Digital Commons Network^™

Co-Independent Modification Of K+ Channels By Tricarbonyldichlororuthenium(Ii) Dimer (Corm-2), Guido Gessner, Nirakar Sahoo, Sandip M. Swain, Gianna Hirth, Roland Schönherr, Ralf Mede, Matthias Westerhausen, Hans Henning Brewitz, Pascal Heimer, Diana Imhof

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Although toxic when inhaled in high concentrations, the gas carbon monoxide (CO) is endogenously produced in mammals, and various beneficial effects are reported. For potential medicinal applications and studying the molecular processes underlying the pharmacological action of CO, so-called CO-releasing molecules (CORMs), such as tricabonyldichlororuthenium(II) dimer (CORM-2), have been developed and widely used. Yet, it is not readily discriminated whether an observed effect of a CORM is caused by the released CO gas, the CORM itself, or any of its intermediate or final breakdown products. Focusing on Ca2+- and voltage-dependent K+ channels (KCa1.1) and voltage-gated K+ channels (Kv1.5, Kv11.1) relevant …

Distal Humeral Fractures-Current Concepts, James C. Beazley, Njalalle Baraza, Robert Jordan, Chetan S. Modi

General Surgery, East Africa

Background: Distal humerus fractures constitute 2% of all fractures in the adult population. Although historically, these injuries have been treated non-operatively, advances in implant design and surgical technique have led to improved outcomes following operative fixation.

Methods: A literature search was performed and the authors' personal experiences are reported.

Results: This review has discussed the anatomy, classifications, treatment options and surgical techniques in relation to the management of distal humeral fractures. In addition, we have discussed controversial areas including the choice of surgical approach, plate orientation, transposition of the ulnar nerve and the role of elbow arthroplasty.

Conclusion: Distal humeral …

Cryptic Diversity And Discordance In Single‐Locus Species Delimitation Methods Within Horned Lizards (Phrynosomatidae: Phrynosoma), Christopher Blair, Robert W. Bryson Jr.

Publications and Research

Biodiversity reduction and loss continues to progress at an alarming rate, and thus there is widespread interest in utilizing rapid and efficient methods for quantifying and delimiting taxonomic diversity. Single-locus species-delimitation methods have become popular, in part due to the adoption of the DNA barcoding paradigm. These techniques can be broadly classified into tree-based and distance-based methods depending on whether species are delimited based on a constructed genealogy. Although the relative performance of these methods has been tested repeatedly with simulations, additional studies are needed to assess congruence with empirical data. We compiled a large data set of mitochondrial ND4 …

Outlier Identification In Radiation Therapy Knowledge-Based Planning: A Study Of Pelvic Cases., Yang Sheng, Yaorong Ge, Lulin Yuan, Taoran Li, Fang-Fang Yin, Qingrong Jackie Wu

Department of Radiation Oncology Faculty Papers

PURPOSE: The purpose of this study was to apply statistical metrics to identify outliers and to investigate the impact of outliers on knowledge-based planning in radiation therapy of pelvic cases. We also aimed to develop a systematic workflow for identifying and analyzing geometric and dosimetric outliers.

METHODS: Four groups (G1-G4) of pelvic plans were sampled in this study. These include the following three groups of clinical IMRT cases: G1 (37 prostate cases), G2 (37 prostate plus lymph node cases) and G3 (37 prostate bed cases). Cases in G4 were planned in accordance with dynamic-arc radiation therapy procedure and include 10 …

Using Competition Assays To Quantitatively Model Cooperative Binding By Transcription Factors And Other Ligands., Jacob Peacock, James B Jaynes

Department of Biochemistry and Molecular Biology Faculty Papers

BACKGROUND: The affinities of DNA binding proteins for target sites can be used to model the regulation of gene expression. These proteins can bind to DNA cooperatively, strongly impacting their affinity and specificity. However, current methods for measuring cooperativity do not provide the means to accurately predict binding behavior over a wide range of concentrations.

METHODS: We use standard computational and mathematical methods, and develop novel methods as described in Results.

RESULTS: We explore some complexities of cooperative binding, and develop an improved method for relating in vitro measurements to in vivo function, based on ternary complex formation. We derive …

Evaluating Long-Term Direct And Correlated Selection Response In White Plymouth Rock Chickens Selected For High Or Low 8-Week Body Weight, Sylvia J. Harrison

Department of Animal Science: Dissertations, Theses, and Student Research

The increasing demand for poultry meat has led animal breeders to engage in artificial selection of chickens as a way to increase the productivity of poultry. Long-term experiments have been designed to measure rates of genetic response to a trait under selection, and correlated traits, as well as gauge possible selection limits.

Two studies were conducted to evaluate response to selection for body weight (BW) in chickens. The chickens were selected for high or low BW at 8 weeks of age. Those that met the criterion were selected as parents for the subsequent generation. In the first study the population …

Scaling Dissolved Nutrient Removal In River Networks: A Comparative Modeling Investigation, Sheng Ye, Alexander J. Reisinger, Jennifer L. Tank, Michelle A. Baker, Robert O. Hall, Emma J. Rosi, Murugesu Sivapalan

Biology Faculty Publications

Along the river network, water, sediment, and nutrients are transported, cycled, and altered by coupled hydrological and biogeochemical processes. Our current understanding of the rates and processes controlling the cycling and removal of dissolved inorganic nutrients in river networks is limited due to a lack of empirical measurements in large, (nonwadeable), rivers. The goal of this paper was to develop a coupled hydrological and biogeochemical process model to simulate nutrient uptake at the network scale during summer base flow conditions. The model was parameterized with literature values from headwater streams, and empirical measurements made in 15 rivers with varying hydrological, …

Calibration-Free Spectrophotometric Measurements Of Carbonate Saturation States In Seawater, Erin E. Cuyler

USF Tampa Graduate Theses and Dissertations

This work describes efforts to improve methodologies and instrumentation for investigation of the marine CO₂ system. In the first section of my thesis, a method was developed that provides simple, calibration-free measurements of seawater carbonate saturation states (Ω_spec) based solely on the use of a laboratory spectrophotometer. Measurements of pH are made in paired optical cells, one with and one without added nitric acid. The amount of added nitric acid is determined through the direct proportionality between nitrate concentration and UV absorbance. After an initial calibration, the method is calibration-free and requires no volumetric or gravimetric analyses …

Measuring Trust In Post-Communist States: Making The Case For Particularized Trust., Nicole M. Ford

USF Tampa Graduate Theses and Dissertations

While the literature on democracy and its relationship to trust provides little consensus regarding the role of trust, researchers have emphasized the importance of generalized trust over particularized in relation to democracy. This research marks a departure from this consensus, and exposes the neglected role of personal relationships in fostering successful democracy.

One of the key measurements of democracy in a country is social trust. There are three forms of trust: generalized, particularized and institutional. Previously, the measurement of social trust focused on the importance of generalized trust, that is, trust in those we do not know (Putnam, 1993; Fukuyama, …

Design, Fabrication, And Characterization Of Hg2 Sensor Based On Graphite Oxide And Metallic Nanoclusters, Abdul Rehman Said Rehman

Electrical Engineering Theses

It has been previously demonstrated that improving the detection of mercury (Hg²⁺) in water is of significant importance since the Hg²⁺ has detrimental effects on human health and could lead to toxicity, especially in utero. One way this could be achieved is through the design of highly sensitive and selective sensors for Hg²⁺ contents in water.

In this work, it is hypothesized that sensors with silver nanoclusters enhance the sensitivity of the fabricated sensor and possess the highest selectivity to the Hg²⁺ detected in this study. In order to verify this hypothesis, sensors with silver …

Modelling And Simulation Of Hydrogen Production Via Membrane Reactor, Aya Abdel-Hamid Smail Mourad

Chemical and Petroleum Engineering Theses

A membrane reactor is a promising device to produce pure hydrogen and enrich CO₂ from syngas. A simulation study of a double tubular catalytic membrane reactor for the water-gas shift reaction (WGS) under steady-state operation is presented in this work. The membrane consists of a dense Pd layer (selective to H₂) deposited on a porous glass cylinder support. The reaction side was filled with a commercial iron-chromium oxide catalyst, designed as Girdler G-3. The mass of the catalyst was 12.1 g and the height of the catalyst bed was 8 cm. The WGS model was carried out …

Phosphoproteomics Profiling Of Nonsmall Cell Lung Cancer Cells Treated With A Novel Phosphatase Activator, Danica Wiredja, Marzieh Ayati, Sahar Mazhar, Jaya Sangodkar, Sean Maxwell, Daniela Schlatzer, Goutham Narla, Mehmet Koyutürk, Mark R. Chance

Computer Science Faculty Publications

Activation of protein phosphatase 2A (PP2A) is a promising anti-cancer therapeutic strategy, as this tumor suppressor has the ability to coordinately downregulate multiple pathways involved in the regulation of cellular growth and proliferation. In order to understand the systems-level perturbations mediated by PP2A activation, we carried out mass spectrometry-based phosphoproteomic analysis of two KRAS mutated non-small cell lung cancer (NSCLC) cell lines (A549 and H358) treated with a novel Small Molecule Activator of PP2A (SMAP). Overall, this permitted quantification of differential signaling across over 1,600 phosphoproteins and 3,000 phosphosites. Kinase activity assessment and pathway enrichment implicated collective downregulation of RAS …

Workplace Trust As A Mechanism Of Employee (Dis)Advantage: The Case Of Employee Socioeconomic Status, Marko Pitesa, Stefan Thau, Madan M. Pillutla

Research Collection Lee Kong Chian School Of Business

Early work on trust in social science highlighted how the lack of trust between individuals can cause social division, contribute to social stratification, and reduce economic opportunities for people from all social groups. We integrate this work with organizational research on antecedents of trust to generate predictions explaining when and why low employee socioeconomic status (SES) can be a barrier to trust. We discuss how this process can impair the success of both organizations as well as their lower-SES employees. We present a model, and data, suggesting that lower-SES employees will be both more distrusted as well as more distrustful …

Synthetic Food Additives In Selected Beverages Sold In The Uae, Aysha Mohammed Aldhaheri

Biology Theses

The consumption of beverages is increasing around the world with the globalization of the food industry. Synthetic additives can cause a potential risk to human health when they are excessively consumed. Thus, it is important that the level of synthetic food additives do not exceed the CODEX standards and similar to authorized levels in United Arab Emirates (UAE) by Emirates Authority for Standards and Metrology (ESMA). The objectives of this thesis were to determine and analyze selected food additives in non-alcoholic beverages and to ensure that the quantified results comply with CODEX standards. The present research aimed at screening the …

Northwest Forest Plan The First 20 Years (1994-2013): Watershed Condition Status And Trend, Stephanie A. Miller, Sean N. Gordon, Peter Eldred, Ronald M. Beloin, Steve Wilcox, Mark Raggon, Heidi Andersen, Ariel Muldoon

Institute for Sustainable Solutions Publications and Presentations

We used two data sets to evaluate stream and upslope/riparian condition for sixth-field watersheds in each aquatic province within the Northwest Forest Plan (NWFP) area. The stream evaluation was based on stream sampling data collected from 2002 to the 2013 (214 watersheds) as part of an eight year repeating (rotating) sample design. We are currently halfway through our second rotation of stream sampling, and have repeated 110 watersheds since the second rotation began in 2009. The analysis presented in this report uses roughly half the number of watersheds as was originally intended by the sample design since re-visitation will not …

The Role Of Elites In The Formation Of National Identities: The Case Of Montenegro, Muhammed F. Erdem

USF Tampa Graduate Theses and Dissertations

This study aims to answer two interlinked central questions with respect to Montenegrins’ divide over statehood and identity: Why and how Montenegrins, whom were once called ‘the purest and the best of Serbs’, sought to end their century-long common state experience with Serbia and instead establish their own nation-state in 2006, and what explains the rise of Montenegrin national identity and its transformation into nationalism? In attempting to answer these questions, it traces the historical development of Montenegrin national thought dating back to the early 20^th century when Montenegro was annexed by the Kingdom of Serbs, Croats, and Slovenes. …

Framing Genetically-Modified Foods Communication In China: A Content Analysis Of News Coverage Of People’S Daily And Southern Metropolis, Linqi Lu

USF Tampa Graduate Theses and Dissertations

Grounded in framing theory, this thesis presents a qualitative content analysis of newspaper coverage of genetically modified foods by two Chinese newspapers- People’s Daily and Southern Metropolis, in 2000-2017. The political, economic, public opinion and legal implications involved have made the reports of genetically modified (GM) foods present different framing, themes, and positions between People’s Daily and Southern Metropolis. This study aims to examine the various frames used in the coverage of GM foods in two major Chinese newspapers that operate within different media frameworks.

Results of the content analysis illustrated that significant differences existed in the newspapers in …

Thermodynamic And Kinetic Aspects Of Hen Egg White Lysozyme Amyloid Assembly, Tatiana Miti

USF Tampa Graduate Theses and Dissertations

Deposition of protein fibers with a characteristic cross-β sheet structure is the molecular marker associated with human disorders ranging from Alzheimer's disease to type II diabetes and spongiform encephalopathy. Given the large number of non-disease related proteins and peptides that have been shown to form amyloid fibrils in vitro, it has been suggested that amyloid fibril formation represents a generic protein phase transition. In the last two decades it has become clear that the same protein/peptide can assemble into distinct morphologically and structurally amyloid aggregates depending on the solution conditions. Moreover, recent studies have shown that the early stage, oligomeric …

Genetic Identification And Population Characteristics Of Deep-Sea Cephalopod Species In The Gulf Of Mexico And Northwestern Atlantic Ocean, Amanda Sosnowski

USF Tampa Graduate Theses and Dissertations

Nearly all deep-sea cephalopod life history studies have been completed by examination of specimens collected in the wild. Much of this work is like piecing together a puzzle; knowledge of the life history of many species remains fragmented and hence, taxonomically and phylogenetically confused. Molecular approaches and sequencing technologies are powerful tools for deciphering wild-type cephalopod life history and population dynamics. Use of molecular markers offers additional certainty for identifying specimens damaged during deep-sea collections and can elucidate often cryptic, intra- and interspecific diversity. The research presented in this study assessed broad genetic patterns of biodiversity in deep-sea cephalopods from …

Decomposing The “Tacit Knowledge Problem:” Codification Of Knowledge And Access In Crispr Gene-Editing, Neil Thompson, Samantha Zyontz

Faculty Scholarship

No abstract provided.

Reach-Scale River Metabolism Across Contrasting Sub-Catchment Geologies: Effect Of Light And Hydrology, Lorenzo Rovelli, Karl M. Attard, Andrew Binley, Catherine M. Heppell, Henrik Stahl, Mark Trimmer, Ronnie N. Glud

All Works

© 2017 The Authors Limnology and Oceanography published by Wiley Periodicals, Inc. on behalf of Association for the Sciences of Limnology and Oceanography We investigated the seasonal dynamics of in-stream metabolism at the reach scale (∼ 150 m) of headwaters across contrasting geological sub-catchments: clay, Greensand, and Chalk of the upper River Avon (UK). Benthic metabolic activity was quantified by aquatic eddy co-variance while water column activity was assessed by bottle incubations. Seasonal dynamics across reaches were specific for the three types of geologies. During the spring, all reaches were net autotrophic, with rates of up to 290 mmol C …

The Influence Of Coring Method On The Preservation Of Sedimentary And Biogeochemical Features When Sampling Soft-Bottom, Shallow Coastal Environments, Andrew O.M. Mogg, Karl M. Attard, Henrik Stahl, Tim Brand, Robert Turnewitsch, Martin D.J. Sayer

All Works

© 2017 The Authors Limnology and Oceanography: Methods published by Wiley Periodicals, Inc. on behalf of Association for the Sciences of Limnology and Oceanography It is frequently assumed that taking samples of soft marine sediments using surface-based gravity coring equipment causes minimal disruption to their sedimentological, biogeochemical, and biological condition. This assumption was evaluated by examining the potential disturbances caused when obtaining soft-sediment samples either by SCUBA or Craib-coring, comparing sediment oxygen microprofiles, benthic oxygen flux rates and sediment solid phase analyses (chlorophyll a (Chl a), organic carbon, and porosity) between both methods and against reference values measured in situ …

Platelet Rich Plasma In Achilles Tendon Healing 2 (Path-2) Trial: Protocol For A Multicentre, Participant And Assessor-Blinded, Parallel-Group Randomised Clinical Trial Comparing Platelet-Rich Plasma (Prp) Injection Versus Placebo Injection For Achilles Tendon Rupture, J Alsousou, Dj Keene, Pa Hulley, P Harrison, Et Al

School of Health Professions

Background Achilles tendon injuries give rise to substantial long-lasting morbidity and pose considerable challenges for clinicians and patients during the lengthy healing period. Current treatment strategies struggle to curb the burden of this injury on health systems and society due to lengthy rehabilitation, work absence and reinjury risk. Platelet-rich plasma (PRP) is an autologous preparation that has been shown to improve the mechanobiological properties of tendons in laboratory and animal studies. The use of PRP in musculoskeletal injuries is on the increase despite the lack of adequately powered clinical studies. Methods and design This is a multicentre randomised controlled trial …

Β-Sitosterol Protects Against Cisplatin-Induced Nephrotoxicity Through Amelioration Of Oxidative Stress In Rats, Atheer Abbas Yaseen Al-Fatlawi, Aws Rassul Hussain Al-Salih

Muthanna Medical Journal

Kidney damage is a major concern related with Anti-malignancy drugs. It is well established that overproduction of reactive oxygen species plays vital role in progress of the pathogenesis of nephrotoxicity. The aim of this study is investigated the modulatory effect β-sitosterol (BT) on cisplatin (CP) that induced nephrotoxicity by targeting oxidative stress and biochemical parameters for kidney function markers in rats. Following a single dose of cisplatin (CP) an intraperitoneal (7.5 mg/kg BW) at seventh day group II, III and β-sitosterol (BT) 5 ml/kg was administered for 10 days group I,III, IV, after decapitation of the rats, trunk blood was …

Pharmaceutical And Natural (Exercise) Mechanisms To Mitigate The Negative Impact Of Ptsd And Chronic Stress On Synaptic Plasticity And Memory, Roxanne M. Miller

Theses and Dissertations

Synapses can be altered due to experiences in a process called synaptic plasticity, which causes memory formations. Synapses can be strengthened through methods known as long-term potentiation (LTP) or weakened through long-term depression (LTD). Stresses can cause changes by altering synapses through either LTP or LTD. Rats were used to study the effects of post-traumatic stress disorder (PTSD)-like symptoms and a prophylactic treatment using pharmaceuticals. The first model used was the single prolonged stress (SPS) with two weeks of chronic light, which was not as effective for causing changes in synaptic plasticity. The second model, seven days of social defeat …

A Metadata Reporting Framework (Frames) For Synthesis Of Ecohydrological Observations, Danielle S. Christianson, Charuleka Varadharajan, Bradley O. Christoffersen, Matteo Detto, Boris Faybishenko, Bruno O. Gimenez, Val Hendrix, Kolby J. Jardine, Robinson Negron-Juarez, Gilberto Z. Pastorello

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Metadata describe the ancillary information needed for data preservation and independent interpretation, comparison across heterogeneous datasets, and quality assessment and quality control (QA/QC). Environmental observations are vastly diverse in type and structure, can be taken across a wide range of spatiotemporal scales in a variety of measurement settings and approaches, and saved in multiple formats. Thus, well-organized, consistent metadata are required to produce usable data products from diverse environmental observations collected across field sites. However, existing metadata reporting protocols do not support the complex data synthesis and model-data integration needs of interdisciplinary earth system research. We developed a metadata reporting …

Mito-Apocynin Prevents Mitochondrial Dysfunction, Microglial Activation, Oxidative Damage, And Progressive Neurodegeneration In Mitopark Transgenic Mice, Monica Langley, Anamitra Ghosh, Adhithiya Charli, Souvarish Sarkar, Muhammet Ay, Jie Luo, Jacek Zielonka, Timothy Brenza, Brian Bennett, Huajun Jin, Shivani Ghaisas, Benjamin Schlichtmann, Dongsuk Kim, Vellareddy Anantharam, Arthi Kanthasamy, Balaji Narasimhan, Balaraman Kalyanaraman, Anumantha G. Kanthasamy

Physics Faculty Research and Publications

Aims: Parkinson's disease (PD) is a neurodegenerative disorder characterized by progressive motor deficits and degeneration of dopaminergic neurons. Caused by a number of genetic and environmental factors, mitochondrial dysfunction and oxidative stress play a role in neurodegeneration in PD. By selectively knocking out mitochondrial transcription factor A (TFAM) in dopaminergic neurons, the transgenic MitoPark mice recapitulate many signature features of the disease, including progressive motor deficits, neuronal loss, and protein inclusions. In the present study, we evaluated the neuroprotective efficacy of a novel mitochondrially targeted antioxidant, Mito-apocynin, in MitoPark mice and cell culture models of neuroinflammation and mitochondrial dysfunction.

Results: …

Age And Growth Of Bonefish, Albula Species Among Cuban Habitats, Jacob Joseph Rennert

Theses and Dissertations

Bonefish (Albula spp.) are a prized sportfish among avid anglers worldwide. Two morphologically indistinguishable species of bonefish (Albula vulpes and Albula goreensis) exist in the circumtropical waters of the western Atlantic. A. vulpes grows faster and reaches larger sizes in the Florida Keys than in the Bahamas and other insular regions, but the mechanisms driving this variation have not been identified. Cuba supports large populations of bonefish, but their age, growth rate, and reproductive biology has not previously been examined. I obtained specimens from fish markets and local research staff in 3 regions around the periphery of Cuba from November …

Increasing Habitat Complexity On Seawalls: Investigating Large‐ And Small‐Scale Effects On Fish Assemblages, Rl Morris, Mg Chapman, Lb Firth, Ra Coleman

School of Biological and Marine Sciences

The construction of artificial structures in the marine environment is increasing globally. Eco-engineering aims to mitigate the negative ecological impacts of built infrastructure through designing structures to be multifunctional, benefiting both humans and nature. To date, the focus of eco-engineering has largely been on benefits for benthic invertebrates and algae. Here, the potential effect of eco-engineered habitats designed for benthic species on fish was investigated. Eco-engineered habitats (“flowerpots”) were added to an intertidal seawall in Sydney Harbour, Australia. Responses of fish assemblages to the added habitats were quantified at two spatial scales; large (among seawalls) and small (within a seawall). …

Corporate Crisis Advertising: A Framework Examining The Use And Effects Of Corporate Advertising Before And After Crises, Benjamin Ho, Wonsun Shin, Augustine Pang

Research Collection Lee Kong Chian School Of Business

While corporate advertising has been widely studied as a promotional tool, few studies have examined how it can be used in a corporate crisis situation. In 2013, Kim proposed a conceptual framework for examining stakeholders’ evaluation of pre-crisis corporate advertising, using the inoculation and reactance theory. The framework, published in Journal of Marketing Communications, suggested that pre-crisis advertising can increase audience resistance towards negative news of an organization and decrease audience resistance towards future corporate advertisements from the organization. The present study expands on Kim’s work to develop the corporate crisis advertising (CCA) framework. In addition to the inoculation and …