Digital Commons Network^™

Probing The Plant Cell Wall With Herbicides: A Chemical Genetics Approach, Chad B. Brabham

Theses and Dissertations--Plant and Soil Sciences

The primary cell wall is a highly organized multi-layered matrix of polysaccharides (cellulose, hemi-cellulose, and pectin). The ability of the rigid cell wall to sufficiently loosen to allow growth is a complex process that differs considerably between grasses monocots and dicots. Cellulose is the major structural component required for anisotropic cell expansion and is synthesized by CELLULOSE SYNTHASE A (CesA) proteins. Here, our objectives were two-fold: 1) dissect cell walls and cellulose biosynthesis in dicots and grasses using chemical biology and reverse genetic approaches 2) characterize and classify the inhibitory mechanisms of cellulose biosynthesis inhibitors (CBIs). A reverse genetics TILLING …

Transcriptional Regulation Of Specialized Metabolites In Arabidopsis Thaliana And Catharanthus Roseus, Craig M. Schluttenhofer

Theses and Dissertations--Plant and Soil Sciences

For millennia humans have utilized plant specialized metabolites for health benefits, fragrances, poisons, spices, and medicine. Valued metabolites are often produced in small quantities and may command high prices. Understanding when and how the plant synthesizes these compounds is important for improving their production. Phytohormone signaling cascades, such as jasmonate (JA) activate or repress transcription factors (TF) controlling expression of metabolite biosynthetic genes. TFs regulating specialized metabolite biosynthetic genes can be manipulated to engineer plants with increased metabolite production.

WRKY transcription factor are known components of both JA signaling cascades and regulation of specialized metabolism. The presence of WRKY binding …

The Effects Of Manufactured Nanomaterial Transformations On Bioavailability, Toxicity And Transcriptomic Responses Of Caenorhabditis Elegans, Daniel L. Starnes

Theses and Dissertations--Plant and Soil Sciences

In recent decades, there has been a rapid expansion in the use of manufactured nanoparticles (MNPs). Experimental evidence and material flow models predict that MNPs enter wastewater treatment plants and partition to sewage sludge and majority of that sludge is land applied as biosolids. During wastewater treatment and after land application, MNPs undergo biogeochemical transformations (aging). The primary transformation process for silver MNPs (Ag-MNPs) is sulfidation, while zinc oxide MNPs (ZnO-MNPs) most likely undergo phosphatation and sulfidation. Our overall goal was to assess bioavailability and toxicogenomic impacts of both pristine, defined as-synthesized, and aged Ag- and ZnO-MNPs, as well as …

Estrogen Signaling And Endocrine Disruption Potential In The Embryonic Development Of The Female Reproductive Tract Of The American Alligator, Alligator Mississippiensis, Brenna M. Doheny

MUSC Theses and Dissertations

Perturbation of endocrine signaling during critical embryonic developmental windows has been implicated in many female reproductive system disorders. Reproductive tract anomalies in women exposed in utero to synthetic estrogen diethylstilbestrol, prescribed as a miscarriage preventative, provided some of the first evidence for this “embryonic origins of adult disease” paradigm and suggested a role for estrogen signaling in female reproductive tract (FRT) differentiation. Developmental studies of FRT in other vertebrates have great value in furthering our understanding of embryonic origins of reproductive disorders. The alligator is a particularly intriguing model as an environmental sentinel species with temperature dependent sex determination, which …

Stem In General Education: Does Mathematics Competence Influence Course Selection, Mary C. Enderson, John Ritz

STEMPS Faculty Publications

Many students enroll in college programs to prepare for their future careers. All are required to complete general studies courses. At one university, technology and STEM courses fulfill a part of the natural science and technology general education requirements. This study uses a survey design to explore why 332 students chose to enroll in a STEM technology course. Results found most enroll because their advisor suggests the course, it meets a general education major requirement, and the course is offered at a convenient time. Fewer enroll in the course because they would like to find out more about STEM fields, …

Comparing High School Students' And Adults' Perceptions Of Technology, Henry L. (Hal) Harrison Ii, Philip A. Reed

STEMPS Faculty Publications

This study compared high school student’s perceptions of technology and technological literacy to those perceptions of the general public. Additionally, individual student groups were compared statistically to determine significant differences between the groups. The ITEA/Gallup Poll instrument was used to survey high school student’s perceptions of technology in the study. The student population in question consisted of three subgroups: students enrolled in a standards-based technology education courses, students enrolled in a Project Lead the Way (PLTW) Principles of Engineering pre-engineering course, and students enrolled in a general education course (language arts, mathematics, or science). In addition, each student group’s perceptions …

Involuntary Consent: Conditioning Access To Health Care On Participation In Clinical Trials, Ruqaiijah A. Yearby

All Faculty Scholarship

Although the controversy over the lack of consent in fetal-tissue clinical trials is relatively new, history is replete with instances of medical researchers who have conducted clinical trials with minorities and the economically disadvantaged without their consent.1 Traditionally, American bioethics has served as a safety net for the rich and powerful (for they are not forced to act as research subjects to obtain access to health care for themselves or their children) while failing to protect the vulnerable, which includes minorities and the economically disadvantaged. Without access to health care, minorities and the economically disadvantaged are unduly influenced to participate …

Missing The “Target”: Preventing The Unjust Inclusion Of Vulnerable Children For Medical Research Studies, Ruqaiijah A. Yearby

All Faculty Scholarship

Nearly everyone has experienced a burn and the resulting pain. Now imagine that you suffer a third-degree radiation burn that injures all the layers of your skin as well as the tissue, causing you extreme pain. . The burn turns your skin white, cherry red, or black and may produce blisters that are dry, hard, and leathery-looking. The burn can also be seen on the surface of your lungs and gastrointestinal tract. If the burn is big enough you will need skin grafts and surgery to replace the skin and tissue that will never grow back, as well as treatment …

Phenotype And Function Of Cd209+ Bovine Blood Dendritic Cells, Monocyte-Derived- Dendritic Cells And Monocyte-Derived Macrophages, Kun Taek Park, Mahmoud M. Elnaggar, Gaber S. Abdellrazeq, J. P. Bannantine, Victoria Mack, Lindsay M. Fry, William C. Davis

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Phylogenic comparisons of the mononuclear phagocyte system (MPS) of humans and mice demonstrate phenotypic divergence of dendritic cell (DC) subsets that play similar roles in innate and adaptive immunity. Although differing in phenotype, DC can be classified into four groups according to ontogeny and function: conventional DC (cDC1 and cDC2), plasmacytoid DC (pDC), and monocyte derived DC (MoDC). DC of Artiodactyla (pigs and ruminants) can also be sub-classified using this system, allowing direct functional and phenotypic comparison of MoDC and other DC subsets trafficking in blood (bDC). Because of the high volume of blood collections required to study DC, cattle …

Evolution And Biogeography Of Haemonchus Contortus: Linking Faunal Dynamics In Space And Time, Eric P. Hoberg, D.S. Zarlenga

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

History is the foundation that informs about the nuances of faunal assembly that are essential in understanding the dynamic nature of the host-parasite interface. All of our knowledge begins and ends with evolution, ecology and biogeography, as these interacting facets determine the history of biodiverse systems. These components, relating to Haemonchus, can inform about the complex history of geographical distribution, host association and the intricacies of host-parasite associations that are played out in physiological and behavioural processes that influence the potential for disease and our capacity for effective control in a rapidly changing world. Origins and evolutionary diversification among species …

Enhanced Approaches For Identifying Amadori Products: Application To Peanut Allergens, Katina L. Johnson, Jason G. Williams, Soheila J. Maleki, Barry K. Hurlburt, Robert E. London, Geoffrey A. Mueller

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The dry roasting of peanuts is suggested to influence allergic sensitization as a result of the formation of advanced glycation end products (AGEs) on peanut proteins. Identifying AGEs is technically challenging. The AGEs of a peanut allergen were probed with nano-scale liquid chromatography−electrospray ionization−mass spectrometry (nanoLC−ESI−MS) and tandem mass spectrometry (MS/MS) analyses. Amadori product ions matched to expected peptides and yielded fragments that included a loss of three waters and HCHO. As a result of the paucity of b and y ions in the MS/MS spectrum, standard search algorithms do not perform well. Reactions with isotopically labeled sugars confirmed that …

The Worm’S Eye View Of Community Ecology, Tavis K. Anderson, Michael V. K. Sukhdeo

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The study of parasites in the context of community level organization, either as parasites embedded within host communities, or as parasite communities themselves, is now quite prevalent in parasitology and ecology today. However, this was not always the case. In terms of publications, there was almost no consideration of parasite interactions at the community level for most of the first half of the last century. Papers in The Journal of Parasitology by Clark Read (1951) and John Holmes (1961) were the defining contributions to the beginning of the field, and the ideas elaborated by these two parasitologists still inspire current …

Rising Atmospheric Co2 Is Reducing The Protein Concentration Of A Floral Pollen Source Essential For North American Bees, Lewis H. Ziska, Jeffery S. Pettis, Joan Edwards, Jillian E. Hancock, Martha B. Tomecek, Andrew Clark, Jeffrey S. Dukes, Irakli Loladze, H. Wayne Polley

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

At present, there is substantive evidence that the nutritional content of agriculturally important food crops will decrease in response to rising levels of atmospheric carbon dioxide, C_a. However, whether C_a-induced declines in nutritional quality are also occurring for pollinator food sources is unknown. Flowering late in the season, goldenrod (Solidago spp.) pollen is a widely available autumnal food source commonly acknowledged by apiarists to be essential to native bee (e.g. Bombus spp.) and honeybee (Apis mellifera) health and winter survival. Using floral collections obtained from the Smithsonian Natural History Museum, we quantified C_{a …}

I Am Adele Bloch-Bauer, I Am Hester Prynne, Laurie Lico Albanese Mfa

All Student Scholarship

I AM ADELE BLOCH-BAUER, I AM HESTER PRYNNE is a compilation of fiction and nonfiction. This cross-genre thesis includes two excerpts from historical novels with female protagonists, and an essay on women’s historical fiction. For the study and creation of female-centered historical fiction I researched and wrote in a wide range of areas, both intellectual and temporal. First, I read and traced the emergence of female-focused American historical fiction that began with Nathaniel Hawthorne’s The Scarlet Letter, and continues today with historical fiction based in fact such as Lily King’s Euphoria and Paula McClain’s The Paris Wife and Circling the …

Currency, Elisha M. Emerson Mfa

All Student Scholarship

The privileged Watters family, David, Winnie, and their 14-year-old daughter, Faye, struggle to adjust their suburban Charlotte lifestyle to fit a much-reduced income. Their fast failure leads them down separate paths: David after enlightenment through Transcendental Meditation, Faye after the power she feels in the company of her handsome Earth Science teacher, and Winnie in a romantic foray to Alaska after buried gold. Currency probes that moment when everything changes size, when initial annoyances shrink and reality resumes a new and disturbing sense of proportion.

Spectrum 2016, Saint Mary's College Of California

The Undergraduate Spectrum

No abstract provided.

Battle On The Homefront: An Auto-Ethnographic Perspective On Domestic Violence Post-Deployment, Michelle Craske

Electronic Theses and Dissertations

Domestic and sexual trauma that occurs during the time a soldier is actively serving in the military are difficult for researchers to measure in large part because the Department of Defense does not report these offenses to the public. Recent combat missions to the Middle East have increased mental health issues among soldiers, but it is unclear whether these issues are related to domestic violence and sexual trauma soldiers may have endured. The purpose of this study is to investigate how combat operations may have increased domestic violence and sexual trauma among soldiers. An auto ethnographic method is used to …

A Tale Of Two Palmettos: The Foundation Of Ecosystems, Warren G. Abrahamson Ii

Faculty Journal Articles

Fruits of saw palmetto (Serenoa repens) provide crucial, high-quality resources to wildlife while providing a matrix for many southeastern US ecosystems. As a foundation species, saw palmetto plays a major role in structuring fire-maintained plant communities. Often living on nutrient-poor soil conditions, the thick leaves of saw palmetto are long lived, up to 2.8 years in shaded sand pine scrub.

Multiple Records Of Monoecy In Dioecious Taxa Of Hawaiian Coprosma Spp. (Rubiaceae)., Jason T. Cantley, Danielle Frohlich, Chris Martine

Faculty Journal Articles

The breeding systems of the 110+ species of Coprosma J.R. Forst. & G. Forst. across the Pacific are largely considered dioecious, with male and female flowers occurring on separate plants (oliver 1935). However, for many species, particularly of New Zealand, the dioecious breeding system is leaky. Coprosma species exhibiting leaky dioecy are primarily dioecious, with male and female flowers on separate plants, but individuals occasionally develop apparently functional bisexual flowers, which sporadically occur among an abundance of unisexual flowers. A putative monoecious Coprosma taxon was once reported for the Macquarie Island individuals of C. perpusilla Colenso (= C. pumila Hook.f.), …

Evidence Of Rock Kangaroo Seed Dispersal Via Faecal Seed Storage In A Tropical Monsoon Community, Chris Martine, Ally Boni, Elizabeth Capaldi, Gemma Lionheart, Ingrid E. Jordon-Thaden

Faculty Journal Articles

While some of the plant species of the ‘Sandstone Country’ along the escarpment of western Arnhem Land produce fleshy fruits and appear to rely on biotic methods of seed dispersal, little is known about the methods by which this is achieved – and few potential dispersers co-occur in the sandstone outcrop communities. For the present study, scat collections were made on outcrops in the northeastern area of Kakadu National Park with the hope of uncovering relationships between local frugivores and fruit-producers, and providing evidence for seasonal storage of mammal-dispersed seeds in scat prior to germination. The goals of the present …

Visual Scanpath Training For Facial Affect Recognition In A Psychiatric Sample, Chi Chan

Electronic Theses and Dissertations

Social cognition is essential for functional outcome and quality of life in psychiatric patients. Facial affect recognition (FAR), a domain of social cognition, is impaired in many patients with schizophrenia and bipolar disorder. There is evidence that abnormal visual scanpath patterns may underlie FAR deficits, and metacognitive factors may impact task performance. The present study aimed to develop a brief, individually-administered, computerized training program to normalize scanpath patterns in order to improve FAR in patient with a psychosis history or bipolar I disorder. The program was developed using scanpath data from 19 nonpsychiatric controls (NC) while they completed a FAR …

Preparing For The Inevitable: Sensemaking In Parent-Child Discussions Of Death, Carrie Ann Wartmann

Electronic Theses and Dissertations

Death is something everyone will eventually encounter, yet American society has a tendency to avoid or deny death in everyday life and language. Death makes people uncomfortable, and many view it as a topic too complex for children to understand. Children, however, witness big and little deaths in their lives: of pets, relatives, plants, and favorite fairy tale characters. When a child experiences a death, he or she may have questions for parents or other trusted adults which our current avoidance-geared society does not prepare adults for. Children exist in a specific cultural context, and learn rules and expectations of …

The Owl Sensor: A Smart Nanostructure For Single Nucleotide Variation Analysis, Rebekah Karadeema

Electronic Theses and Dissertations

Analysis of single nucleotide variations (SNVs) in DNA and RNA sequences is extensively used in healthcare for detection of genetic mutations and analysis of drug resistant pathogens. Here we developed a nucleic acid sensor able to differentiate between a fully matched analyte and one with a SNV in a wide temperature range of 5°C-32°C. The sensor, dubbed here the 'Owl Sensor' due to the complex's resemblance to owl eyes, utilizes recent developments in DNA nanotechnology and synthetic biology to self-assemble a fluorescent DNA nanostructure called a Double Crossover, or DX Tile, capable of differentiating SNVs in a large temperature range, …

Examination Of The Relationship Between Caesarean Section Births And Attention Deficit Hyperactivity Disorder, Breea M. Rosas

All Master's Theses

Attention Deficit/Hyperactivity Disorder, a neurological disorder characterized by inattentive and hyperactive behaviors, has become increasingly more prevalent (CDC, 2010). Recent research has found associations between this disorder and Caesarean section births (Amiri et al., 2012; Juarez et al., 2008). The current study examined the relationship between C-section birth and self-reported symptoms of ADHD by college students on the Internal Restlessness Scale (Weyandt et al., 2003). Results of the study indicate a significant difference in mean ratings of ADHD symptoms between C-section and vaginally born participants (t = 3.683, p < .000). The current study supports previous findings of a relationship between C-sections and ADHD.

Heterogeneity Influences On Stream Water–Groundwater Interactions In A Gravel-Dominated Floodplain, R. B. Miller, Derek M. Heeren, Garey A. Fox, Todd Halihan, Daniel E. Storm

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Floodplains are composed of complex depositional patterns of ancient and recent stream sediments, and research is needed to address the manner in which coarse floodplain materials affect stream/groundwater exchange patterns. Efforts to understand the heterogeneity of aquifers have utilized numerous techniques typically focused on point-scale measurements; however, in highly heterogeneous settings, the ability to model heterogeneity is dependent on the data density and spatial distribution. The objective of this research was to investigate the correlation between broad-scale methodologies for detecting heterogeneity and the observed spatial variability in stream/groundwater interactions of gravel-dominated alluvial floodplains. More specifically, this study examined the correlation …

Incorporation Of Globally Available Datasets Into The Roving Cosmic-Ray Neutron Probe Method For Estimating Field-Scale Soil Water Content, William A. Avery, Catherine Finkenbiner, Trenton E. Franz, Tiejun Wang, Anthony L. Nguy-Robertson, Andrew E. Suyker, Timothy J. Arkebauer, Francisco Munoz-Arriola

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The need for accurate, real-time, reliable, and multi-scale soil water content (SWC) monitoring is critical for a multitude of scientific disciplines trying to understand and predict the Earth’s terrestrial energy, water, and nutrient cycles. One promising technique to help meet this demand is fixed and roving cosmic-ray neutron probes (CRNPs). However, the relationship between observed low-energy neutrons and SWC is affected by local soil and vegetation calibration parameters. This effect may be accounted for by a calibration equation based on local soil type and the amount of vegetation. However, determining the calibration parameters for this equation is labor- and time-intensive, …

Species Identification Of The Stylohyoid Bone For North American Artiodactyls, Thomas A. Hale

All Master's Theses

Zooarchaeologists cannot identify mammal species by their stylohyoid bones. Current trends in zooarchaeological research stress the need for rigorous and accessible identification methodology. I examined the stylohyoids of 15 hooved mammals: cattle, bison, domestic sheep, bighorn sheep, Dall sheep, mountain goat, domestic goat, elk, caribou, white-tailed deer, mule deer, moose, pronghorn antelope, domestic pig, and horse. Objectives included documenting how to side the stylohyoid (left or right), and producing species identification criteria based on large samples. A total of 325 samples were measured from eight repositories. Written descriptions, photographs, and success ratios for metrics and distinct traits are included for …

An Economic Approach To Modeling Archaeological Settlement Patterns In Central Idaho, Anthony J. Saunders

All Master's Theses

Archaeologists can gain a better understanding of subsistence strategies by analyzing the net advantage of exploiting certain resources over others across a large area with the use of Geographic Information Systems (GIS) and application of economic. GIS modeling is a powerful tool used by archaeologists to catalog and analyze site information in a spatial context. Economic models interpret human behavior in terms of cost and benefit. Little archaeological research has been done in central Idaho. This thesis develops economic models of hunting, gathering and fishing for the Frank Church River of No Return Wilderness (FC-RONRW). This research builds upon previous …

Self-Compassion, Mindfulness And Wellbeing In Counselors-In-Training, Lea Fairbanks

All Master's Theses

The purpose of this study was to examine if self-compassion and mindfulness predict wellbeing in counselors-in-training. The sample was made up of counselors-in-training from CACREP accredited programs in the WACES region of the United States (N=45). Data was collected from three scales including the Self-Compassion Scale, Five Facet Mindfulness Scale and the Psychological Wellbeing Scale. To analyze the data, a simultaneous regression was used to determine if self-compassion and mindfulness significantly predicted wellbeing in counselors-in-training. The results showed that self-compassion and mindfulness was positively correlated with wellbeing and significantly predicted 64.9% of the variance in wellbeing for counselors-in-training. The results …

Effect Of Quantum Dots, Novel Biological Imaging Agents, In Liver Cells In Vitro, Utshaha Maharjan

All Master's Theses

The use of Quantum dots (QDs) coated with polymer and functionalized with carboxylic acid groups in medical applications are explored. Their water solubility and exceptional stability in aqueous environments make them potentially useful for such applications as imaging and ligand attachments. However, there are concerns regarding the toxic effects of QDs and the minimal dose that can be used without producing any detrimental effects to organisms. In this study, QDs coated with the amphiphilic polymer coating tri-n-octylphosphine oxide and poly (maleic anhydride-alt-1-tetradecene (TOPO-PMAT)) which is functionalized with carboxylic acid groups were used to investigate their toxic effect in mouse liver …