Digital Commons Network^™

Studies On E2 Conjugation Enzyme Partners Of Mulan E3 Ubiquitin Ligase, Rebekah J. Fitzpatrick

Honors Undergraduate Theses

Mulan is an E3 ubiquitin ligase embedded in the outer mitochondria membrane. Mulan’s participation in the ubiquitination process is conducted through its cytosol exposed RING finger domain, and its ability to modulate protein ubiquitination makes it a key player in mitochondrial and cellular homeostasis. Mulan is known to be involved in mitochondrial fission, fusion, mitochondrial stress, apoptosis, and Parkin-independent mitophagy. Dysregulation of Mulan in mice has been shown to correlate with human neurodegenerative disorders and heart disease. Accumulation of Mulan is predicted to be responsible for the motor neuron degeneration 2 (mnd2) phenotype in mutant mice through the deregulation of …

Split Deoxyribozyme Probe For Efficient Detection Of Highly Structured Rna Targets, Sheila Raquel Solarez

Honors Undergraduate Theses

Transfer RNAs (tRNAs) are known for their role as adaptors during translation of the genetic information and as regulators for gene expression; uncharged tRNAs regulate global gene expression in response to changes in amino acid pools in the cell. Aminoacylated tRNAs play a role in non-ribosomal peptide bond formation, post-translational protein labeling, modification of phospholipids in the cell membrane, and antibiotic biosynthesis. ^[1] tRNAs have a highly stable structure that can present a challenge for their detection using conventional techniques. ^[2] To enable signal amplification and lower detection limits, a split probe - split deoxyribozyme (sDz or BiDz) probe, which …

Reconstructing The Vocal Capabilities Of Homo Heidelbergensis, A Close Human Ancestor, Austin Blake Stanley

Honors Undergraduate Theses

The discovery of 5,500 Homo heidelbergensis fossil specimens at the Sima de los Huesos archaeological site in Spain has opened up the opportunity for research to be conducted on the vocal capabilities of this species. Previous research has revealed that the range of vowel sounds an individual can produce, known as the vowel space, is directly affected by the dimensions of the vocal tract. The vowel spaces of two hominins, Homo sapiens and Homo neanderthalensis, have been reconstructed through previous research. However, the vowel space of Homo heidelbergensis has not yet been reconstructed. In this research, I aim to …

Hopf Bifurcation Analysis Of Chaotic Chemical Reactor Model, Daniel Mandragona

Honors Undergraduate Theses

Bifurcations in Huang's chaotic chemical reactor system leading from simple dynamics into chaotic regimes are considered. Following the linear stability analysis, the periodic orbit resulting from a Hopf bifurcation of any of the six fixed points is constructed analytically by the method of multiple scales across successively slower time scales, and its stability is then determined by the resulting final secularity condition. Furthermore, we run numerical simulations of our chemical reactor at a particular fixed point of interest, alongside a set of parameter values that forces our system to undergo Hopf bifurcation. These numerical simulations then verify our analysis of …

Gender's Role In Spanish-Language Journalism- An Examination Of How Gender Plays In Hard Vs. Soft News, Victoria R. Elajami

Honors Undergraduate Theses

The inequality of women in the workforce has been an ongoing issue. The 19th amendment granted women the right to vote in 1920. Since then, women have continued to fight for their equality in its entirety. Specifically, in the 21st-century women continue to fight for equality in the work place. Studies have shown that the amount of women in the workplace has exponentially increased. In fact, “they caught up with men in the rates of higher-education graduation, they increased their training and representation in formerly male-dominated professional fields and they entered many previously male dominated occupations” (Vokić, 2017). However the …

Oyster Reef Restoration: Impacts On Infaunal Communities In A Shallow Water Estuary, Katherine P. Harris

Honors Undergraduate Theses

Oyster reefs are important estuarine ecosystems that provide habitat to many species including threatened and endangered wading birds and commercially important fishes and crabs. Infaunal organisms (i.e. small, aquatic animals that burrow in the sediment) are also supported by oyster reef habitats. Infaunal organisms are critical to marine food webs and are consumed by many important species that inhabit coastal estuaries. However, over the past century 85% of shellfish reef habitats have been lost, making restoration of these areas vital. Due to their important role in coastal food webs, infauna is hypothesized to be a strong indicator of habitat productivity …

Investigating The Role Of The Caspase-6 Cleavage Fragment Of Mutant Huntingtin In Huntington Disease Pathogenesis, Jourdan A. Mckinnis

Honors Undergraduate Theses

Huntington disease (HD) is a devastating and fatal neurodegenerative disease. At the moment, no disease modifying therapies are available, with only symptomatic treatment offered to alleviate psychiatric and some types of motor deficits. As a result, many people will continue to suffer and die from this disease. Small molecule therapies have failed to provide benefit in HD, necessitating more complex gene therapy approaches and the identification of less traditional therapeutic targets. A previous study demonstrated that preventing cleavage of the huntingtin (HTT) protein, the protein that when mutated causes HD, by caspase 6 (C6) at amino acid 586 prevents the …

Raman Spectroscopy Of The Skeleton Of The Coral Acropora Cervicornis, Zachary C. Shepard

Honors Undergraduate Theses

Coral reefs are an important element of marine ecosystem that are critical to maintain a healthy environment. Unfortunately, in recent years coral reefs are doing poorly and many in parts of the ocean are simply dying. Therefore, study of coral’s structural response to external loads could answer what will happen with their structures, while they exhibit different types of loading. Therefore, the proposition of using in-situ micro-Raman spectroscopy to study skeletons of Acropora cervicornis was used. Coral skeleton samples I subjected to mechanical loading studied their vibrational properties by exciting the material with 532nm visible light. A uniaxial compressive load …

Can Nox1 Activity Initiate Parkinson's-Like Pathology In An Enteroendocrine Cell Line?, Evan Adler

Honors Undergraduate Theses

Increased attention has been given to the gut lately in a number of conditions, from maintaining health via the use of probiotics to treating Autism Spectrum Disorder. Parkinson's Disease has a history with the gut starting with the Braak hypothesis, in which eminent researcher Heiko Braak observed that the spread of Parkinson's (PD) seemed to occur along either olfactory or enteric neurons prior to reaching the substantia nigra in the midbrain, where the classical disease symptoms become evident. Though this finding was largely ignored at the time, the possibility of a gut origin for PD has received interest lately as …

A Deviance Regulation Theory Intervention To Reduce Alcohol Problems Among First-Year College Students, Angelina V. Leary

Honors Undergraduate Theses

OBJECTIVE: An alcohol-drinking culture exists among first-time-in-college students, where many of these students come to their university relatively inexperienced with alcohol, which may increase alcohol-related consequences. Several interventions exist to combat this campus problem. The current study investigates the use of a Deviance Regulation Theory (DRT) intervention, presented in a web-based manner, to increase alcohol Protective Behavioral Strategies (PBS), such as monitoring drinks, using a designated driver, and drinking water in between alcoholic beverages, among college freshmen. METHOD: College freshmen participants (N = 157) completed web-based surveys examining alcohol behaviors once a week for six weeks. Participants were randomly …

Seeking Help For Children Who Have Experienced Trauma In Venezuela: A Literature Review Of School-Based Interventions And Teacher Recommendations, Maria P. Spinetti Rincon

Honors Undergraduate Theses

The main purpose of this research synthesis was to determine recommendations that promote the development of a trauma-informed approach in Venezuelan schools to address the traumatic effects of political unrest in young children through a systematic review of existent intervention programs. After conducting an initial search, four studies were identified that studied the effectiveness of an intervention program in schools targeting trauma in children younger than 14 years old.

Results showed that there were four categories that all studies incorporated. All intervention programs used an assessment tool to determine PTSD presence and symptomatology, as well as other domains that could …

Cross-Modal Distraction On Simultaneous Translation: Language Interference In Spanish-English Bilinguals, Violet A. Young

Honors Undergraduate Theses

Bilingualism has been studied extensively in multiple disciplines, yet we are still trying to figure out how exactly bilinguals think. A bilingual advantage has been observed in various experimental studies, but also has not been observed in many other studies. A bilingual advantage has been shown in tasks using selective attention. These tasks study the effects of language interference, where two types of interference are observed: interlingual (between-languages) and intralingual (within one language). This study examined language interference in Spanish-English bilinguals, using an auditory-visual simultaneous translation experimental setup. 16 college English monolinguals and 17 college Spanish-English bilinguals were tested. The …

Social Anxiety And Subtypes Of Empathy: The Moderating Influence Of Biological Sex, Samantha K. Berg

Honors Undergraduate Theses

Only a few studies have examined relationships between social anxiety and subtypes of empathy. Findings are mixed. The present study examined social anxiety severity on a continuum and how it related to affective and cognitive empathy in 684 nonpsychiatric adults (77% female). Participants completed an online battery of measures that included: a self-report measure of social anxiety severity (Fear of Negative Evaluation), a self-report measure with subscales for affective and cognitive empathy (Interpersonal Reactivity Index), and a behavioral measure of cognitive empathy (Reading the Mind in the Eyes Task; MIE). After statistically covarying for general anxiety severity, biological sex moderated …

Soil Organic Carbon And Mineralization Rates At The Woolsey Wet Prairie Mitigation Site In Fayetteville, Arkansas, Zachary Tipton, Lisa S. Wood, Mary Savin, Benjamin R. Runkle

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Atmospheric carbon dioxide (CO₂) levels are rapidly increasing, surpassing 400 ppm in 2013 from a pre-industrial revolution level of around 280 ppm. Researchers have been looking at methods to reduce CO₂ levels in the atmosphere, including promoting carbon sequestration in soils. Carbon sequestration is the process where CO₂ is naturally or artificially transferred out of the atmosphere and stored in the ocean, plant biomass, soils, and geologic formations. Seemingly contradictory to the notion of carbon sequestration is the use of fire as a management treatment for the restoration of native prairie grass ecosystems. Fire combusts plant …

Reproductive Rights And Justice Advocacy In Central Florida: Who Is Represented?, Mary Hager

Electronic Theses and Dissertations

This thesis examines the racial understanding and social relationships of Planned Parenthood of Southwest and Central Florida staff and volunteers. As well, this research explores how Planned Parenthood supporters organize and promote diverse advocacy work to promote reproductive justice in social media and volunteer education. Planned Parenthood has been at the forefront of reproductive rights campaigns for over a century, and their work has often tied into contemporary feminist and political issues. Of note, feminists of color have called for a shift from advocacy for "reproductive choice" to "reproductive justice" as a way to identify the needs and predicaments of …

Polyelectrolyte Complexes Based On Poly(Acrylic Acid): Mechanics And Applications, Xiaoyan Lu

Electronic Theses and Dissertations

Poly(acrylic acid) (PAA) is a weak polyelectrolyte presenting negative charge at basic condition when the carboxylic group loses a proton. These carboxylate group can interact with polycations and metal ions to form stable polyelectrolyte complexes (PECs), leading to tunable properties and multifunctional nanoscale structures through chemical reactions. This research focuses on nanofiber and nanoparticle fabricated by PAA-based PECs. We demonstrated the effect of ferric ion concentration on the mechanical properties of PAA-based single naonofiber by using dark field microscopy imaging and persistence length analysis. The application of PAA-based nanofiber mats loaded with MnO2 for supercapacitors was also explored. As a …

College Student Attitudes Towards Free Speech And Expression, David Oglethorpe

Electronic Theses and Dissertations

Throughout its storied history, higher education in the United States has dealt with the challenges of free speech. From Harvard's 1766 'bad butter riot' to hateful speech directed towards students by non-university community members, balancing the need for free speech with maintaining a welcoming and inclusive campus environment stands as an immense test for higher education (Papandrea, 2017). Idealism and ethicality aside, lost in the academic debate over liberty and protections are the views of those who should best help shine a light on such a divisive issue: students. This dissertation creates a quantitative path to understanding those very viewpoints. …

Coming To A Full Stop: An Investigation Of Menstrual Period Stigmas In College Students, Mary Jane O'Toole

Electronic Theses and Dissertations

The focus of this study was to understand perceptions college students have regarding menstruation and if viewing a normalized media message may influence these perceptions. A treatment group of participants was randomly assigned to view an episode of the hit family sitcom Blackish that focused on menstruation and then answer survey questions. A control group of participants only answered the survey questions. A mixed methods analysis revealed three primary conclusions. First, these results contradict existing research in that the college students surveyed generally do not hold negative perceptions that may stigmatize menstruation. Second, the treatment that viewed the normalized media …

Learning Spaces Are Wac: Investigating How Classroom Space Design Influences Student Disciplinary Identities, Landon Berry

Electronic Theses and Dissertations

This dissertation used classroom observations, movement mapping, instructor interviews, and student focus groups to examine the ways in which both instructors and students navigated the classroom spaces they were assigned in upper-level, discipline-specific courses. By focusing on three diverse disciplines (writing and rhetoric, education, and chemistry), this dissertation makes arguments about how the design of classroom spaces (as well as the tools that are housed therein) support, facilitate, and detract from a student's ability to develop a disciplinary identity, which is defined here as the social and linguistic construction of a practitioner of a discipline that is shaped by the …

Agricultural Information Needs And Food Access In The Stann Creek District Of Belize, Sam E. Harris, Donna L. Graham

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

The purpose of this study was to describe agricultural information sources available to farmers and to describe food access and availability for the people of Dangriga, Stann Creek, Belize. This study used descriptive survey research methods with convenience sampling of the general public (n=22) and of farmers (n = 38) in the summer of 2017. Farmers use a variety of agricultural information sources with the extension service cited most often, followed by friends and fellow farmers. Weather, lack of information, pests, and inadequate access to capital were of primary concern for farmers. Face-to-face meetings were used most often by extension …

Animals Of The Cloud Forest: Isotopic Variation Of Archaeological Faunal Remains From Kuelap, Peru, Samantha Michell

Electronic Theses and Dissertations

Stable isotopic analyses of faunal remains are used as a proxy for reconstructing the ancient Chachapoya dietary environment of the northeastern highlands in Peru. Archaeologists have excavated animal remains from refuse piles at the monumental center of Kuelap (AD 900-1535). This archaeological site is located at 3000 meters above sea level (m.a.s.l.), where C3 plants dominate the region. The study presented here is one of the few in the Central Andes that uses faunal remains to develop local isotopic baselines, reconstruct resource exploitation, and provide insight into dietary variation. Bone collagen stable carbon (δ13C) and nitrogen (δ15N) isotopes are used …

Development Of A Non-Human Primate Model For Staphylococcus Aureus Nasal Carriage, Amanda Lasseter

Electronic Theses and Dissertations

Staphylococcus aureus nasal carriage (SANC) is largely asymptomatic, but presents a risk of autoinfection and dissemination to new immunocompromised hosts. SA disease states range from mild cutaneous infections to life-threatening bacteremia. Historically utilized rodent models do not naturally carry SA in the nose, are insufficient in longitudinal SANC experimentation, and lack immune factors that are vital in human clearance of SA. The nasal passages of non-human primates are similar anatomically and histologically, and reproductive mucosal studies have shown similar immune responses to pathogens and human-relevant microbial profiles. Seventeen captive pigtailed macaques (Macaca nemestrina) were found to naturally carry SA in …

Machine Learning Methods For Flow Cytometry Analysis And Visualization, Emily Sassano

Electronic Theses and Dissertations

Flow cytometry is a popular analytical cell-biology instrument that uses specific wavelengths of light to profile heterogeneous populations of cells at the individual level. Current cytometers have the capability of analyzing up to 20 parameters on over a million cells, but despite the complexity of these datasets, a typical workflow relies on subjective labor-intensive manual sequential analysis. The research presented in this dissertation provides two machine learning methods to increase the objectivity, efficiency, and discovery in flow cytometry data analysis. The first, a supervised learning method, utilizes previously analyzed data to evaluate new flow cytometry files containing similar parameters. The …

The Evolution Of Peristenus (Hymenoptera: Braconidae): Taxonomy, Phylogenetics And Ecological Speciation, Yuanmeng Zhang

Electronic Theses and Dissertations

Parasitoid wasps are ecologically and economically important as biological control agents. However, little is known about the diversity, distribution and biology of most hymenopteran parasitoids due to their small size, morphological conservatism, and complex life styles. The focus of my PhD research was to investigate the evolution and speciation of euphorine braconid wasps, using a combination of multilocus phylogenetics and population genomic techniques combined with traditional taxonomy. The three data chapters of my dissertation are divided into different taxonomic ranks of euphorine braconids, focusing on genera, species, and populations. For chapter 2, I built a multilocus phylogeny of the tribe …

Countering The Narrative: Exploring The Relationships Among Wellness, Resilience, And Empowerment Within Black Men Who Have Sex With Men Living With Hiv (Bmsm+), Nevin Heard

Electronic Theses and Dissertations

The HIV epidemic continues to disproportionately impact marginalized populations, where one in two Black men who have sex with men (BMSM) will be diagnosed with HIV in their lifetime. The lack of research regarding the wellness of Black men who have sex with men living with HIV (BMSM+) inhibits understanding, which could hinder professions missioned with bettering the wellness of BMSM+. Consequently, the purpose of this study was to investigate if resilience and empowerment predict BMSM+'s wellness. However, a lack of internal consistency among the empowerment measurement required the researcher to remove the scale and adjust the scope of the …

Revisiting The Postclassic Burials At Lamanai, Belize: A Second Look At The Unique Ventrally Placed, Legs Flexed Burials., Victoria Izzo

Electronic Theses and Dissertations

Analysis of unique mortuary patterns is often used to evaluate the social lives of the deceased and also those of the living who placed them there. The Ventrally Placed, Legs Flexed (VPLF) burials at the site of Lamanai in Belize, dating to the Postclassic period (1000 - 1544), have been recorded as a Maya mortuary pattern since the late 1970's. While many researchers have analyzed these skeletal remains, comprehensive and cumulative individual analysis of the VPLF individuals from Lamanai has not been conducted. In this study, I will argue that the VPLF individuals in this study were local to Lamanai, …

Testing The Capability Of Close-Range Photogrammetry To Document Outdoor Forensic Scenes With Skeletal Remains Using Mock Scenarios, Kevin Gidusko

Electronic Theses and Dissertations

More rigorous methodological protocols are needed to document outdoor forensic scenes containing skeletal remains. However, law enforcement protocols rarely provide specific guidelines for processing these scenes. Regardless, the need to preserve contextual information at crime scenes is of paramount importance and it is worth exploring new technological applications that will allow for better documentation. Close-range photogrammetry (CRP) is one option for outdoor scene documentation, more prominently utilized in archaeological contexts, that may provide forensic archaeologists with a tool to better document these scenarios via 3D modeling. To test the efficacy of CRP as documentation tool three mock scenarios representing common …

Embryonic Stem Cell Derived Exosomes Enhance Cardiac Stem Cell Differentiation Into Heart Cells, Jamillah Hammond

Electronic Theses and Dissertations

Transplantation of embryonic stem (ES) cells into the ischemic and infarcted heart has proven to repopulate cardiac cell populations, attenuate structural cardiac remodeling, and rescue cardiac function. Unfortunately, the pluripotency of ES cells increases risk of teratoma formation in vivo. Exosomes, smaller in comparison to ES cells, are cell free carriers of miRNA, proteins, and lipids, and do not suggest risk of teratoma formation. Exosomes have been proposed to mediate and attenuate regeneration following myocardial infarction (MI), however, the role of exosomes derived from ES cells (ES-Exo) in activating resident cardiac stem cells (CSCs) to undergo cardiac differentiation is not …

Childhood Experiences And Domestic Violence: The Role That Interpartner Violence Plays In Relationship Functioning And Parenting Behaviors In Adulthood., J'Nelle Stephenson

Electronic Theses and Dissertations

It is estimated that approximately 25% of women and 7.6% of men report experiencing violence from their romantic partner during their lifetime. Additionally, in households where interpartner violence occurs, there are between 3.3 and 10 million children in the United States alone. It is important to understand the cycle of domestic violence that can occur, as it can inform us about expected outcomes across time. This study examined mothers' childhood exposure to their own parents' domestic violence. It was hypothesized that childhood exposure to domestic violence would be related to unhealthy intimate relationships and to the development of maladaptive parenting …

Gender And Conflict In Long-Term Romantic Relationships, Emily Hamlin

Electronic Theses and Dissertations

Conflict is inevitable, but not inherently negative. All relationships face conflict with some frequency and long-term romantic partnerships are no exception. This exploratory qualitative study investigated conflict types, approaches, and affective components using digital conflict journals. Participants logged their relational conflict for one month in order to gain insight into the communication practices and conflict perceptions of couples. A conflict cycle and sensemaking framework was applied to identify patterns and best practices.