Digital Commons Network^™

Quantitative Proteomic Analysis Of Stratum Corneum Dysfunction In Adult Chronic Atopic Dermatitis., Jason M Winget, Deborah Finlay, Kevin J Mills, Tom Huggins, Charles Bascom, Robert J Isfort, Robert L Moritz

Articles, Abstracts, and Reports

No abstract provided.

Snp-Based High Density Genetic Map And Mapping Of Btwd1 Dwarfing Gene In Barley, Xifeng Ren, Jibin Wang, Lipan Liu, Genlou Sun, Chengdao Li, Hong Luo, Dongfa Sun

Publications

A high-density linkage map is a valuable tool for functional genomics and breeding. A newly developed sequence-based marker technology, restriction site associated DNA (RAD) sequencing, has been proven to be powerful for the rapid discovery and genotyping of genome-wide single nucleotide polymorphism (SNP) markers and for the high-density genetic map construction. The objective of this research was to construct a high-density genetic map of barley using RAD sequencing. 1894 high-quality SNP markers were developed and mapped onto all seven chromosomes together with 68 SSR markers. These 1962 markers constituted a total genetic length of 1375.8 cM and an average of …

Daily Rhythms Of Physiological Parameters In The Dromedary Camel Under Natural And Laboratory Conditions, Ahmed A. Al-Haidary, Khalid A. Abdoun, Emad M. Samara, Aly B. Okab, Mamane Sani, Roberto Refinetti

Psychological Sciences Faculty Publications and Presentations

Camels are well adapted to hot arid environments and can contribute significantly to the economy of developing countries in arid regions of the world. Full understanding of the physiology of camels requires understanding of the internal temporal order of the body, as reflected in daily or circadian rhythms. In the current study, we investigated the daily rhythmicity of 20 physiological variables in camels exposed to natural oscillations of ambient temperature in a desert environment and compared the daily temporal courses of the variables. We also studied the rhythm of core body temperature under experimental conditions with constant ambient temperature in …

Reduced Motor Neuron Excitability Is An Important Contributor To Weakness In A Rat Model Of Sepsis, Paul Nardelli, Jacob A. Vincent, Randall K. Powers, Timothy C. Cope, Mark M. Rich

Neuroscience, Cell Biology & Physiology Faculty Publications

The mechanisms by which sepsis triggers intensive care unit acquired weakness (ICUAW) remain unclear. We previously identified difficulty with motor unit recruitment in patients as a novel contributor to ICUAW. To study the mechanism underlying poor recruitment of motor units we used the rat cecal ligation and puncture model of sepsis. We identified striking dysfunction of alpha motor neurons during repetitive firing. Firing was more erratic, and often intermittent. Our data raised the possibility that reduced excitability of motor neurons was a significant contributor to weakness induced by sepsis. In this study we quantified the contribution of reduced motor neuron …

Crispr Humans: Ethics At The Edge Of Science, Insoo Hyun

Center for the Study of Ethics in Society Papers

No abstract provided.

Mitigating Prevalence And Persistence Of L. Monocytogenes Using Novel Sanitation Strategies In Delis, Susan Rose Hammons

Open Access Dissertations

Ready-to-eat (RTE) deli meats sliced at retail are predicted to cause 83% of deli meat-associated listeriosis cases annually. While L. monocytogenes is commonly found in delis (55-90%), environmental prevalence varies by store (0-40%). Study 1 evaluated efficacy of 10 standardized SSOPs by testing 28 food and non-food contact surfaces for L. monocytogenes over 6 months in 30 retail delis. Intervention strategies employed in this study significantly decreased L. monocytogenesprevalence on FCS and NFCS in stores with moderate prevalence (0.5%, 0.8% reduction). However, we found that L. monocytogenes could persist despite implementation of SSOP control strategies. Study 2 assessed an …

Synthesis, Characterization, And Application Of Magnetic Gold Nanoclusters, Naveen Reddy Kadasala

Open Access Dissertations

Au–Fe3O4 nanocomposites are of great interest for technological reasons due to their combined optical and magnetic properties. Nanosized Fe3O4 particles are superparamagnetic and can also be coated with a layer of Au, which supports localized plasmon resonances for useful optical properties. The dual properties of Au–Fe3O 4 nanoparticles have inspired chemists to design a variety of synthetic routes, some with good control of overall shape and size. However, most approaches require organic solvents, surfactants, or harsh chemical conditions. In this thesis we describe a simple and highly reproducible synthesis of magnetically active gold nanoclusters (MGNCs) with average particle sizes under …

Mass Spectrometric Characterization Of Remotely Charged Amino Acids And Peptides, Damodar Koirala

Open Access Dissertations

Ion–molecule reactions in a flowing afterglow are used to examine the electronic structure of 3- and 4-pyridinylnitrene-n-oxide radical anions. Reactions with nitric oxide are generally similar to those reported previously for other aromatic nitrene radical anions. In particular, phenoxide formation by nitrogen–oxygen exchange is observed with both isomers. Oxygen atom abstraction by NO is also observed with both isomers. Very significant differences in the reactivity are observed in the reactions of the two isomers with carbon disulfide. The reactivity of the 3-noxide isomer with CS2 is similar to that observed previously for nitrene radical anions, and reactions of the n-oxide …

The Regulation Of Synthetic Nucleic Acids Under The Human Pathogens And Toxins Act

UBC Law Review

No abstract provided.

The Role Of Developmental Plasticity In Response To Salinity On The Evolution Of Alternative Reproductive Tactics In The Sailfin Molly, Poecilia Latipinna (Poeciliidae: Poecilia: Mollienesia), Kelly Elise Hogan

All Theses

Variability in life history and morphology across conspecific populations is a widespread phenomenon and may be attributed to life history trade-offs in response to environmental variation. Such trade-offs between growth, maintenance, and reproduction often occur via developmental plasticity, which allows organisms to shift their developmental trajectories to maximize fitness in a given environment. Sailfin mollies (Poecilia latipinna) exhibit interpopulation variation in life history and morphological traits, and inhabit springs, tidal creeks, and estuaries that range from freshwater to seawater. Male size at maturity is smaller on average in freshwater springs than in brackish water marshes where these fish are most …

Mitigating The Effects Of Test Anxiety Through A Relaxation Technique Called Sensory Activation, Marylynne Abbott

Dissertations

Test anxiety is a phenomenon which has been researched for decades. Student performance, goal attainment, and personal lives are all negatively affected by the multiple factors of test anxiety. This quantitative study was designed to determine if a particular relaxation technique, called sensory activation, could mitigate the symptoms and effects of test anxiety. The Test and Anxiety Examination Measure, developed by Brooks, Alshafei, and Taylor (2015), was used to measure test anxiety levels before and after implementation of the sensory activation relaxation technique. Two research questions guided the study using not only the overall test anxiety score from the Test …

Enhancing The Methane Yield Of A Landfill Leachate Bioreactor, Paul Vecchiarelli

All Theses

Our thriving modern society requires unprecedented amounts of natural resources to maintain pace with rapidly evolving standards of living in industrialized regions. Due to population growth and wide-scale urbanization, over 3.5x106 metric tons of solid wastes are produced daily around the world. In addition to land, water, and raw material strain generated by solid waste dumping, pristine resources are needed for food, energy, and potable water — instead being used through waste management. Reducing landfill leachate volume and toxicity while deriving value-added products from leachate is a sustainable alternative to traditional solid waste management. Carbon mass balances of biomass grown …

Haptic Feedback Effects On Human Control Of A Uav In A Remote Teleoperation Flight Task, Evan Joseph Sand

All Theses

The remote manual teleoperation of an unmanned aerial vehicle (UAV) by a human operator creates a human-in-the loop system that is of great concern. In a remote teleoperation task, a human pilot must make control decisions based upon sensory information provided by the governed system. Often, this information consists of limited visual feedback provided by onboard cameras that do not provide an operator with an accurate portrayal of their immediate surroundings compromising the safety of the mobile robot. Due to this shortfall, haptic force feedback is often provided to the human in an effort to increase their perceptual awareness of …

Gender Hybridity In H.G. Wells' The Island Of Dr. Moreau, Charlotte Rene Powell

All Theses

Critical scholarship regarding The Island of Dr. Moreau typically concerns themes of evolution and internal corruption. However, the unstudied question of evolution in The Island of Dr. Moreau remains the places where Edward Prendick’s performances of unconventional masculinity on the island inhabit language used to describe bestial regression and indicate a place where rhetoric regarding evolution and gender intersect. The gap in the critical conversation surrounding the representation of Prendick is an important place where the concept of hybridity and evolution can and should be extended. Through a close reading of Prendick’s reactions to events that occur over the course …

Development Of Dynamic Testing Methods Using Facial Expression Analysis To Evaluate Packaging Design In A Realistic Shopping Environment, Richard Blake Bowen

All Theses

80 to 95 percent of all new product launches fail (Dillon, 2011; Copernicus Marketing, Consulting, and Research, 2013). However, businesses can increase the chances of a successful product launch by better understanding consumer preferences and wants. Research done by McKinsey and Company shows that “more than 80 percent of top performers periodically tested and validated customer preferences during the development process, compared to 43 percent of bottom performers” (Gordon et al., 2010). With most purchasing decisions being made at the point of purchase, packaging is the last opportunity for businesses to influence the consumers decision to purchase their product. Packaging …

Phenotypic Evolution Of Middle American Tree Frogs: Allometric Effects, And Larval And Adult Evolutionary Independence, David Antonio SáNchez

Biology Dissertations - Archive

The shape and size of vertebrates is shaped by adaptive processes and phylogenetic relationships, and constrained by mechanical and developmental constraints. We quantified the diversity of the skull using geometric morphometrics, studied the shape of the oral disc of tadpoles, constructed and corrected phylogenies of the Middle American Tree frogs, a monophyletic lineage with highly diverse shapes and sizes distributed in Central and North America, and temperate Eurasia. Four major clades are identified in the Middle American Tree frogs. We measured allometric effects and assessed the independence between the evolutionary history of the larval and the adult phenotype in two …

How Do Oil, Gas, And Water Interact Near A Subsea Blowout?, Scott A. Socolofsky, E. Eric Adams, Claire B. Paris-Limouzy, Di Yang

C-IMAGE Publications

Oil and gas from a subsea blowout shatter into droplets and bubbles that rise through the water column, entraining ambient seawater and forming a plume. Local density stratification and currents eventually arrest this rising plume, and the entrained water, enriched with dissolved hydrocarbons and some of the smaller oil droplets, forms one or more subsurface intrusion layers. Beyond the plume and intrusion layer(s), droplets and bubbles advect and diffuse by local currents and dissolve and biodegrade as they rise to the surface, where they are transported by wind and waves. These processes occur over a wide range of length scales …

Methods Of Oil Detection In Response To The Deepwater Horizon Oil Spill, Helen K. White, Robyn N. Conmy, Ian R. Macdonald, Christopher M. Reddy

C-IMAGE Publications

Detecting oil in the northern Gulf of Mexico following the Deepwater Horizon oil spill presented unique challenges due to the spatial and temporal extent of the spill and the subsequent dilution of oil in the environment. Over time, physical, chemical, and biological processes altered the composition of the oil, further complicating its detection. Reservoir fluid, containing gas and oil, released from the Macondo well was detected in surface and subsurface environments. Oil monitoring during and after the spill required a variety of technologies, including nimble adaptation of techniques developed for non-oil-related applications. The oil detection technologies employed varied in sensitivity, …

Emotion Regulation During Threat: Parsing The Time Course And Consequences Of Safety Signal Processing, Kathryn R. Hefner, Edelyn Verona, John J. Curtin

Psychology Faculty Publications

Improved understanding of fear inhibition processes can inform the etiology and treatment of anxiety disorders. Safety signals can reduce fear to threat, but precise mechanisms remain unclear. Safety signals may acquire attentional salience and affective properties (e.g., relief) independent of the threat; alternatively, safety signals may only hold affective value in the presence of simultaneous threat. To clarify such mechanisms, an experimental paradigm assessed independent processing of threat and safety cues. Participants viewed a series of red and green words from two semantic categories. Shocks were administered following red words (cue+). No shocks followed green words (cue‐). Words from one …

Induction Of Human Embryonic Stem Cell Derived Retinal Stem Cells In Vitro Using Transient Overexpression Of Messenger Rna For Blimp, Onecut1 And Otx2, Helen Cifuentes

Dissertations, Masters Theses, Capstones, and Culminating Projects

No abstract available

Uab To Host New Summer Commencement Ceremony, Doctoral Hooding, Aug. 13, Shannon Thomason

Browse All News

No abstract provided.

Hydrologic Characteristics Of Summer Stream Temperatures In Little Creek And Scotts Creek At The Swanton Pacific Ranch, Justin M. Louen

Master's Theses

Stream temperature impacts have resulted in increased restrictions on land management such as timber harvest and have created considerable uncertainty for future planning and management of redwood forestlands. Challenges remain in the assessment of downstream cumulative effects given the complexity of stream temperature dynamics. The goal of this research is to identify the risk of downstream temperature heating based on the summer low flow residence times, stream morphological characteristics, stream water storage, and heat budget exchanges. Stream temperature, hydrologic, climatic, and channel morphological data were collected on two, approximately 800 m stream reaches on Little Creek and Scotts Creek located …

Georgia Southern Aquaponics: Sustainable Food Production On Campus, Subhrajit Saha, Amber Monroe

Sustainability Showcase (2014-2022)

Georgia Southern Aquaponics: Sustainable Food Production on Campus

Dr. Subhrajit Saha, Amber Monroe, Ryan M. Day, Dept. of Biology ($21,132)

Food is one of the key focal areas of campus sustainability initiatives and this project studied an aquaponics system and trained Georgia Southern University students on sustainable food production at their residences. Aquaponics is a sustainable and alternative form of agriculture, where aquatic fauna and crops are grown together in a mutually beneficial way. This project also produced four different types of crops, a leafy vegetable (Lettuce), a fruit-bearing vegetable (Tomato), a fruit (Strawberry) and an herb (Mint) with crayfish …

Vascular Endothelial Growth Factor-A Gene Electrotransfer Promotes Angiogenesis In A Porcine Model Of Cardiac Ischemia, Anna A. Bulysheva, Barbara Hargrave, Nina Burcus, Cathryn G. Lundberg, Len Murray, Richard Heller

Bioelectrics Publications

This study aimed to assess safety and therapeutic potential of gene electrotransfer (GET) as a method for delivery of plasmid encoding vascular endothelial growth factor A (VEGF-A) to ischemic myocardium in a porcine model. Myocardial ischemia was induced by surgically occluding the left anterior descending coronary artery in swine. GET following plasmid encoding VEGF-A injection was performed at four sites in the ischemic region. Control groups either received injections of the plasmid without electrotransfer or injections of the saline vehicle. Animals were monitored for 7 weeks and the hearts were evaluated for angiogenesis, myocardial infarct size and left ventricular contractility. …

Diabetes, Insulin Secretion, And Beta Cell Function, Jeffery Tessem

BYU Research Development Office Research Networking Conference

The intricate processes of nutrient metabolism and their biochemical pathways are fundamental to understanding human physiology and health. This research focuses on exploring the biochemical mechanisms underlying nutrient metabolism and how deviations in these processes can lead to metabolic disorders and associated human diseases.

Validation Of Reference Genes For Robust Qrt-Pcr Gene Expression Analysis In The Rice Blast Fungus Magnaporthe Oryzae, Sarena Che Omar, Michael A. Bentley, Giulia Morieri, Gail M. Preston, Sarah J. Gurr, Richard Wilson (Editor)

Department of Plant Pathology: Faculty Publications

The rice blast fungus causes significant annual harvest losses. It also serves as a genetically- tractable model to study fungal ingress. Whilst pathogenicity determinants have been unmasked and changes in global gene expression described, we know little about Magnaporthe oryzae cell wall remodelling. Our interests, in wall remodelling genes expressed during infection, vegetative growth and under exogenous wall stress, demand robust choice of reference genes for quantitative Real Time-PCR (qRT-PCR) data normalisation. We describe the expression stability of nine candidate reference genes profiled by qRT-PCR with cDNAs derived during asexual germling development, from sexual stage perithecia and from vegetative mycelium …

Perceived Emotional Invalidation In A Developmental Context: Does Gender Matter?, Brian B. Johnson

USC Aiken Psychology Theses

Emotional invalidation is the dismissal, minimization, or punishment of an individual’s emotional experience (Linehan, 1993). Although it has been sparsely studied, the research that has been conducted indicates that it is likely implicated in a multitude of psychopathology and adjustment issues. The current study had three main objectives. The first of these was to investigate the current perceptions of emotional invalidation in a peer interaction for emerging adults and how that is predicted by gender, perceptions of childhood emotional invalidation via caregivers, and gender of the caregivers. The second objective of this study was to investigate the propagation of emotionally …

Chemical Composition Of Macondo And Other Crude Oils And Compositional Alterations During Oil Spills, Edward B. Overton, Terry L. Wade, Jagoš R. Radović, Buffy M. Meyer, M. Scott Miles, Stephen R. Larter

C-IMAGE Publications

Crude oils are some of the most complex and diverse organic mixtures found in nature. They contain thousands of different compounds belonging to several compound classes, with the main ones being hydrocarbons and their heteroatom (N, S, and O)-containing analogs, called non-hydrocarbons. In general, all crude oils contain the same types of chemical structures, but these compounds can be in highly variable proportions in crude oils drawn from different reservoir conditions and locations. Both the types of compounds and their respective quantities change rapidly once the crude oil is spilled into the environment, making the circumstances associated with every spill …

Weathering Of Oil Spilled In The Marine Environment, Matthew A. Tarr, Phoebe Zito, Edward B. Overton, Gregory M. Olson, Puspa L. Adhikari, Christopher M. Reddy

C-IMAGE Publications

Crude oil is a complex mixture of many thousands of mostly hydrocarbon and nitrogen-, sulfur-, and oxygen-containing compounds with molecular weights ranging from below 70 Da to well over 2,000 Da. When this complex mixture enters the environment from spills, ruptures, blowouts, or seeps, it undergoes a continuous series of compositional changes that result from a process known as weathering. Spills of petroleum involving human activity generally result in more rapid input of crude oil or refined products (diesel, gasoline, heavy fuel oil, and diluted bitumens) to the marine system than do natural processes and urban runoffs. The primary physicochemical …

Elevated Integrin Α6Β4 Expression Is Associated With Venous Invasion And Decreased Overall Survival In Non-Small Cell Lung Cancer, Rachel L. Stewart, Dava West, Chi Wang, Heidi L. Weiss, Tamas S. Gal, Eric B. Durbin, William O'Connor, Min Chen, Kathleen L. O'Connor

Pathology and Laboratory Medicine Faculty Publications

Lung cancer carries a poor prognosis and is the most common cause of cancer-related death worldwide. The integrin α6β4, a laminin receptor, promotes carcinoma progression in part by cooperating with various growth factor receptors to facilitate invasion and metastasis. In carcinoma cells with mutant TP53, the integrin α6β4 promotes cell survival. TP53 mutations and integrin α6β4 overexpression co-occur in many aggressive malignancies. Because of the high frequency of TP53 mutations in lung squamous cell carcinoma (SCC), we sought to investigate the association of integrin β4 expression with clinicopathologic features and survival in non–small cell lung cancer (NSCLC). We constructed …