Digital Commons Network^™

Angiostoma Norvegicum N. Sp. (Nematoda: Angiostomatidae) A Parasite Of Arionid Slugs In Norway, Jenna L. Ross, Solveig Haukeland, Bjørn A. Hatteland, Elena S. Ivanova

All Peer-Reviewed Publications

Angiostoma norvegicum n. sp. (Angiostomatidae) is described from the oesophagus, crop and the buccal mass of five species of slugs of the family Arionidae, Arion vulgaris (Moquin-Tandon), Arion ater (L.), Arion fasciatus (Nilsson), Arion fuscus (Müller) and Arion rufus/Arion ater hybrid), collected throughout Norway. Angiostoma norvegicum n. sp. was found parasitising arionids at seven of the 30 sample sites examined (23.3%), and 9.9% of all Arion spp. were infected with this nematode. The new species is characterised by its large size (4.0–8.6 mm long) and in having: lateral alae; 6 + 6 papillae at the cephalic end; a large circular …

Entomopathogens Routinely Used In Pest Control Strategies: Orchards In Tropical Climate, N. K. Maniania, S. Ekesi, C. Dolinski

All Peer-Reviewed Publications

Fruit and nut trees such as mango, pineapples, papaya, banana, guava, cashew, passion fruit, and avocado are important components of tropical ecosystems. They are sources of vitamins, minerals and carbohydrates and have great economic importance as sources of both household incomes and national revenues. Fruit and nut trees provide important adaptive values and tend to be more resilient to climate change due to their perennial nature, and they have the property of sequestering carbon. World production and trade of fresh tropical fruit are expected to expand over decades, with developing countries accounting for about 98% of total production, while developed …

Patient Predictors Of Poor Drug Sensitive Tuberculosis Treatment Outcomes In Kyiv Oblast, Ukraine, Omowunmi Aibana, Andrej Slavuckij, Mariya Bachmaha, Viatcheslav Krasiuk, Natasha Rybak, Timothy P Flanigan, Vasyl Petrenko, Megan B Murray

Faculty, Staff and Student Publications

Background: Ukraine has high rates of poor treatment outcomes among drug sensitive tuberculosis (DSTB) patients, while global treatment success rates for DSTB remain high.

We evaluated baseline patient factors as predictors of poor DSTB treatment outcomes.

Methods: We conducted a retrospective analysis of new drug sensitive pulmonary TB patients treated in Kyiv Oblast, Ukraine between November 2012 and October 2014. We defined good treatment outcomes as cure or completion and poor outcomes as death, default (lost to follow up) or treatment failure. We performed logistic regression analyses, using routine program data, to identify baseline patient factors associated with poor outcomes. …

Genetic Diversity And Population Structure Of The African Catfish, Clarias Gariepinus (Burchell, 1822) In Kenya: Implication For Conservation And Aquaculture, James E. Barasa, Sinebongo Mdyogolo, Romulus Abila, Johannes Paul Grobler, Robert A. Skilton, Hesmari Bindeman, Moses Ndotono Njahira, Emily J. Chemoiwa, Otto G. Dangasuk, Boaz Kaunda-Arara, Erik Verheyen

All Peer-Reviewed Publications

African catfish, Clarias gariepinus, is an important species in aquaculture and fisheries in Kenya. Mitochondrial D-loop control region was used to determine genetic variation and population structure in samples of C. gariepinus from 10 sites including five natural populations (Lakes Victoria (LVG), Kanyaboli (LKG), Turkana (LTA), Baringo (LBA) and Jipe (LJP), and five farms (Sangoro Aquaculture Center (SAN), Sagana Aquaculture Centre (SAG), University of Eldoret Fish Farm (UoE), Kibos Fish Farm (KIB), and Wakhungu Fish Farm (WKU)) in Kenya. Similarly, samples from eight localities (four natural populations: LVG/LKG, LTA, LBA, and four farmed: SAN, SAG, KIB, UoE) were genotyped using …

Dna Barcodes, Expanded Distribution, And Redescription Of Apanteles Hemara Nixon, 1965 (Hymenoptera, Braconidae, Microgastrinae), A Potential Biocontrol Species Against Amaranth Leaf-Webbers In Africa, Jose Fernandez-Triana, Melanie Beaudin, Kees Van Achterberg, Mawufe K. Agbodzavu, Stephen T.O. Othim, Faith W. Nyamu, Komi K.M. Fiaboe

All Peer-Reviewed Publications

The microgastrine parasitoid wasp Apanteles hemara Nixon, 1965, is currently being considered as a potential biocontrol agent of amaranth leaf-webber pests in Africa. To facilitate future research and identification of the species, we characterize it from an integrative taxonomy perspective by providing a comprehensive morphological redescription, extensive illustrations (including the first images of the holotype), DNA barcodes, wasp biology, host data (Choreutidae and Crambidae caterpillars), and updated geographical distribution of the species (including eight new country records). Despite a wide distribution across four major biogeographical regions (mostly within the Old World tropics), the species seems to be relatively uniform from …

Systematics And Biology Of Cotesia Typhae Sp. N. (Hymenoptera, Braconidae, Microgastrinae), A Potential Biological Control Agent Against The Noctuid Mediterranean Corn Borer, Sesamia Nonagrioides, Laure Kaiser, Jose Fernandez-Triana, Claire Capdevielle-Dulac, Célina Chantre, Matthieu Bodet, Ferial Kaoula, Romain Benoist, Paul André Calatayud, Stéphane Dupas, Elisabeth A. Herniou, Rémi Jeannette, Julius Obonyo, Jean François Silvain, Bruno Le Ru

All Peer-Reviewed Publications

Many parasitoid species are subjected to strong selective pressures from their host, and their adaptive response may result in the formation of genetically differentiated populations, called host races. When environmental factors and reproduction traits prevent gene flow, host races become distinct species. Such a process has recently been documented within the Cotesia flavipes species complex, all of which are larval parasitoids of moth species whose larvae are stem borers of Poales. A previous study on the African species C. sesamiae, incorporating molecular, ecological and biological data on various samples, showed that a particular population could be considered as a distinct …

Holistic Self-Care: Tai Chi, Qigong Training For Caregivers At An Alzheimer Disease Assisted Living Facility, Sunny Alperson, Christianne Fowler

Ellmer School of Nursing Faculty Publications

With the growing number of older persons with chronic illnesses, the demand for professionals who can provide complex care is increasing as well. However, evidence shows that health professionals’ burnout rate has been consistently increasing for the last 3 decades. In an effort to alleviate some of the issues associated with caregiver burnout, we conducted mind-body training of self-compassion for professional caregivers at an Alzheimer disease (AD) specialty center for 5 weeks. The intervention combined seven simple centering movements of tai chi and qigong in a context of mettā (loving kindness meditation) language mindfulness. Data showed significant improvements in caregiver …

Heme Oxygenase 1 Expression After Traumatic Brain Injury And Effect Of Pharmacological Manipulation On Functional Recovery., Nicholas H. Russell

Theses and Dissertations

Traumatic Brain Injury (TBI) is an increasingly diagnosed constellation of injuries derived from acute mechanical trauma to the brain. With the rise of advanced neuroimaging techniques recent focus has oriented primarily towards the mild-moderate range of TBI which previously was missed diagnostically. Characteristically, these advances have shown increasing areas of micro-hemorrhage in susceptible areas of the brain and to date there are no treatment modalities targeting micro-hemorrhages or their sequelae. This dissertation explores the effects of the resulting heme processing response in the days following injury with a particular focus on inducing early heme clearance from the parenchyma using a …

Implementing Integrated Care In Family Medicine: Description And Outcomes In An Underserved Population, Zach Radcliff

Theses and Dissertations

Introduction: Family physicians provide access to medical and behavioral healthcare for many underserved populations. Integrating behavioral health clinicians into primary care practices has been proposed as one of the most effective ways to increase access to necessary behavioral health services for many Americans. Integrated behavioral healthcare (IBHC) has begun to be implemented in family medicine practices but there is limited research examining the impact for patients and clinic staff. This study begins to fill this gap in the literature by examining the effects of implementing integrated behavioral healthcare in an urban family medicine clinic in a medically underserved area.

Objective: …

Modified Pamam Dendrimers In Tunable Drug-Delivery Systems: A Sustained-Release Dendrimer Hydrogel For Anti-Glaucoma Drugs And Surface-Engineered Macrophages As Nanoparticle Carriers For Targeted Anti-Cancer Therapy, Christopher A. Holden

Theses and Dissertations

Two specific drug-delivery applications were sought in this work using polyamidoamine (PAMAM) dendrimers. One drug-delivery system used a novel dendrimer hydrogel (DH) for sustained delivery of anti-glaucoma drugs. In this work, PAMAM G3.0 dendrimers were covalently bonded with poly(ethylene glycol) (PEG­₁₂₀₀₀) molecules which were subsequently acrylated, resulting in photocurable DH conjugates. For pharmacological studies, DH were loaded with a solution of intraocular pressure lowering drugs, brimonidine and timolol maleate, and were characterized for in vitro release and ex vivo transport and uptake. DH formulations were shown to increase the loading of drug molecules, increase transcorneal drug delivery, and …

Pnpase In C. Elegans: Mutagenic Analysis To Complement Knockdown Studies, Danielle K. Seibert

Theses and Dissertations

PNPase is a gene implicated as a potential target for cancer therapy; human mutations also present with deafness, myopathies, and neuropathies. In this study, C. elegans was used to investigate the effect of knocking out PNPase in a whole animal. C. elegans knockdown studies have reported an extended lifespan via an increase in ROS production. Further noted are larger mitochondria and an increase in fzo-1 expression. Knockout animals previously constructed using CRISPR/Cas9 were used for this study. We aimed to confirm these findings validating previous studies. It was discovered that PNPase knockout animals demonstrated a similar lifespan extension that was …

Antidepressant-Like Effects Of Amisulpride, Ketamine, And Their Enantiomers On Differential-Reinforcement-Of-Low-Rate (Drl) Operant Responding In Male C57/Bl/6 Mice, Doug Smith

Theses and Dissertations

Major depressive disorder (MDD) is a widespread psychiatric disorder that affects millions of people worldwide and is hypothesized to occur due to impairments in several neurotransmitter systems, including the monoaminergic and glutamatergic neurotransmitter systems. Antidepressant medications targeting multiple monoamine neurotransmitters have been shown to be effective for the treatment of depression. Racemic amisulpride is an atypical antipsychotic that has been used at low doses to treat dysthymia, a mild form of depression, and functions as an antagonist at DA_2/3, 5-HT_2B, and 5-HT₇ receptors. Recent preclinical studies have suggested that the S(+) isomer may be more …

Role Of Translation Initiation In Regulation Of Epithelial Junctions And Cell Motility, Fahda Fawaz Alsharief

Theses and Dissertations

The integrity and barrier properties of intestinal epithelium are determined by specialized adhesive structures known as intercellular junctions; composed of adherens junctions (AJs), tight junctions (TJs) and focal adhesions that mediate cell-cell and cell matrix interactions, respectively. These two types of epithelial cell adhesions regulate each other during disruption and restitution of the epithelial barrier. Inflammatory cytokines such as interferon gamma (IFNγ) and tumor necrosis factor alpha (TNFα) are elevated during intestinal inflammation. The most notable effects of IFNγ and TNFα on intestinal epithelial homeostasis involve disruption of apical junctions and attenuation of cell migration. Although molecular mechanisms underlying these …

The Identity Formation Of South Asians: A Phenomenological Study, Shabana Shaheen

Theses and Dissertations

This research explores the lived experiences of South Asians college students. This research, through a qualitative study that is rooted in the philosophy of phenomenology, explores the essence South Asians’ identity formation. Qualitative data was collected through semi-structured interviews with South Asian college students. The data analysis was under a phenomenological lens that centered the lived experiences and the essence of these experiences in the results. Seven themes emerged from this phenomenological study: negotiating bicultural identity, model minority expectations, meaningful impact of religious spaces, understandings of intra-community tensions, racialization of Islamophobia, understandings of South Asian identity and efficacy of Asian …

Investigation Of Anticoagulation Properties Of Sulfated Glycosaminoglycan Mimetics, Elsamani I. Abdelfadiel

Theses and Dissertations

Abstract

INVESTEGATION OF ANTICOAGULATION PROPERTIES OF SULFATED

GLYCOSAMINOGLYCAN MIMETICS

By Elsamani Ismail Abdelfadiel, MS

A thesis submitted in partial fulfillment of the requirements for the degree of Master of

Science at Virginia Commonwealth University

Virginia Commonwealth University, 2017.

Supervisor: Umesh R Desai

Professor, Department of Medicinal Chemistry

The existence of thrombosis in numerous pathophysiological situations formed a vast necessity for anticoagulation therapy. Thrombin and factor Xa are the only two factors of the entire coagulation cascade that have been major targets for regulation of clotting via the direct and indirect mechanism of inhibition. Our recent discovery of sulfated non-saccharide glycosaminoglycan …

Expanding Monoamine Transporters Pharmacology Using Calcium Channels, Iwona Ruchala

Theses and Dissertations

Research in drug development meets many challenges including lengthy, complex and costly procedures to identify novel pharmacotherapies. In our lab, we developed a method for fast screening of small molecules that interact with monoamine transports – dopamine and serotonin (DAT, SERT). These membrane proteins play important roles in brain neurotransmission responsible for cognition, motion and pleasure. Dysfunction in dopaminergic and serotonergic systems result in neurological disorders such as depression, Attention Deficit Hyperactivity Disorder (ADHD), schizophrenia and addiction.

DAT and SERT are responsible for uptake of dopamine (DA) or serotonin (5HT) into the synapse and they limit neurotransmitter signaling. Drugs that …

Drinking Motives Underlying Internalizing And Externalizing Pathways To Alcohol Misuse In College Students, Jeanne Savage

Theses and Dissertations

Alcohol misuse, including heavy episodic use and negative consequences, is a major public health concern and a particular problem among college students. The etiology of alcohol misuse is not well resolved, with multiple and often contradictory factors implicated in its development. Genetic factors influence alcohol misuse but few specific genes have been identified. A potential reason for these challenges is that alcohol misuse is phenotypically and genetically heterogeneous; that is, there are multiple causal pathways underlying its development. Previous typologies have suggested that distinct internalizing and externalizing pathways are involved, with corresponding differences in profiles of personality, temperament, and comorbid …

How Does Alcoholics Anonymous Affect Drinking Outcomes? A Grounded Theory Perspective, Bryan Boyles

Theses and Dissertations

The mutual aid of Alcoholics Anonymous helps more people with alcohol-related problems than all forms of clinical treatment combined. Yet, efforts to understand AA’s action have been conducted, almost exclusively, from a clinical perspective. Thus far, the literature representing Alcoholics Anonymous from the perspective of its members is very limited. This study uses qualitative content analysis of the AA literature and a grounded theory approach to AA’s fellowship to provide an insider’s perspective of change processes associated with AA involvement. This understanding of the AA literature posits that Alcoholics Anonymous involvement results in life improvement, changes in spirituality, improved well-being, …

Mast Cell Activation By Diverse Stimuli Can Be Suppressed By Steroid Therapy And Targeting The Fyn-Stat5b Cascade, Anuya Paranjape

Theses and Dissertations

Mast cells are critical effectors of allergic disease that can be activated by numerous stimuli. We have examined mast cell control by the inflammatory cytokine, IL-33, as well as IgG. In the first study reported here, we found that the synthetic glucocorticoid, dexamethasone, potently and rapidly suppressed IL-33-induced cytokine production from murine bone marrow–derived and peritoneal mast cells, as well as human mast cells. Dexamethasone also antagonized IL-33-mediated enhancement of IgE-induced cytokine production and migration. Although dexamethasone had no effect on IL-33-induced phosphorylation of MAP kinases or NFκB p65 subunit, it antagonized AP-1 and NFκB-mediated transcriptional activity. Finally, intraperitoneal administration …

Contribution Of Asc-Inflammasome To Vascular Endothelial Dysfunction: Role Of Rna Receptor Rig-I, Ashley Pitzer

Theses and Dissertations

Retinoic acid-inducible gene-I (RIG-I) is a putative RNA helicase and recently identified as a cytosolic RNA receptor in mammalian cells. The role of RIG-I in the regulation of vascular function under physiological and pathological conditions is unknown. Recent studies have shown that the inflammasome serves as a crucial initiator of cytokine-mediated inflammation mediating the pathogenesis of cardiovascular disease. The present study tested whether RIG-I activation triggers inflammasome formation and subsequent cytokine-mediated inflammation in the endothelium of mice coronary arteries. Using both genetic and pharmacological interventions of the RIG-I inflammasome, we first characterized whether specific activation of RIG-I via 3pRNA transfection …

A Hierarchical Architectural Framework For Securing Unmanned Aerial Systems, Matthew Leccadito

Theses and Dissertations

Unmanned Aerial Systems (UAS) are becoming more widely used in the new era of evolving technology; increasing performance while decreasing size, weight, and cost. A UAS equipped with a Flight Control System (FCS) that can be used to fly semi- or fully-autonomous is a prime example of a Cyber Physical and Safety Critical system. Current Cyber-Physical defenses against malicious attacks are structured around security standards for best practices involving the development of protocols and the digital software implementation. Thus far, few attempts have been made to embed security into the architecture of the system considering security as a holistic problem. …

Validation Study Of The Science Literacy Assessment: A Measure To Assess Middle School Students' Attitudes Toward Science And Ability To Think Scientifically, Tammy Mckeown

Theses and Dissertations

This study investigated validity evidence for the Science Literacy Assessment, an instrument designed to assess middle school students’ ability to think scientifically as well as their motivation and beliefs about science (Fives, Huebner, Birnbaum, & Nicolich, 2014). Specifically, three sources of evidence were considered; internal structure, concurrent criterion-related, and predictive criterion-related. Exploratory factor analysis was utilized to examine the underlying factor structure of each of the instrument’s two components, motivation and beliefs related to science and demonstrated scientific literacy. Pearson product-moment correlations were calculated to determine the relationship between scores on the motivation and belief component of the Science Literacy …

Predictors Of Vicarious Traumatization Among Trauma Clinicians And General Mental Health Providers: A Comparison, Shaina Gulin

Theses and Dissertations

Vicarious traumatization (VT) describes the gradual, transformative shifts in internal experience that occur as a result of cumulative exposure to clients’ trauma material. VT is thought to develop in the therapist due to empathic engagement with clients, resulting in profound disruptions in frame of reference. Because VT is conceptualized as a condition that develops due to frequent exposure to clients’ traumatic material, a rapidly emerging body of theoretical literature suggests that clinicians can safeguard against VT by maintaining a more balanced workload (i.e., a caseload of clients with a variety of presenting problems) and limiting the number of trauma cases. …

Misophonia: An Investigation Of The Lesser-Known Decreased Sound Tolerance Condition, Shannon Cusack

Theses and Dissertations

Misophonia is a decreased sound tolerance condition (DST) that is not yet well-established in the literature. However, the existing research on misophonia shows that it is occurring at substantial levels in the population. The majority of the existing literature has focused on the clinical correlates of misophonia. Although the correlates have been investigated, there is no accepted mechanism behind misophonia etiology or maintenance. The present study examined three hypotheses in order to start to identify potential mechanisms behind misophonia: how emotional predilections are related to the emotional response, how obsessive-compulsive disorder (OCD) symptoms and misophonia are related, and the possibility …

Understanding Over-The-Counter Medication Use And Decision-Making Among Community-Dwelling Us Older Adults: A Mixed-Methods Approach, Yoshita Paliwal

Theses and Dissertations

Introduction

Older adults are regular consumers of over-the-counter (OTC) medications. OTC medications are generally considered safe, and convenient to use without requiring a prescription. However, the safety of an OTC medication and the final health outcome depends in part upon consumers’ perceptions, beliefs, and their decision-making about OTC medication use. The main objectives of this study were: 1) to examine the prevalence and characteristics of OTC medication use among community-dwelling US older adults (65 years and older), 2) to explore older adults’ knowledge, attitudes, beliefs and experiences about OTC medications, and 3) to elicit the OTC medication decision-making process in …

Developmental Trajectories Of Alcohol Use And Alcohol Use Disorder, Elizabeth C. Long

Theses and Dissertations

Alcohol use (AU) and alcohol use disorder (AUD) are leading causes of morbidity, premature death, and economic burden. They are also associated with high levels of disability and many other negative outcomes. Twin and family studies have consistently shown that AU and AUD are complex traits influenced by both genetic and environmental factors. Although much has been learned about the genetic and environmental etiology of AU and AUD, significant gaps remain. These include the need for a more comprehensive understanding of the roles of risk and protective factors, and the nature of developmental trajectories underpinning the progression from AU to …

The Role Of Afrocentric Features In Mental Healthcare Utilization And Counselor Preferences In Black College Students, Randl B. Dent

Theses and Dissertations

Though mental health issues are prevalent in Black young adults, they underutilize mental healthcare services. This research examined the role of feature-based discrimination in mental healthcare (under)utilization. Study 1, a secondary analysis of the National Longitudinal Study of Adolescent to Adult Health, provided no evidence supporting a link between skin tone and mental healthcare utilization, when controlling for depression diagnosis. However, when controlling for depression symptoms, there was a trend such that Black young adults with darker, as opposed to lighter, skin tone utilized healthcare less. Study 2, an experimental study with 33 Black college students, showed 73% of the …

Ain’T I Black Enough?: Anxiety Related To The Questioning Of One’S Group Membership, Michelle Stith

Theses and Dissertations

During late adolescence and emerging adulthood, individuals undergo identity formation (Erikson, 1956). During this period, individuals may be insecure about their group memberships (Myers & DeWall, 2015). Subcategorizations have developed among Black people in America resulting from their traumatic history (Blair, Judd, Sadler, & Jenkins, 2002; Levingston & Brewer, 2002). This subcategorization creates in-group/out-group dichotomies. In order to increase one’s self-image, Social Identity Theory (Tajfel & Turner, 1979) poses that individuals attempt to enhance the image of their in-group. This study aimed to determine whether a person feels anxiety due to fear of rejection from the in-group when challenged on …

One In Eight: Deciding To Pursue A College-Going Possible Self In A High-Poverty High School, David B. Naff

Theses and Dissertations

There is considerable research evidence suggesting that low-income, racial minority students value education and aspire for postsecondary educational attainment (Bloom, 2007; Destin & Oyserman, 2009; Wigfield & Eccles, 2002). However, their performance in school often does not align with those values and ambitions, as these students tend to underachieve in comparison with their higher-income, non-minority peers (Reardon, 2011), with particular gaps found in those attending schools of concentrated poverty (Rowan, 2011). This gap between educational ambition and attainment suggests that the experience of living and going to school in a high-poverty context could be related to the motivational processes driving …

Pathways Linking Clinician Demographics To Mental Health Diagnostic Accuracy: An International Perspective, Julia Brechbiel

Theses and Dissertations

Significant research efforts have focused on examining the effect of patient factors on providing diagnoses across clinical settings; however, the influence of clinician demographics have received less attention. This study aimed to understand the impact of nonclinical factors such as clinician characteristics and response time on diagnostic accuracy. The study used data from a WHO field study of the ICD-11 development (n = 1822) that required clinicians to diagnose two case vignettes. Clinicians’ slower response times had a significant positive impact on their rates of diagnostic accuracy. However, there was no evidence that clinicians’ demographic features were directly related to …