Digital Commons Network^™

Development Of C5ar-Targeted Nanoparticles For Delivery Of Vaccines, Shailendra Bharadwaj Tallapaka Venkata Sesha

Theses & Dissertations

Since the early attempts of Benjamin Jesty at inducing immunity against smallpox and the pioneering work of Edward Jenner, vaccination has been, and continues to remain, the principal method of protection from diseases. However, most of the successful vaccines have been against pathogens that do not have major mechanisms to evade the immune system. So far, many life-threatening diseases like hepatitis C, HIV infection, malaria etc., have been resistant to existing vaccination strategies. Thus, there is an urgent need to develop new vaccination strategies that can generate long-lived protective immunity against such pathogens.

The purpose of this thesis is to …

Development Of Cxcr4 Inhibitors For Topical Treatment Of Psoriasis, Suthida Boonsith

Theses & Dissertations

Psoriasis is a chronic inflammatory skin disease that is often associated with systemic comorbidities and impaired skin barrier function. There are several treatment options including topical treatment with immunosuppressants, phototherapy, and systemic therapies using small molecules and biological agents. However, none of them can be regarded as a perfect solution due to their toxicity during chronic use. Recently, the CXCR4/SDF-1 axis was found to play an important role in the pathogenesis of psoriasis. AMD3100, a small molecule CXCR4 antagonist can selectively bind to CXCR4 and inhibit skin inflammation, reduce angiogenesis and infiltration of inflammatory cells. Unfortunately, AMD3100 can only be …

Evaluation Of Real-Time Feedback To Train Caregivers To Conduct Early Intensive Behavioral Interventions, Amanda L. Gibson

Theses & Dissertations

Mounting empirical support for early intensive behavioral intervention (EIBI) has increased demand for these types of treatments for children diagnosed with autism spectrum disorder (ASD). Many caregivers are now learning EIBI techniques and becoming active agents in their child’s ASD treatment. Behavioral skills training (BST) has been frequently used to teach individuals to perform a variety of skills correctly, including discrete-trial instruction (DTI; Lafasakis & Sturmey, 2007). In this study, caregivers were trained to conduct a DTI procedure. A single-component BST method (i.e., real-time feedback) was examined. A concurrent, multiple baseline across caregivers design was used to demonstrate experimental control. …

Bio Brain, Tracey Bentley

UNH Today Archive

No abstract provided.

Redirection Of The Immune Response To Staphylococcus Aureus Biofilm Infection, Anna G. Staudacher

Theses & Dissertations

Staphylococcus aureus (S. aureus) is a leading cause of community- and healthcare-associated infections and has a propensity to form biofilms. Biofilm infections are recalcitrant to host immune-mediated clearance as well as antibiotics, making them exceptionally difficult to eradicate. The biofilm environment has been shown to skew the host immune response towards an anti-inflammatory phenotype, characterized by alternatively activated macrophages, recruitment of myeloid-derived suppressor cells (MDSCs), and minimal neutrophil and T cell infiltrates. Our laboratory has attempted to redirect the host immune response towards one that would favor bacterial clearance by employing strategies to augment pro-inflammatory mechanisms. One such …

Molecular Mechanisms Of C-Terminal Eps15 Homology Domain Containing (Ehd) Protein Function, Kriti Bahl

Theses & Dissertations

Endocytic trafficking is not only an essential process for the maintenance of cellular homeostasis but also plays a vital role in regulating diverse cellular processes such as signaling, migration and cell division. The C-terminal Eps 15 Homology Domain proteins (EHD1-4) play pivotal roles in regulating distinct steps of endocytic trafficking. Among the EHDs, EHD2 is disparate both in terms of sequence homology (70%) and its subcellular localization at the caveolae. The crystal structure of EHD2 has been solved and it contains an unstructured loop consisting of two proline-phenylalanine (PF) motifs: KPFRKLNPF. However, the other paralogs EHD1, …

Understanding The Chondrogenic Potential Of Articular Chondrocytes, Krishna Sarma

Theses & Dissertations

Articular cartilage is a smooth, visco-elastic, aneural, avascular tissue made of water, an exquisitely organized framework of proteoglycans, glycosaminoglycans, and collagen fibrils and articular chondrocytes. It’s beautiful organization and composition provide it with the flexibility and strength to cover, protect and lubricate the ends of long bones in a diarthrodial joint. Cartilage homeostasis relies on articular chondrocytes to translate the mechanical forces of daily activity into efficient remodeling of the extracellular matrix. Age, joint injury, or other insulting factors can progressively incapacitate articular chondrocytes, resulting in cartilage lesions that devolve to degenerative joint disease. Therefore, the central idea explored in …

Bedtime Fading With Response Cost For Treatment Of Sleep Disturbances In Children With Autism Spectrum Disorder, Sela Ann Sanberg

Theses & Dissertations

As many as 82% of children with Autism Spectrum Disorders (ASD) experience numerous chronic sleep-related problems and at a much higher frequency and severity than their typically developing peers. Behavioral treatments are considered best practice and first-line treatment to address sleep problems. These treatments tend to address one specific sleep-related behavior at a time. Bedtime Fading with Response Cost (BFRC) is a promising intervention that targets a multitude of sleep problems concurrently and has yet to be replicated by more than one investigative team in the home setting with children on the autism spectrum. This study evaluated the effectiveness of …

The Bg News August 18, 2017, Bowling Green State University

BG News (Student Newspaper)

The BGSU campus student newspaper. August 18, 2017. Volume 97-Issue 1.

Stress-Related Growth In Two Challenging Conditions, Iva Solcova, Peter Tavel

Journal of Human Performance in Extreme Environments

In the last few years, attention has been given not only to negative impacts of stressful conditions but also to possible positive outcomes. The present study was devoted to personal growth in two challenging conditions in two samples of participants coming from different nations and different cultural backgrounds. The study extends existing knowledge by more intimate insight into the different facets of social and cognitive/affective personal growth. The experience of personal growth was individualized as far as the number of changes, their intensity, and facets/items in which the changes occurred.

Least affected was the spirituality area. The reason is probably …

Dnmt3a Haploinsufficiency Provokes Hematologic Malignancy Of B-Lymphoid, T-Lymphoid, And Myeloid Lineage In Mice, Garland Michael Upchurch

Theses & Dissertations

DNA methyltransferase 3A (DNMT3A) is a master epigenetic regulator of benign and malignant hematopoiesis. To dissect the biological consequences of homozygous and heterozygous Dnmt3a inactivation in malignant hematopoiesis, we generated Dnmt3a homozygous null (Dnmt3a^Δ/Δ) and Dnmt3a heterozygous (Dnmt3a^+/–) mice and compared the presentations of hematologic malignancies between cohorts. Bi-allelic inactivation of Dnmt3a results in the presentation of mature lymphoid neoplasms resembling chronic lymphocytic leukemia (CLL; B220⁺CD19⁺CD5⁺; 88% penetrance (37/42)) and CD8+ peripheral T-cell lymphoma (PTCL; TCRβ⁺CD3⁺CD8⁺CD4^—; 40% penetrance (17/42)). …

School Counselor Transgender Advocacy Development: An Exploratory Qualitative Study, Jack F. Jacobs

Graduate Theses, Dissertations, and Capstones

School counselors have an opportunity to develop as an advocate for their transgender students who face many adversities in life. However, there exists a paucity of research specific to school counselor advocacy and no literature specific to school counselors developing as advocates for transgender students. In an attempt to address the gaps in the literature, the purpose of this qualitative research was to explore how three school counselors in a large urban school district in the southeast United States participating in an inquiry group developed as transgender advocates while completing a passion project. The conceptual framework components of the study …

Control Of Glyphosate-Resistant Common Ragweed (Ambrosia Artemisiifolia L.) In Glufosinate-Resistant Soybean [Glycine Max (L.) Merr], Ethann R. Barnes, Stevan Z. Knezevic, Peter H. Sikkema, John L. Lindquist, Amit J. Jhala

Department of Agronomy and Horticulture: Faculty Publications

Common ragweed emerges early in the season in Nebraska, USA and is competitive with soybean; therefore, preplant herbicides are important for effective control. Glyphosate has been used as a preplant control option; however, confirmation of glyphosate-resistant (GR) common ragweed in Nebraska necessitates evaluating other herbicide options. The objectives of this study were to (1) evaluate the efficacy of preplant (PP) herbicides followed by (fb) glufosinate alone or in tank-mixture with imazethapyr, acetochlor, or S-metolachlor applied post-emergence (POST) for control of GR common ragweed in glufosinate-resistant soybean; (2) their effect on common ragweed density, biomass, and soybean yield; and (3) the …

The Margination And Transport Of Particles In Blood Flow, Erik James Carboni

Doctoral Dissertations

Archival abstract submitted.

Application Of Variational Principle To Form Reduced Fluid-Structure Interaction Models In Bifurcated Networks, Alexander S. Liberson, Yashar Seyed Vahedein, David A. Borkholder

Articles

Reduced fluid-structure interaction models have received a considerable attention in recent years being the key component of hemodynamic modeling. A variety of models applying to specific physiological components such as arterial, venous and cerebrospinal fluid (CSF) circulatory systems have been developed based on different approaches. The purpose of this paper is to apply the general approach based on Hamilton’s variational principle to create a model for a viscous Newtonian Fluid - Structure Interaction (FSI) in a compliant bifurcated network. This approach provides the background for a correct formulation of reduced FSI models with an account for arbitrary nonlinear visco-elastic properties …

The Roles Of Ecdysoneless In Endoplasmic Reticulum Stress And Oncogenesis, Appolinaire A. Olou

Theses & Dissertations

Tumor cells are well known to exhibit ER stress as a result of their altered environment characterized by redox and calcium imbalance, deregulation of protein synthesis to meet their oncogenic demands, and decreased vascularization associated with nutrient limitation and hypoxia, all of which are conducive of ER stress. Accordingly, markers of ER stress signaling response are up-regulated in various cancers. The outcome of ER stress signaling response varies from survival and adaption to apoptosis. Although pro-apoptotic ER stress signaling molecules are up-regulated in cancer, the mechanisms by which cancer cells seem refractory to, or evade, their apoptotic signaling are not …

The Role Of The ʿUlamā’ In The Thoughts Of ʿAbd Al-Fattāḥ Abū Ghudda, Emad Hamdeh

Publications

Despite his influence and contribution to scholarship in the modern Muslim world, the life and works of ʿAbd al-Fattāḥ Abū Ghudda are underrepresented in Western academic literature. This article is a first initiative at a broader assessment and contextualization of Abū Ghudda’s life and thoughts. I present a picture of a scholar who sought to represent traditional Islam in its most unpopular moments. In particular, I examine Abū Ghudda’s prevailing thoughts and opinions concerning “proper” scholarship and demonstrate how the role of the ʿulamā’ in the thoughts of Abu Ghudda is primarily a continuation of a scholarly tradition rather than …

Rethinking Urban Green Infrastructure As A Means To Promote Avian Conservation, Allen Lau

Master's Projects and Capstones

There is an under-recognized potential for cities to use urban green infrastructure to contribute to avian biodiversity conservation. At the global scale, climate change and growing urbanization are primary global drivers leading to decline and homogenization in world bird populations. Birds are fundamental and intricate species in ecosystems, and even in urban areas, act as indicator and regulator species contributing to healthy ecosystem function. While many cities have recognized the economic and social benefits associated with green spaces, such as the vast benefits ecosystem services provide to the urban dweller, the use of green spaces to concurrently contribute to avian …

Outcomes In 370 Patients With Mantle Cell Lymphoma Treated With Ibrutinib: A Pooled Analysis From Three Open-Label Studies, S Rule, M Dreyling, A Goy, G Hess, Et Al

Peninsula Medical School

Ibrutinib is highly active in treating mantle cell lymphoma (MCL), an aggressive B-cell lymphoma. We pooled data from three ibrutinib studies to explore the impact of baseline patient characteristics on treatment response. Patients with relapsed/refractory MCL (n = 370) treated with ibrutinib had an objective response rate (ORR) of 66% (20% complete response; 46% partial response); median duration of response (DOR), progression-free survival (PFS) and overall survival (OS) were 18·6, 12·8 and 25·0 months, respectively. Univariate analyses showed patients with one versus >one prior line of therapy had longer OS. Multivariate analyses identified that one prior line of therapy affected …

Effects Of Ocean Acidification On The Shells Of Four Mediterranean Gastropod Species Near A Co 2 Seep, Ashley Duquette, James B. Mcclintock, Charles D. Amsler, Alberto Pérez-Huerta, Marco Milazzo, Jason M. Hall-Spencer

School of Biological and Marine Sciences

No abstract provided.

Sensitive Droplet Digital Pcr Method For Detection Of Tert Promoter Mutations In Cell Free Dna From Patients With Metastatic Melanoma, Ashleigh C. Mcevoy, Leslie Calapre, Michelle Pereira, Tindaro M. Giardina, Cleo Robinson, Muhammad A. Khattak, Tarek M. Meniawy, Antonia L. Pritchard, Nicholas K. Hayward, Benhur Amanuel, Michael J. Millward, Mel R. Ziman Dr, Elin S. Gray

Research outputs 2014 to 2021

Background:

Currently mainly BRAF mutant circulating tumor DNA (ctDNA) is utilized to monitor patients with melanoma. TERT promoter mutations are common in various cancers and found in up to 70 % of melanomas, including half of BRAF wildtype cases. Therefore, a sensitive method for detection of TERT promoter mutations would increase the number of patients that could be monitored through ctDNA analysis.

Methods:

A droplet digital PCR (ddPCR) assay was designed for the concurrent detection of chr5:1,295,228 C > T and chr5:1,295,250 C > T TERT promoter mutations. The assay was validated using 39 melanoma cell lines and 22 matched plasma and …

A Comparison Of Soils And Their Associated Microbial Communities As Affected By Sugarcane Cultivation, Adam Francis Bigott

LSU Master's Theses

In Louisiana, sugarcane has been grown in the same soils for over 200 years. A phenomenon wherein soils with a long-term sugarcane cropping history produce decreased yields compared to adjacent land without a recent history of sugarcane cultivation has been documented in multiple sugarcane growing regions. Research in both Louisiana and internationally has shown positive plant growth responses when soils with a long-term cultivation history are sterilized or treated with selective biocides, suggesting there is a biological component to the underlying soil health issue. In this study, soil microbial ecology was compared for paired sites with short and long-term sugarcane …

Copyright Easements, Jason Mazzone

Akron Law Review

When authors assign the copyright in their work to publishers, some productive uses of the work are impeded. The author loses opportunities to use or to authorize others to use the work unless the publisher consents; the publisher does not permit all uses of the work that the author would like or that would benefit a consuming audience. Copyright easements can solve the problem. Under a system of copyright easements, an easement holder would have designated rights in a creative work that would permit uses of the work that would ordinarily require permission of the copyright owner. If the author …

Reconsidering Experimental Use, Rochelle Cooper Dreyfuss

Akron Law Review

In the years since the Supreme Court began to narrow the scope of patentable subject matter, uncertainties in the law have had a deleterious impact on several important innovation sectors, including, in particular, the life sciences industry. There are now initiatives to expand patentable subject matter legislatively. In this article, I suggest that the Supreme Court’s jurisprudence is an outgrowth of the concern that patents on fundamental discoveries impede scientific research. To deal with that issue, any measure to expand the subject matter of patenting should be coupled with a parallel expansion of defenses to infringement liability, including the restoration …

Nagel, Jackson, And Physicalism, Sarah Huizar

Prologue: A First-Year Writing Journal

No abstract provided.

Star Trek: A Guide To The Soul And Virtuous Action, Melissa Cherry

Prologue: A First-Year Writing Journal

No abstract provided.

Engaging Medical Students In Problem-Based Search And Study Of The Biomedical Literature., Zhiyong Han, Samantha L Margulies, Divya Kurian, Joshua M Jabaut, Akshita Mehta, Ramzi Dudum, Huberta Koudoro, Ria S Roberts, Jay Lee, Jonathan Li, Hieu T Nguyen, Mark Elliott

Biochemistry and Molecular Medicine Faculty Publications

No abstract provided.

Thyroid Function And Ischemic Heart Disease: A Mendelian Randomization Study, Jie V. Zhao, C. Mary Schooling

Publications and Research

To clarify the role of thyroid function in ischemic heart disease (IHD) we assessed IHD risk and risk factors according to genetically predicted thyroid stimulating hormone (TSH), free thyroxine (FT4) and thyroid peroxidase antibody (TPOAb) positivity. Separate-sample instrumental variable analysis with genetic instruments (Mendelian randomization) was used in an extensively genotyped case (n = 64,374)-control (n = 130,681) study, CARDIoGRAMplusC4D. Associations with lipids, diabetes and adiposity were assessed using the Global Lipids Genetics Consortium Results (n = 196,475), the DIAbetes Genetics Replication And Meta-analysis case (n = 34,380)-control (n = 114,981) study, and the Genetic Investigation of ANthropometric Traits (body …

Process Considerations For Protein A Affinity Capture, Virus Inactivation, And Linked Polishing Steps In Multi-Column Continuous Purification Of Monoclonal Antibodies, Robert Mierendorf, Tom Van Oosbree, Anthony Grabski, William Wessel, Emily Schirmer

Integrated Continuous Biomanufacturing III

Multi-column continuous chromatography (MCC) offers significant economic advantages over traditional batch methods for purification of monoclonal antibodies (mAbs), including increased resin capacity utilization, smaller columns, reduced buffer consumption, and faster process time. The Protein A capture step is a primary target to apply MCC due to its high cost, which is driven even higher as improvements in upstream processing have produced a steady increase in mAb titers. In this study we consider the key factors in designing an MCC process that optimizes productivity. Process design begins with determination of the feed duration, which is governed by the mAb titer, column …

In Re: Wellbutrin Xl Antitrust

2017 Decisions

USDC for the Eastern District of Pennsylvania