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2015

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Articles 51841 - 51870 of 53355

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Endometrial Cytology As A Diagnostic Tool For Subclinical Endometritis In Beef Heifers, Carlos Moscuzza, Guadalupe Alvarez, Betiana Gutierrez, Marcelo Zurita, Marcelo Tropeano, Roberto Perna Jan 2015

Endometrial Cytology As A Diagnostic Tool For Subclinical Endometritis In Beef Heifers, Carlos Moscuzza, Guadalupe Alvarez, Betiana Gutierrez, Marcelo Zurita, Marcelo Tropeano, Roberto Perna

Turkish Journal of Veterinary & Animal Sciences

The obstetric assistance of dystocia in field conditions promotes greater bacterial contamination of the uterus, causing subclinical endometritis. The aim of this study was to determine the occurrence of subclinical endometritis in beef heifers receiving professional calving assistance, and to evaluate endometrial cytology as a diagnostic technique compared to uterine biopsy. A group of 829 Angus heifers were assisted at calving. Dystocia was classified based on difficulty at assistance, which was estimated as complex or simple. At the end of the calving season, 93 dystocic heifers were reported (10.7%). The percentage of simple resolution and complex resolution was 88.2% and …

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Heart Performance Of Lambs And Its Relation To Muscle Volume And Body Surface, Róbert Romvári, Csaba Hancz, Péter Horn, Zsolt Petrási, András Szabó, Dávid Mezöszentgyörgyi, Imre Repa Jan 2015

Heart Performance Of Lambs And Its Relation To Muscle Volume And Body Surface, Róbert Romvári, Csaba Hancz, Péter Horn, Zsolt Petrási, András Szabó, Dávid Mezöszentgyörgyi, Imre Repa

Turkish Journal of Veterinary & Animal Sciences

Electrocardiography-gated dynamic magnetic resonance imaging (MRI) methodology was developed for the in vivo examination of sheep heart characteristics. By combining noninvasive determination of skeletal muscle mass, we studied the relationship between total body skeletal muscle content and heart performance. Measurements were carried out on male Merino lambs using 1.5 T field strength equipment. Average left ventricular volumes were determined and stroke volume was estimated during image postprocessing. Ejection fraction was calculated (73 ± 1.8%) and the cardiac output (CO) value was estimated (2.75 ± 0.16 L/min). After measuring left ventricular wall thickness, contraction values were determined at the septum (62%), …

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Effects Of Alpha Lipoic Acid And Vitamin C Supplementation On Transforming Growth Factor Alpha (Tgf \Alpha) And Transforming Growth Factor Beta 2 (Tgf \Beta2)Secretion In Testicular Tissue Of Diabetic Rats, Sevda Eli̇ş Yildiz, Buket Bakir, Hami̇t Uslu, Ebru Karadağ Sari, Musa Karaman Jan 2015

Effects Of Alpha Lipoic Acid And Vitamin C Supplementation On Transforming Growth Factor Alpha (Tgf \Alpha) And Transforming Growth Factor Beta 2 (Tgf \Beta2)Secretion In Testicular Tissue Of Diabetic Rats, Sevda Eli̇ş Yildiz, Buket Bakir, Hami̇t Uslu, Ebru Karadağ Sari, Musa Karaman

Turkish Journal of Veterinary & Animal Sciences

The effects of alpha lipoic acid and vitamin C supplementation on TGF \alpha and TGF \beta2 secretion in the testicular tissue of streptozotocin-induced diabetic rats were examined by immunohistochemical methods. Rats were divided into 5 groups; group 1 (control), 2 (diabetic control), 3 (diabetes + alpha lipoic acid), 4 (diabetes + vitamin C), and 5 (diabetes + alpha lipoic acid + vitamin C). Hematoxylin and eosin staining was performed in order to examine testicular tissue histopathologically. The streptavidin-biotin-peroxidase complex method was used for histological examination and determination of TGF \alpha and TGF \beta2 localization in the testicular tissue. Regarding fasting …

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Effect Of Freezing Rate On Goat Sperm Morphology And Dna Integrity, Burcu Üstüner, Zekari̇ya Nur, Seli̇m Alçay, Mehmed Berk Toker, Hakan Sağirkaya, Mustafa Kemal Soylu Jan 2015

Effect Of Freezing Rate On Goat Sperm Morphology And Dna Integrity, Burcu Üstüner, Zekari̇ya Nur, Seli̇m Alçay, Mehmed Berk Toker, Hakan Sağirkaya, Mustafa Kemal Soylu

Turkish Journal of Veterinary & Animal Sciences

This study investigates the effect of freezing rates on the spermatological parameters of frozen and thawed Saanen goat semen. Equilibrated semen was frozen at 4 different freezing rates from +5 °C to -150 °C (G10: 10 °C/min, G12: 12 °C/min, G15: 15 °C/min, and G24: 24 °C/min) and stored in liquid nitrogen until use. Semen samples were examined for sperm motility, defective acrosomes (FITC-PSA), and DNA integrity using TUNEL after dilution with extender A at equilibration and postthaw stage. There was no significant difference among the freezing stages in terms of DNA fragmentation (P > 0.05). DNA integrity was partially affected …

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Effect Of Dietary Seaweed (Ulva Lactuca) Supplementation On Growth Performance Of Sheep And On In Vitro Gas Production Kinetics, Ahmed El-Waziry, Ahmed Al-Haidary, Aly Okab, Emad Samara, Khalid Abdoun Jan 2015

Effect Of Dietary Seaweed (Ulva Lactuca) Supplementation On Growth Performance Of Sheep And On In Vitro Gas Production Kinetics, Ahmed El-Waziry, Ahmed Al-Haidary, Aly Okab, Emad Samara, Khalid Abdoun

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to determine the effect of dietary seaweed (Ulva lactuca) supplementation on growth performance of sheep, in vitro gas production, estimated energy, and microbial protein synthesis. A total of 18 Naimey male sheep with average live weight of 22.78 ± 0.24 kg were randomly allocated to 3 groups. Sheep in group 1 were fed a diet containing commercial feed without seaweed as a control diet, sheep in group 2 were fed the control diet with 3% seaweed, and sheep in group 3 were fed the control diet with 5% seaweed. The supplementation of seaweed had no …

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Relationships Between Sarcomere Length And Basic Composition Of Infraspinatus And Longissimus Dorsi Muscle, Dominika Guzek, Dominika Glabska, Krzysztof Glabski, Grzegorz Pogorzelski, Jerzy Barszczewski, Agnieszka Wierzbicka Jan 2015

Relationships Between Sarcomere Length And Basic Composition Of Infraspinatus And Longissimus Dorsi Muscle, Dominika Guzek, Dominika Glabska, Krzysztof Glabski, Grzegorz Pogorzelski, Jerzy Barszczewski, Agnieszka Wierzbicka

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to determine the relationship between sarcomere length and basic composition (water, protein, ash, collagen, and fat content including marbling) of beef muscles with fast-twitch glycolytic (longissimus dorsi muscle) and slow-twitch oxidative fibers (infraspinatus muscle) in Limousin bulls. Samples (n = 15) were obtained from federally inspected slaughter facilities, and animals used in the experiment were treated according to standard ethical norms. The shortest sarcomeres were observed for longissimus dorsi from striploin, and the longest for infraspinatus muscle. Additionally, the influence of both cut and animal was observed. In both cases of muscles, sarcomere length …

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Effects Of Dystocia On Milk Production And Reproduction In Subsequent Lactation In A Turkish Holstein Herd, İbrahi̇m Kaya, Can Uzmay, Tarik Ayyilmaz Jan 2015

Effects Of Dystocia On Milk Production And Reproduction In Subsequent Lactation In A Turkish Holstein Herd, İbrahi̇m Kaya, Can Uzmay, Tarik Ayyilmaz

Turkish Journal of Veterinary & Animal Sciences

The study investigated effects of dystocia on milk production, reproduction, calf loss, and cow culling in a Turkish Holstein herd at the Agricultural Faculty, Ege University, İzmir, Turkey. Data were from 653 single calvings between 2006 and 2010. Considering a greater dystocia incidence in primiparous cows, data was divided in two; 223 primiparous and 430 multiparous calvings scored on a 1-to-5 scale. Scores were categorized into no dystocia and dystocia. With few related observations, all multiparous data were excluded since sample size for multiparous dystocic calvings was too small to make reliable estimates. Milk yields, days to first service, and …

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Association Between The Muc4 G.243a > G Polymorphism And Immune Andproduction Traits In Large White Pigs, Ying Liu, Xue Mei Yin, Ri Wei Xia, Yong Jiu Huo, Guo Qiang Zhu, Sheng Long Wu, Wen Bin Bao Jan 2015

Association Between The Muc4 G.243a > G Polymorphism And Immune Andproduction Traits In Large White Pigs, Ying Liu, Xue Mei Yin, Ri Wei Xia, Yong Jiu Huo, Guo Qiang Zhu, Sheng Long Wu, Wen Bin Bao

Turkish Journal of Veterinary & Animal Sciences

PCR-RFLP analysis of intron 17 of the MUC4 gene was conducted in Large White pigs and associations with cytokine levels (IFN-?, IL-1ß, IL-4, IL-6, IL-8, IL-10, TGF-ß, and TNF-?), production traits (100-kg-weight day age, back-fat depth, and eye muscle depth), and reproductive performance within different litters (total number born, number born alive, number in live weaning litter) were analyzed with the aim of identifying genetic markers of disease resistance. The results revealed AA, AG, and GG genotypes, with an A243G mutation identified in the GG genotype. Correlation analysis indicated that IL-8 and IL-10 levels and 100-kg-weight day age were significantly …

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The Effect Of Oleic And Linoleic Acids On In Vitro Bovineembryonic Development And Embryo Quality, Tahi̇r Karaşahi̇n, Şevket Arikan Jan 2015

The Effect Of Oleic And Linoleic Acids On In Vitro Bovineembryonic Development And Embryo Quality, Tahi̇r Karaşahi̇n, Şevket Arikan

Turkish Journal of Veterinary & Animal Sciences

The present study was aimed at the investigation of the effects of oleic and linoleic acid on the in vitro development of Anatolian native black crossbred bovine embryos. Following the in vitro maturation and fertilization of oocytes, embryonic development stages were monitored using embryo culture medium supplemented with three different doses of oleic and linoleic acid (10, 100, and 1000 µM). Examination of the culture media 48 h after fertilization demonstrated that linoleic acid had no effect on embryo cleavage rates, while oleic acid produced a significant increase in cleavage rates (P < 0.01). It was determined that the highest dose of linoleic acid (1000 µM) and all three doses of oleic acid significantly increased the proportion of cultured oocytes developing to the morula-blastocyst stage (P < 0.001). As a result, while oleic acid significantly increased (P < 0.001) the proportion of embryos developing to the morula-blastocyst stage, linoleic acid had limited effects on embryonic development and quality. Thus, it was concluded that the supplementation of Anatolian native black crossbred bovine embryo cultures with oleic acid may induce positive effects on embryonic development and quality.

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In Vivo Assessment Of Dna Damage In Cyprinus Carpio After Exposure To Potassium Dichromate Using Rapd, Pavan Kumar, Ravindra Kumar, Naresh Sahebrao Nagpure, Prakash Nautiyal, Basdeo Kushwaha, Christopher Didigwu Nwani, Wazir Singh Lakra Jan 2015

In Vivo Assessment Of Dna Damage In Cyprinus Carpio After Exposure To Potassium Dichromate Using Rapd, Pavan Kumar, Ravindra Kumar, Naresh Sahebrao Nagpure, Prakash Nautiyal, Basdeo Kushwaha, Christopher Didigwu Nwani, Wazir Singh Lakra

Turkish Journal of Veterinary & Animal Sciences

The present study used the random amplification of polymorphism DNA (RAPD) technique to evaluate possible DNA damage in the blood cells of Cyprinus carpio exposed to potassium dichromate. Test specimens were exposed to 3 sublethal test concentrations (93.95, 187.9, and 281.85 mg L^{?1}) of potassium dichromate for a period of 96 h. The samplings were collected at 24, 48, 72, and 96 h after exposure. DNA was isolated from the blood samples of both the control and treated groups of C. carpio, and 3 selected decamer primers were used for RAPD-PCR. In comparison to the control, potassium dichromate-treated groups showed …

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In Vitro Gas Production Of Five Rations Of Different Maize Silage And Concentrate Ratios Influenced By Increasing Levels Of Chemically Characterized Extract Of Salix Babylonica, Mona Mohamed Mohamed Yasseen Elghandour, Ahmed Eid Kholif, Adrian Zaragoza Bastida, Dora Luz Pinzón Martínez, Abdelfattah Zeidan Mohamed Salem Jan 2015

In Vitro Gas Production Of Five Rations Of Different Maize Silage And Concentrate Ratios Influenced By Increasing Levels Of Chemically Characterized Extract Of Salix Babylonica, Mona Mohamed Mohamed Yasseen Elghandour, Ahmed Eid Kholif, Adrian Zaragoza Bastida, Dora Luz Pinzón Martínez, Abdelfattah Zeidan Mohamed Salem

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to assess the effect of the chemical substances of Salix babylonica (SB) extract on in vitro rumen fermentation of five mixed rations with different maize silage to concentrate ratios. Fifty-nine compounds were identified in SB extract using the retention time and mass spectral technique. Interactive effects were noted (P < 0.001) for the asymptotic gas production (GP) (b), the rate of production (c), the initial delay before GP begins (L), pH, dry matter digestibility, metabolizable energy (ME), organic matter digestibility (OMD), short chain fatty acids (SCFAs), gas yield at 24 h (GY_{24}), microbial crude protein, and in vitro GP. Both 1.2 and 1.8 mL SB/g DM had the highest (P < 0.05) b and c values. Addition of 1.2 and 1.8 mL SB/g DM linearly improved (P < 0.001) ME, OMD, SCFAs, and GY24. It could be concluded that, based on the highly detected interaction effects between ration types and extract doses for fermentation parameters and GP, the most effective dose of SB varied between incubated total mixed rations. However, the ration of 25% silage and 75% concentrate had the highest nutritive value, especially at doses of 1.2 to 1.8 mL SB/g DM total mixed ration.

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Effects Of Dietary Supplementation Of Probiotics (Bacillus Subtilis, Bacillus Licheniformis, And Bacillus Natto) On Broiler Muscle Development And Meat Quality, Xianjian Zhou, Erhui Jin, Shenghe Li, Chenfang Wang, Enmei Qiao, Guozhong Wu Jan 2015

Effects Of Dietary Supplementation Of Probiotics (Bacillus Subtilis, Bacillus Licheniformis, And Bacillus Natto) On Broiler Muscle Development And Meat Quality, Xianjian Zhou, Erhui Jin, Shenghe Li, Chenfang Wang, Enmei Qiao, Guozhong Wu

Turkish Journal of Veterinary & Animal Sciences

This paper aims to evaluate the effects of probiotics (Bacillus subtilis, Bacillus licheniformis, and Bacillus natto) on muscle development and meat quality of broilers. In this study, 480 broilers (1 day old) were randomly divided into 4 groups. Group A was fed with a basal diet. The basal diets of Group B, Group C, and Group D were respectively supplemented with 50 mg/kg chlortetracycline, 200 mg/kg probiotics, and 400 mg/kg probiotics. On day 21 and day 42, in each group, 18 randomly selected broilers were tested to determine muscle performances. On day 21, compared with Group A, Group C and …

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Temperature Range Analysis (T_{Max}) On Dorsal Surface Of Sporting Horses, Ewa Jodkowska, Krzysztof Dudek, Maria Soroko Jan 2015

Temperature Range Analysis (T_{Max}) On Dorsal Surface Of Sporting Horses, Ewa Jodkowska, Krzysztof Dudek, Maria Soroko

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine temperature differences on the dorsal surface of 32 clinically healthy show jumping horses. Temperature was measured using a Thermovision 550 (FLIR) camera, determining maximum temperature (T_{max}). The horse?s body was divided into the following regions: neck, withers, cranial back, caudal back, loins, and hindquarters. Those regions were subdivided into middle, left, and right parts. Statistical methods used for calculations consisted of Wilcoxon?s signed rank test and Dunn?s post hoc comparison test. Temperatures on the dorsal surface ranged from 19.5 to 31.5 °C, with the lowest indicated in the withers area and the …

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Serological And Molecular Diagnosis Of Paratuberculosis In Dairy Cattle, Kadi̇r Semi̇h Gümüşsoy, Tuba İça, Seçi̇l Abay, Fuat Aydin, Harun Hizlisoy Jan 2015

Serological And Molecular Diagnosis Of Paratuberculosis In Dairy Cattle, Kadi̇r Semi̇h Gümüşsoy, Tuba İça, Seçi̇l Abay, Fuat Aydin, Harun Hizlisoy

Turkish Journal of Veterinary & Animal Sciences

In this study, the presence of paratuberculosis was investigated by serological and molecular methods in herds of dairy cattle. Blood, milk, and stool samples of 147 cows aged 2 years old or older with chronic diarrhea were collected. A California mastitis test (CMT) was performed on milk samples. Indirect paratuberculosis enzyme-linked immunosorbent assay (ELISA) test was used for serological investigation. Polymerase chain reaction (PCR) was utilized for molecular identification of Mycobacterium avium subsp. paratuberculosis (MAP) from milk and stool samples. Immunomagnetic separation (IMS) method was used for milk and fecal samples. Eighteen (12.24%), 44 (29.93%), 36 (24.49%), 28 (19.05%), and …

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Immunohistochemical Distribution Of Platelet-Derived Growth Factor-C And Platelet-Derived Growth Factor Receptor-? In Small Intestine Of Rats Treated With Capsaicin, Buket Bakir, Ebru Karadağ Sari Jan 2015

Immunohistochemical Distribution Of Platelet-Derived Growth Factor-C And Platelet-Derived Growth Factor Receptor-? In Small Intestine Of Rats Treated With Capsaicin, Buket Bakir, Ebru Karadağ Sari

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to examine the effect of capsaicin administration immunohistochemically on platelet-derived growth factor (PDGF)-C and platelet-derived growth factor receptor (PDGFR)-\alpha release in the small intestine tissues of rats. Rats were divided into three groups: group 1 (subcutaneous drug administration), group 2 (oral drug administration), and group 3 (subcutaneous sham administration). Histometric measurements and counts were performed in order to determine whether there was a difference between groups in terms of height of villi, depth of crypts, and goblet cell count in small intestine. The small intestine was examined by Crossman?s triple staining method and streptavidin-biotin …

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Influences Of Flunixin And Tenoxicam On The Pharmacokinetics Of Florfenicol In Lipopolysaccharide-Induced Endotoxemia, Feri̇de Koç, Alptuğ Ati̇la, Emre Karakuş, Kami̇l Üney Jan 2015

Influences Of Flunixin And Tenoxicam On The Pharmacokinetics Of Florfenicol In Lipopolysaccharide-Induced Endotoxemia, Feri̇de Koç, Alptuğ Ati̇la, Emre Karakuş, Kami̇l Üney

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to investigate the influences of flunixin (FM) and tenoxicam (TN) on the pharmacokinetics of florfenicol (FF) after coadministration in lipopolysaccharide (LPS)-induced endotoxemic rabbits. Fifteen male rabbits were used in this study. FF (20 mg/kg), FM (2 mg/kg), and TN (1 mg/kg) were coadministered via intravenous injection to the animals. The concentrations of FF were determined by high-performance liquid chromatography with diode-array detection from 0.08 to 12 h in plasma. The plasma concentration-time profile of FF was described using a noncompartmental open model. In this study, terminal half-life, area under the curve, mean residence time, …

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The Effects Of Hypoxia On The Expression Of Vascular Endothelialgrowth Factor In Broiler Lung Fibroblasts, Musa Özgür Özyi̇ği̇t, Müjdat Müfi̇t Kahraman, Ahmet Akkoç Jan 2015

The Effects Of Hypoxia On The Expression Of Vascular Endothelialgrowth Factor In Broiler Lung Fibroblasts, Musa Özgür Özyi̇ği̇t, Müjdat Müfi̇t Kahraman, Ahmet Akkoç

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate the effects of hypoxic stresses on vascular endothelial growth factor (VEGF) in cultured lung fibroblasts of meat-type chickens. Isolated and subcultured primary lung fibroblasts were exposed to hypoxia. The presence of VEGF and the effects of hypoxia on cultured lung fibroblasts and in the culture media were evaluated. Immunocytochemistry using streptavidin-biotin-peroxidase method and ELISA using commercially available primary monoclonal antibodies were used for evaluation in both 6-h and 12-h hypoxic fibroblasts. The level of VEGF was slightly higher in the 6-h hypoxic group (1.22 ng/mL) than in the 6-h control group (1.27 …

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Influence Of Dietary Zilpaterol Hydrochloride On Feedlot Performance, Carcass Traits, Chemical Composition Of Longissimus Muscle, And Plasma Metabolites Of Castrated Male Goats, Ali Hatefi, Armin Towhidi, Abolfazl Zali, Saeed Zeinoaldini, Mahdi Ganjkhanlou, Reza Masoudi, Alejandro Plascencia Jan 2015

Influence Of Dietary Zilpaterol Hydrochloride On Feedlot Performance, Carcass Traits, Chemical Composition Of Longissimus Muscle, And Plasma Metabolites Of Castrated Male Goats, Ali Hatefi, Armin Towhidi, Abolfazl Zali, Saeed Zeinoaldini, Mahdi Ganjkhanlou, Reza Masoudi, Alejandro Plascencia

Turkish Journal of Veterinary & Animal Sciences

To evaluate the influence of zilpaterol hydrochloride (ZH) supplementation on growth performance, carcass traits, chemical characteristics of the longissimus muscle (LM), and plasma metabolites, 16 Mahabadi castrated goats (29.93 ± 1.84 kg) were individually fed a finishing diet without or with ZH supplementation dosed at 0.20 mg/kg BW daily. Zilpaterol was fed for 30 days with 3 days of withdrawal before harvest. Compared with the controls, ZH supplementation decreased dry matter intake and increased total weight gain, average daily gain, and gain for feed. Hot carcass weight, dressing percentage, and LM area were increased, but back fat thickness and kidney-pelvic …

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Identification Of Simple Sequence Repeat Markers In The Dromedary(Camelus Dromedarius) Genome By Next-Generation Sequencing, Monther Sadder, Hussein Migdadi, Ahmed Al-Haidary, Aly Okab Jan 2015

Identification Of Simple Sequence Repeat Markers In The Dromedary(Camelus Dromedarius) Genome By Next-Generation Sequencing, Monther Sadder, Hussein Migdadi, Ahmed Al-Haidary, Aly Okab

Turkish Journal of Veterinary & Animal Sciences

The availability of molecular markers in camels is limited. The aim of this study was to develop new simple sequence repeat (SSR) markers. Four breeds of pooled dromedary genome were sequenced at low coverage utilizing Roche and Illumina platforms. A total of 65,746 contigs, covering approximately 52 Mb (2316 contigs > 2 kb), were assembled. The partial genome revealed 613 SSR loci with a minimum number of 5 repeat units. Comparative chromosomal location for 60 camel loci was predicted against bovine genome assembly Baylor Btau_4.6.1/bosTau7. Ten markers (16.7%) returned matches with a >100 score and >80% identity. SSR abundance was 1 …

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Concentrations Of Manganese, Iron, And Strontium In Bones Of Thedomestic Dog (Canis Lupus Familiaris), Halina Budis, Elzbieta Kalisinska, Natalia Lanocha, Danuta Kosik_Bogacka Jan 2015

Concentrations Of Manganese, Iron, And Strontium In Bones Of Thedomestic Dog (Canis Lupus Familiaris), Halina Budis, Elzbieta Kalisinska, Natalia Lanocha, Danuta Kosik_Bogacka

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine concentrations of manganese (Mn), iron (Fe), and strontium (Sr) in the cartilage, compact bone, spongy bone, and cartilage with adjacent compact bone of the domestic dog (Canis lupus familiaris). The experiment was carried out on bones from the hip joints of 24 dogs (dogs of 8 years) from northwestern Poland. The concentrations of Mn, Fe, and Sr were assessed by atomic absorption spectrophotometry. Fe concentration was the highest in the cartilage and compact bone. Mn concentration was the lowest in all three types of bone material and had a significantly lower concentration …

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The Assembly Of Virus-Like Particles Of Porcine Transmissible Gastroenteritisvirus In Vitro By Baculovirus Expression System, Song Zhenhui, Haibo Feng, Zhi Zhu, Xianjin Dai, Yue Zhou, Yuntian Li, Xinzhi Cao Jan 2015

The Assembly Of Virus-Like Particles Of Porcine Transmissible Gastroenteritisvirus In Vitro By Baculovirus Expression System, Song Zhenhui, Haibo Feng, Zhi Zhu, Xianjin Dai, Yue Zhou, Yuntian Li, Xinzhi Cao

Turkish Journal of Veterinary & Animal Sciences

Virus-like particles (VLPs) produced by recombinant expression of the major viral structural proteins could be an attractive method for transmissible gastroenteritis virus (TGEV) assembly and release. In this study, the M gene, sM gene, and N gene of TGEV were inserted into donor plasmid pFastBac Dual. Those recombinant donor plasmids were transformed into E.coli DH10Bac respectively. Recombinant baculoviruses were obtained by transfection of the Sf9 cells with Bacmid-M, Bacmid-sM, and Bacmid-N. The results of indirect immunofluorescence assay showed that recombinant M protein, sM protein, and N protein were expressed successfully. In vitro experiments of VLP assembly of TGEV were performed …

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The Relationships Between Brucella Melitensis Predilection Sites, Bacterial Loads In Vivo, And The Agglutinating Antibody Response In Experimentally Infected Sheep, Xiaolei Gao, Yu Kuang, Lintao Ma, Yanli Lu, Qingmin Wu Jan 2015

The Relationships Between Brucella Melitensis Predilection Sites, Bacterial Loads In Vivo, And The Agglutinating Antibody Response In Experimentally Infected Sheep, Xiaolei Gao, Yu Kuang, Lintao Ma, Yanli Lu, Qingmin Wu

Turkish Journal of Veterinary & Animal Sciences

F?or exploring Brucella melitensis survival in in vivo predilection sites and the relationship between bacterial loads and the detection of antibody titers in experimentally subcutaneously infected sheep with B.1397645907melitensis 16M, ten rams and ten ewes were used. One ram and one ewe were euthanized at 7, 15, 30, 60, 90, 120, and 180 days post inoculation (dpi). Bacteriological results showed that tissue isolation rates and bacterial loads peaked from 7 dpi to 30 dpi and then cleared until 120 dpi. In in situ hybridization trials the strongest signals for B.1397645907melitensis were detected at 7 dpi and 15 dpi, and then …

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Comparing Levels Of Certain Heavy Metals And Minerals And Antioxidativemetabolism In Cows Raised Near And Away From Highways*, Hüsametti̇n Eki̇ci̇, Özkan Şi̇mşek, Şevket Arikan, Meryem Eren, Bayram Güner Jan 2015

Comparing Levels Of Certain Heavy Metals And Minerals And Antioxidativemetabolism In Cows Raised Near And Away From Highways*, Hüsametti̇n Eki̇ci̇, Özkan Şi̇mşek, Şevket Arikan, Meryem Eren, Bayram Güner

Turkish Journal of Veterinary & Animal Sciences

The scope of this study was to investigate the concentrations of certain heavy metals, mineral levels, and antioxidative metabolism in cows raised near (300 m) and away from highways (2.5 km) in Çankırı Province. For this purpose, blood samples were collected from 100 cows (aged 3-5 years) raised near and away from highways in this province. Concentrations of certain heavy metals and minerals in the serum were analyzed on an ICP-MS device. Additionally, lipid peroxidation product malondialdehyde (MDA) in the plasma and antioxidative defense system indicators, erythrocyte superoxide dismutase (SOD) and catalase (CAT) activities, were measured with a spectrophotometer. As …

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Impact Of B. Melitensis Rev-1 Vaccination On Brucellosis Prevalence, Adosinda Maria Coelho, Maria De Lurdes Pinto, Juan Garcia Diez, Ana Claudia Coelho Jan 2015

Impact Of B. Melitensis Rev-1 Vaccination On Brucellosis Prevalence, Adosinda Maria Coelho, Maria De Lurdes Pinto, Juan Garcia Diez, Ana Claudia Coelho

Turkish Journal of Veterinary & Animal Sciences

Trás-os-Montes e Alto Douro, in the northeastern region of Portugal, previously exhibited the highest brucellosis prevalence in the entire small ruminant population of Portugal. Consequently, a vaccination program of the whole population with Brucella melitensis Rev-1 was carried out from 2001 to 2004, and further compulsory Rev-1 vaccination of 3- to 6-month-old lambs and kids was carried out between 2005 and 2007. The prevalence of brucellosis decreased by 71.42% in 2004, with a 2-fold decrease occurring from 2005 to 2007. The reduction of brucellosis prevalence was statistically significant (P < 0.001) according to flock size. By species, brucellosis prevalence decreased 14.5-fold in sheep and 5.5-fold in goats in 2004. Regarding flock constitution, it decreased by 77% for pure flocks and 73% in mixed flocks in 2004. Regarding the animals involved in production, brucellosis prevalence decreased during the 7-year period. It was concluded that Rev-1 vaccination of the whole population was effective in decreasing brucellosis prevalence. These results contribute to the scarce information available regarding the effect of Rev-1 vaccination on different characteristics of flocks. They may be used to improve the efficiency of brucellosis eradication programs within livestock management.

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Y Chromosome Haplotype Characterization Of Tunisian Sheep Breeds, Samia Kdidi, Mohamed Habib Yahyaoui, Belen García-Manrique, Pilar Sarto, Mohsen Ben Sassi, Touhami Khorchani, Jorge Hugo Calvo Jan 2015

Y Chromosome Haplotype Characterization Of Tunisian Sheep Breeds, Samia Kdidi, Mohamed Habib Yahyaoui, Belen García-Manrique, Pilar Sarto, Mohsen Ben Sassi, Touhami Khorchani, Jorge Hugo Calvo

Turkish Journal of Veterinary & Animal Sciences

This study aimed to investigate Y chromosomal haplotypes in the main Tunisian sheep breeds. For this purpose, we sequenced 2 regions of SRY (549-bp and 598-bp fragments) and 1 region in each of the AMELY and DBY genes in Barbarin, Western Thin Tail, Sicilo Sarde, and Black Thibar breeds. In addition, we analyzed the diversity at the SRYM18 microsatellite locus and detected 4 alleles of 139, 141, 143, and 145 bp. The sequencing analysis did not reveal any polymorphism in the AMELY or DBY regions; however, 2 samples carried the G allele in the 549-bp fragment of SRY. A total …

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Estimating Allele Frequencies Of Some Hereditary Diseases In Holstein Cattlereared In Burdur Province, Turkey, Özgecan Korkmaz Ağaoğlu, Ali̇ Reha Ağaoğlu, Mustafa Saatci Jan 2015

Estimating Allele Frequencies Of Some Hereditary Diseases In Holstein Cattlereared In Burdur Province, Turkey, Özgecan Korkmaz Ağaoğlu, Ali̇ Reha Ağaoğlu, Mustafa Saatci

Turkish Journal of Veterinary & Animal Sciences

The economics of dairy cattle breeding around the world has been significantly affected by the autosomal recessive hereditary diseases of bovine leukocyte adhesion deficiency (BLAD), factor XI deficiency (FXID), bovine citrullinemia (BC), and deficiency of uridine monophosphate synthase (DUMPS). The aim of this study was to determine BLAD, FXID, BC, and DUMPS alleles in Holstein cattle raised in Burdur Province in Turkey. This study involved taking blood samples randomly from 500 head of Holstein cattle at various dairy farms in different towns in Burdur Province. Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis was employed to identify cows …

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The Effect Of Different Body Weight Categories On Plasma Macromineral Levels In Four Close-Bred Flocks Of Adult Japanese Quails (Coturnix Coturnix Japonica), Ahmed Sultan Jatoi, Abdul Waheed Sahota, Muhammad Akram, Khalid Javed, Muhammad Hayat Jaspal, Jibran Hussain, Shahid Mehmood, Yassar Abbas, Amjad Hussain Mirani, Hafiz Muhammad Ishaq Jan 2015

The Effect Of Different Body Weight Categories On Plasma Macromineral Levels In Four Close-Bred Flocks Of Adult Japanese Quails (Coturnix Coturnix Japonica), Ahmed Sultan Jatoi, Abdul Waheed Sahota, Muhammad Akram, Khalid Javed, Muhammad Hayat Jaspal, Jibran Hussain, Shahid Mehmood, Yassar Abbas, Amjad Hussain Mirani, Hafiz Muhammad Ishaq

Turkish Journal of Veterinary & Animal Sciences

The present study was conducted to investigate plasma macrominerals (Ca, P, Na, K, and Mg) in adult male and female quails according to different body weights in four close-bred flocks of Japanese quails (Coturnix coturnix japonica) maintained at the Avian Research and Training Center of the University of Veterinary and Animal Sciences, Lahore. The results showed that mean plasma Na concentration was significantly (P < 0.05) different in female quails with respect to body size categories. The interaction between flocks and body size for plasma Ca levels was significant (P < 0.05) in both sexes of quails, whereas plasma Na was significant (P < 0.05) in female quails. The mean plasma P and K levels in imported and local flocks of Japanese quails were significantly (P < 0.05) different in female quails, whereas plasma Mg was significantly (P < 0.05) different in male quails. However, with respect to body size categories, plasma P, K, and Mg were significantly (P < 0.05) different in female quails only. The interaction between flocks and body size was significant for K and P in female quails and for plasma Mg levels in both sexes of quails.

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Effects Of Fermented Cottonseed Meal On The Growth Performance, Apparent Digestibility, Carcass Traits, And Meat Composition In Yellow-Feathered Broilers, Cunxi Nie, Wenju Zhang, Wenxia Ge, Yongqiang Wang, Yangfeng Liu, Jiancheng Liu Jan 2015

Effects Of Fermented Cottonseed Meal On The Growth Performance, Apparent Digestibility, Carcass Traits, And Meat Composition In Yellow-Feathered Broilers, Cunxi Nie, Wenju Zhang, Wenxia Ge, Yongqiang Wang, Yangfeng Liu, Jiancheng Liu

Turkish Journal of Veterinary & Animal Sciences

The effects of supplementing broilers' diets with fermented cottonseed meal (FCSM) on the broilers' performance, apparent digestibility, carcass traits, and meat composition were studied. A total of 180 yellow-feathered chickens of 21 days old were randomly divided into three groups (six replicates of 10 broilers each): 1) a control group (Con), 2) a treatment group of FCSM by Candida tropicalis (Ct), and 3) a treatment group of FCSM by C. Tropicalis plus Saccharomyces cerevisiae (Ct-Sc). Results showed that FCSM supplementation improved the average daily gain and gain-to-feed ratio from the 43rd to 64th and the 21st to 64th day, respectively …

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Relationships Of Fatty Acid Group Contents In Milk And Reproductiveperformance In Holstein Cows, Jaromír Duchácek, Jan Beran, Martin Ptácek, Ludek Stádník, Monika Okrouhla, Renata Tousová, Martina Dolezalova Jan 2015

Relationships Of Fatty Acid Group Contents In Milk And Reproductiveperformance In Holstein Cows, Jaromír Duchácek, Jan Beran, Martin Ptácek, Ludek Stádník, Monika Okrouhla, Renata Tousová, Martina Dolezalova

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to evaluate the relationships between milk fatty acid group contents and subsequent reproductive performance in Holstein cows. A total of 27 cows in the 1st to 4th lactations were included in the evaluation. Cows were divided into primiparous and multiparous groups. Daily milk yields increased in the first 8 weeks of lactation. Body condition score decreased until the third month of lactation. As the cows gradually recovered from the negative energy balance, the contents of saturated fatty acids (SFAs) increased, whereas the contents of monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs) decreased. …

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Changes In Arginase Activity And Ast Enzyme Levels In The Cardiac Andskeletal Muscle And Liver Of Lambs With White Muscle Disease, Başak Hanedan, Mi̇ne Eri̇şi̇r, Fati̇h Mehmet Kandemi̇r, Mustafa Özkaraca Jan 2015

Changes In Arginase Activity And Ast Enzyme Levels In The Cardiac Andskeletal Muscle And Liver Of Lambs With White Muscle Disease, Başak Hanedan, Mi̇ne Eri̇şi̇r, Fati̇h Mehmet Kandemi̇r, Mustafa Özkaraca

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to evaluate arginase activity and aspartate aminotransferase (AST) levels in the tissues of the cardiac and skeletal muscle and liver of lambs with white muscle disease (WMD). The cardiac and skeletal muscle and liver tissues were obtained from 8 lambs with WMD and 9 apparently healthy lambs. The diagnosis of WMD was made with the detection of hyaline degeneration upon histopathological examination. A significant increase in arginase activity in the cardiac and skeletal muscle and liver tissues and a significant decrease in AST levels in the cardiac muscle were detected in the WMD group …

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