Digital Commons Network^™

Race Relations In America: Complacency All Along?, Seong C. Kang

Journal of Public Management & Social Policy

No abstract provided.

The Ugly Side Of America: Institutional Oppression And Race, Renita Seabrook, Heather Wyatt-Nichol

Journal of Public Management & Social Policy

This manuscript examines structural racism through a socio-historical context of institutional oppression and its effects on modern society. The epistemological framework of intersectionality is used to focus on the overlap of oppression, structural racism, and implicit bias evident in the stereotypes and perceptions of the African American male population in the United States. Four eras of socio-historical significance are addressed: 1. Foundations of Racial Oppression; 2. Racism: Reconstruction and Jim Crow; 3. Renewal: Civil Rights and Civil Disobedience; 3. Reckoning: Embedded Racism and the Criminal Justice System.

Out Of The Recent Darkness And Into The New Light: Managerial Implications Emerging From The Martin-Zimmerman Encounter, Brian N. Williams, Billy R. Close, Seong C. Kang

Journal of Public Management & Social Policy

This paper revisits the encounter between George Zimmerman and Trayvon Martin from two perspectives: the co-production of public safety and public order and black crimmythology. Co-production is associated with the expanding and often unpredictable role that community residents, formal and informal communal organizations and nongovernmental institutions play in assisting public agencies in developing and implementing public services (Whitaker 1980; Parks et al. 1981). Black crimmythology is a term used to describe the historical and contemporary conflation of blackness, maleness, and criminality in the mind of the American public (Close 1997). The objectives of this analysis goes beyond ascertaining the guilt, …

Growth Of Cyanobacteria Anabaena Variabilis On Jsc-1a, A Martian Regolith Simulant, Stewart Morely

Utah Space Grant Consortium

Mars is a planet. Freezing temperatures, harsh solar, and a lack of water have left the surface of the planet sterile with no hope for even the hardiest of life forms to emerge. However, if Mars was warmer and had an atmosphere; could life emerge? In this project, we study how a martian regolith analog affects the growth of the cyanobacteria Anabaena variabilis. Our results indicate that in appropriate quantities, the martian material may aid growth by providing extra minerals in the growth media, however at higher concentrations these minerals become chemotoxic. We also test whether the martian regolith simulant …

Improved Platform Design For Organic Particle Detection Using Buried Channel Waveguides, Thomas Wall

Utah Space Grant Consortium

This paper discusses the ARROW biosensor fabricated at Brigham Young University. The biosensor is integrated optofluidic device that is capable of individual organic particle detection. The biosensor was included in the Biological and Life Detection (BOLD) mission to Mars proposal for use in sensing any organic material left in the soil on Mars. The paper discusses the need for a high sensitivity for the biosensor and discusses on major design change that will improve the biosensor’s performance – changing the ridge waveguide used historically to a buried channel waveguide. The buried channel waveguide protects the waveguide from any detrimental water …

Four President's Award Recipients Recognized At May 7 Undergraduate Commencement, Winthrop University

Winthrop News 2016

• President Dan Mahony announced that John W. Barrera, Lauren A. Goodwin, Zane M. Repp and Kathryn V. Steverson earned the President's Award.
• Both Barrera and Steverson will pursue medical degrees - Barrera at the University of South Carolina program in Greenville and Steverson at the USC program in Columbia.
• Repp plans to apply for clinical psychology graduate programs, while Goodwin wants to earn a master’s degree in higher education student affairs.

Volume 51 - Issue 24 - Monday, May 9, 2016, Rose Thorn Staff

The Rose Thorn Archive

The Rose Thorn, Rose-Hulman's independent student newspaper.

Sr-15-16-54 Cc, Marshall University

Recommendations

No abstract provided.

Cwu General Education Committee Minutes - 05/09/16, Janet Shields

All Faculty Committee Minutes

These are the official Central Washington University General Education Committee minutes for the May 9, 2016 meeting.

Commencement Program 2015 May Undergraduate, Gcsu Special Collections

Commencement Programs

No abstract provided.

Effect Of Using Culturally Sensitive Education In Increasing Mammogram Use And Breast Cancer Awareness: African Female Immigrants, Gertrude Mumba-Kaunda

Evidence-Based Practice Project Reports

Despite the advances in medical technology, breast cancer is the most common cancer among women and is the second leading cause of death in African-American and Caucasian women in the United States. Mammography has shown to be one of the best methods to reduce late detection of breast cancer. The American Cancer Society recommends monthly self-breast examination (SBE), clinical breast examinations every 3 years, and yearly mammography starting at the age of 40. Despite the recommendations, there is still significant disparity among different racial groups, when it comes to death rates, which can be attributed to low screening rates. The …

Ua12/2/1 Graduation Edition, Wku Student Affairs

WKU Administration Documents

Special commencement edition of the College Heights Herald.

Building Quality Novel Formats And Development Processes, Steven Lang

Cell Culture Engineering XV

There has been an explosion of interest in bispecific or multi-specific bio therapeutic formats. Monospecific antibodies remain the foundation for most bispecific formats. However, monospecific strategies cannot be directly translated into the discovery and development of bispecific antibodies. Bispecifics present unique challenges especially for efficient and consistent assembly/production processes and analytical methods to ensure high quality drug products. Improvements in selection of bispecific combinations/architecture, yield and efficiency and common analytical issues and resolutions will be discussed. Applying improved methods learned from Fab-arm exchange bispecifics and alternative scaffold-antibody fusions, we have established a foundation of knowledge that enables accelerated development

Microfluidic Accelerated Evaluation Of Cho Cell Clones By Perfusion Of Fed-Batch Conditioned Media, Darek Sikorski

Cell Culture Engineering XV

The generation of genetically engineered production CHO cell lines is normally the longest step in the race to scale-up protein manufacturing. This labor-intensive screening includes the expansion of hundreds of clonal cultures to sufficient numbers for their growth and productivity to be evaluated. Unfortunately, many clones that perform well when screened at the batch cloning stage display reduced performance under fed-batch conditions. Thus, screening potential clones under conditions more similar to the ultimate production cultures provides an opportunity for more effective clone selection.

We combined the advantages of precise measurements in microfluidic nL volume cultures with our ability to modify …

Systems Engineering Of A Cho Cell Line For Enhanced Process Robustness, Kyoungho Lee, Hsu-Yuan Fu, Liang Zhao, Lei Feng, Tung Le, Haiyun Pei, Sugandha Rajput, Dong Cho, Nikunj Somia, Scott Mcivor, Wei Hu

Cell Culture Engineering XV

Recent advances in genome engineering have opened great opportunities for engineering Chinese hamster ovary cells. An ideal cell line is no longer just one with high productivity, but also with high stability in both the productivity and product quality, and in both extensive passaging and long term continuous culture. Furthermore, an ideal cell line should be provided with process controllability, allowing the use of environmental control variables to steer its metabolism to a reaction pathway that favors the synthesis of product with the desired quality attributes. Importantly, these superior traits must be genetically and epigenetically stable and be passed on …

Evolution Of An Integrated Continuous Antibody Manufacturing Process, Christopher Kistler

Cell Culture Engineering XV

Efforts continue to reduce the timeline and cost of progressing biologics from discovery through preclinical development to product launch. Integrated processes, those that link upstream and downstream processing with control of product quality are an attractive way to achieve meaningful reductions in the overall cost of ownership of a biologics manufacturing process.

To start along this path, an integrated continuous processing laboratory, the PROLab (Protein Refinery Operations Lab) was built to pilot the concepts of an integrated continuous antibody manufacturing process under automated control. A perfusion process was developed capable of operating for >30 days using either TFF (Spectrum KrosFlo® …

Reduction Of Metabolic Waste Products, Ammonia And Lactate, Through The Coupling Of Gs Selection And Ldh-A Down-Regulation In Cho Cells, Soo Min Noh, Jin Hyoung Park, Gyun Min Lee

Cell Culture Engineering XV

The cultivation of Chinese hamster ovary (CHO) cells for the production of therapeutic proteins inevitably accompanies the production of metabolic wastes, mostly ammonia and lactate. Ammonia alters cell growth, productivity and the glycosylation patterns of proteins, and lactate acidifies culture media, having negative effects on cell culture. A stable CHO cell line should be established for the manufacturing process of therapeutic proteins, and the development of stable cell lines is usually based on two expression systems: the dihydrofolate reductase (DHFR) system and the glutamine synthetase (GS) system. Compared to the DHFR system, the GS system produces a reduced level of …

The Statesman, V. 59, I. 28, Stony Brook University

Stony Brook Statesman-Sucolian

v. 59;i. 28

Engineering, Expression Screening, And Production Cell Line Development Of Hetero Ig Molecules Using Charge Pair Mutations, Jennitte Stevens, Guna Kannan, Trent Munro

Cell Culture Engineering XV

In recent years, there has been an increase in therapeutic indications that require bispecific targeting. Bispecific Hetero Ig antibodies that can target two antigens have long been considered as an attractive approach to drive synergistic biologic activity while maintaining the structure and stability of a traditional antibody. However, clinical development of such molecules has been hampered by CMC related challenges relating to product heterogeneity. During the development of a Hetero Ig molecule targeting the Wnt pathway antagonists Dkk-1 and SCL-1, we employed a novel strategy to drive the heterodimerization of IgG antibodies through the addition of charge pair reside mutations …

Predictive Engineering Of Cho Cells Using Systems Biology Models, Nathan Lewis

Cell Culture Engineering XV

Decades of bioprocess optimization have resulted in substantial improvements in recombinant protein production. However, some proteins remain difficult to express, and there is an increasing awareness of the need for improved control of critical quality attributes of recombinant protein drugs. To enable cell engineering efforts to enhance protein production and control product quality, we have enumerated the CHO cell parts through genome sequencing efforts,^1,2 and are now providing context to these parts by reconstructing genome-scale networks of the secretory pathway, glycosylation, and metabolism CHO (Figure 1). Using these models, which account for the activities of more than 2000 genes, …

Accelerated Homology-Directed Targeted Integration Of Transgenes In Cho Cells Via Crispr/Cas9 And Fluorescent Enrichment, Jae Seong Lee, Lise Marie Grav, Gyun Min Lee, Helene Kildegaard

Cell Culture Engineering XV

Development of recombinant CHO cell lines has been hampered by unstable and variable transgene expression caused by random integration. With draft genome of several CHO cell lines and targeted genome editing technologies, rCHO cell line development based on site-specific integration has the potential to overcome the limitations of clonal heterogeneity. In a previous study, we demonstrated efficient and precise targeted gene integration in CHO cells using CRISPR/Cas9 technology and homology-directed repair (HDR) pathway¹⁾. However, it requires a selection process, which limits targeted integration of multiple transgenes at multiple sites due to a limited number of selection markers and …

Mcmannis Family History, Jeremy W. Mcmannis

Your Family in History: HIST 550/700

This is the story of the McMannis Family with its history and stories written by Jeremy McMannis

A Family United Against Huntington’S Disease, Kenneth P. Serbin

At Risk for Huntington's Disease

No abstract provided.

The Dragonflies And Damselflies (Odonata) Of Utah, Alan R. Myrup, Richard W. Baumann

Monographs of the Western North American Naturalist

An updated faunal list containing 94 species of Odonata (60 Anisoptera and 34 Zygoptera) for Utah is presented. Of the 95 Odonata species recorded in past publications as being from Utah, 8 have been removed from the Utah Odonata list, while 7 new state records have been added. Explanations for their removal are provided in the species accounts. The 7 ecoregions found in Utah are briefly described along with their wetland habitats and odonate species. Geographical distribution data by county, drainage, and ecoregion are provided for each species along with information regarding elevation range, flight season, and habitat preferences in …

The Role Of Money And Endorsements In Same-Sex Marriage Ballot Initiatives, Jessica Lorraine Loyet Gracey

Dissertations

This research examines how well certain theories of direct democracy explain outcomes of ballot measures on gay marriage bans. Most theories of direct democracy focus on the types of issues that are quite dissimilar from gay marriage bans. Therefore these theories will likely not do a good job of explaining the results of these elections. In particular I examine the role of campaign spending and elite endorsements in campaigns for gay marriage ban ballot initiatives. In candidate elections, voters commonly use heuristics such as party labels and past performance to help them decide. In most ballot initiative contests voters rely …

Applications Of Nanoporous Gold Monoliths As Substrates For The Capture And Release Of Lectins And Glycoproteins, Allan Jonson Alla

Dissertations

Nanoporous gold (np-Au) monoliths are a free-standing nanostructured material with typical pore dimensions in the tens of nanometers range. The microstructure of np-Au resembles those of macroporous monolithic materials being used in chromatographic separations. The surfaces of np-Au monoliths were modified via flow methods with different ligands to develop affinity substrates for separations. A carbohydrate-modified np-Au monolith was prepared by immobilizing thiolated saccharides and further used to separate lectins. The np-Au monolith surface was also functionalized with self-assembled monolayers (SAMs) of α-lipoic acid (LA) followed by activation of carboxyl terminal groups to create amine reactive esters. Concanavalin A (Con A) …

An Integrated Cell Line Development Platform For Generation Of High Yielding Cho Stable Cell Lines Expressing A Stabilized Trimeric Pre-Fusion Rsv F Recombinant Viral Glycoprotein, Richard Schwartz, Amritha Menon, Mingzhong Chen, Giulia Fabozzi, Althaf Hussain

Cell Culture Engineering XV

Accelerating timelines to deliver stable cell lines with high productivity is challenging, especially for biologics portfolios which include complex recombinant vaccine constructs. The VRC’s current integrated approach to shorten CHO cell line development timeline utilizes host cells pre-adapted to production medium, optimization of the expression vectors, improved pre and post-transfection methodology, automated ClonePix^TM technology and top clone selection and ranking in micro-scale ambr bioreactor technology. This platform was developed with monoclonal antibodies as model proteins. While this integrated platform enabled generation of high yielding clones expressing monoclonal antibodies, it was initially much less successful with a difficult to express …

Generation Of Desirable Cho Cell Factories With Predictive Culture Performance Using Crispr/Cas9-Mediated Genome Engineering, Helene Faustrup

Cell Culture Engineering XV

Chinese hamster ovary (CHO) cells are widely used in the biopharmaceutical industry as a host for the production of complex pharmaceutical proteins. Thus, genome engineering of CHO cells for improved product quality and yield is of great interest. Here, I will demonstrate our latest advances in improving the efficiency of CRISPR/Cas9-mediated genome engineering to generate attractive knockout and knockin CHO cell lines. Analysis of the dynamics and efficiency of the technology will be demonstrated for genes involved in glycosylation and apoptosis. Combined with multiplexing and fluorescent enrichment, application of CRISPR/Cas9 genome editing facilitated disruption of several genes simultaneously and accelerated …

Challenges Developing Biologics For The Prevention And Treatment Of Infectious Diseases Impacting Global Health, Steven Hadley

Cell Culture Engineering XV

Biologics have enjoyed tremendous success as products in high income countries of the world. However the high cost of these products today prevent their use in low income countries and with the notable exception of palivizumab there has been little commercial interest in developing biologics for infectious diseases that are prevalent in these geographies. Recent advances in the study of HIV, malaria, pertussis and Ebola highlight the potential of monoclonal antibodies (mAb) for prevention or treatment of these diseases. The evolution of the industrialized CHO process over the past 30 years to satisfy market demands for mAbs is remarkable. The …

Treatment For Complex Trauma And Substance Abuse For Adult Males In Residential Addiction Treatment, Kristin J. Wilson

Counselor Education Capstones

Research has shown that individuals who have experienced trauma are more likely to abuse substances. Alcohol and other drugs can be used as a means to cope with distressing symptoms of Post-Traumatic Stress Disorder such as intrusive memories, anxiety, and feelings of selfblame and low self-esteem. Complex trauma or early childhood trauma is defined by repeated exposures to traumatic experiences over a prolonged period of time. The perpetrator is often a caregiver or trusted individual and the trauma affects multiple areas of one’s life. The Seeking Safety treatment manual and Trauma-Focused Cognitive-Behavioral Therapy have both been used to treat individuals …