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2017

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Pulmonary Lesion Texture Classification From Endobronchialultrasonogram Using Texture Analysis, Banphatree Khomkham Jan 2017

Pulmonary Lesion Texture Classification From Endobronchialultrasonogram Using Texture Analysis, Banphatree Khomkham

Chulalongkorn University Theses and Dissertations (Chula ETD)

This research aims to develop a method to help distinguish the appearance of pulmonary lesions from a high-frequency sound (Endobronchial Ultrasound—EBUS) image. According to medical information, the appearance of smooth or rough texture of a lesion can significantly indicate that it is malignant or benign. In this study, the features that are used in the classification are divided into 3 groups: group 1 consists of 22 standard features, group 2 consists of the proposed features extracted from the weighted sum of the upper and lower GLCM which consists 12 features, and group 3 is the combination of group 1 and …

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Mathematical Models For Treatment Time Of Pathogenic Infection In Blood After Antibiotics Administration, Panit Suavansri Jan 2017

Mathematical Models For Treatment Time Of Pathogenic Infection In Blood After Antibiotics Administration, Panit Suavansri

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objective in this thesis is to create a mathematical model to predict the period of time needed to clear out the pathogens from the bloodstream after the patient receives certain antibiotics. The result will lead to the time where the drug administration can be terminated. This research is to compute the densities of bacteria and malaria in bloodstream at any given time and to compare this level with the actual results of the patient's blood sampling, which was sent to the laboratory for investigation. The laboratory process of the blood sampling will usually take more than 2-3 days to …

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Revitalizing Tio Chew Chinese Cemetery Through A Design Of An Elderly Center, Khai Sin Lee Jan 2017

Revitalizing Tio Chew Chinese Cemetery Through A Design Of An Elderly Center, Khai Sin Lee

Chulalongkorn University Theses and Dissertations (Chula ETD)

Old cemeteries are often threatened by new urban development, which can result in them being closed, relocated or demolished. This thesis studies Tio Chew Cemetery in Bangkok as an alternative case based on its current unique use as a recreation area. Although without the threats of demolition, several on-site issues, both tangible and intangible, are facing Tio Chew Cemetery. To respond to these issues, a design of an elderly center is proposed to improve and revitalize the cemetery through the understanding of historical importance and contemporary design of new buildings in the old compound. This research applies a mixed methodology …

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Cloning And Characterisation Of Alternansucrase From Leuconostoc Citreum Abk-1 And Role Of Surface Aromatic Acids On Product Size, Karan Wangpaiboon Jan 2017

Cloning And Characterisation Of Alternansucrase From Leuconostoc Citreum Abk-1 And Role Of Surface Aromatic Acids On Product Size, Karan Wangpaiboon

Chulalongkorn University Theses and Dissertations (Chula ETD)

The alternansucrase (ALT, EC 2.4.1.140) catalyses transferring of glucose from sucrose to produce a polymer with α-1,6 and α- 1,3 linkages, so-called "alternan". The ALT-encoding gene from Leuconostoc citreum ABK-1 (WTalt) was successfully cloned and expressed in Escherichia coli BL21 (DE3). The two versions of truncated SH3-like domains, ∆3SHALT and ∆7SHALT, were successfully constructed. The WTALT, ∆3SHALT and ∆7SHALT possessed optimum temperature of 40 °C, while optimum pH of WTALT was at 5.0 but ∆3SHALT's and ∆7SHALT's were shifted to pH 4.0. Their activities could be enhanced by Mn²⁺. Kinetic studies of all ALTs were mainly responsible for transglycosylation activity …

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Expression And Characterization Of Α-Glucosidase From Weissella Confusa Bbk-1, Lalita Silapasom Jan 2017

Expression And Characterization Of Α-Glucosidase From Weissella Confusa Bbk-1, Lalita Silapasom

Chulalongkorn University Theses and Dissertations (Chula ETD)

Alpha-glucosidase [EC 3.2.1.20; α-D-glucoside glucohydrolase] is an exohydrolase which catalyzes non-reducing end of substrates to release D-glucose. In addition, alpha-glucosidase also displays a transferase activity, which bring about the formation of α-glucosylated compounds such as soluble starch and glycogen. This research aims to express and characterize α-glucosidase from Weissella confusa BBK-1 (WcAG). WcAG gene was expressed in modified pET28b vector. This gene contained an open reading frame of 1,775 bps. Optimum expression condition of WcAG was cultured in LB medium containing 1% (w/v) glucose and induced with 0.4 mM IPTG at 20°C for 20 h. The recombinant WcAG containing his-tag …

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Synthesis Of Fisetin Glycosides By Cyclodextrin Glycosyltransferase From Paenibacillus Sp. Rb01, Nattawadee Lorthongpanich Jan 2017

Synthesis Of Fisetin Glycosides By Cyclodextrin Glycosyltransferase From Paenibacillus Sp. Rb01, Nattawadee Lorthongpanich

Chulalongkorn University Theses and Dissertations (Chula ETD)

Fisetin is a flavonoid which was widely exerted in pharmacological industries. However, fisetin has low water solubility and unstable in aqueous solution. So the uses of fisetin have been limited. The purpose of this study was to increase water solubility of fisetin by transglycosylation reaction using cyclodextrin glycosyltransferase (CGTase) from Paenibacillus sp. RB01. The reaction had to perform in co-organic solvent system and DMSO at 40% (v/v) was proved to be the most suitable co-organic solvent since it provided the high enzyme stability and high production yield. Moreover, the types of glycosyl donor were also determined and β-cyclodextrin was found …

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Oxidative Responses In Transgenic 'Kdml105' Rice Oryza Sativa L. Overexpressing Oscam1-1 Against Drought Stress, Pun Sangchai Jan 2017

Oxidative Responses In Transgenic 'Kdml105' Rice Oryza Sativa L. Overexpressing Oscam1-1 Against Drought Stress, Pun Sangchai

Chulalongkorn University Theses and Dissertations (Chula ETD)

The OsCaM1-1 is a rice calmodulin protein which transduces Ca2+ signals to their target protein that is important to regulate cellular and physiological responses. Drought stress is the one of essential environmental stresses that affected the crop by changing in morphological, physiological, and biochemical direction tactics of plants. In this study, the effect of drought stress on growth and antioxidant system in the transgenic rice overexpressing OsCaM1-1 gene was investigated and compared to the control and wild type KDML 105. The overexpression of OsCaM1-1 in transgenic rice could increase plant tolerance to drought stress, the results showed the greater increase …

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Isolation And Characterization Of Triclocarban Degrading Bacteria As Plant Growth Promoter, Merry Krisdawati Sipahutar Jan 2017

Isolation And Characterization Of Triclocarban Degrading Bacteria As Plant Growth Promoter, Merry Krisdawati Sipahutar

Chulalongkorn University Theses and Dissertations (Chula ETD)

Triclocarban (TCC) is a widely used as antimicrobial agent in household products. High usage volume of TCC results in its accumulation in sludge and lead to contamination in environments. Application of biosolid in agricultural activities leads to contamination of TCC in agricultural areas. So. development of bioremediation technique for TCC is necessary to maintain quality of agricultural ecosystem. For that purpose, this research isolated 5 soil bacteria capable of TCC degradation and with plant-growth promoting (PGP) activities including Ochrobactrum sp. MC22, Ochrobactrum sp. MC35, Sphingobacterium sp. MC43, Pseudomonas sp. MS45, and Pseudomonas fluorescens MC46. Among them, Ochrobactrum sp. MC22 and …

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Effects Of Curcumin-Loaded Bacterial Cellulose Films On Cancer Cells, Chayut Subtaweesin Jan 2017

Effects Of Curcumin-Loaded Bacterial Cellulose Films On Cancer Cells, Chayut Subtaweesin

Chulalongkorn University Theses and Dissertations (Chula ETD)

In this study, a curcumin-loaded bacterial cellulose films were developed. Bacterial cellulose films were prepared by culturing Gluconacetobacter xylinus in coconut water-based medium. Curcumin, an active substance found in turmeric (Curcuma longa Linn), was then absorbed into never-dried bacterial cellulose pellicles by immersion in 0.5 and 1.0 mg/ml curcumin solutions, with absolute ethanol as solvent, for 24 hours. The curcumin-loaded bacterial cellulose pellicles were then air-dried. Controlled release of curcumin was achieved in buffer solutions containing Tween 80 and methanol additives, at pH 5.5 and 7.4. Curcumin-loaded bacterial cellulose films prepared with curcumin solutions at concentrations of 0.5 and 1.0 …

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Prevalence And Molacular Genetic Analysis Of Human Respiratory Syncytial Virus And Enterovirus 68 Among Children With Acute Lower Respiratory Tract Infection In Thailand, I-Lada Thongpan Jan 2017

Prevalence And Molacular Genetic Analysis Of Human Respiratory Syncytial Virus And Enterovirus 68 Among Children With Acute Lower Respiratory Tract Infection In Thailand, I-Lada Thongpan

Chulalongkorn University Theses and Dissertations (Chula ETD)

Respiratory syncytial virus (RSV) causes acute lower respiratory tract infection in infants and young children worldwide. A clear description of local RSV molecular epidemiology, evolution, and transmission requires detailed sequence data and can inform new strategies for virus control and vaccine development. To investigate the RSV burden in Thailand over six consecutive years (January 2012 to December 2017). There were two different methods to detect RSV in the present study. First, we screened 3306 samples obtained from children ≤5 years old with acute respiratory tract infection using semi-nested reverse-transcription polymerase chain reaction (RT-PCR) during January 2012 to December 2015. Second, …

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Isoquinoline Alkaloid Composition And Expression Of Biosynthetic Genes In Mechanically Wounded Sacred Lotus, Thitirat Meelaph Jan 2017

Isoquinoline Alkaloid Composition And Expression Of Biosynthetic Genes In Mechanically Wounded Sacred Lotus, Thitirat Meelaph

Chulalongkorn University Theses and Dissertations (Chula ETD)

Isoquinoline alkaloids, a plant secondary metabolites present in many plant species including lotus (Nelumbo nucifera Gaertn.). Lotus leaf contains high amount of bioactive nuciferine and N-nornuciferine. A set of enzyme including NCS, 6OMT and CNMT and WRKY transcription factors (WRKY TFs) has been reported to have corresponding function in the biosynthesis isoquinoline alkaloids. Thus, we performed quantitative analysis on expression level of these corresponing genes and on nuciferine and N-nornuciferine content in lotus leaf using mechanical wounding method. Sequence analysis clearly showed that all the targeted genes possess the conserved region belongs to their protein families. The accumulation of compound …

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Intracellular Trafficking Of Different Shaped Oxidized Carbon Nanoparticles, Banphot Jiangchareon Jan 2017

Intracellular Trafficking Of Different Shaped Oxidized Carbon Nanoparticles, Banphot Jiangchareon

Chulalongkorn University Theses and Dissertations (Chula ETD)

Oxidized carbon nanospheres (OCSs) and graphene oxide sheets (GOShs) were prepared by oxidation of graphite. Oxidized carbon nanotubes (OCTs) was synthesized from single-walled carbon nanotubes. All three shapes of oxidized carbon nanoparticles (OCNs) were used for investigation of their intracellular trafficking in human embryonic kidney cells (HEK293T), mouse macrophage cells (RAW264.7) and human liver cancer cells (HepG2). The results revealed all three shapes of OCNs were internalized into through HEK293T and RAW264.7 cells without endosomal trapping, including localization of all the three OCNs in HEK293T's lysosome. In case of RAW264.7 cells, OCTs and GOShs were localized in lysosomes, only OCSs …

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Development Of Molecular Technique For Risk Assessment And Risk Management Of Listeria Monocytogenes And L. Innocua In Frozen Cooked Chicken Plant, Chirapiphat Phraephaisarn Jan 2017

Development Of Molecular Technique For Risk Assessment And Risk Management Of Listeria Monocytogenes And L. Innocua In Frozen Cooked Chicken Plant, Chirapiphat Phraephaisarn

Chulalongkorn University Theses and Dissertations (Chula ETD)

Thailand is one of the largest cooked chicken meat exporting country in the world. Unfortunately, these products can be contaminated with L. monocytogenes causes the disease Listeriosis which carries a fatality rate of 30 to 40 percent. Therefore, the various importing countries including Japan and the European countries require a zero tolerance for L. monocytogenes in the cooked chicken products. Besides, all Listeria spp. occurrence, and not only L. monocytogenes, in these products is also unacceptable to the importers. Rejection of Listeria contaminated product can reach over 5 to 20 million baht per factory per year. Hence, the aim of …

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Effect Of Stapled Α-Helical Mastermind Like 1 (Sahm1) Peptide Conjugated With Gold Nanoparticles Toward Notch Signaling Pathway In Human Leukemia Jurkat Cell, Chonnicha Subkod Jan 2017

Effect Of Stapled Α-Helical Mastermind Like 1 (Sahm1) Peptide Conjugated With Gold Nanoparticles Toward Notch Signaling Pathway In Human Leukemia Jurkat Cell, Chonnicha Subkod

Chulalongkorn University Theses and Dissertations (Chula ETD)

Currently, cancer is the top cause of death in Thailand and caused by the growth and spreading of abnormal cells in an uncontrolled manner. For T-cell acute lymphoblastic leukemia (T-ALL), it was found that gene of encoding protein Notch is often mutated. In T-ALL, Notch signaling pathway is hyperactivated resulting in uncontrolled cell division. There are many types of cancer treatment such as surgery and chemotherapy but both approaches have serious side effects. Therefore, development of target drug delivery system is needed. In this study, the objective was to develop specific target drug delivery system by using gold nanoparticle (AuNP) …

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Antiproliferative Activity Against Jurkat Cells Of Sahm1 Peptide Conjugated Gold Nanoparticles, Mukthida Thippayamonta Jan 2017

Antiproliferative Activity Against Jurkat Cells Of Sahm1 Peptide Conjugated Gold Nanoparticles, Mukthida Thippayamonta

Chulalongkorn University Theses and Dissertations (Chula ETD)

In general, treatments of cancer may involve medication, surgery, radiation and chemotherapy. Although these treatments are successful at a certain satisfactory level, their side effects are not acceptable for some patients. Thus, many researches have been investigated alternative methods to cure cancer. A technique of interest is the use of targeted drug therapy in which drug is designed to selectively kill only the cancer cells and to do no harm on the normal cells. Stapled α-helical peptide derived from mastermind-like I or SAHM1 is a small peptide which has been reported to have antiproliferative activity against cancer cells. However, its …

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Separation And Purification Of Lactic Acid From Fermentation Broth By Reverse Osmosis, Natnirin Phanthumchinda Jan 2017

Separation And Purification Of Lactic Acid From Fermentation Broth By Reverse Osmosis, Natnirin Phanthumchinda

Chulalongkorn University Theses and Dissertations (Chula ETD)

Brackish water reverse osmosis (BWRO) and seawater reverse osmosis (SWRO) membranes were used in the 2-stage reverse osmosis (RO) unit to recover, pre-purify, and pre-concentrate lactic acid. Calcium lactate (CaLAC), sodium lactate (NaLAC), and ammonium lactate (NH4LAC) were used as model feed solutions. The operating pressure showed a pronounced effect on lactate passage through the first BWRO unit, and the Donnan exclusion effect and hydrogen bonding were responsible for cation rejection. Calcium ions were rejected at the BWRO unit because of low diffusion rate and charge interaction at the surface. However, monovalent ions formed hydrogen bonds with the carbonyl group …

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Bioactive Peptide Derived From Trypsin Hydrolysis Of Unicellular Cyanobacterium Synechococcus Sp., Rutairat Suttisuwan Jan 2017

Bioactive Peptide Derived From Trypsin Hydrolysis Of Unicellular Cyanobacterium Synechococcus Sp., Rutairat Suttisuwan

Chulalongkorn University Theses and Dissertations (Chula ETD)

A free radicals can promote denaturing reactions in many cellular components, which create oxidative stress and it results to damage in body tissue. The free radicals can causing many diseases including heart diseases, Alzheimer, inflammation and cancer. This research aimed to investigate the effect of bioactive peptides derived from Synechococcus sp. cultured for 21 days on antioxidant, anti-inflammation and anticancer activities. The Synechococcus sp. protein hydrolysate was prepared by trypsin digest and purified by ultrafiltration with molecular weight cut off membranes of 10, 5 and 3 kDa. The MW <3 kDa fraction showed the highest NO, ABTS and DPPH radical scavenging activities with IC50 values of 34.51±9.8 µg protein/mL, IC50 11.54±0.3 µg protein/mL and IC50 13.63±0.15 µg protein/mL, respectively. The F2 fraction from gel filtration chromatography showed the strongest NO and ABTS radical scavenging activities with IC50 values of 7.68±0.64 µg protein/mL and 9.74±0.2 µg protein/mL, respectively. The F2 was purified by RP-HPLC to yield four fractions. The 30 – 40 min sub-fraction (F2-4) was selected for further analysis by mass spectroscopy. Five isolated peptides with amino acid sequences of AILQSYSAGKTK; 1,265.69 Da, ALNKTHLIQTK; 1,265.74 Da, LLVHAPVK; 875.55 Da, IPDAHPVK; 875.48 Da and VVVLRDGAVQQLGTPR; 1,706.97 Da were identified. The F2-4 had higher DPPH and NO radical scavenging activity compared to the synthetic peptide. Moreover, AILQSYSAGKTK had the highest ABTS radical scavenging activity. Furthermore, the F2 fraction protected oxidation-induced DNA damage in pBR322, pKS and pUC19 cells. F2 fraction was selected to study anti-inflammatory and anticancer properties. Anti-inflammatory effect, F2 fraction showed no cytotoxicity toward RAW264.7 macrophage cells. RT-PCR and qRT-PCR results showed that F2 reduced gene expression of pro-inflammatory cytokines iNOS, TNF-α, COX-1, COX-2 and IL-6. For anticancer activity, F2 fraction showed high anticancer activities in the human colon cancer cells (SW620) according to cytotoxic activity (MTT assay), with IC50 values of 106.58±21.46 µg protein/mL. The F2 fraction activated the apoptotic pathway in SW620 cells after treatment for 24, 48 and 72 hours. The highest activities of caspases 3, 8 and 9 were observed after treatment for 72 hours. These findings suggested that unicellular cyanobacterium Synechococcus sp. may be used to develop for natural anti-inflammation and natural anticancer drugs.

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Specific Binding Of Hlas And Antigenic Peptides Associated With Behcet's Disease And Systemic Sclerosis, Sirilak Kongkaew Jan 2017

Specific Binding Of Hlas And Antigenic Peptides Associated With Behcet's Disease And Systemic Sclerosis, Sirilak Kongkaew

Chulalongkorn University Theses and Dissertations (Chula ETD)

Human leukocyte antigen (HLA) molecules are essential components for immune functions. There play a major role to present antigenic-peptide for T-cell receptor (TCR) recognition and immune activation. Association between HLA genes and infectious as well as autoimmune diseases are identified genetic risk factors. For instance, Behçet’s disease (BD) and systemic sclerosis (SSc) are autoimmunity result in chronic of multisystemic inflammation and visceral fibrosis, respectively. To investigate the link of HLAs correlated with self-peptides for BD and SSc, molecular dynamics (MD) simulations were applied on the HLAp complexes. For BD, a self-peptide named MICA-TM was modeled for two BD-association such as …

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Antibacterial Agents From Dracaena Cochinchinensis (Lour.) S.C.Chen And Eleutherine Americana (Aubl.) Merr. Ex K. Heyne, Ritbey Ruga Jan 2017

Antibacterial Agents From Dracaena Cochinchinensis (Lour.) S.C.Chen And Eleutherine Americana (Aubl.) Merr. Ex K. Heyne, Ritbey Ruga

Chulalongkorn University Theses and Dissertations (Chula ETD)

Fifteen compounds including two new anthraquinones (118-119), two naturally occurring new compounds (116-117) and eleven known compounds were successfully isolated from the CH2Cl2 extracts of Dracaena cochinchinensis and Eleutherine americana i.e. dihydrochalcones (5 and 23), pterostilbene (27), homoisoflavanones (15 and 82-84), naphthalenes (85 and 116), pyranonaphthoquinone (86) and anthraquinones (99-100 and 117-119). Among isolated compounds, (+)-eleutherin (86) was the most potent antibacterial agent against Propionibacterium acnes KCCM 41747, Staphylococcus aureus ATCC 25923, Streptococcus sobrinus KCCM 11898, Streptococcus mutans ATCC 25175 and Salmonella typhi ATCC 422 with inhibition zones of 14.7 to 18.0 mm and MIC values of 0.24 to 7.8 …

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Adaptation Of Terrilactibacillus Laevilacticus Sk5-6 For Growth And D-Lactic Acid Production In A Low Yeast Extract Concentration Medium, Srettapat Limsampancharoen Jan 2017

Adaptation Of Terrilactibacillus Laevilacticus Sk5-6 For Growth And D-Lactic Acid Production In A Low Yeast Extract Concentration Medium, Srettapat Limsampancharoen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Lactic acid has been extensively used in food, personal care, and pharmaceutical products. Besides, lactic acid can be used as the sole feedstock in the synthesis of polylactic acid (PLA), the biodegradable plastic. The demand of the bioplastics including PLA has been increased due to the environmental impact of petroleum based plastic; therefore, this expedited the market demand of lactic acid. It should be noted that the polymer grade lactic acid is unlike those commercially available products for food and pharmaceutical applications. The high purity of stereoisomer either D-lactate or L-lactate is mandatory in PLA polymerization. In addition, the production …

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Tyrosinase Inhibitors From Bark Of Talawrinta (Manilkara Kauki (L.) Dubard, Sirinada Srisupap Jan 2017

Tyrosinase Inhibitors From Bark Of Talawrinta (Manilkara Kauki (L.) Dubard, Sirinada Srisupap

Chulalongkorn University Theses and Dissertations (Chula ETD)

Melanin is essential for protecting human skin from ultraviolet radiation. However, its overproduction in the basal epithelial layer leads to hyperpigmentary disorders of the skin such as melasma, blemish and age spots. Tyrosinase is one of key enzymes in melanogenesis. Manilkara kauki (L.) Dubard or Talawrinta is one of the plants in genus Manilkara of Sapotaceae family. This study attempted to investigate in vitro total phenolic and flavonoid contents, antioxidant and tyrosinase inhibitory activities of crude extract of different parts of M. kauki and purify tyrosinase inhibitors from stem barks of M. kauki. Methanol and aqueous crude extracts of fruits, …

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Bioactive Compounds From Songkhla Province Mangrove-Derived Endophytic Fungi And From Heart-Leaved Moonseed Tinospora Baenzigeri Forman, Sujitra Hanthanong Jan 2017

Bioactive Compounds From Songkhla Province Mangrove-Derived Endophytic Fungi And From Heart-Leaved Moonseed Tinospora Baenzigeri Forman, Sujitra Hanthanong

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study has evaluated biological activities of isolated compounds from Tinospora baenzigeri stems collecting from two different areas, and from mangrove-derived endophytic fungi isolated from trees inhabiting in Songkhla province. Four new rearranged clerodane-type diterpenes (66-69), one new glycoside (70), and six known compounds (61, 71-75), were extracted from plant obtained from local market in Bangkok. Twelve compounds including a new rearranged clerodane-type diterpene (76), a new rearranged clerodane glycoside (77), five known compounds (44, 78-81), and five compounds counting 61, 66, 70 and 74-75 were obtained from plant collected in Bueng Kan Province. Structures of new compounds 66-69 and …

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Increasing Viability Of Probiotic Bacteria Co-Encapsulated With Prebiotic For Disease Resistance In White Shrimp Litopenaeus Vannamei, Suppharada Jantarathin Jan 2017

Increasing Viability Of Probiotic Bacteria Co-Encapsulated With Prebiotic For Disease Resistance In White Shrimp Litopenaeus Vannamei, Suppharada Jantarathin

Chulalongkorn University Theses and Dissertations (Chula ETD)

The main problem of probiotics is the low survival of these microorganisms in food products and sensitive to harsh conditions during food processing, storage and in the gastrointestinal tract. The suitable technology for protecting probiotics to maintain the high number of probiotic cells in product and survived within the host which is microencapsulation technique. The aim of this work was to investigate the effect of prebiotic which is inulin or Jerusalem artichoke on the survival of encapsulated Lactobacillus acidophilus TISTR 1338 within alginate matrix and double-coated with chitosan after freeze-drying process and heat processing at 70°C for 60 min. Furthermore, …

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Optimization Of Preparation Protein Hydrolysate By Protease G6 Using Response Surface Methodology And Free Radical Scavenging Activities From Feather Meal, Waleeporn Pibulpol Jan 2017

Optimization Of Preparation Protein Hydrolysate By Protease G6 Using Response Surface Methodology And Free Radical Scavenging Activities From Feather Meal, Waleeporn Pibulpol

Chulalongkorn University Theses and Dissertations (Chula ETD)

The aim of this research was to study the optimal condition for chicken feather meal protein hydrolysate production using a microbial protease, Protease G6. The experimentation was conducted using Central Composite Design (CCD) and Response Surface Methodology (RSM). The three independent variables including temperature (°C), time and enzyme-substrate ratio were studied. Optimization process for obtaining high yield of chicken feather meal protein hydrolysate was performed using response surface methodology (RSM) by optimizing a combination of three independent variables namely, temperature (41.59 – 58.41°C), time (0:38 - 7:21 h) and enzyme-substrate ratio (0.8 – 9.2) with degree of hydrolysis (DH) as …

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Tyrosinase Inhibitors From Sapodilla Plum Manilkara Zapota L., Sutthiduean Chunhakant Jan 2017

Tyrosinase Inhibitors From Sapodilla Plum Manilkara Zapota L., Sutthiduean Chunhakant

Chulalongkorn University Theses and Dissertations (Chula ETD)

Six different parts of Manilkara zapota which consisted of barks, flowers, fruits, leaves, roots and seeds were investigated for total phenolic content, total flavonoid content, antioxidant and antityrosinase activities. Methanol crude extract of flowers showed the highest total phenolic content (368.73 ± 0.65 mg GAE/g), while methanol crude extracts of seeds and roots showed high total flavonoid content (90.21 ± 0.57 and 89.03 ± 1.00 mg QE/g, respectively). Methanol crude extract of seeds showed the strongest DPPH (IC50 282.05 ± 0.60 μg/mL) and ABTS (IC50 205.11 ± 0.89 μg/mL) radical scavenging activities and showed the highest FRAP value of 296.46 …

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Diversity Of Mosses In Phu Kradueng National Park, Loei Province, Patsakorn Ajintaiyasil Jan 2017

Diversity Of Mosses In Phu Kradueng National Park, Loei Province, Patsakorn Ajintaiyasil

Chulalongkorn University Theses and Dissertations (Chula ETD)

The study of mosses diversity in Phu Kradueng National Park, Loei Province was explored from December 2014 to December 2016. The samples were collected along the 6 natural trails. A total of 501 samples were identified into 30 families, 55 genera, 100 species, 5 subspecies, and 9 varieties. Three families of mosses namely Sphagnaceae, Fissidentaceae, and Leucobryaceae were common families which included 11, 9 and 7 taxa, respectively. Additionally, the most common microhabitat was saxicolous with 45 species. The second and third common microhabitats were terrestrial and corticolous with 43 and 37 species, respectively. Additionally, there are 4 species which …

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Effects Of Carbon Fiber And Glass Fiber On Shape Memory Polymers Based On Benzoxazine-Epoxy Binary Systems, Jutamart Plylaharn Jan 2017

Effects Of Carbon Fiber And Glass Fiber On Shape Memory Polymers Based On Benzoxazine-Epoxy Binary Systems, Jutamart Plylaharn

Chulalongkorn University Theses and Dissertations (Chula ETD)

Recovery stress is an important parameter of shape memory polymers. Generally, recovery stress in SMPs can be enhanced by using fiber reinforced composites with suitable reinforcing agents such as carbon fiber or glass fiber. We aim to study effects of carbon fiber and glass fiber on thermal, mechanical, and shape memory properties of benzoxazine-epoxy SMPs, particularly on recovery stress enhancement. The compositions of carbon fiber and glass fiber reinforced benzoxazine-epoxy SMPs were in a range of 30 to 50vol%. The carbon fiber and glass fiber reinforced benzoxazine-epoxy shape memory polymer was compression-molded at 170oC under a pressure of 10 MPa …

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Photocatalytic Degradation Of Methylene Blue Over Polydopamine-Ag/Tio2, Kamonthip Tammarakwattana Jan 2017

Photocatalytic Degradation Of Methylene Blue Over Polydopamine-Ag/Tio2, Kamonthip Tammarakwattana

Chulalongkorn University Theses and Dissertations (Chula ETD)

This research investigated the effect of silver and polydopamine (PDA) doping on TiO2 catalysts for the photocatalytic degradation of methylene blue in aqueous phase. Titanium dioxide was synthesized via a sol-gel method and was calcined at 400 °C for two hours. Silver was loaded onto TiO2 by an incipient wetness impregnation method. The amount of Ag was varied from 0.5%wt to 3%wt. Then polydopamine-Ag/TiO2 catalyst was prepared by an impregnation method, using dopamine as a precursor. The amounts of polydopamine were 5%wt and 10%wt. All catalysts were characterized by N2 physisorption, ICP-OES, XRD, FTIR, CO chemisorption, Photoluminescence, and UV-Visible spectrophotometry. …

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Performance Comparison Of Different Membrane Micro-Channel Reactors For Methanol Production From Biogas And Hydrogen, Khunnawat Ountaksinkul Jan 2017

Performance Comparison Of Different Membrane Micro-Channel Reactors For Methanol Production From Biogas And Hydrogen, Khunnawat Ountaksinkul

Chulalongkorn University Theses and Dissertations (Chula ETD)

In order to integrate simultaneous methanol production with biogas upgrading in a single unit, the computational fluid dynamics (CFD) models of a triple pipe tubular membrane reactor (TMR) and a planar membrane micro-structured reactor (MMR) were established via COMSOL Multiphysics 5.3a and compared their effect on reactor performance under predetermined condition. For the conceptual design of these reactors, the channel inside the reactors can be divided into three parts; biogas channel (BC), reaction channel (RC) and sweep gas channel (SC). The CO2 in biogas entering into BC is separated through CO2 selective membrane to produce methanol inside RC. Afterwards, water …

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Preparation Of Stable Hydrothermal Carbon-Based Catalyst From Defatted Rice Bran And Glucose For Biomass Conversion To 5-Hydroxymethylfurfural, Piyaporn Wataniyakul Jan 2017

Preparation Of Stable Hydrothermal Carbon-Based Catalyst From Defatted Rice Bran And Glucose For Biomass Conversion To 5-Hydroxymethylfurfural, Piyaporn Wataniyakul

Chulalongkorn University Theses and Dissertations (Chula ETD)

This research investigated the preparation of hydrothermal carbon-based catalysts (HTC-SO3H) derived from defatted rice bran (DRB) and glucose for biomass conversion to 5-hydroxymethylfurfural (HMF). Firstly, the effects of hydrothermal carbonization conditions: temperature (180-250 °C) and time (1-8 h) on the yield and the chemical characteristics of the hydrothermal carbons (HTCs) were investigated. The HTCs were then sulfonated with sulfuric acid to obtain HTC-SO3H catalysts. The stability of the catalysts was evaluated based on the amount of biomass conversion products leached into the water at a specified biomass conversion condition. Since no HMF and furfural, and only small amounts of levulinic …

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