Digital Commons Network^™

Computational Investigations Of Potential Energy Function Development For Metal-Organic Framework Simulations, Metal Carbenes, And Chemical Warfare Agents, Christian R. Cioce

USF Tampa Graduate Theses and Dissertations

Metal-Organic Frameworks (MOFs) are three-dimensional porous nanomaterials with a variety of applications, including catalysis, gas storage and separation, and sustainable energy. Their potential as air filtration systems is of interest for designer carbon capture materials. The chemical constituents (i.e. organic ligands) can be functionalized to create rationally designed CO2 sequestration platforms, for example. Hardware and software alike at the bleeding edge of supercomputing are utilized for designing first principles-based molecular models for the simulation of gas sorption in these frameworks. The classical potentials developed herein are named PHAST -- Potentials with High Accuracy, Speed, and Transferability, and thus are designed …

High-Throughput Screening Of Age-Related Changes In Caenorhabditis Elegans, Neil Copes

USF Tampa Graduate Theses and Dissertations

This project was developed to identify novel methods for high-throughput culturing and screening of C. elegans to investigate age-related metabolic changes and to survey the proteomic and metabolomic factors associated with age-related changes. To accomplish these goals we developed a novel way to grow C. elegans in liquid culture in 96-well microplates for several weeks without suffering significant fluid loss due to evaporation and without needing to shake or unseal the plates for aeration. We also developed methods for assaying the total volume of live C. elegans in microplate cultures using a fluorescence microplate reader and for performing RNAi experiments …

Strategies For Preventing Age And Neurodegenerative Disease-Associated Mitochondrial Dysfunction, Vedad Delic

USF Tampa Graduate Theses and Dissertations

Mitochondrial dysfunction plays a pivotal role in the development of aging phenotypes and aging-associated neurodegenerative disorders, such as Alzheimer’s disease (AD), Parkinson’s disease (PD) and Amyotrophic lateral sclerosis (ALS). Strategies that restore mitochondrial dysfunction may rescue the deficits of central metabolism in these disorders and improve cell survival. For example, we found that modulating the mTOR signaling pathway in a tissue culture model of aging-induced mitochondrial DNA mutation enhanced mitochondrial function as evidenced by increased oxygen consumption. Our previous melatonin studies also led us to hypothesize that caloric restriction and the hormone melatonin would reverse brain mitochondrial dysfunction in animal …

The Continuum Of Ethno-Racial Socialization: Learning About Culture And Race In Middle-Class Latina/O Families, Maria D. Duenas

USF Tampa Graduate Theses and Dissertations

This thesis examines the discursive messages and specific practices that Latino families use to transmit messages about culture, race, and racism. Scholars have not fully explored the complexity and range of practices and discourses that are involved in Latinos’ ethno-racial socialization. The use of the phrase “ethno-racial socialization” is important because it combines the concepts of racial socialization and ethnic socialization in an effort to account for how the lived experiences of Latinos who mostly think of themselves as a racial group, are treated as one race, and thus, discuss race with family members. This research explores this process using …

Genomic Aberrations At The 3q And 14q Loci: Investigation Of Key Players In Ovarian And Renal Cancer Biology, Punashi Dutta

USF Tampa Graduate Theses and Dissertations

Genomic aberrations are primary contributors to the pathophysiology of cancer [11]. Dysregulated expression of genes located within these aberrations are important predictors of chemoresistance, disease prognosis, and patient outcome [12]. This dissertation is focused on understanding the regulation and/or functions of specific genes located at dysregulated genomic regions such as 3q26 and 14q32 in the biology of ovarian and renal cancer, respectively.

Serous epithelial ovarian cancer (EOC) manifest amplification at the 3q26.2 locus [2], an observation consistent with the cancer genome atlas (TCGA) [13]. The most amplified gene in this region is EVI1 which has been extensively studied in hematological …

The Effects Of Supplemented Metabolites On Lifespan And Stress Response Pathways In Caenorhabditis Elegans, Clare B. Edwards

USF Tampa Graduate Theses and Dissertations

Understanding how metabolites contribute to anaplerosis, antioxidant effects, and hormetic pathways during aging is fundamental to creating supplements and dietary habits that may decrease age-associated disease and decline, thus improving the quality of life in old age. In order to uncover metabolic pathways that delay aging, the effects of large sets of metabolites associated with mitochondrial function on lifespan were investigated.

Malate, the tricarboxylic acid (TCA) cycle metabolite, increased lifespan and thermotolerance in C. elegans. Addition of fumarate and succinate also extended lifespan and all three metabolites activated nuclear translocation of the cytoprotective DAF-16/FOXO transcription factor and protected from paraquat-induced …

Mmv Malaria Box Activity Screening In Dormant Plasmodium Falciparum Phenotypes, Sandra Galusic

USF Tampa Graduate Theses and Dissertations

The causative agent of malignant tertian malaria, Plasmodium falciparum undergoes an arrested growth phenotype of its erythrocytic stage when under drug-stress. Recent artemisinin treatment failures seem to be indicative of such induction followed by recrudescence rather than actual therapeutic failure. Likewise, P. vivax hypnozoites are the prototypic dormants and the latent infections for which they are responsible prove most difficult to treat. Dihydroartemisinin, an artemisinin-derivative, can be used to exploit this mechanism by inducing a dormant state in ring-stage P. falciparum parasites and in turn, their recovery may be used as a screening period for compounds that inhibit or foster …

A Numerical And Experimental Investigation Of Flow Induced Noise In Hydraulic Counterbalance Valves, Mutasim Mohamed Elsheikh

USF Tampa Graduate Theses and Dissertations

The main objective of this study is to explore the complex fluid flow phenomena that result in the generation of a high frequency noise in counterbalance valves through an experimental and numerical investigation of the flow. Once the influence of the different components involved in noise generation is established, a secondary objective is the introduction of design modifications that eliminate the undesired effect without altering the operation envelope or the performance of the valve.

A hydraulic test bench was used to carry out an experimental investigation of the noise generation process. A computer based data acquisition system was used to …

Iwitness And Student Empathy: Perspectives From Usc Shoah Foundation Master Teachers, Brandon Jerome Haas

USF Tampa Graduate Theses and Dissertations

This qualitative interview study explores the perceptions of five USC Shoah Foundation Master Teachers who integrate IWitness in teaching about the Holocaust. The study focused on the perceptions of teachers as their students interact with survivor and witness testimony in IWitness as well as how IWitness provides a framework for moral education in comparison to other primary sources. Data gleaned from this study demonstrates the influence of personalized learning through testimony-based education on the development of empathy in secondary students. As IWitness is a new resource that engages students with Holocaust survivor testimony in innovative ways, this study fills a …

The Role Of The Interruption In Young Adult Epistolary Novels, Betty J. Herzhauser

USF Tampa Graduate Theses and Dissertations

Within the genre of young adult literature, a growing trend is the use of epistolary messages through electronic methods between characters. These messages are set apart from the formal text of the narrative of the novel creating a break in the text features and layout of the page. Epistolary texts require a more sophisticated reading method and level of interpretation because the epistolary style blends multiple voices and points of view into the plot, creating complicated narration. The reader must navigate the narrator’s path in order to extract meaning from the text. In this hermeneutic study, I examined the text …

Heart Of The Beholder: The Pathos, Truths And Narratives Of Thermopylae In _300_, James Christopher Holcom

USF Tampa Graduate Theses and Dissertations

This thesis argues that critical understanding of historical narratives needn’t be limited to cold, clinical applications of logic and reason. By doing a close textual reading of Frank Miller and Lynn Varley’s graphic novel, 300 and Zack Snyder’s 2007 film adaptation, I posit that critical analysis of popular narratives is better served when pathos takes a central role. Traditional rhetorical criticism tends to favor empirical evidence and fact over emotional, narrative truth. Yet, the writing, recounting and interpretation of history are more akin to arts than sciences. Historical narratives are subject to the same influences and techniques that make poetry, …

Tampa Electric Company's Big Bend Utility Plant In Hillsborough County, Florida: A Case Study, Lynne M. Hodalski-Champagne

USF Tampa Graduate Theses and Dissertations

This is an in-depth analysis of coal fire burning power plants, their effects on human health and the environment. It also employed case study data from Tampa Electric Company’s Big Bend facility to examine environmental infractions at that facility. Tampa Electric Company’s Big Bend Utility Plant, violated the Clean Air Act, which led to a lawsuit filed by the Department of Justice on behalf of the United States Environmental Protection Agency and the Florida Department of Environmental Protection in 1997. This case study details the lawsuit, and subsequent settlement as well as Tampa Electric Company’s record of compliance since 2000. …

Evidence Of Things Not Seen: A Semi-Automated Descriptive Phrase And Frame Analysis Of Texts About The Herbicide Agent Orange, Sarah Beth Hopton

USF Tampa Graduate Theses and Dissertations

From 1961 to 1971 the United States and the Republic of South Vietnam used chemicals to defoliate the coastal and upload forest areas of Viet Nam. The most notorious of these chemicals was named Agent Orange, a weaponized herbicide made up of two chemicals that, when combined, produced a toxic byproduct called TCDD-dioxin. Studied suggest that TCDD-dioxin causes significant human health problems in exposed American and Vietnamese veterans, and possibly their children (Agency, U.S. Environmental Protection, 2011). In the years since the end of the Vietnam War, volumes of discourse about Agent Orange has been generated, much of which is …

Tornado Fatalities: An In-Depth Look At Physical And Societal Influences, Heather Joann Key

USF Tampa Graduate Theses and Dissertations

The purpose of this study is to model and determine significant predictors of tornado death index values, and to investigate these significant predictors and what makes people vulnerable to tornado fatalities through expert interviews. This study also provides an understanding of the study participant’s perceptions of their county’s vulnerability to tornado fatality and demonstrates a true integration of methods and fields by studying geographic, meteorological, and sociological phenomena by use of quantitative and qualitative methods. The study consists of two parts: 1) A quantitative exploration of variables hypothesized to predict Tornado Death Index (TDI) values, 2) A qualitative investigation to …

Looking At Levels Of Medicalization In The Institutional Narrative Of Substance Use Disorders In The Military, Chase Landes Mccain

USF Tampa Graduate Theses and Dissertations

The purpose of this research is to examine the institutional narrative of substance use disorders (SUDs) in the U.S. military and the extent to which it reflects the medicalization process. Three general research questions guided my analysis of the narrative surrounding SUDs in the military: (1) How does the military characterize the problems and resolutions of SUDs? (2) How and to what extent does this narrative reflect medicalization? (3) What are the limitations inherent in the institutional narrative of SUDs in the military? In order to address these questions, I draw on three conceptual lenses: (1) The work of Loseke …

The Triumvirate Of Intersectionality: A Case Study On The Mobilization Of Domésticas In Brazil, Kristen Lei Nash

USF Tampa Graduate Theses and Dissertations

In this thesis, I look at the mobilization of the domestic workers in Brazil as a social movement. In Brazil, the domestic workers have managed to organize continuously for over eight decades using both informal and formal mechanisms to connect workers all over the country in unique ways. By viewing these women and the ways in which they have organized in the framework of a social movement, we can begin to identify their repertoires of contention and how those repertoires have contributed to the successes of the movement. In order to guide this investigation, I ask, how has the doméstica …

Women’S Use Of And Decision‐Making Regarding Geo‐Social Networking Applications To Arrange Sexual Experiences, Shireen Mary Noble

USF Tampa Graduate Theses and Dissertations

The purpose of this thesis was to learn more about geo-social networking applications and how they are used by women to meet sexual partners. Currently, there are no known studies that have looked at heterosexually-oriented geo-social networking applications or at the way women have used these apps for heterosexual sexual encounters. This thesis attempts to begin to bridge this gap in the literature.

For the first study, apps were selected based on their appearance in popular media articles about dating applications. Results generally related to online safety concerns, while occasional features were related to sexual safety concerns. Communication options were …

Undergraduate College Students’ Attitudes About Internet-Based Mental Health Interventions, Kathleen Palmer

USF Tampa Graduate Theses and Dissertations

Millennial-aged young adults, often referred to as “digital natives,” comprise the typical college-age population, and there has been a growing number college students at risk for mental health problems (Mowbray, Mandiberg, Stein, Kopels, Curlin, Megivern, Strauss, Collins & Lett, 2006; Eisenberg, Gollust, Golberstein & Hefner, 2007). Suicide is the second leading cause of death among college students (Suicide Statistics, 2014); however, their rate of utilizing mental heath counseling is decreasing. Providing the types of mental health services college students are likely to use can mitigate factors thought to impede their use (e.g., stigma, anonymity, confidentiality), as well as help improve …

Feeling At Home With Grief: An Ethnography Of Continuing Bonds And Re-Membering The Deceased, Blake Paxton

USF Tampa Graduate Theses and Dissertations

Bereavement scholars Silverman, Nickman, and Klass (1996) have argued that rituals to continue a relationship with the deceased do not have to be considered pathological in nature. Since their work, scholars have offered specific strategies for the bereaved to actively construct a bond after death, including telling stories about those who have died, having imagined conversations with the deceased, celebrating their birthdays and anniversaries, and reviewing artifacts that represent or once belonged to them (among other strategies). Hedtke and Winslade (2004) call these “re-membering” processes by which the deceased can regain active membership in their loved ones lives. This dissertation …

Understanding The Role Of Colloidal Particles In Electroporation Mediated Delivery, Alisha Peterson

USF Tampa Graduate Theses and Dissertations

Electroporation (EP) is a physical non-viral technique used to deliver therapeutic molecules across the cell membrane. During electroporation an external electric field is applied across a cell membrane and it causes pores to form. These pores then allow the surrounding media containing the therapeutics to diffuse across the membrane. This technique has been specifically studied as a promising gene and drug delivery system. Colloidal particles have also proven to be promising for a variety of biological applications including molecular delivery, imaging, and tumor ablation, due to their large surface area and tunable properties. In more recent years researchers have explored …

Ecological Sanitation In Uganda: Promotion Through Demonstration Facilities And Potential For Ascaris Reduction By Free Ammonia Inactivation Using Stored Urine, John Thomas Trimmer

USF Tampa Graduate Theses and Dissertations

As Uganda works to transform itself into an industrialized, middle-income country in the coming decades, the country is faced with a number of problematic trends that could hinder this transition. High population growth and urbanization are quickly forcing small towns to deal with issues of limited space and the aesthetic conditions within sanitation systems, while declining soil fertility in surrounding rural areas calls into question the future nutritional security of the growing population. Ecological Sanitation (Eco-San) systems, which are designed to recover nutrients from human excreta, may help to address these trends.

Improved sanitation coverage in Uganda is currently estimated …

Metallic Encapsulation For High Temperature (>500 °C) Thermal Energy Storage Applications, Abhinav Bhardwaj

USF Tampa Graduate Theses and Dissertations

Deployment of high temperature (>500 °C) thermal energy storage in solar power plants will make solar power more cost competitive and pave the way towards a sustainable future. In this research, a unique metallic encapsulation has been presented for thermal energy storage at high temperatures, capable of operation in aerobic conditions. This goal was achieved by employing low cost materials like carbon steel. The research work presents the unique encapsulation procedure adopted, as well as various coatings evaluated and optimized for corrosion protection. Experimental testing favored the use of 150 μm of nickel on carbon steel for corrosion protection …

Exploring The Role Of Intracellular Aminopeptidases In Staphylococcus Aureus Pathogenesis, Devon Nicole Marking

USF Tampa Graduate Theses and Dissertations

Staphylococcus aureus is a remarkably pathogenic bacterium that is widely prevalent among the human population. It is the leading agent of skin and soft tissue infections, and is also responsible for causing an array of severe and life threatening diseases. The invasiveness of the pathogen, coupled with increasing antibiotic resistance seen for S. aureus infections, makes this bacterium a prominent public health concern. The extended pathogenicity of S. aureus is largely due to its repertoire of virulence factors, which are typically characterized by being bound to the cell wall, or secreted into the extracellular environment. Previously, our lab identified a …

A Composite Spatial Model Incorporating Groundwater Vulnerability And Environmental Disturbance To Guide Land Management, Johanna L. Kovarik

USF Tampa Graduate Theses and Dissertations

Research has long recognized and studied the dynamics of groundwater processes. More recently, groundwater dependent ecosystems (GDEs) are being recognized for their diversity and vulnerability to anthropogenic impact. Groundwater in karst landscapes presents a distinctive situation where flow through the subsurface often moves rapidly on the scale of days and weeks as opposed to years or millennia in other systems. This distinctive situation of karst systems and their vulnerability to human impacts necessitate an integrated and multifaceted approach for the management of these important resources. However, development of such an approach is complicated by the difficulty of obtaining detailed data …

Pathogenic Policy: Health-Related Consequences Of Immigrant Policing In Atlanta, Ga, Nolan Sean Kline

USF Tampa Graduate Theses and Dissertations

Multilayered immigration enforcement regimes comprising state and federal statutes and local police practices demand research on their social and health-related consequences. This dissertation explores the multiple impacts of immigrant policing: sets of laws and police activities that make undocumented immigrants more visible to authorities and increase their risk of deportation. Examining immigrant policing through a multi-sited framework and drawing from principles of engaged anthropology, findings from this dissertation suggest how immigrant policing impacts undocumented immigrants' overall wellbeing, health providers' professional practice, and reveals troubles with safety net medical care. Interviews and participant observation experiences suggest how immigrant policing perpetuates a …

Student-Teacher Interaction Through Online Reflective Journals In A High School Science Classroom: What Have We Learned?, Megan Elizabeth Ehlers

USF Tampa Graduate Theses and Dissertations

Three challenges in current secondary school science classrooms are (a) meaningful integration of technology, (b) integration of reading and writing in content courses, and (c) differentiation of instruction to meet individual student needs in courses. This is an exploratory study of an urban, high school marine science course in which a teacher added communication with her students via asynchronous online journals. This intervention was intended to enable the teacher to understand how students were constructing knowledge and their understanding of marine science topics. Data included journal postings from all students and the teacher throughout the semester, as well as the …

Analytics And Healthcare Costs (A Three Essay Dissertation), Lina Bouayad

USF Tampa Graduate Theses and Dissertations

Both literature and practice have looked at different strategies to diminish healthcare associated costs. As an extension to this stream of research, the present three paper dissertation addresses the issue of reducing elevated healthcare costs using analytics. The first paper looks at extending the benefits of auditing algorithms from mere detection of fraudulent providers to maximizing the deterrence from inappropriate behavior. Using the structure of the physicians' network, a new auditing algorithm is developed. Evaluation of the algorithm is performed using an agent-based simulation and an analytical model. A case study is also included to illustrate the application of the …

Hpv Vaccine Decision-Making Among Male Sexual Minorities: An Integrative Theoretical Framework For Vaccine Promotion, Christopher W. Wheldon

USF Tampa Graduate Theses and Dissertations

Men who have sex with men (MSM) are at increased risk of anal cancer as a result of anal HPV infection. Routine HPV vaccination is recommended for all MSM up through age 26; however, vaccine uptake among this population is low. The Integrative Model of Behavioral Prediction (IM) was used to identify, describe, and explain psychosocial factors related to HPV vaccine decision-making for young MSM. A sequential mixed-methods approach consisting of semi-structured interviews, a quantitative survey, and a qualitative open-ended survey was used to address the following aims: (1) Determine salient outcome, normative, efficacy, and control beliefs related to HPV-vaccination …

The Action Cycle/Structural Context Framework: A Fisheries Application, D. G. Webster

Dartmouth Scholarship

There is a growing consensus that environmental governance is a wicked problem that requires understanding of the many linkages and feedbacks between human and natural systems. Here, I propose an action cycle/structural context (AC/SC) framework that is based on the concept of responsive governance, in which individuals and decision makers respond to problems rather than working to prevent them. By linking agency and structure, the AC/SC framework points out two key problems in the realm of environmental governance: the profit disconnect, whereby economic signals of environmental harm are dampened by endogenous or exogenous forces, and the power disconnect, whereby those …

Nonlinear Analysis And Digital Pre-Distortion Of The Sc-Fdma Signals In Lte Uplink System, Changwen Zhu

Dissertations and Theses

Single-carrier frequency division multiple access (SC-FDMA) has become a popular alternative to orthogonal frequency division multiple access (OFDM) in multi-user communication on LTE uplink systems. This is primarily due to the low peak-to-average power ratio (PAPR) of SC-FDMA compared to that of OFDM. Long-term evolution (LTE) uses SC-FDMA on the uplink to exploit this PAPR advantage to reduce transmit power amplifier (PA) back-off in mobile user terminals. However, the latest generation of communication systems requires high power efficiency and a large quantity of capacity in transmitting mobile data, bringing out some other critical problems: 1) the nonlinearity of Radio Frequency …