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Photoacoustic Microscopy And Photoacoustic Computed Tomography Using High-Frequency Linear Array Ultrasonic Transducers, Guo Li

McKelvey School of Engineering Graduate Student Theses & Dissertations

Photoacoustic tomography (PAT) is a highly promising imaging technology which forms images by detecting the induced pressure waves resulting from pulsed light absorption in biological tissues. Because the excitation source is light, PAT is a very safe, non-ionizing, and non-carcinogenic imaging technology. In biomedicine, PAT has the unique advantage of probing endogenous optical absorbers at different length scales with 100% relative sensitivity. With such scalability, PAT can image anatomical, functional, metabolic, molecular, and genetic contrasts of vasculature, hemodynamics, oxygen metabolism, biomarkers, and gene expression. Among several implementations of PAT, optical-resolution photoacoustic microscopy (OR-PAM) and photoacoustic computed tomography (PACT) are two …

Using Fluorescence – Polarization Endoscopy In Detection Of Precancerous And Cancerous Lesions In Colon And Pancreatic Cancer, Tauseef Charanya

McKelvey School of Engineering Graduate Student Theses & Dissertations

Colitis-associated cancer (CAC) arises from premalignant flat lesions of the colon, which are difficult to detect with current endoscopic screening approaches. We have developed a complementary fluorescence and polarization reporting strategy that combines the unique biochemical and physical properties of dysplasia and cancer for real time detection of these lesions. Utilizing a new thermoresponsive sol-gel formulation with targeted molecular probe allowed topical application and detection of precancerous and cancerous lesions during endoscopy. Incorporation of nanowire-filtered polarization imaging into NIR fluorescence endoscopy served as a validation strategy prior to obtaining biopsies.

In order to reduce repeat surgeries arising from incomplete tumor …

Online Modeling And Tuning Of Parallel Stream Processing Systems, Jonathan Curtis Beard

McKelvey School of Engineering Graduate Student Theses & Dissertations

Writing performant computer programs is hard. Code for high performance applications is profiled, tweaked, and re-factored for months specifically for the hardware for which it is to run. Consumer application code doesn't get the benefit of endless massaging that benefits high performance code, even though heterogeneous processor environments are beginning to resemble those in more performance oriented arenas. This thesis offers a path to performant, parallel code (through stream processing) which is tuned online and automatically adapts to the environment it is given. This approach has the potential to reduce the tuning costs associated with high performance code and brings …

Synthesis Of Clickable Poly(Ethylene Glycol) Derivatives For Fabrication Of Modular Microsphere-Based Scaffolds To Promote Vascularization, Peter Nguyen

McKelvey School of Engineering Graduate Student Theses & Dissertations

Vascularization plays an important role in supporting transplanted tissues and cells in tissue engineering applications. Most tissues require access to blood vessels for the delivery of oxygen and nutrients, as well as the removal of carbon dioxide and cellular waste products. Without an adequate blood supply, cells within tissue-engineered constructs and scaffolds lose viability and fail to perform their intended functions. The goal of this dissertation was to design scaffolds that can promote vascularization of biomaterial implants for biomedical applications. In order to accomplish this goal, clickable poly(ethylene glycol) (PEG) derivatives were synthesized in order to fabricate modular microsphere-based scaffolds …

Directed Differentiation And Characterization Of Spinal V3 Interneurons From Mouse Embryonic Stem Cells, Hao Xu

McKelvey School of Engineering Graduate Student Theses & Dissertations

Neuronal populations involved in locomotion controlling central pattern generators within the spinal cord hold great potential for spinal cord injury therapy. Spontaneous recovery in rodent models suggests that a population that can reorganize around an injury site could be useful for functional recovery therapeutics after spinal cord injury. The glutamatergic, commissural, long-extending V3 interneurons shown to balance locomotor rhythm regularity and robustness within central pattern generators in vivo are both an ideal population for spinal cord injury therapeutics and a vital population to study as a part of locomotor circuitry. Unfortunately, due to the scarcity of these cells in the …

Observation And Prediction Of Three-Dimensional Morphology At A High-Energy Macrotidal Beach, Christopher Stokes, Mark Davidson, Paul Russell

School of Biological and Marine Sciences

No abstract provided.

Estimated Mouse Population At The Pennypack Ecological Restoration Trust Vs. Reported Incidence Of Lyme Disease In Pennsylvania, Erika Clark

The Compass

The purpose of this research paper is to determine if the mouse population at the PennyPack Trust is correlated with occurrence of Lyme disease state wide.

Mark-recapture studies of white footed mice, Peromyscus Leucopus, were held at the PennyPack Ecological Restoration Trust in Huntingdon, Pennsylvania in order to estimate the population sizes over six years. The common method of ear tagging has been shown not to interfere with the daily activities of mice, or increase their chances of mortality.

White-footed mice are primary hosts of the black-legged tick, Ixodes scapularis, which is the vector of Borrelia burgdorferi, the bacteria that …

Proceedings Of The 2015 Berry Summer Thesis Institute, University Of Dayton. University Honors Program

Proceedings of the Berry Summer Thesis Institute (2013-Present)

Thanks to a gift from the Berry Family Foundation and the Berry family, the University Honors Program launched the Berry Summer Thesis Institute in 2012. The institute introduces students in the University Honors Program to intensive research, scholarship opportunities and professional development. Each student pursues a 12-week summer thesis research project under the guidance of a UD faculty mentor. This contains the product of the students' research.

Public Services Annual Report 2014-2015, Deborah Mongeau

Public Services Reports

Annual report of the Public Services Department at the University of Rhode Island Libraries.

Nosh-Sulindac (Avt-18a) Is A Novel Nitric Oxide- And Hydrogen Sulfide-Releasing Hybrid That Is Gastrointestinal Safe And Has Potent Anti-Inflammatory, Analgesic, Antipyretic, Anti-Platelet, And Anti-Cancer Properties, Khosrow Kashfi, Mitali Chattopadhyay, Ravinder Kodela

Publications and Research

Sulindac is chemopreventive and has utility in patients with familial adenomatous polyposis; however, side effects preclude its long-term use. NOSH-sulindac (AVT-18A) releases nitric oxide and hydrogen sulfide, was designed to be a safer alternative. Here we compare the gastrointestinal safety, anti-inflammatory, analgesic, anti-pyretic, anti-platelet, and anti-cancer properties of sulindac and NOSH-sulindac administered orally to rats at equimolar doses. Gastrointestinal safety: 6 h post-administration, number/size of hemorrhagic lesions in stomachs were counted. Tissue samples were frozen for PGE2, SOD, and MDA determination. Anti-inflammatory: 1 h after drug administration, the volume of carrageenan-induced rat paw edemas was measured for 5 h. Anti-pyretic: …

From Orphans To Families In Crisis: Parental Rights Matters In Maine Probate Courts, Deirdre M. Smith

Faculty Publications

This Article examines the sources of the contemporary problems associated with the adjudication of parental rights matters in Maine’s probate courts and identifies specific reforms to address both the structural and substantive law problems. The Article first reviews the development of Maine’s probate courts and their jurisdiction over parental rights matters. It traces the expansion of jurisdiction over children and families from a limited role incidental to the administration of a decedent’s estate to the current scope: a range of matters that may result in the limitation, suspension, or termination of the rights of living parents. Maine probate courts now …

An Examination Of Net Primary Production In Southern Appalachian Wetlands, Mike Maguigan

Theses and Dissertations

Southern Appalachian wetlands have yet to be studied in terms of net primary production (NPP), thus few studies have been conducted to examine what environmental factors have relationships with NPP. To that end, this research investigates several facets of southern Appalachian wetland production. The research was divided into three studies. The first study was conducted to answer the question of what environmental factors have relationships with NPP. It appears that stream discharge and annual precipitation had the strongest relationships with NPP (r = 0.91, p <0.05 and r = 0.81, p <0.05, respectively), yet both factors showed multicolinearity (r = 0.97, p <0.05). The strong relationships between hydrologic factors and NPP is similar to montane wetlands in the western United States. The second study was conducted to examine the relationship between water chemistry and NPP. Calcium (Ca), Magnesium (Mg), and pH were examined in order to determine if any of the aforementioned factors had a relationship with NPP. Neither Ca (r = -0.34, p = 0.0835) nor Mg (r =-0.38, p = 0.0535) had strong relationships with NPP, though pH (r = -0.66, p <0.05) had a strong negative relationship with NPP. The acidity of the stream water, driven by the acid rain in the southern Appalachians, creates enhanced conditions for wetland plants to grow. The third study was conducted to establish which vegetation index was best for estimating NPP from proximally and remotely sensed data. The findings suggest that VARIRed Edge was best for examining NPP at the in situ level, NDVI was best for examining NPP at the airborne level, and the DVI was the best for examining NPP at the satellite level. NPP in southern Appalachian wetlands is driven by the chemistry, specifically the pH, of stream discharge and annual precipitation and can be monitored by NDVI using NAIP data or DVI using Landsat data. The examination of NPP in southern Appalachians in response to environmental factors and water chemistry along with the examination of vegetation indices at three levels of platforms will help to monitor and manage these rare and unique ecosystems in the future.

Report On 2014-2015 Compliance With Continuous Quality Improvement Legislation, University Of Northern Iowa

Institutional Effectiveness & Planning Documents

A report provided to the Iowa Board of Regents regarding continuous improvement processes in courses at the University of Northern Iowa.

Transcriptional And Post-Transcriptional Regulation Of Hepcidin And Iron Metabolism By Lipid Signaling In The Liver, Sizhao Lu

Theses & Dissertations

Although iron is required for essential biological processes, excess iron is detrimental due to oxidative damage induced by iron-mediated Fenton reactions, which promote tissue injury. Cellular iron uptake, transport and storage must therefore be tightly regulated. This task is accomplished mainly through hepcidin, the key iron-regulatory hormone. Hepcidin is synthesized primarily in hepatocytes as a circulatory antimicrobial peptide. It controls iron metabolism by inhibiting iron absorption from the duodenum and iron release from reticuloendothelial macrophages. Besides synthesizing hepcidin, the liver plays an important role in maintaining iron homeostasis by serving as the main storage organ for excess iron. Patients with …

Variables In The Introduction Of The Species, Streptocephalus Sealii, Spinytail Fairy Shrimp Into A Local Seasonal Wetland, Jane Anne Porterfield

School of Education and Leadership Student Capstone Theses and Dissertations

The research question addressed in this capstone is what factors play a role in the introduction of a wingless aquatic invertebrate to a recently restored mitigation wetland. The motivation for this capstone was the discovery of a fairy shrimp, Streptocephalus sealii in a Rosemount, Minnesota wetland in 2012. This organism was previously recorded from two other sites in Minnesota at least 35 miles from this location. Its appearance in a mitigation wetland generated questions as to whether restoration materials brought in the species or the mitigation created the environment in which the organism could thrive. This study investigates both of …

Assessing The Impact Of Restrictiveness And Placement Type On Transition-Related Outcomes For Youth With And Without Disabilities Aging Out Of Foster Care, Jessica Danielle Schmidt

Dissertations and Theses

Nearly 23,000 youth age out of the foster care system between the ages of 18 and 21 each year in a transition fraught with challenges and barriers. These young people often lack developmentally appropriate experiences and exposure to necessary knowledge, role modeling, skill building, and long-term social support to promote positive transitions to adulthood while in foster care. As a result, young people who exit care face an array of poor adult outcomes. Nearly 60% of transition-aged foster youth experience a disability, and as such, face compounded challenges exiting foster care. While the examination of young adult outcomes for youth …

Effect Of Chemical Pocket Disinfection As An Adjunct To Non-Surgical Maintenance Therapy Of Inflamed Periodontal Pockets, Chad Riggs

Theses & Dissertations

Purpose: Scaling and root planing with adjunctive chemical pocket disinfection (SRP+C) utilizing sodium hypochlorite solution has been used to treat inflamed pockets of periodontal maintenance patients for many years, without evidence of its benefits. The primary objective was to determine if SRP+C is more effective than scaling and root planing alone (SRP) in improving clinical outcomes. The secondary objective was to compare the effect of SRP+C and SRP on pro-inflammatory IL-1β, anti-inflammatory IL-1ra, and anti-inflammatory index (IL-1ra/IL-1β ratio) found in gingival crevicular fluid (GCF).

Materials and Methods: Pockets (≥ 5 mm and BOP) of 31 maintenance patients were included. Test …

Use Of Smartphones To Capture Measures Of Functional Status In Frail And Non-Frail Community Dwelling Older Adults, Cassia R. Hanton

Theses & Dissertations

Numerous important health outcomes directly relate to one's ability to maintain normal gait speed. The purpose of this study is to employ ubiquitous smartphone technology, using algorithms developed and validated by our lab in a controlled setting, to continuously and noninvasively measure aspects of subject health status, including step counts, gait speed, and activity level, in a naturalistic community setting. A total of 33 ambulatory, independently dwelling older adults were recruited from Nebraska Medicine, including 22 healthy control and 11 frail individuals. Clinical performance measurements of frailty (4MW, TUG, F8W) and validated survey responses (LLFDI, SAFFE, PROMIS) were compared to …

Targeted Magnetite Tissue Delivery For Antiretroviral Pharmacokinetics, Tianyuzi Li

Theses & Dissertations

Pharmacokinetics and pharmacodynamics studies are required for bench to bedside translation of any new drug, formulation or device. Multifunctional magnetite nanocarriers enable magnetic resonance imaging tracking of nanomaterial encased antiretroviral drugs serving to improve the effectiveness of formulation developments. Targeting ligands used to deliver nanoparticles to HIV harboring cells can be tested using multifunctional magnetite nanocarriers. To this end, two types of magnetite nanocarriers were developed. These included small magnetite antiretroviral therapy particles. The second were ALN-PEG coated magnetite particles for testing macrophages targeting ligands. Overall, these works should serve to speed the development of long acting nanoformulated ART to …

Improving Antibiotic Resistant Infection Transmission Situational Awareness In Enclosed Facilities With A Novel Graphical User Interface For Tactical Biosurveillance, Valeriya V. Kettelhut

Theses & Dissertations

Serious challenges associated with antibiotic resistant infections (ABRIs) force healthcare practitioners (HCP) to seek innovative approaches that will slow the emergence of new ABRIs and prevent their spread. It was realized that traditional approaches to infection prevention based on education, retrospective reports, and biosurveillance often fail to ensure reliable compliance with infection prevention guidelines and real-time problem solving. The objective of this original research was to develop and test the conceptual design of a situational awareness (SA)-oriented information system for coping with healthcare-associated infection transmission.

Constantly changing patterns in spatial distribution of patients, prevalence of infectious cases, clustering of contacts, …

Development Of Polymer Peptide Conjugates For Enhanced Pancreatic Cancer Imaging, Wen Shi

Theses & Dissertations

Pancreatic ductal adenocarcinoma (PDAC) is the fourth leading cause of cancer-related deaths in the US with very poor prognosis. All clinically available biomarkers and diagnostic tools either fail to detect early stage PDAC or suffer from low specificity and sensitivity. There is an urgent need for diagnostic agents with greater efficacy for PDAC detection and staging. Nanomaterials such as N-(2-Hydroxypropyl) methacrylamide (HPMA) copolymers can effectively target tumors, and offer novel opportunities for the development of effective diagnostic agents for cancer. However, a major problem of many nanomaterials-based diagnostics is their opsonization and sequestration by the mononuclear phagocyte system (MPS), leading …

The Role Of Glutaminase 1 In Hiv-1 Associated Neurocognitive Disorders And In Brain Development, Yi Wang

Theses & Dissertations

Glutaminase is the enzyme that converts glutamine into glutamate, which serves as a key excitatory neurotransmitter and one of the energy providers for cellular metabolism. Glutamate is essential for proper brain functioning but at excess levels, it is neurotoxic and has a key role in the pathogenesis of various neurodegenerative diseases, including HIV-1 associated neurocognitive disorders (HAND). However, the detailed mechanism of glutamate-mediated neurotoxicity remains unclear. In part I, we identified the regulation of glutaminase 1 (GLS1) in the central nervous system (CNS) of HAND animal models including HIV-Tat transgenic (Tg) mouse and HIVE-SCID mouse, since GLS1 is the dominant …

Protecting Ecosystems, Culture, And Human Rights In Chile Through Indigenous And Community-Conserved Territories And Areas, William G. Crowley

Capstone Collection

In environmental conservation circles around the world, the contributions of indigenous peoples and local communities to the sustainable maintenance of ecosystems and natural resources are being given increased attention. Whether for cultural, spiritual, economic, or other purposes, the use of traditional and local knowledge of habitat and resource management is slowly making its way into the modern environmental movement, and is being incorporated into the dominant conservation paradigms. These managed areas, known as Indigenous and Community-Conserved Territories and Areas, or ICCAs, are defined by the International Union for Conservation of Nature as “natural and/or modified ecosystems containing significant biodiversity …

Factors Influencing Honey Bee Abundance Across Agricultural Landscapes In The Midsouth, Daniel Adam Whalen

Theses and Dissertations

Populations of honey bees have declined worldwide in recent years. One suspected cause is the widespread use of pesticides in agriculture. Experiments were conducted to examine potential exposure routes of pesticides to honey bees in the Midsouth. Neonicotinoid seed treatment compounds were studied to determine the rate at which they drift during planting and the rate at which they diminish in crop tissue during crop development. Honey bee foraging activity in Midsouth crops was observed to determine when and at what densities foraging honey bees could be active during pesticide applications. This project was designed to aid in understanding the …

Allelopathy In The Non-Native Macrophyte, Myriophyllum Spicatum And Its Influence On Trophic Dynamics In Aquatic Systems, Daniel J. Sullivan

Theses and Dissertations

Non-native macrophytes structurally impact aquatic assemblages, yet little is known regarding how they influence energy pathways in freshwater ecosystems. Allelopathy in Eurasian watermilfoil- Myriophyllum spicatum has been shown to target basal epiphytic organisms resulting in differences in assemblage structure of colonizing epiphyton between M. spicatum and native M. sibiricum. I conducted a growth chamber experiment to investigate the hypothesis that differences in assemblage structure of colonizing epiphyton between these two macrophytes influence trophic dynamics within aquatic systems. My data suggest M. spicatum produces higher concentrations of allelochemicals, resulting in a more diverse epiphytic assemblage compared to M. sibiricum. This could …

Diagnostic Utility Of Who Defined Sepsis Syndromic Criteria With Procalcitonin In Identification Of Sepsis In Children With Suspected Infections, Samuel Owuor Akech

Master of Medicine in Paediatrics and Child Health

Title: Diagnostic Utility of WHO Defined Sepsis syndromic Criteria with Procalcitonin in Identification of Sepsis in Children with Suspected Infections Background: Bacterial infections are a major cause of morbidity and mortality in children but accurate and timely diagnosis remains a challenge to the frontline clinicians. Cultures of sterile bodily specimens are considered the gold standard but are insensitive and results are delayed. Procalcitonin (PCT) has recently emerged as a biomarker with high sensitivity and specificity making it superior to culture based techniques performed in non-ideal circumstances which prevail in resource poor settings. This study investigated the diagnostic utility World Health …

The Involvement Of Let-7 Mirna In The Regulation Of Retinal Progenitor Cells, Xiaohuan Xia

Theses & Dissertations

Emerging evidence has shown that miRNA-mediated gene regulation plays an important role in the development of the central nervous system (CNS). One of the developmental mechanisms may involve let-7 miRNAs and their up-stream regulator, Lin28a and Lin28b in regulating neural stem cells (NSCs). Recently, let-7 has been observed to influence the differentiation of NSCs along neuronal versus glial lineage. However, there is also a report suggesting that the neurogliogenic decision is independent of let-7. These paradoxical results might represent contextual roles of let-7 and lin28 during the CNS development. We have tested this premise in the mammalian retina, a reliable …

Investigation Of Behavioral And Cellular Changes In The Maternal Immune Activation Model Of Autism Spectrum Disorders, Shreya Roy

Theses & Dissertations

Maternal infection during pregnancy, which leads to maternal immune activation (MIA), is an environmental risk factor for autism spectrum disorders (ASD). MIA can be induced in mice and their offspring exhibit behaviors that model the core symptoms of ASD. One of the core behavioral symptoms in ASD patients is presence of increased repetitive behavior, which is modeled by an increase in marble burying in MIA mice. It has been shown that the deficits seen in MIA mice are associated with the dysregulation of cytokine levels in the developing brain, specifically an increase in pro-inflammatory cytokines. In this thesis, I tested …

Impact Of Estrogen Receptor Alpha On Sle1-Induced Loss Of Tolerance, Shayla D. Yoachim

Theses & Dissertations

The autoimmune disease lupus shows a significant female sex bias. This sex bias may be due to the ability of estrogens to promote loss of tolerance to chromatin, the initial loss of tolerance event in lupus. Previously, we demonstrated that the ability of estrogens to promote lupus in (NZBxNZW) F1 mice is dependent on signaling via estrogen receptor alpha (ERα). The Sle1 lupus susceptibility allele controls loss of tolerance to chromatin, and C57BL/6 (B6) mice carrying the Sle1 lose tolerance and develop anti-chromatin autoantibodies and spontaneously activated immune cells. Loss of tolerance occurs earlier and with a higher penetrance in …

Prevention Of Experimental Periodontitis In Rats With A Simvastatin Prodrug, Aaron Bradley

Theses & Dissertations

Introduction: Simvastatin (SIM) is a hypolipidemic drug that has been shown to have anti-inflammatory and bone anabolic properties. The aim of this study was to examine the effects of novel locally-applied SIM conjugated with methoxypolyethylene glycol to form micelles (SIM/SIM-mPEG) in an experimental periodontitis model in rats using micro-computed tomography (µ-CT) and histologic evaluation.

Methods: Experimental periodontitis was induced using silk ligatures around the maxillary right second molar (M2) in 40 Sprague-Dawley rats. Rats were divided into five groups using SIM/SIM-mPEG at 0.5, 1.0, or 1.5 mg SIM doses, mPEG-alone or no drug (ligature-alone) injected into the palatal M1-M2 gingiva …