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A Comparison Of Phytoplankton Nutrient Limitation Between The Marsh And Beach Environments Of Waties Island, Sc, Raymond Sgambati, George Boneillo

Honors Theses

The aim of this study was to investigate the differences in nutrient limitation within the beach and marsh environments at Waties Island, SC. Conducting the experiment on Waties Island allowed most physical factors to be held as constant, meaning the marsh and beach environments would serve as the only variables. Experiments were performed in the winter, spring, summer and fall of 2018 to determine temporal changes in limitation to phytoplankton and cyanobacteria growth. Nutrient additions included dissolved inorganic nitrogen (nitrate, nitrite, and ammonium), dissolved organic nitrogen (urea), and phosphate. To test for co-limitation, a nitrate & phosphate treatment was also …

Development Of Polymeric Carriers For The Treatment Of Acute Kidney Injury, Yi Chen

Theses & Dissertations

Acute kidney injury (AKI) is a major kidney disease associated with high mortality and morbidity. Long-term AKI may lead to chronic kidney disease and end-stage renal disease. Several clinical trials failed due to lack of efficacy and undesired side effects. Studies showed that macromolecular delivery systems would be a promising method to target kidney, however, little is known about how physicochemical properties affects the polymers deposition in ischemia-reperfusion (I/R) AKI.

Gene therapy has been well studied as a promising therapeutic agent for several diseases, including cancer and AKI. Although small interfering RNA (siRNA) has been commonly used to treat AKI …

An Evaluation Of Deferred Time-Out, Abigail Kennedy

Theses & Dissertations

Although substantial research has shown time-out to be an effective treatment for children’s problem behavior, time-out resistance (i.e., failure to go to time-out, escape from time-out, as well as negative vocalizations and aggression occurring between the time-out instruction and the completion of time-out) has the potential to decrease time-out’s suppressive effect, increase use of more intrusive and effortful administrative methods and escape contingencies, and negatively affect parental adherence. The primary purpose of this study was to evaluate the effect of deferred time-out (DTO) on time-out resistance in a clinic and home setting. In addition, this research provided a preliminary evaluation …

Modulating Hallmarks Of Cholangiocarcinoma, Cody Wehrkamp

Theses & Dissertations

How are cholangiocarcinoma cells different from non-malignant cholangiocytes?

All of the hallmarks of cancer apply- those reported in this dissertation include cell proliferation, migration, apoptosis, and evasion of growth suppression. My studies began with testing how apoptosis might be regulated through embelin, a small molecule reported to sensitize cells to apoptosis by blocking XIAP. My data however revealed that embelin reduced the proliferative capacity in cholangiocarcinoma cells, but did not increase cell death. Malignant cells exhibit dysregulation of microRNA processing and expression. Hence, my studies seeking ways that cholangiocarcinoma eludes apoptosis transitioned to the oncomiR miR-106b, which is overexpressed in …

Regulation Of Canonical And Non-Canonical Hippo Pathway Components In Mitosis And Cancer, Seth Stauffer

Theses & Dissertations

The Hippo pathway is conserved regulator of organ size through control of proliferation, apoptosis, and stem-cell self-renewal. In addition to this important function, many of the canonical signaling members have also been shown to be regulated during mitosis. Importantly, Hippo pathway components are frequently dysregulated in cancers and have attracted attention as possible targets for improved cancer therapeutics. Further exploration of Hippo-YAP (yes-associated protein) signaling has revealed new regulators and effectors outside the canonical signaling network and has revealed a larger non-canonical network of signaling proteins in which canonical Hippo pathway components crosstalk with important cellular homeostasis and apoptosis signaling …

Towards An In Vitro Model Of Testing Osteoblast Cellular Function In Contact With Various Surfaces, Raheleh Miralami

Theses & Dissertations

Past studies have shown that the success of total joint replacements depends on the biocompatibility of orthopaedic materials, which can be improved by modifying the implant surface. However, the exact roles of these modifications and their effective mechanisms are poorly understood. The objective of this study was to develop and evaluate a model system to investigate the impact of nano-structured surfaces, produced by the ion beam-assisted deposition (IBAD) technique, on biomarkers of osteointegration using an in vitro model. The IBAD technique was employed to deposit zirconium oxide (ZrO₂), Titanium oxide (TiO₂), and Titanium (Ti) nano-films on …

Sequence-Specific Extracellular Micrornas Activate Tlr7, Niming Wu

Theses & Dissertations

Toll-like receptors (TLRs) play an important role in the innate immune system. Emerging evidence shows that TLRs, especially endosomal TLRs, can participate in CNS diseases by increasing the production of proinflammatory cytokines via recognition of microRNAs (miRNAs), however which of the miRNAs are able to activate signaling and whether specific sequence motifs are involved remains incompletely defined. Here we found that numerous miRNAs induced TNF-a production across multiple myeloid cell types, including microglia, and that this effect was abolished in cells deficient of TLR7. In particular, miR-20a-5p and miR-148b-3p preferentially stimulate cytokine secretion compared to miR-20b-5p and miR-148a-3p, respectively, despite …

Functional And Mechanical Role Of Splice Variant Of Mucin4 (Muc4/X) And Trefoil Factors In Pancreatic Cancer Pathogenesis, Rahat Jahan

Theses & Dissertations

Pancreatic Cancer (PC) is one of the vicious cancers as it ranks third in the race of leading cause of cancer-related death. Lack of early diagnostic marker, poor understanding of molecular mechanism of the disease and failure to conventional chemotherapy makes this disease dreadful.

Mucin 4 (MUC4), a high molecular weight glycoprotein is one of the top differentially expressed molecules in PC while not expressed in normal pancreas. Accumulating evidence from our lab suggested its tumorigenic role in PC by increasing cell proliferation, invasion, chemotherapy resistance, tumor growth, and metastasis. Previously, our lab and other has identified 24 different splice …

Development Of Preclinical Magnetic Resonance Imaging Relational Database And Interactive Analytical Tool For Diffusion Tensor Imaging, Ahmad Tanwir

Theses & Dissertations

Databases have become an essential component for any organization to collect and manage the information. Similar to other domains, by implementing a database management system (DBMS), data storage, access, analysis and updates can be automated and optimized in the biological, medical and preclinical research areas. This work demonstrated the development of a preclinical database, which contains the processed results from different magnetic resonance imaging (MRI) techniques with relevant biological information. In addition, we designed and implemented an interactive analytical tool to format and analyze diffusion tensor imaging (DTI) data and automatically generate results in the forms of box plot, bar …

Rhetoric In Film: Three Explorations Of Influence In Documentaries And Digital Stories, Emily Knapp

Masters Theses, 2010-2019

This thesis is made up of three distinct articles, two written with the intention of publication while the third consists of a digital story and subsequent reflection on the process of creation. The first article serves to answer the question “Do documentary films inspire activism?” by analyzing data gained after surveying 266 members of the James Madison University community. The results suggest that viewers are moved to emotion when witnessing struggle but that they are moved to action when said action directly impacts their own life. The second article is a rhetorical analysis of the 2013 documentary film Blackfish. …

A Multiscale Study Of A Nickel Penetrator Striking A Copper Plate Under Very High Strain Rates, Yangqing Dou

Theses and Dissertations

The objective of this dissertation centers on gaining a better understanding of the structure - property - performance relations of nickel and copper through the advanced multiscale theoretical framework and integrated computational methods. The goal of this dissertation also includes to combine material science and computational mechanics to acquire a transformative understanding of how the different crystal orientations, size scales, and penetration velocities affect plastic deformation and damage behavior of metallic materials during high strain rate (> 103s-1) processes. A multiscale computational framework for understanding plasticity and shearing mechanisms of metallic materials during the high rate process was developed, which …

Archeota, Fall 2018, Jennifer Castle, Sarah Thornton, Courtney Hopkins, Anna Belle Rosen, Havilah Steinman, Erin Fussell, Julieta Garcia

Archeota

This is the Fall 2018 issue of Archeota, the official publication of the SJSU SAASC

Sustainably Managing Groundwater - Surface Water Interactions Within The Santa Rosa Plain Basin, Rhianna Frank

Master's Projects and Capstones

In this paper, I designed a strategy for implementation of sustainably managing groundwater-surface water interactions in the Santa Rosa Plain Basin in compliance with the requirements set forth by the Sustainable Groundwater Management Act of 2014. The research objectives for this analysis are: (1) to fully examine the requirements of the Sustainable Groundwater Management Act and the implications that implementation will have on the Santa Rosa Plain Basin, (2) to use technical data gathered regionally coupled with related policy design theory to analyze how groundwater and surface water interactions can be managed to meet the requirements set forth in this …

Development Of A Step Counting Algorithm Using The Ambulatory Tibia Load Analysis System For Tibia Fracture Patients, Arad Lajevardi-Khosh, Ben Tresco, Ami Stuart, Sarina Sinclair, Matt Ackerman, Erik Kubiak, Tomasz Petelenz, Robert Hitchcock

School of Medicine Faculty Research

Introduction: Ambulation can be used to monitor the healing of lower extremity fractures. However, the ambulatory behavior of tibia fracture patients remains unknown due to an inability to continuously quantify ambulation outside of the clinic. The goal of this study was to design and validate an algorithm to assess ambulation in tibia fracture patients using the ambulatory tibial load analysis system during recovery, outside of the clinic. Methods Data were collected from a cyclic tester, 14 healthy volunteers performing a 2-min walk test on the treadmill, and 10 tibia fracture patients who wore the ambulatory tibial load analysis system during …

Transcriptome-Wide Analysis Of Vsv Strains With Varying Ability To Block Nf-Κb, Thomas M. Russell

Theses

Vesicular stomatitis virus (VSV) is a negative sense, single-stranded RNA (ssRNA) virus that is currently studied for its uses as a vaccine viral vector and its potential in oncolytic therapy. The M protein of VSV is the active participant in the virus’s abrogation of the host innate immune response. This activity is thought to be due to the M protein’s ability to downregulate overall host transcription by blocking nuclear transport. A VSV mutant containing a D52G mutation in the M protein (22-20) has been shown to block nuclear transport while being defective as a suppressor of NF-κB activation. It is …

Employing Natural History Collections In The Aid Of Conservation: Streamlining An Approach To Model Species Distributions En Masse For The Preservation Of Biodiversity, Alice Fornari

Master's Projects and Capstones

Using species distribution models (SDMs) in Natural History Collections (NHCs) can influence how humans implement conservation changes in flora and fauna communities and ecosystems. Through the use of legacy data (old NHCs and their associated locality/collection information), data correction (background data or pseudo absences added to presence-only data), and the SDM software, Maxent (and its associated geographic information systems or GIS projected models), it has been shown that it is feasible to create a low budget protocol/setup to project the past, present and future of species population changes. This has been done in the past few decades as more collections …

Methanogens: Pushing The Boundaries Of Biology, Nicole R. Buan

Department of Biochemistry: Faculty Publications

Methanogens are anaerobic archaea that grow by producing methane gas. These microbes and their exotic metabolism have inspired decades of microbial physiology research that continues to push the boundary of what we know about how microbes conserve energy to grow. The study of methanogens has helped to elucidate the thermodynamic and bioenergetics basis of life, contributed our understanding of evolution and biodiversity, and has garnered an appreciation for the societal utility of studying trophic interactions between environmental microbes, as methanogens are important in microbial conversion of biogenic carbon into methane, a high-energy fuel. This review discusses the theoretical basis for …

Synthesis Of Doxorubicin-Based Prodrug And Activatable Mr Nanoprobe For The Imaging And Treatment Of Cancer, Bayan Dous

Electronic Theses & Dissertations

Magnetic Resonance Imaging (MRI) is increasingly being used as a diagnostic tool for cancer. We propose a novel molecular probe, Gadolinium-DTPA disulfide-bonded Doxorubicin (Gd-DTPA-SS-Doxo) encapsulated in iron oxide which could provide a dual modality for detecting malignant growth while simultaneously targeting treatment options. We first synthesized Gd-DTPA-SS-Doxo followed by encapsulation within the poly (acrylic acid) (PAA) coating of iron oxide nanoparticle (IONP), producing a nanoprobe (IO-Gd-DTPA-SS-Doxo) with quenched longitudinal spin lattice magnetic relaxation (T1). After receptor-mediated internalization, Gd-DTPA-SS-Doxo is released from the nanoprobe’s polymeric coating due to the acidic microenvironment of the tumor. When the molecular probe is subjected to …

Using Games To Teach English In Chinese High School Classroom, Wenyu Zhu

Master's Projects and Capstones

In the 20th century, English played an important role in international communication as an international language. English is a bridge between countries' economies, cultures, and trade. However, current English education in Chinese high schools is still test-oriented which is ineffective, and students are tired of it. Moreover, teachers also have trouble engaging students in the class. The purpose of this project is to create a curriculum for high school English teachers in China to use games to teach English language skills. Krashen’s (1982) Theory of Second Language Acquisition contains five main hypotheses which support this project. The project includes twenty-three …

Community, Preservation, And Street Art: A Proposal For San Francisco’S Mission District, Marissa Nadeau

Master's Projects and Capstones

The Latinx community is an integral part of San Francisco’s rich history. From Mexican missions in the late 1700s to an influx of immigrants from various Latin countries starting in the early 1900s, the Mission District (‘the Mission’) of San Francisco has served as a hub for this mix of residents, fondly called “Raza,” emphasizing the people of a community rather than the country they have come from. Wars and issues dealt in their homelands were close to the hearts of the entirety of the Latinx population of the Mission, and their voices and opinions were heard through a type …

Housing Condition Impact On Pae Learning And Bdnf Expression., Annette K. Fernandez Oropeza

Biomedical Engineering ETDs

Prenatal alcohol and early life stress exposure are associated with reduced levels of BDNF in the frontal cortex. Several studies have implicated (BDNF) in frontal cortex as important contributor of learning and memory. In the present study, we look to determine whether early housing conditions could mitigate the impact of prenatal alcohol exposure on learning as well as BDNF expression in sex-specific manner in frontal cortex of mice. Compared to control, prenatal alcohol mice expresses reduced total BDNF levels and displayed reduced freezing behavior response during contextual fear conditioning in both female and male mice. Female displayed increased BDNF levels …

Museum-School Partnerships: From Creative Discourse To Resource Development, Emma Doctors

Master's Projects and Capstones

This paper aims to explore museum-school partnerships and best educational practices within each entity's efforts to collaborate. The project surveys museum education and explores programmatic and curriculum development that help to serve as enrichment for students. These partnerships are important to the museum studies field as it fulfills a fiduciary responsibility that museums hold - to educate the public. By fulfilling the needs of both entities (museums and schools), creative discourse ensues, creating solutions for students to enrich their learning. I end with a project to propose the development of an exhibition resource guide that would combine the knowledge and …

Urbanely Chained: Issues Within A City, Esther Shittu

Capstones

Each city in the United States bears its own burdens. In New York, one glaring burden is transportation, the second is the criminal justice system that seems to be packaged into one main facility: Rikers Island. The video and animation below explore these two issues. In Urbanely Chained: 219 West, the problems are examined within the scope of a television news magazine show. In Urbanely Chained: Gladiator Island History, the deep-rooted history of Rikers is explored.

Every month, students in the Craig Newmark Graduate School of Journalism produce a news magazine show that highlights different human interest stories. As a …

The New World Of Pet Prosthetics, Anna Brooks

Capstones

When an abandoned dog was found in Brooklyn with his paw gnawed off, the animal shelter had two options: amputate the leg, or put the dog down. But neither seemed like a solution. So they decided to do something different, and fit the dog for a prosthetic leg. Prosthetics for people have been around for millennia, and now these devices are moving into the animal kingdom. This story explores the new world of animal prosthetics, and how these devices have changed the lives of animals like Mr. Stubbs — the first alligator with a prosthetic tail — and saved the …

Kinder Carnage, Talib Visram

Capstones

In the wake of school shootings, including Parkland, states and school districts have scrambled to protect against threats. But faced with no federal focus on gun control and little funding, schools have to make tough safety choices. Some have adopted novelty security gadgets; others have discussed arming teachers and even giving children tools to defend themselves. To some, this is the ideal solution; the answer to violence is more violence. And so, some schools are starting to resemble military camps. With reporting from a security conference in Florida, and a military camp for kids in Kentucky, this story takes a …

Genetics Of Sexually Dimorphic Development Of Butterfly Wing Patterns, Luis Fernando Rodriguez Caro

Theses and Dissertations

Butterfly wing color patterns result from the arrangement of monochromatic scales containing chemical pigments and a delicate architecture that can cause interference or diffraction of light, generating iridescent colors. The latter mechanism is known as structural coloration and, despite its ecological importance, little is known about the molecular mechanisms underlying the development of this trait. The Southern Dogface butterfly, Zerene cesonia, exhibits sexually dimorphic development of ultraviolet wing reflectance. Males possess a UV-reflective patch on the forewing that results from nano-structures on the wing scales, which are absent in females. This dimorphism offers an excellent opportunity to explore the genetic …

Quantifying Pre-Industrial To Mid-Late 20th Century Anthropogenic Lead, Mercury And Cadmium Pollution In Caribbean Marine Environments Using Skeletonized Sea Turtle Remains, Felicia L. Pena

Theses and Dissertations

Various lines of evidence indicate that levels of anthropogenic pollutants, such as lead, mercury and cadmium, have increased in terrestrial and atmospheric environments since the early 19th century and the advent of industrialization. While the exposure to these three trace elements is a global concern, this study focused primarily on marine environments located throughout the Caribbean. Using ICP-MS, this study aimed to detect and quantify anthropogenic pollutants, specifically lead (Pb), mercury (Hg) and cadmium (Cd), using skeletonized remains of sea turtles as biological proxies for environmental quality. Archaeologically derived (n=5) and mid-late 20th century (n=6) Hawksbill and Green turtles were …

Tailoring Math Curricula For Community Colleges: A Process Approach, Ira Lindsay Mcfarland Kinard

Theses and Dissertations

Community college instructors continue to search for effective instructional methods that promote student success in developmental mathematics courses such as intermediate algebra. According to research, mathematics is a major obstacle for students succeeding, persisting, and completing community college programs. Each student enrolled in intermediate algebra has an ACT sub-score of 16 to 18 or an ACCUPLACER sub-score of 57 to 75. Most community colleges in Mississippi use placement testing to determine the strengths and needs of students. However, students approach math with high anxiety, more than any other areas, which interferes with their learning. Research indicates that to improve students’ …

Dietary Polyunsaturated Fatty Acids Regulate Mammary Tumor Growth And Metastasis By Modulating Tissue Microenvironments, Saraswoti Khadge

Theses & Dissertations

Omega (ω)-6 and ω-3 polyunsaturated fatty acids (PUFAs) are essential fatty acids (FAs) and the precursors of pro- and anti-inflammatory mediators respectively. Epidemiological studies have shown a lower incidence of breast cancer (BC) in the countries where long-chain (LC)-ω-3FAs consumption is higher; however, the role of ω-3FAs in BC growth and metastasis is poorly understood. We used isocaloric, isolipidic ω-6 and ω-3 (contains LC-ω-3FAs) diets and a pair-fed model to evaluate the effects of dietary PUFAs in mammary tumor metastasis. Our studies have resulted in several novel observations including that dietary LC-ω-3FAs modulate mammary gland (MG) microenvironments in non-tumor bearing …

An Assessment Of Preparations Made In The United States For Highly Hazardous Communicable Diseases Following The 2014-2016 Ebola Virus Disease Epidemic, Jocelyn J. Herstein

Theses & Dissertations

The 2014-2016 Ebola virus disease (EVD) epidemic in West Africa was unprecedented in magnitude and scope. The threat of imported cases of EVD in the United States prompted the Centers for Disease Control and Prevention (CDC) to establish a tiered network of hospitals to enhance domestic isolation capacity, including the designation of select hospitals as Ebola treatment centers (ETCs). As of spring 2015, no information existed on the capacity, physical infrastructure, staffing models, or infection control protocols of these newly-established ETCs, nor was there information on other highly hazardous communicable diseases (HHCDs) these units would admit. Moreover, no documentation was …