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Understanding The Barrier And Facilitators Of Lifestyle Intervention Programs For Preventing Diabetes In High Risk Hispanic Adults, Lisa Adorno Dimaria

Open Access Dissertations

Since the Diabetes Prevention Program Research Group (2002) concluded that type 2 diabetes can be prevented or delayed in pre-diabetic individuals, translation of the study's intensive lifestyle intervention has been a research priority. Translational research conducted with the high risk Hispanic population is in its infancy. In general, the needs of this population have been neglected in the development and refinement of interventions to prevent T2D. This qualitative study explored intervention components and the perceived facilitators and barriers to making and sustaining dietary and physical activity behavior changes within the context of a lifestyle intervention program designed for a high …

A Numerical Study Of The Circulation, Ecology And Biogeochemistry On The Southern New England Shelf, Shuwen Zhang

Open Access Dissertations

This Ph.D. dissertation presents a process-oriented study with two primary objectives: 1) to obtain a physical understanding of the impacts of local and remote forcing, including basin-scale natural climate variability, on the interannual variability of along-shelf transport and water properties of the Middle Atlantic Bight (Chapter 1); 2) to explore the impacts of physical processes on the seasonal variation of phytoplankton biomass in Rhode Island coastal waters (Chapter 2). To achieve the two objectives, we apply a numerical modeling approach employed by the Regional Ocean Modeling System (ROMS) together with substantive analyses of both in-situ and remote observations.

Chapter 1 …

Regioselective Carbon-Nitrogen Bond Formation Via Oxidative Transition Metal Catalysis, Louis Marchetti

Open Access Dissertations

The synthesis of carbon-nitrogen (C-N) bonds is an area of research that has garnered significant interest due to the ubiquity of C-N bonds in pharmaceuticals and natural products. Various methods exist for forming C-N bonds, most recognizably Buchwald-Hartwig amination, but these reactions typically require functionalization of either the C-H or N-H bond resulting in the formation of harmful byproducts. As a result, the need to explore alternative means for synthesizing C-N bonds merits consideration. An alternative means for achieving C-N bond formation lies in the oxidative cross coupling of carbon-hydrogen (C-H) and nitrogen-hydrogen (N-H) bonds. While this approach eliminates the …

Bridging The Soft-Skill Gap From Education To Employment Through Internships, Kim Stack Washor

Open Access Dissertations

Employers express a growing concern that recent college graduates do not possess the necessary soft-skills to transition into entry level positions seamlessly. Educators are asked by employers and policy makers to provide instruction which would develop student skills in both the “hard” (academic and technical) and “soft” (personality traits and habits) skills required to be workplace-ready. The research study was designed to understand the degree to which internships enhance student soft-skill development, specifically in the areas of communication, teamwork, initiative, and, analytical thinking. Researcher-designed pre-post retrospective surveys were administered to students and one to corresponding supervisors to measure change in …

Development Of New Tools For Study Of Tumor Microenvironment, Michael D. Anderson

Open Access Dissertations

Solid tumors have a microenvironment that is inherently acidic and hypoxic. Hypoxia is caused by leaky blood vessels and large diffusion distances from cells to them. It is heterogeneous throughout the tumor and while all solid tumors are hypoxic to a degree, it is difficult to predict invasiveness based on it. However, acidity is a near ubiquitous characteristic of tumors with more aggressive tumors producing greater acidity. It is important to measure pH in diseased tissue with accuracy and precision, since acidity is associated with the development of various pathological states including tumors. In this work we focus on the …

Examining Health Professional Students’ Attitudes On Interprofessional Education, Suzanne Carr

Open Access Dissertations

There is a growing need for healthcare teams to effectively collaborate and communicate to improve patient outcomes. The need to improve patient care has been well established and cited by the Institute of Medicine’s (IOM) Committee on Quality Health Care in America landmark report, To Err Is Human: Building a Safer Health System (IOM, 1999; Kohn, Corrigan, & Donaldson, 2000). Health educational programs are known to be a gateway to changing behaviors of health professionals to impact practice and direct patient care. Interprofessional Education (IPE) has been identified as a viable mechanism to increase the collaboration and communication of health …

Adp-Ribosylation Factor 6 (Arf6) Regulates Integrin Αiibβ3 Trafficking, Platelet Spreading, And Clot Retraction, Yunjie Huang

Theses and Dissertations--Molecular and Cellular Biochemistry

Endocytic trafficking of platelet surface receptors plays a role in the accumulation of granule cargo (i.e. fibrinogen and VEGF) and thus could contribute to hemostasis, angiogenesis, or inflammation. However, the mechanisms of platelet endocytosis are poorly understood. The small GTP-binding protein, ADP-ribosylation factor 6 (Arf6), regulates integrin trafficking in nucleated cells; therefore, we posited that Arf6 functions similarly in platelets. To address this, we generated platelet-specific, Arf6 knockout mice. Arf6^-/- platelets had a storage defect for fibrinogen but not other cargo, implying Arf6’s role in integrin αIIbβ3 trafficking. Additionally, platelets from Arf6^-/- mice injected with biotinylated-fibrinogen, showed …

Mechanisms Of Therapeutic Resistance In Castration Resistant Prostate Cancer, Sarah Katherine Martin

Theses and Dissertations--Molecular and Cellular Biochemistry

Taxane based chemotherapy is an effective treatment for castration-resistant prostate cancer (CRPC) via stabilization of microtubules. Progression to castration-resistant prostate cancer is characterized by increased androgen receptor (AR), elevated intra-prostatic androgens and activated AR signaling despite castrate levels of androgens.

Previous studies identified that the inhibitory effect of microtubule targeting chemotherapy on AR activity was conferred by interfering with AR intracellular trafficking. The N-terminal domain (NTD) of AR was identified as a tubulin interacting domain that can be effectively targeted by the novel small molecular inhibitor, EPI. Taken together, this evidence provided the rationale that targeting AR nuclear translocation and …

The Role Of Phlpp In Pancreatic Cancer, Alena J. Smith

Theses and Dissertations--Molecular and Cellular Biochemistry

Medicine has come a long way in recent years with reliable treatments for many cancers. Pancreatic ductal adenocarcinoma (PDAC) has very few treatment options available. PDAC has a dismal 5 year survival rate of 4% and a median survival span of 6 months from point of diagnosis; with a high rate of chemotherapy and radiation resistance. A better understanding of the molecular events leading to cancer progression is needed in order to improve the treatment and prognosis of PDAC patients. We begin to elucidate the functional importance of PHLPP on suppressing progression and metastasis of PDAC. PHLPP belongs to a …

Understanding The Stressors Of Low Socioeconomic Rural Parents Of Autistic Children, Wendi Marissa Dunham

Walden Dissertations and Doctoral Studies

Parents of children with autism spectrum disorder (ASD) often exhibit high parental stress which may be caused by parent-child behaviors, child behaviors, and parent distress. Awareness and understanding of ASD is critical for parents and educators to connect students with support services and resources. The purpose of this bounded, descriptive case study was to identify perceived parental stressors and to determine the primary stressors of parents with low socioeconomic status who are raising a child with ASD in a rural area. The foundation of family systems theory framed this study. Ten parents raising a child with ASD were purposefully selected …

The Center For Total Health: Healthcare Reform In Cook County, Illinois, James Leon Miles

Walden Dissertations and Doctoral Studies

The Patient Protection and Affordable Care Act (PPACA) of 2010 requires hospital systems in the United States to shift the culture of patient care from a focus on sick-care to a focus on prevention and wellness care. Little is known about how hospital systems will make this culture shift while retaining quality patient care. The purpose of this case study of a pioneering hospital-based PPACA-compliant initiative was to answer the research question of how Wallace's revitalization movement theory (RMT) "a rapid culture change model"could serve as a transferable evaluation framework for PPACA prevention and wellness care compliance in hospital-based programs. …

The Deterrent Effect Of Disciplinary Segregation On Prison Inmate Misconduct, Joseph William Lucas

Walden Dissertations and Doctoral Studies

Although a widely used practice, it was previously unknown whether disciplinary segregation is actually effective at modifying prison behavior. This quantitative, retrospective observational study tested deterrence theory and explored the effectiveness of disciplinary segregation in deterring subsequent prison inmate misconduct among those subjected to it (N = 228). It compared a cohort of male inmates incarcerated by the Oregon Department of Corrections who had spent time in disciplinary segregation in 2011 and/or 2012 with a comparison cohort who had not spent any time in disciplinary segregation. Three models were tested, each with the outcome variable operationalized in a different way: …

A Structure/Function Analysis Of Nhsl1b In Facial Branchiomotor Neurons, John Ojumu

Theses and Dissertations

The goal of this study was to identify critical regions of a novel gene, Nance-Horan syndrome-like 1b (nhsl1b). It was previously discovered that C-terminal truncation of the Nhsl1b protein in nhsl1b mutants resulted in a loss of migration in the facial motor neurons of the hindbrain (Walsh et al. 2011). As nhsl1b expresses many isoforms, multiple targets were investigated in order to determine which transcript bears the largest impact on the motor neurons. Using confocal microscopy to observe immunostained embryos, we examined a mutation in an nhsl1b transcript that encodes a WHD, a domain that is known to …

The Effect Of Statins On Il-33 Mediated Mast Cell Function, Marcela Taruselli

Theses and Dissertations

This study demonstrates original findings of statin effects on IL-33 stimulated mast cells. Statins are a class of drugs used to lower cholesterol production by targeting HMG CoA reductase. These commonly prescribed drugs have been shown to be immunomodulatory. In this study, we have found that pretreatment with statins has a variety of effects on IL-33 stimulated mast cells. Atorvastatin suppresses TNF and IL-6 production, while fluvastatin significantly enhances release of these proinflammatory cytokines in BMMCs. Although they have differing effects on cytokine production, both statins lowered ST2 expression on the cell surface, decreased cell viability, and enhanced expression of …

The Export Of An Algal Toxin Into Terrestrial Predators Via Emerging Aquatic Insects, Nicholas J. Moy

Theses and Dissertations

Algal blooms are directly related to human-caused nutrient enrichment of water bodies. The cyanobacteria Microcystis aeruginosa produces microcystin (MC), a toxin that has been linked with mortalities and illness of many organisms. We show that MC is not constrained by the aquatic-terrestrial ecotone. MC was detected in a primary consumer and emerging aquatic invertebrate (Hexagenia Mayfly), a terrestrial insect and predator of emerging aquatic invertebrates (Tetragnathidae Spider), and a vertebrate consumer (Prothonotary Warbler). Mayfly and spider MC levels varied across the blooming period. MC levels in prothonotary warbler livers varied by age class; nestlings having the highest levels. MC levels …

Primary Cilia Mechanotransduction And Microtubule Stability In Mechanically Stretched Lung Adenocarcinoma Cells, Monika Rassi Radhika

Theses and Dissertations

The objective of this study is to investigate the role of microtubule based organelle, the primary cilia in lung adenocarcinoma by i) Quantifying the presence of primary cilia in several Non Small Cell Lung Cancer (NSCLC) cell lines in response to mechanical stimuli, ii) Attempting to determine the role of primary cilia in cell migration, iii) Investigating the effects of Paclitaxel(Taxol) resistance in lung cancer cells, iv) Analyzing the response of lung cancer cells to Smoothened Inhibitors and v) Determining the effects of Transforming Growth Factor Beta-1(TGF-β1) induced Epithelial to Mesenchymal Transition(EMT) in lung cancer cells. To ascertain the effects …

Ex Vivo Dna Cloning, Adam B. Fisher

Theses and Dissertations

Genetic engineering of microbes has developed rapidly along with our ability to synthesize DNA de novo. Yet, even with decreasing DNA synthesis costs there remains a need for inexpensive, rapid and reliable methods for assembling synthetic DNA into larger constructs or combinatorial libraries. While technological advances have resulted in powerful techniques for in vitro and in vivo assembly of DNA, each suffers inherent disadvantages. Here, an ex vivo DNA cloning suite using crude cellular lysates derived from E. coli is demonstrated to amplify and assemble DNA containing small sequence homologies. Further, the advantages of an ex vivo approach are …

Comparing Welch's Anova, A Kruskal-Wallis Test And Traditional Anova In Case Of Heterogeneity Of Variance, Hangcheng Liu

Theses and Dissertations

Analysis of variance (ANOVA) is a robust test against the normality assumption, but it may be inappropriate when the assumption of homogeneity of variance has been violated. Welch ANOVA and the Kruskal-Wallis test (a non-parametric method) can be applicable for this case. In this study we compare the three methods in empirical type I error rate and power, when heterogeneity of variance occurs and find out which method is the most suitable with which cases including balanced/unbalanced, small/large sample size, and/or with normal/non-normal distributions.

Functional Characterization Of Rai1 In Zebrafish, Joshua S. Beach

Theses and Dissertations

Smith-Magenis Syndrome (SMS; OMIM #182290) is a multiple congenital abnormality and intellectual disability (ID) disorder caused by either an interstitial deletion of the 17p11.2 region containing the retinoic acid induced-1 (RAI1) gene or a mutation of the RAI1 gene. Individuals diagnosed with SMS typically present characteristics such as ID, self-injurious behavior, sleep disturbance, ocular and otolaryngological abnormalities, craniofacial and skeletal abnormalities, neurological and behavioral abnormalities, as well as other systemic defects and manifestations. Previous work by Vyas in 2009 showed temporal expression of rai1 in zebrafish embryos as early as 9 hpf. We hypothesize that there is maternal …

Skills Women Bring To The Position Of Chief Of Police, Jo-Ann Helen Savoie

Walden Dissertations and Doctoral Studies

Organizational leaders are unaware of the gender-specific leadership skillsets women possess to increase organizational effectiveness and how to address potential barriers for assuring these skillsets are recognized as effective. Of the estimated 69,000 police officers serving in Canada, approximately 14,000 are women. Of those 14,000, only 10% hold a senior rank, and less than 3% hold the position of Chief of Police. Technology speed, globalized crime, and shrinking budgets have created a need for a new style of leader in policing, and increasing the representation of women may address this need. This multiple case study used the concept of doing …

Effects Of Nicotinic Acetylcholine Receptor Agonists In Assays Of Pain-Stimulated And Pain-Depressed Behavior In Rats, Kelen Freitas

Theses and Dissertations

Though a host of analgesics have been developed to alleviate pain, especially acute pain, significant side effects and a lack of long-term efficacy have encouraged research attempts to pursue novel targets that may be associated with fewer side effects or a more sustained efficacy. Among these new targets are members of the nicotinic family of acetylcholine receptors (nAChRs). The non-selective nAChR agonists nicotine and epibatidine have been shown to function as potent antinociceptive drugs in many acute and chronic preclinical pain models, while nicotine has produced analgesic effects in humans. However, these non-selective nAChRs agonists also produce various side effects, …

Rheb Dynamics On Lysosomal Membranes Determines Mtorc1 Activity After Loss Of P53 Or Activation Of Ampk, Catherine M. Bell

Theses and Dissertations

The tumor suppressor TP53 is the most frequently altered gene in human cancers. The growth-promoting complex, mTORC1 plays a part of the oncogenic profile caused by dysfunctional p53. mTORC1 sits downstream of AMPK and other crucial tumor suppressors/oncogenes, PTEN, LKB1, and Akt. The antifolate pemetrexed was found by this laboratory to activate AMPK via the inhibition of the enzyme AICART in de novo purine synthesis. This work presents a mechanism of mTORC1 activation with p53 loss, as well as of mTORC1 inhibition by pemetrexed-induced AMPK. We have found that mTORC1 activity was substantially upregulated by the loss …

Synthetic Cannabinoids Versus Delta-9-Tetrahydrocannabinol: Abuse-Related Consequences Of Enhanced Efficacy At The Cannabinoid 1 Receptor, Travis Grim

Theses and Dissertations

In the past ten years, synthetic cannabinoids (SC) have emerged as drugs of abuse. Unlike D⁹-tetrahydrocannabinol (THC), many SCs are associated with serious health complications and death. One way in which THC and SCs differ lies with their enhanced potency and efficacy at the CB₁ receptor. No current methods exist to measure efficacy at the CB₁ receptor in vivo, and the abuse-related properties of SC cannabinoids are not well explored. Here, we utilized CB₁ wild type (WT), heterozygous (HET), and knockout (KO) mice. By employing CB₁ ligands which differ in efficacy we have …

In-Silico Models For Capturing The Static And Dynamic Characteristics Of Robustness Within Complex Networks, Bhanu K. Kamapantula

Theses and Dissertations

Understanding the role of structural patterns within complex networks is essential to establish the governing principles of such networks. Social networks, biological networks, technological networks etc. can be considered as complex networks where information processing and transport plays a central role. Complexity in these net works can be due to abstraction, scale, functionality and structure. Depending on the abstraction each of these can be categorized further. Gene regulatory networks are one such category of biological networks. Gene regulatory networks (GRNs) are assumed to be robust under internal and external perturbations. Network motifs such as feed-forward loop motif and bifan motif …

The Effects Of Family Functioning And Tangible Support On Treatment Outcomes In An Opioid Addicted Population, Nathasha N. Cole

Theses and Dissertations

The effects of family functioning and tangible support from family members are examined in an opiate addicted population. The study specifically assessed drug use, self-efficacy, and quality of life as treatment outcomes of interest. There have been mixed findings in the literature in regards to how families influence rehabilitation from substance use. Specially, previous research has shown that families can further patients’ recovery, while other findings have shown that families can impede patients’ recovery from substance use. The aim of this study was to analyze potentially contributing factors related to the family system, to gain a stronger understanding of how …

Functional Local Renin-Angiotensin System In Human And Rat Periodontal Tissue, Carlos Ferreira Santos, Ana Carolina Morandini, Thiago José Dionísio, Flávio A. Faria, Marta C. Lima, Caio M. Figueiredo, Bella L. Colombini-Ishikiriama, Carla Renata Sipert, Rubens P. Maciel, Ana P. Akashi, Gabriela P. Souza, Gustavo P. Garlet, Camila O. Rodini, Sandra L. Amaral, Christiane Becari, Maria C. Salgado, Eduardo B. Oliveira, Isaac Matus, Daniela N. Didier, Andrew S. Greene

All Dugoni School of Dentistry Faculty Articles

The initiation or progression of periodontitis might involve a local renin-angiotensin system (RAS) in periodontal tissue. The aim of this study was to further characterize the local RAS in human and rat periodontal tissues between healthy and periodontally-affected tissue. Components of the RAS were investigated using in vitro, ex vivo and in vivo experiments involving both human and Wistar rat periodontium. Although not upregulated when challenged with P. gingivalis-lipopolysaccharide, human gingival and periodontal ligament fibroblasts expressed RAS components. Likewise, healthy and inflamed human gingiva expressed RAS components, some of which were shown to be functional, yet no differences in expression …

The Increasing Weight Of Regulation: Countries Combat The Global Obesity Epidemic, Emily Whelan Parento, Allyn L. Taylor, Laura A. Schmidt

McGeorge School of Law Scholarly Articles

Obesity is a global epidemic, exacting an enormous human and economic toll. In the absence of a comprehensive global governance strategy, states have increasingly employed a wide array of legal strategies targeting the drivers of obesity. This Article identifies recent global trends in obesity-related legislation and makes the normative case for an updated global governance strategy. National governments have responded to the epidemic both by strengthening traditional interventions and by developing novel legislative strategies. This response consists of nine important trends: (1) strengthened and tailored tax measures; (2) broadened use of counter-advertising and health campaigns; (3) expanded food labeling; (4) …

Developing Proteomic And Cytokine Biomarkers For Vulvodynia, Ashvin Iyer

Browse all Theses and Dissertations

Vulvodynia is a chronic, heterogeneous, and multifactorial disease. This condition may affect up to 18 percent of the female population including Caucasians, African Americans, Africans and Hispanics particularly those sexually active at child bearing age. The etiology of this condition is complex and multifactorial and it is frequently accompanied by physical disabilities, psychological distress and sexual dysfunction. Clinically, vulvodynia can be generalized or localized and pain can be provoked or unprovoked. Patients may also describe vulvar paresthesias or dysesthesias that may last hours. The International Society for the Study of Vulvar Disease (ISSVD) recognizes vulvar pain related to a specific …

Manipulation Of Gold Nanorod Physicochemical Properties To Enhance Biocompatibility, Uptake And Intracellular Preservation Of Optical Properties For Bio-Imaging And Plasmonic Photo-Therapeutic Applications, Anthony B. Polito

Browse all Theses and Dissertations

Gold nanorods (GNRs) due to their tunable optical properties within the near infrared (NIR) region have been identified as strong candidates for new nano-based biomedical applications. Unfortunately, many have reported GNR cytotoxicity and it is still unclear how GNR aspect ratio (AR), surface charge and surface chemistry contribute to cellular association and cytotoxicity. GNR surface chemistry modifications have been reported to reduce cytotoxicity, however they can result in poor cellular uptake and loss of NIR optical properties preventing efficiency with bio-imaging and photo-thermal applications. The aim of this study was to determine if manipulation of GNR physicochemical properties could enhance …

Na+/K+ Pump And Cl--Coupled Na+ And K+ Co-Transporters In Mouse Embryonic Fibroblasts Lacking The Tuberous Sclerosis Complex Tsc1 And Tsc2 Genes., Jasser Ali S. Alzhrani

Browse all Theses and Dissertations

The tuberous sclerosis complex 1 and 2 (TSC1 and TSC2) genes and their products hamartin and tuberin, respectively, are involved in several regulatory cellular mechanisms, including the mTOR pathway that is important for proliferation, cell migration, and protein synthesis. In addition, it has been suggested that this pathway plays a role in regulating Na+-K+-2Cl- and K+-Cl- cotransporters (NKCC and KCC, respectively) [41, 42, 43]. Therefore, the TSC1 and TSC2 genes are expected to play a significant role in regulating cell volume, and K+ and Cl- homeostasis. This study tests the hypothesis that the TSC1 and TSC2 genes, which control cell …