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2014

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Articles 22591 - 22620 of 25854

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Genome-Wide Identification And Analysis Of Growth Regulating Factor Genes In Brachypodium Distachyon: In Silico Approaches, Ertuğrul Fi̇li̇z, İbrahi̇m Koç, Hüseyi̇n Tombuloğlu Jan 2014

Genome-Wide Identification And Analysis Of Growth Regulating Factor Genes In Brachypodium Distachyon: In Silico Approaches, Ertuğrul Fi̇li̇z, İbrahi̇m Koç, Hüseyi̇n Tombuloğlu

Turkish Journal of Biology

Growth-regulating factor (GRF) genes may play important roles for regulating growth and development in different plant tissues and organs. Here we report the first genome-wide analysis of the GRF gene family in Brachypodium. We performed in silico comparative analysis of GRF genes, including their structure, duplication in the genome, conserved motifs, and phylogenetic relationship. At the end of the study, 10 BdGRF genes were identified. The highest number of GRF genes was identified on chromosome 1 with 5 members, whereas the least number of genes (only 1 member) was found on chromosomes 2, 4, and 5. Of those, a single …

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Cover And Contents Jan 2014

Cover And Contents

Turkish Journal of Biology

No abstract provided.

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Production Of Biosurfactant By Pseudomonas Spp. Isolated From Industrial Waste In Turkey, Tayfun Kaya, Belma Aslim, Ergi̇n Kari̇ptaş Jan 2014

Production Of Biosurfactant By Pseudomonas Spp. Isolated From Industrial Waste In Turkey, Tayfun Kaya, Belma Aslim, Ergi̇n Kari̇ptaş

Turkish Journal of Biology

In this study, 26 Pseudomonas spp. were isolated from a stream polluted by factory waste and from petroleum-contaminated soil. The surface tension (ST) of the cultures was used as a criterion for the primary isolation of biosurfactant-producing bacteria. Biosurfactant production was quantified by ST reduction, critical micelle concentration (CMC), emulsification capacity (EC), and cell surface hydrophobicity (CSH). Two of the isolates, P. aeruginosa 78 and 99, produced rhamnolipid biosurfactant. The strains started rhamnolipid production in the logarithmic phase. They decreased the ST of the culture from 73 dyne/cm^2 to 29 and 33 dyne/cm^2, and the CMC of produced rhamnolipids were …

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Activity Of Antioxidant Enzymes During Induction Of Morphogenesis Of Fritillaria Meleagris In Bulb Scale Culture, Marija Petric, Sladana Jevremovic, Milana Trifunovic, Vojin Tadic, Snezana Milosevic, Angelina Subotic Jan 2014

Activity Of Antioxidant Enzymes During Induction Of Morphogenesis Of Fritillaria Meleagris In Bulb Scale Culture, Marija Petric, Sladana Jevremovic, Milana Trifunovic, Vojin Tadic, Snezana Milosevic, Angelina Subotic

Turkish Journal of Biology

We investigated the activity of 3 antioxidant enzymes: superoxide dismutase, catalase, and peroxidase during morphogenesis of Fritillaria meleagris L. in vitro in bulb scale culture. Bulb cultures of F. meleagris were established on nutrition medium supplemented with 1.0 mg L^{-1} thidiazuron. Bulbs were grown at standard (24 °C) and low temperatures (4 °C) or at an increased sucrose concentration (4.5%) during the first 6 weeks (pretreatments). All bulbs were then grown for 4 weeks at standard temperature on 2, 4-dichlorophenoxyacetic acid and kinetin or thidiazuron (at concentrations of 1 mg L^{-1} each) in order to examine the influence of the …

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Expression And Purification Of Porcine Akirin2 In Escherichia Coli, Xiaoling Chen, Zhiqing Huang, Bo Zhou, Huan Wang, Gang Jia, Jiayun Qiao Jan 2014

Expression And Purification Of Porcine Akirin2 In Escherichia Coli, Xiaoling Chen, Zhiqing Huang, Bo Zhou, Huan Wang, Gang Jia, Jiayun Qiao

Turkish Journal of Biology

Akirin2 is a recently discovered gene related to immune responses that also plays an important role in skeletal myogenesis. In this study, in order to scale up the production of recombinant porcine Akirin2 (pAkirin2), we report the expression and purification of a His-tagged version of recombinant pAkirin2 in Escherichia coli. The pAkirin2 is a polypeptide of 203 amino acids containing 42 rare codons. The recombinant pAkirin2 was expressed as an N-terminal fusion protein with His6 tag in E. coli Rosetta (DE3) host strain. The protein was purified by Ni-IDA affinity chromatography, yielding over 90% highly purified recombinant pAkirin2, with a …

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A Preliminary Analysis Of The Gene All0012 Of Anabaena Pcc 7120, Shen Tian, Sili Chen, Fengjiao Gong Jan 2014

A Preliminary Analysis Of The Gene All0012 Of Anabaena Pcc 7120, Shen Tian, Sili Chen, Fengjiao Gong

Turkish Journal of Biology

The genes coding for methyltransferase during the maturation of phycobilisomes in Synechococcus sp. strain PCC 7002 and Synechocystis sp. strain PCC 6803 were indentified and named CpcM. Using bioinformatics methods, it was found that a probable methyltransferase gene denoted all0012, because of its highly similarity, is likely to be the molecule that edits and modifies the \beta-subunit in Anabaena sp. strain PCC 7120. The present study was intended to analyze the probable functions of gene all0012. After bioinformatic analyses, we constructed an appropriate heterologous all0012 expression carrier and the characteristics of expression were evaluated by SDS-PAGE. Afterwards, a deletion mutant …

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Investigation Of Potential Virulence Genes And Antibiotic Resistance Characteristics Of Enterococcus Faecalis Isolates From Human Milk And Colostrum Samples, Si̇ne Özmen Toğay, Ayhan Temi̇z, Ayten Çelebi̇, Leyla Açik, Siddi̇ka Songül Yalçin Jan 2014

Investigation Of Potential Virulence Genes And Antibiotic Resistance Characteristics Of Enterococcus Faecalis Isolates From Human Milk And Colostrum Samples, Si̇ne Özmen Toğay, Ayhan Temi̇z, Ayten Çelebi̇, Leyla Açik, Siddi̇ka Songül Yalçin

Turkish Journal of Biology

Enterococci may improve the typical taste and flavor of fermented foods through their proteolytic and lipolytic activities. However, some enterococcal strains are recognized as nosocomial pathogens, which have virulence genes and resistance to certain antibiotics. Enteroccocci are also found in human milk microflora. The aim of this study was to investigate the potential virulence genes and antibiotic resistance characteristics of Enterococcus faecalis isolates from human milk and colostrum samples. In total, 23 Enterococcus faecalis strains were identified from human milk and colostrum samples. Antibiotic-resistant E. faecalis isolates were determined using the disk diffusion method. Vancomycin resistance genes (vanA, vanB) and …

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Calcitriol Modulates The Effects Of The Supernatants Of Bone-Marrow-Derived Mesenchymal Stem Cells On Neutrophil Functions, Hadi Esmaili Gouvarchin Galeh, Norouz Delirezh, Seyyed Meysam Abtahi Froushani, Nahideh Afzale Ahangaran Jan 2014

Calcitriol Modulates The Effects Of The Supernatants Of Bone-Marrow-Derived Mesenchymal Stem Cells On Neutrophil Functions, Hadi Esmaili Gouvarchin Galeh, Norouz Delirezh, Seyyed Meysam Abtahi Froushani, Nahideh Afzale Ahangaran

Turkish Journal of Biology

Mesenchymal stem cells (MSCs) in bone marrow form a niche that has inevitable interactions with neutrophils. Moreover, previous documents have shown that calcitriol has an important role in regulating the cell growth of MSCs. This study set out to investigate the effects of calcitriol on the interaction between bone-marrow-derived MSCs and neutrophils. MSCs were isolated from the bone marrow of rats and pulsed with different concentrations of calcitriol (50, 100, and 200 nM) for different periods of time (24, 48, and 72 h). As the next step, the supernatants of MSCs cocultured with neutrophils for 4 h and neutrophil functions …

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Identification Of An Aflp Marker Linked With Yellow Rust Resistance In Wheat (Triticum Aestivum L.), Handan Balta, Özge Karakaş Meti̇n, Funda Şentürk Akfirat, Fahri̇ye Ertuğrul, Semra Hasançebi̇, Yildiz Aydi̇n, Kadi̇r Akan, Zafer Mert, Müge Türet, Ahu Altinkut Uncuoğlu Jan 2014

Identification Of An Aflp Marker Linked With Yellow Rust Resistance In Wheat (Triticum Aestivum L.), Handan Balta, Özge Karakaş Meti̇n, Funda Şentürk Akfirat, Fahri̇ye Ertuğrul, Semra Hasançebi̇, Yildiz Aydi̇n, Kadi̇r Akan, Zafer Mert, Müge Türet, Ahu Altinkut Uncuoğlu

Turkish Journal of Biology

An important application of molecular markers in plant systems involves improvement in the efficiency of conventional plant breeding by carrying out indirect selection through molecular markers linked to the traits of interest. AFLP analysis was used to identify molecular markers associated with yellow rust resistance in wheat (Triticum aestivum L.) in this study. DNA isolated from the selected yellow rust tolerant and susceptible F_2 individuals derived from a cross between Izgi2001 (resistant) and ES14 (susceptible) at seedling and adult stage was used for bulked segregant analysis combined with AFLP. From the screening of 34 PstI/MseI AFLP primer combinations, the AFLP …

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Y Chromosome Analysis Of Native Turkish Cattle Breeds By Microsatellite Markers, Yusuf Özşensoy, Ercan Kurar, Zafer Bulut, Mehmet Ni̇zamlioğlu Jan 2014

Y Chromosome Analysis Of Native Turkish Cattle Breeds By Microsatellite Markers, Yusuf Özşensoy, Ercan Kurar, Zafer Bulut, Mehmet Ni̇zamlioğlu

Turkish Journal of Biology

The aim of this study was to determine phylogenetic relationships of 6 native cattle breeds of Turkey using 7 Y chromosome-specific microsatellite DNA markers. DNA samples were isolated from Anatolian Black, Anatolian Grey, South Anatolian Red, Native Southern Anatolian Yellow, East Anatolian Red, and Zavot cattle using a standard phenol/chloroform method. PCR products were separated by capillary electrophoresis and marker genotypes were determined. A total of 41 different alleles were observed. The mean allele number was 5.86 and mean F_{IS} value was 0.427 for all populations. The INRA189 locus was monomorphic for all populations except for South Anatolian Red. UMN0307 …

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Prothoracicotropic Hormone-Producing Neurosecretory Neurons And Antioxidative Defense In Midgut Of Lymantria Dispar In Trophic Stress, Vesna Peric Mataruga, Milena Vlahovic, Marija Mrdakovic, Dajana Todorovic, Dragana Matic, Anja Gavrilovic, Larisa Ilijin Jan 2014

Prothoracicotropic Hormone-Producing Neurosecretory Neurons And Antioxidative Defense In Midgut Of Lymantria Dispar In Trophic Stress, Vesna Peric Mataruga, Milena Vlahovic, Marija Mrdakovic, Dajana Todorovic, Dragana Matic, Anja Gavrilovic, Larisa Ilijin

Turkish Journal of Biology

As a very invasive insect species, Lymantria dispar is adaptable and sensitive to a changing environment. In insects the neuroendocrine system first reacts to stress by production of prothoracicotropic neurohormones (PTTH) that control ecdysteroid synthesis (morphogenetic and stress hormones). In this article, we report changes in the L2' brain neurosecretory neurons that synthesize PTTH in L. dispar larvae after feeding on locust tree leaves (Robinia pseudoacacia), an unsuitable host plant. Groups of larvae (n = 20 per experimental group) were offered this in comparison with oak leaves (Quercus robur), a suitable control diet, for 3 days after molting into the …

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Cover And Contents Jan 2014

Cover And Contents

Turkish Journal of Biology

No abstract provided.

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Efficiency Of Using Rapd And Issr Markers In Evaluation Of Genetic Diversity In Sugar Beet, Vusala Izzatullayeva, Zeynal Akparov, Sevda Babayeva, Javid Ojaghi, Mehraj Abbasov Jan 2014

Efficiency Of Using Rapd And Issr Markers In Evaluation Of Genetic Diversity In Sugar Beet, Vusala Izzatullayeva, Zeynal Akparov, Sevda Babayeva, Javid Ojaghi, Mehraj Abbasov

Turkish Journal of Biology

Random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR) markers were used for comparative analysis of genetic variation in 42 sugar beet accessions. A total of 24 polymorphic primers (12 RAPD and 12 ISSR) were used. The RAPD primers generated 204 amplification products and the ISSR primers produced 178 fragments, 190 and 173 of which were polymorphic, respectively. The average polymorphism level with the ISSR markers (97.2%) was higher than that with the RAPD primers (93%). High genetic diversity indices for both marker types (0.86 for RAPD and 0.91 for ISSR) suggested that these methods were equally effective in …

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Herbicide-Tolerant Sugarcane (Saccharum Officinarum L.) Plants: An Unconventional Method Of Weed Removal, Idrees Ahmad Nasir, Bushra Tabassum, Zahida Qamar, Muhammad Aslam Javed, Muhammad Tariq, Abdul Munim Farooq, Shahid Javed Butt, Abdul Qayyum, Tayyab Husnain Jan 2014

Herbicide-Tolerant Sugarcane (Saccharum Officinarum L.) Plants: An Unconventional Method Of Weed Removal, Idrees Ahmad Nasir, Bushra Tabassum, Zahida Qamar, Muhammad Aslam Javed, Muhammad Tariq, Abdul Munim Farooq, Shahid Javed Butt, Abdul Qayyum, Tayyab Husnain

Turkish Journal of Biology

Unnecessary weed growth in sugarcane fields forces plants to compete for nutrients and sunlight for survival, which most often leads to significant yield losses. As chemical herbicides cannot differentiate between crop plants and weeds, the development of herbicide-tolerant crops is anticipated. Four sugarcane varieties, CPF-234, CPF-213, HSF-240, and CPF-246, were used to develop glyphosate herbicide tolerance. A glyphosate-tolerant gene of 1368 bp cloned directionally under the 35S promoter with the \Beta-glucuronidase (GUS) reporter gene was used as the transgene. Through the biolistic transformation of sugarcane, calli of all cultivars were transformed with glyphosate-tolerant gene constructs. Efficient regeneration conditions were optimized …

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Characterization Of Dehydrin Ahdhn From Mediterranean Saltbush (Atriplex Halimus), Monther Taha Sadder, Abdullah Abdulaziz Al-Doss Jan 2014

Characterization Of Dehydrin Ahdhn From Mediterranean Saltbush (Atriplex Halimus), Monther Taha Sadder, Abdullah Abdulaziz Al-Doss

Turkish Journal of Biology

Dehydrins are a group of proteins whose expression is associated with plant abiotic stresses and seed maturation. The full AhDHN dehydrin gene from Mediterranean saltbush (Atriplex halimus) was cloned and characterized. The AhDHN amino acid sequence revealed 1 S-segment, 3 K-segments (K1, K2, K3), and a transmembrane pore-lining signature. It is a 26.77-kDa protein with a predicted isoelectric point of 6.14. Phylogenetic analysis of the AhDHN protein clustered it with other proteins from related plants belonging to the family Amaranthaceae (Atriplex canescens, Spinacia oleracea, Suaeda salsa, and Suaeda glauca). The AhDHN protein sequence revealed a deletion that was 102 amino …

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Enhanced Enzymatic Xylose/Cellulose Fractionation From Alkaline Liquor-Pretreated Corn Cob By Surfactant Addition And Separate Fermentation To Bioethanol, Yefu Chen, Xinxin Zhang, Shijie Zhang, Weijun Qin, Changhui Guo, Xuewu Guo, Dongguang Xiao Jan 2014

Enhanced Enzymatic Xylose/Cellulose Fractionation From Alkaline Liquor-Pretreated Corn Cob By Surfactant Addition And Separate Fermentation To Bioethanol, Yefu Chen, Xinxin Zhang, Shijie Zhang, Weijun Qin, Changhui Guo, Xuewu Guo, Dongguang Xiao

Turkish Journal of Biology

Xylanase xylose/cellulose fractionation can efficiently improve the utilization of xylose and cellulose. Improvement of xylanase performance during hemicellulose hydrolysis can increase xylose/cellulose fractionation efficiency. To utilize xylose and cellulose efficiently, an enzymatic xylose/cellulose fractionation and a separate fermentation bioethanol process were performed. Alkaline liquor-pretreated corn cob was subjected to xylose/cellulose fractionation with xylanase in the presence or absence of the following mixed surfactants: polyoxyethylene(20)-sorbitan-monooleate (0.15% v/v), polyethylene glycol 6000 (0.15% w/v), bovine serum albumin (0.15% w/v), and rhamnolipid (0.005% v/v). The hemicellulose hydrolysis yield (HHY) in the presence of mixed surfactants was 70.5%, which was 66% higher than that of …

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Preconditioning Effect Of Cytokinins On In Vitro Multiplication Of Embryonic Node Of Grass Pea (Lathyrus Sativus L.) Cultivar Gürbüz, Surendra Barpete, Muhammad Aasim, Khalid Mahmood Khawar, Sancar Fati̇h Özcan, Sebahatti̇n Özcan Jan 2014

Preconditioning Effect Of Cytokinins On In Vitro Multiplication Of Embryonic Node Of Grass Pea (Lathyrus Sativus L.) Cultivar Gürbüz, Surendra Barpete, Muhammad Aasim, Khalid Mahmood Khawar, Sancar Fati̇h Özcan, Sebahatti̇n Özcan

Turkish Journal of Biology

Lathyrus sativus L. (grass pea) is an important grain legume crop grown all over the world. It is very recalcitrant and difficult to regenerate under in vitro conditions. The present study determined the effect of preconditioning of cytokinins [10 and 20 mg L^{-1} thidiazuron (TDZ) and 2-isopentenyl adenine (2iP)] and postconditioning of dissimilar levels of TDZ-indole-3-butyric acid (IBA) and 2iP-IBA on in vitro shoot multiplication using embryonic node explants. The results showed that preconditioning followed by postconditioning on Murashige and Skoog (MS) medium with 0.25, 0.50, 0.75, and 1.0 mg L^{-1} 2iP or TDZ combined with 0.10 mg L^{-1} IBA …

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Melatonin Is Effective In Reducing Stress-Induced Organ Damage In Wistar Albino Rats, Mukaddes Eşrefoğlu, Ayşi̇n Akinci Bak, Hülya Elbe, Eli̇f Taşlidere, Asli Çeti̇n, Burhan Ateş Jan 2014

Melatonin Is Effective In Reducing Stress-Induced Organ Damage In Wistar Albino Rats, Mukaddes Eşrefoğlu, Ayşi̇n Akinci Bak, Hülya Elbe, Eli̇f Taşlidere, Asli Çeti̇n, Burhan Ateş

Turkish Journal of Biology

In the present study, we tried to investigate the effects of melatonin, a novel antioxidant and a potent free radical scavenger, in stress-induced cerebral, cerebellar, cardiac, and hepatic oxidative damage using microscopic and biochemical analysis. A total of 32 male Wistar albino rats were divided into control, stress, stress + saline, and stress + melatonin groups. The rats from the stress groups were exposed to high stress conditions of starvation, immobilization, and cold exposure. The rats from the stress + melatonin group received melatonin daily at 20 mg/kg body weight intraperitoneally for 7 days. At the end of the experiment, …

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Expression And Purification Of Porcine Pid1 Gene In Escherichia Coli, Huan Wang, Xiaoling Chen, Zhiqing Huang, Bo Zhou, Gang Jia, Guangmang Liu, Hua Zhao Jan 2014

Expression And Purification Of Porcine Pid1 Gene In Escherichia Coli, Huan Wang, Xiaoling Chen, Zhiqing Huang, Bo Zhou, Gang Jia, Guangmang Liu, Hua Zhao

Turkish Journal of Biology

In this study, in order to scale up the production of recombinant porcine phosphotyrosine interaction domain containing 1 (pPID1), a pET-28a (+)-pPID1 expression plasmid was constructed and transformed into Escherichia coli Rosetta (DE3). The recombinant pPID1 was then purified and identified by western blotting, and was also analyzed in vitro for its function. The recombinant protein was tagged with only a His6 tag at its C-terminus, which could be conveniently purified by affinity column. The protein could be induced for efficient expression with 0.75 mM IPTG for 8 h at 30 °C, yielding approximately 3 mg/L. In vitro biological activity …

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Evaluation Of Orange Peel, An Industrial Waste, For The Production Of Aspergillus Sojae Polygalacturonase Considering Both Morphology And Rheology Effects, Ni̇han Göğüş, Bengi̇ Hakgüder Taze, Hande Demi̇r, Canan Tari, Sevcan Ünlütürk, Marcelo Fernandez Lahore Jan 2014

Evaluation Of Orange Peel, An Industrial Waste, For The Production Of Aspergillus Sojae Polygalacturonase Considering Both Morphology And Rheology Effects, Ni̇han Göğüş, Bengi̇ Hakgüder Taze, Hande Demi̇r, Canan Tari, Sevcan Ünlütürk, Marcelo Fernandez Lahore

Turkish Journal of Biology

Orange peel is an agroindustrial waste rich in pectin and known to be an inducer for pectinase production. The use of this low-cost substrate for the production of an industrially important enzyme, polygalacturonase (PG), can be an alternative way to turn this waste into a value-added product, contributing to the reduction of environmental waste disposal problems. Enzyme productions by fungal microorganisms are affected by environmental and nutritional factors, demanding the determination of optimum conditions for maximum enzyme production with the desired fungal morphology and broth rheology. Therefore, complex and additional carbon sources were optimized with respect to PG production by …

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Impact Of Salt Stress On Photosystem Ii Efficiency And Antioxidant Enzyme Activities Of Safflower (Carthamus Tinctorius L.) Cultivars, Şeküre Çulha Erdal, Hüsnü Çakirlar Jan 2014

Impact Of Salt Stress On Photosystem Ii Efficiency And Antioxidant Enzyme Activities Of Safflower (Carthamus Tinctorius L.) Cultivars, Şeküre Çulha Erdal, Hüsnü Çakirlar

Turkish Journal of Biology

This study was conducted to determine the tolerance of safflower (Carthamus tinctorius L.) cultivars (Dinçer, Remzibey-05, and Yenice) against salt stress based on some physiological and biochemical parameters at the vegetative stage. Eighteen-day-old plants were subjected to salt stress [0 (control), 75, 150, 225, and 300 mM NaCl concentrations] for 12 days, which led to a significant decrease in growth parameters (stem growth, fresh weight, and relative water content) and the photosynthetic pigment contents of the safflower cultivars. The photochemical activities of photosystem II of the cultivars were negatively affected by salinity, especially at the highest concentration (300 mM). Salt …

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Cover And Contents Jan 2014

Cover And Contents

Turkish Journal of Biology

No abstract provided.

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Optimization Of E. Coli Culture Conditions For Efficient Dna Uptake By Electroporation, Irfan Ahmad, Tehseen Rubbab, Farah Deeba, Syed Muhammad Saqlan Naqvi Jan 2014

Optimization Of E. Coli Culture Conditions For Efficient Dna Uptake By Electroporation, Irfan Ahmad, Tehseen Rubbab, Farah Deeba, Syed Muhammad Saqlan Naqvi

Turkish Journal of Biology

Bacterial transformation is a significant breakthrough in the field of molecular biology for cloning purposes. A variety of chemical and physical methods are utilized for enhancing bacterial competence. The present study was designed, via the consolidation of chemical and physical methods, to improve competence of cells for high transformation efficiency up to 10^9 transformants/µg of DNA. LB and SOC media and their supplemented combinations with osmotic agents (sucrose and glycine) were used to prepare electrocompetent cells of E. coli DH5\alpha at temperatures of 25 and 37 °C, which were then subjected to electroporation. The results showed that cells grown in …

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Characterization Of The Human Sialidase Neu4 Gene Promoter, Volkan Seyrantepe, Murat Delman Jan 2014

Characterization Of The Human Sialidase Neu4 Gene Promoter, Volkan Seyrantepe, Murat Delman

Turkish Journal of Biology

There are 4 different sialidases that have been described in humans: lysosomal (Neu1), cytoplasmic (Neu2), plasma membrane (Neu3), and lysosomal/mitochondrial (Neu4). Previously, we have shown that Neu4 has a broad substrate specificity and is active against glyco-conjugates, including G_{M2} ganglioside, at the acidic pH of 3.2. An overexpression of Neu4 in transfected neuroglia cells from a Tay-Sachs patient shows a clearance of accumulated G_{M2}, indicating the biological importance of Neu4. In this paper, we aimed to characterize a minimal promoter region of the human Neu4 gene in order to understand the molecular mechanism regulating its expression. We cloned 7 different …

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Effect Of Polyamines On In Vitro Anther Cultures Of Carrot (Daucus Carota L.)., Krystyna Górecka, Waldemar Kiszczak, Dorota Krzyzanowska, Urszula Kowalska, Agata Kapuscinska Jan 2014

Effect Of Polyamines On In Vitro Anther Cultures Of Carrot (Daucus Carota L.)., Krystyna Górecka, Waldemar Kiszczak, Dorota Krzyzanowska, Urszula Kowalska, Agata Kapuscinska

Turkish Journal of Biology

This study examines the influence of the polyamines putrescine and spermidine on the efficiency of androgenesis in anther cultures of 2 carrot cultivars, Kazan F_1 and Narbonne F_1, and the effect of putrescine on the process of plant regeneration from androgenetic embryos. In the Kazan F_1 variety, an increase in the number of obtained embryos was achieved using each of the 2 polyamines separately and in combination. In contrast, no beneficial effects of polyamines on the efficiency of androgenesis were observed in the Narbonne F_1 variety. Putrescine added to the regeneration medium increased the number of obtained plants. For embryos …

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Phylogenetic Analysis And Characterization Of An Alkane-Degrading Yeast Strain Isolated From Oil-Polluted Soil, Ortansa Csutak, Tatiana Vassu Jan 2014

Phylogenetic Analysis And Characterization Of An Alkane-Degrading Yeast Strain Isolated From Oil-Polluted Soil, Ortansa Csutak, Tatiana Vassu

Turkish Journal of Biology

The isolation and phylogenetic analysis of new yeast strains represent an important basis for the development of practical applications in biotechnology, industry, and bioremediation. The yeast strain RG1 was isolated from oil-polluted soil and characterized using morphological observations and the API 20C AUX system. The amplified ITS1-5.8S rDNA-ITS2 region had 653 bp and the restriction profiles obtained with Cfo I, Dde I, Hae III, Hinf I, and Hpa II endonucleases showed high similarities with those from Rhodotorula glutinis reference strains. The phylogenetic tree based on the Cfo I patterns confirmed the classification of the strain RG1 as belonging to the …

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Large-Scale Purification Of A Bacteriocin Produced By Leuconostoc Mesenteroides Subsp. Cremoris Using Diatomite Calcium Silicate, Hali̇l Dündar, Ömür Çeli̇kbiçak, Beki̇r Sali̇h, Tahsi̇n Faruk Bozoğlu Jan 2014

Large-Scale Purification Of A Bacteriocin Produced By Leuconostoc Mesenteroides Subsp. Cremoris Using Diatomite Calcium Silicate, Hali̇l Dündar, Ömür Çeli̇kbiçak, Beki̇r Sali̇h, Tahsi̇n Faruk Bozoğlu

Turkish Journal of Biology

Leuconostoc mesenteroides subsp. cremoris strain W3, isolated from wine, was previously found to produce a bacteriocin with inhibitory activity towards malolactic bacteria and hence inhibit malolactic fermentation in model wine medium. The bacteriocin, which we termed mesentericin W3, was purified by multistep chromatography and found in a previous study to be similar to mesentericin Y105, albeit with a low recovery (3.2%). In this study, we aimed to achieve large-scale isolation of mesentericin W3 by adsorption of the bacteriocin from culture supernatant onto Micro-Cel (diatomite calcium silicate) and then by subsequent desorption. Water-soluble surfactants including Tween 80, Triton X-100, sodium deoxycholate, …

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Cloning, Purification, And Characterization Of A Thermophilic Ribulokinase From Anoxybacillus Kestanbolensis Ac26sari, Müslüm Tokgöz, Kadri̇ye İnan, Ali̇ Osman Beldüz, Öznur Gedi̇kli̇, Sabri̇ye Çanakçi Jan 2014

Cloning, Purification, And Characterization Of A Thermophilic Ribulokinase From Anoxybacillus Kestanbolensis Ac26sari, Müslüm Tokgöz, Kadri̇ye İnan, Ali̇ Osman Beldüz, Öznur Gedi̇kli̇, Sabri̇ye Çanakçi

Turkish Journal of Biology

The gene encoding ribulokinase araB from Anoxybacillus kestanbolensis AC26Sari was cloned and sequenced. The recombinant protein was expressed in Escherichia coli BL21 under the control of isopropyl-\beta-D-thiogalactopyranoside-inducible T7 promoter. The enzyme, designated as AC26RK, was purified with the MagneHis Protein Purification System. The molecular mass of the native protein, as determined by SDS-PAGE, was about 61 kDa. AC26RK was active throughout a broad pH (pH 5.0-10.0) and temperature (50-75 °C) range, and it had an optimum pH of 9.0 and optimum temperature of 60 °C. The enzyme displayed about 90%-100% of its original activities after a 30-min incubation at a …

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Cloning And Expression Analysis Of 1-Deoxy-D-Xylulose-5-Phosphate Synthase Gene From The Medicinal Plant Conyza Blinii H.Lév., Rong Sun, Shan Liu, Jing-Lei Gao, Zi-Zhong Tang, Hui Chen, Cheng-Lei Li, Qi Wu Jan 2014

Cloning And Expression Analysis Of 1-Deoxy-D-Xylulose-5-Phosphate Synthase Gene From The Medicinal Plant Conyza Blinii H.Lév., Rong Sun, Shan Liu, Jing-Lei Gao, Zi-Zhong Tang, Hui Chen, Cheng-Lei Li, Qi Wu

Turkish Journal of Biology

Conyza blinii H.Lév. is a traditional Chinese medicinal plant that is distributed mainly in southwestern Sichuan and northern Yunnan. Its characteristic product is blinin, which has, among other properties, antigastric ulcer activity, and can serve as a quality-control standard for such medicine. The problem is that C. blinii only produces low yields of blinin. As a diterpene, blinin is likely formed by the methylerythritol phosphate pathway. While 1-deoxy-D-xylulose-5-phosphate synthase (DXS) is the first rate-limiting enzyme in diterpenoid biosynthesis, it is a switch in the pathway. The DXS gene was successfully cloned and characterized from C. blinii by homologous cloning and …

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Phylogenetic Analysis Of Peste Des Petits Ruminants Virus From Outbreaks In Turkey During 2008-2012, Leyla Güler, Murat Şevi̇k, Mustafa Hasöksüz Jan 2014

Phylogenetic Analysis Of Peste Des Petits Ruminants Virus From Outbreaks In Turkey During 2008-2012, Leyla Güler, Murat Şevi̇k, Mustafa Hasöksüz

Turkish Journal of Biology

Peste des petits ruminants (PPR) is an important viral disease of sheep and goats and is endemic in all regions of Turkey. In this study, PPR virus infection was investigated by RT-PCR assay based on the fusion (F) gene in PPR-suspected sheep and goat samples. PPR virus RNA was detected in 65 small ruminants (51 sheep, 14 goats) from independent outbreaks during 2008-2012 in provinces in the central and Mediterranean regions and the central-west part of the Aegean region in Turkey. The virus was detected in an aborted sheep fetus sample by RT-PCR, and diagnosis was also confirmed by virus …

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