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Full-Text Articles in Entire DC Network
An Approach To Building Musical Bioprocessors With Physarum Polycephalum Memristors, E Braund, Er Miranda, Er Miranda
An Approach To Building Musical Bioprocessors With Physarum Polycephalum Memristors, E Braund, Er Miranda, Er Miranda
School of Art, Design and Architecture
No abstract provided.
On Unconventional Computing For Sound And Music, Er Miranda, A Kirke, E Braund, A Antoine
On Unconventional Computing For Sound And Music, Er Miranda, A Kirke, E Braund, A Antoine
School of Art, Design and Architecture
No abstract provided.
Tidal Conversion And Mixing Poleward Of The Critical Latitude (An Arctic Case Study), Tom P. Rippeth, Vasiliy Vlasenko, Nataliya Stashchuk, Brian D. Scannell, J. A.Mattias Green, Ben J. Lincoln, Sheldon Bacon
Tidal Conversion And Mixing Poleward Of The Critical Latitude (An Arctic Case Study), Tom P. Rippeth, Vasiliy Vlasenko, Nataliya Stashchuk, Brian D. Scannell, J. A.Mattias Green, Ben J. Lincoln, Sheldon Bacon
School of Biological and Marine Sciences
The tides are a major source of the kinetic energy supporting turbulent mixing in the global oceans. The prime mechanism for the transfer of tidal energy to turbulent mixing results from the interaction between topography and stratified tidal flow, leading to the generation of freely propagating internal waves at the period of the forcing tide. However, poleward of the critical latitude (where the period of the principal tidal constituent exceeds the local inertial period), the action of the Coriolis force precludes the development of freely propagating linear internal tides. Here we focus on a region of sloping topography, poleward of …
Sexual Health Education For Behavior Change: How Much Is Enough?, Ross Shegog, Elizabeth Baumler, Robert C. Addy, Melissa Peskin, Melanie Ann Thiel, Susan R. Tortolero, Christine Markham
Sexual Health Education For Behavior Change: How Much Is Enough?, Ross Shegog, Elizabeth Baumler, Robert C. Addy, Melissa Peskin, Melanie Ann Thiel, Susan R. Tortolero, Christine Markham
Journal of Applied Research on Children: Informing Policy for Children at Risk
Purpose: Successful implementation of sexual health curricula in school settings is often compromised by competing academic priorities. This study explores the association between exposure to sexual health lessons (time-on-task in hours and lesson content topics) and delayed sexual initiation of middle school students at long term follow-up.
Methods: Post hoc data analysis was conducted from a RCT (n=15 middle schools) in the south-central U.S. in which grade 7 students demonstrated delayed sexual initiation (adjusted odds ratio [AOR]: 1.54, 95% CI: 1.20 to 1.99) by grade 9 follow-up after using It’s Your Game (IYG), a 24 lesson sexual health curriculum. Logistic …
Native Iyg: Improving Psychosocial Protective Factors For Hiv/Sti And Teen Pregnancy Prevention Among Youth In American Indian/Alaska Native Communities, Ross Shegog Phd., Stephanie Craig Rushing Phd, Mph, Cornelia Jessen Ma, Travis L. Lane Ba, Gwenda Gorman Bs, Amanda Gaston Mat, Taija Koogei Revels Bs, Jennifer Torres Mph, Jennifer Williamson, Elizabeth Baumler Phd., Robert C. Addy Phd., Melissa F. Peskin Phd., Susan R. Tortolero, Christine M. Markham Phd.
Native Iyg: Improving Psychosocial Protective Factors For Hiv/Sti And Teen Pregnancy Prevention Among Youth In American Indian/Alaska Native Communities, Ross Shegog Phd., Stephanie Craig Rushing Phd, Mph, Cornelia Jessen Ma, Travis L. Lane Ba, Gwenda Gorman Bs, Amanda Gaston Mat, Taija Koogei Revels Bs, Jennifer Torres Mph, Jennifer Williamson, Elizabeth Baumler Phd., Robert C. Addy Phd., Melissa F. Peskin Phd., Susan R. Tortolero, Christine M. Markham Phd.
Journal of Applied Research on Children: Informing Policy for Children at Risk
Background: Few HIV/STI and pregnancy prevention programs for youth in American Indian and Alaska Native (AI/AN) communities have been rigorously evaluated despite sexual health disparities in this population. This study reports the evaluation of a culturally adapted Internet-based HIV/STI and pregnancy prevention program for AI/AN youth, Native It’s Your Game (Native IYG).
Methods: A randomized study was conducted with 523 youth (12 to 14 years old), recruited from 25 tribal sites in Alaska, Arizona, and the Pacific Northwest. Participants were surveyed at baseline and upon completion of treatment or comparison interventions. Multivariable linear regression models were used to assess impact …
Medical School Watercooler Newsletter - December 3, 2017, Lindsey Lyle
Medical School Watercooler Newsletter - December 3, 2017, Lindsey Lyle
Watercooler Newsletter
This is the December 3, 2017 edition of the Frederick P. Whiddon College of Medicine's Newsletter - Watercooler.
Contents Include:
Pediatrics Hosting Grand Rounds on Dec. 15
'It Has Been a Pleasure to Serve'
Dr. Elizabeth Minto Honored by National Multiple Sclerosis Society
Dr. Mohammed Berrou Presents at 13th Annual Respiratory Disease Young Investigators’ Forum
Anthony Beck Named Director of Operations for Family Medicine
Acquisition Of Zetasizer Enhances Nanotechnology Research Ohio, Ohio University College Of Arts & Sciences
Acquisition Of Zetasizer Enhances Nanotechnology Research Ohio, Ohio University College Of Arts & Sciences
All Forum Articles
No abstract provided.
The Family History Of Austin B. Stapleton, Austin Stapleton
The Family History Of Austin B. Stapleton, Austin Stapleton
Your Family in History: HIST 550/700
This document is associated with genealogical findings and research conducted by Austin B. Stapleton, in order to complete requirements for Your Family in History 550 at Pittsburg State University. This research includes information compiled over the fall semester of 2017 and is a cumulative product of a social family history for Austin's ancestors in and around the Joplin, Missouri, area and beyond.
The Acquisition Of Syntax: A Nativist Perspective Vs. A Cognitivist Perspective, Rashid Al-Balush
The Acquisition Of Syntax: A Nativist Perspective Vs. A Cognitivist Perspective, Rashid Al-Balush
Journal of Arts and Social Sciences
This paper looks into how two main frameworks view the acquisition of syntax. These are the nativist approach which claims that language is acquired because human beings are equipped with a language acquisition device in the form of grammatical knowledge, and the cognitivist approach which views language like the other cognitive skills and so claims that we acquire language using general cognitive mechanisms that are not specific to language. The paper reviews a number of studies that report on the acquisition of various aspects of the syntax of natural language; the studies are from both frameworks. The paper then evaluates …
Morphological Study Of Pollen Grains And Seeds In Eight Species From The Family Cruciferae In Iraq, Rasha K.H. Al-Masoud, Khalil I.A. Al-Shammary
Morphological Study Of Pollen Grains And Seeds In Eight Species From The Family Cruciferae In Iraq, Rasha K.H. Al-Masoud, Khalil I.A. Al-Shammary
Baghdad Science Journal
The shapedimensions and characteristics of pollen grains and seeds have importance in distinguish among species.Therefore, the present study included morphological characteristics of pollen grains and seeds for eight species belonging to eight genera of the family Brassicaceae and these species are: Alliaria petiolata (M.Bieb) Cavara etGrand, Aubrieta parviflora Boiss, Cardamine hirsuta L., Crambe orientalis L., Eromobium aegyptiacum (Spreng.) Schweinf.et Asch.ex Boiss., Parlatoria cakiloideaBoiss., Sterigmostemum sulphureum (Banksetsol.) Bornm. Neotorularia torulosa(Desf.)Hedge&J.Leonard. The pollen grains were studied in morphological and full measurements were taken, the study showed that the majority of the pollen grains was tricolpate and shape was determined to be prolate, …
Community Structure Of Benthic Algae In A Lotic Ecosystem, Karbala Province-Iraq, F. M. Hassan, J. M Salman, S. Al-Nasrawi
Community Structure Of Benthic Algae In A Lotic Ecosystem, Karbala Province-Iraq, F. M. Hassan, J. M Salman, S. Al-Nasrawi
Baghdad Science Journal
This study focused on benthic algae (epipelic and attached algae on concrete lining stream) in Bani-Hassan stream in Holly Karbala, Iraq. The qualitative and quantitative studies of benthic algae were done by collecting 240 samples from five sites in the study area for the period from December 2012 to November 2013. Also, the environmental variables of the stream were examined in term of temporary and spatial. The results showed that the stream was alkaline, hard, oligohaline and a well aerated. The total nitrogen to the total phosphorus (TN: TP) ratio indicates nitrogen limitation. 129 species of benthic algae belonging to …
Inhibitory Effect Of Bacteriophages Isolated From Sewage Water In The City Of Kirkuk On Some Types Of Human Pathogenic Bacteria, Muhsin Hamad Edham, Firas Md Al-Tae, Alaa Ty Al-Hammadi
Inhibitory Effect Of Bacteriophages Isolated From Sewage Water In The City Of Kirkuk On Some Types Of Human Pathogenic Bacteria, Muhsin Hamad Edham, Firas Md Al-Tae, Alaa Ty Al-Hammadi
Baghdad Science Journal
Most approaches to combat antibiotic resistant bacteria concentrate on discovering new antibiotics or modifying existing ones. However, one of the most promising alternatives is the use of bacteriophages. This study was focused on the isolation of bacteriophages that are specific to some of commonly human pathogens namely E. coli, Streptococcus pyogenes, Staphylococcus aureus, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella spp. and Klebsiella pneumoniae. These bacteriophages were isolated from sewages that were collected from four different locations in Kirkuk City. Apart from S. pyogenes, bacteriophages specific to all tested bacteria were successfully isolated and tested for their effectiveness by spot test. The …
Synthesis Of Silver Nanoparticles From Malva Parviflora Extract And Effect On Ecto-5'- Nucleotidase(5'-Nt), Ada And Ampda Enzymes In Sera Of Patients With Arthrosclerosis, Ahlam M. Farhan, Rasha A. Jassim, Nafeesa J. Kadhim, Wesen Adel Mehdi, Atheer Awad Mehde
Synthesis Of Silver Nanoparticles From Malva Parviflora Extract And Effect On Ecto-5'- Nucleotidase(5'-Nt), Ada And Ampda Enzymes In Sera Of Patients With Arthrosclerosis, Ahlam M. Farhan, Rasha A. Jassim, Nafeesa J. Kadhim, Wesen Adel Mehdi, Atheer Awad Mehde
Baghdad Science Journal
The present researchincluded synthesis of silver nanoparticle from(1*10-3,1*10-4and1*10-5) M aqueous AgNO3solution through the extract of M.parviflora reducing agent.In the process of synthesizing silver nanoparticles we detected a rapid reduction of silver ions leading to the formation of stable crystalline silver nanoparticles in the solution.The characteristics of silver nanoparticles were studied by using UV-Visible absorption spectroscopy, and atomic force microscope (AFM)analysis. The AFM measurements showed that the average size of silver nanoparticles synthesized using (1*10-3,1*10-4and1*10-5) M aqueous AgNO3solution through the extract of M.parviflorawere 102 to 114nm. UV-Vis spectra of the aqueous medium containing silver nanoparticles showed a surface peak at 220nm …
Hyperhomocysteinemia-Induced Gene Expression Changes In The Cell Types Of The Brain, Erica M. Weekman, Abigail E. Woolums, Tiffany L. Sudduth, Donna M. Wilcock
Hyperhomocysteinemia-Induced Gene Expression Changes In The Cell Types Of The Brain, Erica M. Weekman, Abigail E. Woolums, Tiffany L. Sudduth, Donna M. Wilcock
Sanders-Brown Center on Aging Faculty Publications
High plasma levels of homocysteine, termed hyperhomocysteinemia, are a risk factor for vascular cognitive impairment and dementia, which is the second leading cause of dementia. While hyperhomocysteinemia induces microhemorrhages and cognitive decline in mice, the specific effect of hyperhomocysteinemia on each cell type remains unknown. We took separate cultures of astrocytes, microglia, endothelial cells, and neuronal cells and treated each with moderate levels of homocysteine for 24, 48, 72, and 96 hr. We then determined the gene expression changes for cell-specific markers and neuroinflammatory markers including the matrix metalloproteinase 9 system. Astrocytes had decreased levels of several astrocytic end feet …
New Synthesis Of Cyclic Imide Compounds For Ampicillin Drug And Studying Their Biological Activity, Entesar O. Al-Tamimi, Enaam Fadil Mousa
New Synthesis Of Cyclic Imide Compounds For Ampicillin Drug And Studying Their Biological Activity, Entesar O. Al-Tamimi, Enaam Fadil Mousa
Baghdad Science Journal
Some new cyclic imides are prepared by the reaction of ampicillin drug with different cyclic anhydrides as a first step to form amic acids for ampicillin drug. The second step includes the reaction of prepared amic acids with acetic anhydride and anhydrous sodium acetate with heating in THF as a solvent to give cyclic imide compounds. These compounds are identified by melting points, FT-IR, 1H-NMR, and biological activity
Measurement Of Indoor Radon Gas Concentration In Same Region Of Baghdad Governorate Using Cr-39 Nuclear Track Detector, Ruwiadah Tarek Mahdi
Measurement Of Indoor Radon Gas Concentration In Same Region Of Baghdad Governorate Using Cr-39 Nuclear Track Detector, Ruwiadah Tarek Mahdi
Baghdad Science Journal
In this research the activity of radon gas in air in Baghad governorate,Iraq, using “alpha-emitters track registration (CR-39) track detector were measured. This measurement was done for selected areas from Baghdad Governorate, The results obtained shows that the highest average concentrations for Rn-222 is (179.077 Bq/m^3) which was recorded within Al-Shaaib city and less average concentrations was (15.79 Bq/m^3) in the nearby residential area of Baghdad International Airport and the overall average concentrations is (86.508 Bq/m^3) for these regions. Then the radon concentration was measured annual effective dose calculated from radon concentration and found in range from 0.4031 mSv/y to …
Effect Of The Silver Nanoparticles On The Histology Of Albino Mice Ovaries, Gazwa D .Al Al –Nakeeb, Noor Mahdi Fatheel
Effect Of The Silver Nanoparticles On The Histology Of Albino Mice Ovaries, Gazwa D .Al Al –Nakeeb, Noor Mahdi Fatheel
Baghdad Science Journal
The aim of study is to identify the histological changes in ovaries of the albino mice exposed to silver nanoparticles. Sixty adult females were collected and exposures by 4000 p.p.m. and 50-150 nm in size, Females were divided into 3treated groups. The concentration dosage was (1, 1.5 and 2) p.p.m. of silver nanoparticles for 7, 14 and 21 days as exposure periods as well as control group which treated by normal saline. Treated groups appeared different histopathological changes, it is depending on the concentration of silver nanoparticles and the period of exposure. These changes were included congestion in the blood …
The Efficiency Of Using Ozone Gas And Heat To Control Larvae And Adult Stage Of Red Flour Beetle, Tribolium Castaneum (Herbst) (Coleoptera: Tenebrionidae), F. A. Sabeat
Baghdad Science Journal
The effect of ozone gas with temperatures 35. 40 and 45 Celsius to control on larval and adult stages of Tribolium castaneum was tested and at time of exposure 1.0, 3.0, 7.0, 9.0, 11.0, 13.0 hours and the level of relative humidity prevailing during the test 45 ± 1%. Results showed significant differences between the exposure times, when the percentage of mortality 100% between larvae (primary, intermediate and advanced) in the treatment effect at a temperature of 35 Celsius with ozone gas at exposure time to 9.0, 11.0, 11.0 hours for the three ages respectively, and adult (male and female) …
Biological Control Of Acaudalerodes Rachipora (Singh) (Hemiptera: Alerodidae) By The Entomopathogenic Fungi On In Field, Fayhaa Abbood Mahdi Al-Nadawi
Biological Control Of Acaudalerodes Rachipora (Singh) (Hemiptera: Alerodidae) By The Entomopathogenic Fungi On In Field, Fayhaa Abbood Mahdi Al-Nadawi
Baghdad Science Journal
This study was conducted in the College of Agriculture fields /University of Baghdad, during Autumn 2013. This study was aimed to examine the mortality rate on the all black fly stages of Acaudalerodes rachipora Singh) by the biotic fungus Beuveria bassiana. The results of a preliminary survey showed that the samples of Ziziphus spaina christi were infested by blakflies in Agriculture collage during Autumn seasons of 2013 , the presence of species of black flies A. rachipora on the lower surface of the leaf, the study aimed to study and research the effects of fungus B. bassiana on black fly …
Comparison Among Populations Of Mosquitoes Culex Quinquefasciatus Say By Using Geometric Morphometric Technique From Different Regions Of Iraq, Sahar Abd, Emad Ahmed
Comparison Among Populations Of Mosquitoes Culex Quinquefasciatus Say By Using Geometric Morphometric Technique From Different Regions Of Iraq, Sahar Abd, Emad Ahmed
Baghdad Science Journal
The geometric morphometric technique was used to study the variables in the shape and size wings of different populations of mosquitoes Culex quinquefasciatus from different Iraqi provinces Babylon, Baghdad and Wasit. The results showed that the average of centroid size were 366, 387.5 and 407.4 Micron in Babylon, Baghdad and Kut, respectively. The statistical analysis showed that there were no significant differences in the average of centroid size of all specimens and they belong to the same species.
Determination Of The Level Of Some Adipokines In Hypo-And Hyperthyroids Patients In Baghdad City, Suha A. Al-Jowari
Determination Of The Level Of Some Adipokines In Hypo-And Hyperthyroids Patients In Baghdad City, Suha A. Al-Jowari
Baghdad Science Journal
Determination of the level of adipokines (obestatin, vaspin, tumor necrosis factor-α and interleukin-6)in hypo-and hyperthyroid patients from Educational Baghdad Hospital in Baghdad City was investigated. Fifty patients with hypothyroidism and Fifty patients with hyperthyroidism were selected. A control group of thirty euthyroid persons was included. Blood was collected by vein puncture and serum was separated and stored at –20C. Adipokines (obestatin,vaspin, tumor necrosis factor-αand interleukin-6) were estimated using ELISA method.The findings show a significant (p<0.05) increase in obestatin level in hypothyroid patients, while there isno significant difference in hyperthyroid patientsascompared with the euthyroid subjects. In addition, this study demonstrated a significant (p<0.05) increase in vaspin means in hypothyroid patients compared with euthyroid group. In hyperthyroid patients, there isa significant (p<0.05) decrease in vaspin level in comparison with euthyroid subjects.Furthermore, there isa significant (p<0.05) increase in tumor necrosis factor-α level in hypothyroid and hyperthyroid patients as compared with the euthyroid subjects. The results of this study also explain a significant (p<0.05) increase in interleukin-6 level in hyperthyroid patients, while there isnon-significant difference in hypothyroid patients compared with the euthyroid subjects. It is concludedfrom this studythat the levels of interleukin-6 and tumor necrosis factor-α increased in hyperthyroidism and decreased in hypothyroidism. Obestatin and vaspin were increased significantly in hypothyroid patients.
The Innovative Method For Vaccine Preparation Against Multidrug Resistant And Virulence Acinetobacter Baumannii Iraqi Isolates, Hussam Sami Awayid, Rajwa Hasen Esaa, Eman N. Naji, Meroj A. Jasem
The Innovative Method For Vaccine Preparation Against Multidrug Resistant And Virulence Acinetobacter Baumannii Iraqi Isolates, Hussam Sami Awayid, Rajwa Hasen Esaa, Eman N. Naji, Meroj A. Jasem
Baghdad Science Journal
The expanding of the medically important diseases created by multidrug-resistant Acinetobacter baumannii warrants the evolve a new methodology for prevention includes vaccination and treatment. Totally of forty-five clinical isolates identified as A.baumannii were obtained from hospitalized patients from three hospital in Baghdad City during the period from February 2016 to August 2016. Followed by diagnosing using different methods. Every strain was tested for susceptibility testing also some important virulence factorswere detected. Two isolates were chosen for the immunization and vaccine model, the first one remittent for most antibiotics except one are too virulence (strong) and the second is less virulent …
A Study Of Zooplankton Community In Dukan Lake, Kurdistan Region-Iraq, With A New Record Of Craspedacusta Sowerbii Lankester (1880) Medusa (Cnidaria: Hydrozoa), Sarah Fares Dhahir, Luay Abdul-Qader Ali
A Study Of Zooplankton Community In Dukan Lake, Kurdistan Region-Iraq, With A New Record Of Craspedacusta Sowerbii Lankester (1880) Medusa (Cnidaria: Hydrozoa), Sarah Fares Dhahir, Luay Abdul-Qader Ali
Baghdad Science Journal
A study of Zooplankton community has been carried out at four selected sites on Dukan Lake. Samples of water and zooplankton were collected monthly for the period from July 2015 to February 2016. Some physical and chemical properties of water were studied and the results showed that the air temperature were ranged from 0 to 36.16 °C, water temperature ranged from 2.83 to 34.66 °C, hydrogen ion concentration of studied sites were found to lie in alkaline side, it was ranged between 6.87 to 8.57, electrical conductivity ranged from 190.79 to 850.08 µs.cm¹, turbidity ranged from 0.9-7.7 NTU, and dissolved …
Synthesis And Characterization Of Some Metal Complexes Of [4-Methoxy-N-(Pyrimidine-2-Ylcarbamothioyl)Benzamide], Basima M . Sarhan, Bushra M. Fyyadh
Synthesis And Characterization Of Some Metal Complexes Of [4-Methoxy-N-(Pyrimidine-2-Ylcarbamothioyl)Benzamide], Basima M . Sarhan, Bushra M. Fyyadh
Baghdad Science Journal
A new ligand [4-Methoxy -N-(pyrimidine-2-ylcarbamothioyl) benzamide] (MPB) was synthesized by reactioniofi(4-Methoxyibenzoyliisothiocyanate)withi(2-aminopyri-midine). The Ligand was characterized by elemental micro analysis (C.H.N.S),(FT-IR) (UV- Vis) and (1Hi,13CNMR)spectra. Some transition metals complexes of this ligand were prepared and characterized by (FT-IR, UV-Vis) spectra conductivity measurements magnetic susceptibility and atomic absorption. From the obtained results the molecular formula of all complexes was suggested to be [M(MPB)2Cl2] (M+2i=Cu, Mn, Co ,Ni ,Zn ,Cd and Hg),the proposed geometrical structure for all complexes was an octahedral.
Synthesis New Liquid Selective Electrodes Of Ciprofloxacin Hydrochloride For Determination Ciprofloxacin In Pure Form And Pharmaceuticals Preparation., Amina M. Abass
Baghdad Science Journal
New membrane electrodes for determination of ciprofloxacin hydrochloride were prepared depending on ciprofloxacin hydrochloride - phosphotungstic acid (CFH-PT) as an active material and these electrodes were made with three plasticizers: Di-octylphenylphosphonate(DOPH), Di-butyl phosphate (DBP)Tri-n-butyl phosphate(TBP), in PVC matrix. One of the ciprofloxacin electrodes was gave Nernstian slope equal to 57.21 mV/ decade for DOPH membrane with concentration range from 1.5×10-5 to1.0×10-1 M, and detection limit equal to 1.5×10-6 M .Lifetime was 93 days. Non- Nernstian responses equal to 39.40 and 30.70 mV/ decade for membranes DBP, TBP, respectively. These electrodes were gave concentration range from 1.0× 10-5 to 1.0×10-2 and …
Preparation And Characterization Of Lead Oxide Nanoparticles By Laser Ablation As Antibacterial Agent, Halah H. Rashed, Fattin A. Fadhil, Iman H. Hadi
Preparation And Characterization Of Lead Oxide Nanoparticles By Laser Ablation As Antibacterial Agent, Halah H. Rashed, Fattin A. Fadhil, Iman H. Hadi
Baghdad Science Journal
In this work, lead oxide nanoparticles were prepared by laser ablation of lead target immersed in deionized water by using pulsed Nd:YAG laser with laser energy 400 mJ/pulse and different laser pulses. The chemical bonding of lead oxide nps was investigated by Fourier Transform Infrared (FTIR); surface morphology and optical properties were investigated by Scanning Electron Microscope (SEM) and UV-Visible spectroscopy respectively, and the size effect of lead oxide nanoparticles was studied on its antibacterial action against two types of bacteria Gram-negitive (Escherichia coli) and Gram-positive (Staphylococcusaurus) by diffusion method. The antibacterial property results show that the antibacterial activity of …
One-Step Hot Formamide Extraction Of Rna From Saccharomyces Cerevisiae, Daniel Shedlovskiy, Natalia Shcherbik, Dimitri G Pestov
One-Step Hot Formamide Extraction Of Rna From Saccharomyces Cerevisiae, Daniel Shedlovskiy, Natalia Shcherbik, Dimitri G Pestov
Rowan-Virtua School of Osteopathic Medicine Departmental Research
Current methods for isolating RNA from budding yeast require lengthy and laborious steps such as freezing and heating with phenol, homogenization with glass beads, or enzymatic digestion of the cell wall. Here, extraction with a solution of formamide and EDTA was adapted to isolate RNA from whole yeast cells through a rapid and easily scalable procedure that does not require mechanical cell lysis, phenol, or enzymes. RNA extracted with formamide-EDTA can be directly loaded on gels for electrophoretic analysis without alcohol precipitation. A simplified protocol for downstream DNase treatment and reverse transcription reaction is also included. The formamide-EDTA extraction of …
Endothelial Nitric Oxide Synthase Oxygenase On Lipid Nanodiscs: A Nano-Assembly Reflecting Native-Like Function Of Enos, Ghaith Altawallbeh, Mohammad M. Haque, Kiril A. Streletzky, Dennis J. Stuehr, Mekki Bayachou
Endothelial Nitric Oxide Synthase Oxygenase On Lipid Nanodiscs: A Nano-Assembly Reflecting Native-Like Function Of Enos, Ghaith Altawallbeh, Mohammad M. Haque, Kiril A. Streletzky, Dennis J. Stuehr, Mekki Bayachou
Physics Faculty Publications
© 2017 Elsevier Inc. Endothelial nitric oxide synthase (eNOS) is a membrane-anchored enzyme. To highlight the potential role and effect of membrane phospholipids on the structure and activity of eNOS, we have incorporated the recombinant oxygenase subunit of eNOS into lipid nanodiscs. Two different size distribution modes were detected by multi-angle dynamic light scattering both for empty nanodiscs, and nanodiscs-bound eNOSoxy. The calculated hydrodynamic diameter for mode 1 species was 9.0 nm for empty nanodiscs and 9.8 nm for nanodisc bound eNOSoxy. Spectroscopic Griess assay was used to measure the enzymatic activity. Remarkably, the specific activity of nanodisc-bound eNOSoxy is …
Masson Engineering Pumpkin-Shaped Molecular Transporters, Ohio University College Of Arts & Sciences
Masson Engineering Pumpkin-Shaped Molecular Transporters, Ohio University College Of Arts & Sciences
All Forum Articles
No abstract provided.
Meyers Plant Biology Experiment Goes To Space This Week, Ohio University College Of Arts & Sciences
Meyers Plant Biology Experiment Goes To Space This Week, Ohio University College Of Arts & Sciences
All Forum Articles
No abstract provided.