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Articles 601 - 630 of 61515
Full-Text Articles in Entire DC Network
The Abundance Of Nitrogen Fixing, Nitrifying, Denitrifying And Ammonifying Bacteria In The Soil Of Tropical Rainforests And Oil Palm Plantations In Jambi, Zulfarina, Iman Rusmana, Nisa Rachmania Mubarik, Dwi Andreas Santosa
The Abundance Of Nitrogen Fixing, Nitrifying, Denitrifying And Ammonifying Bacteria In The Soil Of Tropical Rainforests And Oil Palm Plantations In Jambi, Zulfarina, Iman Rusmana, Nisa Rachmania Mubarik, Dwi Andreas Santosa
Makara Journal of Science
Alterations in the use of land for oil palm plantations can change the domination and activity of soil bacteria. More specifically, alteration in soil microbial communities can directly affect soil ecosystem functioning, particularly with respect to carbon and nitrogen cycles. Nitrogen can be a limiting nutrient, and the availability of nitrogen in the soil environment becomes a major factor in controlling the production of biomass. This research project aimed at studying the abundance of nitrogen-fixing, nitrogen-oxidizing, nitrogen-reducing, and ammonifying bacteria based on their functional genes in the tropical rain forests of Taman Nasional Bukit Duabelas (TNBD) and the oil palm …
Identification Of A Major Quantitative Trait Locus For Grain Weight In Rice Using Microsatellite Marker, Mariana Susilowati, Hajrial Aswidinnoor, Wening Enggarini, Kurniawan Rudi Trijatmiko
Identification Of A Major Quantitative Trait Locus For Grain Weight In Rice Using Microsatellite Marker, Mariana Susilowati, Hajrial Aswidinnoor, Wening Enggarini, Kurniawan Rudi Trijatmiko
Makara Journal of Science
Rice is one of the major staple foods in the world, especially in Asia. Improving yield potential of superior cultivars is important to meeting the demand for rice production, which is increasing due to human population increase, climate change, and degradation of agricultural resources, such as land and water. In this study, a BC3F2 population developed from an intraspecific cross between Ciherang and a new plant type line (B11143D) was used in a quantitative trait locus (QTL) analysis. Ciherang is a high yielding rice cultivar with good grain quality which has been planted in 37% of the irrigated rice area …
One-Way Coupled Hydroelastic Analysis Of Aluminum Wedge Under Slamming, Mallikarjun Kalluru
One-Way Coupled Hydroelastic Analysis Of Aluminum Wedge Under Slamming, Mallikarjun Kalluru
LSU New Orleans Theses and Dissertations
The concept of using aluminum as the primary construction material for high speed ships and the hydroelastic behavior of the structure is widely gaining importance as a significant research topic in naval architecture. Aluminum is lighter than steel and hence can be predominantly used in high speed crafts which experiences significant slamming. This thesis work is focused on wedge shaped models. Free fall wedge impact is studied and a FORTRAN 90 computer program is developed to estimate the structural response of the wedge experiencing slamming by the use of matrix methods, finite element techniques and Newmark-Beta numerical time integration methods. …
Locating Unmanned Aerial Vehicles (Uavs), Ali Ghubaish
Locating Unmanned Aerial Vehicles (Uavs), Ali Ghubaish
McKelvey School of Engineering Graduate Student Theses & Dissertations
Despite the popularity and usefulness of Unmanned Aerial Vehicles (UAVs) or drones, they are not allowed to fly in some areas without prior permission from the Federal Aviation Administration (FAA). However, many incidents of UAVs breaching such restrictions have been reported. A UAV location system can help the law enforcement to be alerted and can prevent UAVs breaching any restricted area without permission. This master thesis proposes a UAV location system where each UAV has a unique identification tag. The method consists of two stages: distance and location estimation. We compared distance estimation using three different methods: Time of Arrival …
No Beginning And No End, Ryan Arthur Florey
No Beginning And No End, Ryan Arthur Florey
Theses
This thesis describes the realization of my art, created with the intention to project into a plastic medium the story of an ancient artifact, a weather-beaten stone, or a plant responding to stress in its environment. It describes the evolution of my inspiration and thought over the course of my graduate career, as well as, the various ancient cultures, artifacts, and traditions of making that I have found to be rooted in similar inspiration, and which have helped to guide the progression of my works.
Virtual Reality As A Training Tool To Treat Physical Inactivity In Children, Adam W. Kiefer, David Pincus, Michael J. Richardson, Gregory D. Myer
Virtual Reality As A Training Tool To Treat Physical Inactivity In Children, Adam W. Kiefer, David Pincus, Michael J. Richardson, Gregory D. Myer
Psychology Faculty Articles and Research
Lack of adequate physical activity in children is an epidemic that can result in obesity and other poor health outcomes across the lifespan. Physical activity interventions focused on motor skill competence continue to be developed, but some interventions, such as neuromuscular training (NMT), may be limited in how early they can be implemented due to dependence on the child’s level of cognitive and perceptual-motor development. Early implementation of motor-rich activities that support motor skill development in children is critical for the development of healthy levels of physical activity that carry through into adulthood. Virtual reality (VR) training may be beneficial …
Human Organs-On-A-Chip, Qin Jianhua, Zhang Min, Yu Hao, Li Zhongyu
Human Organs-On-A-Chip, Qin Jianhua, Zhang Min, Yu Hao, Li Zhongyu
Bulletin of Chinese Academy of Sciences (Chinese Version)
Human organs-on-a-chip is a newly emerging and frontier technology involving with different disciplines in recent years. It refers to a biomimetic microphysiological system created on a bioengineered microfluidic device, representing the organ-level functional units. It can recapitulate the physiologically relevant structures and functions of the organs, as well as the interaction between multiple organs as in vivo, thereby offering alternative models for predicting human responses to various drugs and environmental stimulus. Organs-on-a-chip technology has wide application potentials in many fields such as life science, drug discovery, personalized medicine, food/environment monitoring and biological defense et al. The emergence of this …
Electrochemical Sensing Of Neurotransmitters With High Temporal And Spatial Resolution, Zhang Yueyue, Zuo Xiaolei, Fan Chunhai
Electrochemical Sensing Of Neurotransmitters With High Temporal And Spatial Resolution, Zhang Yueyue, Zuo Xiaolei, Fan Chunhai
Bulletin of Chinese Academy of Sciences (Chinese Version)
Currently, real time monitoring the release of neurotransmitters from living cells with high temporal and spatial resolution remains challenging. In recent years, quantitative analysis of neurotransmitter releasing has been achieved by developing different electrochemical monitoring techniques, and cell release patterns have been investigated. In addition, monitoring with high resolution and sensitivity can be achieved by modifying the electrode surface or regulating the electrode dimensions. The combining of different monitoring techniques can further improve the monitoring capability. This paper reviews the mechanism of electrochemical detection of neurotransmitter, the development of microelectrode and nanoelectrode for neurotransmitter detection, the coupling of electrochemical technology …
Biosensors: 50 Years Development And Future Perspectives, Zhang Xian-En
Biosensors: 50 Years Development And Future Perspectives, Zhang Xian-En
Bulletin of Chinese Academy of Sciences (Chinese Version)
Biosensors are the devices made of biological elements and physical or chemical transducers for fast and specific determination of biological or related substances, of which is a typical convergence technology. Biosensors have broad application prospects in life science research, disease diagnosis and control, bioengineering process control, agriculture and food security, environmental quality monitoring, biosafety and biosecurity, etc. 50 years after its birth, biosensor technology has entered a new phase of vigorous development. The main driving forces are the introduction and implementation of the concepts of One-Health, Internet of Things, and big data. Research hot topics include wearable and portable biosensing …
Research Progress Of Bioinspired Smell And Taste Sensors, Wang Ping, Zhuang Liujing, Qin Zhen, Zhang Bin, Gao Keqiang
Research Progress Of Bioinspired Smell And Taste Sensors, Wang Ping, Zhuang Liujing, Qin Zhen, Zhang Bin, Gao Keqiang
Bulletin of Chinese Academy of Sciences (Chinese Version)
The mammalian olfactory and gustatory system is recognized as the most effective chemosensing systems due to their extraordinary ability in odor and tastant detection. Detection of odors has been applied to many real applications, such as quality control of food products, safety and security, environmental monitoring, medical diagnosis and so on. Here, we focus on the principle, basic composition, employed technology and application of electronic nose/electronic tongue, molecule and cell based olfactory/gustatory biosensors. We also introduce the research progress of a novel system which we called ' in vivo bioelectronic nose and tongue'. Finally, the further trend in this area …
Molecular Biosensors And Molecular Imaging In Cells, Wang Dianbing, Cui Zongqiang, Zhang Xian-En
Molecular Biosensors And Molecular Imaging In Cells, Wang Dianbing, Cui Zongqiang, Zhang Xian-En
Bulletin of Chinese Academy of Sciences (Chinese Version)
Molecular sensors are the biosensors made of macrobiomolecules. They are significant tools for real-time imaging of the molecular events in living cells and in vivo. Molecular beacons (MB), fluorescence/bioluminescence resonance energy transfer sensors (FRET/BRET) and molecular fluorescence complementation systems (BiFC, TriFC, etc.) are major types of molecular biosensors for live cell imaging applications. In this paper, the principles and characteristics of the molecular biosensors are described. The biosensors show their unique role in study of biomolecular interactions, localization, movement, and kinetics in living cells. The restrains, challenges, and future prospects of the molecular biosensors are also discussed. Because "Seeing …
Exploring Mindfulness Training Effects On Cognition And Psychological Wellbeing In Young And Older Adults, Rongxiang Tang
Exploring Mindfulness Training Effects On Cognition And Psychological Wellbeing In Young And Older Adults, Rongxiang Tang
Arts & Sciences Graduate Student Theses and Dissertations
Previous investigations have revealed a range of cognitive, physiological, and psychological benefits following mindfulness training in young, middle-aged, and older adults. The aim of this thesis was to provide new insights into three issues that have not been adequately addressed in the extant literature. First, the application and integration of mindfulness training within conventional educational contexts is limited. In study 1, I provide evidence of the utility and effectiveness of mindfulness training incorporated as a part of traditional college curriculum. Second, although mindfulness training has been shown to offset age-related cognitive declines, studies have yet to combine mindfulness training with …
Weighted Inequalities For Dyadic Operators Over Spaces Of Homogeneous Type, David Edward Weirich
Weighted Inequalities For Dyadic Operators Over Spaces Of Homogeneous Type, David Edward Weirich
Mathematics & Statistics ETDs
A so-called space of homogeneous type is a set equipped with a quasi-metric and a doubling measure. We give a survey of results spanning the last few decades concerning the geometric properties of such spaces, culminating in the description of a system of dyadic cubes in this setting whose properties mirror the more familiar dyadic lattices in R^n . We then use these cubes to prove a result pertaining to weighted inequality theory over such spaces. We develop a general method for extending Bellman function type arguments from the real line to spaces of homogeneous type. Finally, we uses this …
The Interaction Between Encapsulated Gd2o3 Particles And Polymeric Matrix: The Mechanism Of Fracture And X-Ray Attenuation Properties, Ly B. T. La, Christopher Leatherday, Peng Qin, Yee-Kwong Leong, Kevin Hayward, Bin Jiang, Lai-Chang Zhang
The Interaction Between Encapsulated Gd2o3 Particles And Polymeric Matrix: The Mechanism Of Fracture And X-Ray Attenuation Properties, Ly B. T. La, Christopher Leatherday, Peng Qin, Yee-Kwong Leong, Kevin Hayward, Bin Jiang, Lai-Chang Zhang
Research outputs 2014 to 2021
This work sheds light on the relationship between the quantities of synthesized core shell Gd2O3 added to epoxy matrix and the mechanical and X-ray attenuation properties of particulate epoxy composite. Then, an optimal geometric design of non-lead based X-ray protective material with light weight per volume unit is prepared. A plateau with 28–30 % increments in the value of fracture toughness (KIC ) is observed with a specific addition of 0.08 – 0.1 volume fraction (φS) of Gd2O3 particles in pure epoxy. The same quantity of particles also optimally raises …
Chaucer's Divided "I": Narrative Voice And Performance Dynamics In Late Fourteenth-Century English Literature, Isaac Mcduffie
Chaucer's Divided "I": Narrative Voice And Performance Dynamics In Late Fourteenth-Century English Literature, Isaac Mcduffie
LSU Doctoral Dissertations
Throughout the twentieth century, various Chaucer scholars have worked to achieve the now-consensus opinion that Chaucer wrote his works expecting them to be both listened to within a group setting and read privately in an individual setting. These scholars have either assumed or expressly stated that the experience of listening to and reading Chaucer’s works would have been the same. This dissertation argues otherwise. I show that in the late fourteenth century, English vernacular was only starting to be understood as a written language, and this peculiar circumstance created tensions between both listening and reading modes of reception. Such tensions …
Biblical Patriarchy In Doctrine And Practice: An Analysis Of Evangelical Christian Homeschooling, Tonya Vander
Biblical Patriarchy In Doctrine And Practice: An Analysis Of Evangelical Christian Homeschooling, Tonya Vander
Intermountain West Journal of Religious Studies
“I spent most of my days while homeschooling trying to figure out how to be a better person, how to be more perfect, how to be a better homeschooler, a better dishwasher, a better everything,” Janine, a mother, reflected on her homeschooling years. In the United States, homeschooled children made up three percent of the population in the 2011-2012 school year. In addition, two-thirds of all homeschooling families were concerned about the integration of religion in their children’s education as a core reason for choosing homeschool over public school. Some Evangelical Christian homeschoolers (ECHS) responded to such concerns by tailoring …
Continuity In The Face Of Social Change: Demographic Shifts And The United Methodist Church’S Institutional Conservatism On Sexuality, Scott Vehstedt
Continuity In The Face Of Social Change: Demographic Shifts And The United Methodist Church’S Institutional Conservatism On Sexuality, Scott Vehstedt
Intermountain West Journal of Religious Studies
In the wake of the Gay Liberation movement that flourished during the 1960s and 1970s, Protestant churches were forced to grapple with crafting policies toward homosexuality. As gay men and women demanded acceptance in the public sphere, so too did they demand it in matters of theology. Although sexual preference was historically a matter that the United Methodist Church (UMC) left to parishioners and their local clergy, the rise of LGBTQ activism brought non-heteronormative sexuality into the mainstream of politics, culture, and religion.1 Throughout the 1970s Protestant denominations, including the UMC, were forced to address this cultural shift through policy
The Shape Of Water, Ken Derry
The Shape Of Water, Ken Derry
Journal of Religion & Film
This is a film review of The Shape of Water (2017), directed by Guillermo del Toro.
Haiyun Tci Practice Series (1): From Input To Output: Introduction To Teaching With Comprehensible Input, Haiyun Lu
Chinese Language Teaching Methodology and Technology
Dr. Stephen Krashen’ work in the second language acquisition has been influencing how language is taught in a classroom in the last 40 years. The comprehension based instructions from Teaching Proficiency Through Reading and Storytelling (TPRS), Movie Talk (MT), Embedded Reading and Story Listening to Total Physical Response (TPR) and Natural Approach, etc., have fundamentally challenged a traditional grammar-driver language class’s status quo, and impacted the real classroom applications; hence, the result of proficiency.
A Study Of The Nature And Impact Of Shona And Chinese Rhyming Words/Phrases, Herbert Mushangwe, Muchinei Musona
A Study Of The Nature And Impact Of Shona And Chinese Rhyming Words/Phrases, Herbert Mushangwe, Muchinei Musona
Chinese Language Teaching Methodology and Technology
Pairs of rhyming words/phrases in Chinese and Shona were investigated in an attempt to bring out the effect of such words to the teaching and learning of Chinese language. The pairs were grouped in accordance to their effects and nature of relationship. The selection of the words was based on samples obtained during classroom teaching. Students’ reaction to Chinese words automatically tells the story of the effect of such rhyming words. For instance, for embarrassing rhyming words learners naturally shy out to pronounce the words or they laugh instead of imitating the teacher. It is clear that the study of …
Ecological And Evolutionary Genomics: Differential Variability Of Gene Expression And Fine-Scale Adaptive Divergence Among Microhabitats Of Large Fundulus Heteroclitus Populations, Dominique Neitzel Wagner
Ecological And Evolutionary Genomics: Differential Variability Of Gene Expression And Fine-Scale Adaptive Divergence Among Microhabitats Of Large Fundulus Heteroclitus Populations, Dominique Neitzel Wagner
Open Access Dissertations
Adaptive evolution on ecological timescales shapes communities. However, adaptation among environments relies on isolation or large selection coefficients that exceed migration effects. This reliance is tempered if adaptation from standing genetic variation is polygenic – does not depend on one allele completely replacing another but instead requires small allele frequency changes at many loci. Thus, whether individuals can evolve adaptation to fine-scale habitat variation is not resolved. To investigate this process, the gene expression and genetic divergence of a teleost fish, Fundulus heteroclitus, was explored. Among nearby (< 200m) microhabitats in three separate saltmarshes, and over the course of a season (spring to fall), thousands of single-nucleotide polymorphisms (SNPs) were analyzed. Among these SNPs, 1.3-2.3% have large and highly significant differences among microhabitats (mean FST = 0.15; false discovery rate (FDR) ≤ 1%), and 1.8-5.7% are significantly different between seasons (mean FST = 0.077 to 0.597; FDR ≤ 1%). The divergence among microhabitats and between seasons for these SNPs is larger than that among populations, exceeds neutral expectation, and indicates surprising population structure among microhabitats. These data suggest that polygenic selection is surprisingly effective in altering allele frequencies over very small geographic distances.
Mems Scale Integrated Lab-On-A-Chip System For Detection And Analysis Of Human Tumor Cells, Rajapaksha Waththegedara Randil Lalinda Gajasinghe
Mems Scale Integrated Lab-On-A-Chip System For Detection And Analysis Of Human Tumor Cells, Rajapaksha Waththegedara Randil Lalinda Gajasinghe
Open Access Dissertations
Detection and analysis of circulating tumor cells (CTCs) present in human blood is gaining attention as a reliable technique for obtaining information that are vital for diagnostic and research procedures. Only a limited number of semi-automated commercial tools capable of single cell detection and analysis exist; they are expensive and bulky instruments that require highly trained operators. Micro-electromechanical systems (MEMS) are ideal for the application due to their inherent advantages. A method for immobilizing target CTCs at MEMS scale regions of interest was developed. Developed MEMS device utilized superparamagnetic micro beads and micro electromagnets to immobilize MCF-7 cells flowing in …
Environmental Durability And Degradation Of Fiber-Reinforced Bismaleimide/Quartz Composite For Aircraft Radome Applications, Luis A. Rodriguez
Environmental Durability And Degradation Of Fiber-Reinforced Bismaleimide/Quartz Composite For Aircraft Radome Applications, Luis A. Rodriguez
Open Access Dissertations
Bismaleimide (BMI) is a polymeric thermoset material that is suitable for high temperature structural and electrical applications, particularly when used as a matrix in fiber-reinforced composites for radar-protecting structures (radomes) on commercial and military aircraft. In this role, BMI’s high service temperature and high strength-to-weight ratio are of significant importance in supersonic aircraft. A main disadvantage of this material is the propensity to absorb environmental and aircraft related fluids in many applications, even in hot, dry environments. Moisture absorption has been known to be a deleterious factor in the service life of aircraft structures, especially in the case of dielectric …
Anomalous Structures In Oceanic Turbulence: Dynamics, Energetics, Transport, Mykhailo Rudko
Anomalous Structures In Oceanic Turbulence: Dynamics, Energetics, Transport, Mykhailo Rudko
Open Access Dissertations
In this thesis, anomalous structures of oceanic mesoscale turbulence, as exemplified by coherent vortices and zonally-elongated transient flows (ZELTs), are considered. All simulations of mesoscale turbulence flows are performed in a two-layer, quasi-geostrophic model. The first chapter explores stability of and transport by baroclinic vortices on the β-plane. The study adapts a wave-mean flow formalism and examines interactions between the axisymmetric flow (“the vortex”) and residuals (“the waves”). Unlike baroclinically unstable vortices on the f-plane, such vortices on the β-plane can be also vulnerable to barotropic instability as revealed by the globally integrated energy balance analysis. The spatial structure of …
The Role Of Carbon Capture And Storage Electricity In Attaining 1.5 And 2°C, Adriano Vinca, Marianna Rottoli, Giacomo Marangoni, Massimo Tavoni
The Role Of Carbon Capture And Storage Electricity In Attaining 1.5 And 2°C, Adriano Vinca, Marianna Rottoli, Giacomo Marangoni, Massimo Tavoni
Fondazione Eni Enrico Mattei Working Papers
The climate targets defined under the Paris agreement of limiting global temperature increase below 1.5 or 2°C require massive deployment of low-carbon options in the energy mix, which is currently dominated by fossil fuels. Scenarios suggest that Carbon Capture and Storage (CCS) might play a central role in this transformation, but CCS deployment is stagnating and doubts remain about its techno-economic feasibility. In this article, we carry out a throughout assessment of the role of CCS electricity for a variety of temperature targets, from 1.5 to above 4°C, with particular attention to the lower end of this range. We collect …
To Go Or Not To Go: Migration Alleviates Climate Damages Even For Those Who Stay Behind, Soheil Shayegh, Greg P. Casey
To Go Or Not To Go: Migration Alleviates Climate Damages Even For Those Who Stay Behind, Soheil Shayegh, Greg P. Casey
Fondazione Eni Enrico Mattei Working Papers
We examine the effect of climate change on fertility rates and human capital accumulation in developing countries, focusing on the instrumental role of migration. In particular, we investigate how climate-induced migration in developing countries will affect those who do not migrate. Holding all else constant, climate shocks raise the return to acquiring skills, because skilled individuals compared to unskilled ones have greater opportunity to migrate after the shock. In response to this change in incentives, parents choose to invest more in education and have less children, a process known as the ‘quantity-quality’ trade-off. These effects partially offset the damages of …
Linking Heterogeneous Climate Policies (Consistent With The Paris Agreement), Michael A. Mehling, Gilbert E. Metcalf, Robert N. Stavins
Linking Heterogeneous Climate Policies (Consistent With The Paris Agreement), Michael A. Mehling, Gilbert E. Metcalf, Robert N. Stavins
Fondazione Eni Enrico Mattei Working Papers
The Paris Agreement has achieved one of two key necessary conditions for ultimate success – a broad base of participation among the countries of the world. But another key necessary condition has yet to be achieved – adequate collective ambition of the individual nationally determined contributions. How can the climate negotiators provide a structure that will include incentives to increase ambition over time? An important part of the answer can be international linkage of regional, national, and subnational policies, that is, formal recognition of emission reductions undertaken in another jurisdiction for the purpose of meeting a Party’s own mitigation objectives. …
Redesign Of The Bridgewater State University Website, Jordan Rossetti
Redesign Of The Bridgewater State University Website, Jordan Rossetti
Honors Program Theses and Projects
In this thesis, I studied human-computer interaction (HCI), analyzed the usability of the Bridgewater State University website, and redesigned it guided by HCI principles and user experiments. A goal of the project is to apply suggestions from HCI research to the usability analysis of an existing website. I redesigned the website based on this analysis and by conducting user experiments compare the redesign against the existing BSU website. With this project, I redesigned a website that demonstrably improves users’ success at information seeking and navigation while reducing frustration and hassle compared to the current Bridgewater State University (BSU) website. This …
2017 Fall Engr333 Project Final Report (Section A), Trevor Nyeholt, Francis Kapesa, Justin Thalmayer, Erik Karlson, Kyle Van Veen, Kirk Brink, Steven Tarske, Brent Homan, Nathan De Haan, Paul Bootsma, Megan Anders, Philip Holmes, Melanie Fox, Noah Pirrotta, Abigail Berkompas, Ben Wellman, Halley Press, Christopher Greaves, Josh Templeman, Tim Bosch, Jake Zandstra, Hendrik Vermeulen
2017 Fall Engr333 Project Final Report (Section A), Trevor Nyeholt, Francis Kapesa, Justin Thalmayer, Erik Karlson, Kyle Van Veen, Kirk Brink, Steven Tarske, Brent Homan, Nathan De Haan, Paul Bootsma, Megan Anders, Philip Holmes, Melanie Fox, Noah Pirrotta, Abigail Berkompas, Ben Wellman, Halley Press, Christopher Greaves, Josh Templeman, Tim Bosch, Jake Zandstra, Hendrik Vermeulen
ENGR 333
Calvin College currently spends $836,000 annually on natural gas consumption. The engineering 333-A class was tasked with the question, "What would it take for Calvin College to save $75,000 per year on natural gas costs". The class was split into five groups (Boilers, Dorms and Dining Hall, Academic Buildings, Finance, and PE Complex) to research possible areas of savings. Through the class' research, it was determined that Calvin College has the potential savings of $87,000 a year through the reduction of natural gas usage.
Changes In Enzyme Structural Dynamics Studied By Hydrogen Exchange-Mass Spectrometry: Ligand Binding Effects Or Catalytically Relevant Motions?, Courtney S Fast, Siavash Vahidi, Lars Konermann
Changes In Enzyme Structural Dynamics Studied By Hydrogen Exchange-Mass Spectrometry: Ligand Binding Effects Or Catalytically Relevant Motions?, Courtney S Fast, Siavash Vahidi, Lars Konermann
Chemistry Publications
It is believed that enzyme catalysis is facilitated by conformational dynamics of the protein scaffold that surrounds the active site, yet the exact nature of catalytically relevant protein motions remains largely unknown. Hydrogen/deuterium exchange (HDX) mass spectrometry (MS) reports on backbone H-bond fluctuations. HDX/MS thus represents a promising avenue for probing the relationship between enzyme dynamics and catalysis. A seemingly straightforward strategy for such studies involves comparative measurements during substrate turnover and in the resting state. We examined the feasibility of this approach using rabbit muscle pyruvate kinase (rM1-PK) which catalyzes the conversion of phosphoenolpyruvate and Mg-ADP to pyruvate and …