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Alcohol Use Variability In A Community-Based Sample Of Nonstudent Emerging Adult Heavy Drinkers, Cathy Lau-Barraco, Abbly L. Braitman, Amy L. Stamates, Ashley N. Linden-Carmichael

Psychology Faculty Publications

BACKGROUND: While nonstudent emerging adults are at elevated risk for experiencing alcohol-related problems, there remains a paucity of research devoted specifically to addressing drinking in this group.

OBJECTIVES: The present study sought to offer unique insights into nonstudent drinking by examining drinking variability across 30 days using a retrospective diary method. Specific aims were to: (1) compare within- and between-person variability in alcohol use across 30 days, and (2) determine the extent to which central social-cognitive between-person factors (i.e., social expectancies, perceived drinking norms, social drinking motivations) predict between-person alcohol use as well as within-person variability in drinking.

METHODS: Participants …

Potent And Broad Inhibition Of Hiv-1 By A Peptide From The Gp41 Heptad Repeat-2 Domain Conjugated To The Cxcr4 Amino Terminus., George J. Leslie, Jianbin Wang, Max W. Richardson, Beth S. Haggarty, Kevin L. Hua, Jennifer Duong, Anthony J. Secreto, Andrea P.O. Jordon, Josephine Romano, Kritika E. Kumar, Joshua J. Declercq, Philip D. Gregory, Carl H. June, Michael J. Root, James L. Riley, Michael C. Holmes, James A. Hoxie

Department of Biochemistry and Molecular Biology Faculty Papers

HIV-1 entry can be inhibited by soluble peptides from the gp41 heptad repeat-2 (HR2) domain that interfere with formation of the 6-helix bundle during fusion. Inhibition has also been seen when these peptides are conjugated to anchoring molecules and over-expressed on the cell surface. We hypothesized that potent anti-HIV activity could be achieved if a 34 amino acid peptide from HR2 (C34) were brought to the site of virus-cell interactions by conjugation to the amino termini of HIV-1 coreceptors CCR5 or CXCR4. C34-conjugated coreceptors were expressed on the surface of T cell lines and primary CD4 T cells, retained the …

Microplastic In Surface Waters Of Urban Rivers: Concentration, Sources, And Associated Bacterial Assemblages, Amanda Rae Mccormick, Timothy Hoellein, Maxwell G. London, Joshua Hittie, John J. Kelly, John W. Scott

Biology: Faculty Publications and Other Works

The ecological dynamics of microplastic (<5 mm) are well documented in marine ecosystems, but the sources, abundance, and ecological role of microplastic in rivers are unknown and likely to be substantial. Microplastic fibers (e.g., synthetic fabrics) and pellets (e.g., abrasives in personal care products) are abundant in wastewater treatment plant (WWTP) effluent, and can serve as a point source of microplastic in rivers. The buoyancy, hydrophobic surface, and long transport distance of microplastic make it a novel substrate for the selection and dispersal of unique microbial assemblages. We measured microplastic concentration and bacterial assemblage composition on microplastic and natural surfaces upstream and downstream of WWTP effluent sites at nine rivers in Illinois, United States. Microplastic concentration was higher downstream of WWTP effluent outfall sites in all but two rivers. Pellets, fibers, and fragments were the dominant microplastic types, and polymers were identified as polypropylene, polyethylene, and polystyrene. Mean microplastic flux was 1,338,757 pieces per day, although the flux was highly variable among nine sites (min = 15,520 per day, max = 4,721,709 per day). High-throughput sequencing of 16S rRNA genes showed bacterial assemblage composition was significantly different among microplastic, seston, and water column substrates. Microplastic bacterial assemblages had lower taxon richness, diversity, and evenness than those on other substrates, and microplastic selected for taxa that may degrade plastic polymers (e.g., Pseudomonas) and those representing common human intestinal pathogens (e.g., Arcobacter). Effluent from WWTPs in rivers is an important component of the global plastic “life cycle,” and microplastic serves as a novel substrate that selects and transports distinct bacterial assemblages in urban rivers. Rates of microplastic deposition, consumption by stream biota, and the metabolic capacity of microplastic biofilms in rivers are unknown and merit further research.

Draft Genome Sequence For A Urinary Isolate Of Nosocomiicoccus Ampullae, Catherine Putonti, Evann E. Hilt, Travis K. Price, Katherine Diebel, Alan J. Wolfe

Biology: Faculty Publications and Other Works

A draft genome sequence for a urinary isolate of Nosocomiicoccus ampullae (UMB0853) was investigated. The size of the genome was 1,578,043 bp, with an observed G+C content of 36.1%. Annotation revealed 10 rRNA sequences, 40 tRNA genes, and 1,532 protein-coding sequences. Genome coverage was 727× and consisted of 32 contigs, with an N₅₀ of 109,831 bp.

Body Image Perception And Internalization Problems Indicators In Mexican Adolescents, Cecilia Colunga-Rodríguez, Mercedes Gabriela Orozco-Solis, María Elena Flores-Villavicencio, José María De-La-Roca-Chiapas, Ricardo Gómez-Martínez, Alfonso Mercado, Julio César Vázquez-Colunga, Juan Carlos Barrera-De-León, Claudia Liliana Vázquez-Juárez, Mario Ángel-González

Psychological Science Faculty Publications

The aim of the study was to determine the existing differences by gender in the indicators of internalizing problems regarding the body image perception of Mexican adolescents. A descriptive and transversal study was developed. The participants were students from four public middle schools in Guadalajara Mexico. The instrument used was an online survey constituted by a sociodemographic section, an internalizing problems indicators scale (α = .85) and a body image perception scale (α = .70). Parental consent was obtained using a waiver of active consent. The survey was applied online, during school hours at the computer labs. Descriptive statistics were …

Conformational States Of Cytochrome P450 Oxidoreductase Evaluated By Förster Resonance Energy Transfer Using Ultrafast Transient Absorption Spectroscopy, Elizaveta A. Kovrigina, Brian Pattengale, Chuanwu Xia, Azamat R. Galiakhmetov, Jier Huang, Jung Ja Kim, Evgenii L. Kovrigin

Chemistry Faculty Research and Publications

NADPH-cytochrome P450 oxidoreductase (CYPOR) was shown to undergo large conformational rearrangements in its functional cycle. Using a new Förster resonance energy transfer (FRET) approach based on femtosecond transient absorption spectroscopy (TA), we determined the donor–acceptor distance distribution in the reduced and oxidized states of CYPOR. The unmatched time resolution of TA allowed the quantitative assessment of the donor–acceptor FRET, indicating that CYPOR assumes a closed conformation in both reduced and oxidized states in the absence of the redox partner. The described ultrafast TA measurements of FRET with readily available red–infrared fluorescent labels open new opportunities for structural studies in chromophore-rich …

The Role Of Acidosis In Fatigue: Pro Perspective, Robert H. Fitts

Biological Sciences Faculty Research and Publications

No abstract provided.

The Potential Antioxidant And Anti-Inflammatory Effects Of Date Seed Powder In Rats, Fatima Theyab Saif Amer Ai Meqbaali

Theses

Chronic diseases are among the main leading causes of mortality and morbidity in the United Arab Emirates (UAE). Oxidative stress (defined as an imbalance in the reactive oxygen species production/degradation ratio) in interaction with inflammation, has been highlighted as one major underlying mechanism in the development of these diseases. Due to their antioxidant and anti-inflammatory properties, polyphenols could prevent the development of chronic diseases. Interestingly, date seeds are particularly rich in such compounds. Nonetheless, an in vivo study aiming at studying and understanding antioxidant and anti-inflammatory effects of date seeds in serum and various organs is missing. Therefore, the present …

The Biological Function Of Hammerhead Rana Ribozyme In Human Genome, Ibtehal M. A. Awad

Theses

Hammerhead RNA [HHR] sequence originally found in viroids is also detected in many species. HHR are often found in introns of different genes ranging from prokaryotes to

eukaryotes. HHR can form specific tertiary structure that make specific region susceptible to cleavage. However, the role of the HHR is not clear in other life forms. This study aims to

Investigate the role of HHR in the intron 6 of (RECK) gene. Our study shows that when blocking the HHR sequence in the intron6, the production of RECK protein declines which may be due to the effect of HHR in splicing and …

Fabrication And Characterization Of Graphite Oxide Based Field Effect Transistors For Non­ Enzymatic Glucose-Sensor Application, Khadija Said Rahman

Theses

Graphite-oxide based metal–oxide–semiconductor field-effect transistors (MOSFETs) were fabricated and used as glucose sensor. Herein, graphite-oxide was assembled between two planer electrical electrodes. The sensitivity of the sensor has been enhanced by adding copper (Cu) or silver (Ag) nanoparticles. The nanoparticles were produced by sputtering and inert gas condensation inside an ultra-high vacuum compatible system, and they were self-assembled on the graphite-oxide. The sensitivity of the sensor was increased by an order of magnitude when the silver nanoparticles were added. The sensitivity of each MOSFET was studied at different concentrations of non-enzymatic glucose for potential use in medical and industrial applications.

The Prevalence Of Streptococcus Pyogenes And Its Emm Gene Types Among School Children In Ai Ain, Uae, Fatima Hammad Ali Al Shamisi

Theses

Streptococcus pyogenes, also known as Group a streptococcus (GAS), causes a wide variety of diseases in children and adults, and it frequently infects the pharynx and skin; pharyngitis and tonsillitis are one of the commonest diseases caused by this pathogen. GAS evaded by many virulence factors, however, the most significant part is the M protein, which plays a major role in its pathogenicity. Over 225 M protein types have been identified so far by the emm typing technique. Identification and emm typing of GAS is an essential part of epidemiological and pathogenic studies of streptococcal diseases, as different regions of …

Microarray Analysis Of Arabidopsis Wrky33 Mutants In Response To The Necrotrophic Fungus Botrytis Cinerea, Shamma Saeed Salem Al Shamsi

Theses

Plants are exposed to many environmental stresses that affect their growth and development. These stresses include biotic stresses (via organisms) and abiotic stresses (via environment). Plants respond to these stresses by transcriptional reprogramming and different signaling pathways. Arabidopsis thaliana has shown great sensitivity to the biotic stress Botrytis cinerea. The WRKY33 gene plays an important role in plant defense mechanism against this pathogen. The overall goal is to identify common regulated genes of wrky33 mutant and 35S:WRKY33 over expressing transgenic lines in response to B. cinerea; ultimately to improve plant stress tolerance in Arabidopsis. The specific aims are to: (1) …

Design, Fabrication And Characterization Of Low­ Power Gas Sensors Based On Organic-Inorganic Nano-Composition, Ayah Faeik Salim Abu Hani

Theses

Lower power consumption and higher performance are compelling demands in the electronic systems motivating the search for new materials, which are capable of fulfilling these demands. Recently, nanomaterial-based sensor technology has drawn considerable attention towards the development of sensing applications such as H2S gas sensors. Development of H2S gas sensors is crucial due to its extreme toxicity and damaging effects on both health and environment. This thesis aims to develop H2S gas sensors with enhanced sensitivity, flexibility, lower operating temperature, and high selectivity.

The proposed sensors present a contribution to the field of nano-electronics. They are designed based on the …

Effect Of Genipin On Cisplatin- Induced Nephrotoxicity, Eglal Orner Mahgoub

Theses

Cisplatin (CSP) is a potent and widely used chemotherapeutic agent. However, clinical efficacy of CSP is compromised due to the elicitation of nephrotoxicity and ototoxicity. In this study, I have investigated the nephroprotective effects of a phytochemical – genipin (GP) isolated from gardenia flower (Gardenia jasminoides), on a murine model of cisplatin-induced nephropathy. CSP-induced renal tissue injury was characterized by elevated levels of serum blood urea nitrogen (BUN), creatinine (Cr) and cystatin-C. In addition, levels of kidney injury molecule-1 (KIM-1) were increased in the renal tissues of CSP administered animals. CSP also induced renal oxidative stress, evidenced by increased NADPH …

Multiplex Social Ecological Network Analysis Reveals How Social Changes Affect Community Robustness More Than Resource Depletion, Jacopo A. Baggio, Shauna B. Burnsilver, Alex Arenas, James S. Magdanz, Gary P. Kofinas, Manlio De Domenico

Environment and Society Faculty Publications

Network analysis provides a powerful tool to analyze complex influences of social and ecological structures on community and household dynamics. Most network studies of social–ecological systems use simple, undirected, unweighted networks. We analyze multiplex, directed, and weighted networks of subsistence food flows collected in three small indigenous communities in Arctic Alaska potentially facing substantial economic and ecological changes. Our analysis of plausible future scenarios suggests that changes to social relations and key households have greater effects on community robustness than changes to specific wild food resources.

A New Method To Assess Long‐Term Sea‐Bottom Vertical Displacement In Shallow Water Using A Bottom Pressure Sensor: Application To Campi Flegrei, Southern Italy, Francesco Chierici, Giovanni Iannaccone, Luca Pignagnoli, Sergio Guardato, Marina Locritani, Davide Embriaco, Gian P. Donnarumma, Mel Rodgers, Rocco Malservisi, Laura Beranzoli

School of Geosciences Faculty and Staff Publications

We present a new methodology using bottom pressure recorder (BPR) measurements in conjunction with sea level, water column, and barometric data to assess the long‐term vertical seafloor deformation to a few centimeters accuracy in shallow water environments. The method helps to remove the apparent vertical displacement on the order of tens of centimeters caused by the BPR instrumental drift and by seawater density variations. We have applied the method to the data acquired in 2011 by a BPR deployed at 96 m depth in the marine sector of the Campi Flegrei Caldera, during a seafloor uplift episode of a few …

Classification Of Historical Vegetation In Oregon And Washington, As Recorded By General Land Office Sureyors, John Christy, Edward R. Alverson, Molly P. Dougherty, Susan C. Kolar, Clifford W. Alton, Susan M. Hawes, Gene Hickman, Jennifer A. Hiebler, Eric M. Nielsen

Institute for Natural Resources Publications

This classification of historical vegetation map units is based on General Land Office (GLO) surveyors' descriptions of vegetation, independent of modern vegetation classification. Vegetation data in the survey notes include species encountered along the survey lines, distances from section or quarter corners to "witness" or bearing trees, diameters of witness trees, and general descriptions of vegetation along each line. The surveyors were usually consistent in differentiating stands with dissimilar structure or composition, and they routinely segregated forest (“timber”), savanna ("openings"), and woodland ("scattering timber") from prairie. Today, these structural classes are often segregated by percent canopy cover or stand density, …

Ambivalence: Prerequisite For Success In Motivational Interviewing With Adolescents?, Sarah W. Feldstein Ewing, Timothy R. Apodaca, Jacques Gaume

Manuscripts, Articles, Book Chapters and Other Papers

BACKGROUND AND AIMS: The exploration and resolution of ambivalence play an essential role in motivational interviewing (MI) theory. However, most adolescent MI studies have not examined ambivalence as a contributor to behaviour change. This paper reviewed research findings on the role of ambivalence in the adolescent change process.

METHODS AND RESULTS: We undertook a narrative review of the published empirical and theoretical literature on ambivalence and mechanisms of change in MI for adolescents and found that current MI evaluations appear not to have access to reliable and valid measures of ambivalence in adolescence or neuroimaging methods to evaluate the mechanisms …

Hidden Markov Chain Analysis: Impact Of Misclassification On Effect Of Covariates In Disease Progression And Regression, Haritha Polisetti

USF Tampa Graduate Theses and Dissertations

Most of the chronic diseases have a well-known natural staging system through which the disease progression is interpreted. It is well established that the transition rates from one stage of disease to other stage can be modeled by multi state Markov models. But, it is also well known that the screening systems used to diagnose disease states may subject to error some times. In this study, a simulation study is conducted to illustrate the importance of addressing for misclassification in multi-state Markov models by evaluating and comparing the estimates for the disease progression Markov model with misclassification opposed to disease …

Clinical Outcomes Of Pneumocystis Pneumonia From A Tertiary Care Centre In Pakistan., Ali Bin Sarwar Zubairi, Hira Shahzad, Afia Zafar

Department of Medicine

Objective: To assess the predisposing immunocompromised states, administration of pneumocystis jirovecii pneumonia prophylaxis, the disease course and outcomes of patients with pneumocystis jirovecii pneumonia.

Methods: The retrospective study was conducted at the Aga Khan University Hospital in Karachi. The medical records of patients diagnosed with pneumocystis jirovecii pneumonia from January 1995 to October 2015 were retrieved. Baseline characteristics, clinical course, treatment, and mortality rates were noted. SPSS 19 was used for data analysis.

Results: Of the 37 patients, 24(64.9%) were men and 13(35.1%) were women. The overall mean presenting age was 47.08±16.21 years(range: 19-83 years). Ten (27%) patients were positive …

An Examination Of Information Literacy: A Survey Of Community College Faculty, Jamie D. Smith

Education Doctoral

The purpose of this study is to identify the information literacy dispositions that community college faculty find important to their disciplines and therefore, to their students. The study examined who community college faculty members believe is responsible for teaching various information literacy concepts. The study analyzed community college faculty responses related to information literacy skills. Research was conducted in accordance with the Association of College and Research Libraries’ information literacy framework and measured the importance of specific information literacy skills from the perception of faculty. A cross sectional design used quantitative survey methods modeled after Gullikson’s significant research on faculty …

The Trpm7 Interactome Defines A Cytoskeletal Complex Linked To Neuroblastoma Progression, J Middelbeek, K Vrenken, D Visser, E Lasonder, Et Al

School of Biomedical Sciences

Neuroblastoma is the second-most common solid tumor in children and originates from poorly differentiated neural crest-derived progenitors. Although most advanced stage metastatic neuroblastoma patients initially respond to treatment, a therapy resistant pool of poorly differentiated cells frequently arises, leading to refractory disease. A lack of insight into the molecular mechanisms that underlie neuroblastoma progression hampers the development of effective new therapies for these patients. Normal neural crest development and maturation is guided by physical interactions between the cell and its surroundings, in addition to soluble factors such as growth factors. This mechanical crosstalk is mediated by actin-based adhesion structures and …

Genetic And Biochemical Analysis Of The Micrococcin Biosynthetic Pathway, Philip Ross Bennallack

Theses and Dissertations

Declining antibiotic discovery and flourishing antibiotic resistance have led to a modern antibiotic crisis which threatens to compromise our ability to treat infectious disease. Consequently, there is significant interest in developing new antibiotics with novel modes of action and chemical properties. Ribosomally synthesized and post-translationally modified peptides (RiPPs) are natural compounds with the appealing attributes of being derived directly from a genetic template while possessing numerous exotic chemical features that contribute to stability and antimicrobial activity. Abundant in nature, their diverse range of biological activities makes them excellent prospects for antibiotic development. Thiopeptides, a RiPP family rich in chemical complexity, …

Currents In Fishery Management, Genevieve Depuy

University Honors Theses

This thesis provides an overview of fishery management policy types and attempts to determine best practices for biological sustainability in terms of species preservation and biomass recovery. Key concepts include biological sustainability, regulatory forces, and fishery management and output trends. The research question investigated is: what are the best policy practices for regulating marine fishery health and biological stability? The research process of this paper included a review of prior literature in the field of marine policy and fishery management, as well as the use of United States data from the Pacific and Atlantic coasts to evaluate management strategies. Based …

Nesting Microhabitat Use By A Ground-Nesting Songbird: Strict Preferences Or Random Choice?, Jazzmine Allen

University Honors Theses

Nest site choice of birds has the potential to affect predation rates on nests and reproductive success for many bird species, and is thus tied closely to fitness. Vegetation at a particular site influences concealment (and predation rate as a result). Research has shown that birds of various species base nest site choice on variables like nest height, and especially vegetation cover within the microhabitat around a site. I studied nest site choice in ground-nesting birds, which tend to experience high predation rates near the nest site, and therefore might be expected to be particular choosy about vegetation near the …

Acoustic Evidence Of Bats Using Rock Crevices In Winter: A Call For More Research On Winter Roosts In North America, Cliff A. Lemen, Patricia W. Freeman, Jeremy A. White

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

The Northern Long-eared Myotis (Myotis septentrionalis) is known to hibernate in mines and caves, often using cracks within these hibernacula as roost sites. We hypothesized that M. septentrionalis might use deep cracks in rock outcrops for hibernation as well. To test this hypothesis, we placed acoustical bat detectors near rock outcrops away from any known mines or caves during winter in Nebraska. We documented calls of M. septentrionalis as well as Perimyotis subflavus and Eptesicus fuscus in December near rock outcrops, which suggests that individuals of all three species were hibernating in rock crevices in winter. Of the …

E-Cigarette Use Among Women Of Reproductive Age: Impulsivity, Cigarette Smoking Status, And Other Risk Factors., Laura L. Chivers, Dennis J. Hand, Jeff S. Priest, Stephen T. Higgins

Department of Pediatrics Faculty Papers

INTRODUCTION: The study aim was to examine impulsivity and other risk factors for e-cigarette use among women of reproductive age comparing current daily cigarette smokers to never cigarette smokers. Women of reproductive age are of special interest because of the additional risk that tobacco and nicotine use represents should they become pregnant.

METHOD: Survey data were collected anonymously online using Amazon Mechanical Turk in 2014. Participants were 800 women ages 24-44years from the US. Half (n=400) reported current, daily smoking and half (n=400) reported smokingsociodemographics, tobacco/nicotine use, and impulsivity (i.e., delay discounting & Barratt Impulsiveness Scale). Predictors of smoking and …

Ultra-Filtration Of Human Serum For Improved Quantitative Analysis Of Low Molecular Weight Biomarkers Using Atr-Ir Spectroscopy, Franck Bonnier, Helene Blasco, Clement Wasselet, Guillaume Brachet, Renaud Respaud, Luis Felipe Cs. Carvalho, Dominque Bertrand, Mathew J. Baker, Hugh Byrne, Igor Chourpa

Articles

Infrared spectroscopy is a reliable, rapid and cost effective characterisation technique, delivering a molecular finger print of the sample. It is expected that its sensitivity would enable detection of small chemical variations in biological samples associated with disease. ATR-IR is particularly suitable for liquid sample analysis and, although air drying is commonly performed before data collection, just a drop of human serum is enough for screening and early diagnosis. However, the dynamic range of constituent biochemical concentrations in the serum composition remains a limiting factor to the reliability of the technique. Using glucose as a model spike in human serum, …

A Robust Speaker Identification Algorithm Based On Atomic Decomposition And Sparse Redundant Dictionary Learning, Thomas James Bryan Jr.

Theses and Dissertations

Speaker identification is performed in high additive white Gaussian noise environments by processing sounds as images in the time-frequency plane. The technique creates audio streams that remove background noise from speech while preserving speech quality. Atomic decomposition is implemented by the matching pursuit algorithm using Gabor or gammatone atomic dictionaries. Gabor atoms were originally proposed as fundamental building blocks of speech; whereas, gammatone atoms closely resemble the human cochlear impulse response. Atomic decomposition creates a sparsely populated time-frequency vector referred to as weight space. The populated positions in weight space have amplitude weights that are proportional to localized energy in …

The Effects Of Elevated Pco₂ And Temperature On The Embryonic Development, Larval Survivorship, Condition, Calcification, Morphology, And Behavior Of The Florida Stone Crab, Menippe Mercenaria, Philip Michael Gravinese

Theses and Dissertations

The emission of greenhouse gases into the atmosphere over the last century has increased atmospheric and oceanic temperatures, and has led to a decrease in oceanic pH. The increased ocean temperatures and reduced pH have been detrimental to marine life. In addition, Florida’s watersheds have suffered from decades of disrupted hydrology and diverted runoff, which has increased nutrients and changed coastal carbonate chemistry. Although the adult stages of many marine species are capable of tolerating fluctuations in environmental conditions (i.e., elevated pCO₂ tolerances to such conditions improve. and temperature), marine larvae that hatch in coastal habitats may not have the …