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2018

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The Effects Of Brefeldin A On Dictyostelium Discoideum In Single And Co-Culture Environments, Spencer Hills Jan 2018

The Effects Of Brefeldin A On Dictyostelium Discoideum In Single And Co-Culture Environments, Spencer Hills

Theses & ETDs

Cells of some species of slime mold, as well as the model amoeba Entamoeba invadens, are attracted to the furrowing region of dividing cells in telophase, physically assisting neighboring cells with cytokinesis in a process known as cytokinetic midwifery. However, little is known with respect to possible inter-species interactions or chemotactic behavior related to the cytokinetic midwifery phenomenon. In an attempt to stimulate cytokinetic midwifery, brefeldin A (BFA), a secretory pathway inhibitor and known inhibitor of cytokinesis in Caenorhabditis elegans, was applied to experimental agar plates upon which the slime mold Dictyostelium discoideum was cultured, and cells were monitored for …

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I Am Here: The Journey And Impact Of Makoto Shinkai’S Independently Developed Animated Short Film “Voices Of A Distant Star”, Christian Ines Jan 2018

I Am Here: The Journey And Impact Of Makoto Shinkai’S Independently Developed Animated Short Film “Voices Of A Distant Star”, Christian Ines

Theses & ETDs

The purpose of this thesis is to review the process that Makoto Shinkai takes in his production of the animated film Voices of a Distant Star, as well as relate it to my own personal journey as a creative artist. Using a DVD copy with official English subtitles I begin by reviewing the initial production of the film from the small beginnings of forming the idea to the large scale success that was brought from it. I also relate the story of the film as an extended metaphor towards Shinkai’s creative process as well as my own. In Chapter 2 …

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A Guide To Selected Herpetofauna Of Cayo Cochino Grande, Honduras, Kasia Wake Jan 2018

A Guide To Selected Herpetofauna Of Cayo Cochino Grande, Honduras, Kasia Wake

Theses & ETDs

A series of nine exploratory visual encounter surveys were conducted on Cayo Cochino Grande, Honduras from 7/7/17 to 7/17/17. The object of this study was to take inventory of the island’s herpetofauna composition with the intention to set up a species guide book and potentially establish a framework for future surveys. Three predetermined paths including beach, mangrove forest, hill forest, and residential habitats, were each surveyed in the morning, afternoon, and night. The following organisms were observed during surveying: Phyllodactylus palmeus, Hemidactylus frenatus, Anolis allisoni, Iguana iguana, Basiliscus vittatus, Ctenosaura melanosterna, Norops lemurinus, Oxybelis aeneus, and Boa constrictor imperator. Photographic …

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Acquired Characteristics Of Epigenetics, Cassandra Ratzlaff Jan 2018

Acquired Characteristics Of Epigenetics, Cassandra Ratzlaff

Theses & ETDs

The world of genetics is always changing. Today, current techniques in genetics allow for screening of genetic disorders, mapping of gene functions, and gene editing. Genetics explains heredity and variations of inherited characteristics. A species is said to have evolved if there is a change in the genetic composition that has been inherited through multiple generations. This does not however, explain if genes can be altered and passed on through a single inheritance. Epigenetics is an emerging field of study that describes how genes can be turned “off” and “on” by external factors in a single organism and do not …

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Magna Latrocinia: Money And Governance In England And Northern Italy Between The Tenth And Fifteenth Centuries, Victoria Deal Jan 2018

Magna Latrocinia: Money And Governance In England And Northern Italy Between The Tenth And Fifteenth Centuries, Victoria Deal

Theses & ETDs

This thesis examines money as a part of developing systems of government in the middle ages. In England, the recreation of counterfeiting as a crime and the enforcement of the nominal value of money as distinct from its value as bullion reflected the creation of a centralized English state over the tenth through thirteenth centuries. In northern Italy, independent city-states used money and regulation of the cash economy as part of an effort to establish distinct identities and legitimate their rule between the twelfth and fifteenth centuries. Both societies contended with the subsequent development of finance, and the pressure of …

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The Relationship Between The Feeding Habits And Behavior Of Cyphoma Gibbosum (Linnaeus, 1758) And The Spread Of Aspergillus Sydowii (Thom & Church, 1926) Affecting Gorgonia Ventalina (Linnaeus, 1758) At Cayos Cochinos, Honduras, Kristin Spoto Jan 2018

The Relationship Between The Feeding Habits And Behavior Of Cyphoma Gibbosum (Linnaeus, 1758) And The Spread Of Aspergillus Sydowii (Thom & Church, 1926) Affecting Gorgonia Ventalina (Linnaeus, 1758) At Cayos Cochinos, Honduras, Kristin Spoto

Theses & ETDs

Coral reefs are some of the Earth’s most highly diverse ecosystems, housing millions of organisms. The organisms most important to coral reefs second to the reef building coral are the soft corals, which provide a source of food, habitat, nutrient cycling as well as other benefits to the organisms living on the reefs. These soft corals, including gorgonians, are sensitive and experiencing an increasing rate of disease and mortality worldwide. One such disease is a pathogenic fungus called Aspergillus sydowii and has seen to be prominent amongst Gorgonia ventalina, the sea fan. Mechanisms of fungal infection are not certain at …

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Impact Of Urbanization On Salinity Of Sarasota Bay Tidal Creeks, Claudia Vila Jan 2018

Impact Of Urbanization On Salinity Of Sarasota Bay Tidal Creeks, Claudia Vila

Theses & ETDs

Urbanization of watersheds causes more runoff to be discharged into surrounding estuaries. Some studies have connected increased discharge to decreased salinity in the estuaries. Salinity is a very important characteristic of these ecosystems, because it can influence biotic distribution. The Sarasota Bay watershed is highly urbanized and stormwater runoff is an established consequence of development. In this study, I looked at the impact of urbanization on salinity in Sarasota Bay, Florida. To detect variation through space and through time, I used spatial data that indicates percent imperviousness, salinity data collected at the mouths of each tidal creek, and time series …

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A Clove A Day Keeps The Dentist Away? A Proposal To Investigate The Efficacy Of Natural Quorum Quenching Compounds On Streptococcus Mutans Biofilms, Alexis P. Pujol Jan 2018

A Clove A Day Keeps The Dentist Away? A Proposal To Investigate The Efficacy Of Natural Quorum Quenching Compounds On Streptococcus Mutans Biofilms, Alexis P. Pujol

Theses & ETDs

The formation of microbial biofilms on human teeth, also known as dental plaque, can lead to periodontal diseases and dental caries. The current treatments for these ailments are costly and inaccessible for many people, but if left untreated, these diseases can lead to serious illnesses. Bacteria within a biofilm communicate using quorum sensing (QS) and utilize this method to count themselves for controlled gene expression. In recent years, there has been significant research on how to inhibit this inter-bacterial communication in a process called, quorum quenching (QQ). Molecules that closely resemble the QS molecules produced and accepted by bacteria can …

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Neonicotinoids: A Comparative Pesticide Policy Analysis Between The European Union And The United States, Tara Olson Jan 2018

Neonicotinoids: A Comparative Pesticide Policy Analysis Between The European Union And The United States, Tara Olson

Theses & ETDs

Neonicotinoids are an insecticide group, introduced in the 1980s, as a more effective method to combat insect pests. Within the past decade, unexpected side-effects of neonicotinoids have been discovered. The most prominent species that has shown negative side effects is the European honey bee, Apis mellifera. By targeting the nervous system, chronic, sub-lethal exposure to neonicotinoids causes behavioral changes and neurological damage, leading to eventual mortality in this species. The United States and the European Union are both affected by the loss of this globally essential pollinator. However, they have taken diverging approaches to manage the risk to this pollinator …

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Wittgenstein's Coffee: An Exploration Of Food/Art, Conceptual Art, And Context, Miranda Gale Jan 2018

Wittgenstein's Coffee: An Exploration Of Food/Art, Conceptual Art, And Context, Miranda Gale

Theses & ETDs

The philosophy of food is an expansive topic, but most people recognize it for its contributions to ethics rather than aesthetics. Examining the potential categorization of food as art leads to a fuller understanding of art on its own terms, especially within a twentieth/twenty-first century, post-Duchampian context. Conceptual art in particular builds on a framework that espouses the concept as a medium, rejects a purely visual aesthetic, and blurs lines of form and content. Emphasizing context, conceptual artists--some of whom work with food as a medium--reject most of the categories that exclude food from art. In sketching a philosophically-informed art …

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Audible Sound Treatment Of Chlorella Vulgaris Beijerinck (1890) Has No Effect On Growth Rate, Nathan Burnaman Jan 2018

Audible Sound Treatment Of Chlorella Vulgaris Beijerinck (1890) Has No Effect On Growth Rate, Nathan Burnaman

Theses & ETDs

Microalgae, a group of single celled or simple multicellular organisms responsible for a significant portion of the world’s primary productivity, have the potential to be utilized for sustainable and potentially remediative production of fuel, food, and medicine. Recent experiments have shown that treating microalgae with sound can enhance growth and lipid production. Chlorella vulgaris, a microalga of interest in multiple industries, was cultured with continuous single frequency sound stimulation at 2200 Hz to observe changes in growth rates. Sound-treated alga cultures showed no significant difference in growth compared to control cultures. The literature on microalgal applications, as well as sound …

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Novel Antibiotic Design: Synthesis And Optimization Of N-Heterocyclic Oxazolidinones, David J. Bruch Jan 2018

Novel Antibiotic Design: Synthesis And Optimization Of N-Heterocyclic Oxazolidinones, David J. Bruch

Theses & ETDs

Over the past few decades there have been very few breakthrough discoveries in the field of antibiotics. Designing an effective drug is not a simple task, and requires a long and grueling selection and optimization process. The analogue approach is a very effective method in drug design and was applied to the N-aryl-oxazolidinone moiety in an effort to explore the structure activity relationship among this series. Dr. Tucker proposed a heterocyclic aromatic species as a substituent for placement at the N-aryl site of the oxazolidinone moiety. Specifically, we hoped to replace the N-phenyl “B-ring” in the active linezolid oxazolidinone core …

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“Together For Liberation, Together For Our Nation”: An Examination Of Feminist Literary Practice’S Navigation Of Contested Membership In Palestinian Nationalist Discourse, Leen Alfatafta Jan 2018

“Together For Liberation, Together For Our Nation”: An Examination Of Feminist Literary Practice’S Navigation Of Contested Membership In Palestinian Nationalist Discourse, Leen Alfatafta

Theses & ETDs

The purpose of this study is to understand how feminist literary practice can navigate contested membership within Palestinian Nationalist discourse. In particular, I am interested in the way the content of Sawt-Al-Nisa (Voice of Women) magazine responds to the questioning of Palestinian women’s nationalist credentials during the Second Intifada on the basis of their lack of participation in the militarized resistance. Employing qualitative content analysis, I examine the ways Palestinian women imagine themselves as nationalist actors, how self-definition tools are employed to navigate contested membership, and whether that can qualify as resistance from the margins. Two themes emerged from the …

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To Vaccinate Or Not To Vaccinate: An Overview Of The Immune Response And The Vaccine Debate, Noriám Roman Jan 2018

To Vaccinate Or Not To Vaccinate: An Overview Of The Immune Response And The Vaccine Debate, Noriám Roman

Theses & ETDs

Immunizations have had an impact on public health. They have decreased the morbidity and mortality of various diseases. Immunizations act on innate immunity to activate the adaptive immune system and create immune memory. Although immunizations have clearly decreased the incidence of disease, some people who question the safety and efficacy of vaccines. I discuss the various aspects of the immune system, explored the different types of immunizations that are available, and analyzed the anti-vaccine debate. After weighing benefits and harms, it can be concluded that vaccines are safe for most people. There are a few exceptions where some patients have …

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Prey Selection Of Octopus Vulgaris Cuvier 1797: An Analysis Of Individuals And Their Cohabitants In Cayos Cochinos, Honduras, 2017, Meaghan Klos Jan 2018

Prey Selection Of Octopus Vulgaris Cuvier 1797: An Analysis Of Individuals And Their Cohabitants In Cayos Cochinos, Honduras, 2017, Meaghan Klos

Theses & ETDs

Octopus vulgaris occupies a critical role in its ecosystem, as it is an important food source, ecosystem engineer, and a generalist predator. Understanding diet, especially diet shifts, is crucial to make predictions about ecosystem health. In addition to diet, octopuses can have a range of cohabiters in their dens that scavenge from the midden, the remains of the octopus’ consumed prey returned to the den. The dynamics surrounding why cohabiters exist at some dens and not others are not completely understood. Octopuses on a relatively healthy reef located at Cayos Cochinos, Honduras, were studied. Prey remains were retrieved from octopus …

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Bridging Academia And Management In Conservation Genetics: The Case Of The Florida Duskywing Skipper (Ephyriades Brunnea), Megan Elizabeth Barkdull Jan 2018

Bridging Academia And Management In Conservation Genetics: The Case Of The Florida Duskywing Skipper (Ephyriades Brunnea), Megan Elizabeth Barkdull

Theses & ETDs

By fragmenting natural habitats and reducing population sizes of many organisms planetwide, humans have disrupted normal evolutionary processes. Conservation geneticists use molecular markers to study the impact of habitat fragmentation and destruction on the genetic structure of species, searching for signatures of inbreeding and loss of genetic diversity in order to better manage imperiled species. In this study, I attempted to develop microsatellite markers for the Florida Duskywing skipper (Ephyriades brunnea), a vulnerable skipper butterfly that is a specialist in pine rocklands habitat, a globally-imperiled ecosystem type. Development and testing of these microsatellite markers is ongoing. To guide future analyses, …

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Wolbachia Detection In The Tawny-Edged Skipper (Polites Themistocles), Gabriella Elmir Jan 2018

Wolbachia Detection In The Tawny-Edged Skipper (Polites Themistocles), Gabriella Elmir

Theses & ETDs

In this study, I screened a Tawny-edged Skipper (Polites themistocles) population of 26 individuals collected from northeastern South Dakota for Wolbachia. Wolbachia is an endosymbiotic bacteria that negatively affects host reproduction. PCR products for Wolbachia 16S and arthropod 28S were prepared and gel electrophoresis was used to detect Wolbachia infection in the population. This study found that 96% of the Tawnyedged Skipper population presented with Wolbachia. This supports the literature that Wolbachia is prevalent in insect species. I then selected five P. themistocles (Poth) individuals from the population and performed sequence analysis using an established multilocus sequence typing (MLST) protocol …

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Striving To Be Sehat: An Ethnographic Investigation Of Indonesian Medical Practices, Trevor Johnson Jan 2018

Striving To Be Sehat: An Ethnographic Investigation Of Indonesian Medical Practices, Trevor Johnson

Theses & ETDs

There is evidence of a particular practice of medical syncretism in the contemporary medical practices of those in the central Javanese province/city of Yogyakarta, Indonesia. Individuals were often found to have combined ideas of traditional Javanese practice and ideas of cosmology as a dualistic means of determining healthcare choices in relation to the Indonesian national healthcare system established in 2014. This provides insights into how these choices, that at may seem counter to contemporary trends at first glance, are active, conscious understandings of how best to live a healthy lifestyle as informed by a large local, and national history.

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The New College Arboretum: A Site-Specific Analysis Of Trees In An Urban Landscape, Malcolm Wells Jan 2018

The New College Arboretum: A Site-Specific Analysis Of Trees In An Urban Landscape, Malcolm Wells

Theses & ETDs

Trees play key roles in moderating negative impacts of anthropogenic climate change and global urbanization. Their unique physiology and anatomy critically influence natural systems, facilitating life and playing an integral role in the health and functionality of urban landscapes. The arboreal diversity and sheer number of trees present on the New College of Florida campus begs the question: Why isn’t the school an arboretum? In this thesis, I explore this inquiry by investigating the botanical diversity of New College, and statistically comparing characteristics of New College with existing arboreta. By articulating the number of tree species present on the New …

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Reef Safe Sunscreens Are Not Less Toxic Than Standard Sunscreens To The Fertilization And Larval Survival Of Lytechinus Variegatus, Paul Marick Jan 2018

Reef Safe Sunscreens Are Not Less Toxic Than Standard Sunscreens To The Fertilization And Larval Survival Of Lytechinus Variegatus, Paul Marick

Theses & ETDs

Sunscreen and other skin care products are pollutants that enter the water via the skin of swimmers. “Reef safe” sunscreen is claimed by manufacturers to be safer for marine organisms. Reef safe sunscreens often lack known dangerous ingredients such as oxybenzone and nano-scale metal oxides. The purpose of this research is to establish if reef safe sunscreen is better for marine life by raising Lytechinus variegatus from gamete to embryo in three concentrations (0.0001%, 0.001%, and 0.01% by weight value) of both reef safe and standard sunscreens. There was no difference in the LC50 numbers of the reef safe and …

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A Comparative Analysis: Recapture Rates Of The Blacktip Shark (Carcharhinus Limbatus) In The Noaa Gulfspan Survey (2000-2016) And The Terra Ceia Bay And Sarasota Bay, Florida Study (2017), Justina Mei-Jing Bernard Jan 2018

A Comparative Analysis: Recapture Rates Of The Blacktip Shark (Carcharhinus Limbatus) In The Noaa Gulfspan Survey (2000-2016) And The Terra Ceia Bay And Sarasota Bay, Florida Study (2017), Justina Mei-Jing Bernard

Theses & ETDs

Elasmobranchs, known as the cartilaginous fishes and including sharks, skates, and rays, play a critical role in maintaining a healthy ecosystem. Through tag-recapture studies, many factors that influence the population model for elasmobranch populations can be investigated to monitor the health of a fish stock. A triple tagging method was used, where the tertiary tag was a triangular notch punched in the first dorsal fin of the shark to provide indication for whether it was part of the 2017 in the case of tag shedding. This study didn’t find a significant difference between the recapture rates of blacktip sharks captured …

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You’Ve Got To Be Joking: An English Translation Of The Philogelos, Annie Mccabe Jan 2018

You’Ve Got To Be Joking: An English Translation Of The Philogelos, Annie Mccabe

Theses & ETDs

The first ‘joke book’ was the Ancient Greek text Philogelos. It was composed in fourth century Rome, circulated over the course of several centuries, and remains largely intact in the modern day. As a peripheral text, it has been the subject of only sporadic research, translation, commentary and analysis until the later half of the twentieth century. This thesis offers a complete English translation of the Greek text with brief contextual and cultural commentary, as well as a historical overview of Greek and Roman humorous expression. The subject matter of the Philogelos bears resemblance to the social comedy of Greek …

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The Radical And Mystical Space Of Marginalized Motherhood: Reimagining And Redefining The Immaculata And Multi-Media Installation, Body With A Heart Shaped Face, Bree Nieves Jan 2018

The Radical And Mystical Space Of Marginalized Motherhood: Reimagining And Redefining The Immaculata And Multi-Media Installation, Body With A Heart Shaped Face, Bree Nieves

Theses & ETDs

This thesis analyzes representations of and ideals about motherhood using Patricia Hill Collin’s theories about the “Hegemonic Domain,” one of four interrelated domains of power in society. Motherhood is both a constructed pedagogical system and a place for radical love and creativity. In this thesis, I focus on the Christian history of motherhood and analyze the authority that Church fathers give to the Virgin Mary through biblical references and images. I focus on the misreading of biblical scripture and argue for the necessity to reimagine and rework time-worn constructs of motherhood. I also discuss the Genesis account, Christian biological assumptions …

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Sarasota Bay: A Newly Defined Nursery For Blacktip Sharks (Carcharhinus Limbatus) On The Gulf Coast Of Florida, Lukas Heath Jan 2018

Sarasota Bay: A Newly Defined Nursery For Blacktip Sharks (Carcharhinus Limbatus) On The Gulf Coast Of Florida, Lukas Heath

Theses & ETDs

Nursery areas provide juvenile sharks with abundant prey and protection from predators. Defining these Essential Fish Habitats is an integral component of effective species management and conservation plans. The blacktip shark (Carcharhinus limbatus) is a species of major economic importance in the Gulf of Mexico. The Gulf coast represents nearly continuous habitat for this species, but parturition is thought to occur in discrete nursery areas. Sarasota Bay, on the Gulf coast of Florida, is a 56-mile-long urbanized coastal lagoon system, comprised of five embayments, joined by the Intracoastal Waterway and separated from the Gulf of Mexico by a complex of …

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Characterizing The Bacterial Microbiome Of Corals Resilient To Climate Change And Ocean Acidification In St. John, Us Virgin Islands, Constance Sartor Jan 2018

Characterizing The Bacterial Microbiome Of Corals Resilient To Climate Change And Ocean Acidification In St. John, Us Virgin Islands, Constance Sartor

Theses & ETDs

The health of coral reefs is in decline worldwide and scientists are investigating assisted evolution as a tool for coral restoration. Assisted evolution allows scientists to enhance naturally occurring traits within corals through methods including selective breeding and manipulation of microbial communities. Unique mangrove communities in Hurricane Hole, U.S. Virgin Islands host corals that exhibit resistance to both thermal stress and ocean acidification. Through molecular sequencing of the microbial communities of these resistant corals, the factors that influence this resiliency may be identified and used for restoration via assisted evolution. Samples from three coral species—Colpophyllia natans, Diploria labyrinthiformis, and Orbicella …

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Paws Off! The Ethics And Behavioral Effects Of Feline Onychectomy, Stacia Mintner Jan 2018

Paws Off! The Ethics And Behavioral Effects Of Feline Onychectomy, Stacia Mintner

Theses & ETDs

Onychectomy is currently a highly debated topic in veterinary medicine. The practice of declawing domesticated house cats has been a common one over time, however, current studies have shown that the amputation of part of the paw can cause debilitating physical and psychological pain from side effects of the procedure. These side effects have been shown to alter cats’ behavior, causing them to act “inappropriately” at home. There is a need within veterinary medicine for more data on the outcomes of, and attitudes toward declawing. By furthering research in onychectomy, cats can be better understood medically and psychologically, ultimately leading …

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Lessons From Flowers For Dan Dan: Developing Interactive Drama For Virtual Reality And Measuring The Emotional Effects Of Voice Recognition, Hunter Osking Jan 2018

Lessons From Flowers For Dan Dan: Developing Interactive Drama For Virtual Reality And Measuring The Emotional Effects Of Voice Recognition, Hunter Osking

Theses & ETDs

This thesis is split into two sections. The first section focuses on the development of the game Flowers for Dan dan. It’s split into three chapters - the first of which works uses academic papers to contextualize Flowers for Dan dan’s place within the video game industry, as well as its place within the field of game design research. The second chapter is written as a deliberately informal story and tells a history of the development process of the game. The third chapter discusses the lessons learned in the development process of Flowers for Dan dan. The second section of …

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Key West After Hurricane Irma: An Exploration Of Selfefficacy, Post-Traumatic Growth, And Evacuation Behaviors, Savannah Flak Jan 2018

Key West After Hurricane Irma: An Exploration Of Selfefficacy, Post-Traumatic Growth, And Evacuation Behaviors, Savannah Flak

Theses & ETDs

The current study sought to explore the relationships between hurricane personal and coping self-efficacies, post-traumatic growth, Hurricane Irma evacuation decisions, and the likelihood of evacuating a future hurricane along with other demographic characteristics. This investigation is important because these factors were examined within the unique context of Key West, where the geographical constraints of the Florida Keys make evacuation more difficult than most hurricane-prone regions. Key West residents were recruited in person to take an online survey approximately four months after Hurricane Irma made landfall. Results indicated that an individual’s belief in their ability to prepare for a hurricane was …

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Power Is Sexy; Climate Change Is Not: A Look Into How Green Energies Can Mitigate Climate Change While Allowing Growth, Daviana King Jan 2018

Power Is Sexy; Climate Change Is Not: A Look Into How Green Energies Can Mitigate Climate Change While Allowing Growth, Daviana King

Theses & ETDs

The thesis research investigates finding out how first climate change works and influences the globe, how that influences human activities and economies, and then how best markets can respond to climate change with focus on green energy. Research shows that (1) green technology in much of its production of energy has been in the small scale compared to fossil fuels but have grown to become effective sources of energy, (2) government support by subsidy, education, regulation, or guidance is necessary to getting the energy to be implemented and cost-effective, (3) many programs have risen in the US and globally and …

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Redivivus Destruction & Renewal, Rebecca Miles Jan 2018

Redivivus Destruction & Renewal, Rebecca Miles

Theses & ETDs

Redivivus is an intricate installation set hundreds of years in the future that questions our role as humans within the spiraling cycle of life. A fictional character named Syl inhabits this future world, collects all the items displayed in the room and creates drawings of what she sees. This future is set within a post-apocalyptic timeline, with endless indications of hope and wonder depicted throughout the installation, evoking a powerful dichotomy between destruction and renewal. Biblical references, futuristic video games such as Fallout 3 and Horizon Zero Dawn, as well as the novel Dune by Frank Herbert informed my process. …

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