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Watered Down: The Challenges Of Managing Water Resources In Montana, Beau E. Baker

Graduate Student Theses, Dissertations, & Professional Papers

Like much of the American West, Montana sits in the cross hairs of climate change. State drought resiliency projects and cooperative watershed management are on the rise in the face of decreased snowpack, early runoff, precipitation variability and lower seasonal stream flows. Population growth, land use practices, recreation and tourism all contribute to pressures on state water supplies.

Montana is faced with the arrival of invasive species that threaten the ecological health of its lakes, rivers and streams. State budget constraints and depressed agency capacity are hurting our ability to fend off these threats. There’s a lack of public education …

Breaking Chains Of Oppression: Popular Culture And The Plundering Of Blackness, Corina Sacajawea Ambrose

Graduate Student Theses, Dissertations, & Professional Papers

This thesis focuses on the ways in which white supremacy created mass incarceration, specifically mass incarceration of black individuals, and how this continues to perpetuate a racial caste system in the United States. First, I examine contemporary novelist Colson Whitehead‘s The Underground Railroad to provide a historical background of white supremacy and slavery. Then, I argue that pop culture is one area in which artists are focused on the abolition of the prison-industrial complex and ending mass incarceration. Finally, I focus on JAY-Z‘s music video “The Story of O.J.“ and Beyoncé‘s visual album Lemonade and her 2018 Coachella performance to …

Weed Control As A Collective Action Problem: Quantifying Group Effects On Individual Behavior, And Clarifying The Theoretical Frame, Alice Lubeck

Graduate Student Theses, Dissertations, & Professional Papers

Weeds reduce the biodiversity and productivity of agricultural systems, and are a problem both around the world, and in Montana. Weeds are challenging to control because their effective dispersal mechanisms enable cross-boundary colonization, and so managers must engage diverse groups of private landowners. Researchers have recognized weed control is a collective action problem, but there is little research quantifying the role of collective factors on an individual’s decision to control. To fully understand the motivations behind independent weed control, I initiated a study to quantitatively assess different types of landowners and their weed control behaviors, the relationship between collective interest …

The Effects Of Common Methods Of Soft Tissue Removal On Skeletal Remains: A Comparative Analysis, Emily Silverman

Graduate Student Theses, Dissertations, & Professional Papers

The removal of soft tissue from skeletal remains is a process familiar to a wide array of scientific fields and the methods used to perform it are likewise numerous yet inconsistent. In forensic investigations and crime labs across the country, there lacks a standardization for this process. This lack of standardization pairs with a distinct lack of literature on the potential benefits and risks associated with each method as well as basic information on the proper amount of additives, temperatures, or time estimations. In a forensic context, human remains may be the only evidence available, which makes any damage or …

Interleukin-6 And Exercise; Early Evidence Of A Novel Myokine, Brendan Hogg Mr.

Graduate Student Theses, Dissertations, & Professional Papers

Interleukin-6 (IL-6) was first identified as a pleiotropic cytokine, with a host of body-wide functions. Cytokine functions are characterized by chronically elevated levels within various inflammatory states. In this regard, IL-6 is largely associated with the acute phase response to many stimuli and performs specific actions when produced from certain cell types. Accrued evidence indicates IL-6 release from skeletal muscle often includes myokine functions. Novel myokine functions are adaptive in nature, and as compared to inflammatory/cytokine roles, exhibit a transient time course. Following exercise plasma IL-6 peaks and returns to resting levels within 1-2 hours. In contrast, IL-6 is observed …

Extending The Fossil Record Of The Polytrichaceae (Bryophyta): Insights From The Early Cretaceous Of Vancouver Island, Canada, Alexander C. Bippus

Cal Poly Humboldt theses and projects

Diverse in modern ecosystems, mosses are dramatically underrepresented in the fossil record. Furthermore, most pre-Cenozoic mosses are known only from compression fossils, which lack detailed anatomical information. Lower Cretaceous deposits at Apple Bay (Vancouver Island, British Columbia, Canada) contain a diverse anatomically preserved flora that includes numerous bryophytes, many of which have yet to be characterized. Among them is a polytrichaceous moss that is described here as Meantoinea alophosioides gen. et sp. nov. Meantoinea alophosioides represents the first occurrence of gemma cups in a fossil moss and is the oldest unequivocal record of Polytrichaceae, providing a hard minimum age for …

Fall Risk Behaviors And Intrinsic Risk Factors For Falls In Indigenous And Non-Indigenous Rural Older Adults, Andre Gallegos Bouweraerts

Cal Poly Humboldt theses and projects

The purpose of this study was to examine fall risk behaviors and intrinsic risk factors for falls in rural dwelling indigenous and non-indigenous older adults in California. Methods: Participants included 157 adults 60+ years of age (89 indigenous and 68 non- indigenous) and were recruited from advertisements in local newspapers, senior centers, and health clinics in Humboldt and Del Norte Counties. Fall risk behaviors and intrinsic risk factors for falls were identified through administration of a demographic, fall risk, and physical activity questionnaire as well as the chair stand, functional reach, timed up and go, four-meter walk, and modified clinical …

Evaluating Current And Future Range Limits Of An Endangered, Keystone Rodent (Dipodomys Ingens), Ivy V. Widick

Cal Poly Humboldt theses and projects

Climate is often considered the single most important factor limiting species’ ranges. Other factors, such as biotic interactions, are often assumed to be included via abiotic proxies. However, differential responses to climate change may decouple these relationships or lead to adaptation to novel environments. Accounting for competition and local adaptation should more accurately describe environmental factors influencing current distributions and increase the predictive accuracy of future distributions. Modeling the endangered giant kangaroo rat (Dipodomys ingens) is an excellent application of these model improvements, as the species range consists of geographically and genetically isolated populations experiencing disparate climatic change. …

Anchoring Female Millennial Students In An It Career Path: The Class Anchor Model, Pam Rowland, Cherie Noteboom

Research & Publications

Goals and desires are strong incentives for careers and life choices. When goals and desires are not met, change often occurs. Women are leaving the IT profession two times faster than men and often within the first twelve years of employment. Women are also underrepresented in the IT profession with only 25 percent of the current jobs being held by women. This study examines how organizations can retain females in IT professions through motivational anchors. While there are research studies that have investigated the gender gap, there is a need to investigate female millennial students’ relationship to IT through their …

Structural Characterization Of The Human C-Myc Quadruplex And Mechanistic Evaluation Of Antitumor Agents: Anthracenyl Isoxazole Amides And Quinolinediones, Sascha Christian Lamotte Stump

Graduate Student Theses, Dissertations, & Professional Papers

Cancer is a disease characterized by the abnormal proliferation of cells in the body. For many forms of cancer, there remain only limited and often ineffective treatment options available. Treatment of cancer is problematic for several reasons that include the difficulty in establishing molecular targets, finding interventions that cause selective toxicity to cancer cells, and the uniform capability of cancer cells to evade apoptosis. To address this, new strategies must be employed that take advantage of novel mechanisms of action to develop better therapies. The goal of this research is to aid in this effort through the study of emerging …

Global Change And Whitebark Pine: Restoration, Refugia, And Alpine Treelines, Colin Taylor Maher

Graduate Student Theses, Dissertations, & Professional Papers

Whitebark pine is a major component of subalpine forests in western North America. The species occupies harsh high-mountain sites up to treeline, where it is often the dominant species. The species is ecologically important but is also a valuable species for studying the dynamics of alpine treelines. However, whitebark pine has experienced significant mortality in recent decades from mountain pine beetle outbreaks and white pine blister rust. This kind of rapid environmental change presents significant challenges to our understanding and management of the dynamics of ecological communities. On one hand, the effects of climate change on forest ecosystems could provide …

An Interdisciplinary Approach To The Target Elucidation Of Novel Antibiotic 31g12, Larissa A. Walker

Graduate Student Theses, Dissertations, & Professional Papers

Staphylococcus aureus is a Gram-positive bacterial pathogen responsible for nosocomial and community-acquired infections that can quickly acquire antibiotic resistance. We have identified a novel triazole antimicrobial 31G12 based on the natural product core of nonactin isolated from the fermentation of Streptomyces griseus, that is active against many Gram-positive bacteria as well as antibiotic resistant methicillin-resistant S. aureus and vancomycin-resistant Enterococcus. The synthesis and characterization indicate that 31G12 exists as a mixture of two rotamers at room temperature and displays bacteriostatic activity against S. aureus with moderate mammalian cell toxicity. We have currently identified potential protein targets of 31G12 in …

Exploring The Ligand-Dependent Conformational Ensemble Of Pparγ: More Than A Mousetrap, Ian Michael Chrisman

Graduate Student Theses, Dissertations, & Professional Papers

The nuclear receptor PPARγ is a lipid-dependent transcription factor which regulates many pathways in lipid metabolism. Due to functions in adipogenesis, glucose uptake and insulin sensitivity it has been a target of type II diabetes mellitus therapeutics since the 1990s. Due to a poorly understood anti-inflammatory pathway it is also an emerging target in neurodegenerative disease. Despite extensive research the majority of the structural mechanism underlying the regulation of this proteins’ function is poorly understood. It is often thought that nuclear receptors function in a simple two-state model with defined on and off states. This model only considers one region …

Leaving More Than Footprints: Impacts Of Tourism And Perceptions Of Change In Chilean Patagonia, Heidi Blair

Graduate Student Theses, Dissertations, & Professional Papers

Visitation to protected areas is on the rise globally, and the Aysén region of Chilean Patagonia is part of that trend. Within Aysén, Cerro Castillo National Reserve is one of the protected areas that has seen the largest increase in visitation in recent years. Protected area tourism catalyzes changes in surrounding communities, and it is important to understand resident perceptions of these changes in order to inform management planning and to implement effective, equitable conservation policy. In this study, I use qualitative research methods to investigate local residents’ perceptions of social, economic, and ecological changes spurred by or related to …

Honors @ Georgia Southern, University Honors Program Students And Staff, Georgia Southern University

Honors College: News & Publications (2008-2020)

• Bob Mikell and Darin Van Tassell
• Honors Students ‘Fight Dirty’
• Honors Travels to D.C.
• 2017-2018 Graduates

The Effects Of Physical Activity On Sleep Patterns In Children With Developmental Disabilities, Rebecca Griffith

Cal Poly Humboldt theses and projects

Individuals with developmental disabilities should be engaged in 150 minutes of physical activity every week to experience health benefits including an increase in duration and quality of sleep. Individuals with developmental disabilities who get quality, regular sleep have a lower frequency of challenging behaviors. One way to increase physical activity engagement among individuals with disabilities is to engage their family in physical activity together. To analyze the effects of engaging in a family centered physical activity program on the duration and quality of sleep in a young adult with Down syndrome. Method: The participant engaged in the Humboldt State Fitfam …

Alteration Of Metabolite Profiling By Cold Atmospheric Plasma Treatment In Human Myeloma Cells, Dehui Xu, Yujing Xu, Ning Ning, Qingjie Cui, Zhijie Liu, Xiaohua Wang, Dingxin Liu, Hailan Chen, Michael G. Kong

Bioelectrics Publications

BACKGROUND: Despite new progress of chemotherapy in multiple myeloma (MM) clinical treatment, MM is still a refractory disease and new technology is needed to improve the outcomes and prolong the survival. Cold atmospheric plasma is a rapidly developed technology in recent years, which has been widely applied in biomedicine. Although plasma could efficiently inactivate various tumor cells, the effects of plasma on tumor cell metabolism have not been studied yet.

METHODS: In this study, we investigated the metabolite profiling of He plasma treatment on myeloma tumor cells by gas-chromatography time-of-flight (GC-TOF) mass-spectrometry. Meanwhile, by bioinformatic analysis such as GO and …

Nano-Pulse Stimulation Ablates Orthotopic Rat Hepatocellular Carcinoma And Induces Innate And Adaptive Memory Immune Mechanisms That Prevent Recurrence, Brittany P. Lassiter, Siqi Guo, Stephen J. Beebe

Bioelectrics Publications

Nano-pulse stimulation (NPS), previously called nsPEFs, induced a vaccine-like effect after ablation of orthotopic N1-S1 hepatocellular carcinoma (HCC), protecting rats from subsequent challenges with N1-S1 cells. To determine immunity, immune cell phenotypes were analyzed in naïve, treated and protected rats. NPS provides a positive, post-ablation immuno-therapeutic outcome by alleviating immunosuppressive T regulatory cells (Treg) in the tumor microenvironment (TME), allowing dendritic cell influx and inducing dynamic changes in natural killer cells (NKs), NKT-cells and T-lymphocytes in blood, spleen and liver. NPS induced specific increases in NKs and NKT-cells expressing CD8 and activation receptors CD314-NKG2D and CD161 (NK1.1) in the TME …

Cell Fragmentation And Permeabilization By A 1 Ns Pulse Driven Triple-Point Electrode, Enbo Yang, Joy Li, Michael Cho, Shu Xiao

Bioelectrics Publications

Ultrashort electric pulses (ns-ps) are useful in gaining understanding as to how pulsed electric fields act upon biological cells, but the electric field intensity to induce biological responses is typically higher than longer pulses and therefore a high voltage ultrashort pulse generator is required. To deliver 1 ns pulses with sufficient electric field but at a relatively low voltage, we used a glass-encapsulated tungsten wire triple-point electrode (TPE) at the interface among glass, tungsten wire, and water when it is immersed in water. A high electric field (2MV/cm) can be created when pulses are applied. However, such a high electric …

Tumor Cell Death After Electrotransfer Of Plasmid Dna Is Associated With Cytosolic Dna Sensor Upregulation, Katarina Znidar, Masa Bosnjak, Nina Semenova, Olga N. Pakhomova, Loree Heller, Maja Cemazar

Bioelectrics Publications

Cytosolic DNA sensors are a subgroup of pattern recognition receptors (PRRs) and are activated by the abnormal presence of the DNA in the cytosol. Their activation leads to the upregulation of pro-inflammatory cytokines and chemokines and can also induce cell death. The presence of cytosolic DNA sensors and inflammatory cytokines in TS/A murine mammary adenocarcinoma and WEHI 164 fibrosarcoma cells was demonstrated using real time reverse transcription polymerase chain reaction (RT-PCR), western blotting and enzyme-linked immunosorbent assay (ELISA). After electrotransfer of plasmid DNA (pDNA) using two pulse protocols, the upregulation of DNA-depended activator of interferon regulatory factor or Z-DNA binding …

Cold Atmospheric Plasma As A Potential Tool For Multiple Myeloma Treatment, Dehui Xu, Yujing Xu, Qingjie Cui, Dingxin Liu, Zhijie Liu, Xiaohua Wang, Yanjie Yang, Niaojuan Feng, Rong Liang, Hailan Chen, Kai Ye, Michael G. Kong

Bioelectrics Publications

Multiple myeloma (MM) is a fatal and incurable hematological malignancy thus new therapy need to be developed. Cold atmospheric plasma, a new technology that could generate various active species, could efficiently induce various tumor cells apoptosis. More details about the interaction of plasma and tumor cells need to be addressed before the application of gas plasma in clinical cancer treatment. In this study, we demonstrate that He+O₂ plasma could efficiently induce myeloma cell apoptosis through the activation of CD95 and downstream caspase cascades. Extracellular and intracellular reactive oxygen species (ROS) accumulation is essential for CD95-mediated cell apoptosis in response …

Upregulation Of Dna Sensors In B16.F10 Melanoma Spheroid Cells After Electrotransfer Of Pdna, Katarina Znidar, Masa Bosnjak, Tanja Jesenko, Loree C. Heller, Maja Cemazar

Bioelectrics Publications

Increased expression of cytosolic DNA sensors, a category of pattern recognition receptor, after control plasmid DNA electrotransfer was observed in our previous studies on B16.F10 murine melanoma cells. This expression was correlated with the upregulation of proinflammatory cytokines and chemokines and was associated with cell death. Here, we expanded our research to include the influence of features of cells in a 3-dimensional environment, which better represents the tumors’ organization in vivo. Our results show that lower number of cells were transfected in spheroids compared to 2-dimensional cultures, that growth was delayed after electroporation alone or after electrotransfer of plasmid …

Excitation And Injury Of Adult Ventricular Cardiomyocytes By Nano- To Millisecond Electric Shocks, Iurii Semenov, Sergey Grigoryev, Johanna U. Neuber, Christian W. Zemlin, Olga N. Pakhomova, Maura Casciola, Andrei G. Pakhomov

Bioelectrics Publications

Intense electric shocks of nanosecond (ns) duration can become a new modality for more efficient but safer defibrillation. We extended strength-duration curves for excitation of cardiomyocytes down to 200 ns, and compared electroporative damage by proportionally more intense shocks of different duration. Enzymatically isolated murine, rabbit, and swine adult ventricular cardiomyocytes (VCM) were loaded with a Ca²⁺ indicator Fluo-4 or Fluo-5N and subjected to shocks of increasing amplitude until a Ca²⁺ transient was optically detected. Then, the voltage was increased 5-fold, and the electric cell injury was quantified by the uptake of a membrane permeability marker dye, propidium …

Nano-Pulse Stimulation For The Treatment Of Pancreatic Cancer And The Changes In Immune Profile, Sigi Guo, Niculina I. Burcus, James Hornef, Yu Jing, Chunqi Jiang, Richard Heller, Stephen J. Beebe

Bioelectrics Publications

A Pancreatic cancer is a notorious malignant neoplasm with an extremely poor prognosis. Current standard of care is rarely effective against late-stage pancreatic cancer. In this study, we assessed nanopulse stimulation (NPS) as a local treatment for pancreatic cancer in a syngeneic mouse Pan02 pancreatic cancer model and characterized corresponding changes in the immune profile. A single NPS treatment either achieved complete tumor regression or prolonged overall survival in animals with partial tumor regression. While this is very encouraging, we also explored if this local ablation effect could also result in immune stimulation, as was observed when NPS led to …

Enhanced Electric Pulse Technology For The Ablation Of Pancreatic Cancer, Siqi Guo, Niculina I. Burcus, Chelsea M. Edelblute, James Hornef, Chunqi Jiang, Karl Schoenbach, Richard Heller, Stephen J. Beebe

Bioelectrics Publications

Electric pulse based technology has been developed and studied as a non-thermal ablation method for local control of pancreatic cancer. Irreversible electroporation (IRE) has shown a significant survival benefit for local advanced pancreatic cancer in clinical trials. However, incomplete ablation with local recurrence and major complications limit the potential of this new technology. We have developed an integrated moderate heating electric pulse delivery system which consists of controllable tumor heating, multi-parameter monitoring and electric pulse delivery. The impedance of tumor is greatly decreased after moderate heating at 42°C for 1–2 min, which does not cause any cell death. Moderate heating …

Assessment Of Peripheral Bdnf Levels Over 30 Days, Sally Hang

Cal Poly Humboldt theses and projects

Brain health, and the benefits of exercise have been linked to the biological signaling molecule called brain-derived neurotrophic factor (BDNF). Animal and human studies have provided some support for physical exercise as a mechanism for increasing BDNF levels. However, results have been inconsistent, which may be attributed in part to incomplete information about normal variation in circulating peripheral BDNF levels. This investigation examined capillary-drawn whole blood samples from nine healthy adult participants over 30 days with the goal of documenting variability in resting BDNF levels and changes that may be attributed to physical exercise. It was hypothesized that BDNF concentrations …

Apparent Survival Of Snowy Plovers Varies Seasonally, Nora C. Papian

Cal Poly Humboldt theses and projects

Survival is an important vital rate that contributes to population viability, but is infrequently monitored and studied, especially compared to productivity. Furthermore, factors that limit survival and the relative effect on survival are often unknown. I used mark-resight observations of a small (~350) population of threatened Snowy Plovers (Charadrius nivosus nivosus) to quantify seasonal and annual variation in survival and movement between three coastal locations across ~70 km in Humboldt County, California. The return of individuals to non-breeding flocks at three locations was high between years (75-81%). Movement between three locations varied greatly, although most (n = 137) …

Stress And Frailty In Medieval Prussia: Interpretations From Skeletal Remains At Bezławki, Katherine E. Gaddis

Cal Poly Humboldt theses and projects

Health is routinely studied in living populations using quantifiable measurements such as allostatic load and frailty. In recent years, particularly since the introduction of the osteological paradox, there has been increased interest among bioarchaeologists in how these concepts can be applied to the study of health in past populations. Although health is not directly observable in skeletal remains, assessment of frailty can be useful for understanding the implications of long-term exposures to stress on well-being and mortality. This study builds upon past research in this area by incorporating commonly observed indicators of physiological stress, such as dental disease and osteoarthritis, …

Negatively Correlated Abundance Suggests Competition Between Red Abalone (Haliotis Rufescens) And Red Sea Urchins (Mesocentrotus Franciscanus) Inside And Outside Established Mpas Closed To Commercial Sea Urchin Harvest In Northern California, Johnathan Centoni

Cal Poly Humboldt theses and projects

Red abalone and sea urchins are both important herbivores that potentially compete with each other for resources like food and space along the California coast. Red abalone supported a socioeconomically important recreational fishery during this study (which was closed in 2018) and red sea urchins support an important commercial fishery. Both red sea urchins and red abalone feed on the same macroalgae (including Pterygophora californica, Laminaria setchellii, Stephanocystis osmundacea, Costaria costata, Alaria marginata, Nereocystis leutkeana), and a low abundance of this food source during the period of this project may have created a highly competitive environment for urchins and …

The Interactive Effects Of Ocean Acidification, Food Availability, And Source Location On The Growth And Physiology Of The California Mussel, Tharadet Daniel Man

Cal Poly Humboldt theses and projects

Research shows ocean acidification (OA) can have largely negative impacts on marine organisms and ecosystems. Prior laboratory studies show that shelled marine invertebrates (e.g., molluscs) exhibit reduced growth rates and weaker shells when experiencing OA-related stress. However, populations of the critical intertidal mussel species, Mytilus californianus, which experience naturally acidic water due to upwelling in certain parts of Northern California have been observed to have relatively stronger and thicker shells and higher growth rates than those that experience less frequent exposure to upwelling. To address the discrepancies between negative effects of OA exposure in the laboratory and seemingly positive …