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Systems Biology Approach For The Establishment Of Veterinary Bone Tissue Engineering: The Proteomics Of Cbm-Mscs And Cdpscs Osteogenic Models, Sirirat Nantavisai

Chulalongkorn University Theses and Dissertations (Chula ETD)

The utilization of canine mesenchymal stem cells (cMSCs) with multipotent capabilities has been regarded for possible therapy of incorrigible bone disease. Although various sources of cMSCs show similar characteristics, they are different in osteogenic potential due to their original cellular sources. This study was designed to globally explore and analyze the in vitro differentiation potential and behavior of canine bone-marrow derived mesenchymal stem cells (cBM-MSCs) and canine dental pulp stem cells (cDPSCs) toward osteogenic lineage. An in vitro osteogenic differentiation potential of the cells was preliminarily compared in terms of alkaline phosphatase activity assay and Von Kossa staining. Global study …

Population Pharmacokinetics And Pharmacodynamics Of Piperacillin In Critically Ill Patients During The Early Phaseof Sepsis, Waroonrat Sukarnjanaset

Chulalongkorn University Theses and Dissertations (Chula ETD)

Background: Piperacillin/Tazobactam is frequently used for empirical treatment in patients with sepsis. Pathophysiological changes during the early phase of sepsis have significant effects on pharmacokinetic/pharmacodynamic (PK/PD) behaviors. This study aimed to characterize the population PKs of piperacillin and investigate probability of target attainment (PTA) and cumulative fraction of response (CFR) of various dosage regimens in critically ill patients during the early phase of sepsis. Methods: Forty-eight patients treated with piperacillin/tazobactam were recruited. Five blood samples were drawn before and during 0-0.5, 0.5-2, 2-4 and 4-6 or 8 hours after administration. Free piperacillin concentrations were determined using HPLC. Population PKs was …

Color Stability, Water Sorption And Water Solubility Of Various Artificial Acrylic Resin Teeth, Piyaporn Foungfu

Chulalongkorn University Theses and Dissertations (Chula ETD)

Color stability, water sorption and water solubility of the acrylic resin artificial teeth are the success keys to the appearance and mechanical properties of the denture teeth. The objective of the present study was to compare the color stability, water sorption and water solubility of 3 commercial artificial teeth: linear PMMA (NEW), resin composite (CPX) and cross-linked PMMA (ANT) and 1 color-liked heat-polymerized acrylic resin (MAJ). 80 colume-shaped specimens (9 mm/ 2 mm thickness) were fabricated for color stability test using a spectrophotometer after immersing in water or coffee solution at 1, 7, 28, 56 and 84 days. 40 colume-shape …

Molecular Modeling On Potent Compounds Toward Envelope Proteins Of Dengue And Zika Viruses, Kowit Hengphasatporn

Chulalongkorn University Theses and Dissertations (Chula ETD)

More than half of the world's population has a high risk of dengue and Zika viral infection that is one of a leading cause of death and brain's abnormality in children whereas there are no effective prevention or therapeutic agents to cure this disease. Recently, the flavanone derivative (FN5Y) and epigallocatechin gallate (EGCG) have been reported the inhibiting efficacy against dengue and Zika viruses, however, the mechanism of inhibitors is still ambiguous. Nowadays, the computer-aided drug design (CADD) and structure-based virtual screening method (SBVS) become the important techniques to reveal insight into the inhibitor's binding pattern and help drug discovery …

The Future Of Independent Contractors And Their Status As Non-Employees: Moving On From A Common Law Standard, John A. Pearce Ii, Jonathan P. Silva

UC Law Business Journal

No abstract provided.

Front Matter, University Of Michigan Journal Of Law Reform

University of Michigan Journal of Law Reform

Front matter for University of Michigan Journal of Law Reform Vol. 51, Issue 3

The Effects Of Aicar And Rapamycin On Mitochondrial Function In Immortalized Mitochondrial Dna Mutator Murine Embryonic Fibroblasts, Vedad Delic, Kenyaria Noble, Sandra Zivkovic, Tam Anh Phan, Christian Reynes, Yumeng Zhang, Oluwakemi Phillips, Charles Claybaker, Yen Ta, Vinh B. Dinh, Josean Cruz, Tomas A. Prolla, Patrick C. Bradshaw

ETSU Faculty Works

Mitochondrial DNA mutations accumulate with age and may play a role in stem cell aging as suggested by the premature aging phenotype of mitochondrial DNA polymerase gamma (POLG) exonuclease-deficient mice. Therefore, E1A immortalized murine embryonic fibroblasts (MEFs) from POLG exonuclease-deficient and wild-type (WT) mice were constructed. Surprisingly, when some E1A immortalized MEF lines were cultured in pyruvate-containing media they slowly became addicted to the pyruvate. The POLG exonuclease-deficient MEFs were more sensitive to several mitochondrial inhibitors and showed increased reactive oxygen species (ROS) production under standard conditions. When cultured in pyruvate-containing media, POLG exonuclease-deficient MEFs showed decreased oxygen consumption compared …

Lab-Grown Meat: Food Savior Of The Future Or An Elusive Ambition?, Jayla Johnson

The Synapse: Intercollegiate science magazine

No abstract provided.

Front Matter

The Synapse: Intercollegiate science magazine

No abstract provided.

Building Blocks: How Fundamental Particles Interact With Us, Patrick Banner

The Synapse: Intercollegiate science magazine

No abstract provided.

Talking To Climate Deniers: A Road Map To Changing Minds, Anah Soble

The Synapse: Intercollegiate science magazine

No abstract provided.

You Can't Always Trust Your Gut, Kallie Jiang

The Synapse: Intercollegiate science magazine

No abstract provided.

Man’S Best Friend: The Evolution Of Technology Is A Double-Edged Sword, Tyler Duffrin

The Synapse: Intercollegiate science magazine

No abstract provided.

Defusing The Bomb: How Mammoths, A Russian Scientist, And His Tank Are Trying To Save The World, Roman Broszkowski

The Synapse: Intercollegiate science magazine

No abstract provided.

Front Matter

The Synapse: Intercollegiate science magazine

No abstract provided.

Gene Editing: Combating Malaria With Crispr/Cas9, Christine Craddock

The Synapse: Intercollegiate science magazine

No abstract provided.

International Collaboration In Cuban Rivers: When Oberlin Connects You To Scientists Across The World, Monica Dix

The Synapse: Intercollegiate science magazine

No abstract provided.

Interactions Between Two Key Amphibian Defenses To Batrachochytrium Dendrobatidis In Panamanian Glass Frogs (Espadarana Prosoblepon), Andi Levorse

Honors Theses

Research on the amphibian pathogen Batrachochytrium dendrobatidis (Bd), the causative agent of the lethal disease chytridiomycosis, has advanced from assessments of pathogenicity and species susceptibility to more specialized questions concerning the complex interactions between the pathogen, species-specific immune responses, and the environment. Our work examines the potential for interactions between the two most important innate immune defenses of frogs against Bd: secretions of antimicrobial peptides and communities of commensal cutaneous bacteria. While both defenses have been studied individually, little data are available to examine interactions between these defenses. We conducted our study with field captured Panamanian glass frogs …

Bartonella And Y. Pestis Screening In Ctenophyllus Armatus Terribilis For Conservation Application For The America Pika (Ochotona Princeps) In The Rocky Mountain Front Range, Hilary Rinsland

Honors Theses

As temperatures continue to disproportionally increase in alpine regions due to climate change, parasite spillover from increased pika-rodent contact could bring new diseases to a susceptible alpine specialist, the American pika (Ochotona princeps). Fleas collected from pika at four alpine and subalpine study sites in Boulder County, Colorado from 2011-2017 were screened for Y. pestis and Bartonella using conventional PCR methods. This is the first study to detect Bartonella in the American Pika flea Ctenophyllus armatus terribilis and to propose the presence of B. grahmii in the alpine region of the Rocky Mountains.

Environmental Monitoring Report January 2016-June 2017, Tennessee. Department Of Environment And Conservation.

Environmental Monitoring Report

No abstract provided.

A Prescription For Teaching The Law Of Reasonable Religious And Disability Accommodation, Kerri Lynn Stone

Marquette Benefits and Social Welfare Law Review

No abstract provided.

Comparative Toxicology Of Nio And Ni(Oh)₂ Nanoparticles, Melissa Hope Cambre

Masters Theses

“Understanding the potential toxicity of nanoparticles (NPs) is important to ensure that these new products do not impose harmful effects to human and environmental health. Paper I is a literature review in which we discuss characteristics of nanomaterials, with an emphasis on transition metal oxide nanoparticles that influence cytotoxicity. Identification of those properties may lead to the design of more efficient and safer nanosized products for various industrial purposes and provide guidance for assessment of human and environmental health risk. We then investigate biochemical and molecular mechanisms of cytotoxicity that include oxidative stress-induced cellular events and alteration of the pathways …

Reliquaires Pervertis. Inscriptions Du Deuil Chez Maupassant, Mirbeau Et Rodenbach, Elizabeth Emery

Department of World Languages and Cultures Faculty Scholarship and Creative Works

Dans cette étude il est question de la fascination pour les tombes, reliquaires et épitaphes à travers leur représentation dans des textes naturalistes et décadents de la fin du XIXe siècle. Alors qu’on commande de plus en plus de monuments funéraires, surtout dans les années 1880 et 1890, les écrivains tels que Maupassant, Mirbeau et Rodenbach se moquent de leurs inscriptions ; ils privilégient une vision moins historique (celle de l’Histoire, avec un grand H) et plus intime (celle de l’histoire, avec un petit h) de l’épitaphe, la dotant ainsi d’une fonction de moins en moins matérielle et de plus …

Environmental Remediation And Its Discontents: The Contested Cleanup Of Vieques, Puerto Rico, Katherine Mccaffrey

Department of Anthropology Faculty Scholarship and Creative Works

Since protest forced the US Navy off Vieques Island, Puerto Rico in 2003, the US military has embarked on one of the largest environmental remediation projects it has ever undertaken. This article explores the way a narrowly conceived, technocratic cleanup process is translated onto an island with a deep history of grassroots mobilization and antagonism towards federal authority. The Restoration Advisory Board (RAB) is a crucible for considering the uneasy dynamics of cleanup. US law enshrines the RAB as the principal venue for "public participation" in the cleanup process. However, the prevailing technocratic framework, constrained by under-resourced bureaucratic agencies, clashes …

An Eye-Tracking Study Examining The Role Of Question-Answer Congruency In Children’S Comprehension Of Only: A Preliminary Report, Lauren Covey, Caitlin E. Coughlin, Utako Minai

Department of Linguistics Faculty Scholarship and Creative Works

‘Crain’s puzzle’ is a term that has been used to describe children’s difficulty comprehending the focus operator only when it is in subject position (subject-only), showing a tendency to interpret only as if it preceded the verb phrase instead. While some researchers attribute children’s difficulty to impoverished pragmatics in the discourse (Hackl et al., 2015), others argue that children’s grammar fundamentally differs from adults’ Notley et al. (2009), yielding a debate regarding whether children’s misinterpretation reflects a non-adult-like linguistic representation of only or some computational burden on their processing of meaning. This study addresses this debate by using eye-tracking to …

Marketing Strategies During The Product Life Cycle In The Pharmaceutical Industry, Natasa Naneva

Walden Dissertations and Doctoral Studies

Development and implementation of effective marketing strategies during various stages of product life cycle in the pharmaceutical industry are critical to an organization's successful performance in the marketplace in the 21st century. Guided by the general systems theory developed by Bertalanffy and the evolutionary systems theory developed by Laszlo and Laszlo, the purpose of this single case study was to explore best practices among marketing managers within pharmaceutical companies related to marketing strategies during various stages of product life cycle. Data were gathered via semistructured interviews with 3 purposefully selected managers who have successfully developed marketing strategies in a central …

Women’S Work? The Relationship Between Farmwork And Gender Self-Perception, Jolene Smyth, Alexis Swendener, Emily Kazyak

Department of Sociology: Faculty Publications

Women have long been involved in agricultural production, yet farming and ranching have been associated with masculinity and men. In recent years women have become more involved and more likely to take active and equal roles on farms and ranches and thus increasingly are doing tasks that have been associated with masculinity. Prior work indicates that women are perceived by others as more masculine when they do these tasks, but less work has focused on the association between women’s involvement in farming and women’s own perceptions of their gender (i.e., how masculine or feminine they feel). Using 2006 survey data …

Red Pepper Seed Water Extract Inhibits Preadipocyte Differentiation And Induces Mature Adipocyte Apoptosis In 3t3-L1 Cells, Hwa-Jin Kim, Mi-Kyoung You, Young-Hyun Lee, Hyun-Jung Kim, Deepak Adhikari, Hyeon-A Kim

Department of Nutrition and Health Sciences: Faculty Publications

BACKGROUND/OBJECTIVES: Reducing the number of adipocytes by inducing apoptosis of mature adipocytes as well as suppressing differentiation of preadipocytes plays an important role in preventing obesity. This study examines the anti-adipogenic and pro-apoptotic effect of red pepper seed water extract (RPS) prepared at 4°C (RPS4) in 3T3-L1 cells.

MATERIALS/METHODS: Effect of RPS4 or its fractions on lipid accumulation was determined in 3T3-L1 cells using oil red O (ORO) staining. The expressions of AMP-activated protein kinase (AMPK) and adipogenic associated proteins [peroxisome proliferator-activated receptor-γ (PPAR-γ), CCAAT/enhancer-binding proteins α (C/EBP α), sterol regulatory element binding protein-1c (SREBP-1c), fatty acid synthase (FAS), and …

Intermolecular Rna Recombination Occurs At Different Frequencies In Alternate Forms Of Brome Mosaic Virus Rna Replication Compartments, Hernan Garcia-Ruiz, Arturo Diaz, Paul Ahlquist

Department of Plant Pathology: Faculty Publications

Positive-strand RNA viruses replicate their genomes in membrane-bound replication compartments. Brome mosaic virus (BMV) replicates in vesicular invaginations of the endoplasmic reticulum membrane. BMV has served as a productive model system to study processes like virus-host interactions, RNA replication and recombination. Here we present multiple lines of evidence showing that the structure of the viral RNA replication compartments plays a fundamental role and that recruitment of parental RNAs to a common replication compartment is a limiting step in intermolecular RNA recombination. We show that a previously defined requirement for an RNA recruitment element on both parental RNAs is not to …

Metagenomic Analysis Of Viruses Associated With Maize Lethal Necrosis In Kenya, Mwathi Jane Wamaitha, Deepti Nigam, Solomon Maina, Francesca Stomeo, Anne Wangai, Joyce Njoki Njuguna, Timothy A. Holton, Bramwel W. Wanjala, Mark Wamalwa, Tanui Lucas, Appolinaire Djikeng, Hernan Garcia Ruiz

Department of Plant Pathology: Faculty Publications

Background: Maize lethal necrosis is caused by a synergistic co-infection of Maize chlorotic mottle virus (MCMV) and a specific member of the Potyviridae, such as Sugarcane mosaic virus (SCMV), Wheat streak mosaic virus (WSMV) or Johnson grass mosaic virus (JGMV). Typical maize lethal necrosis symptoms include severe yellowing and leaf drying from the edges. In Kenya, we detected plants showing typical and atypical symptoms. Both groups of plants often tested negative for SCMV by ELISA. Methods: We used next-generation sequencing to identify viruses associated to maize lethal necrosis in Kenya through a metagenomics analysis. Symptomatic and asymptomatic leaf samples …