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Obesity And Asthma: Adiponectin Receptor 1 (Adipo R1) And Adiponectin Receptor 2 (Adipo R2) Are Expressed By Normal Human Bronchial Epithelial (Nhbe) Cells At Air-Liquid Interface (Ali) And Expression Changes With Il-13 Stimulation, Jennifer L. Bradley

Theses and Dissertations

Obesity is recognized as an important risk factor for the development of many chronic diseases such as hypertension, Type 2 diabetes mellitus (T2DM) cardiovascular disease, cancer, renal disease, neurologic dysfunction, metabolic syndrome and asthma (3, 4). Circulating serum adiponectin levels in obese asthmatics have been reported to be low. Therefore, we aimed to investigate the role of adiponectin in a mucus hypersecretion model and hypothesized that adiponectin would decrease IL-13 induced MUC5AC expression from differentiated NHBE cells and that increasing concentrations of IL-13 would cause a decrease in Adipo R1 and Adipo R2 expression. MUC5AC expression with exposure to adiponectin …

Exchanges Of Identity In Prehistoric Anatolian Figurines, Ellen H. Belcher, Karina Croucher

Publications and Research

This paper examines exchange imagery and ideology of the human body manifested through figurines from prehistoric (7 th –6 th millennia BC) Anatolian contexts. These figurines document local, regional and inter-regional communication of identity, use of materials, ideologies and skills. Taking a new approach to understanding the assemblages, this paper suggests four key themes of analysis: materials and materiality; fractured bodies; gender spectrum; and ambiguities and relationships.

Anthropomorphic Denial Of Fish Pain, Lynne U. Sneddon, Matthew C. Leach

Animal Sentience

Key (2016) affirms that we do not know how the fish brain processes pain but denies — because fish lack a human-like cortex — that fish can feel pain. He affirms that birds, like fish, have a singly-laminated cortex and that the structure of the bird brain is quite different from that of the human brain, yet he does not deny that birds can feel pain. In this commentary we describe how Key cites studies that substantiate mammalian pain but discounts the same kind of data as evidence of fish pain. We suggest that Key's interpretations are illogical, do not …

In Pursuit Of Women Scientists: Using Science Plays To Promote Women Entering Stem Disciplines, Danielle Hartman

Theses and Dissertations

Higher education currently seeks to increase female enrollment in STEM. Women face many challenges attempting to breach this male dominated arena with misconceptions, gender stereotypes, and few female role models. With the recent trend in higher education to encourage more women to enter the STEM disciplines and K-12 schools cutting funding for arts programs, theatre may be losing its value in the education system. The value of interdisciplinary studies is beginning to be forgotten during the grade school years as school boards battle budget cuts, but we can remind society of it through science plays. Theatre artists use other disciplines …

A Study Of The Action Of Risperidone At 5-Ht2a Receptors, Supriya A. Gaitonde

Theses and Dissertations

Risperidone is an ‘atypical’ antipsychotic and is approved by the USFDA mainly for the treatment of schizophrenia and symptoms of bipolar disorder. Risperidone (an SDA or serotonin-dopamine antagonist) has ~20-fold higher affinity at 5-HT_2A receptors over dopamine D₂ receptors, which makes it more efficacious against the negative symptoms of schizophrenia and less liable to causing extrapyramidal side effects than ‘typical’ antipsychotics.

The major goal of the current investigation was to study the structure of risperidone and to identify the minimum structural features required for 5-HT_2A receptor affinity that retain antagonist action. The structure of risperidone was systematically …

Emergent Interactions Influence Functional Traits And Success Of Dune Building Ecosystem Engineers, Joseph K. Brown

Theses and Dissertations

Stability of coastal systems are threatened by oceanic and atmospheric drivers of climate change. Sea-level rise compounded with increased frequency and intensity of storms emphasizes need for protection of inner island systems by dune formations. Dune building processes are affected by interactions between growth of ecosystem engineering dune grasses and environmental factors associated with disturbance such as sand burial and salt spray. Climate change may also cause latitudinal expansion of some species, resulting in emergence of competitive interactions that were previously absent. Topographic structure of coastlines, traditionally influenced by sand burial, could change as a result of competition emergence. My …

The Effect Of Pre-Deployment Physiology As A Predictor Of Post-Traumatic Stress Disorder Among A Sample Of United States Army National Guard And Reserve Soldiers, David J. Rothman

Theses and Dissertations

Potential risk factors for development of Post-Traumatic Stress Disorder (PTSD) are still unclear. One potential risk factor for the development of PTSD is an individual’s cardiovascular reactivity and recovery in response to stressor tasks. The current study was conducted with 763 Army National Guard and Army Reserve soldiers. Participants completed a stressful induction along with self-report measures prior to deployment. Post-deployment, self-report measures were completed to assess PTSD symptomatology and experiences related to deployment and combat. Multiple regression was used to determine the ability of blood pressure response to stress to predict PTSD symptoms immediately and one-year after return from …

Nitrogen Removal And Recycling By Sediments In The Lower Great Miami River, Ohio, Lee A. Slone

Browse all Theses and Dissertations

The Lower Great Miami River (LGMR) has recently experienced algal blooms due to excess nitrogen (N) and phosphorus (P). Some Ohio water treatment plants were ordered to reduce the amount of P that they release into the river, but N also can stimulate eutrophication and algal blooms, either alone or in combination with P. In this study, LGMR sediment N transformations and effects of river impoundments on N cycling were compared with external N loads during different flow conditions to determine the extent to which N is removed or recycled within the system. Sediment nutrient fluxes, sediment N sinks and …

Differentially Functionalized Silver Nanoparticles Alter K Metabolism In Human Red Blood Cells, Praveen Kumar Alla

Browse all Theses and Dissertations

Silver nano particles (AgNPs) are the most frequently used nanomaterials (NMs) in industrial and biomedical applications, and extensive knowledge about their properties is fast accumulating. In addition, functionalization of nanoparticles significantly impacts their properties and potential applications. In spite the urge to produce, investigate and apply them, the toxicity of nanoparticles and their interaction with blood components, such as erythrocytes, has been insufficiently investigated. Here, we report on the effect of AgNPs in cellular function, and particularly on ion transport in human red blood cells (HRBCs). Two methods of synthesis were used for the functionalization of AgNPs, Creighton, and Lee-Meisel. …

Implementation And Evaluation Of An Analytical Method For A Novel Derivatizing Agent To Measure 4,4'-Methylene Diphenyl Diisocyanate Atmospheres, Silvia Puscasu, Simon Aubin, Mark Spence, Sébastien Gagné

Études primaires

Accurate measurement of 4,4'-methylene diphenyl diisocyanate (MDI) atmospheres is a challenge since the molecule is both chemically reactive and likely to be present in aerosol form when heated and sprayed because of its low vapor pressure. Meeting this challenge requires optimizing both the sampling device used and the derivatization agent employed to stabilize the isocyanate functional group. This study describes the use of a novel derivatization reagent for isocyanate sampling to address the challenge of MDI aerosol exposure sampling. Like most conventional derivatizing agents for isocyanates, 1,8-diaminonapthalene (DAN) reacts with isocyanate functional groups to form a urea. However, unlike other …

Behaviors Of A Captive Coenobita Clypeatus In The Presence Of Varying Light Stimuli, Larry D. Fort, Ingrid K. Tulloch

Modern Psychological Studies

Coenobita clypeatus, or the purple pincher, is a terrestrial hermit crab and common pet in the United States. Modern behavioral studies have waned since the early 2000s; we therefore sought to characterize the behavior of a single captive C. clypeatus under varying light conditions. Frequency and duration of behaviors were studied in the presence of different light stimuli, along with weight monitoring. The crab was recorded for a set period of time every night with all conditions standardized except for light. The light stimulus alternated between no light, blue light, and red light. The case study revealed a significant increase …

Comparison Of Pea Seed Germination Due To Manipulation Of Soil Conditions, Ariel M. Kershner

Faculty Curated Undergraduate Works

This experiment was done with the intent to understand optimal soil conditions for pea seed germination. We investigated this using three soil conditions – 100% potting soil, 50% potting soil/50% sand, and 100% sand. Seemingly healthy pea seeds were planted and observed over five weeks. We measured both the total number of sprouts per week and the combined weight of the plants and seeds per condition before planting and after uprooting. We expected to find that the 100% potting soil condition would germinate the best, resulting in the most sprouts and heaviest weight of the condition’s seeds and plants. This …

Triclocarban Influences Antibiotic Resistance And Alters Anaerobic Digester Microbial Community Structure, Daniel Elliott Carey, Daniel Zitomer, Krassimira R. Hristova, Anthony D. Kappell, Patrick J. Mcnamara

Civil and Environmental Engineering Faculty Research and Publications

Triclocarban (TCC) is one of the most abundant organic micropollutants detected in biosolids. Lab-scale anaerobic digesters were amended with TCC at concentrations ranging from the background concentration of seed biosolids (30 mg/kg) to toxic concentrations of 850 mg/kg to determine the effect on methane production, relative abundance of antibiotic resistance genes, and microbial community structure. Additionally, the TCC addition rate was varied to determine the impacts of acclimation time. At environmentally relevant TCC concentrations (max detect = 440 mg/kg), digesters maintained function. Digesters receiving 450 mg/kg of TCC maintained function under gradual TCC addition, but volatile fatty acid concentrations increased, …

Epigenetic Response To Habitat Change: Changes Variation In Dna Methylation Frequencies And Generational Transmission Vary With Invasion Status, Aaron W. Schrey, Travis R. Robbins, Jacob D. Lee, David W. Dukes, Alexandria K. Ragsdale, Christopher J. Thawley, Tracy Langkilde

Biology: Faculty Publications

Epigenetic mechanisms may be important for a native species’ response to rapid environmental change. Red Imported Fire Ants (Solenopsis invicta Santschi, 1916) were recently introduced to areas occupied by the Eastern Fence Lizard (Sceloporus undulatus Bosc & Daudin, 1801). Behavioral, morphological and physiological phenotypes of the Eastern Fence Lizard have changed following invasion, creating a natural biological system to investigate environmentally induced epigenetic changes. We tested for variation in DNA methylation patterns in Eastern Fence Lizard populations associated with different histories of invasion by Red Imported Fire Ants. At methylation sensitive amplified fragment length polymorphism loci, we detected a higher …

Single-Particle Imaging Reveals Intraflagellar Transport–Independent Transport And Accumulation Of Eb1 In Chlamydomonas Flagella, J. Aaron Harris, Yi Liu, Pinfen Yang, Peter Kner, Karl F. Lechtreck

Biological Sciences Faculty Research and Publications

The microtubule (MT) plus-end tracking protein EB1 is present at the tips of cilia and flagella; end-binding protein 1 (EB1) remains at the tip during flagellar shortening and in the absence of intraflagellar transport (IFT), the predominant protein transport system in flagella. To investigate how EB1 accumulates at the flagellar tip, we used in vivo imaging of fluorescent protein–tagged EB1 (EB1-FP) in Chlamydomonas reinhardtii. After photobleaching, the EB1 signal at the flagellar tip recovered within minutes, indicating an exchange with unbleached EB1 entering the flagella from the cell body. EB1 moved independent of IFT trains, and EB1-FP recovery did …

Myc-Binding Protein Orthologue Interacts With Akap240 In The Central Pair Apparatus Of The Chlamydomonas Flagella, Venkatramanan G. Rao, Ruhi B. Sarafdar, Twinkle S. Chowdhury, Priyanka Sivadas, Pinfen Yang, Prabhakar M. Dongre, Jacinta S. D'Souza

Biological Sciences Faculty Research and Publications

Background

Flagella and cilia are fine thread-like organelles protruding from cells that harbour them. The typical ‘9 + 2’ cilia confer motility on these cells. Although the mechanistic details of motility remain elusive, the dynein-driven motility is regulated by various kinases and phosphatases. A-kinase anchoring proteins (AKAPs) are scaffolds that bind to a variety of such proteins. Usually, they are known to possess a dedicated domain that in vitro interacts with the regulatory subunits (RI and RII) present in the cAMP-dependent protein kinase (PKA) holoenzyme. These subunits conventionally harbour contiguous stretches of a.a. residues that reveal the presence of the …

Venom Expelled By Cottonmouths (Agkistrodon Piscivorus) Across Different Prey Sizes, Prey Taxa, And Snake Body Temperatures, Kari Lynn Spivey

Graduate Theses/Dissertations

Pit vipers possess a sophisticated venom delivery system enabling them to efficiently disable prey. To avoid retaliatory countermeasures, pit vipers typically release envenomated prey which are then trailed and consumed after succumbing to venom effects. Successful retrieval of released prey should vary with venom resistance and trail ability of prey types. The effects of prey size and prey type (mice, lizards, and frogs) on foraging behavior and venom expenditure in a cohort of juvenile cottonmouths was examined. Venom expenditure did not vary significantly among prey sizes or prey types. However, lizard prey were held significantly more often than mice. The …

Quantitative Trait Loci (Qtl) Analysis Of Sulfur Sensitivity In Vitis Aestivalis-Derived 'Norton', Logan Michael Duncan

Graduate Theses/Dissertations

The grape and wine industry relies heavily on sulfur and sulfur containing compounds to control the fungal disease powdery mildew (PM). Sulfur dioxide induces large-scale transcriptomic modification in the Eurasian grape species Vitis vinifera with little to no phytotoxicity. However, genetic factors contributing to sulfur sensitivity, characterized by vegetative necrosis and defoliation, remain undefined in North American grape species and their commercial hybrids. A mapping population, consisting of 147 F1 genotypes, was created by crossing V. aestivalis-derived ‘Norton’ and V. vinifera ‘Cabernet Sauvignon’ to identify the genetic basis for sulfur sensitivity in North American wine grapes. Clonally propagated F1 genotypes …

Friends In Low Places: Responses Of A Benthic Stream Fish To Intra-Guild Alarm Cues, Kelsey Ann Anderson

Graduate Theses/Dissertations

Many aquatic species produce chemical alarm cues that serve as a warning to nearby conspecifics. In mixed-species aggregations, individuals may also benefit by “eavesdropping” on the chemical alarm cues of other species that are in the same prey-guild. Rainbow Darters (Etheostoma caeruleum) are benthic fish that co-occur with native Ozark Minnows (Notropis nubilus), recently-introduced Western Mosquitofish (Gambusia affinis), and native Oklahoma Salamanders (Eurycea tyrnerensis), all of whom are vulnerable to the same predators. We tested the responses of darters to the damage-released alarm cues of conspecifics (positive control), minnows, and mosquitofish; alarm cues from Bumblebee Gobies (Brachygobius doriae) served as …

Natural History And Conservation Of Bolitoglossine Salamanders In Central Panama, Leslie Caren Brinkman

Graduate Theses/Dissertations

I conducted a mark-recapture study to assess the natural history of a Neotropical salamander community in three discrete 4–6 week sampling periods at Chorro Las Yayas, a protected natural area of about 8000 m2 in central Panama, during an extended drought. I captured 87 Bolitoglossa biseriata, 17 Oedipina complex, and 15 O. parvipes. Bolitoglossa biseriata, had a mean mass of 0.8 ± 0.4 g with a mean snout-vent length (SVL) of 31.45 ± 5.30 mm. Bolitoglossa biseriata was active throughout the night, and its detection rate varied with season, air temperature, and amount of precipitation in the previous 24 h. …

Behavioral Responses By Cottonmouths (Agkistrodon Piscivorus) To Chemical And Visual Predator Cues, Kristen Lee Kohlhepp

Graduate Theses/Dissertations

Predator detection and assessment of predation risk have important survival consequences for animals. However, responses to predatory threats can vary with different stimuli. Chemical cues are important for predator recognition but visual cues may elicit defensive responses. Cottonmouths (Agkistrodon piscivorus) exhibit an array of antipredatory behaviors that have been thoroughly characterized in response to human aggressors, but their responses to cues from other predators are poorly known. I conducted three experiments to evaluate cottonmouth responses to visual and chemical stimuli from predators and non-predators. Snakes exposed to predator chemical cues exhibited elevated tongue-flick rates compared to controls but did not …

Size-Selective Predation By Ringed Crayfish (Orconectes Neglectus) On Native And Invasive Snails, Whitney Marie Kelley

Graduate Theses/Dissertations

As invasive species are introduced into new habitats, predator-prey interactions can be altered. Cipangopaludina chinensis is a large snail that has been introduced into numerous waterbodies throughout the United States, where it coexists with native snails and their crayfish consumers. This study examined the relative susceptibility of C. chinensis and two native snails (Elimia potosiensis and Physella gyrina) to predation by the ringed crayfish (Orconectes neglectus). I conducted field surveys of snails and crayfish in three Ozark streams, plus a series of four lab experiments to determine feeding rates and prey preference by O. neglectus on these three snail species. …

Trophic Interactions And The Efficacy Of Milfoil Weevils For Biocontrol Of Eurasian Watermilfoil In Wisconsin Lakes, Kristopher Andrew Maxson

Graduate Theses/Dissertations

Eurasian water-milfoil (Myriophyllum spicatum L., henceforth “EWM”) is the most heavily managed nuisance submersed aquatic plant in the United States. EWM’s rapid spring growth and formation of dense surface mats inhibits native macrophyte communities, serves as poor-quality habitat for fish and macroinvertebrates, impacts recreation, and can clog water supply infrastructure. The milfoil weevil (Euhrychiopsis lecontei Dietz) has been associated with EWM declines in several states, though natural weevil densities are generally too small to effect control. Augmentative biocontrol has had varied success and fish predation may account for high weevil mortality. Weevils were augmented in 4 northern Wisconsin lakes in …

Facilitating A Change Of Mind About Father God Through Mentoring Of The Fatherless, Stephen Madosky

Doctoral Dissertations and Projects

The consensus of published research has proven beyond question that fatherlessness is a crisis in America. Textbooks and autobiographies have covered and reported the statistical effects of fatherlessness; nonetheless, there is far less literature dealing with the solution of fatherlessness and even less literature from Church scholars on how the Church may participate in its correction. This thesis presents an understanding of fatherlessness and proposes a resolution to act on behalf of fatherless children. The resolution will be comprised in preparing Church leaders to equip Church laity to understand and to minister to the fatherless in their affliction effectively.

Spiritual Nurture In Developing The Faith Of Christian High School Students: A Phenomenological Study, Martha Boyd-Mitchell

Doctoral Dissertations and Projects

Many Christian schools provide solid academic environments for adolescents. The programming promises to deliver an education to nurture the human soul. This qualitative, transcendental, phenomenological study was for the purpose of discovering how spiritual nurture in Christian high schools aided and encouraged faith development. Christian high school is the lived experience of adolescents going to school with spiritual nurture at the heart of their programming. The participants were 15 adolescents who attended a Christian high school that implemented spiritual nurture by use of prayer, worship, biblically-integrated curriculum, Bible study, teacher-student relationships, holistic correction, and peer relationships. The epoche was the …

Spiritual Nurture In Developing The Faith Of Christian High School Students: A Phenomenological Study, Marsha Boyd-Mitchell

Doctoral Dissertations and Projects

Many Christian schools provide solid academic environments for adolescents. The programming promises to deliver an education to nurture the human soul. This qualitative, transcendental, phenomenological study was for the purpose of discovering how spiritual nurture in Christian high schools aided and encouraged faith development. Christian high school is the lived experience of adolescents going to school with spiritual nurture at the heart of their programming. The participants were 15 adolescents who attended a Christian high school that implemented spiritual nurture by use of prayer, worship, biblically-integrated curriculum, Bible study, teacher-student relationships, holistic correction, and peer relationships. The epoche was the …

Parental Concerns Surrounding The Care And Education Of Military Dependent Children With Autism: A Phenomenological Study, Charles Tidwell

Doctoral Dissertations and Projects

Autism continues to be a growing concern in America and there is indication that the rate of autism among the sub-population of military dependent children is potentially higher than the general public. This hermeneutic phenomenological study focused on the concerns and experiences of parents of military families raising and educating their children with autism. Data was collected from nine military families across the United States. Six fathers and seven mothers participated through an initial questionnaire, interview, and a written reflective timeline. Findings demonstrated the stress, strain and sacrifice of families serving in the military with children with autism. Continuity of …

Fluoroscopic System For Assessment Of In Vivo Hindfoot Kinematics During Gait: Control Data And Applications In Osteogenesis Imperfecta, Ben Mchenry, Jason Long, Peter Smith, Haluk Altiok, Gerald F. Harris

Biomedical Engineering Faculty Research and Publications

No abstract provided.

Nih Visible Human Project Humerus Numerical And Physical Models: Characterization For Osteogenesis Imperfecta Fracture Study, Prateek Grover, Gerald F. Harris

Biomedical Engineering Faculty Research and Publications

No abstract provided.

Ecological Effects Of Nutrient Enrichment On A Coastal Fringe Mangrove Forest, Brita Juliet Jessen

Open Access Dissertations

Mangrove ecosystems are highly productive coastal wetlands that connect tropical terrestrial and marine systems. Increasing human populations and land use changes are enhancing nutrient loading to tropical coastlines, potentially leading to eutrophic conditions for marine systems. The carbon (C) cycle is critical for mangrove forest structure, function, and resilience under environmental change. Increasing trends of nutrient enrichment in tropical coastal waters may have an effect on organic C mineralization by alleviating nutrient limitation of the heterotrophic microbial community, which may lead to a loss of within-stand carbon sequestration. To better understand and predict the consequences of increased nutrient input, a …