States to identify and rank site vulnerability to P runoff as part of the Natural Resources Conservation Service nutrient management planning (NMP) process. However, limited success has been achieved in addressing the risk of P loss by subsurface flow pathways, despite its relative importance in certain areas of the United States, particularly in those U.S. states dominated by karst terrain. Here we review how states with varying land areas classified as having karst features address the risk of P runoff during the NMP process. Indices adopted …


Effects Of Environmentally Relevant Concentrations Of Microplastic Fibers On Pacific Mole Crab (Emerita Analoga) Mortality And Reproduction, Dorothy Horn, Elise F. Granek, Clare Steele Dec 2019

Effects Of Environmentally Relevant Concentrations Of Microplastic Fibers On Pacific Mole Crab (Emerita Analoga) Mortality And Reproduction, Dorothy Horn, Elise F. Granek, Clare Steele

Environmental Science and Management Faculty Publications and Presentations

Microplastics are ubiquitous in marine systems; however, knowledge of the effects of these particles on marine fauna is limited. Ocean-borne plastic debris accumulates in littoral ecosystems worldwide, and invertebrate infauna inhabiting these systems can ingest small plastic particles and fibers, mistaking them for food. We examined the effect of microplastic fibers on physiological and reproductive outcomes in a nearshore organism by exposing Pacific mole crabs (Emerita analoga) to environmentally relevant concentrations of microsized polypropylene rope fibers. We compared adult gravid female crab mortality, reproductive success, and embryonic developmental rates between microfiber-exposed and control crabs. Pacific mole crabs exposed to polypropylene …


Stem And The Skilled Labor Shortage In The United States, Nicolo Tetsuo Hoshizaki Dec 2019

Stem And The Skilled Labor Shortage In The United States, Nicolo Tetsuo Hoshizaki

Construction Management

There is a growing shortage of skilled labor in the United States construction industry. As the baby boomer generation prepares to leave the workforce, this problem is getting exponentially worse. As Science, Technology, Engineering and Mathematics programs have become the U.S. Department of Education’s top priority, more young adults are pursuing higher education and entering the STEM workforce. Using quantitative and qualitative research methodologies, this paper provides an explanation as to why a logical method to assuage the labor shortage in construction is to employ vocational programs that prepare young adults to enter the industry similar to how STEM programs …


Increasing The Accessibility Of Bim Training Within The Aec Industry, Xavier Caldera Dec 2019

Increasing The Accessibility Of Bim Training Within The Aec Industry, Xavier Caldera

Construction Management

At the turn of the 21st century, the rise of new technology has helped modernize the AEC industry. Architects, engineers, general contractors, and foremen can build, collaborate, and communicate more efficiently using BIM to help facilitate the building process. The benefits of BIM have been proven through increased coordination, risk mitigation, and cost optimization. However, with the increasing use of BIM, there is a growing education gap between construction workers and BIM technology. Currently, there are three main avenues to learn BIM: in-house company training, attending college, or paying to learn independently online. The foundation for a BIM learning …


From Clinical Phenotype To Genotypic Modelling: Incidence And Prevalence Of Recessive Dystrophic Epidermolysis Bullosa (Rdeb), Shaundra Eichstadt, Jean Y. Tang, Daniel C. Solis, M. Peter Marinkovich, Nedra Whitehead, Fang Fang, Stephen W. Erickson, Mary E. Ritchey, Max Colao, Kaye Spratt, Amir Shaygan, Mark Ahn, Kavita Y. Sarin Dec 2019

From Clinical Phenotype To Genotypic Modelling: Incidence And Prevalence Of Recessive Dystrophic Epidermolysis Bullosa (Rdeb), Shaundra Eichstadt, Jean Y. Tang, Daniel C. Solis, M. Peter Marinkovich, Nedra Whitehead, Fang Fang, Stephen W. Erickson, Mary E. Ritchey, Max Colao, Kaye Spratt, Amir Shaygan, Mark Ahn, Kavita Y. Sarin

Engineering and Technology Management Faculty Publications and Presentations

Background: Recessive dystrophic epidermolysis bullosa (RDEB) is an inherited genetic disorder characterized by recurrent and chronic open wounds with significant morbidity, impaired quality of life, and early mortality. RDEB patients demonstrate reduction or structural alteration type VII collagen (C7) owing to mutations in the gene COL7A1, the main component of anchoring fibrils (AF) necessary to maintain epidermal-dermal cohesion. While over 700 alterations in COL7A1 have been reported to cause dystrophic epidermolysis bullosa (DEB), which may be inherited in an autosomal dominant (DDEB) or autosomal recessive pattern (RDEB), the incidence and prevalence of RDEB is not well defined. To date, the …


Feasibility And Acceptability Of A Rural, Pragmatic, Telemedicine‐ Delivered Healthy Lifestyle Programme, John A. Batsis, Auden C. Mcclure, Aaron B. Weintraub, David F. Kotz, Sivan Rotenberg, Summer B. Cook, Diane Gilbert-Diamond, Kevin Curtis, Courtney J. Stevens, Diane Sette, Richard I. Rothstein Dec 2019

Feasibility And Acceptability Of A Rural, Pragmatic, Telemedicine‐ Delivered Healthy Lifestyle Programme, John A. Batsis, Auden C. Mcclure, Aaron B. Weintraub, David F. Kotz, Sivan Rotenberg, Summer B. Cook, Diane Gilbert-Diamond, Kevin Curtis, Courtney J. Stevens, Diane Sette, Richard I. Rothstein

Dartmouth Scholarship

Background: The public health crisis of obesity leads to increasing morbidity that are even more profound in certain populations such as rural adults. Live, two‐way video‐conferencing is a modality that can potentially surmount geographic barriers and staffing shortages. Methods: Patients from the Dartmouth‐Hitchcock Weight and Wellness Center were recruited into a pragmatic, single‐arm, nonrandomized study of a remotely delivered 16‐week evidence‐based healthy lifestyle programme. Patients were provided hardware and appropriate software allowing for remote participation in all sessions, outside of the clinic setting. Our primary outcomes were feasibility and acceptability of the telemedicine intervention, as well as potential effectiveness on …


Exploring The State-Of-Receptivity For Mhealth Interventions, Florian Künzler, Varun Mishra, Jan-Niklas Kramer, David Kotz, Elgar Fleisch, Tobias Kowatsch Dec 2019

Exploring The State-Of-Receptivity For Mhealth Interventions, Florian Künzler, Varun Mishra, Jan-Niklas Kramer, David Kotz, Elgar Fleisch, Tobias Kowatsch

Dartmouth Scholarship

Recent advancements in sensing techniques for mHealth applications have led to successful development and deployments of several mHealth intervention designs, including Just-In-Time Adaptive Interventions (JITAI). JITAIs show great potential because they aim to provide the right type and amount of support, at the right time. Timing the delivery of a JITAI such as the user is receptive and available to engage with the intervention is crucial for a JITAI to succeed. Although previous research has extensively explored the role of context in users’ responsiveness towards generic phone notiications, it has not been thoroughly explored for actual mHealth interventions. In this …


Percutaneous Venoplasty For Central Venous Stenosis: Effect On Patient's Symptoms And Patency Of Arteriovenous Accesses, Zia Ur Rehman, Abdul Hakeem Memon, Raza Sayani, Nida Zahid Dec 2019

Percutaneous Venoplasty For Central Venous Stenosis: Effect On Patient's Symptoms And Patency Of Arteriovenous Accesses, Zia Ur Rehman, Abdul Hakeem Memon, Raza Sayani, Nida Zahid

Department of Surgery Docs

Objective: To determine symptomatic relief and patency rate of arteriovenous (AV) fistulae and grafts after venoplasty in patients with central venous stenosis (CVS) on hemodialysis.
Study Design: Case series.
Place and Duration of Study: The Aga Khan University Hospital, Karachi, from January 2012 to December 2017.
Methodology: The data of patients who had one session of successful venoplasty for CVS were reviewed. The outcomes measured were symptomatic recovery and improvement in the patency of AV accesses. Symptomatic recovery was termed 'complete', when there was complete symptomatic relief after venoplasty; and 'partial' when the procedure was technically successful, but symptoms were …


Structural Basis Of Amino Acid Surveillance By Higher-Order Trna-Mrna Interactions, Shuang Li, Zhaoming Su, Jean Lehmann, Vassiliki Stamatopoulou, Nikoleta Giarimoglou, Frances E Henderson, Lixin Fan, Grigore D Pintilie, Kaiming Zhang, Muyuan Chen, Steven J Ludtke, Yun-Xing Wang, Constantinos Stathopoulos, Wah Chiu, Jinwei Zhang Dec 2019

Structural Basis Of Amino Acid Surveillance By Higher-Order Trna-Mrna Interactions, Shuang Li, Zhaoming Su, Jean Lehmann, Vassiliki Stamatopoulou, Nikoleta Giarimoglou, Frances E Henderson, Lixin Fan, Grigore D Pintilie, Kaiming Zhang, Muyuan Chen, Steven J Ludtke, Yun-Xing Wang, Constantinos Stathopoulos, Wah Chiu, Jinwei Zhang

Faculty, Staff and Students Publications

Amino acid availability in Gram-positive bacteria is monitored by T-box riboswitches. T-boxes directly bind tRNAs, assess their aminoacylation state, and regulate the transcription or translation of downstream genes to maintain nutritional homeostasis. Here, we report cocrystal and cryo-EM structures of Geobacillus kaustophilus and Bacillus subtilis T-box-tRNA complexes, detailing their multivalent, exquisitely selective interactions. The T-box forms a U-shaped molecular vise that clamps the tRNA, captures its 3' end using an elaborate 'discriminator' structure, and interrogates its aminoacylation state using a steric filter fashioned from a wobble base pair. In the absence of aminoacylation, T-boxes clutch tRNAs and form a continuously …