Digital Commons Network^™

Expression And Misexpression Of The Mir-183 Family In The Developing Hearing Organ Of The Chicken, Kaidi D. Zhang, Michelle L. Stoller, Donna M. Fekete

Department of Biological Sciences Faculty Publications

The miR-183 family consists of 3 related microRNAs (miR-183, miR-96, miR-182) that are required to complete maturation of primary sensory cells in the mammalian inner ear. Because the level of these microRNAs is not uniform across hair cell subtypes in the murine cochlea, the question arises as to whether hair cell phenotypes are influenced by microRNA expression levels. To address this, we used the chicken embryo to study expression and misexpression of this gene family. By in situ hybridization, expression of all 3 microRNAs is robust in immature hair cells of both auditory and vestibular organs and is present in …

Intersection Of Art And Science, Petronio Bendito, Tim Korb

Lawson Building Exhibitions on the Intersection of Art and Science

The Intersection of Art and Science exhibition is an interdisciplinary educational project that examines a wide range of expressive approaches explored by international artists working at the intersection of art, mathematics, computer science, and technology. It is a joint collaboration between the Department of Computer Science and the Patti and Rusty Rueff School of Visual and Performing Arts at Purdue University. Featured artists: Sergio Albiac, Anne Burns, Conan Chadbourne, Hans Dehlinger, Brian Evans, Richard Hassell, Patrick Bingham-Hall, John Arden Hiigli, So Yoon Lym, Gabriel Meyer, and Robert M. Spann. The exhibition was curated by Dr. Petronio Bendito and Dr. Tim …

Metatranscriptomic Profiles Of Eastern Subterranean Termites, Reticulitermes Flavipes (Kollar) Fed On Second Generation Feedstocks, Swapna Priya Rajarapu, Jacob T. Shreve, Ketaki P. Bhide, Jyothi Thimmapuram, Michael E. Scharf

Department of Entomology Faculty Publications

Background: Second generation lignocellulosic feedstocks are being considered as an alternative to first generation biofuels that are derived from grain starches and sugars. However, the current pre-treatment methods for second generation biofuel production are inefficient and expensive due to the recalcitrant nature of lignocellulose. In this study, we used the lower termite Reticulitermes flavipes (Kollar), as a model to identify potential pretreatment genes/enzymes specifically adapted for use against agricultural feedstocks. Results: Metatranscriptomic profiling was performed on worker termite guts after feeding on corn stover (CS), soybean residue (SR), or 98% pure cellulose (paper) to identify (i) microbial community, (ii) pathway …

Taxation On Morals, Alex J. Fulop

Williams Honors College, Honors Research Projects

This paper discusses five (5) tax topics and their relationship to the government attempting to influence taxpayer behavior. These topics include the charitable contribution deduction, excise taxes, §179 deductions, the first-time homebuyer credit, and soil, water, and endangered species conservation expense deductions. The above aspects of tax law are the government’s way of encouraging some behaviors and discouraging others. I believe that doing so is well within the government’s rights to do. However, I also think that encouraging moral actions is different from requiring them, and they certainly should not be required.

The Psychological Benefits Of Animal Assisted Therapy On Elderly Nursing Home Patients, Sophia M. Elliott

Williams Honors College, Honors Research Projects

This literature review of the benefits of Animal Assisted Therapy (AAT) is centered on four scholarly articles published in major psychological journals discussing the reduction of loneliness, anxiety, depression, and cognitive impairments as well as the increase in well-being and happiness in the elderly nursing- home population. Many time these residents are abandoned by family and experience little- to- no interaction with others due to understaffing at these homes. My goal through this literature review was to bring attention to an alternative form of therapy for these patients. Often patients grow sick from depression and lose their sense of self-worth …

Utilizing Ultrasonic Technology To Manage Algal Blooms In Lake Rockwell, Mackenzie Kaser, Andrew Perdue

Williams Honors College, Honors Research Projects

The purpose of this paper is to analyze the effectiveness of ultrasonic technology to manage algal blooms in Lake Rockwell. Lake Rockwell is the drinking water source for the city of Akron, OH and the surrounding areas. One of the main issues facing the City of Akron Water Treatment Plant is cyanobacteria, or blue-green algae. Cyanobacteria releases toxins, which makes conventional water treatment processes more difficult. Our proposed solution to manage algal blooms in Lake Rockwell is ultrasonic technology. This system emits vibrations that destroy the algal cells and prevent new blooms from forming. Our plan involves three master buoys …

Led Learning Guitar, Kellen C. Reusser, Justin Fiser

Williams Honors College, Honors Research Projects

The goal of this project is to create an electric guitar that detects and displays chords in different tunings for learning and exploration purposes. A hexaphonic pickup will generate the required signals and a processor will detect the pitch. The computer software will apply the pitch information to determine the tuning and the voicings of chords. LEDs mounted on the fretboard of the guitar will indicate a note to be played.

In Game Engine Documentation, Zachary A. Pierce

Williams Honors College, Honors Research Projects

The In Game Engine is a game that simulates the functionality of a game engine through simple tasks such as entity creation, interaction, management, and behavior through a user interface. The project was created in the Unreal Engine and utilizes the Blueprints Visual Scripting system to handle these tasks for developers.

What Do Experienced Water Managers Think Of Water Resources Of Our Nation And Its Management Infrastructure?, Faisal Hossain, Jeffrey R. Arnold, Edward Beighley, Casey M. Brown, Steven J. Burian, Ji Chen, Anindita Mitra, Dev Dutta S Niyogi, Roger A. Pielke, Vincent C. Tidwell, Dave Wegner

Department of Earth, Atmospheric, and Planetary Sciences Faculty Publications

This article represents the second report by an ASCE Task Committee "Infrastructure Impacts of Landscape-driven Weather Change" under the ASCE Watershed Management Technical Committee and the ASCE Hydroclimate Technical Committee. Herein, the 'infrastructure impacts" are referred to as infrastructure-sensitive changes in weather and climate patterns (extremes and non-extremes) that are modulated, among other factors, by changes in landscape, land use and land cover change. In this first report, the article argued for explicitly considering the well-established feedbacks triggered by infrastructure systems to the land-atmosphere system via landscape change. In this report by the ASCE Task Committee (TC), we present the …

The Ordinal Effects Of Ostracism: A Meta-Analysis Of 120 Cyberball Studies, Chris Hubertus Joseph Hartgerink, Ilja Van Beest, Jelte M. Wicherts, Kipling D. Williams

Department of Psychological Sciences Faculty Publications

We examined 120 Cyberball studies (N=11,869) to determine the effect size of ostracism and conditions under which the effect may be reversed, eliminated, or small. Our analyses showed that (1) the average ostracism effect is large (d > |1.4|) and (2) generalizes across structural aspects (number of players, ostracism duration, number of tosses, type of needs scale), sampling aspects (gender, age, country), and types of dependent measure (interpersonal, intrapersonal, fundamental needs). Further, we test Williams's (2009) proposition that the immediate impact of ostracism is resistant to moderation, but that moderation is more likely to be observed in delayed measures. Our findings …

Why Social Pain Can Live On: Different Neural Mechanisms Are Associated With Reliving Social And Physical Pain, Meghan L. Meyer, Kipling D. Williams, Naomi I. Eisenberger

Department of Psychological Sciences Faculty Publications

Although social and physical pain recruit overlapping neural activity in regions associated with the affective component of pain, the two pains can diverge in their phenomenology. Most notably, feelings of social pain can be re-experienced or "relived," even when the painful episode has long passed, whereas feelings of physical pain cannot be easily relived once the painful episode subsides. Here, we observed that reliving social (vs. physical) pain led to greater self-reported re-experienced pain and greater activity in affective pain regions (dorsal anterior cingulate cortex and anterior insula). Moreover, the degree of relived pain correlated positively with affective pain system …

Applying Problem-Of-Practice Methods From The Discipline Of Higher Education Within The Justice System: Turning The Concept Of Therapy Dogs For Child Victims Into A Statewide Initiative., Jessie Holton

Electronic Theses and Dissertations

This Dissertation-in-Practice introduces a law enforcement concept-to-practice model designed by combining tested methods of organizational analysis often utilized by those in the discipline of education. The model incorporates a two phase design with the first phase focusing on implementing and evaluating innovative changes within a medium size law enforcement agency for a micro-level analysis. A second phase examines the ability to replicate the concept program on a statewide, macro-level, by incorporating a re-design method utilizing organizational resource and structure frames. The concept applied to this model was the introduction of a therapy dog interaction during investigations involving crimes against children …

Motor Imagery Classification Using Sparse Representation Of Eeg Signals, Pouria Saidi

Electronic Theses and Dissertations

The human brain is unquestionably the most complex organ of the body as it controls and processes its movement and senses. A healthy brain is able to generate responses to the signals it receives, and transmit messages to the body. Some neural disorders can impair the communication between the brain and the body preventing the transmission of these messages. Brain Computer Interfaces (BCIs) are devices that hold immense potential to assist patients with such disorders by analyzing brain signals, translating and classifying various brain responses, and relaying them to external devices and potentially back to the body. Classifying motor imagery …

Unstable Ventilatory Control During Sleep After High Spinal Cord Injury: The Contribution Of Chemosensitivity And Hypoventilation, Amy Therese Bascom

Wayne State University Dissertations

ABSTRACT

UNSTABLE VENTILATORY CONTROL DURING SLEEP AFTER HIGH SPINAL CORD INJURY: THE CONTRIBUTION OF CHEMOSENSITIVITY AND HYPOVENTILATION

by

Amy T. Bascom

May 2015

Advisor: Dr. Harry G. Goshgarian

Major: Anatomy and Cell Biology

Degree: Doctor of Philosophy

A high prevalence of sleep-disordered breathing (SDB) after spinal cord injury (SCI) has been reported in the literature; however, the underlying mechanisms are not well understood. My studies had 2 aims: 1) to determine the effect of the withdrawal of the wakefulness drive to breathe on the degree of hypoventilation in SCI patients and able-bodied controls and 2) to determine the response of …

Shape Analysis Using Spectral Geometry, Jiaxi Hu

Wayne State University Dissertations

Shape analysis is a fundamental research topic in computer graphics and computer vision. To date, more and more 3D data is produced by those advanced acquisition capture devices, e.g., laser scanners, depth cameras, and CT/MRI scanners. The increasing data demands advanced analysis tools including shape matching, retrieval, deformation, etc. Nevertheless, 3D Shapes are represented with Euclidean transformations such as translation, scaling, and rotation and digital mesh representations are irregularly sampled. The shape can also deform non-linearly and the sampling may vary. In order to address these challenging problems, we investigate Laplace-Beltrami shape spectra from the differential geometry perspective, focusing more …

Unraveling The Genetic Mechanisms Involved In The Evolution And Development Of The Thoracic Appendages In Insects, Victor Medved

Wayne State University Dissertations

Insects display the greatest amount of structural and functional variation among animal groups, particularly in regard to their appendage morphology. These differences can range from the diverse pigmentation patterns between fore- and hindwings to changes in the size and shape of legs. The greatly enlarged jumping hind leg in crickets and grasshoppers is one of the best known illustrations of such diversity, representing a unique feature for the entire order of these insects (Orthoptera). Previous work from our lab has shown that the homeotic gene Ultrabithorax (Ubx) plays a key role in the enlargement of hind legs not only in …

The Role Of Crebh In Hepatic Energy Regulation Under Metabolic Stress, Roberto Mendez

Wayne State University Dissertations

Lipid metabolism is tightly regulated by nuclear receptors, transcription factors, and cellular enzymes in response to nutritional, hormonal, and stress signals. Hepatocyte specific, cyclic AMP responsive element-binding protein (CREBH) is a transcription factor that is preferentially expressed in the liver and localized in the endoplasmic reticulum (ER) membrane. CREBH is known to be activated by ER stress, inflammatory stimuli, and metabolic signals to regulate hepatic acute-phase response, lipid metabolism, and glucose metabolism. In my thesis research, I have characterized the roles and mechanisms of CREBH in these functions, as well as the overall phenotype of CrebH-null mice. I demonstrated that …

Inclusive Representation In Global Decision-Making Processses: Challenges Of Democracy, Sovereignty, And Liberatory Politics For Marginalized Groups, Laurel Dawn Sprague

Wayne State University Dissertations

This project proposes a framework for liberatory representation that institutionalizes processes to remove domination and meaningfully increasing respect and concern toward marginalized groups on issues that substantively affect them. It argues that decision-making processes that do not offer meaningful influence to those people who are most affected by particular decisions turn those who are supposed to be political equals into wards of dominant groups; essentially turning adults into political children. To meet ideals of political equality, liberatory processes for inclusive decision-making are required. The concept of political adulthood provides the foundation for an examination of current processes designed to bring …

Crebh, A Novel Liver Clock Keeper For Energy Metabolism, Ze Zheng

Wayne State University Dissertations

Circadian rhythms play crucial roles in orchestrating diverse physiological processes that are critical for health and disease. Cyclic AMP responsive element binding protein 3-like 3 (CREB3L3, also known as CREBH) is a liver-enriched, endoplasmic reticulum (ER)-tethered transcription factor known to regulate hepatic acute-phase response and energy homeostasis under stress conditions. Here, we demonstrate that CREBH is regulated by the circadian clock and functions as a diurnal regulator of hepatic lipid and glucose metabolism. CREBH is required to maintain circadian profiles of blood triglycerides, fatty acids, and glucose as well as hepatic glycogen storage. CREBH rhythmically regulates expression levels and amplitudes …

Hrd1 Partners In Endoplasmic Reticulum-Associated Degradation, Aaron Alexander Burr

Wayne State University Dissertations

Protein Quality Control (PQC) comprises cellular pathways that regulate the turnover of short-lived, misfolded proteins. A main component of PQC is Endoplasmic Reticulum (ER)-Associated Degradation (ERAD), which controls the degradation of proteins synthesized in the ER. Aberrations in ERAD have been linked to malignancies such as sarcomas, breast, and pancreatic carcinomas, as well as neurodegenerative disease. The machinery in this system is complex and while significant progress has been made to understand ERAD, it is not clear how the different components come together, or how they are regulated. HRD1 is a resident ubiquitin ligase that has been proposed as a …

Role Of Crebh In Endotoxin Mediated Modulation Of Hepatic Metabolism, Aditya Prakash Dandekar

Wayne State University Dissertations

Bacterial endotoxins can induce a variety of physiological changes in the host. This effect is not only restricted to inflammatory changes but also comprises metabolic changes in the host body. Lipopolysaccharide (LPS), one of the key components of the bacterial cell walls, is capable of triggering host metabolic changes. Hyperlipidemia usually accompanies with high endotoxin levels as well as inflammation. Lipid metabolism disorders are one of the common hallmarks of a patient with sepsis or high levels of endotoxin through diet. Previously, we have identified an endoplasmic reticulum (ER) anchored liver-specific transcription factor CREBH (cAMP-responsive element-binding protein, hepatocyte-specific), which is …

Soy Isoflavones Mediate Radioprotection Of Normal Lung Tissue By Modulating The Radiation-Induced Inflammatory Response, Lisa Marie Abernathy

Wayne State University Dissertations

Radiation-induced lung injury (RILI) is caused by an early inflammatory process triggered by damage to lung parenchyma, epithelial cells, vascular endothelial cells and stroma. Initially, oxidative injuries after radiation induce altered expression of pro-inflammatory cytokines. Infiltrating inflammatory cells are stimulated and activated, producing additional mediators, resulting in a cytokine cascade. The expansion and perpetual activation of inflammatory cells, as well as lung parenchyma, lead to clinical pneumonitis. Activated cells produce molecular mediators and growth factors that affect the proliferation and gene expression of lung fibroblasts. This process leads to increased collagen synthesis and deposition, eventually leading to the development of …

Investigating University Instructors’ Experiences And Uses Of Mobile Technology In Teaching And Learning In Saudi Arabia, Ibrahim K. Alali

Wayne State University Dissertations

The use of mobile technology in teaching and learning has become a worldwide phenomenon. Many university instructors in Saudi Arabia have also started using their own mobile technology in their teaching and for communication with students. But there is limited research on Saudi Arabian university faculty’s experiences of using mobile technology in their teaching practices. This mixed-method study adapted the R2D2 (Reading, Reflecting, Displaying, and Doing) model (Bonk & Zhang, 2006, 2008) to investigate faculty’s uses of mobile technology in Saudi Arabian universities, including both instructors’ own teaching and learners’ activities.

An online survey, with both Likert-scale items and open-ended …

Turning The Page: Fandoms, Multimodality, And The Transformation Of The 'Comic Book' Superhero, Matthew Alan Cicci

Wayne State University Dissertations

Superheroes are increasingly becoming more affiliated with film media than comic books. The amount of revenue generated, the formation of new fans, and the interests of comic publishers’ parent companies all suggest that superhero film adaptations are the medium most associated with the superhero character. Such a monumental shift in the distribution of superheroes—comic books were long the dominant medium of superhero characters—is indicative of ongoing media convergence practices; the success of these contemporary adaptations, from 1998 on, have not only caused the filmic superhero to eclipse the comic one, it has inevitably led to a rewriting of superhero comic …

Functional Analysis Of A Highly Conserved Cyclin, Cyclin Y, In Drosophila Melanogaster, Nermin Gerges

Wayne State University Dissertations

Cyclin Y is a highly conserved member of the Cyclin superfamily of proteins. In Drosophila the Cyclin Y gene (CycY) is required for progression through several stages of development but the specific pathways that Cyclin Y belongs to and that account for its requirement are not known. Studies in human and Drosophila cell lines have shown that membrane-localized Cyclin Y is required for phosphorylation of the wingless/Wnt co-receptor, arrow/LRP6, and for full activation of the canonical wingless/Wnt pathway. CycY null Drosophila, however, do not phenocopy loss-of-function mutations in canonical wingless pathway genes, suggesting that Cyclin Y may have additional roles …

The Acute And Chronic Effects Of Resveratrol On Renal Function And Blood Pressure, Kevin L. Gordish

Wayne State University Dissertations

We investigated the acute and chronic effects of resveratrol on renal function and blood pressure. We hypothesized that resveratrol would act as a renal vasodilator through a nitric oxide-dependent mechanism. In our acute studies, we found an intravenous bolus of resveratrol influenced changes in renal hemodynamics by increasing renal blood flow and decreasing renal vascular resistance. The mechanism of renal vasodilation was nitric oxide dependent and through a reduction of endogenous reactive oxygen species. Resveratrol-induced renal vasodilation was not influenced by COX metabolism and vasodilatory prostanoids. We found with continuous intravenous resveratrol infusion induced significant renal vasodilation while not altering …

Adipocyte-Induced Inflammation In Prostate Tumor Progression In Bone: Role Of Cxcr2 And Osteopontin, Aimalie Lynnette Hardaway

Wayne State University Dissertations

Abstract

Prostate cancer (PCa) is the second leading cause of cancer-related deaths among men. Evidence suggests that age and obesity, conditions associated with adipocyte accumulation in the bone marrow, are linked to increased risk of developing PCa and progressing to metastatic disease. Studies presented in this dissertation were based on the hypothesis that metastatic progression in bone is a result of a cooperative effort between bone marrow adipocytes, macrophages, osteoclasts, and PCa cells. We specifically focused on two adipocyte-supplied chemokines, CXCL1 and CXCL2, and bone marrow macrophage-secreted osteopontin as key drivers of pro-inflammatory environment in the bone marrow and important …

Islet Dysfunction In Diabetes, Syeda Khadija

Wayne State University Dissertations

Type 2 Diabetes [T2DM] is a chronic condition resulting from gradual failure of pancreatic beta cells to synthesize and secrete sufficient insulin to meet the metabolic demands and the inability of tissues [muscle, adipose and liver] to efficiently utilize the secreted insulin leading to an overall increase in blood glucose levels [hyperglycemia]. As indicated by recent estimates from the International Diabetes Federation, the prevalence of the disease in the year 2014 has risen to a record 387 million worldwide. The main objective of my project was to study the mechanisms involved in pancreatic beta cell dysfunction in diabetes, specifically in …

Investigation Of Whether Sedentary And Physically Active Conditions Lead To Altered Gabaergic Signaling In The Rvlm, Maryetta Donna Dombrowski

Wayne State University Dissertations

Investigation of whether sedentary and physically active conditions lead to altered GABAergic signaling in the RVLM

MD Dombrowski, and PJ Mueller

Department of Physiology, Wayne State University, School of Medicine, Detroit, MI

A sedentary lifestyle is a major risk for cardiovascular disease. Cardiovascular disease has been associated with elevated basal sympathetic nerve activity. The rostral ventrolateral medulla is a bilateral brainstem region that is an important for the control of resting and reflex control of sympathetic nerve activity and blood pressure. The activity of these neurons in this region is tonically inhibited by the neurotransmitter γ-butyric amino acid (GABA). Interestingly, …

Towards A Unified Model Of Sperm Chromatin Structure, Graham Johnson

Wayne State University Dissertations

Sperm possess several layers of information that are delivered to the oocyte alongside the paternal DNA. Examples of potential sperm borne molecular cues of probable use to the embryo include RNAs and local and global chromatin structure. To identify candidate sperm RNAs that likely reach the oocyte cytoplasm following fertilization patterns of transcript compartmentalization in the mature gamete were identified. Though all sperm RNAs exhibited a preferential peripheral enrichment, a subset of RNAs were identified in which this trend was reduced. These RNAs are thought to be embedded with perinuclear theca and are correlated with late spermatogenic transcription. Malat1, a …