Digital Commons Network^™

Nanopower Analog Frontends For Cyber-Physical Systems, Kenji Aono

McKelvey School of Engineering Graduate Student Theses & Dissertations

In a world that is increasingly dominated by advances made in digital systems, this work will explore the exploiting of naturally occurring physical phenomena to pave the way towards a self-powered sensor for Cyber-Physical Systems (CPS). In general, a sensor frontend can be broken up into a handful of basic stages: transduction, filtering, energy conversion, measurement, and interfacing. One analog artifact that was investigated for filtering was the physical phenomenon of hysteresis induced in current-mode biquads driven near or at their saturation limit. Known as jump resonance, this analog construct facilitates a higher quality factor to be brought about without …

Combining Footprinting Mass Spectrometry And Molecular Dynamics Simulation For Structural Studies In Membrane Proteins, Fengbo Zhou

Arts & Sciences Graduate Student Theses and Dissertations

Membrane proteins are essential in many cellular processes and represent ~60% of all current drug targets. Due to technical limits, membrane proteins of various types were not studied extensively in the past and the biochemistry and functionality of them remain unclear. The structural biology methodologies require pure isolated protein samples for us to resolve their structure and study their biochemical functions. For such in vitro studies, however, membrane proteins often become unstable when isolated from their native lipid bilayer environment. To overcome the challenge, I employed a novel methodology of solubilizing membrane proteins in solution without detergent. I reconstituted ferroportin, …

The Essential Roles Of The Chromatin Factor Gon4l In Heart Development, Terin Elise Budine

Arts & Sciences Graduate Student Theses and Dissertations

Heart development and the genetic pathways underlying it are highly conserved among vertebrates. During heart development, an embryo must induce mesoderm formation, pattern the mesoderm, specify cardiomyocytes, increase the population of cardiomyocytes through proliferation, and pattern the cardiac chambers. It is becoming increasingly clear that chromatin modifications help mediate the complex processes of heart development by providing spatiotemporal regulation of gene expression. My thesis work focuses on characterizing functions of the chromatin factor Gonad-4-like (Gon4l), encoded by the gene ugly duckling (udu), in zebrafish heart development. Previous works established a requirement for Gon4l in the formation of many mesoderm derivatives …

Protein Structure-Guided Approaches To Identify Functional Mutations In Cancer, Sohini Sengupta

Arts & Sciences Graduate Student Theses and Dissertations

Distinguishing driver mutations from passenger mutations within tumor cells continues to be a major challenge in cancer genomics. Many computational tools have been developed to address this challenge; however, they rely heavily on primary protein sequence context and frequency/mutation rate. Rare driver mutations not found in many cancer patients may be missed with these traditional approaches. Additionally, the structural context of mutations on tertiary/quaternary protein structures is not taken into account and may play a more prominent role in determining phenotype and function. This dissertation first presents a novel computational tool called HotSpot3D, which identifies regions of protein structures that …

Modeling The Bioactivation And Subsequent Reactivity Of Drugs, Tyler Brian Hughes

Arts & Sciences Graduate Student Theses and Dissertations

Metabolism can convert drugs to harmful reactive metabolites that conjugate to DNA and off-target proteins. Reactive metabolites are a significant driver of both drug candidate attrition and withdrawal from the market of already approved drugs. Unfortunately, reactive metabolites are difficult to study in vivo, because they are transitory and generally do not circulate. Instead, this work computationally models both metabolism and reactivity. Using deep learning, predictive models were developed for the metabolic formation of quinones and epoxides, which together account for about half of known reactive metabolites. Additionally, an accurate model of DNA and protein reactivity was constructed, which predicts …

A Mouse Model Of Börjeson-Forssman-Lehmann Syndrome Reveals A Potential Link With Autism Spectrum Disorder, Cheng Cheng

Arts & Sciences Graduate Student Theses and Dissertations

ABSTRACT OF THE DISSERTATIONA Mouse Model of Börjeson-Forssman-Lehmann Syndrome reveals a potential link with Autism Spectrum DisorderByCheng ChengDoctor of Philosophy in Biology and Biomedical SciencesDevelopmental, Regenerative and Stem Cell BiologyWashington University in St. Louis, 2018Dr. Azad Bonni, ChairIntellectual disability (ID) is a prevalent neurodevelopmental disorder that affects 1% to 3% of the general population. ID is characterized by developmental deficiencies in cognitive function and adaptive behaviors. Lacking effective treatments, ID currently presents an immense burden to affected families and the economy. Therefore, there is an urgent need to elucidate the pathogenesis of ID. Human genetic studies have associated ID with …

Genetic Basis Of Thermal Divergence In Saccharomyces Species, Xueying C. Li

Arts & Sciences Graduate Student Theses and Dissertations

The genetic architecture of phenotypic divergence is a central question in evolutionary biology. Genetic architecture is impacted by whether evolution occurs through accumulation of many small-effect or a few large-effect changes, the relative contribution of coding and cis-regulatory changes, and the prevalence of epistatic effects. Our empirical understanding of the genetic basis of evolutionary change remains incomplete, largely because reproductive barriers limit genetic analysis to those phenotypes that distinguish closely related species. In this dissertation, I use hybrid genetic analysis to examine the basis of thermal divergence between two post-zygotically isolated species, Saccharomyces cerevisiae and S. uvarum. S. cerevisiae is …

A Recombinant Virus And Reporter Mouse System To Study Chronic Chikungunya Virus Pathogenesis, Alissa Roxanne Young

Arts & Sciences Graduate Student Theses and Dissertations

Chikungunya virus (CHIKV) is an arthritogenic alphavirus that during acute disease causes fever as well as severe joint and muscle pain. Chronic joint and muscle pain persists in a significant subset of patients, yet we still have a poor understanding of what drives this chronic disease. While replicating virus has not been detected in the joints of patients with chronic arthritis or in various animal models at chronic time points, persistent viral RNA can be detected for months after acute infection.To identify the cells that could be contributing to chronic CHIKV pathogenesis, we developed recombinant viruses that express Cre recombinase …

The Role Of Apolipoprotein E In Regulating Tau Pathogenesis And Neurodegeneration In A Tauopathy Mouse Model, Yang Shi

Arts & Sciences Graduate Student Theses and Dissertations

APOE4 is the strongest genetic risk factor for late-onset Alzheimer's disease (AD). APOE4 increases brain amyloid-β (Aβ) pathology relative to other APOE isoforms. However, whether APOE independently influences tau pathology, the other pathological hallmark of AD and other tauopathies, or tau-mediated neurodegeneration, is not clear. By generating P301S tau transgenic mice on either a human APOE knock in (KI) or APOE knockout (KO) background, we show that the presence of human APOE, regardless of APOE isoforms, leads to various degrees of brain atrophy in 9-month old P301S mice, whereas APOE ablation strongly protects against neurodegeneration. In particular, P301S/E4 mice develop …

Fast Objective Coupled Planar Illumination Microscopy, Cody Jonathan Greer

Arts & Sciences Graduate Student Theses and Dissertations

Among optical imaging techniques light sheet fluorescence microscopy stands out as one of the most attractive for capturing high-speed biological dynamics unfolding in three dimensions. The technique is potentially millions of times faster than point-scanning techniques such as two-photon microscopy. This potential is especially poignant for neuroscience applications due to the fact that interactions between neurons transpire over mere milliseconds within tissue volumes spanning hundreds of cubic microns. However current-generation light sheet microscopes are limited by volume scanning rate and/or camera frame rate. We begin by reviewing the optical principles underlying light sheet fluorescence microscopy and the origin of these …

Anodic Cyclization Reaction: Manipulation Of Reaction Pathway And Efforts To Radical Cation And Radical Intermediate, Ruozhu Feng

Arts & Sciences Graduate Student Theses and Dissertations

In recent years, synthetic chemists have been expressing significant interest in electro-organic synthetic methods. This interest is being fueled by the existence of an increasing number of successful methods in the literature and the availability of new electrochemical equipment that removes the barrier to attempting an electrolysis reaction for the first time. Yet while these developments have fueled growth in some areas of electro-organic synthesis (the recycling of chemical catalysts for example), other areas remain underdeveloped. One such area is the exploration of reactions that can be triggered directly as an electrode surface without the use of any chemical reagent. …

Freezing Of Gait: Mechanisms, Mechanics, And Management, Peter S. Myers

Arts & Sciences Graduate Student Theses and Dissertations

Parkinson disease (PD) is a neurodegenerative disease with multiple motor and non-motor symptoms, including postural instability, gait impairments, and cognitive deficits. More than 50% of individuals with PD experience a symptom called freezing of gait (FOG), described as a transient inability to take another step forward. Individuals with PD who experience FOG (freezers) have further postural, gait, and cognitive impairments compared to individuals with PD without FOG (non-freezers). While degeneration of the dopaminergic neurons in the substantia nigra is accepted as the primary etiology of the disease, research shows that the disease has a global impact on the brain, accounting …

Neural Mechanisms Of Drosophila Circadian Rhythms, Xitong Liang

Arts & Sciences Graduate Student Theses and Dissertations

Animals show circadian rhythms in a variety of physiological functions and behaviors. In Drosophila melanogaster, behavioral rhythms are driven by circadian clock genes that are oscillating in ~150 circadian pacemaker neurons. To explain how circadian neurons encode time and regulate different behavioral rhythms, I performed 24-hour in vivo whole-brain calcium imaging using light-sheet microscopy. First, I found that different groups of circadian neurons show circadian rhythms in spontaneous neural activity with diverse phases. The neural activity phases of the M and E pacemaker groups, which are associated with the morning and evening locomotor activities respectively, occur ~4 hours before their …

Singing As A Therapeutic Technique To Improve Gait For People With Parkinson Disease, Elinor Harrison

Arts & Sciences Graduate Student Theses and Dissertations

Abstract of the DissertationSinging as a Therapeutic Technique toImprove Gait for People with Parkinson DiseasebyElinor Clare HarrisonDoctor of Philosophy in Movement ScienceNeurosciencesWashington University in St. Louis, 2018Professor Gammon Earhart, ChairGait impairment is common in older adults and even more prevalent for people with Parkinson disease (PD). Gait dysfunction is often characterized by reductions in speed, step frequency, and step length. In addition, decreased ability to regulate step length and step frequency may contribute to increased gait variability, making walking less stable and increasing risk for falls. As gait deficits are often resistant to drug therapy, there is a need to …

The Effect Of Talker And Contextual Variability On Memory For Words In Sentences, Nichole Runge

Arts & Sciences Graduate Student Theses and Dissertations

Previous research has found that adding different forms of variability during study can affect later memory at test. For example, having words spoken by different talkers has been shown to improve recall of known and novel words (Goldinger et al., 1999; Barcroft & Sommers, 2005), and varying the cues in cue-target related word pairs has been found to improve recall of the targets (Glenberg, 1979; Bevan et al., 1966). It was unclear, however, whether benefits of variability would extend to more naturalistic stimuli, such as sentences, which have higher working memory demands. The present set of experiments investigated how talker …

Linking Structure And Dynamics In Metallic Liquids: A Combined Experimental And Molecular Dynamics Approach, Robert Ashcraft

Arts & Sciences Graduate Student Theses and Dissertations

A major outstanding problem in condensed matter physics is the nature of the glass transition, in which a rapidly cooled liquid can bypass the transition into a crystalline state and the liquid structure is "frozen-in" due to kinetic arrest. To characterize the fundamental features behind this transition the liquid, both in the high temperature (equilibrium) and supercooled state, needs to be better understood. By examining the relationship between structure and dynamics a better characterization of the liquid state and a determination of the mechanisms that are ultimately important for the formation of the glass can be gained. In this dissertation, …

Corporate Social Responsibility To-Come: A Derridean Interruption Of Transparency, Robert Foschia

Electronic Theses and Dissertations

This study investigated the relation between rhetorics of transparency and organizational action. Digging into CSR literature from a philosophy of communication perspective, this project seeks to determine if corporate social responsibility delivers on the promises it makes of a better world.

Drawing on the work of Jacques Derrida, the research first lays out his often overlooked contribution to the philosophy of communication, and then moves towards possible applications in deconstructing the perceived benefits of CSR, particularly in its transparent nature. By looking at organizational life from the Triple Bottom Line, this dissertation peels back the underlying rhetoric of planet, people, …

Georgia Southern Wins Camellia Bowl In Dramatic Fashion, Georgia Southern University

Athletics: News & Publications

• Georgia Southern Wins Camellia Bowl in Dramatic Fashion

"Who Are These Wild Indians": On The Foreign Policies Of Some Voluntarily Isolated Peoples In Amazonia, Peter Gow

Tipití: Journal of the Society for the Anthropology of Lowland South America

This paper is a reflection on the phenomenon of voluntary isolation in Amazonia, about anthropology’s implication in its formation as a concept, and what anthropologists might profitably say about it as a concrete phenomenon in the world. While knowledge based on ethnographic fieldwork might by minimal or even totally absent for people in voluntary isolation, anthropological research has produced a very impressive understanding of indigenous Amazonian social forms in general, knowledge that can be brought to bear on the question.

O Papel Dos Povos Indígenas Isolados Na Efetivação De Seus Direitos: Apontamentos Para O Reconhecimento De Suas Estratégias De Vida, Fabrício Amorim

Tipití: Journal of the Society for the Anthropology of Lowland South America

Este artigo discute conceitos e apresenta reflexões sobre questões relacionadas aos povos indígenas isolados no Brasil a partir de trajetória profissional do autor junto à temática. Aborda diferentes temas relacionados aos povos isolados, tal como “vulnerabilidade,” “isolamento voluntário” e “políticas públicas,” com o objetivo de informar e descontruir equívocos que a sociedade em geral possui sobre esses temas. Argumenta que os povos isolados são coletivos ativos na construção e garantia de direitos indígenas por meio de processos de resistência, e que suas estratégias de isolamento devem ser reconhecidas como expressão máxima de sua autonomia.

This article presents a series of …

Sobre La Antropología Amazónica (Amazonista) En El Perú: Comentarios Al Texto De Jean-Pierre Chaumeil, Oscar Espinosa

Tipití: Journal of the Society for the Anthropology of Lowland South America

Commentary on the Opening Lecture, “A Window into Twenty Years of Amazonianist Anthropology in Peru (1997–2017)” proffered by Jean-Pierre Chaumeil at the XI Salsa conference and featured in the previous volume of Tipití (15:105–117).

Werken By Bernardo Oyarzún (Mapuche), Ana Guevara, Sophie Moiroux

Tipití: Journal of the Society for the Anthropology of Lowland South America

No abstract provided.

Good Reasons Or Bad Conscience: A Postscript, Stephen P. Hugh-Jones

Tipití: Journal of the Society for the Anthropology of Lowland South America

Published in French in 1996, the original article for which this comprises a post-script set indigenous Amazonians’ attitudes to meat alongside those of Euro-Americans. With the accelerating deforestation of Amazonia linked with the cultivation of soya used to feed animals for meat, and with calls to reduce or abandon meat consumption as one way of averting catastrophic climate change, it is topical once again. In this postscript, I reply to two contrasting critiques of the article, the first wary of an excess of ontology, the second distrustful of a deficit of it. Does a focus on ritual and shamanism obscure …

Qwerty, James E. Fowler

Westview

What you’ve seen but can’t place, always overlooking

Big, James E. Fowler

Westview

The setting sun casts a Giacometti shadow before me.

2018-December-15-New Employees, University Marketing And Communications, East Tennessee State University

ETSU News

No abstract provided.

2018-December-15-Percipio, University Marketing And Communications, East Tennessee State University

ETSU News

No abstract provided.

The Library Book, Kylie Bean

Departmental Honors & Graduate Capstone Projects

I wrote my novella, The Library Book, in partial fulfillment of my creative writing seminar. The majority of the coursework of this seminar consisted of writing and revising an entire novella from initial conception and outlining to final revisions. I am submitting my revised copy of The Library Book, which I submitted as the final project of my ENGL 470B seminar. I received an A on this project and will be reading a section of my novella at the 2019 Kemp Symposium this Friday. The Library Book is a forty-eight-page fantasy novella written for a middle grade audience. …

Neural And Behavioral Associations Of Drinking And Empathic Processing, Charles Samuel Henry Robinson

Psychology ETDs

Alcohol Use Disorder (AUD) is a major health problem, yet most individuals with AUD do not perceive a need for formal treatment and do not receive treatment. The lack of treatment seeking among individuals with AUD may suggest a lack of self-awareness and insight into the seriousness of AUD related problems. Moreover, individuals who have lost or are at risk of losing major relationships are especially likely to perceive the need for help. Thus, it may be the case that awareness of the influence of one’s drinking on social relationships and the feelings of others (i.e., empathy) could improve treatment …

Making Space For Makerspace: How Adding A Makerspace Can Benefit Art Museum Education Programming, Kaeleigh Thorp

Master's Projects and Capstones

In this capstone project, I advocate for adding makerspaces to art museum education programming. I review the foundational educational concepts of a makerspace which help explain why it is a modern strategy to increasing visitor learning. This includes John Dewey’s theories of experience and education, Howard Gardner’s multiple intelligences theory, George Hein’s studies of museum visitor behavior, and the physiological science of learning. I then propose four key terms essential for visitor learning, and explain how makerspaces successfully incorporate them. The four key terms are: 1. multiple intelligences, 2. direct experience, 3. inquiry based and open ended learning, and 4. …