Digital Commons Network^™

Experimental Evolution Of An Rna Virus In Wild Birds: Evidence For Host-Dependent Impacts On Population Structure And Competitive Fitness, Nathan D. Grubaugh, Darci R. Smith, Doug E. Brackney, Angela M. Bosco-Lauth, Joseph R. Fauver, Corey L. Campbell, Todd A. Felix, Hannah Romo, Nisha K. Duggal, Elizabeth A. Dietrich, Tyler Eike, Jennifer E. Beane, Richard A. Bowen, William C. Black, Aaron C. Brault, Gregory D. Ebel

Journal Articles: Epidemiology

Within hosts, RNA viruses form populations that are genetically and phenotypically complex. Heterogeneity in RNA virus genomes arises due to error-prone replication and is reduced by stochastic and selective mechanisms that are incompletely understood. Defining how natural selection shapes RNA virus populations is critical because it can inform treatment paradigms and enhance control efforts. We allowed West Nile virus (WNV) to replicate in wild-caught American crows, house sparrows and American robins to assess how natural selection shapes RNA virus populations in ecologically relevant hosts that differ in susceptibility to virus-induced mortality. After five sequential passages in each bird species, we …

Development Of A Serological Assay For The Sea Lion (Zalophus Californianus) Anellovirus, Zcav, Elizabeth Fahsbender, Karyna Rosario, John Cannon, Frances Gulland, Larry Dishaw, Mya Breitbart

Marine Science Faculty Publications

New diseases in marine animals are emerging at an increasing rate, yet methodological limitations hinder characterization of viral infections. Viral metagenomics is an effective method for identifying novel viruses in diseased animals; however, determining virus pathogenesis remains a challenge. A novel anellovirus (Zalophus californianus anellovirus, ZcAV) was recently reported in the lungs of captive California sea lions involved in a mortality event. ZcAV was not detected by PCR in the blood of these animals, creating the inability to assess the prevalence of ZcAV in live sea lions. This study developed an enzyme-linked immunosorbent assay (ELISA) to detect antibodies to …

Characterization Of A Novel Protease In Staphylococcus Aureus, Adam L. Johnson

Theses and Dissertations

A newly discovered cysteine protease, Prp, has been shown to perform an essential, site-specific cleavage of ribosomal protein L27 in Staphylococcus aureus. In Firmicutes and related bacteria, ribosomal protein L27 is encoded with a conserved N-terminal extension that must be removed to expose residues critical for ribosome function. Uncleavable and pre-cleaved variants were unable to complement an L27 deletion in S. aureus, indicating that this N-terminal processing event is essential and likely plays an important regulatory role. The gene encoding the responsible protease (prp) has been shown to be essential, and is found in all organisms …

High-Throughput Data Analysis: Application To Micronuclei Frequency And T-Cell Receptor Sequencing, Mateusz Makowski

Theses and Dissertations

The advent of high-throughput sequencing has brought about the creation of an unprecedented amount of research data. Analytical methodology has not been able to keep pace with the plethora of data being produced. Two assays, ImmunoSEQ and the cytokinesisblock micronucleus (CBMN), that both produce count data and have few methods available to analyze them are considered.

ImmunoSEQ is a sequencing assay that measures the beta T-cell receptor (TCR) repertoire. The ImmunoSEQ assay was used to describe the TCR repertoires of patients that have undergone hematopoietic stem cell transplantation (HSCT). Several different methods for spectratype analysis were extended to the TCR …

Microbiological Contamination Of Mobile Phones Of Clinicians In Intensive Care Units And Neonatal Care Units In Public Hospitals In Kuwait, Mohammed Heyba, Mohammad Ismaiel, Abdulrahman Alotaibi, Mohamed Mahmoud, Hussain Baqer, Ali Safar, Noura Al-Sweih, Abdullah Al-Taiar

Community & Environmental Health Faculty Publications

Background: The objective of this study was to explore the prevalence of microbiological contamination of mobile phones that belong to clinicians in intensive care units (ICUs), pediatric intensive care units (PICUs), and neonatal care units (NCUs) in all public secondary care hospitals in Kuwait. The study also aimed to describe mobile phones disinfection practices as well as factors associated with mobile phone contamination.

Methods: This is a cross-sectional study that included all clinicians with mobile phones in ICUs, PICUs, and NCUs in all secondary care hospitals in Kuwait. Samples for culture were collected from mobile phones and transported for microbiological …

Curriculum Change 2015-2016, Portland State University

Bulletin & Curriculum Change Documents

No abstract provided.

2014 Federal Legislative Review, Jessica Brockway

Animal Law Review

The 113th Congress escaped the designation of "least productive Congress in modern history" thanks to a particularly active lame duck session. During its session, the 113th Congress enacted 296 laws, 212 of which were "categorized ... as substantive " by the Pew Research Center, a nonpartisan "fact tank." This legislative activity, however, did not benefit animals. Of the pieces of proposed legislation discussed in this Review, none passed and, unless otherwise specified, all died in Committee.

Culturally Responsive Teaching In A War-Affected Area: Lesley University In Israel – 1979-2014, Vivien Marcow-Speiser, Samuel Schwartz

Journal of Pedagogy, Pluralism, and Practice

The article begins by assessing the theoretical issues raised in the literature regarding teaching in another culture and, in particular, teaching in a conflict zone. It then goes on to describe the specific conditions under which Lesley University’s program operated in Israel. A large portion of the article outlines the teaching and community outreach activities of Lesley University in Israel since 1979, and how they met the challenges of culturally-responsive teaching in a war-affected area, focusing on its work training therapists, educators and community activists, organizing conferences and launching community outreach programs. The article discusses issues such as preparing faculty …

Medicare And Medicaid Enrollment And Utilization Among Hiv-Infected And Uninfected Veterans In Va Care, Harry Chang

Yale Medicine Thesis Digital Library

Many veterans engaged in care with the Veterans Administration (VA) health system are also enrolled in Medicare and/or Medicaid and may receive care both inside and outside of the VA. Use of dual health systems has been associated with worse outcomes. Veterans with human immunodeficiency virus (HIV) may have different rates of Medicare and Medicaid enrollment, and may be at greater risk of poor outcomes related to non-VA use. This study compares the frequency of Medicare and/or Medicaid enrollment and factors associated non-VA use in an HIV-infected and uninfected population of veterans.

We used data from the VA and Center …

Racial/Ethnic Differences In The Presentation And Management Of Severe Bronchiolitis, Jonathan Santiago

Yale Medicine Thesis Digital Library

We examined if racial/ethnic differences exist in the presentation and management of severe bronchiolitis using a 16-center, prospective cohort study from 2007-2010. Children <2 years hospitalized with a diagnosis of bronchiolitis were included. A structured interview, chart review, and 1-week phone follow-up were completed. Multivariable logistic regression was used to examine the independent association between race/ethnicity and diagnostic imaging, treatment, management, discharge on inhaled corticosteroids, and bronchiolitis relapse. Among 2,130 patients, 818 (38%) were non-Hispanic white (NHW), 511 (24%) were non-Hispanic black (NHB), and 801 (38%) were Hispanic. Compared with all groups, NHB children were most likely to receive albuterol before admission (OR 1.58; 95%CI, 1.20-2.07) and least likely to receive chest x-rays during hospitalization (OR 0.66; 95%CI, 0.49-0.90); Hispanic children were most likely to be discharged on inhaled corticosteroids (OR 1.92; 95%CI, 1.19-3.10). We observed differences between NHW and minority children regarding pre-admission albuterol use, inpatient diagnostic imaging, and prescription of inhaled corticosteroids at discharge; practices that deviate from the American Academy of Pediatrics guidelines. The causes of these differences require further study but they support implementation of clinical care pathways for severe bronchiolitis.

Development, Implementation, And Evaluation Of An End Of Life Care Online Module For Pre-Clinical Students, Chung Sang Tse

Yale Medicine Thesis Digital Library

Basic palliative and end-of-life care skills are necessary for all physicians regardless of their field of specialty. Education should begin during the early stages of medical training, as early as medical school, to ensure that all physicians acquire a basic understanding of these aspects of medical care. At the Yale School of Medicine, the End-of-Life and Palliative Care Curriculum was formalized in 2008 to address this need and was expanded in 2012 to include an original educational online module. This new module was designed specifically for second-year medical students, who had the opportunity to visit hospice patients but do not …

The Hepatitis C Care Continuum Among Hiv-Infected Patients In A Large Urban Outpatient Clinic: Identifying Barriers To Achieving Optimal Outcomes, Austin Weiss

Yale Medicine Thesis Digital Library

BACKGROUND: Despite the availability of effective therapies for the treatment of chronic hepatitis C virus (HCV) infection, therapeutic benefits have yet to be experienced by patients affected by the disease, including human immunodeficiency virus (HIV) infected individuals. The aims of this study were to describe the continuum of hepatitis C care among HIV/HCV coinfected patients and identify barriers to achieving optimal management outcomes.

DESIGN AND METHODS: We conducted a retrospective analysis of HIV/HCV coinfected patients under care at an urban HIV referral clinic, comprising patients identified with HCV infection from 2002-2014. Electronic medical records of eligible patients were reviewed, capturing …

Stratification Of The Impact Of Inappropriate Empirical Antimicrobial Therapy For Gram-Negative Bloodstream Infections By Predicted Prognosis, Sarah E. Cain, Joseph Kohn, P. Brandon Bookstaver, Helmut Albrecht, Majdi N. Al-Hasan

Faculty Publications

The bloodstream infection mortality risk score (BSIMRS) predicts the outcome of patients with Gram-negative bloodstream infections (BSI) with high discrimination. This retrospective cohort study examined the impact of inappropriate antimicrobial therapy on mortality in adult patients with Gram-negative BSI admitted to Palmetto Health Hospitals in Columbia, SC, USA, from 1 January 2011 to 31 December 2012 after stratification by predicted prognosis at initial presentation using BSIMRS. A multivariate Cox regression model was used to identify independent risk factors for 28-day mortality overall and within each predefined BSIMRS category (<5, 5 to 9, and ≥10). Relative risk reduction (RRR), absolute risk reduction (ARR), and number needed to treat (NNT) were calculated from a predictive logistic regression model of mortality. Overall, 390 unique patients with first episodes of Gram-negative BSI were identified. The median age was 66 years, and 229 (59%) were women. There was significant association between inappropriate antimicrobial therapy and mortality in patients with BSIMRS of 5 to 9 (adjusted hazard ratio [aHR], 3.55; 95% confidence intervals [CI], 1.22 to 8.31; P = 0.02) and BSIMRS of ≥10 (aHR, 4.99; 95% CI, 1.09 to 22.87; P = 0.04) but not in those with BSIMRS of <5 (aHR, 3.34; 95% CI, 0.17 to 22.77; P = 0.34). RRR, ARR, and NNT were 0.25, 0.02, and 63 for BSIMRS of <5; 0.56, 0.32, and 3 for BSIMRS of 5 to 9; and 0.39, 0.39, and 3 for BSIMRS of ≥10, respectively. There is a significant benefit from appropriate antimicrobial therapy in patients with Gram-negative BSI with guarded (BSIMRS of 5 to 9) and poor (BSIMRS of ≥10) predicted prognosis. Survival difference remains unclear among those with good predicted prognosis (BSIMRS of <5) at initial presentation.

Dendritic And Langerhans Cells Respond To Aβ Peptides Differently: Implication For Ad Immunotherapy, Jiang Cheng, Xiaoyang Lin, David Morgan, Marcia Gordon, Xi Chen, Zhen-Hai Wang, Hai-Ning Li, Lan-Jie He, Shu-Feng Zhou, Chuanhai Cao

Molecular Pharmacology & Physiology Faculty Publications

Both wild-type and mutated beta-amyloid (Aβ) peptides can elicit an immune response when delivered subcutaneously. However, only mutated forms of Aβ can sensitize dendritic cells when administered intravenously or intraperitoneally. To understand the role of mutation and delivery routes in creating immune responses, and the function of dendritic cells as therapeutic agents, we used fluorescent-conjugated WT Aβ1-40 (WT40) and artificially mutated Aβ1-40 (22W40) peptides to treat dendritic and Langerhans cells from young and/or old mice at different time points. The cell types were analyzed by flow cytometry and confocal microscopy to identify differences in function and antigen presentation, and Luminex …

Familiarity Breeds Contempt: Combining Proximity Loggers And Gps Reveals Female White-Tailed Deer (Odocoileus Virginianus) Avoiding Close Contact With Neighbors, Marie I. Tosa, Eric M. Schauber, Clayton K. Nielsen

Publications

Social interactions can influence infectious disease dynamics, particularly for directly transmitted pathogens. Therefore, reliable information on contact frequency within and among groups can better inform disease modeling and management. We compared three methods of assessing contact patterns: (1) space-use overlap (volume of interaction [VI]), (2) direct contact rates measured by simultaneous global positioning system (GPS) locations (apart), and (3) direct contact rates measured by proximity loggers (PLs; 1-m detection) among female white-tailed deer (Odocoileus virginianus). We calculated the PL∶GPS contact ratios to see whether both devices reveal similar contact patterns and thus predict similar pathogen transmission patterns. Contact …

Survival And Dispersal Of White-Tailed Deer In The Agricultural Landscape Of East-Central Illinois, Charles W. Anderson, Clayton K. Nielsen, Eric M. Schauber

Publications

White-tailed deer (Odocoileus virginianus) are a keystone species throughout their range in North America. The recent presence of diseases such as chronic wasting disease and bovine tuberculosis in Midwestern North America dictates the examination of influences of those diseases on deer populations, and survival and dispersal rates are important parameters when modeling potential disease spread. We quantified survival and dispersal rates of 105 deer in agriculturally-dominated east-central Illinois during 2005-2009. We used Program MARK to estimate rates of annual survival, seasonal survival, and dispersal for fawn, yearling, and adult age-classes. Male and female seasonal (winter/spring [16 Dec–14 May], summer [15 …

Serotonin 5-Ht2 Receptor Activation Prevents Allergic Asthma In A Mouse Model, Felix Nau, Justin Miller, Jordy Saravia, Terry Ahlert, Bangning Yu, Kyle I. Happel, Stephania A. Cormier, Charles D. Nichols

Faculty Publications

© 2015 the American Physiological Society. Asthma is an inflammatory disease of the lung characterized by airways hyper-responsiveness (AHR), inflammation, and mucus hyperproduction. Current main-stream therapies include bronchodilators that relieve bronchoconstriction and inhaled glucocorticoids to reduce inflammation. The small molecule hormone and neurotransmitter serotonin has long been known to be involved in inflammatory processes; however, its precise role in asthma is unknown. We have previously established that activation of serotonin 5-hydroxytryptamine (5-HT)2A receptors has potent anti-inflammatory activity in primary cultures of vascular tissues and in the whole animal in vasculature and gut tissues. The 5-HT2A receptor agonist, (R)-2,5-dimethoxy-4-iodoamphetamine [(R)-DOI] is …

Mediation And Moderation Analysis Of Nutrition, Inflammatory Biomarkers, And Cognition In Older Adults, Elizabeth Handing

USF Tampa Graduate Theses and Dissertations

Nutrition can be viewed as a modifiable factor related to maintaining and preserving health in older adults. Previous studies have found that nutritional factors can influence cognitive abilities, however few studies have examined macronutrients and micronutrients as they relate to cognitive functioning. Research has yet to examine the mechanisms related to nutrition, cognition and aging in an older adult population from a holistic and interactive perspective.

This dissertation examined three research questions to better understand the relationship between age, nutrition, cognition, and inflammatory biomarkers. First, is nutrition related to cognition beyond demographic factors? Do individual nutrients serve as mediators? Second, …

The Role Of Rnai In Downregulation Of Physcomitrella Patens Defense Genes And The Preliminary Steps Of Peg Mediated Transformation Of Mosses, Adam Beswick

Undergraduate Honors Thesis Collection

When attacked by a pathogen, the moss Physcomitrella patens will undergo both a hypersensitive response (HR) as well as a systematic acquired resistance (SAR) response. The SAR response turns on genes that ready the plant for future pathogen attacks. The hormone jasmonic acid (JA) is one of the products of SAR response in P. patens. In this study interference RNA (RNAi) was used to decrease gene expression of the allene oxide cyclase (ACe) gene, a key gene in the production pathway of JA. Using quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), RNAi was found to significantly lower ACC gene levels …

University Of Texas Southwestern Medical Center V. Nassar: The Supreme Court’S “Heads The Employer Wins, Tails The Employee Loses” Decision, Jenna Hueneger

Saint Louis University Law Journal

No abstract provided.

A Rare Case Of Diffuse Alveolar Hemorrhage Secondary To Acute Pulmonary Histoplasmosis, Kunal Grover, Hadeel Zainah, Shubhita Bhatnagar, Tricia Stein

Infectious Diseases Articles

Diffuse alveolar hemorrhage (DAH) is a rare presentation of acute pulmonary histoplasmosis. While histoplasmosis has been reported to cause hemoptysis and alveolar hemorrhage in children, the English language literature lacks any adult case reports documenting this association. We report a case of pulmonary histoplasmosis where the initial presentation was pneumonia with a subsequent diagnosis of DAH.

The Increasing Weight Of Regulation: Countries Combat The Global Obesity Epidemic, Allyn L. Taylor, Emily Whelan Parento, Laura A. Schmidt

Indiana Law Journal

Obesity is a global epidemic, exacting an enormous human and economic toll. In the absence of a comprehensive global governance strategy, states have increasingly employed a wide array of legal strategies targeting the drivers of obesity. This Article identifies recent global trends in obesity-related legislation and makes the normative case for an updated global governance strategy.

National governments have responded to the epidemic both by strengthening traditional interventions and by developing novel legislative strategies. This response consists of nine important trends: (1) strengthened and tailored tax measures; (2) broadened use of counter-advertising and health campaigns; (3) expanded food labeling; (4) …

Radiation-Induced Esophagitis Is Mitigated By Soy Isoflavones, Matthew Donovan Fountain

Wayne State University Theses

Lung cancer patients receiving radiotherapy present with acute and chronic esophagitis, resulting in pain and difficulties swallowing. These effects are due to radiation injury to the normal tissues in the esophagus. Our previous studies in pre-clinical models of lung cancer and naïve mice have shown that soy isoflavones alleviates radiation-induced toxicity to normal lung, including a decrease in pneumonitis and fibrosis. In this study, we have investigated whether radiation-induced esophagitis can be reduced by soy isoflavones. C57BL/6 mice were treated with 10 Gy or 25 Gy for thoracic irradiation and soy isoflavones given daily at 1mg/mouse up to 16 weeks. …

A Retrospective Cohort Study Of High Prevalence Of St131 Among Extended Spectrum Β-Lactamase Producing E. Coli Among Inpatients In The Metropolitan Detroit Area, Pansy Awasthy

Wayne State University Theses

ABSTRACT

HIGH PREVALENCE OF ST131 AMONG EXTENDED SPECTRUM Β-LACTAMASE PRODUCING E. COLI AMONG INPATIENTS IN THE METROPOLITAN DETROIT AREA

by

PANSY AWSTHY

2015

Advisor: Dr. Emily T. Martin

Major: Pharmaceutical Sciences

Degree: Master of Science

Objectives: E.coli ST131 multi-locus sequence type (MLST) has been associated with extended spectrum β-lactamase (ESBL) production, conferring antimicrobial resistance, with increased virulence and with healthcare-associated infection. The high prevalence of multidrug antimicrobial resistance in ESBL-producing ST131 E.coli infections creates unique challenges in the studying patient outcomes and analysis are required to study ST131 E.coli amongst a large population of ESBL-producers. Our objective was to determine …

A Review Of Factors Associated With The Utilization Of Healthcare Services And Strategies For Improving Postpartum Care In Africa, Danielle Yugbare Belemsaga, Anne Goujon, Joel A. Kiendrebeogo, Els Duysburgh, Seni Kouanda, Olivier Degomme, Marleen Temmerman

Obstetrics and Gynaecology, East Africa

Reducing maternal mortality continues to be a major challenge for African countries. We conducted a literature review to identify the factors associated with the utilization of maternal and child healthcare services during the postpartum period and the strategies for strengthening postpartum healthcare in Africa. We carried out an electronic search in several databases of texts published between 1995 and 2012 related to maternal and child health. Seventy-five publications fitted the eligibility criteria. Our analysis shows that to a large extent the socio-economic context was dominant among the factors associated with the quality and utilization of postpartum services. The best interventions …

Experimental Evolution Of Specialism In A Wild Virus, Alexander Kula

Master's Theses

A pathogen's ability to successfully replicate and persist within a new host population is fraught with obstacles. While an expanded host-range allows for a greater chance at successful replication, such generalists are typically outcompeted by species which have specialized and adapted host-specific features. Although the most ubiquitous species on earth, very few bacteria-infecting viral species (bacteriophages) with truly broad host-ranges have been identified; this is partially due to the fact that only a small fraction of bacteria (and thus likely hosts) are amenable to laboratory conditions. Nevertheless, the processes of expanding as well as narrowing host-range are not well understood …

Metabolic And Metagenomic Outcomes From Early-Life Pulsed Antibiotic Treatment, Sahar Abubucker, Yanjiao Zhou, Makedonka Mitreva, George M. Weinstock, Erica Sodergren, Et Al

Open Access Publications

No abstract provided.

Brown-Pigmented Mycobacterium Mageritense As A Cause Of Prosthetic Valve Endocarditis And Bloodstream Infection, Allison R. Mcmullen, Caline Mattar, Nigar Kirmani, Carey-Ann D. Burnham

Open Access Publications

No abstract provided.

Genome Sequence Of Enterovirus D68 From St. Louis, Missouri, Usa, Kristine M. Wylie, Todd N. Wylie, Anthony Orvedahl, Richard S. Buller, Brandi N. Herter, Vincent Magrini, Richard K. Wilson, Gregory A. Storch

Open Access Publications

No abstract provided.

Wu Polyomavirus In Respiratory Epithelial Cells From Lung Transplant Patient With Job Syndrome, Erica A. Siebrasse, Nang L. Nguyen, Annie Wang, David Wang, Diana V. Pastrana, Mark J. Roth, Steven M. Holland, Alexandra F. Freeman, Christopher B. Buck, John Mcdyer

Open Access Publications

No abstract provided.