Digital Commons Network^™

The Impact Of Dreissenid Mussels On Growth Of The Fragile Papershell (Leptodea Fragilis), The Most Abundant Unionid Species In Lake Erie, Robert A. Krebs, Elizabeth M. Barkett, Matthew T. Begley

Biological, Geological, and Environmental Faculty Publications

The arrival of zebra mussels (Dreissena polymorpha (Pallas, 1771)) and subsequently quagga mussels (Dreissena bugensis Andrusov, 1897) (Dreissenidae) in the Great Lakes in the 1980s induced many changes, most notably the devastation of native freshwater mussel species. Recently, empty shells of the fragile papershell (Leptodea fragilis (Rafinesque, 1820)) have become common, particularly in the western basin of Lake Erie, suggesting that this fast-growing species may be increasing in numbers in the lake. To examine continued competition with dreissenids, shell age and length of L. fragilis were used to contrast lifespan and growth rate, estimated as the slope …

Dna Double-Strand Breaks And Telomeres Play Important Roles In Trypanosoma Brucei Antigenic Variation, Bibo Li

Biological, Geological, and Environmental Faculty Publications

Human-infecting microbial pathogens all face a serious problem of elimination by the host immune response. Antigenic variation is an effective immune evasion mechanism where the pathogen regularly switches its major surface antigen. In many cases, the major surface antigen is encoded by genes from the same gene family, and its expression is strictly monoallelic. Among pathogens that undergo antigenic variation, Trypanosoma brucei (a kinetoplastid), which causes human African trypanosomiasis, Plasmodium falciparum (an apicomplexan), which causes malaria, Pneumocystis jirovecii (a fungus), which causes pneumonia, and Borrelia burgdorferi (a bacterium), which causes Lyme disease, also express their major surface antigens from loci …

Christian Higher Education At The Start Of The 21st Century: A Review Essay, David Johnstone

Publications from Student Life & Spiritual Life

Every few years, evangelical scholars publish volumes addressing the nature of how their faith interacts with their academic discipline. While it would seem to be a task that does not need to be repeated, each new project is distinctive to the context in which it is written. Our world is different from 10 years ago, contributing to changes regarding how the conversation looks and develops. Some material is timeless, while other information changes. Currently, underlying the faith and discipline conversation is the utilitarian response that asks, “What makes a Christian college so special?” The implication is that one could gain …

Studying The Modular Mechanisms Of Permeabilizing And Translocating Chimeric Peptide Combinations, Maria A. Labouyer

Non-Honors Thesis Collection

No abstract provided.

Optimization Of Co-Immunoprecipitation Assays To Determine The Molecular Mechanism Modulating Direct Interactions Between Potassium Channel Α-Subunit Proteins Herg And Kvlqt1, Estelle Kim, Louise E.O. Darling

Non-Honors Thesis Collection

No abstract provided.

Tennessee Oversight Agreement Status Report To The Public Fiscal Year 2015, Tennessee. Department Of Environment And Conservation. Division Of Department Of Energy Oversight

Annual Status Reports

This report was produced by the Tennessee Department of Environment and Conservation, Division of Remediation, DOE Oversight Office.

Esat-6 Targeting To Dec205+ Antigen Presenting Cells Induces Specific-T Cell Responses Against Esat-6 And Reduces Pulmonary Infection With Virulent Mycobacterium Tuberculosis, Aarón Silva-Sánchez, Selene Meza-Pérez, Adriana Flores-Langarica

Publications

Airways infection with Mycobacterium tuberculosis (Mtb) is contained mostly by T cell responses, however, Mtb has developed evasion mechanisms which affect antigen presenting cell (APC) maturation/recruitment delaying the onset of Ag-specific T cell responses. Hypothetically, bypassing the natural infection routes by delivering antigens directly to APCs may overcome the pathogen's naturally evolved evasion mechanisms, thus facilitating the induction of protective immune responses. We generated a murine monoclonal fusion antibody (α-DEC-ESAT) to deliver Early Secretory Antigen Target (ESAT)-6 directly to DEC205⁺ APCs and to assess its in vivo effects on protection associated responses (IFN-γ production, in vivo CTL killing, and …

17th Annual Student Academic Conference, Minnesota State University Moorhead

Student Academic Conference Abstract Books

Minnesota State University Moorhead Student Academic Conference abstract book.

The Failure Of Environmental International Law During Times Of War, Blake Lara

University of Baltimore Journal of Land and Development

Throughout history, war and armed conflict have maintained a continuous presence around the world. Though the reasons for war change, various nations emerge and subside, and populations alter, one of the constant elements of war is its degrading effect on the environment. In addition to indirect effects on the environment that ultimately result from war, nations have used the environment as both a weapon and target of war. For example, during the Peloponnesian War, the Spartans salted Athenian lands to make them infertile. In the Franco-Dutch War from 1672 to 1678, dikes and damns were destroyed in order to create …

Mirabile Dictu: The Bryn Mawr College Library Newsletter 18 (2015), Bryn Mawr College Library

Mirabile Dictu: Newsletter of the Bryn Mawr College Libraries

No abstract provided.

University Of South Alabama College Of Medicine Annual Report For 2015-2016, College Of Medicine

COM Annual Reports

This Annual Report of the College of Medicine catalogues recent accomplishments of our faculty, students, residents, fellows and staff in teaching, research, patient care, scholarly and community service activities during the 2015-16 academic year.

Wind, Ice, And Bugs: Impacts In The Gordon Natural Area, Gerard Hertel, Kendra Mcmillin

Historical Tree Fall Study Documents

No abstract provided.

Effects Of Excess Nitrogen Deposition On Rubus Spp. (Raspberry) Within A Central Appalachian Hardwood Forest, Jake H. Billmyer

Theses, Dissertations and Capstones

This thesis contributes to on-going research at Marshall University on effects of excess nitrogen (N) deposition on hardwood forests. Excess N can decrease plant biodiversity and enhance loss of nutrients (e.g., Ca⁺⁺). Preliminary results have suggested that excess N has increased cover of Rubus (blackberry) in the herbaceous layer. The purpose of this study was to quantify the response of Rubus to the N treatment, relating Rubus cover to species richness and using foliar analysis to examine effects on nutrient availability. It is expected that increased Rubus will decrease biodiversity by eliminating N-efficient species, and that added N …

Cancer Stem Cells In The Screening Of Anticancer Drugs For Central Nervous System Tumors, Sarah Elizabeth Daron-Mathis

Theses, Dissertations and Capstones

There is a growing need (in the medical field) to design personalized therapy for cancer patients. Decades of cancer research have found no silver bullet that can cure all or even most patients. This study evaluated four patients affected by central nervous system (CNS) tumors (Ependymoma and Glioblastoma), and found that tumors with the same histology had unique responses to treatment. Each sample presented different levels of heterogeneity in expressed biomarkers and responded to drugs at varying levels.

Oncologists conventionally treat cancer patients with drugs tested in large clinical trials. However, often patients do not experience positive outcomes following treatments …

Population Level Responses To Direct Application Liming In Gyrinophilus Porphyriticus, Shelby Renea Timm

Theses, Dissertations and Capstones

Direct application liming (DAL) has been used to neutralize acidified streams to restore aquatic biota. This mitigation technique has been used globally for decades, yet little data exist on its effects on amphibian populations. My study investigated the effects of liming on amphibians by measuring variability in life histories of larval Gyrinophilus porphyriticus. I collected larvae from six streams in the Monongahela National Forest, West Virginia. I examined the effects of DAL on age structure, and I failed to detect a treatment effect. I used ANCOVAs to examine differences in body condition, body size, and gape size. I observed that …

Investigation Of Ultrasound Targeted Microbubbles As A Therapeutic Gene Delivery System For Prostate Cancer, Rounak Paramjeet Nande

Theses, Dissertations and Capstones

A major challenge for effective gene therapy is systemic delivery of viruses carrying therapeutic genes into affected tissue. The immunogenic nature of human adenoviruses (Ads) limits their use for intratumoral (IT) injection in gene therapy. Ads transfection is further hampered by the fluctuating presence of Coxsackie and Adenovirus Receptor (CAR) and integrins on the cells’ surface. To circumvent these limitations we developed a novel approach wherein Ads are encapsulated inside the shell of lyophilized, lipid-encapsulated, perfluorocarbon microbubbles (MBs)/ultrasound (US) contrast agents, which act as delivery vehicles for a sitespecific gene transfer system.

We performed infection studies with Ad.GFP (Green Fluorescent …

Exploring The Disassociation Between Corporations, Humans, And Nature, Steven Lee Smith

Theses, Dissertations and Capstones

The aim of this thesis is to investigate and analyze the disassociation between corporations, humans, and nature using the Eco-Marxist and Posthumanism literary theories.

In the first chapter, coal politics of the Appalachian region of the United States are explored using the Eco-Marxist literary theory. This theory allows one to examine how corporations undermine communities and nature for financial gain. Because current research was lacking in regards to local and national coal politics, the author decided to examine an area that was familiar with corporate hegemony.

In the second chapter, the issue of bioethics in factory farms is investigated using …

Evaluating Potential Plant Hormone Cross Talk Between Auxin And Ethylene In Arabidopsis, Mia Lynne Brown

Theses, Dissertations and Capstones

Auxin is the primary hormone responsible for plant growth and development. Regulation of auxin-response genes occurs through transcriptional activators (auxin response factors called ARFs) which bind to auxin response elements (AuxREs). Some auxin-responsive genes encode aminocyclopropane-1-carboxylic acid synthase (ACS) enzymes which regulate the production of the plant hormone ethylene. The major research objective was to evaluate transcriptional cross talk between auxin and ethylene. Both AuxREs and ethylene response elements were found in several ACS and ARF genes, suggesting cross talk between the two hormones at the transcriptional level. Analysis of transgenic Arabidopsis thaliana plants deficient in ARF7 and containing a …

An Investigation Of The Effect Of Mn2+ On The Fth1 Ire-Irp Complex, Eric Thomas Mendenhall

Theses, Dissertations and Capstones

Iron is a widely distributed metal found in almost every physiological system in nature, thus being an essential part of life. Iron homeostasis in the human body must be tightly regulated as iron is both beneficial and harmful to different cellular processes. Ferritin is a protein that has a prominent role in maintaining iron homeostasis via iron sequestration. Additionally, this protein itself is regulated by the Iron Responsive Element (IRE) and its interaction with an apoaconitase protein or the Iron Regulatory Protein (IRP). The binding of the IRP to this IRE, located on the 5’ untranslated stem loop region of …

Correlates Of Exhibition-Like Experiences And The Development Of Exhibitionism In Females, Sarah Fouch

Theses, Dissertations and Capstones

The purpose of this study was to examine possible events or risk factors that occur during the critical period of learning, before age 18, that influence an individual to engage in exhibitionism or have urges to expose themselves. Anonymous data from 2,201 female participants were obtained using a computer-assisted self-administered questionnaire. Early experiences were examined to determine risk factors for ever having exposed themselves in a public place or ever having had urges to expose themselves in public places using logistic regression analysis. Many of the events that were identified as significant predictors for exhibitionism, such as seeing the mother …

Sampling Considerations For Amphibian Surveys: Evaluating Risks Of Committing Type I And Type Ii Errors, Kelli Marie Herrick

Theses, Dissertations and Capstones

Global amphibian declines pose a major threat to the world’s biodiversity. We examined the observation bias associated with volunteer based anuran surveys, such as the North American Amphibian Monitoring Protocol (NAAMP). We followed NAAMP protocol to examine if variation in the persons (1-3) in an observer unit affected observer error. We hypothesized that observation units with multiple observers have less observer bias and would better report anuran assemblages compared to single observers. Larger observer units had fewer incidences of false positive observations. Additionally, we attempted to determine which sampling method for the eastern hellbender (Cryptobranchus a. alleganiensis) had the highest …

Epigenetic Role Of Ptip In Mouse Spermatogenesis, Chengjing Liu

Theses, Dissertations and Capstones

In mammals, spermatogenesis is a biological process inside the testis to produce spermatozoa from spermatogonia. This process is governed by both genetic and epigenetic mechanisms and thus is a powerful system for epigenetic research. Methylation of histone 3 lysine 4 (H3K4) is an epigenetic mark, which has been found to be dynamically modulated in mouse male germ cells during spermatogenesis. Pax2 Transactivation domain Interaction Protein (PTIP) has been recently identified as part of a H3K4 methyltransferase complex. In this study, I hypothesize that PTIP is an essential epigenetic regulator in mouse spermatogenesis. To test this hypothesis, I first established a …

Nuclear Family Dynamics: Predictors Of Childhood Crushes And Adult Sexual Orientation, Natalie M. Campbell

Theses, Dissertations and Capstones

This study investigated the effects of nuclear family dynamics on childhood crushes and adult sexual orientation. Retrospective data was gathered via an anonymous survey administered using a computer-assisted self-interview program. Data was collected from 1,242 non-transsexual males (age range 18-86) and 2,201 non-transsexual females (age range 18-78). Management of parental disagreements, maternal attitude about sex, parental composition of the nuclear family, maternal affection, and parental demonstration of affection predicted the sex of childhood crushes in males and/or females. Management of parental disagreement, parental attitude about sex, nudity practices within the home, parental demonstration of affection, and parental composition of the …

Functional Studies Of Ptchd3 During Spermatogensis, Shaimar R. González Morales

Theses, Dissertations and Capstones

Paracrine factor Desert hedgehog (Dhh) is essential for mouse spermatogenesis. However, the specific receptor of Dhh during spermatogenesis is unknown. This study aims to test the hypothesis that Ptchd3, a male germ cell-specific gene acts as a receptor for Dhh in spermatogenesis. In this study, a transgenic mouse model with Ptchd3 gene deletion was first successfully established. Then, in vivo fertility assay and in vitro analysis were performed on Ptchd3 null mutant male mice. The data obtained from the in vivo fertility experiments indicates that there is no statistical significance in offspring litter number (p-value 0.7973) and litter size (p-value …

Development Of Culturing Methods For Native Mayfly Taxa For Use In Laboratory Toxicity Testing, Kevin Nicholas Rowsey

Theses, Dissertations and Capstones

Salinity is increasing in freshwaters throughout the world due to anthropogenic impacts with the implication of the increases to biological communities only now being realized. Declining mayfly populations in Appalachian streams have generated increased interest in elevated dissolved solids in that region and their effect on benthic macroinvertebrates, particularly the sensitive Ephemeroptera taxa. Field and laboratory evaluations have indicated high sensitivity of mayflies to elevated dissolved solids. The research described herein is part of an ongoing effort to provide opportunity for toxicity testing with native mayflies in the laboratory. A successful endeavor would establish mayfly sensitivity to total dissolved solids …

Tell Me A Story: The Relationship Between Identity And Life Stories Following Brain Injury, Sara Henson

Theses, Dissertations and Capstones

Following brain injury, people can demonstrate cognitive-linguistic deficits as well as a shift in their identities. Clinicians use assessment and treatment methods that focus on cognitive-linguistic deficits with this population; however, they rarely (if ever) consider identity as a viable and necessary target for intervention efforts. For individuals with brain injury, life narratives can be used to assess and treat the cognitive-linguistic skills and discourse at a macro-systemic level, build relationships through the use of a client-centered approach, develop a strong sense of self-identity that aids in goal setting, establish life purpose that motivates greater participation and effort in rehabilitation, …

Glycation Of Lysozyme, Wisam Talib Joudah

Theses, Dissertations and Capstones

For this research project, I propose a method for the analysis of glycated lysozyme using Matrix Associated Laser Desorption / Ionization-Time of Flight Mass spectrometry (MALDI-TOF MS). Glycation or "Maillard reaction" is a non-enzymatic reaction that occurs between amino acid residues, usually primary and secondary amines, of proteins and the carbonyl group of reducing sugars to yield Schiff bases. The glycation mechanism occurs between the carbonyl group of the reducing sugars and a primary amine by nucleophilic addition, resulting in the formation of a Schiff base. The Schiff base can then rearrange into the more stable Amadori product which is …

Effects Of Roads And Trails On Peaks Of Otter Salamander (Plethodon Hubrichti) And Eastern Red-Backed Salamander (Plethodon Cinereus) Movement Behavior, Cory Benjamin Goff

Theses, Dissertations and Capstones

Habitat fragmentation negatively affects movement and dispersal of wildlife by altering useable space and creating barriers. The objective of this study was to assess impacts of fragmentation by roads and trails on the movement behavior of two terrestrial, woodland salamander species. I evaluated whether proximity to roads and trails affected the movement behavior of two species: the Peaks of Otter Salamander (Plethodon hubrichti), a species of special concern, and the Eastern Red-backed Salamander (Plethodon cinereus). I examined movement behavior in three treatments: paved roads, gravel roads and hiking trails. I compared treatments to controls located at least 200m from treatment …

Monitoring The Land Cover Change In The Historic Range Of Cambarus Veteranus In West Virginia Using A 1973-2013 Landsat Time Series, Emma Martha Arneson

Theses, Dissertations and Capstones

The crayfish Cambarus veteranus is near extinction in its historic range of the Upper Guyandotte River watershed. The biggest threats to C. veteranus are mining and road construction. Mining has been a continuous activity in the southern coalfields where the crayfish has historically been found, yet little is known about how much land cover change the practice has done to the region. Crayfish act as important organisms within aquatic ecosystems and without them, those systems are often degraded. Quantifying the change in land cover is important to understanding threats to C. veteranus for future protection of the crayfish and its …

Investigation Into The Biological Importance And Function Of Proinsulin C-Peptide, Christina L. Newsome

Theses, Dissertations and Capstones

The C-peptide of insulin was thought to be biologically inactive, but recent studies have shown that the C-peptide causes multiple molecular and physiological effects. Evidence has shown that C-peptide binds to a cell surface receptor, probably a G-protein coupled receptor, and that the COOH-terminal pentapeptide is essential for binding and constitutes an active site. For a further understanding of the detailed nature of the physiological effects of C-peptide, the receptor structure needs to be determined. We designed an affinity column using C-peptide to try and gain a better understanding of the biological effects by examining what proteins the affinity column …