Digital Commons Network^™

Deepsouth Will Rise Again—The Argument In Favor Of The Federal Circuit's Holding In Promega Corp. V. Life Technologies, Christopher Ainscough

Washington Journal of Law, Technology & Arts

Two recent holdings from the United States Court of Appeals for the Federal Circuit in Promega Corp. v. Life Technologies Corp. have come under fire from members of the patent community. In Promega, the Federal Circuit held that i) 35 U.S.C. § 271(f)(1) does not require a third party to "actively induce the combination" of a patented invention, and ii) that a single component can be a "substantial portion" of the components of patented invention. In this Article, I argue that the Federal Circuit decided these issues correctly in light of the policy considerations that went into Congress's enactment …

Differential Requirements For Myeloid Leukemia Ifn-Γ Conditioning Determine Graft-Versus-Leukemio Resistance And Sensitivity, Catherine Matte-Martone, Jinling Liu, Meng Zhou, Maria Chikina, Douglas R. Green, John T. Harty, Warren D. Shlomchik

Engineering Management and Systems Engineering Faculty Research & Creative Works

The graft-versus-leukemia (GVL) effect in allogeneic hematopoietic stem cell transplantation (alloSCT) is potent against chronic phase chronic myelogenous leukemia (CP-CML), but blast crisis CML (BC-CML) and acute myeloid leukemias (AML) are GVL resistant. To understand GVL resistance, we studied GVL against mouse models of CP-CML, BC-CML, and AML generated by the transduction of mouse BM with fusion cDNAs derived from human leukemias. Prior work has shown that CD4+ T cell-mediated GVL against CP-CML and BC-CML required intact leukemia MHCII; however, stem cells from both leukemias were MHCII negative. Here, we show that CP-CML, BC-CML, and AML stem cells upregulate MHCII …

Mc-Ppea As A New And More Potent Inhibitor Of Clp-Induced Sepsis And Pulmonary Inflammation Than Fk866., Peixin Huang, Mark W Lee, Keivan Sadrerafi, Daniel P. Heruth, Li Q. Zhang, Dev Maulik, Shui Qing Ye

Manuscripts, Articles, Book Chapters and Other Papers

Our previous study indicated that overexpression of nicotinamide phosphoribosyltransferase (NAMPT) aggravated acute lung injury, while knockdown of NAMPT expression attenuated ventilator-induced lung injury. Recently, we found that meta-carborane-butyl-3-(3-pyridinyl)-2E-propenamide (MC-PPEA, MC4), in which the benzoylpiperidine moiety of FK866 has been replaced by a carborane, displayed a 100-fold increase in NAMPT inhibition over FK866. Here, we determined the effects of MC4 and FK866 on cecal ligation and puncture (CLP) surgery-induced sepsis in C57BL/6J mice. MC4 showed stronger inhibitory effects than FK866 on CLP-induced mortality, serum tumor necrosis factor α (TNFα) levels, pulmonary myeloperoxidase activity, alveolar injury, and interleukin 6 and interleukin1β messenger …

Decellularized Wharton's Jelly From Human Umbilical Cord As A Novel 3d Scaffolding Material For Tissue Engineering Applications., Sushma Jadalannagari, Gabriel Converse, Eric Buse, Michael Filla, Maria T. Villar, Antonio Artigues, Adam J. Mellot, Jinxi Wang, Michael S. Detamore, Richard A. Hopkins, Omar S. Aljitawi, C Mcfall

Manuscripts, Articles, Book Chapters and Other Papers

In tissue engineering, an ideal scaffold attracts and supports cells thus providing them with the necessary mechanical support and architecture as they reconstruct new tissue in vitro and in vivo. This manuscript details a novel matrix derived from decellularized Wharton's jelly (WJ) obtained from human umbilical cord for use as a scaffold for tissue engineering application. This decellularized Wharton's jelly matrix (DWJM) contained 0.66 ± 0.12 μg/mg sulfated glycosaminoglycans (GAGs), and was abundant in hyaluronic acid, and completely devoid of cells. Mass spectroscopy revealed the presence of collagen types II, VI and XII, fibronectin-I, and lumican I. When seeded onto …

Genomics Of Primary Chemoresistance And Remission Induction Failure In Paediatric And Adult Acute Myeloid Leukaemia., Fiona C. Brown, Paolo Cifani, Esther Drill, Jie He, Eric Still, Shan Zhong, Sohail Balasubramanian, Dean Pavlick, Bahar Yilmazel, Kristina M. Knapp, Todd A. Alonzo, Soheil Meshinchi, Richard M. Stone, Steven M. Kornblau, Guido Marcucci, Alan S. Gamis, John C. Byrd, Mithat Gonen, Ross L. Levine, Alex Kentsis

Manuscripts, Articles, Book Chapters and Other Papers

Cure rates of children and adults with acute myeloid leukaemia (AML) remain unsatisfactory partly due to chemotherapy resistance. We investigated the genetic basis of AML in 107 primary cases by sequencing 670 genes mutated in haematological malignancies. SETBP1, ASXL1 and RELN mutations were significantly associated with primary chemoresistance. We identified genomic alterations not previously described in AML, together with distinct genes that were significantly overexpressed in therapy-resistant AML. Defined gene mutations were sufficient to explain primary induction failure in only a minority of cases. Thus, additional genetic or molecular mechanisms must cause primary chemoresistance in paediatric and adult AML.

Effects Of Human State Park Visitation Rates On Escape Behavior Of White-Tailed Deer, Nicholas M. Sutton, Edward J. Heske

Human–Wildlife Interactions

State parks are typically established to preserve natural or native habitats for wildlife while simultaneously providing recreational experiences for humans. However, because of their proximity to urban centers, the level of human visitation associated with state parks may be highly variable. Little information has been published regarding the effect of human visitation levels on wildlife escape behavior in state parks. We evaluated flight initiation distances (FIDs) and buff er distances (i.e., the difference between alert and flight distances) for white-tailed deer (Odocoileus virginianus; deer) from September 2013 to August 2014 at 3 state parks in east-central Illinois with different human …

A Systematic Review Of Context Bias In Invasion Biology, Robert Warren

Biology Faculty Datasets

The language that scientists use to frame biological invasions may reveal inherent bias - including how data are interpreted. A frequent critique of invasion biology is the use of value-laden language that may indicate context bias. Here we use a systematic study of language and interpretation in papers drawn from invasion biology to evaluate whether there is a link between the framing of papers and the interpretation of results. We also examine any trends in context bias in biological invasion research. We examined 651 peer-reviewed invasive species competition studies and implemented a rigorous systematic review to examine bias in the …

Nest-Mediated Seed Dispersal, Robert Warren

Biology Faculty Datasets

Many plant seeds travel on the wind and through animal ingestion or adhesion; however, an overlooked dispersal mode may lurk within those dispersal modes. Viable seeds may remain attached or embedded within materials birds gather for nest building. Our objective was to determine if birds inadvertently transport seeds when they forage for plant materials to build, insulate and line nests. We also hypothesized that nest-mediated dispersal might be particularly useful for plants that use mating systems with self-fertilized seeds embedded in their stems. We gathered bird nests in temperate forests and fields in eastern North America and germinated the plant …

Reassessing Survival, Movement, Resource Selection, And Sightability Of Pronghorn In Western South Dakota, Adam Kauth

Electronic Theses and Dissertations

Limited information exists on the survival, movements, resource selection, and densities of pronghorn (Antilocapra americana) inhabiting sagebrush-steppe regions within the Dakotas. Primary objectives of this study were to develop a sightability model for aerial surveying and to document survival rates and movement patterns for pronghorn in western South Dakota. Secondary objectives were to estimate seasonal home ranges, daily movements, determine cause-specific mortality, and evaluate summer and winter resource use and selection. Additionally, we evaluated exposure of pronghorn to novel diseases including Epizootic Hemorrhagic Disease (EHD), West Nile Virus (WNV), Blue Tongue Virus (BTV), Bovine Viral Diarrhea Virus (BVDV), Neospora, and …

Evaluation Of A Bioprocessed Soybean Meal On Nursery Pig Immune Status And Disease Resistance, Jacob R. Koepke

Electronic Theses and Dissertations

Weaning is a stressful time in a piglet’s life that can have negative impacts on performance and health if not managed properly. The objective of this research was to evaluate the effects of a bioprocessed SBM alone or in combination with spray-dried plasma (SDP) or fishmeal (FM) on immune response at weaning with no imminent disease threat and during a porcine reproductive and respiratory virus (PRRSV) challenge. In the first trial, 239 pigs (initial BW 6.56 ± 0.87 kg, 21 d of age) were used in a 35-d study and allotted to one of 4 dietary treatments (10 pens/trt) according …

Identification Of Cdks As Novel Targets Of Aspirin And Its Metabolites: A Potential Role In Cancer Prevention, Rakesh Dachineni

Electronic Theses and Dissertations

Background:
The pursuit of drugs that inhibit cyclin-dependent kinases (CDKs) has been an intense area of research for more than 15 years. Till date, although multiple CDK inhibitors have been identified and few are undergoing clinical trials, only two synthetic drugs have been approved by Food and Drug Administration (FDA) for use in the treatment of cancer. These two drugs are mainly used for the treatment of metastatic breast cancer in combination with other drugs; however they have toxicity associated with their use and extends patients life not more than 24 months. Therefore, there is an urgent need for …

Self-Assembled Architecture Of Natural Organic Matter, Cynthia Johnson-Edler

Electronic Theses and Dissertations

This investigation focuses on the determination of the architecture of the natural organic matter (NOM) contained within a soft coal-like material, an agricultural soil and a peat material. NOM has been extracted from bulk materials, fractionated, reassembled, then characterized by ¹³C Solid State NMR, Pulsed-Field Gradient NMR (PFG-NMR), and Small Angle Neutron Scattering (SANS). Interpretation of the data obtained by SANS has established that the majority of the components of NOM are mass fractals in solution and surface fractals in the solid state. Surface-to-volume ratios calculated with PFGNMR data indicate the NOM components studied have varying disk-like shapes. These …

Food Protein-Based Core-Shell Nanocarriers For Oral Drug Delivery Applications: (Influence Of Shell Composition On In Vitro And In Vivo Functional Performance Of Zein Nanocarriers, Md Saiful Islam

Electronic Theses and Dissertations

Oral delivery is the most preferred route for drug administration. Oral drug delivery is limited by poor physicochemical properties of drugs and physiological barriers in the gastrointestinal tract. To this end, there is a need for developing new carrier systems to enhance the oral bioavailability of poorly absorbed molecules. Food-grade biopolymers are attractive materials for developing drug delivery carriers’ due to their unique properties and proven safety. Six different core-shell nanocarriers were prepared using food-grade biopolymers including zein-casein (ZC) nanoparticles, zein-lactoferrin (ZLF) nanoparticles, zein-β-lactoglobulin (ZLG) nanoparticles, zein-whey protein isolate (ZWP) nanoparticles, zein-pluronic-lecithin (ZPL) nanoparticles and zein-PEG (ZPEG) micelles. The study …

Swift Foxes In Southwestern South Dakota: Assessing The Current Status Of A Reintroduced Population, Sarah Ann Nevison

Electronic Theses and Dissertations

Swift foxes (Vulpes velox) were reintroduced into Badlands National Park between 2003 and 2006 after being nearly extirpated from South Dakota in the early 1900’s. Genetic analysis provided strong evidence that the reintroduction was successful, but viability analysis indicated the population may be in jeopardy with a high probability of extinction. Recently, the population has declined due to various biotic and abiotic factors (e.g., recent weather patterns, effects of plague [Yersinia pestis], and increased coyote [Canis latrans] numbers). No information on the status of swift foxes has been collected since 2009. Between 2014 and 2016, the objectives of this study …

Growth Potential And Genetic Diversity Of Yellow Perch In South Dakota, Alex J. Rosburg

Electronic Theses and Dissertations

Yellow Perch Perca flavescens represent a valued sport fish throughout their range and are an important prey species for piscivorous fishes. In South Dakota, two distinct population types of Yellow Perch have been characterized that differ in growth, survival, and recruitment patterns. Fast growth populations exhibit high growth rates, high mortality, low population density, and inconsistent recruitment. In contrast, slow growth populations are characterized by reduced growth rates, low mortality, high population density, and relatively consistent recruitment. The role of genetics in contributing to these population characteristics is currently unknown. To address these questions, I used high-throughput restriction-site associated DNA …

Genetic Interference And Receptor Biology Of Neglected Influenza Viruses, Runxia Liu

Electronic Theses and Dissertations

Influenza B virus (IBV) is an important pathogen that infects humans and causes seasonal influenza epidemics. By using next-generation sequencing (NGS) approach, we analyzed total mRNAs extracted from A549 cells infected with B/Brisbane/60/2008, and identified four defective genomes in IBV with two from the polymerase basic subunit 1 (PB1) segment and the other two from the matrix (M) segment. Each of them can potently inhibit the replication of IBV. One derived from PB1 segment was able to interfere modestly with influenza A virus (IAV) replication. The productions of the four defective RNAs are not dependent on the cell types. The …

Fluency And Instruction In The Elementary Grades, Stacey Bakken

Graduate Research Papers

Elementary teachers are being challenged to increase student's oral reading fluency. This can be attributed to today's high demands and focus on students fluency scores from required assessments, focusing mostly on correct words per minute. This project examines the history of reading instruction along with the five essential components highlighted by the National Reading Panel (2000), specifically, fluency. This project defines reading fluency and assessments used, as well as describing best practices in supporting and developing reading fluency in the elementary classroom.

Impact Of Family Engagement On Child Outcomes In Preschool, Kathryn S. Ingham

Graduate Research Papers

This review considered the impact of family engagement on child outcomes in preschool and what variables impact the success of family engagement in preschool. Many child outcomes are affected by family engagement. This particular work focuses on child outcomes related to literacy and approaches to learning. The variables of the family engagement examined include demographics, culture, family unit, and socioeconomic status. Many forms and degrees of family engagement are described with the degree of success for family engagement determined by the actual participants. Findings of the review point towards family engagement promoting positive outcomes for children enrolled in preschool despite …

The Precautionary Principle: A Cautionary Note, Robert C. Jones

Animal Sentience

The precautionary principle regarding animal sentience is often used in decision-making about human actions that may cause harm to nonhuman animals. Birch (2017) develops an account of the precautionary principle requiring two pragmatic rules for its implementation. I support Birch's proposal but offer a cautionary note about relying on precautionary principles if one's ultimate goal is to emancipate animals from human domination.

Biosignature Preservation And Detection In Mars Analog Environments, L E. Hays, H V. Graham, D J. Des Marais, E M. Hausrath, B Horgan, T M. Mccollom, M N. Parenteau, S L. Potter Mcintyre, A J. Williams, K L. Lynch

Geoscience Faculty Research

This review of material relevant to the Conference on Biosignature Preservation and Detection in Mars Analog Environments summarizes the meeting materials and discussions and is further expanded upon by detailed references to the published literature. From this diverse source material, there is a detailed discussion on the habitability and biosignature preservation potential of five primary analog environments: hydrothermal spring systems, subaqueous environments, subaerial environments, subsurface environments, and iron-rich systems. Within the context of exploring past habitable environments on Mars, challenges common to all of these key environments are laid out, followed by a focused discussion for each environment regarding challenges …

Are Antibiotics, Other Than Roxithromycin, An Effective Way To Manage Joint Pain In Rheumatoid Arthritis?, Kelly A. Hoffman

PCOM Physician Assistant Studies Student Scholarship

OBJECTIVE: The objective of this selective EBM review is to determine “Are antibiotics, other than roxithromycin, an effective way to manage joint pain in rheumatoid arthritis?”

STUDY DESIGN: Review of three double-blind, randomized-controlled studies published in English between 2001-2007.

DATA SOURCES: Three randomized controlled trials published between 2001 and 2007 were found using PubMed/MEDLINE and Cochrane databases.

OUTCOMES MEASURED: Clinical outcome for all three studies used American College of Rheumatology (ACR) criteria, which assesses joints for swelling and tenderness.

RESULTS: In the Odell et al.1 study, at 24 months 28 participants did meet the ACR50 …

Is 100mg Flibanserin Effective In Increasing The Number Of Sexually Satisfying Events (Sse) In Women With Hypoactive Sexual Desire Disorder (Hsdd)?, Bridget M. Frymoyer

PCOM Physician Assistant Studies Student Scholarship

OBJECTIVE: The objective of this selective EBM review is to determine whether or not 100mg Flibanserin is effective in increasing the number of sexually satisfying events in women with HSDD.

STUDY DESIGN: Review of three, placebo-controlled trials in which women > 18 years old with HSDD were treated with either 100mg Flibanserin or a Placebo once daily qhs for 24 weeks

DATA SOURCES: Three randomized, placebo-controlled trials found via PubMed searches and published in English peer-reviewed journals between 2011-2014

OUTCOMES MEASURED: Outcomes measured were the number of SSE over 4 weeks and analyzed with Wilcoxon’s rank-sum test. …

Is Methotrexate (Mtx) An Effective Treatment In Partially Or Totally Reducing The Symptoms Of Myasthenia Gravis (Mg) Patients?, Brandi S. Flyte

PCOM Physician Assistant Studies Student Scholarship

OBJECTIVE: The objective of this selective EBM review is to determine whether or not Methotrexate (MTX) is an effective treatment in partially or totally reducing the symptoms of Myasthenia Gravis (MG) patients.

STUDY DESIGN: Review of three English language studies; one Case Series Study following 3 patients with concomitant MG and Rheumatoid Arthritis treated with MTX published in 2014, one Single-Blind Randomized Control Trial (RCT) comparing MTX and AZA (Azathioprine) published in 2011, and one Double-blind, placebo-controlled Randomized Control Trial published in 2016.

DATA SOURCES: Three primary research studies published in peer reviewed journals; found on the …

Is Mindfulness-Based Therapy Effective In Increasing Total Sleep Time In Adults?, Johanna F. Detwiler

PCOM Physician Assistant Studies Student Scholarship

Objective: The objective of this selective evidence based medicine review is to determine whether or not mindfulness-based therapy is effective in increasing total sleep time in adults.

Study design: Review of three randomized controlled trials published in 2011, 2014 and 2015, all English language.

Data source-The three randomized controlled trials that were used in this review were found using PubMed.

Outcomes measured: Total sleep time, sleep onset latency, wake after sleep onset, number of awakenings, time in bed, subjective sleep quality, sleep duration, habitual sleep efficiency, sleep disturbances, use of sleep medications, and daytime dysfunction were …

Is Oral Vitamin D Supplementation Safe And Effective For Adults As Therapy To Improve Symptoms Associated With Seasonal Affective Disorder (Sad) During Wintertime?, James Berking

PCOM Physician Assistant Studies Student Scholarship

Objective: The objective of this selective EBM review is to determine whether or not oral vitamin D supplementation is safe and effective for adults as therapy to improve symptoms associated with seasonal affective disorder (SAD) during wintertime.

Study Design: This review is based on three randomized controlled trials (RCTs) which were published in 2006, 2012, 2014. Two of the studies were double blind and placebo controlled. The studies evaluated the efficacy of oral vitamin D supplementation for improvement of seasonal affective disorder (SAD) symptoms. One of the studies also evaluated safety.

Data Sources: All articles used were …

Does Adalimumab Effectively Treat Moderate To Severe Chronic Plaque Psoriasis In Adults?, Anthony J. Audia

PCOM Physician Assistant Studies Student Scholarship

OBJECTIVE: The objective of this EBM review is to determine whether or not “Does adalimumab effectively treat moderate to severe chronic plaque psoriasis in adults?”

STUDY DESIGN: This review is based on two randomized controlled trials and one retrospective study. The studies compared the efficacy of adalimumab in reducing the body surface area affected by plaque psoriasis.

DATA SOURCES: All articles used in this review are peer reviewed journals, published in English, and found using PubMed and Ebscohost.

OUTCOMES MEASURED: For all studies, the effectiveness of adalimumab on moderate to severe plaque psoriasis was evaluated using …

Are Cams Safe And Effective In Improving Symptoms Of Adult Patients Over The Age Of 18 With Active Crohn Disease?, Amy Pellerito

PCOM Physician Assistant Studies Student Scholarship

Objective: The objective of this selective EBM review is to determine whether or not complimentary and alternative medicines are safe and effective in improving symptoms of adult patients over the age of 18 with active Crohn disease.

Study design: A review of three published, randomized controlled trials (RCTs), one published in 2010, and the second and third in 2014. All English language.

Data source: All 3 RCT’s were found using PubMed.

Outcomes measured: All three studies measured improvement of Crohn disease symptoms through the Crohn Disease Activity Index (CDAI), Hamilton’s Depression Scale (HAMD), the Inflammatory Bowel …

Is The Use Of The Chinese Herbal Medicine, Ningdong Granule, A Safe And Effective Alternative To Haloperidol For The Treatment Of Tic Symptoms In Pediatric Patients With Tourette Syndrome (Ts)?, Vincent Russell

PCOM Physician Assistant Studies Student Scholarship

OBJECTIVE The objective of this selective EBM review is to determine whether the Chinese herbal supplement, Ningdong granule, is a safe and effective alternative to haloperidol for the treatment of tic symptoms in pediatric patients with Tourette Syndrome.

STUDY DESIGN Systematic review of three randomized controlled trials published in 2009, 2010, and 2012.

DATA SOURCES Three randomized controlled trials were obtained using PubMed.

OUTCOMES MEASURED Efficacy of Ningdong granule (NDG) as compared to control groups based on decrease in tic severity and frequency as reported by Yale Global Tic Severity Scale as well as safety, as evidenced by prevalence of …

Does Lactobacillus Reuteri Dsm 17938 Reduce Daily Crying Times In Infants With Colic?, Taylor Kolle

PCOM Physician Assistant Studies Student Scholarship

Objective: The objective of this selective EBM review is to determine whether or not “Does Lactobacillus reuteri DSM 17938 reduce daily crying times in infants with colic?”

Study Design: Review of all English language primary studies published in 2010, 2013, 2015.

Data Sources: Three randomized, double blind, placebo controlled clinical trials found using PubMed.

Outcomes Measured: Daily average crying times were measured by parents who documented these in a journal.

Results: In all three randomized control studies by Chaeu et al. (2015), Savino et al, (2010), and Szajewska et al. (2013), they found that infants …

Silicon Carbide Based Surface Plasmon Resonance Waveguide Sensor With A Bimetallic Layer For Improved Sensitivity, Wei Du, Feng Zhao

Electrical and Computer Engineering Faculty Research & Creative Works

In this letter, silicon carbide (SiC) waveguide-based surface plasmon resonance optical sensor with an Ag-Au bilayer metal configuration was studied. The wide bandgap energy of SiC allows the sensor to operate in visible and near-infrared range. The spectrum of resonant wavelength with refractive index shows improved sensitivity and sensing resolution in the Au-Ag bilayer configuration than in the single Au layer configuration. A sensitivity of 2581 nm/RIU (refractive index unit) indicated by the shift of resonant wavelength with refractive index was observed from a refractive index range of n=1.34–1.36, showing that the sensor is desirable for sensing in a series …