Digital Commons Network^™

Ecological And Indigenous Community Impacts Of Oil Spill Mortality In Alaskan Marine Ecosystems, Paul M. Suprenand, Carie Hoover, Cameron H. Ainsworth, L. Dornberger, C. J. Johnson

Marine Science Faculty Publications

While hydrocarbon exploration and extraction in the Arctic ebbs and flows, reduced sea ice has opened new travel routes across the Arctic. The opening of the Northwest Passage has allowed larger ships (including oil tankers) and higher traffic into remote regions. More ice loss is expected in the future. With this comes the potential for hydrocarbon spills. To quantify the ecosystem impacts of a spill in the Alaska North Slope region, an Ecospace model using the Ecopath with Ecosim software was developed. We highlight the impacts of four potential hydrocarbon contamination scenarios: a subsurface crude oil pipeline release, a surface …

การศึกษาการแสดงออกของโปรตีนที่ควบคุมการส่งสัญญาณที่เกี่ยวข้องกับระบบภูมิต้านทานของเซลล์เม็ดเลือดขาวในกระแสเลือดของผู้ป่วยมะเร็งปอดชนิดเซลล์ไม่เล็ก, จิรวดี สถิตย์เรืองศักดิ์

Chulalongkorn University Theses and Dissertations (Chula ETD)

ความเป็นมา เซลล์เม็ดเลือดขาวชนิดนิวเคลียสเดี่ยวในเลือดมีการเปลี่ยนแปลงที่สัมพันธ์กับมะเร็งในร่างกาย บ่งบอกถึงการพยากรณ์โรคและผลการรักษา และอาจใช้เป็นตัวบ่งชี้ทางชีวภาพในการตรวจคัดกรองมะเร็งปอดชนิดเซลล์ไม่เล็กได้ การศึกษานี้จัดทำเพื่อศึกษาการแสดงออกของโปรตีนที่ควบคุมการส่งสัญญาณที่เกี่ยวข้องกับระบบภูมิต้านทานของเซลล์เม็ดเลือดขาวชนิดนิวเคลียสเดี่ยวในเลือดของผู้ป่วยมะเร็งปอดชนิดเซลล์ไม่เล็กเปรียบเทียบกับอาสาสมัครกลุ่มควบคุม วิธีการศึกษา ขั้นตอนที่ 1 คัดเลือกโปรตีนที่สนใจโดยใช้ข้อมูลการแสดงออกของอาร์เอ็นเอของเม็ดเลือดขาวชนิดนิวเคลียสเดี่ยวของผู้ป่วยมะเร็งปอดชนิดเซลล์ไม่เล็กจากฐานข้อมูลอิเล็กทรอนิกส์ ขั้นตอนที่ 2 ศึกษาการแสดงออกของโปรตีนที่สนใจด้วยการย้อมอิมมูโนฟลูออเรสเซนต์ ขั้นตอนที่ 3 เปรียบเทียบการแสดงของออกโปรตีนบนเม็ดเลือดขาวชนิดนิวเคลียสเดี่ยวด้วยเครื่องตรวจและวิเคราะห์เซลล์อัตโนมัติจากเลือดของผู้ป่วยมะเร็งปอดชนิดเซลล์ไม่เล็ก 30 คนและอาสาสมัครกลุ่มควบคุม 30 คน ผลการวิจัย จากการย้อมการแสดงออกของโปรตีน 5 ชนิด ได้แก่ CLEC4A, CLEC4D, C5AR1, NLRP3 และ S100A12 พบว่าเม็ดเลือดขาวชนิดนิวเคลียสเดี่ยวจากเลือดของผู้ป่วยมะเร็งปอดชนิดเซลล์ไม่เล็กมีการแสดงออกของโปรตีน CLEC4A, CLEC4D และ NLRP3 เพิ่มขึ้น ในขณะที่การแสดงออกของโปรตีน C5AR1 และ S100A12 ลดลง เมื่อเปรียบเทียบกับเลือดจากอาสาสมัครกลุ่มควบคุม สรุปผลการวิจัย การแสดงออกของโปรตีนที่ควบคุมการส่งสัญญาณที่เกี่ยวข้องกับระบบภูมิต้านทานของเซลล์เม็ดเลือดขาวในเลือดของผู้ป่วยมะเร็งปอดชนิดเซลล์ไม่เล็กมีความแตกต่างจากกลุ่มควบคุมอย่างมีนัยสำคัญ และอาจจะเป็นตัวบ่งชี้ทางชีวภาพในการตรวจคัดกรองผู้ป่วยมะเร็งปอดในอนาคตได้

Rna-Seq Of Kaposi's Sarcoma Reveals Alterations In Glucose And Lipid Metabolism, For Yue Tso, Andrew V. Kossenkov, Salum J. Lidenge, Owen Ngalamika, John R. Ngowi, Julius Mwaiselage, Jayamanna Wickramasinghe, Eun Hee Kwon, John T. West, Paul M. Lieberman, Charles Wood

School of Biological Sciences: Faculty Publications

Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiologic agent of Kaposi's sarcoma (KS). It is endemic in a number of sub-Saharan African countries with infection rate of >50%. The high prevalence of HIV-1 coupled with late presentation of advanced cancer staging make KS the leading cancer in the region with poor prognosis and high mortality. Disease markers and cellular functions associated with KS tumorigenesis remain ill-defined. Several studies have attempted to investigate changes of the gene profile with in vitro infection of monoculture models, which are not likely to reflect the cellular complexity of the in vivo lesion environment. Our approach …

Molecular Mechanisms Of Protection From Hepatitis C Infection, Paraskevi Mandalou

Peninsula Medical School Theses

Hepatitis C virus (HCV) infection is a major global health burden affecting 1-2% of the world’s population. The majority of infected individuals will develop chronic infection and are at risk of cirrhosis and hepatocellular carcinoma. There is currently no preventative vaccine available for HCV. In the developed world, the highest HCV incidence and prevalence rate is amongst intravenous drug users (IDU). The duration, frequency of IDU, and sharing of drug injecting equipment contribute to particularly high rates of HCV infection in this population. Individuals at high risk of recurrent exposure to HCV infection from long term IDU have been recruited …

‘The Show Must Go On’: Amateur Musical Theatre As A Community Of Practice, Kevin Walsh

Theses

Amateur musical theatre has its roots in long-standing theatre traditions that include both professional and amateur production. Apart from the performance, which is the over-arching goal, participation in amateur musical theatre yields many other personal and social benefits in areas of learning, community and identity, aligning with Wenger’s concept of a ‘Community of Practice’.

Using an analytical framework based on previous studies in this area, this research investigates how amateur musical theatre functions as a ‘Community of Practice’. The research was conducted using an ethnographic case study of two contrasting local amateur productions. Experiential data and details regarding participant interactions …

The Relationship Between Social Anxiety And Conforming Behavior In A Computerized Task, Jeremy Vargas

Murray State Theses and Dissertations

This study assessed the relationship between social anxiety symptoms and conforming behavior in both ambiguous and unambiguous computerized context. The majority of the participants for the sample consisted of Caucasian (78%) females (71%) who completed the task entirely (N = 94). Measures of the Social Phobia Inventory (SPIN) were used to assess social anxiety, while a computer mediated problem-solving task was used to assess conforming behavior. It was hypothesized that conforming behavior would be greater in the ambiguous context compared to the unambiguous context; and that social anxiety scores would be correlated with higher conforming behavior. While a Wilcoxon …

Evolution Of Western Library Catalogs: The Rising Expectations Of Users, Junli Diao

Publications and Research

This paper traces the historical development of library catalogs from primitive catalogs in ancient times to current next generational catalogs, which are summarized into three stages: the agricultural catalog stage, the industrial catalog stage and the information catalog stage. In particular, this paper focuses on the discussion of the rise of users’ expectations on library catalogs at different stages and gives emphasis to what impact they have created accordingly.

Scope Of Constitutional Review Of Parliamentary Enactments: Revisiting The Legitimacy Of The Arbitrariness Standard A Critical Comment On Raj Bala V. State Of Haryana, Shivam -

National Law School of India Review

The Supreme Court's dicta in Raj Bala v. State of Haryana' ('Raj Bala') has renewed the juristic and scholarly interest in the appropriate form of equality analysis applicable to statutes. Ever since the enunciation of a seemingly 'new' standard for determining the negation or otherwise of the right to equality in the celebrated case of E.P. Royappa v. State of TN. 2 ('Royappa'), jurists and academicians alike have struggled to put this development in perspective. Even as the controversy surrounding the doctrine was far from clear, a division Bench readily dismissed the applicability of 'arbitrariness' standard to invalidate principal legislations. …

Effects Of Large-Scale Density Reduction On Brown Trout Growth And Movement In Spearfish Creek, South Dakota, Travis Rehm

Electronic Theses and Dissertations

Density-dependent growth is often observed in stream-dwelling Brown Trout Salmo trutta populations. In Spearfish Creek, South Dakota, biomass of adult Brown Trout (>200 mm) is about three times greater than that reported for similar Black Hills streams while mean length of adult fish is about 30% less. Here, we evaluate large-scale density reduction as a management tool for improving growth rate of stream-dwelling Brown Trout. We compared age-specific growth of wild Brown Trout in stream sections receiving 50% reductions in fish abundance. We also assessed the effect of fish density manipulation on Brown Trout movement patterns and home range …

The Influence Of Spore-Forming Microorganisms On The Quality And Functionality Of Cultured Dairy Products, Dipakkumar Mehta

Electronic Theses and Dissertations

Spore formers are observed as the common spoilage-causing microflora in milk and dairy products. Their spore-forming ability increases their survivability during heat treatment which leads to their presence in a final product. Spore-forming bacteria have ability to produce many types of spoilage-causing enzymes which are potential to influence quality and functionality of the final product. Many researchers have studied the ability of spore formers to induce a spoilage in various dairy products but as per our knowledge, their spoilage-causing activities are not described in a detail for cultured dairy products. In our first objective, we have studied the ability of …

Understanding City Parks As New Common Pool Resources: A Case Study Of The Dakota Nature Park, Keahna Margeson

Electronic Theses and Dissertations

This thesis examines public parks as New Common Pool Resources through a case study of the Dakota Nature Park in Brookings, South Dakota. I identify the formalization and bureaucratization processes experienced by the governing body of the park. These processes occurred as a capped landfill was repurposed and collaboratively managed to serve the community by providing native, natural space and affordable recreational opportunities. The governing structure is assessed using Elinor Ostrom’s (1990) Eight Principles of Common Pool Resource Management, Weber’s (1964) ideas of status and authority and Berger and Luckman's (1966) phenomenological theory. I use three major research strategies: (1) …

Comparison Of Retaining Replacement Heifers In A Straight-Bred Angus System Versus Purchasing Crossbred Replacement Heifers To Be Bred To A Terminal Sire, Grady D. Ruble

Electronic Theses and Dissertations

Two evaluations were conducted to evaluate the biological and economic production efficiency of retaining replacement females in a straight-bred Angus herd (HR) as opposed to purchasing crossbred replacements for a terminal sire production system (PR) where maximum maternal and individual heterosis is achieved. The first study was a comparison of the HR system versus PR system. It was modeled after a 100 hd, straightbred Angus cowherd that raises their own replacement females. The (NASEM, 2016) model was used to determine the required metabolizable energy (ME)/animal for each stage of production. Stages were broken into segments including weaning – d 28, …

Diversity Of Free – Living Nitrogen – Fixing Bacteria In Soil Of Sioux Prairie Of South Dakota, Nabilah Ali Alshibli

Electronic Theses and Dissertations

There are only two natural ways by which molecular nitrogen can be available to support life, either by free-living or by symbiotic nitrogen fixing bacteria. The best studied diazotrophs form symbiotic associations with plants, primarily legumes and certain tree species, but little is known about how non-leguminous plants such as grasses obtain nitrogen in their environment. Natural prairies have few legumes, thus have less symbiotic nitrogen fixer interaction. This indicates presence of free-living nitrogen (FLN) fixation activity towards the balance of the N cycle. The objective of this study was to characterize the culturable diversity of free-living diazotrophs in native …

Can Solvent-Or Mechanically-Extracted Carinata Meal Be Used As An Effective Source Of Supplemental Protein To Cows Fed Poor Quality Forages?, Emily Jacklyn Rosenthal

Electronic Theses and Dissertations

Brassica carinata is an oilseed crop that requires a relatively short growing season and produces high crop yields. It is a great source for aviation biofuel, however it produces a large amount of waste that may be used as a protein supplement for cattle. We tested the effects of solvent- or mechanically-extracted carinata meal as a protein supplement to ad libitum ground or long-stem corn residue (i.e., corn plant left over from grain harvest) had on cow performance and digestibility. Fifty-six non-pregnant cows in 8 pens had ad libitum access to either long-stem or ground corn residue in addition to …

Strategies To Improve Calf Performance: Improving Water Quality, Feeding Microbially-Enhanced Soy Protein, And Supplementing Condensed Whey Solubles, Nirosh Dias Senevirathne

Electronic Theses and Dissertations

Six experiments were conducted for this dissertation research to evaluate strategies to improve growth performance and health of calves. Growth performance, nutrient utilization, and health effects were evaluated when calves were offered water treated with reverse osmosis system (RW), fed starter pellets supplemented with microbially-enhanced soy protein (MSP) or supplemented condensed whey solubles (CWS). The first two experiments were conducted to determine drinking preference, growth performance, and health of dairy calves offered RW or by a municipal water treatment plant (MW), compared with local untreated well water (WW). First a sequential elimination study was conducted to observed drinking preference of …

Gtpase-Activating Protein-Binding Protein 1 Plays An Antiviral Role In Porcine Epidemic Diarrhea Virus (Pedv) Replication, Kabita Pandey

Electronic Theses and Dissertations

Porcine Epidemic Diarrhea Virus (PEDV) is a non-segmented virus which uses one-stranded, positive-sense RNA as genetic material. PEDV falls under Coronaviridae family and extensive diarrhea and dehydration in nursing piglets are the major clinical signs. This emerging disease leads to huge economic loss in the pig farming. Very less studies are done towards the role of innate immunity in PEDV infection. The formation of Stress granules (SGs) are seen when cells are introduced to different stressful conditions, which also includes viral infections. SGs are formed when one of the four kinases: doublestranded RNA (dsRNA)-dependent protein kinase (PKR), PKR-like endoplasmic reticulum …

Synthesis, Characterization And Molecular Dynamic Simulations Of Aqueous Choline Chloride Deep Eutectic Solvents, Sampson Asare

Electronic Theses and Dissertations

Various forms of deep eutectic solvents have been synthesized with advantages of 100% atom economy, low vapor pressure, ease of preparation, cost efficiency, low toxicity, and tunability. Though several DESs have been synthesized and characterized, including binary and ternary solvents, none of the binary DESs have been formulated with water as the sole hydrogen bond donor(HBD). This is due to a couple of opinions. First, water is disruptive to the eutectic bond in tertiary DES. Secondly, the ubiquity and concentration of water even in natural systems have led to the assertion that a solution rather than a eutectic solvent will …

Exploiting The Genetic Diversity Of Wild Ancestors And Relatives Of Wheat For Its Improvement, Jagdeep Singh Sidhu

Electronic Theses and Dissertations

Wheat is the third most staple food worldwide but current 1% annual improvement in the wheat production is insufficient to meet the growing demands in future. The narrow genetic base of wheat limits continuous improvement in wheat productivity and tolerance to biotic and abiotic stresses under changing climate. Wild ancestors and relatives of wheat hold a potential in widening the genetic pool of wheat and enhance its resilience to biotic and abiotic stresses. This study was focused towards characterizing the genetic diversity in wild relatives of wheat for disease resistance and efficient association with diazotrophs. In the first study, we …

Development Of An Odor Management Plan For South Dakota, Suraiya Akter

Electronic Theses and Dissertations

Odor from livestock operations is often a nuisance to the neighborhood stakeholders and is one of the major environmental and societal issues associated with livestock industries. An odor management plan is not a requirement during the state permitting process of any livestock operation in South Dakota, but may be required to varying degrees for county level approval. Some neighboring states have developed odor management planning guides or templates which help address odor issues. Adoption of an odor management plan in SD can help address concerns of odor for the continuously expanding livestock industry in this state. Hence, an odor management …

Study Of Anti-Proliferative Activity Of Cucurbitacins Inspired Estrone Analogs On Hepatocellular Carcinoma, Sara Elgazwi

Electronic Theses and Dissertations

Hepatocellular carcinoma (HCC) is considered the third leading cause of death from cancer. Overall survival rate is significantly low, due to the emerging resistance to chemotherapeutic agents and lack of selectivity. Recent studies have demonstrated that epidermal growth factor receptor (EGFR) is a promising molecular target for cancer therapy, especially HCC. Current studies showed that cucurbitacins are potent anticancer compounds which target EGFR. This prompted us to investigate the antiproliferative activity of novel cucurbitacins inspired estrone analogs (CIEA) against sensitive and resistant HepG2 cell lines. Anti-proliferation activity of 20 CIEA analogs were examined against HepG2 using MTT assay and showed …

Evaluation Of Dietary Phytochemicals As Rumen Modifiers In Lactating Dairy Cows, Sonia Rodriguez Jimenez

Electronic Theses and Dissertations

Phytochemicals such as tannins included in dairy cow nutritional programs, as a natural feed additive, promise to improve rumen bypass protein and consequently reduce the feeding cost by decreasing the amount of CP needed in the ration. Additionally, if tannins are fed along with high quality protein sources, this could improve the AA profile reaching the small intestine and potentially improving health and performance of dairy cows. The black pepper, specifically its alkaloid piperine, is a nontoxic, natural dietary compound with a broad range of physiological activity. Lactating dairy cows ingesting these phytochemicals (tannin extracts and black pepper) will potentially …

Efficiency Of Utilizing Standardized Ileal Digestible Lys And Thr For Whole Body Protein Retention In Pregnant Gilts During Early, Mid And Late Gestation, Ron Aldwin Sapin Navales

Electronic Theses and Dissertations

In pregnant pigs, amino acid (AA) requirements represent the sum of those required for maintenance functions, protein retention and efficiency of utilizing AA intake for the aforementioned body processes. The NRC (2012) model assumed AA efficiency is constant across period of gestation; however this is not reflective of the changes in metabolic demand during gestation. Therefore, two experiments were conducted to evaluate the efficiency of utilizing SID Lys and Thr for whole body protein retention (kSIDLys and kSIDThr) in pregnant gilts during early, mid and late gestation. Three 12 d N-balance studies were conducted to represent different periods of gestation. …

Application Of Genomic Approaches To Improve Yield And Bacterial Leaf Streak Resistance In Winter Wheat, Sai Mukund Ramakrishnan

Electronic Theses and Dissertations

Global wheat production is threatened by the change in climate thus leading lead to the increase in the biotic and abiotic stresses. We need to increase wheat productivity at a faster pace and manage these challenges to meet the growing demand. Development of cultivars with durable disease resistance and enhancing the rate of genetic gain in wheat are the major goals in wheat breeding programs. Bacterial Leaf Streak (BLS) is one of the most threatening bacterial diseases to wheat in the US Northern Great Plains. Unlike fungal diseases, bacterial diseases cannot be effectively managed using chemicals and thus developing disease …

Optimization Of Microfluidic Particle Separator Geometry Using Computational Fluid Dynamics, Joseph Petersen

Electronic Theses and Dissertations

Computational fluid dynamics software was used to simulate the motion of circulating tumor cells in a variety of microfluidic cell isolation devices. Design of several novel microfluidic cell isolation devices was aided by viewing streamlines of fluid in devices in simulation. Devices that performed best in simulation used 5-micrometer wide guiding channels to guide cells to the capture location in the device. While these devices performed better than other devices in simulation and captured all particles regardless of position along inlet, experimental results differ from simulation.

Influence Of Production System On Animal Performance, Carcass Characteristics, Meat Quality, Environmental Impacts, Production Economics, And Consumer Preference For Beef, Megan Jean Webb

Electronic Theses and Dissertations

The overall objective of this study was to determine if the level of growth promotant technology used among production systems influence animal and carcass performance, meat quality, production economics, the environmental impact, and determine consumer preferences and perception. Angus � Simmental steer calves (n =120) were stratified by birth date, birth weight, and dam age in a completely randomized design and assigned to one of four treatments: 1) no antibiotics (NA, receiving no technology); 2) non-hormone treated (NHTC, fed monensin and tylosin); 3) implant (IMPL, administered a series of three implants), and 4) implant plus fed a beta-agonist (IMBA, administered …

Satellite Remote Sensing Of Woody And Herbaceous Leaf Area For Improved Understanding Of Forage Resources And Fire In Africa, Milkah Njoki Kahiu

Electronic Theses and Dissertations

In sub-Saharan Africa (SSA) tree-grass systems commonly referred to as savannas dominating drylands, play a critical role in social, cultural, economic and environmental systems. These coupled natural-human systems support millions of people through pastoralism, are important global biodiversity hotspots and play a critical role in global biogeochemical cycles. Despite the importance of SSA savannas, they have been marginalized for years as most governments neglect dryland resources in favor of agricultural research and development assistance. Hence, lack of spatially and temporally accurate information on the status and trends in savanna resources has led to poor planning and management. This scenario calls …

Universal Swine Influenza Antigen And Vaccine, Zhao Wang

Electronic Theses and Dissertations

Swine influenza A viruses pose a serious concern for global health and worldwide swine industry considering their evasion from vaccine-induced immune responses. Formulation of effective influenza vaccines can be complicated by both antigenic shift to a different subtype of hemagglutinin (HAs) and antigenic drift within a particular HA subtype. To address these concerns, we employed a systematic approach to determine the antigenic determinants of swine influenza A viruses by focusing on H1 and H3 subtypes. In parallel, we used a cross-disciplinary approach including structural biology and modeling, virology, and immunology to identify swine influenza A virus-derived HA fusion intermediates broadly …

Enhancing The Nutritive Value Of Corn Ddgs For Pigs, Casey Zangaro

Electronic Theses and Dissertations

Corn dried distillers’ grains with solubles (DDGS) can be a good alternative feedstuff to the traditional corn soybean meal diets since it has high fat and protein content. However, it has a high fiber content, which is not well digested by pigs and can reduce nutrient utilization by encapsulation. The nutritive value of fibrous feedstuffs like DDGS can be improved by supplementation with fiber-degrading enzymes. However, fiber-degrading enzymes have not been effective in improving digestibility of DDGS. The overall goal of this thesis research to was to unravel why pigs poorly digest DDGS and to develop strategies that can increase …

Serological Evidence For The Co-Circulation Of Two Lineages Of Influenza D Virus In Equine Populations Of The Midwest United States, Hunter Theodore Nedland

Electronic Theses and Dissertations

Influenza D virus (IDV) is a newly described lineage of the Orthomyxoviridae virus family that was first isolated from diseased swine in 2011 and has subsequently been detected in cattle around the world in 2014. In addition, serological evidence for IDV infection in humans has been recently established. Despite all the progress, the full range of susceptible hosts for this novel virus has yet to be determined, but includes swine, bovine, small ruminants and human. This study was designed to determine if equine is a possible host to this newly emerging influenza virus. 364 equine serum samples were collected in …

A Pcr/Sequencing Technique To Identify Species And Genotypes Of Haemonchus From Mixed Trichostrongyle Egg Dna Recovered From Livestock Fecal Samples, Pratiksha Khanal

Electronic Theses and Dissertations

Six primer pairs, each with a pair of genus-specific and universal second internal transcribed spacer (ITS2) primers were evaluated to amplify and sequence the ITS2 region of nuclear ribosomal DNA to identify the species and genotypes of Haemonchus from livestock fecal samples containing mixtures of trichostrongyle nematode eggs. Based upon the use of universal ITS2 primers on Haemonchus adults, the ITS2 region has been shown to contain three fixed single-nucleotide differences between H. placei and H. contortus. It is clinically useful to identify the species and genotypes of Haemonchus infecting livestock, but it has not been possible in fecal …