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Patterns And Drivers Of Recent Disturbances Across The Temperate Forest Biome, Andreas Sommerfeld, Cornelius Senf, Brian Buma, Anthony W. D’Amato, Tiphaine Després, Ignacio Díaz-Hormazábal, Shawn Fraver, Lee E. Frelich, Álvaro G. Gutiérrez, Sarah J. Hart, Brian J. Harvey, Hong S. He, Tomáš Hlásny, Andrés Holz, Thomas Kitzberger, Dominik Kulakowski, David Lindenmayer, Akira S. Mori, Jörg Müller, Juan Paritsis, George L.W. Perry, Scott L. Stephens, Miroslav Svoboda, Monica G. Turner, Thomas T. Veblen, Rupert Seidl

Geography

Increasing evidence indicates that forest disturbances are changing in response to global change, yet local variability in disturbance remains high. We quantified this considerable variability and analyzed whether recent disturbance episodes around the globe were consistently driven by climate, and if human influence modulates patterns of forest disturbance. We combined remote sensing data on recent (2001–2014) disturbances with in-depth local information for 50 protected landscapes and their surroundings across the temperate biome. Disturbance patterns are highly variable, and shaped by variation in disturbance agents and traits of prevailing tree species. However, high disturbance activity is consistently linked to warmer and …

Beauty Is In The Eye Of The Beer Holder: An Initial Investigation Of The Effects Of Alcohol, Attractiveness, Warmth, And Competence On The Objectifying Gaze In Men, Abigail R. Riemer, Michelle Haikalis, Molly R. Franz, Michael Dodd, David Dilillo, Sarah Gervais

Department of Psychology: Faculty Publications

Despite literature revealing the adverse consequences of objectifying gazes for women, little work has empirically examined origins of objectifying gazes by perceivers. Integrating alcohol myopia and objectification theories, we examined the effects of alcohol as well as perceived female attractiveness, warmth, and competence on objectifying gazes. Specifically, male undergraduates (n = 49) from a large U.S. Midwestern university were administered either an alcoholic or placebo beverage. After consumption, participants were asked to focus on the appearance or personality (counterbalanced) of pictured women who were previously rated as high, average, or low in attractiveness, warmth, and competence. Replicating previous work, appearance …

A Behavioral Mechanistic Investigation Of The Role Of 5-Ht1a Receptors In The Mediation Of Rat Maternal Behavior, Xiaonan Li, Xiaojing Ding, Ruiyong Wu, Leilei Chen, Jun Gao, Gang Hu, Ming Li

Department of Psychology: Faculty Publications

Previous work suggests that 5-HT_1A receptors play a special role in rodent maternal aggression, but not in other aspects of maternal care (e.g. pup retrieval and nest building). The present study re-assessed the basic effects of 5-HT_1A activation or blockade on various maternal responses in postpartum female rats. We also examined the possible behavioral mechanisms underlying the maternal effects of 5-HT_1A. Sprague–Dawley mother rats were injected with a 5-HT_1A agonist 8-OH-DPAT (0.1, 0.5 or 1.0 mg/kg, sc), a 5-HT_1A antagonist WAY-101405 (0.1, 0.5 or 1.0 mg/kg, sc) or 0.9% saline solution on postpartum days …

Microrna Cluster Mir199a/214 Are Differentially Expressed In Female And Male Rats Following Nicotine Self-Administration, Steven T. Pittenger, Victoria L. Schaal, Dalia Moore, Rahul S. Guda, Sneh Koul, Sowmya V. Yelamanchili, Rick A. Bevins, Gurudutt Pendyala

Department of Psychology: Faculty Publications

Previous research has established sex differences associated with nicotine intake, however a significant gap in knowledge remains regarding the molecular mechanisms that govern these differences at the transcriptional level. One critical regulator of transcription are microRNAs (miRNAs). miRNAs are a family of non-coding RNAs that regulate an array of important biological functions altered in several disease states, including neuroadaptive changes within the brain associated with drug dependence. We examined the prefrontal cortex (PFC) from male and female Sprague-Dawley rats following self-administration (22 days) of nicotine or yoked saline controls using next generation RNA-Sequencing (RNA-Seq) technology and found an array of …

Individual Differences In Valence Bias: Fmri Evidence Of The Initial Negativity Hypothesis, Nathan M. Petro, Tien T. Tong, Daniel J. Henley, Maital Neta

Department of Psychology: Faculty Publications

Facial expressions offer an ecologically valid model for examining individual differences in affective decision-making. They convey an emotional signal from a social agent and provide important predictive information about one’s environment (presence of potential rewards or threats). Although some expressions provide clear predictive information (angry, happy), others (surprised) are ambiguous in that they predict both positive and negative outcomes. Thus, surprised faces can delineate an individual’s valence bias, or the tendency to interpret ambiguity as positive or negative. Our initial negativity hypothesis suggests that the initial response to ambiguity is negative, and that positivity relies on emotion regulation. We tested …

Age Of Migration Differentials In Life Expectancy With Cognitive Impairment: 20-Year Findings From The Hispanic-Epese, Marc A. Garcia, Joseph L. Saenz, Brian Downer, Chi-Tsun Chiu, Sunshine Rote, Rebeca Wong

Department of Sociology: Faculty Publications

Background and Objectives To examine differences in life expectancy with cognitive impairment among older Mexican adults according to nativity (U.S.-born/foreign-born) and among immigrants, age of migration to the United States.

Research Design and Methods This study employs 20 years of data from the Hispanic Established Populations for the Epidemiologic Study of the Elderly to estimate the proportion of life spent cognitively healthy and cognitively impaired prior to death among older Mexican adults residing in the southwestern United States. We combine age-specific mortality rates with age-specific prevalence of cognitive impairment, defined as a Mini-Mental Status Exam score of less than 21 …

Characterization Of Solution-Phase Drug-Protein Interactions By Ultrafast Affinity Extraction, Sandya Beeram, Xiwei Zheng, Kyungah Suh, David S. Hage

Department of Chemistry: Faculty Publications

A number of tools based on high-performance affinity separations have been developed for studying drug-protein interactions. An example of one recent approach is ultrafast affinity extraction. This method has been employed to examine the free (or non-bound) fractions of drugs and other solutes in simple or complex samples that contain soluble binding agents. These free fractions have also been used to determine the binding constants and rate constants for the interactions of drugs with these soluble agents. This report describes the general principles of ultrafast affinity extraction and the experimental conditions under which it can be used to characterize such …

Systems And Methods For Actuating Soft Robotic Actuators, Robert F. Shepherd, Adam Stokes, Stephen A. Morin, Ludovico Cademartiri, Jacob Freake, Rui Nunes, Xin Chen, George M. Whitesides

Department of Chemistry: Faculty Publications

Systems and methods for providing a soft robot is provided. In one system , a robotic device includes a flexible body having a fluid chamber, where a portion of the flexible body includes an elastically extensible material and a portion of the flexible body is strain limiting relative to the elastically extensible material. The robotic device can further include a pressurizing inlet in fluid communication with the fluid chamber, and a pressurizing device in fluid communication with the pressurizing inlet, the pressurizing device including a reaction chamber configured to accommodate a gas producing chemical reaction for providing pressurized gas to …

Anatomy Of Stem Teaching In American Universities: A Snapshot From A Large-Scale Observation Study, Marilyne Stains, Jordan Harshman, Megan K. Barker, Stephanie V. Chasteen, Renee Cole, Sue Ellen Dechenne-Peters, M. Kevin Eagan Jr., Joan M. Esson, Jennifer K. Knight, Frank A. Laski, Marc Levis-Fitzgerald, Christopher J. Lee, Stanley M. Lo, Lisa M. Mcdonnell, Timothy A. Mckay, Nicole Michelotti, Michael S. Palmer, Kathryn M. Plank, Tamara M. Rodela, Erin R. Sanders, Natalie G. Schimpf, Patricia M. Schulte, Michelle Smith, Mackenzie Stetzer, Jackie Stewart, Blaire Van Valkenburgh, Erin Vinson, Laura K. Weir, Paul J. Wendel, Lindsay B. Wheeler, Anna M. Young

Department of Chemistry: Faculty Publications

National and local initiatives focused on the transformation of STEM teaching in higher education have multiplied over the last decade. These initiatives often focus on measuring change in instructional practices, but it is difficult to monitor such change without a national picture of STEM educational practices, especially as characterized by common observational instruments. We characterized a snapshot of this landscape by conducting the first large scale observation-based study. We found that lecturing was prominent throughout the undergraduate STEM curriculum, even in classrooms with infrastructure designed to support active learning, indicating that further work is required to reform STEM education. Additionally, …

Reductive Cleavage Of Organic Peroxides By Iron Salts And Thiols, Anderw Olson, Abigail J. Jameson, Shiva K. Kyasa, Boone W. Evans, Patrick H. Dussault

Department of Chemistry: Faculty Publications

Despite the low bond strength of the oxygen− oxygen bond, organic peroxides are often surprisingly resistant to cleavage by nucleophiles and reductants. As a result, achieving decomposition under mild conditions can be challenging. Herein, we explore the reactivity of a selection of peroxides toward thiolates, phenyl selenide, Fe(II) salts, and iron thiolates. Peroxides activated by conjugation, strain, or stereoelectronics are rapidly cleaved at room temperature by thiolate anions, phenylselenide, or Fe(II) salts. Under the same conditions, unhindered dialkyl peroxides are only marginally reactive; hindered peroxides, including triacetone triperoxide and diacetone diperoxide (DADP), are inert. In contrast, all but the most …

Synbiotics For Improved Human Health: Recent Developments, Challenges, And Opportunities, Janina A. Krumbeck, Jens Walter, Robert W. Hutkins

School of Biological Sciences: Faculty Publications

Research on combining pro- and prebiotics as synbiotics to enhance human and animal health has accelerated in the past 10 years, including many clinical trials that have assessed a diverse range of synbiotic formulations. In this review, we summarize these studies as well as the commercial applications of synbiotics that are available. In particular, we critically assess the claimed health benefits of synbiotic applications and the ecological and therapeutic factors to consider when designing synbiotics and discuss the implications of these concepts for future research in this field.

Diversity And Functional Evolution Of Terpene Synthases In Dictyostelid Social Amoebae, Xinlu Chen, Tobias G. Kollner, Gad Shaulsky, Qidong Jia, Jeroen S. Dickschat, Jonathan Gershenzon, Feng Chen

Nebraska Center for Biotechnology: Faculty and Staff Publications

Dictyostelids, or social amoebae, have a unique life style in forming multicellular fruiting bodies from unicellular amoeboids upon starvation. Recently, dictyostelids were found to contain terpene synthase (TPS) genes, a gene type of secondary metabolism previously known to occur only in plants, fungi and bacteria. Here we report an evolutionary functional study of dictyostelid TPS genes. The number of TPS genes in six species of dictyostelids examined ranges from 1 to 19; and the model species Dictyostelium purpureum contains 12 genes. Using in vitro enzyme assays, the 12 TPS genes from D. purpureum were shown to encode functional enzymes with …

Non-Targeted Colonization By The Endomycorrhizal Fungus, Serendipita Vermifera, In Three Weeds Typically Co-Occurring With Switchgrass, Prasun Ray, Yingqing Guo, Jaydeep Kolape

Nebraska Center for Biotechnology: Faculty and Staff Publications

Serendipita vermifera (=Sebacina vermifera; isolate MAFF305830) is a mycorrhizal fungus originally isolated from the roots of an Australian orchid that we have previously shown to be beneficial in enhancing biomass yield and drought tolerance in switchgrass, an important bioenergy crop for cellulosic ethanol production in the United States. However, almost nothing is known about how this root-associated fungus proliferates and grows through the soil matrix. Such information is critical to evaluate the possibility of non-target effects, such as unintended spread to weedy plants growing near a colonized switchgrass plant in a field environment. A microcosm experiment was conducted …

Molecular Basis Of Hemoglobin Adaptation In The High-Flying Bar-Headed Goose, Chandrasekhar Natarajan, Agnieszka Jendroszek, Amit Kumar, Roy E. Weber, Jeremy R. H. Tame, Angela Fago, Jay F. Storz

School of Biological Sciences: Faculty Publications

During the adaptive evolution of a particular trait, some selectively fixed mutations may be directly causative and others may be purely compensatory. The relative contribution of these two classes of mutation to adaptive phenotypic evolution depends on the form and prevalence of mutational pleiotropy. To investigate the nature of adaptive substitutions and their pleiotropic effects, we used a protein engineering approach to characterize the molecular basis of hemoglobin (Hb) adaptation in the high-flying bar-headed goose (Anser indicus), a hypoxia-tolerant species renowned for its trans-Himalayan migratory flights. To test the effects of observed substitutions on evolutionarily relevant genetic backgrounds, …

Change In Dominance Determines Herbivore Effects On Plant Biodiversity, Sally E. Koerner, Johannes Knops, Dustin J. Strong, 80 More Co-Authors

School of Biological Sciences: Faculty Publications

Herbivores alter plant biodiversity (species richness) in many of the world's ecosystems, but the magnitude and the direction of herbivore effects on biodiversity vary widely within and among ecosystems. One current theory predicts that herbivores enhance plant biodiversity at high productivity but have the opposite effect at low productivity. Yet, empirical support for the importance of site productivity as a mediator of these herbivore impacts is equivocal. Here, we synthesize data from 252 large-herbivore exclusion studies, spanning a 20-fold range in site productivity, to test an alternative hypothesis—that herbivore-induced changes in the competitive environment determine the response of plant biodiversity …

Cd32 Is Expressed On Cells With Transcriptionally Active Hiv But Does Not Enrich For Hiv Dna In Resting T Cells, Mohamed Abdel-Mohsen, Leticia Kuri-Cervantes, Judith Grau-Exposito, Adam M. Spivak, Racheal A. Nell, Costin Tomescu, Surya Kumari Vadrevu, Leila B. Giron, Carla Serra-Peinado, Meritxell Genesca, Josep Castellvi, Guoxin Wu, Perla M. Del Rio Estrada, Mauricio Gonzalez-Navarro, Kenneth Lynn, Collin T. King, Sai Vemula, Kara Cox, Yanmin Wan, Qingsheng Li, Karam Mounzer, Jay Kostman, Ian Frank, Mirko Paiardini, Daria Hazuda, Gustavo Reyes-Teran, Douglas Richman, Bonnie Howell, Pablo Tebas, Javier Martinez-Picado, Vicente Planelles, Maria J. Buzon, Michael R. Betts, Luis J. Montaner

School of Biological Sciences: Faculty Publications

The persistence of HIV reservoirs, including latently infected, resting CD4⁺ T cells, is the major obstacle to cure HIV infection. CD32a expression was recently reported to mark CD4⁺ T cells harboring a replication-competent HIV reservoir during antiretroviral therapy (ART) suppression. We aimed to determine whether CD32 expression marks HIV latently or transcriptionally active infected CD4⁺ T cells. Using peripheral blood and lymphoid tissue of ART-treated HIV⁺ or SIV⁺ subjects, we found that most of the circulating memory CD32⁺ CD4⁺ T cells expressed markers of activation, including CD69, HLA-DR, CD25, CD38, and Ki67, and …

Multiscale Modeling Of Layer Formation In Epidermis, Huijing Du, Yangyang Wang, Daniel Haensel, Briana Lee, Xing Dai, Qing Nie

Department of Mathematics: Faculty Publications

The mammalian skin epidermis is a stratified epithelium composed of multiple layers of epithelial cells that exist in appropriate sizes and proportions, and with distinct boundaries separating each other. How the epidermis develops from a single layer of committed precursor cells to form a complex multilayered structure of multiple cell types remains elusive. Here, we construct stochastic, three-dimensional, and multiscale models consisting of a lineage of multiple cell types to study the control of epidermal development. Symmetric and asymmetric cell divisions, stochastic cell fate transitions within the lineage, extracellular morphogens, cell-to-cell adhesion forces, and cell signaling are included in model. …

Modeling Craniofacial Development Reveals Spatiotemporal Constraints On Robust Patterning Of The Mandibular Arch, Lina Meinecke, Praveer P. Sharma, Huijing Du, Lei Zhang, Qing Nie, Thomas F. Schilling

Department of Mathematics: Faculty Publications

How does pattern formation occur accurately when confronted with tissue growth and stochastic fluctuations (noise) in gene expression? Dorso-ventral (D-V) patterning of the mandibular arch specifies upper versus lower jaw skeletal elements through a combination of Bone morphogenetic protein (Bmp), Endothelin-1 (Edn1), and Notch signaling, and this system is highly robust. We combine NanoString experiments of early D-V gene expression with live imaging of arch development in zebrafish to construct a computational model of the D-V mandibular patterning network. The model recapitulates published genetic perturbations in arch development. Patterning is most sensitive to changes in Bmp signaling, and the temporal …

Paleoparasitology And Pathoecology In Russia: Investigations And Perspectives, Sergey Slepchenko, Karl Reinhard

Karl Reinhard Publications

Russia, both as the USSR and the Russian federation, provided a source of parasitological theory for decades. A key figure in Russian parasitology was Yevgeny Pavlovsky. He developed the nidus concept, the conceptual basis for the field of pathoecology. He also coined the term “paleoparasitology.” Pathoecology is a foundation concept in archaeological parasitology. Paleoparasitology, as defined by Pavlovsky, is an avenue for understanding of host parasite evolution over very long time periods. These contributions are not fully recognized internationally. Similarly, the long history of Russian paleontological and archaeological investigations are not fully known. Most recently, discoveries from archaeological sites show …

The Paleoepidemiology Of Enterobius Vermicularis (Nemata: Oxyuridae) Among The Loma San Gabriel At La Cueva De Los Muertos Chiquitos (600–800 Ce), Rio Zape Valley, Durango, Mexico, Johnica J. Morrow, Karl Reinhard

Karl Reinhard Publications

One hundred coprolites excavated from La Cueva de los Muertos Chiquitos (600–800 CE) in the Rio Zape Valley of present-day Durango, Mexico, were examined for the presence of helminth eggs utilizing standard archaeoparasitological techniques. Eggs of the human pinworm (Enterobius vermicularis) were recovered from 34 of the 100 coprolites examined. Eggs of parasites were photographed and measured before egg concentration values were calculated for each positive sample. Egg concentration values demonstrated an overdispersed pattern of distribution among the samples (66% uninfected, 25% less than 100 eggs/g, 8% between 100 and 500 eggs/g, and 1% more than 500 eggs/g). …

Taphonomic Considerations On Pinworm Prevalence In Three Ancestral Puebloan Latrines, Morgana Camacho, Alena M. Iñiguez, Karl Reinhard

Karl Reinhard Publications

For archaeological studies it is always necessary to consider taphonomic factors that could have influenced in ancient material preservation. Parasite eggs are usually highly degraded in ancient sites dated from all periods of time and taphonomic factors are mentioned to explain absence and low quantity of eggs found. In this study, we compare parasite egg recovery of three Great House latrines: two from Aztec Ruins (Rooms 219 and 225) and one from Salmon Ruins. We compared through statistical regression the recovery of eggs with the abundance of two classes of decomposers: mites and nematodes. These microorganisms have relation with nematode …

New Evidence Of Ancient Parasitism Among Late Archaic And Ancestral Puebloan Residents Of Chaco Canyon, Rachel E. Paseka, Carrie C. Heitman, Karl Reinhard

Karl Reinhard Publications

Archaeoparasitology provides a unique perspective on the health and habits of ancient cultures through the identification of parasite remains in archaeological materials. We identified eggs of the human whipworm, Trichuris trichiura, in coprolites recovered from Late Archaic (1926–1751 cal. BCE) and Ancestral Puebloan (1039–1163 cal. CE) sites in Chaco Canyon, New Mexico. Our findings represent the earliest record of T. trichiura in North America, the first record of the species from Chaco Canyon, and the first record of a macroparasite from a Late Archaic site (Atlatl Cave) on the Colorado Plateau. T. trichiura is common in the …

Autologous Bone Flap Resorption Years After Subtemporal Craniotomy, John Oladapo Obafunwa, Emily E. Hammerl, David Jaskierny, Livia A. Taylor, Lynette Russell, Karl Reinhard

Karl Reinhard Publications

In Spring of 2012, the partially undressed and skeletonized remains of a homeless adult Hispanic male was found in a fairly open wooded area in Nebraska. The remains showed evidence of extensive pathologies, which included healed traumas and surgeries. Examination of the decedent's medical records revealed that he had a history of kidney and liver problems, alcohol abuse, several traumas including a major head injury that necessitated a craniotomy, and radiological features of neurocysticercosis. The autologous bone flap, which was replaced after the craniotomy, had resorbed significantly away from the edges of the injury. Death was variously attributed to craniocerebral …

Science At Engineer Cantonment, Hugh H. Genoways, Brett C. Ratcliffe, Carl R. Falk, Thomas E. Labedz, Paul R. Picha, John R. Bozell

University of Nebraska State Museum: Mammalogy Papers

Conclusions

It is our contention that Thomas Say, Titian Peale, Edwin James, and their colleagues of the Stephen Long Expedition of 1819–1820 were heavily engaged in scientific research, which took the form of the first biodiversity inventory undertaken in the United States. This accomplishment has been overlooked both by biologists and historians, but it should rank among the most significant accomplishments of the expedition. The results of this inventory continue to inform us today about environmental, faunal, and floral changes along the Missouri River in an area that is known to be an ecotone between the deciduous forests of the …

New Tribal Placement And Review Of Parapucaya Prell And Pucaya Ohaus (Coleoptera, Scarabaeidae, Dynastinae), Aura Paucar-Cabrera, Matthew Robert Moore

Department of Entomology: Faculty Publications

The dynastine scarab genera Parapucaya Prell and Pucaya Ohaus have been historically classified in Pentodontini; however, that tribal classification is not supported under the current tribal circumscriptions. A discussion justifying the transfer of the genera Parapucaya and Pucaya from Pentodontini into Cyclocephalini is presented. This research is based on morphological observations (mandible shape and wing characters among others) and molecular data (genes 28S, COI, and 16S/ND1). A review of both genera is included, providing descriptions, diagnoses, distribution data, illustrations, and keys to species. A revised key to the world genera of Cyclocephalini is also included.

Los escarabajos de los géneros …

Gene Silencing In Tribolium Castaneum As A Tool For The Targeted Identification Of Candidate Rnai Targets In Crop Pests, Eileen Knorr, Elane Fishilevich, Linda Tenbusch, Meghan L. F. Frey, Murugesan Rangasamy, Andre Billion, Sarah E. Worden, Premchand Gandra, Kanika Arora, Wendy Lo, Greg Schulenberg, Pablo Valverde-Garcia, Andreas Vilcinskas, Kenneth E. Narva

Department of Entomology: Faculty Publications

RNAi shows potential as an agricultural technology for insect control, yet, a relatively low number of robust lethal RNAi targets have been demonstrated to control insects of agricultural interest. In the current study, a selection of lethal RNAi target genes from the iBeetle (Tribolium castaneum) screen were used to demonstrate efficacy of orthologous targets in the economically important coleopteran pests Diabrotica virgifera virgifera and Meligethes aeneus. Transcript orthologs of 50 selected genes were analyzed in D. v. virgifera diet-based RNAi bioassays; 21 of these RNAi targets showed mortality and 36 showed growth inhibition. Low dose injection- and …

Clathrin-Dependent Endocytosis Is Associated With Rnai Response In The Western Corn Rootworm, Diabrotica Virgifera Virgifera Leconte, Daniele H. Pinheiro, Ana M. Velez, Elane Fishilevich, Haichuan Wang, Newton P. Carneiro, Arnubio Valencia-Jimenez, Fernando H. Valicente, Kenneth E. Narva, Blair D. Siegfried

Department of Entomology: Faculty Publications

The cellular uptake of dsRNA after dietary exposure is critical for RNAi efficiency; however, the mechanism of its uptake in many insects remains to be understood. In this study, we evaluated the roles of the endocytic pathway genes Clathrin heavy chain (Chc), Clathrin adaptor protein AP50, ADP ribosylation factor-like 1 (Arf72A), Vacuolar H⁺ ATPase 16 kDa subunit (Vha16), and small GTPase Rab7 and putative sid-1-like genes (silA and silC) in RNAi response in western corn rootworm (WCR) using a two-stage dsRNA exposure bioassay. Silencing of Chc, Vha16, and AP50 led …

Rapid And Persistent Rnai Response In Western Corn Rootworm Adults, Ke Wu, Caitlin E. Taylor, Elane Fishilevich, Kenneth E. Narva, Blair Siegfried

Department of Entomology: Faculty Publications

RNA interference (RNAi) has proven effective for controlling pest insects such as western corn rootworm (WCR), Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae). Previous studies have shown that WCR adults display a robust RNAi response to orally-administered double-stranded RNA (dsRNA). However, it is unclear how quickly the response occurs after ingestion or how long RNAi effect lasts after WCR stop ingesting diet containing dsRNA. In the current study, WCR adult females were provided with diet treated with dsRNAs of Laccase 2 and Argonaute 2, two nonessential genes, for 8 days. RNAi response in WCR females commenced as early as 10 h …

Knockdown Of Rna Interference Pathway Genes In Western Corn Rootworm, Diabrotica Virgifera Virgifera, Identifies No Fitness Costs Associated With Argonaute 2 Or Dicer-2, Carolina Camargo, Ke Wu, Elane Fishilevich, Kenneth E. Narva, Blair Siegfried

Department of Entomology: Faculty Publications

The use of transgenic crops that induce silencing of essential genes using double-stranded RNA (dsRNA) through RNA interference (RNAi) in western corn rootworm, Diabrotica virgifera virgifera, is likely to be an important component of new technologies for the control of this important corn pest. Previous studies have demonstrated that the dsRNA response in D. v. virgifera depends on the presence of RNAi pathway genes including Dicer-2 and Argonaute 2, and that down-regulation of these genes limits the lethality of environmental dsRNA. A potential resistance mechanism to lethal dsRNA may involve loss of function of RNAi pathway genes. However, …

Ganganomala Saltini Ratcliffe, Jameson, And Zorn, A Newgenus And Species Ofanomalini (Coleoptera: Scarabaeidae: Rutelinae) From Bangladesh And Nepal, With A Revised Circumscription Of The Tribe, Brett C. Ratcliffe, Mary Liz Jameson, Carsten Zorn

Department of Entomology: Faculty Publications

Ganganomala saltini Ratcliffe, Jameson, and Zorn, a new genus and new species of Anomalini (subtribe Anomalina), is described fromBangladesh and Nepal. To place the new genus within the context of the tribe and subtribe, we provide a key to the four subtribes of world Anomalini, a list of comparative characters and character states for diagnosis and classification of Old World Anomalina, and comparative diagnostic characters for genera of Old World Anomalina. Circumscription, description, diagnosis, and illustrations for the new genus and species are provided. As a result of this research, the Anomalina includes 50 genera and subgenera, and advances our …