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Characterization Of A Protozoan Phosducin-Like Protein-3 (Phlp-3) Reveals Conserved Redox Activity, Rachel L. Kooistra, Robin David, Ana C. Ruiz, Kyle J. Haselton, Kaitlyn Kiernan, Andrew M. Blagborough, Ligin Solamen, Kenneth W. Olsen, Catherine Putonti, Stefan M. Kanzok

Biology: Faculty Publications and Other Works

We recently identified three novel thioredoxin-like genes in the genome of the protozoan parasite Plasmodium that belong to the Phosducin-like family of proteins (PhLP). PhLPs are small cytosolic proteins hypothesized to function in G-protein signaling and protein folding. Although PhLPs are highly conserved in eukaryotes from yeast to mammals, only a few representatives have been experimentally characterized to date. In addition, while PhLPs contain a thioredoxin domain, they lack a CXXC motif, a strong indicator for redox activity, and it is unclear whether members of the PhLP family are enzymatically active. Here, we describe PbPhLP-3 as the first phosducin-like protein …

Characterization Of A Protozoan Phosducin-Like Protein-3 (Phlp-3) Reveals Conserved Redox Activity, Rachel L. Kooistra, Robin David, Ana C. Ruiz, Sean W. Powers, Kyle J. Haselton, Kaitlyn Kiernan, Andrew M. Blagborough, Ligin Solamen, Kenneth W, Olsen, Catherine Putonti, Stefan M. Kanzok

Bioinformatics Faculty Publications

We recently identified three novel thioredoxin-like genes in the genome of the protozoan parasite Plasmodium that belong to the Phosducin-like family of proteins (PhLP). PhLPs are small cytosolic proteins hypothesized to function in G-protein signaling and protein folding. Although PhLPs are highly conserved in eukaryotes from yeast to mammals, only a few representatives have been experimentally characterized to date. In addition, while PhLPs contain a thioredoxin domain, they lack a CXXC motif, a strong indicator for redox activity, and it is unclear whether members of the PhLP family are enzymatically active. Here, we describe PbPhLP-3 as the first phosducin-like protein …

Research News. Publications, 2018. Volume 2, University Of Mississippi. School Of Pharmacy, Soumyajit Majumdar

Publications (2015-)

Publications that appeared during the period July 1 through December 31, 2018

Comparison Of Absolute Neutrophil Count Between Hospital And Community Acquired Methicillin-Resistant Staphylococcus Aureus Infection, Kurniyanto Kurniyanto, Widayat Djoko Santoso, Leonard Nainggolan, Juferdy Kurniawan

Jurnal Penyakit Dalam Indonesia

Introduction. The virulence factors from community acquired-methicillin resistant Staphylococcus aureus (CA-MRSA) mainly due to toxins like Panton Valentin Leukocidin (PVL) and Phenol Soluble Modulin (PSM). Both of toxins cause decrease of value through neutrophil lysis. This study aimed to identify different value of absolute neutrophil count between hospital and community acquired MRSA. Methods. A cross sectional was conducted which included subjects who were infected by MRSA and hospitalized during 2012-2017. Classification of MRSA were divided due to its sensitivity and resistance to non-beta lactam antibiotics. Isolate that resistance to ≤ 2 antibiotics were classified as CA-MRSA. The others with resistance …

Characterization Of A Protozoan Phosducin-Like Protein-3 (Phlp-3) Reveals Conserved Redox Activity, Rachel L. Kooistra, Robin David, Ana C. Ruiz, Sean W. Powers, Kyle J. Haselton, Kaitlyn Kiernan, Andrew M. Blagborough, Ken W. Olsen, Catherine Putonti, Stefan M. Kanzok

Chemistry: Faculty Publications and Other Works

We recently identified three novel thioredoxin-like genes in the genome of the protozoan parasite Plasmodium that belong to the Phosducin-like family of proteins (PhLP). PhLPs are small cytosolic proteins hypothesized to function in G-protein signaling and protein folding. Although PhLPs are highly conserved in eukaryotes from yeast to mammals, only a few representatives have been experimentally characterized to date. In addition, while PhLPs contain a thioredoxin domain, they lack a CXXC motif, a strong indicator for redox activity, and it is unclear whether members of the PhLP family are enzymatically active. Here, we describe PbPhLP-3 as the first phosducin-like protein …

Health Care Service For Leprosy Patients In Sitanala Tangerang (A Proposed Approach Of Comprehensive Health Care For Leprosy Patient), Yunia Irawati, Yeni Dwi Lestari, Anna Puspitasari Bani, Sri Linuwih Menaldi, Luh Karunia Wahyuni, Dewinta Retno Kurniawardhani

ASEAN Journal of Community Engagement

Based on WHO data, Indonesia has the third largest leprosy burden in the world, afterIndia and Brazil. Although leprosy was declared to be eliminated in severalprovinces, there are still several leprosy settlements spread in Indonesia. Oneof these settlements is Neglasari village in Tangerang City, West Java, whereleprosy patients have been living since 1981. There is a scarcity ofinformation regarding the features of leprosy in Indonesia, especially aboutthe description of the patients’ posttreatment condition in terms of bothclinical sequelae and health services provided. A collaboration betweenmultiple departments of Ophthalmology, Dermatovenereology, and MedicalRehabilitation from the Cipto Mangunkusumo Hospital/Universitas Indonesia,Jakarta, was established in …

Does Enforcement Reduce Voluntary Tax Compliance?, Leandra Lederman

BYU Law Review

Governments generally use enforcement methods, such as audits and the imposition of penalties, to deter noncompliance with tax laws. Although this approach is consistent with most economic modeling of tax compliance, some scholars caution that enforcement may backfire, “crowding out” taxpayers’ intrinsic motivations to pay taxes to such an extent that they reduce their tax payments. This Article analyzes the existing evidence to determine if this occurs. In fact, field studies suggest that enforcement tools, such as audits, are very effective deterrents. A few recent studies have found that audits have a negative effect on the subsequent tax payments of …

Cumulative Exposure To Solar Ultraviolet A & B Increases Apoptosis Of Peripheral Blood Cutaneous Lymphocyte Antigen (Cla)+ T-Lymphocytes In Outdoor Workers, Afif Nurul Hidayati, Saut Sahat Pohan, Widodo J. Pudjirahardjo, Isaac Effendy

Journal of General - Procedural Dermatology and Venereology Indonesia

Background: Exposure to ultraviolet A & B (UVA-UVB) plays a role in the survival of human life, but it may cause negative effects, such as immunosuppression and skin cancer. The effect of solar UVA-UVB exposure on apoptosis (Bax/Bcl-2 ratio) of peripheral blood cutaneous lymphocyte antigen (CLA)+ T-lymphocyte; the immune competent cells in the skin, has not been investigated. Apoptosis of peripheral blood CLA+ Tlymphocyte affects its function; which serves as the skin's resistance and is involved in infectious diseases, skin inflammation, and malignancies. This study observed the effect of solar UVA-UVB to apoptosis (Bax/Bcl2 ratio) of peripheral blood CLA+ …

Full Issue

Michigan Journal of Public Health

No abstract provided.

Faktor-Faktor Yang Berhubungan Dengan Kesuksesan Kesembuhan Dari Pengobatan Regimen Pendek (Short Treatment Regiment) Pada Pasien Tuberkulosis Resistensi Obat Di Indonesia Tahun 2017, Rina Agustina, Rizka Maulida, Yovsyah Yovsyah

Jurnal Epidemiologi Kesehatan Indonesia

TB RO menyebabkan beban pengendalian penyakit TB menjadi bertambah. Adanya penurunan angka keberhasilan pengobatan dari tahun 2010 sebesar 67,9% menjadi 51,1% di tahun 2013 dan peningkatan kasus pasien putus berobat mendorong Indonesia menerapkan pengobatan jangka pendek untuk meningkatkan angka keberhasilan pengobatan TB RO dan menurunkan kasus pasien putus berobat. Penelitian ini bertujuan untuk melihat status kesuksesan kesembuhan dari pengobatan TB RO dan faktor-faktor yang berhubungan dengan status kesuksesan kesembuhan dari pengobatan regimen pendek di Indonesia tahun 2017 dengan menggunakan data pasien TB RO yang tercatat dalam e-TB manager dari pasien-pasien berusia e” 15 tahun yang telah ada status hasil pengobatan …

Evaluasi Ekonomi Penggunaan Antibiotika Profilaksis Cefotaxime Dan Ceftriaxone Pada Pasien Operasi Seksio Sesarea Di Rumah Sakit X, Echa Aisyah, Mardiati Nadjib

Jurnal Ekonomi Kesehatan Indonesia

A prophylactic antibiotic for patients with cesarean section surgery is highly recommended in the clinical guideline. The use of antibiotics is selected by using criteria safety and cost-effectiveness. This partial economic evaluation in the form of cost and outcome analysis was using alongside observational study method, prospective data were collected from April to May 2017 covering 60 patients with cesarean section surgery who received prophylaxis antibiotics, comparing cost-effectiveness between Cefotaxime and Ceftriaxone. The statistical test showed that there were no differences of characteristics in the 60 patients. The analysis based on the hospital perspective carried out in a public hospital …

Recurrent Pneumonia In Children At A Tertiary–Pediatric Hospital In Baghdad, Muhi Kadhem Al-Janabi, Nadia Aziz Nasir, Rafed Kareem Jaber, Abbas Oweid Oleiwe

Iraqi Postgraduate Medical Journal

ABSTRACT: BACKGROUND: There are many children suffering from Recurrent Pneumonia (RP). Children with RP may present a difficult diagnostic challenge. OBJECTIVE: This study was performed to evaluate the frequency levels and underlying causes of RP in Children Welfare Teaching Hospital (CWTH)/Medical City/Baghdad. PATIENTS AND METHODS: In this prospective hospital-based cross-sectional study, 107 children admitted with RP in CWTH in the period from March to July 2014. Children were included in this study if they had at least 2 times pneumonia per year or three ever in life proved by radiographic confirmation during hospital admission. Children were classified according to underlying …

Checkpoint Inhibitors: What Gastroenterologists Need To Know., Monjur Ahmed

Division of Gastroenterology and Hepatology Faculty Papers

Checkpoint inhibitors are increasingly being used in clinical practice. They can cause various gastrointestinal, hepatic and pancreatic side effects. As these side effects can be serious, appropriate management is essential. The different checkpoint inhibitors with their mechanisms of action and indications, as well as evaluation and management of gastrointestinal, hepatic and pancreatic side effects, are discussed in this article.

Ole Miss Hall Of Famer Credited With 'Altering The Face Of Medicine', Tina Hahn

University of Mississippi News

Renowned immunologist Dr. Max Cooper wins Japan Prize

Using Physical Contact Heterogeneity And Frequency To Characterize Dynamics Of Human Exposure To Nonhuman Primate Bodily Fluids In Central Africa, Victor Narat, Mamdou Kampo, Thibut Heyer, Stephanie Rupp, Philippe Ambata, Richard Njouom, Tamara Giles-Vernick

Publications and Research

Emerging infectious diseases of zoonotic origin constitute a recurrent threat to global health. Nonhuman primates (NHPs) occupy an important place in zoonotic spillovers (pathogenic transmissions from animals to humans), serving as reservoirs or amplifiers of multiple neglected tropical diseases, including viral hemorrhagic fevers and arboviruses, parasites and bacteria, as well as retroviruses (simian foamy virus, PTLV) that are pathogenic in human beings. Hunting and butchering studies in Africa characterize at-risk human social groups, but overlook critical factors of contact heterogeneity and frequency, NHP species differences, and meat processing practices. In southeastern Cameroon, a region with a history of zoonotic emergence …

Pharmacy-Based Travel Health Services In The United States, Keri Hurley-Kim, Jeffery Goad, Sheila Seed, Karl M. Hess

Pharmacy Faculty Articles and Research

The aim of this paper is to review pharmacy laws and regulations, pharmacist training, clinic considerations, and patient care outcomes regarding pharmacy-based travel health services in the United States. Pharmacists and pharmacies in the United States are highly visible and accessible to the public, and have long been regarded as a source for immunization services. As international travel continues to increase and grow in popularity in this country, there is a pressing need for expanded access to preventative health services, including routine and travel vaccinations, as well as medications for prophylaxis or self-treatment of conditions that may be acquired overseas. …

Hematopoietic Stem And Progenitor Cells Are A Distinct Hiv Reservoir That Contributes To Persistent Viremia In Suppressed Patients, Thomas D. Zaikos, Valeri H. Terry, Nadia T. Sebastian Kettinger, Jay Lubow, Mark M. Painter, Maria C. Virgilio, Andrew Neevel, Frances Taschuk, Adewunmi Onafuwa-Nuga, Lucy A. Mcnamara, James Riddell, Dale Bixby, Norman Markowitz, Kathleen L. Collins

Infectious Diseases Articles

Long-lived reservoirs of persistent HIV are a major barrier to a cure. CD4+ hematopoietic stem and progenitor cells (HSPCs) have the capacity for lifelong survival, self-renewal, and the generation of daughter cells. Recent evidence shows that they are also susceptible to HIV infection in vitro and in vivo. Whether HSPCs harbor infectious virus or contribute to plasma virus (PV) is unknown. Here, we provide strong evidence that clusters of identical proviruses from HSPCs and their likely progeny often match residual PV. A higher proportion of these sequences match residual PV than proviral genomes from bone marrow and peripheral blood mononuclear …

Consideration Of Civil Cases Of Child Adoption In The Legislation Of The Republic Of Uzbekistan: Theory And Practice, G.Sh. Atalykova

Review of law sciences

The article discusses the theoretical and practical problems of the courts applying the law on adoption cases, the participation of the prosecutor and the guardianship authority, the requirements and basis for the cancellation of adoption.

Improving Wound Care Using The Time Framework, Pavel Kulikov

Doctor of Nursing Practice (DNP) Projects

Abstract

Background and purpose:Although wound care guidelines are available for primary care providers, barriers to assessment and treatment remain. This paper examines current evidence, guidelines, and discusses the need for improved training, education, and a simplified approach to wound management in primary care. The goal of the project was to increase the provider’s comfort level in assessing and initiating wound care treatment in the clinical setting. Methods:An evidence-based wound treatment framework, identified as the TIME (tissue, infection, moisture, epithelial) framework, was selected for the project. The framework was tailored by subject matter experts to provide a distinctive approach …

Differential Expression Of Genes Related To Innate Immune Responses In Ex Vivo Spinal Cord And Cerebellar Slice Cultures Infected With West Nile Virus, Parminder J.S. Vig, Deyin Lu, Amber M. Paul, Ram Kuwar, Maria Lopez, Dobrivoje S. Stokic, A. Arturo Leis, Michael R. Garrett, Fengwei Bai

Faculty Publications

West Nile virus (WNV) infection results in a spectrum of neurological symptoms, ranging from a benign fever to severe WNV neuroinvasive disease with high mortality. Many who recover from WNV neuroinvasive infection present with long-term deficits, including weakness, fatigue, and cognitive problems. While neurons are a main target of WNV, other cell types, especially astrocytes, play an important role in promoting WNV-mediated central nervous system (CNS) damage. Conversely, it has been shown that cultured primary astrocytes secrete high levels of interferons (IFNs) immediately after WNV exposure to protect neighboring astrocytes, as well as neurons. However, how intrinsic responses to WNV …

Differential Expression Of Genes Related To Innate Immune Responses In Ex Vivo Spinal Cord And Cerebellar Slice Cultures Infected With West Nile Virus, Amber M. Paul, Parminder J.S. Vig, Deyin Lu, Ram Kuwar, Maria Lopez, Dobrivoje S. Stokic, A. Arturo Leis, Michael R. Garrett, Fengwei Bai

Publications

West Nile virus (WNV) infection results in a spectrum of neurological symptoms, ranging from a benign fever to severe WNV neuroinvasive disease with high mortality. Many who recover from WNV neuroinvasive infection present with long-term deficits, including weakness, fatigue, and cognitive problems. While neurons are a main target of WNV, other cell types, especially astrocytes, play an important role in promoting WNV-mediated central nervous system (CNS) damage. Conversely, it has been shown that cultured primary astrocytes secrete high levels of interferons (IFNs) immediately after WNV exposure to protect neighboring astrocytes, as well as neurons. However, how intrinsic responses to WNV …

Bovine Mammary Gland Biopsy Techniques, Veridiana L. Daley, Charles Dye, Sophie H. Bogers, R. Michael Akers, Francisco C. Rodriguez, John P. Cant, John Doelman, Peter Yoder, Karan Kumar, Dane Webster, Mark D. Hanigan

Animal and Food Sciences Faculty Publications

Bovine mammary gland biopsies allow researchers to collect tissue samples to study cell biology including gene expression, histological analysis, signaling pathways, and protein translation. This article describes two techniques for biopsy of the bovine mammary gland (MG). Three healthy Holstein dairy cows were the subjects. Before biopsies, cows were milked and subsequently restrained in a cattle chute. An analgesic (flunixin meglumine, 1.1 to 2.2 mg/kg of body weight) was administered via jugular intravenous [IV] injection 15-20 min prior to biopsy. For standing sedation, xylazine hydrochloride (0.01-0.05 mg/kg of body weight) was injected via the coccygeal vessels 5-10 min before the …

70 Years Of Human Rights In Global Health: Drawing On A Contentious Past To Secure A Hopeful Future, Lawrence O. Gostin, Benjamin Mason Meier, Rebekah Thomas, Veronica Magar, Tedros A. Ghebreyesus

Georgetown Law Faculty Publications and Other Works

The Universal Declaration of Human Rights, adopted on Dec 10, 1948, established a modern human rights foundation that has become a cornerstone of global health, central to public health policies, programmes, and practices. To commemorate the 70th anniversary of this seminal declaration, we trace the evolution of human rights in global health, linking the past, present, and future of health as a human right. This future remains uncertain. As contemporary challenges imperil continuing advancements, threatening both human rights protections and global health governance, the future will depend, as it has in the past, on sustained political engagement to realise human …

Community Pharmacists And Substance Use Disorders: Attitudes, Knowledge And Practices, Deepika Rao

Electronic Theses and Dissertations

Background: Substance use disorder (SUD) is a serious health concern affecting a large proportion of the US population. Community pharmacists are well-positioned to prevent, screen, and provide appropriate treatment for SUD. Stigma towards these patients and SUD results in reduced quality of care as well as poor treatment outcomes. It is imperative that pharmacists have the appropriate knowledge of and attitude towards prevention, harm reduction and treatment to develop therapeutic relationships and improve their delivery of pharmacy services

Objective: To assess knowledge, practices (with an emphasis upon screening), stigma and general attitudes of community pharmacists towards SUD and evaluate the …

Access To Care And The Use Of Healthcare Services By Women In Rural Cameroon: A Participatory Action Research Study, Grace Tadzong-Awasum

Electronic Theses and Dissertations

Women in rural Cameroon do not access health care as timeously and as often as they should. They fail to get screened for common diseases, with the consequence that such diseases are often diagnosed at a very advanced stage. This accounts for the high morbidity and mortality rates as well as low life expectancy recorded among women. The purpose of this study was to explore and understand the health access experiences of women in rural Cameroon. Methodology: Participatory action research was used guided by a Focused ethnography in phase one. Twenty-five women aged 21-77 years were recruited through local the …

Harnessing The Anopheles Microbiome To Conditionally Express Anti-Plasmodial Effectors During The Blood Meal, Jackie Shane

Electronic Theses and Dissertations

The control of vector-borne diseases such as malaria has been an extremely important research subject for hundreds of years. Because of the complex lifecycles of the pathogens that cause these diseases, finding a comprehensive treatment or preventative strategy has proven extremely difficult. Malaria alone is responsible for almost half a million deaths annually, most of them children under 5 years old. This disease is caused by parasitic protists in the genus Plasmodium that are transmitted to humans from Anopheles sp. mosquitoes. Most preventative strategies that are in use today revolve around controlling the vectors, including bed nets, insecticides, and larval …

Probiotic Use In Trauma Patients: A Pathway To Determining The Standard Of Care To Improve Outcomes, Heather Vitko

Electronic Theses and Dissertations

The gut microbiome consists of normally non-pathogenic bacteria, viruses, and fungi. These friendly microbes serve to maintain GI barrier function and integrity, play a role in host nutrient and drug metabolism, immunomodulation, and prevent pathogenic bacteria from colonizing or causing disease. The importance of a healthy microbiome for the overall health of the host is just recently being appreciated within the medical and science communities. Disruption of the microbiome places one at greater risk for illness and infection. Patients who suffer a traumatic injury are among those at highest risk for complications associated with microbial imbalance, or dysbiosis. In addition …

Effects Of Cultural And Language Barriers On The Outcome Of Autism Spectrum Disorder Amongst Children From The Somali Immigrant Population Of Minnesota, Amal Farah

Theses and Graduate Projects

Autism Spectrum Disorder (ASD) is a devastating developmental disorder with a decline in both neurological and behavioral functions. ASK can present with delays in language development, social skills, and restricts behavioral patterns. The prevalence of ASD has been on the rise int he United States with a rate of one in 69 children. Moreover, the prevalence of ASD has been drastically increasing in the Somali community of Minnesota affecting one in 32 children. Many research studies associate the rise of ASD in the Somali community with the elements of culture, tradition, and language barriers. This research paper will explore how …

On Classification In Human-Driven And Data-Driven Systems, Qunwei Li

Dissertations - ALL

Classification systems are ubiquitous, and the design of effective classification algorithms has been an even more active area of research since the emergence of machine learning techniques. Despite the significant efforts devoted to training and feature selection in classification systems, misclassifications do occur and their effects can be critical in various applications. The central goal of this thesis is to analyze classification problems in human-driven and data-driven systems, with potentially unreliable components and design effective strategies to ensure reliable and effective classification algorithms in such systems. The components/agents in the system can be machines and/or humans. The system components can …

The Cuban Health Paradigm: An Exploratory Analysis Through Lgbtq And Hiv/Aids Individual Perspectives, Sarah Dryfoos

Hatfield Graduate Journal of Public Affairs

The purpose of this research project was to develop an understanding of the social, systemic, interpersonal, structural, and political dynamics of the health care system in Cuba. This was done by selecting two populations of focus; LGBTQ and HIV/AIDS positive individuals. The health care system and social determinants of health are analyzed using these two populations as lenses. While there is a plethora of information about the formal Cuban health care system, there is a dearth of literature pertaining to the social determinants, especially as it relates to LGBTQ Cubans and their quality of life and health care experience. To …