Digital Commons Network^™

Regional Investigation Of A Cyclosporiasis Outbreak Linked To Imported Romaine Lettuce – Nebraska And Iowa, June–August 2013, Bryan F. Buss, M. V. Joshi, C. D. Allensworth, A. Garvey, K. Obbink, S. Mandernach, Thomas J. Safranek

Department of Food Science and Technology: Faculty Publications

A regional, multistate investigation into a June–August 2013 cyclosporiasis outbreak was conducted in Nebraska, Iowa, and neighbouring states. Cases were confirmed on the basis of laboratory and clinical findings. Of 227 cases in Iowa (n = 140) and Nebraska (n=87) residents, 162 (71%) reported dining at chain A/B restaurants – 96% reported house salad consumption. A case-control study identified chain A/B house salad as the most likely vehicle. Traceback was conducted to ascertain production lot codes of bagged salad mix (iceberg and romaine lettuce, red cabbage, and carrots) served as house salad in implicated restaurants. A single production lot code …

Enteric Fever: A Slow Response To An Old Plague, Carlos Franco- Paredes, M. Imran Khan, Esteban Gonzalez- Diaz, Jose I. Santos- Preciado, Alfonso J. Rodriguez Morales, Eduardo Gotuzzo

Centre of Excellence in Women and Child Health

Man is irremediably embedded in nature with complex interactions with all living organisms. Historically, the establishment of contemporary human societies has been influenced by our coexistence with other microorganisms living in highly interconnected habitats and ecologies. As a result, with the progression from unicellular to multicellular life, bacteria have coexisted with humans. In this biological journey, while there are important benefits provided by bacterial guests to the human host living in complex relationships and becoming part of their microbiome, some organisms are able to cause a wide spectrum of diseases. Among the large Enterobacteriaceae family, the genus Salmonella, a …

Will The Eastern Mediterranean Region Be The First To Prevent All Of Folic Acid-Preventable Spina Bifida And Anencephaly?, Godfrey P. Oakley, Vijaya Kancherla

Saudi Medical Journal

No abstract provided.

Low Oxytocin And Melatonin Levels And Their Possible Role In The Diagnosis And Prognosis In Iraqi Autistic Children, Haidar A. Abdulamir, Omar F. Abdul-Rasheed, Emad A. Abdulghani

Saudi Medical Journal

Objectives: To test the possible association between oxytocin and melatonin levels with the severity of social and cognitive dysfunctions, and to study the correlation between these parameters in children with autism. Methods: A case-control study was carried out in the Department of Chemistry and Biochemistry, College of Medicine, Al-Nahrain University, Baghdad, Iraq. The study was performed on 60 male autistic patients recruited from the Pediatric Department of Al-Sader General Hospital, Baghdad, Iraq between November 2014 and April 2015. The levels of oxytocin and melatonin were measured in the serum of these autistic male patients, and categorized as mild, moderate, and …

Association Of Doripenem Resistance With Oxa-Type Carbapenemases In Acinetobacter Baumannii Isolates, Huseyin-Agah Terzi, Ali-Rıza Atasoy, Sadiye-Berna Aykan, Engin Karakece, Gulsah Asık, Ihsan-Hakkı Ciftci

Saudi Medical Journal

Objectives: To evaluate the in vitro activity of doripenem in Acinetobacter baumannii ( A. baumannii ) clinical isolates that possess different OXA-type carbapenemases, and to evaluate the roles of these enzymes in the development of carbapenem resistance. Methods: This retrospective study was conducted with 25 A. baumannii isolates at Sakarya University Training and Research Hospital, Sakarya, Turkey from June to October 2014. Antibiotic susceptibility testing was carried out using the Vitek-2 automated system (bioMérieux, Marcy l’Etoile, France). Minimum inhibitory concentrations (MICs) were determined using Etest strips (bioMérieux, Marcy l’Etoile, France). Quantitative polymerase chain reaction was performed in a Fluorion Instrument …

Mycobacterium Tuberculosis Lineage 4 Comprises Globally Distributed And Geographically Restricted Sublineages, David Stucki, Daniela Brites, Leïla Jeljeli, Mireia Coscolla, Gunturu Revathi

Pathology, East Africa

Generalist and specialist species differ in the breadth of their ecological niches. Little is known about the niche width of obligate human pathogens. Here we analyzed a global collection of Mycobacterium tuberculosis lineage 4 clinical isolates, the most geographically widespread cause of human tuberculosis. We show that lineage 4 comprises globally distributed and geographically restricted sublineages, suggesting a distinction between generalists and specialists. Population genomic analyses showed that, whereas the majority of human T cell epitopes were conserved in all sublineages, the proportion of variable epitopes was higher in generalists. Our data further support a European origin for the most …

The Differentiation And Protective Function Of Cytolytic Cd4 T Cells In Influenza Infection, Deborah M. Brown, Anna T. Lampe, Aspen M. Workman

School of Biological Sciences: Faculty Publications

CD4 T cells that recognize peptide antigen in the context of class II MHC can differentiate into various subsets that are characterized by their helper functions. However, increasing evidence indicates that CD4 cells with direct cytolytic activity (CD4 CTL) play a role in chronic as well as acute infections, such as influenza A virus (IAV) infection. In the last couple of decades, techniques to measure the frequency and activity of these cytolytic cells has demonstrated their abundance in infections, such as human immunodeficiency virus, mouse pox, murine gamma herpes virus, cytomegalovirus, Epstein–Barr virus, and influenza among others. We now appreciate …

Diseases Of Trees In The Great Plains, Aaron D. Bergdahl, Alison Hill, Gerard C. Adams, Rachel A. Allison, John A. Appel, John Ball, James T. Blodgett, Kelly S. Burns, Whitney Cranshaw, Kelsey L. Dunnell, Mark L. Gleason, Mark O. Harrell, Thomas C. Harrington, Marcus B. Jackson, William R. Jacobi, Jennifer Juzwik, Megan M. Kennwlly, Les Koch, Jared M. Leboldus, Phillip A. Mason, Douglas L. Mcnew, Judith O'Mara, Hayley M. Nelson, Nicole Opbroek, Michael E. Ostry, Scott C. Redlin, Glen R. Stanosz, Laurie J. Stepanek, Ned Tisserat, Timothy C. Todd, James A. Walla, James J. Worrall, Simeon Wright

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Hosts, distribution, symptoms and signs, disease cycle, and management strategies are described for 84 hardwood and 32 conifer diseases in 56 chapters. Color illustrations are provided to aid in accurate diagnosis. A glossary of technical terms and indexes to hosts and pathogens also are included.

Scope

The purpose of this handbook is to assist users in diagnosing tree diseases and reducing their impact. Included are the most damaging diseases, along with some that are the subject of frequent inquiry although of minor importance. The diseases included were those encountered primarily on trees in conservation plantings such as windbreaks, farmstead plantings, …

Current Rural Drug Use In The Us Midwest, Kirk Dombrowski, Devan M. Crawford, Bilal Khan, Kimberly A. Tyler

Department of Sociology: Faculty Publications

The nature and challenge of illicit drug use in the United States continues to change rapidly, evolving in reaction to myriad social, economic, and local forces. While the use of illicit drugs affects every region of the country, most of our current information about drug use comes from large urban areas. Data on rural drug use and its harms justify greater attention. Record overdose rates, unexpected outbreaks of HIV, and a dearth of treatment facilities point to a rapidly worsening health situation. While health sciences have made considerable progress in understanding the etiology of drug use and uncovering the link …

Chapter 8 Keeping Wildlife Out Of Your Food: Mitigation And Control Strategies To Reduce The Transmission Risk Of Food-Borne Pathogens, Alan B. Franklin, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

In this chapter, we provide a general framework for developing strategies to mitigate the contamination of agricultural operations with pathogens carried by wildlife. As part of this framework, we present adaptive management as a viable approach to developing these strategies to reduce the uncertainty over time as to whether management methods are being effective. We provide the general steps to developing an adaptive management strategies as well as generic mitigation methods that can be applied to agricultural operations as part of an adaptive management strategy.

Fishing Diseased Abalone To Promote Yield And Conservation, Tal Ben-Horin, Kevin D. Lafferty, Gorka Bidegain, Hunter S. Lenihan

United States Geological Survey: Staff Publications

Past theoretical models suggest fishing disease-impacted stocks can reduce parasite transmission, but this is a good management strategy only when the exploitation required to reduce transmission does not overfish the stock. We applied this concept to a red abalone fishery so impacted by an infectious disease (withering syndrome) that stock densities plummeted and managers closed the fishery. In addition to the non-selective fishing strategy considered by past disease- fishing models, we modelled targeting (culling) infected individuals,which is plausible in red abalone because modern diagnostic tools can determine infection without harming landed abalone and the diagnostic cost is minor relative to …

Disease-Emergence Dynamics And Control In A Socially-Structured Wildlife Species, Kim M. Pepin, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

Once a pathogen is introduced in a population, key factors governing rate of spread include contact structure, supply of susceptible individuals and pathogen life-history. We examined the interplay of these factors on emergence dynamics and efficacy of disease prevention and response. We contrasted transmission dynamics of livestock viruses with different life-histories in hypothetical populations of feral swine with different contact structures (homogenous, metapopulation, spatial and network). Persistence probability was near 0 for the FMDV-like case under a wide range of parameter values and contact structures, while persistence was probable for the CSFV-like case. There were no sets of conditions where …

High Rates Of Detection Of Clade 2.3.4.4 Highly Pathogenic Avian Influenza H5 Viruses In Wild Birds In The Pacific Northwest During The Winter Of 2014–15, Hon S. Ip, Robert J. Dusek, Barbara Bodenstein, Mia Kim Torchetti, Paul Debruyn, Kristin Mansfield, Thomas J. Deliberto, Jonathan M. Sleeman

United States Department of Agriculture Wildlife Services: Staff Publications

SUMMARY. In 2014, clade 2.3.4.4 H5N8 highly pathogenic avian influenza (HPAI) viruses spread across the Republic of Korea and ultimately were reported in China, Japan, Russia, and Europe. Mortality associated with a reassortant HPAI H5N2 virus was detected in poultry farms in western Canada at the end of November. The same strain (with identical genetic structure) was then detected in free-living wild birds that had died prior to December 8, 2014, of unrelated causes in Whatcom County, Washington, U. S. A., in an area contiguous with the index Canadian location. A gyrfalcon (Falco rusticolus) that had hunted and …

Highly Pathogenic Avian Influenza Viruses And Generation Of Novel Reassortants, United States, 2014–2015, Dong-Hun Lee, Justin Bahl, Mia Kim Torchetti, Mary Lea Killian, Hon S. Ip, Thomas J. Deliberto, David E. Swayne

United States Department of Agriculture Wildlife Services: Staff Publications

Asian highly pathogenic avian influenza A(H5N8) viruses spread into North America in 2014 during autumn bird migration. Complete genome sequencing and phylogenetic analysis of 32 H5 viruses identified novel H5N1, H5N2, and H5N8 viruses that emerged in late 2014 through reassortment with North American low-pathogenicity avian influenza viruses.

Ecosystem Services Provided By Avian Scavengers, Travis L. Devault, James C. Beasley, Zachary H. Olson, Marcos Moleón, Martina Carrete, Antoni Margalida, José Antonio Sánchez-Zapata

United States Department of Agriculture Wildlife Services: Staff Publications

Food webs developed under classical theoretical models often depict simplistic interactions among trophic levels linked by predation (Hairston et al. 1960). As a result, extensive research efforts have been devoted to studying predator-prey interactions, often ignoring the contribution of scavenging in food-web dynamics. However, recent advancements in food-web theory have recognized the widespread and critical role that scavenging plays in stabilizing food webs in ecosystems throughout the world, thus suggesting that previous models may have greatly underestimated the importance of scavenging in food web research ( Wilson and Wolkovich 2011; Barton et al. 2013). Such disregard for the importance of …

Role For Migratory Wild Birds In The Global Spread Of Avian Influenza H5n8

United States Department of Agriculture Wildlife Services: Staff Publications

Avian influenza viruses affect both poultry production and public health. A subtype H5N8 (clade 2.3.4.4) virus, following an outbreak in poultry in South Korea in January 2014, rapidly spread worldwide in 2014–2015. Our analysis of H5N8 viral sequences, epidemiological investigations, waterfowl migration, and poultry trade showed that long-distance migratory birds can play a major role in the global spread of avian influenza viruses. Further, we found that the hemagglutinin of clade 2.3.4.4 virus was remarkably promiscuous, creating reassortants with multiple neuraminidase subtypes. Improving our understanding of the circumpolar circulation of avian influenza viruses in migratory waterfowl will help to provide …

Antigenic Characterization Of H3 Subtypes Of Avian Influenza A Viruses From North America, Elizabeth Bailey, Li-Ping Long, Nan Zhao, Jeffrey S. Hall, John A. Baroch, Jacqueline M. Nolting, Lucy Senter, Frederick L. Cunningham, G. Todd Pharr, Larry Hanson, Richard Slemons, Thomas J. Deliberto, Xiu-Feng Wan

United States Department of Agriculture Wildlife Services: Staff Publications

Besides humans, H3 subtypes of influenza A viruses (IAVs) can infect various animal hosts, including avian, swine, equine, canine, and sea mammal species. These H3 viruses are both antigenically and genetically diverse. Here, we characterized the antigenic diversity of contemporary H3 avian IAVs recovered from migratory birds in North America. Hemagglutination inhibition (HI) assays were performed on 37 H3 isolates of avian IAVs recovered from 2007 to 2011 using generated reference chicken sera. These isolates were recovered from samples taken in the Atlantic, Mississippi, Central, and Pacific waterfowl migration flyways. Antisera to all the tested H3 isolates cross-reacted with each …

Widespread Detection Of Antibodies To Eastern Equine Encephalitis, West Nile, St. Louis Encephalitis, And Turlock Viruses In Various Species Of Wild Birds From Across The United States, Kerri Pedersen, David R. Marks, Eryu Wang, Gillian Eastwood, Scott C. Weaver, Samuel M. Goldstein, David R. Sinnett, Thomas J. Deliberto

United States Department of Agriculture Wildlife Services: Staff Publications

Wild birds serve as amplifying hosts for many arboviruses, and are thought to be responsible for introducing these viruses into new areas during migration as well as reintroducing them to places where winter temperatures disrupt mosquito-borne transmission. To learn more about four mosquito-borne arboviruses of concern to human or animal health, we tested sera from 997 wild birds of 54 species and 17 families across 44 states of the United States collected from January 1, 2013, through September 30, 2013. Samples were tested for antibody against eastern equine encephalitis, St. Louis encephalitis, West Nile, and Turlock viruses using plaque reduction …

Transcriptional Profiling Of Ileocecal Valve Of Holstein Dairy Cows Infected With Mycobacterium Avium Subsp. Paratuberculosis, Randy J. Hempel, J. P. Bannantine, Judith R. Stabel

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Johne’s disease is a chronic infection of the small intestine caused by Mycobacterium avium subspecies paratuberculosis (MAP), an intracellular bacterium. The events of pathogen survival within the host cell(s), chronic inflammation and the progression from asymptomatic subclinical stage to an advanced clinical stage of infection, are poorly understood. This study examines gene expression in the ileocecal valve (ICV) of Holstein dairy cows at different stages of MAP infection. The ICV is known to be a primary site of MAP colonization and pro-vides an ideal location to identify genes that are relevant to the progression of this disease. RNA was prepared …

Japanese Encephalitis: Assessing Disease Risk Due To Landscape Factors At Multiple Scales, Julia E. Metelka

Theses and Dissertations (Comprehensive)

Japanese Encephalitis is a mosquito-borne disease and is the leading cause of viral encephalitis in Asia. In many Asian countries, the geographical distribution of JE is dependent on a variety of human-environment interactions that can be conceptualized as a complex social-ecological system. The JE transmission cycle is influenced by a few primary human-landscape factors; the abundance and the spatial configuration of rice paddy fields (which provide habitat for the vector), the distribution of pig farms (which position the virus' amplifying host), and the location of a susceptible human population. Our models integrate population dynamics, landscape characteristics, and weather variables that …

Tick-, Mosquito-, And Rodent-Bourne Parasite Sampling Designs For The National Ecological Observatory Network, Yuri Springer, David Hoekman, Pieter T.J. Johnson, Paul A. Duffy, Rebecca A. Hufft, Holly D. Gaff

Biological Sciences Faculty Publications

Parasites and pathogens are increasingly recognized as significant drivers of ecological and evolutionary change in natural ecosystems. Concurrently, transmission of infectious agents among human, livestock, and wildlife populations represents a growing threat to veterinary and human health. In light of these trends and the scarcity of long-term time series data on infection rates among vectors and reservoirs, the National Ecological Observatory Network (NEON) will collect measurements and samples of a suite of tick-, mosquito-, and rodent-borne parasites through a continental-scale surveillance program. Here, we describe the sampling designs for these efforts, highlighting sampling priorities, field and analytical methods, and the …

Anaplasma Marginale Actively Modulates Vacuolar Maturation During Intracellular Infection Of Its Tick Vector, Dermacentor Andersoni, Foregivemore Magunda, Chelsea Wright Thompson, David A. Schneider, Susan M. Noh

Biological Sciences Faculty Publications

ABSTRACT Tick-borne transmission of bacterial pathogens in the order Rickettsiales is responsible for diverse infectious diseases, many of them severe, in humans and animals. Transmission dynamics differ among these pathogens and are reflected in the pathogen-vector interaction. Anaplasma marginale has been shown to establish and maintain infectivity within Dermacentor spp. for weeks to months while escaping the complex network of vacuolar peptidases that are responsible for digestion ofthe tick blood meal. How this prolonged maintenance of infectivity in a potentially hostile environment is achieved has been unknown. Using the natural vector Dermacentor andersoni, we demonstrated that A. marginale …

Investigating Cholera Using An Sir Model With Age-Class Structure And Optimal Control, K. Renee Fister, Holly Gaff, Elsa Schaefer, Glenna Buford, Bryce C. Norris

Biological Sciences Faculty Publications

The use of systems of differential equations in mathematical modeling in conjunction with epidemiology continues to be an area of focused research. This paper briefly acquaints readers with epidemiology, cholera, and the need for effective control strategies; discusses cholera dynamics through a variation on the SIR epidemiological model in which two separate age classes exist in a population; finds the numeric value for R₀ to be approximately 1.54 using estimated parameters for Bangladesh; and employs an optimal control resulting in a suggestion that a protection control be implemented at the end of the monsoon season.

Tick Genome Assembled: New Opportunities For Research On Tick-Host-Pathogen Interactions, Jose De La Fuente, Robert M. Waterhouse, Daniel E. Sonenshine, R. Michael Roe, Jose M. Ribeiro, David B. Sattelle, Catherine A. Hill

Biological Sciences Faculty Publications

As tick-borne diseases are on the rise, an international effort resulted in the sequence and assembly of the first genome of a tick vector. This result promotes research on comparative, functional and evolutionary genomics and the study of tick-host-pathogen interactions to improve human, animal and ecosystem health on a global scale.

Paleoparasitological Evidence Of Pinworm (Enterobius Vermicularis) Infection In A Female Adolescent Residing In Ancient Tehran (Iran) 7000 Years Ago, Niloofar Paknazhad, Gholamreza Mowlavi, Jean Dupouy Camet, Mohammad Esmaeili Jelodar, Iraj Mobedi, Mahsasadat Makki, Eshrat Beigom Kia, Mostafa Rezaeian, Mehdi Mohebali, Siamak Sarlak, Faezeh Najafi

Harold W. Manter Laboratory of Parasitology: Library Materials

Background: The Molavi street archeological site south of Tehran, Iran accidentally provided a unique opportunity for paleoparasitological studies in Iran. A female skeleton was unearthed and evaluated to be 7,000 years old. Soil samples were collected around the pelvic and sacrum bones.

Findings: Careful microscopic investigation of rehydrated soil samples revealed the presence of one Enterobius vermicularis egg attached to the skeleton sacral region.

Conclusion: The present finding likely represents the oldest evidence of a human pinworm infection in Asia.

Comparative Memory/Behavioral Symptoms Of Alzheimer's Disease: Eoad Vs. Load, Marcia Gale Roberson

Walden Dissertations and Doctoral Studies

Alzheimer's disease (AD) is a fatal disorder with no apparent cure. Early-onset AD (EOAD) occurs in individuals before the age of 65, and late-onset AD (LOAD) occurs in individuals age 65 and older. Past studies have proven that AD is fatal among Americans age 65 and older. The disease is characterized by impairments in memory and executive function as well as other cognitive and behavioral problems. The research questions addressed by this sequential, mixed-method study compared EOAD and LOAD by exploring common behavioral/cognitive symptoms and stage levels of AD. Research participants were recruited from the Alzheimer's Association who were members …

Managing Hiv/Aids Outreach Strategies In The Black Church: A Case Study, Angela Gail Hicks-Bennett

Walden Dissertations and Doctoral Studies

The problem under investigation is that there is little research about how church leaders develop and design HIV/AIDS education and support programs in selected counties of New York State. The gap in the literature is that there is little known about how church leaders support the educational process for the HIV/AIDS Black community in New York state. The theory of normative decision making was the primary conceptual framework for this research. The purpose of this qualitative exploratory multi-case study was to discover how church leaders managed their HIV/AIDS outreach program strategies. The overarching question asked what programs and education do …

Characteristics Of Adult Icu Patients With Device Associated Nosocomial Infections, Doramarie Arocha

Walden Dissertations and Doctoral Studies

Nosocomial infections are a cause of concern for hospital patients and the incidence rates of these infections are greater in intensive care units (ICUs) due to the invasive nature of treatments, additional risk factors and comorbidities, and therapies used. Invasive devices, such as vascular central lines, Foley catheters, and mechanical ventilators pose a risk for critically ill patients in the ICUs to develop device-related, healthcare-associated infections (HAI). The purpose of this study was to describe the epidemiological characteristics of patients who developed device-related HAIs within 3 ICU units (medical-surgical, cardiovascular, and neurosurgical) of an academic medical facility. The ecosocial theory …

Barriers To Colorectal Cancer Screening In People Obtaining Care From Community Mental Health Agencies, Kelly L. Gardiner

Walden Dissertations and Doctoral Studies

Barriers to Colorectal Cancer Screening in People Obtaining Care

From Community Mental Health Agencies

by

Kelly Gardiner

MSN, Wayne State University, 1997

BSN, Wayne State University, 1988

Dissertation Submitted in Fulfillment

of the Requirements for the Degree of

Doctor of Philosophy

Public Health

Walden University

August 2016

Despite being highly treatable with early intervention and preventative screenings, the overall mortality rate of colorectal cancer is substantially higher in participants with a preexisting mental disorder. Variables affecting the likelihood of completing screening for those with mental illnesses were unknown in people who obtain services from a Community Mental Health agency. Using …

Chronic Disease Management Of The Uninsured Patient At Ohio Free Clinics, James Benedict

Walden Dissertations and Doctoral Studies

Management of chronic disease requires a different service delivery model from that of acute illness. The uninsured population experience poorer health status and increased incidence of chronic disease than do the insured population. The purpose of this study was to identify the supports and barriers present in providing chronic disease management to patients at Ohio free clinics. Wagner's theory of chronic disease management served as the theoretical lens. The sequential, exploratory mixed methods study collected data from 13 free clinics belonging to the Ohio Association of Free Clinics (OAFC). Quantitative questions focused on processes in clinics with high and low …