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Narratives About Energy, Megaprojects, And The Ecology Of Tropical Rivers: The Baram River Dam Project, Marc Los Huertos

EnviroLab Asia

The conflict between development goals to build dams for hydroelectricity and indigenous peoples in Sarawak was set in motion in the 1970s. In spite of the potential ecological damage, hydroelectric development has been justified by developed and developing countries for decades. These impacts include changes in river geomorphology, water quality, and habitat value and access. Moreover, in the Bakun and Baram river watersheds, the Dayak people of Sarawak have poignantly demonstrated the socio-ecological disruption. For the time being, the construction of the Baram Dam has been halted.

University Of Richmond Sustainability Report, Robert Andrejewski

Reports

The 2016 University of Richmond Sustainability Report represents where we are now, recognizes significant accomplishments, and provides a launching point for future endeavors. We all have a role to play in the continued stewardship of this great place.

This document presents a summary of the data we collected. It serves the dual role of providing information on the considerable sustainability efforts underway and establishes a benchmark for our efforts as we move forward. There is much to be celebrated, and there is much to be done.

College Of Arts And Sciences Magazine: 2017, College Of Arts And Sciences

College of Arts and Sciences news

• WMU’s New President
• Arts and Sciences Fulbright Scholars
• Sports Media Students Work With ESPN3
• Interns Strike Gold at Michigan State Capitol and Beyond
• The Kalamazoo Autism Center, One Year Later
• Dr. Jim Olson, B.S. ‘84, Advances Cancer Treatment Through Scorpion Venom
• Dr. Jeffrey Angles Wins Japan’s Most Prestigious Prize for Literature
• Bringing Students One Step Closer to Graduation
• Unexpected Award Benefits Nearly 100 Students
• Geology and Mineral Museum to Undergo Renovation
• Congratulations to the 2016 Honorees

Center For Grassland Studies, Volume 23, Issue 4, Winter 2017

Center for Grassland Studies: Newsletters

Impacts of Cattle Grazing Corn Residue

Director's Comments

GLS Alum Named Outstanding Young Range Professional

Full Schedule for Briske, 2017 Leu Distinguished Lecturer

Internship Presentations Provide Insight

Grassland Management Graduate Certificate Program

Common Core And Stem Opportunities, Lane H. Walker, Helene J. Sherman

The Mathematics Enthusiast

There is an increasing need for educators at all levels to equip more students with problem-solving skills that better fit our changing work force. Students are largely unaware of many science-, technology-, engineering-, and math-related (STEM) careers. They often do not understand the importance of those careers or what skills are required to pursue them. Students are exposed to some of those skills if they take Career Technical Education (CTE) classes, but rarely do they see the connections in their core math classes. Math teachers have pointed to their dense curricula as making STEM integration impractical. A study of the …

Restoring Tides To Reduce Methane Emissions In Impounded Wetlands: A New And Potent Blue Carbon Climate Change Intervention, Kevin D. Kroeger, Stephens Crooks, Serena Moseman-Valtierra, Jianwu Tang

Biological Sciences Faculty Publications

Coastal wetlands are sites of rapid carbon (C) sequestration and contain large soil C stocks. Thus, there is increasing interest in those ecosystems as sites for anthropogenic greenhouse gas emission offset projects (sometimes referred to as “Blue Carbon”), through preservation of existing C stocks or creation of new wetlands to increase future sequestration. Here we show that in the globally-widespread occurrence of diked, impounded, drained and tidally-restricted salt marshes, substantial methane (CH₄) and CO₂ emission reductions can be achieved through restoration of disconnected saline tidal flows. Modeled climatic forcing indicates that tidal restoration to reduce emissions has …

Genomic Adaptation To Polyphagy And Insecticides In A Major East Asian Noctuid Pest, Tingcai Cheng, Jaiqi Wu, Yuqian Wu, Rajendra V. Chilukuri, Lihua Huang, Kohji Yamamoto, Li Feng, Wanshun Li, Zhiwei Chen, Huizhen Guo, Jianqiu Liu, Shenglong Li, Xiaoxiao Wang, Li Peng, Duolian Liu, Youbing Guo, Bohua Fu, Zhiqing Li, Chun Liu, Yuhui Chen, Archana Tomar, Frederique Hilliou, Nicolas Montagné, Emmanuelle Jacquin-Joly, Emmanuelle D'Alençon, Rakesh K. Seth, Raj K. Bhatnagar, Akiya Jouraku, Takahiro Shiotsuki, Keiko Kadono-Okuda, Amornrat Promboon, Guy Smagghe, Kallare P. Arunkumar, Hirohisa Kishino, Marian R. Goldsmith, Qili Feng, Qingyou Xia, Kazuei Mita

Biological Sciences Faculty Publications

The tobacco cutworm, Spodoptera litura, is among the most widespread and destructive agricultural pests, feeding on over 100 crops throughout tropical and subtropical Asia. By genome sequencing, physical mapping and transcriptome analysis, we found that the gene families encoding receptors for bitter or toxic substances and detoxification enzymes, such as cytochrome P450, carboxylesterase and glutathione-S-transferase, were massively expanded in this polyphagous species, enabling its extraordinary ability to detect and detoxify many plant secondary compounds. Larval exposure to insecticidal toxins induced expression of detoxification genes, and knockdown of representative genes using short interfering RNA (siRNA) reduced larval survival, …

Pretty Picky For A Generalist: Impacts Of Toxicity And Nutritional Quality On Mantid Prey Processing, Jamie L. Rafter, Justin F. Vendettuoli, Liahna Gonda-King, Daniel Niesen, Navindra P. Seeram, Chad M. Rigsby, Evan L. Preisser

Biological Sciences Faculty Publications

Prey have evolved a number of defenses against predation, and predators have developed means of countering these protective measures. Although caterpillars of the monarch butterfly, Danaus plexippus L., are defended by cardenolides sequestered from their host plants, the Chinese mantid Tenodera sinensis Saussure guts the caterpillar before consuming the rest of the body. We hypothesized that this gutting behavior might be driven by the heterogeneous quality of prey tissue with respect to toxicity and/or nutrients. We conducted behavioral trials in which mantids were offered cardenolide-containing and cardenolide-free D. plexippus caterpillars and butterflies. In addition, we fed mantids starved and unstarved …

A Multigenerational Field Experiment On Eco-Evolutionary Dynamics Of The Influential Lizard Anolis Sagrei: A Mid-Term Report, Thomas W. Schoener, Jason J. Kolbe, Manuel Leal, Jonathan B. Losos, David A. Spiller

Biological Sciences Faculty Publications

Only a handful of multi-generational experiments in natural systems of eco-evolutionary dynamics currently exist, despite Fussmann et al.'s call for more such studies nearly a decade ago. To perform such a study, in 2008 we introduced the lizard Leiocephalus carinatus, a predator (and possible food competitor) of the lizard Anolis sagrei, to seven islands having A. sagrei, with seven unmanipulated islands having A. sagrei as controls. Almost immediately, L. carinatus, which is larger and more terrestrial than A. sagrei, caused a major habitat shift in A. sagrei away from the ground and toward higher and …

Middle School Teacher Perspectives Of Ldc In A Rural Southeastern North Carolina School District, Adrain Shavonne Batten

Education Dissertations and Projects

The mixed-method phenomenological study surveyed middle school teacher perceptions of the Literacy Design Collaborative (LDC). The study population consisted of 16 sixth through eighth grade English language arts, science, or social studies teachers. The data for this study were analyzed from the 2011-2012 school year until the 2015-2016 year. The researcher utilized Stufflebeam’s (2003) context, input, process, product (CIPP) model to create the research questions. The following research questions guided the study: (1) To what extent does the LDC model influence middle school teacher decisions in the type of the instructional strategies they choose to use in their classrooms; (2) …

Catalog Denison University 2017-2018

Denison University Catalogs

Denison University Course 2017-2018

Arc Moments, Logan T. Collins

HUMBUG

No abstract provided.

Special Topic 3, Part 1: Pesticides: Natural And Manmade Pesticides, Gordon Gribble

Chemistry 52, Gordon Gribble lectures on Special Topics

No abstract provided.

Characterizing A Small-Scale, Constructed Wetland For Stormwater Treatment, Madeline Pritchett, Yuezhi Yuan

Rose-Hulman Undergraduate Research Publications

Because of the efficient treatment processes of wetlands, engineered treatment wetlands are increasingly being used to treat stormwater and wastewater, and especially combined sewer overflows (CSO). Constructed treatment wetlands are low-cost, require minimal maintenance, can be implemented in a decentralized fashion, and contribute to ecosystem preservation. All of these reasons have brought treatment wetlands to the forefront for consideration by communities working to reduce CSOs and improve water quality, especially in small cities with limited resources.

Engineering News, Winter 2017, School Of Engineering

Engineering News

No abstract provided.

Producing Sacredness And Defending Secularity: Faith In The Workplace Of Taiwanese Scientists, Di Di, Elaine Howard Ecklund

Sociology

Although a recent body of scholarship focuses on how business professionals infuse spiritual practices in their workplaces, comparatively little attention has been paid to faith in the scientific workplace, especially in an Eastern, non-Christian context. Between 2014 and 2015, we conducted a survey of 892 scientists in Taiwan and completed interviews with 52 of our survey respondents. In this paper, we examine how scientists navigate religion in the scientific workplace. Survey results demonstrate that while scientists perceive religion and scientific research as generally separate in the abstract, in practice, they regard the boundary between religion and their workplace as somewhat …

A Catholic Science? Italian Scientistsconstruct Religious Identity During Religious Shifts, Elaine Howard Ecklund, Di Di

Sociology

Scholars have argued that learning science is linked to a decrease in personal faith among scientists. We do not know much, however, about the so-called secularizing effect of science among scientists outside the US, where such religious processes could operate differently. Because the negotiation between science and religion is more salient when faith is in transition, we examine how scientists in Italy (a predominantly Catholic context) construct religious identity during religious shifts. Drawing from interviews with 81 Italian physicists and biologists, we ask whether scientists have experienced any religious shifts and how they went through these shifts, addressing personal secularization …

Distribution Bias Analysis Of Germline And Somatic Single-Nucleotide Variations That Impact Protein Functional Site And Neighboring Amino Acids, Y Pan, C Yan, Y Hu, Y Fan, Qing Pan, Q Wan, J Torcivia-Rodriguez, Raja Mazumder

Biochemistry and Molecular Medicine Faculty Publications

Single nucleotide variations (SNVs) can result in loss or gain of protein functional sites. We analyzed the effects of SNVs on enzyme active sites, ligand binding sites, and various types of post translational modification (PTM) sites. We found that, for most types of protein functional sites, the SNV pattern differs between germline and somatic mutations as well as between synonymous and non-synonymous mutations. From a total of 51,138 protein functional site affecting SNVs (pfsSNVs), a pan-cancer analysis revealed 142 somatic pfsSNVs in five or more cancer types. By leveraging patient information for somatic pfsSNVs, we identified 17 loss of functional …

Metabolic Reprogramming Of Triple-Negative Breast Cancer: The Role Of Mirnas, Amal Qattan

Biochemistry and Molecular Medicine Faculty Publications

MicroRNAs (miRNAs) are well known to influence the expression of the genes that regulate critical cellular functions. Various reports have suggested that they play critical roles in breast cancer metabolism through the regulation of various metabolic pathways, including the metabolism of glucose, lipids, glycolysis and the mitochondrial tricarboxylic acid cycle (TCA). miRNAs regulate the metabolic process by targeting key molecules (enzymes, kinases transporters) or by modifying the expression of key transcription molecules. In addition, miRNAs can indirectly regulate mRNA translation by targeting chromatin-remodeling enzymes. Furthermore, miRNAs influence the expression of both oncogenes and tumor suppressors and have a major impact …

Genomes Of Fasciola Hepatica From The Americas Reveal Colonization With Neorickettsia Endobacteria Related To The Agents Of Potomac Horse And Human Sennetsu Fevers., Samantha N Mcnulty, Jose F Tort, Gabriel Rinaldi, Bruce A Rosa, Victoria H. Mann, Patricia S. Latham, Paul J. Brindley, +Several Additional Authors

Microbiology, Immunology, and Tropical Medicine Faculty Publications

Food borne trematodes (FBTs) are an assemblage of platyhelminth parasites transmitted through the food chain, four of which are recognized as neglected tropical diseases (NTDs). Fascioliasis stands out among the other NTDs due to its broad and significant impact on both human and animal health, as Fasciola sp., are also considered major pathogens of domesticated ruminants. Here we present a reference genome sequence of the common liver fluke, Fasciola hepatica isolated from sheep, complementing previously reported isolate from cattle. A total of 14,642 genes were predicted from the 1.14 GB genome of the liver fluke. Comparative genomics indicated that F. …

T-Cell Responses In Individuals Infected With Zika Virus And In Those Vaccinated Against Dengue Virus, Dominic Paquin-Proulx, Douglas F. Nixon, +Several Additional Authors

Microbiology, Immunology, and Tropical Medicine Faculty Publications

Background: The outbreak of Zika virus (ZIKV) infection in Brazil has raised concerns that infection during pregnancy could cause microcephaly and other severe neurodevelopmental malformations in the fetus. The mechanisms by which ZIKV causes fetal abnormalities are largely unknown. The importance of pre-infection with dengue virus (DENV), or other flaviviruses endemic to Brazil, remains to be investigated. It has been reported that antibodies directed against DENV can increase ZIKV infectivity by antibody dependent enhancement (ADE), suggesting that a history of prior DENV infection might worsen the outcome of ZIKV infection.

Methods: We used bioinformatics tools to design 18 peptides from …

Inhibiting Dna Methylation Activates Cancer Testis Antigens And Expression Of The Antigen Processing And Presentation Machinery In Colon And Ovarian Cancer Cells., Cornelia Siebenkäs, Katherine B Chiappinelli, Angela A Guzzetta, Anup Sharma, Jana Jeschke, Rajita Vatapalli, Stephen B Baylin, Nita Ahuja

Microbiology, Immunology, and Tropical Medicine Faculty Publications

Innovative therapies for solid tumors are urgently needed. Recently, therapies that harness the host immune system to fight cancer cells have successfully treated a subset of patients with solid tumors. These responses have been strong and durable but observed in subsets of patients. Work from our group and others has shown that epigenetic therapy, specifically inhibiting the silencing DNA methylation mark, activates immune signaling in tumor cells and can sensitize to immune therapy in murine models. Here we show that colon and ovarian cancer cell lines exhibit lower expression of transcripts involved in antigen processing and presentation to immune cells …

Enriched Environment And Stress Exposure Influence Splenic B Lymphocyte Composition., Blake T Gurfein, Burcu Hasdemir, Jeffrey M Milush, Chadi Touma, Rupert Palme, Douglas F Nixon, Nicholas Darcel, Frederick M Hecht, Aditi Bhargava

Microbiology, Immunology, and Tropical Medicine Faculty Publications

Prolonged chronic stress has deleterious effects on immune function and is associated with numerous negative health outcomes. The spleen harbors one-fourth of the body’s lymphocytes and mediates both innate and adaptive immune responses. However, the subset of splenic lymphocytes that respond, either adaptively or maladaptively, to various stressors remains largely unknown. Here we investigated the effects of unpredictable chronic mild stress (CMS) exposure on spleen composition in male mice housed in two different caging conditions: standard caging (Cntl) and enriched environment (EE). EE-caged mice exhibited the greatest absolute number of splenocytes and CMS exposure significantly lowered splenocyte numbers in both …

Fit For Genomic And Proteomic Purposes: Sampling The Fitness Of Nucleic Acid And Protein Derivatives From Formalin Fixed Paraffin Embedded Tissue., Anna Yakovleva, Jordan L Plieskatt, Sarah Jensen, Razan Humeida, Jonathan Lang, Guangzhao Li, Paige Bracci, Sylvia Silver, Jeffrey Michael Bethony

Microbiology, Immunology, and Tropical Medicine Faculty Publications

The demand for nucleic acid and protein derivatives from formalin-fixed paraffin-embedded (FFPE) tissue has greatly increased due to advances in extraction and purification methods, making these derivatives available for numerous genomic and proteomic platforms. Previously, DNA, RNA, microRNA (miRNA), or protein derived from FFPE tissue blocks were considered “unfit” for such platforms, as the process of tissue immobilization by FFPE resulted in cross-linked, fragmented, and chemically modified macromolecules. We conducted a systematic examination of nucleic acids and proteins co-extracted from 118 FFPE blocks sampled from the AIDS and Cancer Specimen Resource (ACSR) at The George Washington University after stratification by …

A Modified Fasp Protocol For High-Throughput Preparation Of Protein Samples For Mass Spectrometry., Jeremy Potriquet, Marut Laohaviroj, Jeffrey M Bethony, Jason Mulvenna

Microbiology, Immunology, and Tropical Medicine Faculty Publications

o facilitate high-throughput proteomic analyses we have developed a modified FASP protocol which improves the rate at which protein samples can be processed prior to mass spectrometry. Adapting the original FASP protocol to a 96-well format necessitates extended spin times for buffer exchange due to the low centrifugation speeds tolerated by these devices. However, by using 96-well plates with a more robust polyethersulfone molecular weight cutoff membrane, instead of the cellulose membranes typically used in these devices, we could use isopropanol as a wetting agent, decreasing spin times required for buffer exchange from an hour to 30 minutes. In a …

Schistosoma Mansoni Reinfection: Analysis Of Risk Factors By Classification And Regression Tree (Cart) Modeling., Andréa Gazzinelli, Roberta Oliveira-Prado, Leonardo Ferreira Matoso, Bráulio M Veloso, Gisele Andrade, Helmut Kloos, Jeffrey M Bethony, Renato M Assunção, Rodrigo Correa-Oliveira

Microbiology, Immunology, and Tropical Medicine Faculty Publications

Praziquantel (PZQ) is an effective chemotherapy for schistosomiasis mansoni and a mainstay for its control and potential elimination. However, it does not prevent against reinfection, which can occur rapidly in areas with active transmission. A guide to ranking the risk factors for Schistosoma mansoni reinfection would greatly contribute to prioritizing resources and focusing prevention and control measures to prevent rapid reinfection. The objective of the current study was to explore the relationship among the socioeconomic, demographic, and epidemiological factors that can influence reinfection by S. mansoni one year after successful treatment with PZQ in school-aged children in Northeastern Minas …

Mycobacterium Tuberculosis Reactivates Latent Hiv-1 In T Cells In Vitro, Erica Larson, Camille Novis, Laura Martins, Amanda Macedo, Kadyn Kimball, Alberto Bosque, Vincent Planelles, Louis Barrows

Microbiology, Immunology, and Tropical Medicine Faculty Publications

Following proviral integration into the host cell genome and establishment of a latent state, the human immunodeficiency virus type 1 (HIV-1) can reenter a productive life cycle in response to various stimuli. HIV-1 reactivation occurs when transcription factors, such as nuclear factor-κB (NF-κB), nuclear factor of activated T cells (NFAT), and activator protein -1 (AP-1), bind cognate sites within the long terminal repeat (LTR) region of the HIV-1 provirus to promote transcription. Interestingly, pattern recognition receptors (PRRs) that recognize pathogen-associated molecular patterns (PAMPs) can reactivate latent HIV-1 through activation of the transcription factor NF-κB. Some PRRs are expressed on central …

Ancylostoma Ceylanicum Infective Third-Stage Larvae Are Activated By Co-Culture With Ht-29-Mtx Intestinal Epithelial Cells, Caitlin Feather, John M. Hawdon, John March

Microbiology, Immunology, and Tropical Medicine Faculty Publications

Background

Human hookworm larvae arrest development until they enter an appropriate host. This makes it difficult to access the larvae for studying larval development or host-parasite interactions. While there are in vivo and in vitro animal models of human hookworm infection, there is currently no human, in vitro model. While animal models have provided much insight into hookworm biology, there are limitations to how closely this can replicate human infection. Therefore, we have developed a human, in vitro model of the initial phase of hookworm infection using intestinal epithelial cell culture.

Results

Co-culture of the human hookworm Ancylostoma ceylanicum with …

Fusion, 2017, George Washington University, William H. Beaumont Medical Research Honor Society

Fusion

No abstract provided.

Dbvar Structural Variant Cluster Set For Data Analysis And Variant Comparison, Ben Busby, Lon Phan, Jeffrey Hsu, Le Quang Minh Tri, Micheala Willi, Tamer A. Mansour, Yan Kai, John R. Garner, John R. Lopez

Anatomy and Regenerative Biology Faculty Publications

dbVar houses over 3 million submitted structural variants (SSV) from 120 human studies including copy number variations (CNV), insertions, deletions, inversions, translocations, and complex chromosomal rearrangements. Users can submit multiple SSVs to dbVAR that are presumably identical, but were ascertained by different platforms and samples, to calculate whether the variant is rare or common in the population and allow for cross validation. However, because SSV genomic location reporting can vary – including fuzzy locations where the start and/or end points are not precisely known – analysis, comparison, annotation, and reporting of SSVs across studies can be difficult. This project was …