Digital Commons Network^™

Hiv-1 Adaptation To T-Cell Responses During Infection, Victor Yimin Du

All ETDs from UAB

recommended vitamin D intake, and perceived barriers to adequate calcium intake

Akhuwat Issue No. 98, Aga Khan University

AKHUWAT Newsletters

• Dr Sadia Rasul receives Order of British Columbia
• Dr Bhutta receives CPBC's Professional of the Year Award
• AKU and DW sign a memorandum of understanding to develop world-class professional development programmes
• Farewell to Dr Greg Moran and Dr Yasmin Amarsi
• A time to bid farewell
• Leadership workshop to accelerate reduction of maternal and newborn deaths
• Africa's First Ladies visit AKUH Heart and Cancer Centre
• Enhancing Research Ethics Committee Reaccredited
• First PGME Open Day in East Africa
• Grid Station to meet University's future needs
• AKU-EB Middle School Programme Developing 21st Century Skills
• AKU-EB's unwavering commitment to education
• The Spirit of Service! …

Bcla As An Adjunct To Current Bacillus Anthracis Vaccination And Therapy Protocols, Juan Bosco Rodriguez Barrantes

All ETDs from UAB

Bacillus anthracis (Ba) is a Gram,-positive zoonotic bacterium that causes inhala- tional, cutaneous and intestinal Anthrax Disease. Although the infectious form of Ba is its endospore (spore), only the tripartite toxin-producing vegetative bacteria causes disease pathology. While current vaccination strategies target the Protective Antigen (PA) com- ponent of this toxin, vaccine-elicited immunity to the spore form of Ba is lacking. How- ever, vaccines targeting spore components of Ba have the potential to neutralize the in- fectious form of Ba. We sought to determine if antibodies elicited through immunization with the main antigenic component of the spore surface- Bacillus collagen-like protein …

Emergency Takings, Brian Angelo Lee

Michigan Law Review

Takings law has long contained a puzzle. The Fifth Amendment to the U.S. Constitution requires the government to pay “just compensation” to owners of private property that the government “takes.” In ordinary circumstances, this requirement applies equally whether the property is confiscated or destroyed, and it also applies to property confiscated in emergencies. Remarkably, however, courts have repeatedly held that if the government destroys property to address an emergency, then a “necessity exception” relieves the government of any obligation to compensate the owner of the property that was sacrificed for the public good. Although the roots of this startling principle …

Ethnic Disparities In The Reproductive And Sexual Health Screening Practices Of Ecuadorian Women, Ruth Castillo

Open Access Theses & Dissertations

Background: Evidence suggests that reproductive and other health disparities disproportionately affect Indigenous and Afro-Hispanic ethnic minority groups throughout the Latin American and Caribbean (LAC) region. Factors associated with reproductive health disparities in LAC countries include low social economic status (SES), poor health care access, racial and gender discrimination, machismo, health beliefs and low perceived risk for breast cancer, cervical cancer, sexual transmitted infections (STIs) and other conditions. Preventive measures such as health education and early screening is vital for disease prevention and detection. However, screening rates for breast cancer, cervical cancer, and STIs are lower in most LAC countries compared …

Identification Of Leishmania Spp. And T. Cruzi Parasites In Stray Felines In El Paso, Texas Through Polymerase Chain Reaction, Patricia Isabela Gonzalez

Open Access Theses & Dissertations

Chagas disease and leishmaniasis are considered neglected parasitic diseases, and although dogs are considered the main domestic reservoirs, infected cats have recently been found in endemic areas in several countries and became a public health concern. These diseases can be transmissible to other animals and are known to infect humans as well. Natural infection of cats by Leishmania spp. and Trypanosoma cruzi has been demonstrated in several European, Latin American, and Asian countries. A recent field study found dogs and wild animals tested positive for both Leishmania and Trypanosoma cruzi in the El Paso, Texas Region. Cats, however, have not …

Studying Cellular And Molecular Interaction With Two-Photon Fluorescence Microscopy, Arifur Rahaman

Open Access Theses & Dissertations

Advances in microscopy and fluorescent probes provide new insight into studying cellular and molecular interactions. Two-photon fluorescence microscopy is one of the most important recent inventions in cellular and molecular study. This technology enables noninvasive study at cellular and molecular levels in three dimensions with submicrometer resolution. Two-photon excitation of fluorophores results from the simultaneous absorption of two photons. This excitation process has a number of unique advantages, such as reduced specimen photodamage and enhanced penetration depth. In this study, we used our two-photon microscope to observe predatorial behavior of fast moving bacterivorous marine organism Cafeteria roenbergensis and quantify Mycobacterium …

Task Shifting To Nursing For A Sustainable Staffing Model Of School-Based Health Center In Under-Resourced Areas, Laura Lee Setliff Whittaker

Yale School of Nursing Digital Theses

Background: There is a well-established link between health and education (Bundy and Guyatt 1996). An efficient and cost effective way to increase access to care is through school-based services.

Methods: The purpose of this study is to develop a sustainable staffing model of a school-based health center in a country with limited health care resources. Two specific steps towards developing such a model are: (1) to critically review and analyze the evidence in the literature supporting an expanded nursing care practice model for the school-based health center, and (2) to merge the literature review findings with participatory field notes taken …

Saccharomyces Boulardii And Bismuth Subsalicylate As Low-Cost Interventions To Reduce The Duration And Severity Of Cholera, Johnathan Sheele, Jessica Cartowski, Angela Dart, Arjun Poddar, Shikha Gupta, Ajay Gupta

Computer Science Faculty Publications

We conducted a randomised single-blinded clinical trial of 100 cholera patients in Port-au-Prince, Haiti to determine if the probiotic Saccharomyces cerevisiae var. boulardii and the anti-diarrhoeal drug bismuth subsalicylate (BS) were able to reduce the duration and severity of cholera. Subjects received either: S. boulardii 250 mg, S. boulardii 250 mg capsule plus BS 524 mg tablet, BS 524 mg, or two placebo capsules every 6 hours alongside standard treatment for cholera. The length of hospitalisation plus the number and volume of emesis, stool and urine were recorded every 6 hours until the study subject was discharged (n=83), left against …

Restraint Stress Alters Pro-Inflammatory Cytokine Expression And Increases Superoxide Dismutase In The Rat Hypothalamus And Hippocampus, Kristina Isabel Barron

Open Access Theses & Dissertations

The impact of restraint stress on changes in cytokine expression and oxidative stress markers in hippocampal (Hipp) and hypothalamic (Hyp) regions of the brain, were assessed in a rat model. In addition, immune and inflammatory variables were assessed in peripheral blood. Male Wistar rats were acutely (30 min) or repeatedly (30 min/d for 14 consecutive days) exposed to a restraint stress, or maintained as non-stressed controls. At the time of sacrifice, whole blood and brain tissues enriched in Hyp or Hipp regions were collected; blood was collected with EDTA as an anticoagulant and centrifuged to yield plasma. All tissue samples …

Serine Palmitoyltransferase And Giardial Encystation, Monica Delgado

Open Access Theses & Dissertations

Giardia lamblia is a flagellated protozoan parasite responsible for chronic diarrheal disease in humans and animals. Giardia has minimal lipid synThesis machinery and the majority of its lipids are obtained from the small intestine of humans where the trophozoites colonize. Recent results from our laboratory indicate that sphingolipids (SLs) are also important for Giardia and they regulate encystation and cyst production. Only five SL genes are present in this parasite and they are differentially regulated during encystation. In my studies, I found that giardial serine palmitoyltransferase (gSPT) activity is upregulated in encysting cells, suggesting it might play an important role …

The Meteorological Conditions Affecting The Propagation Of Respiratory Diseases, Rosette Maria Gonzalez

Open Access Theses & Dissertations

Valley Fever, also known as Coccidioidomycosis, is endemic of the US Southwest. In 2012 this disease was the second most-reported disease in Arizona, according to the New Yorker January 2014 edition. The Paso Del Norte Region (PdN) is a semi-arid region with significant agricultural acreage and Valley Fever is known to be a threat in this region. It is our goal in this Thesis work to apply an existing PBL Height Program (e.g., Liu, et.al.) to the Paso Del Norte Region (PdN) to study the effect it has in the transmission of Valley Fever. Obtaining the PBLH as it evolves …

Bounded Rationality In Decision Making Under Uncertainty: Towards Optimal Granularity, Joseph Anthony Lorkowski

Open Access Theses & Dissertations

Starting from well-known studies by Kahmenan and Tversky, researchers have found many examples when our decision making seems to be irrational. We show that this seemingly irrational decision making can be explained if we take into account that human abilities to process information are limited. As a result, instead of the exact values of different quantities, we operate with granules that contain these values. On several examples, we show that optimization under such granularity restriction indeed leads to observed human decision making. Thus, granularity helps explain seemingly irrational human decision making.

Similar arguments can be used to explain the success …

Exploratory Study Of Seasonal Indoor Bioaerosols And The Associated Health Outcomes In Low-Income Communities In El Paso, Texas, 2014, Eric Martinez

Open Access Theses & Dissertations

Introduction: Structural degradation, moisture levels, and building failures can influence poor indoor air quality and create a good environment for bioaerosols. People spend much of their time indoors and can potentially be exposed to these bioaerosols. Several studies have correlated exposures to high concentrations of bioaerosols to various health problems, severe allergies, asthma, or other respiratory illness. Objectives: The objectives of this study are to describe bioaerosol concentrations, meteorological factors, and health outcomes during the summer and fall seasons; characterize bioaerosols (bacteria and fungi) collected in low-income communities; compare bioaerosol concentrations by season; compare meteorological factors to bioaerosol concentrations by …

Detecting Enzootic Leishmaniasis And American Trypanosomiasis In Stray Dogs In El Paso County, Texas And The Potential For Autochthonous Transmission To Humans, Evan James Kipp

Open Access Theses & Dissertations

Cutaneous leishmaniasis and Chagas disease (American trypanosomiasis) are two vector-borne, protozoal zoonoses whose emergence into the southern United States is a public health problem of increasing significance. Cutaneous leishmaniasis is caused by several species of intracellular protozoa in the genus Leishmania and is most often characterized by the formation of large, ulcerative skin lesions that can result in considerable scarring and permanent disfigurement. Infection with Leishmania is prevalent throughout the world in tropical and sub-tropical regions and in areas where people are regularly exposed to the hematophagous sand fly vectors that transmit the disease. Chronic infection with Trypanosoma cruzi, the …

Esophageal Obstruction In Water Buffalo (Bubalus Bubalis): A Retrospectivestudy Of 44 Cases (2006?2013), Mohamed Marzok, Alaa Moustafa, Sabry El-Khodery, Kerstin Müller

Turkish Journal of Veterinary & Animal Sciences

The purposes of this study were to report the types, causes, and clinical findings of esophageal obstructions in water buffaloes and to verify the role of radiography in diagnosis as well as the evaluation of the proposed treatment management and outcome after treatment. Forty-four buffaloes with esophageal obstruction were used in the study. Esophageal obstruction was significantly more frequent in females than males. Buffaloes at 1-3 years of age were significantly more liable to the disease than those of other ages. Complete obstruction was more frequent than partial obstruction. Intraluminal obstruction was more prevalent than extraluminal. Obstruction at the cervical …

The Recovery Of Gut Barrier Function With Selenium Rich Diet In Acute Dss-Induced Colitis, Sarah Depaepe

Theses and Dissertations

Background: Acute Dextran Sodium Sulfate (DSS)-induced colitis is an inflammatory ailment limited to the colon. It works to destroy the morphology and gut barrier goblet and epithelial cells that aid in providing homeostasis. Selenium (Se) is an essential micronutrient that has anti-inflammatory and antioxidant properties and is known to play a role in reducing inflammation in areas elsewhere in the body. The current study is focused on how Se alters gut barrier permeability and functionality related to the recovery of tight junction regulation and mucin secretion. Methods: C57BL/6 mice were randomly placed into control (normal water) and 2% DSS water …

Predictors And Profiles Of Antiretroviral Therapy Adherence Among African-American Adolescents And Young Adult Males Behaviorally-Infected With Hiv: A Classification Tree Analysis Approach, Israel Moses Gross

Dissertations

Adherence to antiretroviral therapy is crucial for thwarting disease progression and reducing secondary transmission, yet HIV+ youth struggle with adherence. The highest rates of new HIV infections occur in young African American men (YAAM), thus understanding reasons for non-adherence in this group is critical. Reasons for non-adherence can be complex and multifactorial, and innovative methods of exploration are needed for advancing prevention and treatment efforts. A sample of 387 HIV+ YAAM who reported currently taking HIV medications were selected from a cross-sectional assessment of 2,226 HIV+ youth from sites within the Adolescent Trials Network for HIV/AIDS Interventions (ATN) from 2009-2012 …

Paradoxes In Physical Health, Robert Crosnoe, Aida Ramos-Wada, Claude Bonazzo

Faculty Publications - Department of Sociology

This chapter reviews the evidence from social science and medical research that sheds light on this potential immigrant paradox in health -- when, where, and for whom it holds or does not apply. In doing so, two important points need to be kept in mind. First, the existence of the paradox does not necessarily mean that immigrants are doing than everyone else. Rather, it means that they are doing better than social and economic positions suggest that they should be. Second, paradox may apply in general but not hold in specific domains of health for certain subgroups or at certain …

The Effects Of Stress And Alcohol On Hiv-1 Latency, Sarah Ilene Talley

Master's Theses

A major barrier to HIV eradication is the persistence of latent viral reservoirs that exist despite antiretroviral therapy. In order to develop effective therapeutics, a comprehensive understanding of latency and factors driving the formation of the latent reservoir is needed. As stress and alcohol are common comorbidities associated with HIV infection, the goal of this research was to determine how stress and alcohol could affect HIV-latency. Specifically, we hypothesized that the ability of latent proviruses to be reactivated by “shock and kill” approaches would be altered by glucocorticoid and ethanol treatments, and prolonged ethanol exposure would affect the size of …

Characterization Of Exopolysaccharide (Eps) Produced By Bacillus Subtilis Mutants, Alexander Argianas

Master's Theses

Bacillus subtilis is a probiotic bacterium that can protect against the murine-

specific attaching and effacing (A/E) pathogen Citrobacter rodentium. C. rodentium displays many features observed with the human pathogen enteropathogenic E. coli (EPEC). Protection by B. subtilis is mediated by exopolysaccharide (EPS), which is encoded by the epsA-O operon that contains 15 genes. Disruption of one of these genes, epsH, which appears to encode a putative glycosyltransferase, leads to the loss of protective capacity by B. subtilis and by EPS. EPS is a major component of the B. subtilis biofilm and the ΔepsH mutant is unable to form a …

Role Of Heat Shock Proteins In Maintenance Of The Gut Barrier Following Burn Injury, Abigail Rhea Cannon

Master's Theses

Introduction: Burn injury remains a prominent clinical problem. Patients suffering from burns often succumb to secondary infectious complications leading to sepsis and widespread tissue injury ending in Multiple Organ Dysfunction. However, the mechanism behind the onset of these extraneous symptoms following burn injury is not fully understood. The integrity of the gut barrier is of critical importance as it harbors the largest bacterial reservoir in the body. Following burn injury, we observed a breakdown of the gut barrier resulting in increased gut leakiness and bacterial translocation. Under homeostatic conditions, heat shock proteins (HSPs) stabilize tight junction proteins. Particularly, HSP72 is …

Tcr Modifications To Enhance Expression, Chain Pairing, And Antigen Recognition For Adoptive T Cell Transfer, Kendra Foley

Master's Theses

T cell receptor (TCR) gene modified T cells for adoptive T cell transfer therapy have been shown to have clinical success in treating melanoma and other malignancies by redirecting the specificity of peripheral blood lymphocytes (PBL) to recognize tumor and/or viral associated antigens of choice. One of the challenges in using TCR gene modified T cells is the potential mispairing between endogenous and introduced alpha and beta TCR chains, allowing for unwanted off-target reactivity, autoimmunity, or impaired therapeutic efficacy. One approach to augment proper TCR chain pairing and to enhance T cell function involves the modification of the introduced TCR …

Human Metapneumovirus Is Capable Of Entering Cells By Fusion With Endosomal Membranes, Reagan G. Cox, Bernardon A. Mainou, Monika Johnson, Andrew K. Hastings, Jennifer E. Schuster, Terence S. Dermody, John V. Williams

Manuscripts, Articles, Book Chapters and Other Papers

© 2015 Cox et al. Human metapneumovirus (HMPV), a member of the Paramyxoviridae family, is a leading cause of lower respiratory illness. Although receptor binding is thought to initiate fusion at the plasma membrane for paramyxoviruses, the entry mechanism for HMPV is largely uncharacterized. Here we sought to determine whether HMPV initiates fusion at the plasma membrane or following internalization. To study the HMPV entry process in human bronchial epithelial (BEAS-2B) cells, we used fluorescence microscopy, an R18-dequenching fusion assay, and developed a quantitative, fluorescence microscopy assay to follow virus binding, internalization, membrane fusion, and visualize the cellular site of …

The Fuzzin' Facts, Rebel Alliance, Mitch Ohnstad, Rebekka Erks, Erin Prohaska, Ryan Mueksch, Rachel Foehlinger, Laura Bergen, Sean Davis

College of Journalism and Mass Communications: Student Advertising Projects

The challenge for this campaign is to expose the facts about sexual health and safe sex practices in a light-hearted manner in order to make it a more comfortable topic to talk about so that more people understand the need to get the facts, get tested and get involved to help prevent the spread of sexually transmitted diseases in Nebraska.

Characterization Of Oral Microbiota In Xerostomic Versus Non-Xerostomic Volunteers And In Daily Samples Following Standard Oral Hygiene Practices, Chelsea Myers

Wayne State University Theses

A thesis presented on the characterization of oral microbiota in xerostomic versus non-xerostomic volunteers and in daily samples following standard oral hygiene practices. Xerostomia is a difficult and burdensome disease that can be very difficult to diagnose. Understanding the oral microbiota between these diseased and healthy (non-xerostomic) can give us great insight on new treatments and/or prevention. Goals of the study included determining whether there substantial differences in oral microbial populations between the two groups, and whether varying nightly oral hygiene practices had an impact on next-morning oral microbiota titers or composition. Microbial loads were determined by qPCR using broad-spectrum …

Meningitis Or Septicaemia In A Backpacker?, Sascha Fulde, Gordian Fulde

Medical Papers and Journal Articles

Negative blood test results for meningitis but positive for Staphylococcus aureus in a young patient with suspected meningitis and a recent joint injury led to a diagnosis of staphylococcal septicaemia with septic arthritis as the source of the infection.

Immunotherapy Of Cancer: Reprogramming Tumor/Immune Cellular Crosstalk To Improve Anti-Tumor Efficacy, Kyle K. Payne

Theses and Dissertations

Immunotherapy of cancer has been shown to be promising in prolonging patient survival. However, complete elimination of cancer and life-long relapse-free survival remain to be major challenge for anti-cancer therapeutics. We have previously reported that ex vivo reprogramming of tumor-sensitized immune cells by bryostatin 1/ionomycin (B/I) and the gamma-chain (γ-c) cytokines IL-2, IL-7, and IL-15 resulted in the generation of memory T cells as well as CD25+ NKT cells and CD25+ NK cells. Adoptive cellular therapy (ACT) utilizing these reprogrammed immune cells protected FVBN202 mice from tumor challenge, and overcame the suppressive functions of myeloid-derived suppressor cells (MDSCs). We then …

Mast Cells In Kainate Receptor Knockout Mice, Andrea J. Elkovich

Theses and Dissertations

Kainate receptor knockout mice have unique differences within their immune system. They exhibit an attenuated T_H2 branch, while maintaining a robust T_H1 response. Specifically, blocking the formation of functional kainate receptors affects mast cells and their related pathologies. While they seem to develop and activate normally in vivo and in vitro, KAR KO mast cells release more inflammatory mediators upon degranulation. These mice experience severe anaphylactic shock due to two compounding abnormalities. First, KAR KO mast cells release significantly more histamine in vivo upon IgE-mediated activation. Second, the animals over-respond to exogenous histamine with drastic …

Characterization Of Transfer Of The Mobile Genomic Island Encoding Methicillin Resistance Among Staphylococci, Melissa D. Ray

Theses and Dissertations

The gene encoding methicillin resistance in Staphylococcus aureus (MRSA) is carried in the chromosome on a large genomic island called SCCmec and is always inserted at the att site within orfX. SCCmec has been designated a mobile genetic element but a mechanism by which it moves among different strains and species of staphylococci has never been demonstrated. This work shows that bacteriophage 80α is capable of transducing SCCmec into a recipient cell, after which it can integrate into the bacterial chromosome via homologous recombination. More importantly, this work characterizes a conjugative mechanism of SCCmec transfer. …