Digital Commons Network^™

Isolation And Characterization Of A Shewanella Phage–Host System From The Gut Of The Tunicate, Ciona Intestinalis, Brittany Leigh, Charlotte Karrer, John P. Cannon, Mya Breitbart, Larry J. Dishaw

Marine Science Faculty Publications

Outnumbering all other biological entities on earth, bacteriophages (phages) play critical roles in structuring microbial communities through bacterial infection and subsequent lysis, as well as through horizontal gene transfer. While numerous studies have examined the effects of phages on free-living bacterial cells, much less is known regarding the role of phage infection in host-associated biofilms, which help to stabilize adherent microbial communities. Here we report the cultivation and characterization of a novel strain of Shewanella fidelis from the gut of the marine tunicate Ciona intestinalis, inducible prophages from the S. fidelis genome, and a strain-specific lytic phage recovered from surrounding …

Short Organic Carbon Turnover Time And Narrow 14C Age Spectra In Early Holocene Wetland Paleosols, Lael Vetter, Brad E. Rosenheim, Alvaro Fernandez, Torbjörn E. Törnqvist

Marine Science Faculty Publications

Paleosols contain information about the rates of soil organic carbon turnover when the soil was actively forming. However, this temporal information is often difficult to interpret without tight stratigraphic control on the age of the paleosol. Here we apply ramped pyrolysis/oxidation (Ramped PyrOx) ¹⁴C analyses to evaluate age spectra of transgressive early Holocene paleosols from the Mississippi Delta in southeastern Louisiana, USA. We find ¹⁴C age spectra from soil organic matter (SOM) in both paleosols and overlying basal peats that represent variability in age that is close to, or only slightly greater than, analytical uncertainty of ¹⁴C …

Ice Formation And The Risk Of Chloride Toxicity In Shallow Wetlands And Lakes, Hilary A. Dugan, Greta Helmueller, John J. Magnuson

Marine Science Faculty Publications

The process of ice formation in shallow waterbodies can increase chloride to toxic levels in waterbodies already impacted by chloride loading from road salt (NaCl deicer) application. Chloride concentrations were measured bi-weekly in a shallow, urban wetland in Madison, Wisconsin. We found that in this shallow waterbody, ice thickening doubled chloride concentrations from ion exclusion as the water froze. To understand the role of ice formation and ion exclusion, we constructed a numerical model to predict chloride concentrations beneath the ice resulting from ion exclusion. Where chloride levels already are elevated above background and flushing rates are low, ice thickening …

Predicting Sargassum Blooms In The Caribbean Sea From Modis Observations, Mengqiu Wang, Chuanmin Hu

Marine Science Faculty Publications

Recurrent and significant Sargassum beaching events in the Caribbean Sea (CS) have caused serious environmental and economic problems, calling for a long-term prediction capacity of Sargassum blooms. Here we present predictions based on a hindcast of 2000–2016 observations from Moderate Resolution Imaging Spectroradiometer (MODIS), which showed Sargassum abundance in the CS and the Central West Atlantic (CWA), as well as connectivity between the two regions with time lags. This information was used to derive bloom and nonbloom probability matrices for each 1° square in the CS for the months of May–August, predicted from bloom conditions in a hotspot region in …

Modulating The Innate Immune Response To Electrospun Scaffolds And Polymer Degradative Byproducts, Daniel Abebayehu

Theses and Dissertations

Implanted biomaterials often induce inflammation that frequently leads to the foreign body response, fibrosis, and the failure of the implant. Thus, it is important to evaluate how cells interact with materials to promote a more regenerative response. It is critical to determine how to modulate the response of tissue resident innate immune cells, as they are among the first cells to interact with implanted materials. Among tissue resident innate immune cells are mast cells, which are inflammatory sentinels that degranulate and orchestrate the fate of other cell populations, such as monocytes/macrophages and lymphocytes. Mast cells have also been reported to …

Orthodontists' And Parents' Perspective Of Occlusion In Varying Anterior-Posterior Positions: A Comparative Study, David H. Lindsey

Theses and Dissertations

Objective: The purpose was to compare orthodontists’ and parents’ perception of orthodontic treatment outcomes in the anterior-posterior (AP) dimension. Assessment of treatment time and compliance were also investigated.

Material and Methods: Parallel surveys for orthodontists (n=1000) and parents (n=750) displayed occlusions from 3 mm Class III (Cl III:3) to 3 mm Class II. Participants rated occlusal relationships on a 100 mm VAS from least to most acceptable (0-100).

Results: 233 orthodontists (23%) and 243 parents (32%) responded. Orthodontists (mean=93.9, 25.9) and parents (mean=80.7, 40.9) rated Class I (Cl I) occlusion most and Cl III:3 least acceptable. No significant difference was …

Abuse-Related Behavioral Effects Of Oxycodone In The Mouse And Their Modulation By Hiv-1 Tat Expression, Rachel M. Enga

Theses and Dissertations

Abuse of prescription opioids has become epidemic and oxycodone is among the most frequently abused of these drugs. Opioid misuse is a risk factor for HIV infection and its chronic use by HIV-infected individuals can be accompanied by worsened progression to AIDS, cellular damage, and behavioral deficits collectively termed “neuroAIDS”. This toxicity is likely attributable, in part, to the interaction of opioids with the neurotoxic HIV-1 Tat protein. The ultimate objective of this dissertation was to characterize the interaction of HIV-1 Tat expression with the abuse-related effects of oxycodone. Physical dependence, drug self-administration, and sensitization are three classes of phenomena …

Thyroid Hormone As A Method Of Reducing Damage To Donor Hearts After Circulatory Arrest, William P. Adams

Theses and Dissertations

There is a chronic lack of donor hearts to meet the need for heart transplant both in the US and worldwide. Further, the use of available hearts is limited by the short period between collection and implantation during which the heart can be safely preserved ex vivo. Using mid-thermic Langendorff machine perfusion, we have been able to preserve the metabolic function of a healthy heart for up to 8 hours, twice the limit for current static cold storage. We have also been able to preserve the metabolic function of a damaged DCD Heart collected 30 minutes after cardiac arrest …

Design For Disassembly - A Circular Approach, Wajiha Pervez

Theses and Dissertations

As the world becomes increasingly aware of the need to better care for the environment, innovative business models are helping to counter the damage of the fast fashion system - a phenomenon in the fashion industry whereby production processes are expedited in order to get new trends to the market as quickly and cheaply as possible.

Designing products with a focus on their renewability can shift the product-consumer relationship. The closed loop concept of a “circular economy” is emerging as a viable and promising solution to the current linear business model.

This study explores the possibilities of a more mindful …

Uncovering The Progress Of Planning For Vulnerability To Sea-Level Rise & Coastal Storms: A Plan Evaluation Of Norfolk, Va & New York City, Eric K. Borchers

Theses and Dissertations

In response to recent storms like Superstorm Sandy and sea-level rise influenced by climate change, cities, particularly those located at the coast, have taken initiative to combat these growing threats with adaptive urban planning. Although civilians residing in susceptible neighborhoods are often the most vulnerable socioeconomically, there has been minimal evidence that planning has accounted for the characteristics of vulnerability. This thesis evaluates the recent planning efforts and vulnerability of Norfolk, VA and New York City to gauge the progress being made toward reducing citizen vulnerability and raising adaptability and preparedness. The most recent peer-reviewed research is consulted to forge …

Optimization Of In Vitro Transcription/Translation Conditions For In Vitro Compartmentalization Studies And Synthesis Of 4-Fluorohistidine, Christine Ring

Theses and Dissertations

Genetic code expansion allows the incorporation of non-canonical amino acids with a variety of new functional groups: fluorescent amino acids,^1-3 azides,^4-6 alkynes,^5-10 and photocrosslinkers.^4,11,12 This incorporation requires the evolution of new tRNA/aminoacyl tRNA sythetase pairs. Traditionally screenings of novel tRNA/aminoacyl tRNA synthetase pairs have been done in vivo. While these in vivo screenings have proven robust, they are limited in multiple ways: non-canonical amino acids (ncAAs) must be nontoxic and bioavailable. Furthermore, library size is limited by transformation efficiency. Lastly, in vivo screenings require substantial amounts of the target ncAA, which is often not available …

Stability And Bioactivities Of Mesona Chiensis Benth Extract Microbeads Under Simulated Gastrointestinal Digestion, Chonnipa Wongverawattanakul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Alginate-calcium microencapsulation of Mesona chinensis extract (MCE) was conducted in this study. Firstly, the MCE was encapsulated by using calcium chloride (3 and 5% w/v) and sodium alginate (1.5 and 1.8% w/v) to form MC microbeads (MCB). The MCB with condition of 1.5% w/v sodium alginate, 3% w/v calcium chloride and 75% w/v MCE demonstrated the highest %encapsulation efficiency and suitable spherical shape among other conditions. According to Fourier-transform infrared spectroscopy (FT-IR), there was no strong chemical interaction between alginate and MCE. After simulated digestion, the total phenolic content (TPC) and antioxidant activity measured by ferric reducing antioxidant power (FRAP) …

Development Of Novel Electrocoagulation Reactor (Ecr) For Turbidity Removal And Decolorization From Textile Industry Wastewater, Penghour Hong

Chulalongkorn University Theses and Dissertations (Chula ETD)

High concentration of dyes together with turbidity were frequently found in the effluents of textile industry wastewater, which are the toxic substances. To remove these contaminants, the combination between electrocoagulation process (EC) and separation has been proposed in this study. The objective of this present work is to design and evaluate the new electrocoagulation reactor (ECR) for treating dye and turbidity from synthetic textile wastewater. The optimization of electrode configuration and design parameter were examined with the batch column reactor for containing 4 liters of wastewater. The result showed that monopolar arrangement within the inner gap 1.5 cm and current …

Effect Of Operating Conditions And Ion Contaminations On Electro Coagulation And Oxidation Processes For Water Quality Improvement, Vouchlay Theng

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objective of this study is to investigate the effect of ion contaminations and operating conditions on Electro Coagulation (EC) and Oxidation (EO) in turbidity and natural organic matter (NOM) removal. The experiments were conducted in 4 liters column. Ferrous and calcium were chosen as contaminated ions. The operating condition was varied in terms of initial pH and current density. 100 NTU turbidity and 70 mg/L NOM were synthesized from bentonite and humic acid (HA), respectively. The results showed that bipolar arrangement of electrodes with 2 cm gap was the optimal condition in terms of gas flow rate and electrode …

Narrative Economics, Robert J. Shiller

Cowles Foundation Discussion Papers

This address considers the epidemiology of narratives relevant to economic fluctuations. The human brain has always been highly tuned towards narratives, whether factual or not, to justify ongoing actions, even such basic actions as spending and investing. Stories motivate and connect activities to deeply felt values and needs. Narratives “go viral” and spread far, even worldwide, with economic impact. The 1920-21 Depression, the Great Depression of the 1930s, the so-called “Great Recession” of 2007-9 and the contentious political-economic situation of today, are considered as the results of the popular narratives of their respective times. Though these narratives are deeply human …

Consequences Of The Clean Water Act And The Demand For Water Quality, David A. Keiser, Joseph S. Shapiro

Cowles Foundation Discussion Papers

Since the 1972 U.S. Clean Water Act, government and industry have invested over $1 trillion to abate water pollution, or $100 per person-year. Over half of U.S. stream and river miles, however, still violate pollution standards. We use the most comprehensive set of files ever compiled on water pollution and its determinants, including 50 million pollution readings from 170,000 monitoring sites, to study water pollution’s trends, causes, and welfare consequences. We have three main findings. First, water pollution concentrations have fallen substantially since 1972, though were declining at faster rates before then. Second, the Clean Water Act’s grants to municipal …

Consequences Of The Clean Water Act And The Demand For Water Quality, David A. Keiser, Joseph S. Shapiro

Cowles Foundation Discussion Papers

Since the 1972 U.S. Clean Water Act, government and industry have invested over $1 trillion to abate water pollution, or $100 per person-year. Over half of U.S. stream and river miles, however, still violate pollution standards. We use the most comprehensive set of files ever compiled on water pollution and its determinants, including 50 million pollution readings from 240,000 monitoring sites and a network model of all U.S. rivers, to study water pollution’s trends, causes, and welfare consequences. We have three main findings. First, water pollution concentrations have fallen substantially. Between 1972 and 2001, for example, the share of waters …

Development Of A High Throughput (Ht) Raman Spectroscopy Method For Rapid Screening Of Liquid Blood Plasma From Prostate Cancer Patients, Dinesh Medipally, Adrian Maguire, Jane Bryant, John Armstrong, Mary Dunne, Marie Finn, Fiona Lyng, Aidan Meade

Articles

Extensive research has been undertaken on the examination of tissue biopsies using vibrational spectroscopic techniques. However, fewer studies have focused on less invasive and commonly acquired blood samples. Recent studies have shown the ability of Raman and Fourier transform infrared (FTIR) spectroscopy to discriminate between non-cancer controls and cancer cases using blood serum or plasma. Even though many studies have proposed Raman spectroscopy as a potential diagnostic tool in various cancers, the Raman spectroscopic technique has not been introduced as a routine clinical technology. This is due to multiple drawbacks with the application of the technique, including sample preparation, the …

Development Of A High Throughput (Ht) Raman Spectroscopy Method For Rapid Screening Of Liquid Blood Plasma From Prostate Cancer Patients, Dinesh Medipally, Adrian Maguire, Jane Bryant, John Armstrong, Mary Dunne, Marie Finn, Fiona Lyng, Aidan Meade

Articles

Extensive research has been undertaken on the examination of tissue biopsies using vibrational spectroscopic techniques. However, fewer studies have focused on less invasive and commonly acquired blood samples. Recent studies have shown the ability of Raman and Fourier transform infrared (FTIR) spectroscopy to discriminate between non-cancer controls and cancer cases using blood serum or plasma. Even though many studies have proposed Raman spectroscopy as a potential diagnostic tool in various cancers, the Raman spectroscopic technique has not been introduced as a routine clinical technology. This is due to multiple drawbacks with the application of the technique, including sample preparation, the …

The "Mane" Muscles: Understanding The Biomechanics Of Equestrian Riding Through Muscles And Physics, Jayla Johnson

The Synapse: Intercollegiate science magazine

No abstract provided.

Translating Science Symposium: Career And Networking Panel Discussion, Victoria Albacete

The Synapse: Intercollegiate science magazine

No abstract provided.

Cigarette Smoking And Cardio-Renal Events In Patients With Atherosclerotic Renal Artery Stenosis., Christopher A Drummond, Pamela S Brewster, Wencan He, Kaili Ren, Yanmei Xie, Katherine Tuttle, Steven T Haller, Kenneth Jamerson, Lance D Dworkin, Donald E Cutlip, Timothy P Murphy, Ralph B D'Agostino, William L Henrich, Jiang Tian, Joseph I Shapiro, Christopher J Cooper

Articles, Abstracts, and Reports

Cigarette smoking causes cardiovascular disease and is associated with poor kidney function in individuals with diabetes mellitus and primary kidney diseases. However, the association of smoking on patients with atherosclerotic renal artery stenosis has not been studied. The current study utilized data from the Cardiovascular Outcomes in Renal Atherosclerotic Lesions (CORAL, NCT00081731) clinical trial to evaluate the effects of smoking on the risk of cardio-renal events and kidney function in this population. Baseline data showed that smokers (n = 277 out of 931) were significantly younger at enrollment than non-smokers (63.3±9.1 years vs 72.4±7.8 years; p

Role Of Epigenetic Modification And Immunomodulation In A Murine Prostate Cancer Model, Jay E. Sulek, Samuel P. Robinson, Albert A. Petrossian, Shaoqing Zhou, Ekaterine Goliadze, Masoud H. Manjili, Amir Toor, Georgi Guruli

Surgery Publications

INTRODUCTION. Decreased expression of highly immunogenic cancer-testis antigens (CTA) might help tumor to achieve low immunogenicity, escape immune surveillance and grow unimpeded. Our aim was to evaluate CTA expression in tumor and normal tissues and to investigate possible means of improving the immune response in a murine prostate cancer (CaP) model by using the combination of epigenetic modifier 5-azacitidine (5-AzaC) and immunomodulator lenalidomide. No study to date has examined the effect of this combination on the prostate cancer or its impact on antigen-presenting cells (APC).

MATERIALS AND METHODS. Gene microarrays were performed to compare expression of several CTA in murine …

Insights Into Local Tumor Microenvironment Immune Factors Associated With Regression Of Cutaneous Melanoma Metastases By, Junbao Yang, Maris S Jones, Romela Irene Ramos, Alfred A Chan, Agnes F Lee, Leland J Foshag, Peter A Sieling, Mark Faries, Delphine J Lee

Articles, Abstracts, and Reports

No abstract provided.

Visionary Fall 2017, College Of Optometry

Visionary

No abstract provided.

Conceptualizations Of Students With And Without Disabilities As Mathematical Problem Solvers In Educational Research; A Critical Review, Rachel Lambert, Paulo Tan

Education Faculty Articles and Research

Students with disabilities are often framed as “the problem” and have limited opportunities to engage in standards based mathematics leading to persistent underachievement. In this paper, we investigate a research divide between mathematics educational research for students with and without disabilities, a divide with significant differences in the theoretical orientations and research methodologies used to understand learners. Based on an analysis of 149 mathematics educational research articles published between 2013 and 2015, we found significant differences between articles focused on learners with and without disabilities. For those with disabilities, mathematical problem solving was understood primarily from behavioral and information processing …

A Global Vaccine Injury Compensation System, Sam F. Halabi, Saad B. Ommer

Faculty Publications

Vaccines are extremely safe and harm is rare. Worldwide, more than 30000 vaccine doses are delivered per second through routine immunization programs, which, in turn, prevent an estimated 2 million to 3 million deaths annually. Yet the specter of vaccine injury plays a central role in vaccine access and will continue to do so as vaccine technologies evolve.

Zika, Pregnancy, And The Law, Sam F. Halabi

Faculty Publications

This Essay situates a crucial component of the public health response to Zika - the effort to develop a safe and effective vaccine - within the broader literature. It does so in an effort to highlight the need to revisit the relationship between law and pregnancy - not only in the areas legal scholars have prioritized so far, but also in the context of routine and emergency maternal health, which has heretofore been largely assumed to be governed by straightforward norms and practices based on medical evidence and physician ethics. In fact, whereas the current literature tends to assume or …

Book Review: Divergent Paths: The Academy And The Judiciary, S. I. Strong

Faculty Publications

Judge Richard Posner's most recent book, Divergent Paths: The Academy and the Judiciary touches on a number of important issues, but the most revolutionary element involves Judge Posner's discussion of how the legal academy can assist with the education of current and future judges.

The Autocrine Role Of Proteoglycan-4 (Prg4) In Modulating Osteoarthritic Synoviocyte Proliferation And Expression Of Matrix Degrading Enzymes, Ali Alquraini, Maha Jamal, Ling Zhang, Tannin Schmidt, Gregory D. Jay, Khaled A. Elsaid

Pharmacy Faculty Articles and Research

Background: Lubricin/proteoglycan 4 (PRG4) is a mucinous glycoprotein secreted by synovial fibroblasts and superficial zone chondrocytes. Recently, we showed that recombinant human PRG4 (rhPRG4) is a putative ligand for CD44 receptor. rhPRG4-CD44 interaction inhibits cytokine-induced rheumatoid arthritis synoviocyte proliferation. The objective of this study is to decipher the autocrine function of PRG4 in regulating osteoarthritic synoviocyte proliferation and expression of catabolic and pro-inflammatory mediators under basal and interleukin-1 beta (IL-1β)- stimulated conditions.

Methods: Cytosolic and nuclear levels of nuclear factor kappa B (NFκB) p50 and p65 subunits in Prg4+/+ and Prg4-/- synoviocytes were studied using western blot. Nuclear …