Digital Commons Network^™

Genotype, Environment, Seeding Rate, And Top-Dressed Nitrogen Effects On End-Use Quality Of Modern Nebraska Winter Wheat, Madhav Bhatta, Teshome Regassa, Devin J. Rose, P. Stephen Baenziger, Kent M. Eskridge, Dipak K. Santra, Rachana Poudel

Department of Agronomy and Horticulture: Faculty Publications

Background: Fine-tuning production inputs such as seeding rate, nitrogen (N), and genotype may improve end-use quality of hard red winter wheat (Triticum aestivium L.) when growing conditions are unpredictable. Studies were conducted at the Agronomy Research Farm (ARF; Lincoln, NE, USA) and the High Plains Agricultural Laboratory (HPAL; Sidney, NE, USA) in 2014 and 2015 in Nebraska, USA, to determine the effects of genotype (6), environment (4), seeding rate (3), and flag leaf top-dressed N (0 and 34 kg N ha⁻¹) on the end-use quality of winter wheat.

Results: End-use quality traits were influenced by environment, genotype, …

Author Correction: Genome-Guided Phylo-Transcriptomic Methods And The Nuclear Phylogenetic Tree Of The Paniceae Grasses, Jacob D. Washburn, James C. Schnable, Gavin C. Conant, Thomas P. Brutnell, Ying Shao, Yang Zhang, Martha Ludwig, Gerrit Davidse, J. Chris Pires

Department of Agronomy and Horticulture: Faculty Publications

The original version of this Article contained an error in the title of the paper, where the word “Phylogenetic” was incorrectly given as “Phylogentic”. This has now been corrected in the PDF and HTML versions of the Article, and in the accompanying Supplementary Information file.

Lettuce Learn: Student Reflections On Building And Sustaining A Community Donation Garden, Hannah Dankbar, Emily K. Zimmerman, Carrie Chennault, Andrea D. Basche, Jacqueline Ann Nester, Maritza Pierre, Gabrielle Roesch-Mcnally

Department of Agronomy and Horticulture: Faculty Publications

This article emerged from conversations that we and fellow graduate students have had in building a community donation garden. We created the garden with a vision of enacting food justice in our community, but over the past four years we have experienced complexities with our vision. In this article, we share the complexities with which we have wrestled, how we have encouraged thoughtful dialogue among fellow scholars about these shortcomings and the intricate workings of the agrifood system, and the lessons we have learned through these experiences as early-career scholar-activists. This article represents our collective and individual voices as graduate …

The Impact Of Continuous Living Cover On Soil Hydrologic Properties: A Meta-Analysis, Andrea D. Basche, Marcia Delonge

Department of Agronomy and Horticulture: Faculty Publications

Increased rainfall variability due to climate change threatens the efficacy of critical soil ecosystem services. One strategy to negate effects of too much or not enough rainfall is to improve soil water properties. Practices that offer “continuous living cover” can enhance soil water storage and other soil hydrologic properties relative to annual crop systems, but to what extent such benefits can accrue, under different conditions, remains under-quantified. To address these uncertainties, we conducted a meta-analysis that included 27 studies representing 93 paired observations measuring two soil hydrologic properties: porosity and the water retained at field capacity. All experiments compared the …

Research Topics To Scale Up Cover Crop Use: Reflections From Innovative Iowa Farmers, Andrea D. Basche, Gabrielle E. Roesch-Mcnally

Department of Agronomy and Horticulture: Faculty Publications

Cover crops as a conservation practice continue to receive attention from farmers, researchers, media, and policy makers, given their ability to effectively reduce water pollution and improve soil quality. Recent estimates of cover crop use across the midwestern Corn Belt, as well as the United States, demonstrate large acreage increases over the last number of years. The annual Sustainable Agriculture Research and Education–Conservation Technology Information Center (SARE– CTIC) survey found that nationally cover crop acreage doubled from 2011 to 2016, based on farmers self-reporting cover crop planting (CTIC 2016). However, the total cover crop acreage based on 2012 Census of …

Genotyping-By-Sequencing Derived High-Density Linkage Map And Its Application To Qtl Mapping Of Flag Leaf Traits In Bread Wheat, Waseem Hussain, P. Stephen Baenziger, Vikas Belamkar, Mary J. Guttieri, Jorge P. Venegas, Amanda Easterly, Ahmed Sallam, Jesse Poland

Department of Agronomy and Horticulture: Faculty Publications

Winter wheat parents ‘Harry’ (drought tolerant) and ‘Wesley’ (drought susceptible) were used to develop a recombinant inbred population with future goals of identifying genomic regions associated with drought tolerance. To precisely map genomic regions, high-density linkage maps are a prerequisite. In this study genotyping-by-sequencing (GBS) was used to construct the high-density linkage map. The map contained 3,641 markers distributed on 21 chromosomes and spanned 1,959 cM with an average distance of 1.8 cM between markers. The constructed linkage map revealed strong collinearity in marker order across 21 chromosomes with POPSEQ-v2.0, which was based on a high-density linkage map. The reliability …

Shifts In Microbial Communities In Soil, Rhizosphere And Roots Of Two Major Crop Systems Under Elevated Co2 And O3, Peng Wang, Ellen L. Marsh, Elizabeth A. Ainsworth, Andrew D. B. Leakey, Amy M. Sheflin, Daniel P. Schachtman

Department of Agronomy and Horticulture: Faculty Publications

Rising atmospheric concentrations of CO₂ and O₃ are key features of global environmental change. To investigate changes in the belowground bacterial community composition in response to elevated CO₂ and O₃ (eCO₂ and eO₃) the endosphere, rhizosphere and soil were sampled from soybeans under eCO2 and maize under eO₃. The maize rhizosphere and endosphere α-diversity was higher than soybean, which may be due to a high relative abundance of Rhizobiales. Only the rhizosphere microbiome composition of the soybeans changed in response to eCO₂, associated with an increased abundance of nitrogen …

Genomic-Enabled Prediction Accuracies Increased By Modeling Genotype × Environment Interaction In Durum Wheat, Sivakumar Sukumaran, Diego Jarquin, José Crossa, Matthew Reynolds

Department of Agronomy and Horticulture: Faculty Publications

Genomic prediction studies incorporating genotype × environment (G×E) interaction effects are limited in durum wheat. We tested the genomic-enabled prediction accuracy (PA) of Genomic Best Linear Unbiased Predictor (GBLUP) models—six non-G × E and three G × E models—on three basic cross-validation (CV) schemes— in predicting incomplete field trials (CV2), new lines (CV1), and lines in untested environments (CV0)— in a durum wheat panel grown under yield potential, drought stress, and heat stress conditions. For CV0, three scenarios were considered: (i) leave-one environment out (CV0-Env); (ii) leave one site out (CV0- Site); and (iii) leave 1 yr out (CV0-Year). The …

Evaluation Of Jules-Crop Performance Against Site Observations Of Irrigated Maize From Mead, Nebraska, Karina Williams, Jemma Gornall, Anna Harper, Andy Wiltshire, Debbie Hemming, Tristan Quaife, Tim Arkebauer, David Scoby

Department of Agronomy and Horticulture: Faculty Publications

The JULES-crop model (Osborne et al., 2015) is a parametrisation of crops within the Joint UK Land Environment Simulator (JULES), which aims to simulate both the impact of weather and climate on crop productivity and the impact of croplands on weather and climate. In this evaluation paper, observations of maize at three FLUXNET sites in Nebraska (US-Ne1, US-Ne2 and US-Ne3) are used to test model assumptions and make appropriate input parameter choices. JULES runs are performed for the irrigated sites (US-Ne1 and US-Ne2) both with the crop model switched off (prescribing leaf area index (LAI) and canopy height) and with …

Science In The Supply Chain: Collaboration Opportunities For Advancing Sustainable Agriculture In The United States, Allison M. Thomson, Stewart Ramsey, Ed Barnes, Bruno Basso, Marlen Eve, Sasha Gennet, Patricio Grassini, Brandon Kliethermes, Marty Matlock, Eileen Mcclellen, Ed Spevak, Clifford S. Snyder, Mark D. Tomer, Chris Van Kessel, Tristram West, Grant Wick

Department of Agronomy and Horticulture: Faculty Publications

Consumers and corporations are increasingly interested in understanding the sustainability of agricultural supply chains and reducing the environmental impacts of food, fiber, feed, and fuel production. This emerging need to quantify environmental impacts from agricultural production creates an opportunity for collaboration with the scientific community. Without such collaboration, sustainability efforts risk failure by adopting unrealistic goals or misguided approaches. This commentary explores the role of science in Field to Market, a nonprofit organization developing a sustainability program for US commodity crops, and highlights opportunities to address emerging science challenges. We evaluate changes over the past 35 years in key environmental …

Genetic Characterization Of The Soybean Nested Association Mapping Population, Qijian Song, Long Yan, Charles Quigley, Brandon D. Jordan, Edward Fickus, Steve Schroeder, Bao-Hua Song, Yong-Qiang Charles An, David Hyten, Randall L. Nelson, Katy Rainey, William D. Beavis, Jim Specht, Brian Diers, Perry Cregan

Department of Agronomy and Horticulture: Faculty Publications

A set of nested association mapping (NAM) families was developed by crossing 40 diverse soybean [Glycine max (L.) Merr.] genotypes to the common cultivar. The 41 parents were deeply sequenced for SNP discovery. Based on the polymorphism of the single-nucleotide polymorphisms (SNPs) and other selection criteria, a set of SNPs was selected to be included in the SoyNAM6K BeadChip for genotyping the parents and 5600 RILs from the 40 families. Analysis of the SNP profiles of the RILs showed a low average recombination rate. We constructed genetic linkage maps for each family and a composite linkage map based on …

Editorial: Genomic Approaches For Improvement Of Understudied Grasses, Keenan Amundsen, Gautam Sarath, Teresa Donze-Reiner

Department of Agronomy and Horticulture: Faculty Publications

Grasses are diverse, spanning native prairies to high-yielding grain cropping systems. They are valued for their beauty and useful for soil stabilization, pollution mitigation, biofuel production, nutritional value, and forage quality; grasses encompass the most important grain crops in the world. There are thousands of distinct grass species and many have promiscuous hybridization patterns, blurring species boundaries. Resources for advancing the science and knowledgebase of individual grass species or their unique characteristics varies, often proportional to their perceived value to society. For many grasses, limited genetic information hinders research progress. Presented in this research topic is a brief snapshot of …

Expression Of The Maize Dof1 Transcription Factor In Wheat And Sorghum, Pamela A. Pena, Truyen Quach, Shirley Sato, Zhengxiang Ge, Natalya Nersesian, Taity Changa, Ismail M. Dweikat, Madhavan Soundararajan, Tom E. Clemente

Department of Agronomy and Horticulture: Faculty Publications

Nitrogen is essential for plant growth and development. Improving the ability of plants to acquire and assimilate nitrogen more efficiently is a key agronomic parameter that will augment sustainability in agriculture. A transcription factor approach was pursued to address improvement of nitrogen use efficiency in two major commodity crops. To this end, the Zea mays Dof1 (ZmDof1) transcription factor was expressed in both wheat (Triticum aestivum) and sorghum (Sorghum bicolor) either constitutively, UBI4 promoter from sugarcane, or in a tissue specific fashion via the maize rbcS1 promoter. The primary transcription activation target of ZmDof1 …

High-Throughput Profiling And Analysis Of Plant Responses Over Time To Abiotic Stress, Kira M. Veley, Jeffrey C. Berry, Sarah J. Fentress, Daniel P. Schachtman, Ivan Baxter, Rebecca Bart

Department of Agronomy and Horticulture: Faculty Publications

Sorghum (Sorghum bicolor (L.) Moench) is a rapidly growing, high-biomass crop prized for abiotic stress tolerance. However, measuring genotype-by-environment (G x E) interactions remains a progress bottleneck. We subjected a panel of 30 genetically diverse sorghum genotypes to a spectrum of nitrogen deprivation and measured responses using high-throughput phenotyping technology followed by ionomic profiling. Responses were quantified using shape (16 measurable outputs), color (hue and intensity), and ionome (18 elements). We measured the speed at which specific genotypes respond to environmental conditions, in terms of both biomass and color changes, and identified individual genotypes that perform most favorably. With this …

Pumping Plant Performance, Derrel L. Martin, William Kranz, Suat Irmak, Daran Rudnick, Chuck Burr, Steve Melvin

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

Irrigation accounts for a large portion of the energy used in Nebraska agriculture. This paper describes a method to estimate the cost of pumping water and compares the amount of energy used by a properly designed and well-maintained pumping plant, represented by the Nebraska Pumping Plant Performance Criteria (NPPPC). The results can help determine the feasibility of repairing the pumping plant. Methods to compare energy sources are also presented. We recommend that you periodically arrange with a well drilling company to test the efficiency of your pump. Worksheets for pumping plant performance are included in the appendix.

Moving Integrated Weed Management From Low Level To A Truly Integrated And Highly Specific Weed Management System Using Advanced Technologies, S. L. Young, S. K. Pitla, F. K. Van Evert, J. K. Schueller, F. J. Pierce

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Integrated weed management (IWM) is one of the most commonly referred to approaches for sustainable and effective weed control in agriculture, yet it is not widely practiced, likely because current IWM systems fail to meet performance expectations of growers. The effectiveness and value of IWM systems should increase with increasing application specificity and true integration made possible with contemporary advances in technology, information systems and decision support. IWM systems can be classified based on their degree of application specificity and level of integration of tactics. In the application specificity pathway, a tactic is applied at a range of scales, from …

Runoff Water Quality Characteristics Following Swine Slurry Application Under Broadcast And Injected Conditions, Nicole R. Schuster, Shannon L. Bartelt-Hunt, Lisa M. Durso, John E. Gilley, Xu Li, David B. Marx, Amy M. Schmidt, Daniel D. Snow

Department of Agricultural and Biological Systems Engineering: Faculty Publications

This study was conducted to measure the effects of swine slurry application method, time following slurry application, and runoff rate on selected water quality characteristics. Slurry from a commercial swine operation was broadcast or injected on field plots at a rate required to meet annual nitrogen requirements for corn. Rainfall simulation tests were conducted at five varying periods following slurry application. During each study period, three simulated rainfall events, separated by 24 h intervals, were applied for 30 min duration at an intensity of approximately 70 mm h^-1. Following the third rainfall simulation event, inflow was applied at …

Multiple Drivers Of Seasonal Change In Pri: Implications For Photosynthesis 1. Leaf Level, Anatoly A. Gitelson, John A. Gamon, Alexei E. Solovchenko

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The goal of this study was to explore the relationships between the photochemical reflectance index (PRI) at the leaf level and pigment pools, focusing on the constitutive role of pigments in influencing PRI over seasonal or ontogenetic time frames. The purpose was to re-evaluate the role of PRI as an indicator of seasonally shifting pigment (chlorophyll, carotenoid and anthocyanin) contents, and hence photosynthetic activity, across a range of tree and crop species. We studied natural vegetation — three tree species (maple, chestnut and beech) and two managed irrigated and rain-fed crop species (maize and soybean), contrasting in photosynthetic pathway and …

Multiple Drivers Of Seasonal Change In Pri: Implications For Photosynthesis 2. Stand Level, Anatoly A. Gitelson, John A. Gamon, Alexei E. Solovchenko

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The goal of this study was to explore the relationships between stand-level photochemical reflectance index (PRI) and canopy structure/ pigment pools, as well as light use efficiency (LUE) of photosynthetically active vegetation focusing on seasonal or ontogenetic time frames. PRI was originally designed as a means of assessing the xanthophyll cycle and LUE over short (e.g. diurnal) time frames, and few studies have explored the drivers of PRI over longer, seasonal time frames, particularly in crops having different photosynthetic pathways or canopy structures. Consequently, our purpose was to understand and quantify the drivers of PRI responses over seasonal time scales …

Optical Probing Of Gastrocnemius In Patients With Peripheral Artery Disease Characterizes Myopathic Biochemical Alterations And Correlates With Stage Of Disease, Ryan A. Becker, Kim Cluff, Nithyanandhi Duraisamy, Hootan Mehraein, Hussam Farhoud, Tracie Collins, George P. Casale, Iraklis I. Pipinos, Jeyamkondan Subbiah

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Peripheral artery disease (PAD) is a condition caused by atherosclerotic blockages in the arteries supplying the lower limbs and is characterized by ischemia of the leg, progressive myopathy, and increased risk of limb loss. The affected leg muscles undergo significant changes of their biochemistry and metabolism including variations in the levels of many key proteins, lipids, and nucleotides. The mechanisms behind these changes are poorly understood. The objective of this study was to correlate the severity of the PAD disease stage and associated hemodynamic limitation (determined by the ankle brachial index, ABI) in the legs of the patients with alterations …

Soil Water Content Monitoring For Irrigation Management: A Geostatistical Analysis, J. Burdette Barker, Trenton E. Franz, Derek M. Heeren, Christopher M.U. Neale, Joe D. Luck

Department of Agricultural and Biological Systems Engineering: Faculty Publications

With the increasing attention to site-specific or variable rate irrigation management, it is helpful to reconsider the quantity and placement of soil water monitoring locations in this context. Volumetric soil water content (θ_v) was monitored using a neutron probe (NP) at 72 locations in a center pivot irrigated field in eastern Nebraska. Variance reduction and temporal stability analyses were performed on θ_v from shallow (∼top 46 cm) and full profile (∼122 cm) readings for four monitoring cycles in the 2015 growing season and 2016 preseason. Eleven additional cycles were included for a subset of the data for …

Fuel Consumption Models For Tractor Test Reports, Michael F. Kocher, Bryan J. Smith, Roger M. Hoy, Jeffrey C. Woldstad, Santosh Pitla

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Five models for estimating fuel consumption for agricultural tractors with partial drawbar loads were compared. Data were collected from eight John Deere tractors, JD 7230R (e23), 7250R (e23), 7270R (e23), 7290R (e23), 8320R (16 speed), 7290R (IVT), 8345RT (IVT), 8370R (IVT), on the drawbar test track at the Nebraska Tractor Test Lab. The tractors were tested with seven load levels per speed at three different travel speeds as close as possible to 7.5, 10, and 13 km h-1. The IVT tractors were operated in auto mode, and the geared tractors were shifted up three gears and throttled back to the …

A Comprehensive Dosimetric Study On Switching From A Type-B To A Type-C Dose Algorithm For Modern Lung Sbrt, Christina Zhou, Nathan Bennion, Rongtao Ma, Xiaoying Liang, Shuo Wang, Kristina Zvolanek, Megan Hyun, Xiaobo Li, Sumin Zhou, Weining Zhen, Chi Lin, Andrew Wahl, Dandan Zheng

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Background: Type-C dose algorithms provide more accurate dosimetry for lung SBRT treatment planning. However, because current dosimetric protocols were developed based on conventional algorithms, its applicability for the new generation algorithms needs to be determined. Previous studies on this issue used small sample sizes and reached discordant conclusions. Our study assessed dose calculation of a Type-C algorithm with current dosimetric protocols in a large patient cohort, in order to demonstrate the dosimetric impacts and necessary treatment planning steps of switching from a Type-B to a Type-C dose algorithm for lung SBRT planning.

Methods: Fifty-two lung SBRT patients were included, each …

Creation Of An Injectable In Situ Gelling Native Extracellular Matrix For Nucleus Pulposus Tissue Engineering, Rebecca A. Wachs, Ella N. Hoogenboezem, Hammad I. Huda, Shangjing Xin, Stacy L. Porvasnik, Christine E. Schmidt

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Background Context: Disc degeneration is the leading cause of low back pain and is often characterized by a loss of disc height, resulting from cleavage of chondroitin sulfate proteoglycans (CSPGs) present in the nucleus pulposus. Intact CSPGs are critical to water retention and maintenance of the nucleus osmotic pressure. Decellularization of healthy nucleus pulposus tissue has the potential to serve as an ideal matrix for tissue engineering of the disc because of the presence of native disc proteins and CSPGs. Injectable in situ gelling matrices are the most viable therapeutic option to prevent damage to the anulus fibrosus and future …

In Vitro Porcine Blastocyst Development In Three-Dimensional Alginate Hydrogels, Jeremy R. Miles, Taylor D. Laughlin, Catherine Sargus-Patino, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Appropriate embryonic and fetal development significantly impact pregnancy success and, therefore, the efficiency of swine production. The pre-implantation period of porcine pregnancy is characterized by several developmental hallmarks, which are initiated by the dramatic morphological change that occurs as pig blastocysts elongate from spherical to filamentous blastocysts. Deficiencies in blastocyst elongation contribute to approximately 20% of embryonic loss, and have a direct influence on within-litter birth weight variation. Although factors identified within the uterine environment may play a role in blastocyst elongation, little is known about the exact mechanisms by which porcine (or other species’) blastocysts initiate and progress through …

Tractor Power Take-Off Torque Measurement And Data Acquisition System, James Bw Roeber, Santosh Pitla, Roger M. Hoy, Joe D. Luck, Michael F. Kocher

Department of Agricultural and Biological Systems Engineering: Faculty Publications

With the mechanization of agricultural operations, agricultural machinery management has become an extensive research field. Sizing tractors and implements to provide the most efficient power transfer has become an ongoing process with advances in technology. Utilization of the rotational power transferred through gear trains from the tractor engine to the power take-off (PTO) shaft is one of the most efficient methods of power transfer to an implement. This research used commercially available torque sensors that were installed on a tractor PTO shaft for measuring the torque delivered to an implement. The torque sensor was calibrated using the Nebraska Tractor Test …

Laboratory And Full Boom-Based Investigation Of Nozzle Setup Error Effects On Flow, Pressure, And Spray Pattern Distribution, Shane H. Forney, Joe D. Luck, Michael F. Kocher, Santosh Pitla

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Pesticide application is an integral part of crop production, and ground-based agricultural boom sprayers are used extensively to apply pesticides to the crop canopy or soil surface across millions of acres in the United States. Efficient application is necessary to minimize costs and limit adverse environmental impacts. The goals of this study were to provide quantified measurements on the effects of nozzle setup errors on spray pattern uniformity and evaluate how laboratory patternator-based simulations would compare to measurements on a full spray boom. More specific objectives were to determine the effects of factors such as nozzle lateral angle, nozzle spacing, …

Tractor Power Take-Off Torque Measurement And Data Acquisition System, James B. W. Roeber, Santosh K. Pitla, R. M. Hoy, J. D. Luck, Michael F. Kocher

Department of Agricultural and Biological Systems Engineering: Faculty Publications

With the mechanization of agricultural operations, agricultural machinery management has become an extensive research field. Sizing tractors and implements to provide the most efficient power transfer has become an ongoing process with advances in technology. Utilization of the rotational power transferred through gear trains from the tractor engine to the power take-off (PTO) shaft is one of the most efficient methods of power transfer to an implement. This research used commercially available torque sensors that were installed on a tractor PTO shaft for measuring the torque delivered to an implement. The torque sensor was calibrated using the Nebraska Tractor Test …

Laboratory And Full Boom-Based Investigation Of Nozzle Setup Error Effects On Flow, Pressure, And Spray Pattern Distribution, S. H. Forney, J. D. Luck, Michael F. Kocher, Santosh K. Pitla

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Pesticide application is an integral part of crop production, and ground-based agricultural boom sprayers are used extensively to apply pesticides to the crop canopy or soil surface across millions of acres in the United States. Efficient application is necessary to minimize costs and limit adverse environmental impacts. The goals of this study were to provide quantified measurements on the effects of nozzle setup errors on spray pattern uniformity and evaluate how laboratory patternator-based simulations would compare to measurements on a full spray boom. More specific objectives were to determine the effects of factors such as nozzle lateral angle, nozzle spacing, …

Chitosan-Zein Nano-In-Microparticles Capable Of Mediating In Vivo Transgene Expression Following Oral Delivery, Eric Farris, Deborah M. Brown, Amanda Ramer-Tait, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The oral route is an attractive delivery route for the administration of DNA-based therapeutics, specifically for applications in gene therapy and DNA vaccination. However, oral DNA delivery is complicated by the harsh and variable conditions encountered throughout gastrointestinal (GI) transit, leading to degradation of the delivery vector and DNA cargo, and subsequent inefficient delivery to target cells. In this work, we demonstrate the development and optimization of a hybrid-dual particulate delivery system consisting of two natural biomaterials, zein (ZN) and chitosan (CS), to mediate oral DNA delivery. Chitosan-Zein Nano-in-Microparticles (CS-ZN-NIMs), consisting of core Chitosan/DNA nanoparticles (CS/DNA NPs) prepared by ionic …