Digital Commons Network^™

Expression And Splicing Of Alzheimer’S Disease Risk Gene Phosphatidylinositol-Binding Clathrin Assembly Protein, Ishita Parikh

Theses and Dissertations--Physiology

Recent Genome Wide Association Studies (GWAS) have identified a series of single nucleotide polymorphism (SNP)s that are associated with Alzheimer’s disease (AD). One of the SNPs, rs3851179 (G/A), is near the gene phosphatidylinositol-binding clathrin assembly protein (PICALM). To evaluate whether this SNP is associated with PICALM expression, we quantified PICALM mRNA in 56 brain cDNA samples. Using linear regression analysis, we analyzed PICALM expression relative to rs3851179, AD status, and cell type specific markers. An association was detected between rs3851179 and PICALM, microvessel mRNA, glial fibrillary acidic protein (GFAP) mRNA, and synaptophysin (SYN) mRNA. To gain clarity …

Nucleoside Reverse Transcriptase Inhibitors Are Anti-Inflammatory And Target Dry Age-Related Macular Degeneration, Benjamin J. Fowler

Theses and Dissertations--Physiology

Age-related macular degeneration (AMD) is a principal cause of blindness in the United States and other industrialized nations. An estimated 10 million Americans are afflicted with AMD, which is comparable in scope to the 12 million living with cancer, or the 5 million with Alzheimer’s disease. The prevalence of AMD steadily increases with age, affecting 2% of the population at age 40, and one in four people by age 80. For reasons that are not fully understood, AMD is more common in lightly-pigmented and female populations. Treatment of AMD is largely an unmet need: There are no FDA approved therapies …

Attenuating Trigeminal Neuropathic Pain By Repurposing Pioglitazone And D-Cycloserine In The Novel Trigeminal Inflammatory Compression Mouse Model, Danielle N. Lyons

Theses and Dissertations--Physiology

Approximately 22% of the United States population suffers from a chronic orofacial pain condition. One such condition is known as trigeminal neuropathic pain frequently reported as continuous aching and burning pain, often accompanied by intermittent electrical shock-like sensations. Dental procedures or trauma are known causes of peripheral trigeminal nerve injury and inflammation. Patients who have this type of facial pain also suffer from emotional distress. For these reasons, trigeminal neuropathic pain needs to be studied in more detail to improve the understanding of the etiology and maintenance of this condition, as well as to develop effective treatment strategies. The first …

Trpa1 Channels In Cochlear Supporting Cells Regulate Hearing Sensitivity After Noise Exposure, Alejandra C. Velez-Ortega

Theses and Dissertations--Physiology

TRPA1 channels are sensors for noxious stimuli in a subset of nociceptive neurons. TRPA1 channels are also expressed in cells of the mammalian inner ear, but their function in this tissue remains unknown given that Trpa1^–/– mice exhibit normal hearing, balance and sensory mechanotransduction. Here we show that non-sensory (supporting) cells of the hearing organ in the cochlea detect tissue damage via the activation of TRPA1 channels and subsequently modulate cochlear amplification through active cellshape changes.

We found that cochlear supporting cells of wild type but not Trpa1^–/– mice generate inward currents and robust long-lasting Ca²⁺ responses …

Identification Of Soils On Firefighter Turnout Gear From The Philadelphia Fire Department, Tabitha N. Huston

Theses and Dissertations--Retailing and Tourism Management

The purpose of this research was to identify the composition of soils on firefighter turnout gear from the Philadelphia Fire Department and to determine which soils contain hazardous materials. The objective of the research was to identify the composition of soils remaining on the firefighter turnout gear to enable the industry to design an effective cleaning procedure for removal of soils. A pilot study was conducted on hoods from the Philadelphia fire department to evaluate the test methods that would be used to identify the composition of soils. Soils that had been identified from previous studies were targeted in the …

Evaluation Of Pcr-Based Methods For Rapid, Accurate Detection And Monitoring Of Verticillium Dahliae In Woody Hosts By Real-Time Polymerase Chain Reaction, Baker Diwan Getheeth Aljawasim

Theses and Dissertations--Plant Pathology

Verticillium wilt, caused by Verticillium dahliae Kleb, is one of the most economically important diseases of woody hosts such as ash (Fraxinus spp.), sugar maple (Acer saccharum), and redbud (Cercis canadensis). The causal agent has a broad host range, including not only woody hosts but also important vegetable and field crops, and it is distributed worldwide. Diagnosis of V. dahliae in infected woody hosts is often based on the occurrence of vascular discoloration and time-consuming isolation. However, not all woody hosts exhibit vascular discoloration symptoms, and not all vascular discoloration symptoms are due to infection …

The Effects Of Mountaintop Removal Mining And Valley Fills On Stream Salamander Communities, Brenee' Lynn Muncy

Theses and Dissertations--Forestry and Natural Resources

Mountaintop removal mining and valley filling (MTR/VF) is a common form of land conversion in Central Appalachia and threatens the integrity of stream ecosystems. We investigated the effects of MTR/VF on stream salamander occupancy probabilities and community structure by conducting area constrained active searches for stream salamanders within intermittent streams located in mature forest (i.e., control) and those impacted by MTR/VF. During March to June of 2013, we detected five stream salamander species (Desmognathus fuscus, D. monticol, Eurycea cirrigera, Pseudotriton ruber, and Gyrinophilus porphyriticus) and found that the probability of occupancy was greatly reduced in MTR/VF streams compared …

Influence Of Spoil Type On Afforestation Success And Hydrochemical Function On A Surface Coal Mine In Eastern Kentucky, Kenton L. Sena

Theses and Dissertations--Forestry and Natural Resources

Surface coal mining in Appalachia has contributed to a suite of ecological impacts, both terrestrial and aquatic. Conventional reclamation in Appalachia leads to the development of hay/pasture systems dominated by nonnative grasses and legumes, with soils that are chemically and physically unfavorable to native tree growth. Several studies have shown that more weathered minespoils provide a better growth medium than unweathered spoils in Appalachia. Spoil segregation plots were constructed on Bent Mountain in Pike County, KY, to compare the suitability of three mine spoil types (BROWN weathered sandstone, GRAY unweathered sandstone, and MIXED sandstones and shales). In 2013 (after nine …

Use Of Genomic Tools To Discover The Cause Of Champagne Dilution Coat Color In Horses And To Map The Genetic Cause Of Extreme Lordosis In American Saddlebred Horses, Deborah G. Cook

Theses and Dissertations--Veterinary Science

Champagne dilution of coat color in horses is caused by dominant gene action. Three sire families were identified as segregating for this trait. Genome wide linkage analysis using 104 microsatellite DNA markers was used to map the gene to ECA14 (LOD > 11.0). Four genes, namely SPARC, SLC36A1, SLC36A2 and SLC36A3, were selected from the region implicated by linkage and their exons sequenced. DNA sequences were compared for two homozygotes for Champagne dilution, two heterozygotes and two horses without dilution. A single base change in exon 2 of SLC36A1 was found unique to horses exhibiting Champagne dilution. This change in base …

Modulation Of Type-I Interferon Mediated Immune Response: A Novel Innate Immune Evasion Strategy Of Equine Herpesvirus 1, Sanjay Sarkar

Theses and Dissertations--Veterinary Science

Equine herpesvirus-1 (EHV-1) is one of the major viral pathogens causing respiratory disease, abortion, perinatal mortality and neurologic disease among horses resulting in significant economic losses to the equine industry. The virus can also remain latent in the horses and recrudesce at any time. Type-I interferons (IFNs) act as a first line of defense against many viral infections.

In this study we investigated the type-I IFN response against the neuropathogenic T953 strain of EHV-1 in equine endothelial cells (EECs). The results showed that after a transient induction of IFN-β mRNA as well as protein at an early time (3h) post …

Monitoring The Influence Of Acid Deposition On Soil And Implications To Forest Health In The Daniel Boone National Forest, Tyler M. Sanderson

Theses and Dissertations--Forestry and Natural Resources

Combustion of fossil fuels has contributed to many environmental problems including acid deposition. The Clean Air Act (CAA) was created to reduce ecological problems by cutting emissions of sulfur and nitrogen. Reduced emissions and rainfall concentrations of acidic ions have been observed since the enactment of the CAA, but soils continue to receive some acid inputs. Soils sensitive to acid deposition have been found to have low pH, a loss of base cations and a shift in the mineral phase controlling the activity of Al³⁺ and/or SO₄^2-. If inputs continue, soil may be depleted of base …

Anti-Müllerian Hormone In Stallions And Mares: Physiological Variations, Clinical Applications, And Molecular Aspects, Anthony N.J. Claes

Theses and Dissertations--Veterinary Science

Anti-Müllerian hormone (AMH) is a homodimeric glycoprotein that is best known for its role in regression of the Müllerian duct in the male fetus. Accumulating evidence indicates that AMH also has an important role during different physiological processes after birth. In contrast to other species, relatively little is known about AMH in the horse. In chapter one, developmental and seasonal changes in serum AMH concentrations in male horses were determined, and the use of AMH for determination of retained cryptorchid testes was established. In chapter two, the interrelationship between plasma AMH concentrations, antral follicle counts (AFC), and age in mares …

Role Of Viral And Host Factors In Influenza Virus Mediated Inhibition Of Interleukin-23, Ashish Tiwari

Theses and Dissertations--Veterinary Science

Influenza virus is one of the major respiratory pathogens of humans as well as animals, including equines. There is an increasing evidence that bacterial infections are the most common cause of the death during influenza. In horses also, secondary bacterial pneumonia can lead to death, and surviving horses may take up to six months for the complete recovery resulting in heavy economic loss to the equine industry. Interleukin (IL)-23 mediated innate immune response has been shown to protect the host from various respiratory bacterial infections. However, studies to investigate the role of host and viral factors in the regulation of …

Studies On Equine Placentitis, Igor Frederico Canisso

Theses and Dissertations--Veterinary Science

Two types of placentitis were studied: ascending and nocardioform placentitis. Although the first diagnosis of nocardioform placentitis was made three decades ago, little is known about the disease, due to the lack of an experimental model. In attempt to develop a research model, Crossiela equi was inoculated through intrauterine, intravenous, intrapharyngeal, and oral routes, but none of the routes resulted in nocardioform placentitis. This may indicate that unidentified factors may play a role in disease pathogenesis and that simple presence of bacteria is not sufficient to induce nocardioform placentitis. The second and major component of this dissertation involved the identification …

The Role Of The Bacterial Endosymbiont, Arsenophonus, In The Soybean Aphid, Aphis Glycines, Jason A. Wulff

Theses and Dissertations--Entomology

Bacterial endosymbionts can have profound impacts on their host’s ecology. Notably, endosymbionts can protect their hosts against natural enemies and influence host plant interactions. The endosymbiont Candidatus Arsenophonus infects a wide taxonomic range of arthropod hosts, and is suspected of an uncharacterized mutualistic role in hemipterous insects. In the soybean aphid, Aphis glycines, an introduced pest of soybeans in the United States, Arsenophonus is the sole facultative endosymbiont. The focus of this dissertation is to characterize the role of Arsenophonus in the aphid, with an overall emphasis on its impact on aphid management strategies.

I first used diagnostic PCR …

Attitudes Toward Assisted Reproductive Technology: The Effects Of Gender, Relationship Status, Age, And Sexual Orientation, Brigitte A. Dooley

Theses and Dissertations--Family Sciences

Reproductive technology has extended procreative options to infertile, subfertile, unpartnered, and same-sex-partnered individuals, but this technology is sometimes used in circumstances that may be deemed unreasonable or inappropriate by some people. The purpose of this study was to assess the effects of five contextual variables—gender, relationship status, age, and sexual orientation of the individual or couple seeking reproductive assistance, as well as the source of gametes—on attitudes toward the procurement of reproductive services. A multiple-segment factorial vignette was administered to a sample of 257 reproductive-aged respondents. Results indicate that ART is generally viewed as an acceptable procedure by reproductive aged …

Changing Litter Resources Associated With Hemlock Woolly Adelgid Invasion Affect Benthic Communities In Headwater Streams, Christopher J. Strohm

Theses and Dissertations--Entomology

Hemlock woolly adelgid is an invasive herbivore causing extensive mortality of eastern hemlock, an important foundation species that provides stable conditions influencing biological communities. Hemlock is often found in riparian areas and following its decline, broadleaved species, including birch, beech, and rhododendron, will replace it. These plants differ from hemlock in patterns of canopy cover and leaf properties, which influence conditions and resources within streams.

My goal was to evaluate potential impacts of adelgid-induced alterations to riparian canopies and litter on benthic communities and litter breakdown in streams. I characterized benthic invertebrate communities, litter colonization and litter breakdown in streams …

Exploring The Links Between Seasonal Variation And Spider Foraging, Thomas Edward Dantas Whitney

Theses and Dissertations--Entomology

According to optimal foraging theory, generalist predators, such as spiders, are thought to feed indiscriminately on prey according to its availability, especially when food is scarce. In contrast, generalists can display selective feeding decisions under regimes of high prey abundance, but few studies have tracked changes in prey choice on a seasonal basis under open field conditions. Additionally, adaptations to surviving winter have been largely ignored in the research of foraging behavior. To elucidate this, I monitored prey availability and collected common forest-dwelling wolf spiders for molecular gut-content analysis, in parallel for 18 months, to assess the temporal changes occurring …

Assessing And Mitigating Lawn Insecticide Hazards To Bees And Other Beneficial Invertebrates, Jonathan Lane Larson

Theses and Dissertations--Entomology

Turfgrass settings, including lawns, golf courses, and sports fields, support many beneficial invertebrates that provide important ecosystem services. These non-target organisms and their associated predation, decomposition, and pollination services can be disrupted by the use of certain insecticides. I compared the ecotoxicity of representatives from three major turf insecticide groups, the neonicotinoids, premix formulations, and the anthranilic diamides, in lab and field realistic settings in order to inform industry initiatives towards environmental sustainability.

In lab and field bioassays clothianidin, a neonicotinoid, and a premix clothianidin/pyrethroid spray were acutely toxic to beneficial insects. Populations of predators, springtails, and earthworms, as well …

Graduates’ Perspectives On Transition From College Graduation To The Workplace: A Qualitative Approach, Katelyn B. Hendricks

Theses and Dissertations--Family Sciences

This article explored the experiences of college graduates in their program of study and during their transition from college graduation to the workplace. Factors focused on were perceptions of program and curriculum, employment preparation, connections to faculty and relationships with family members. Six individuals who graduated from the University of Kentucky Family Sciences (FAM) Department were interviewed. Participants openly shared positive and negative experiences. The study was examined through two theoretical lenses: Glen Elder’s life course perspective and Ludwig von Bertalanffy’s general systems theory. Participant interviews were transcribed verbatim and transcriptions were analyzed with inductive coding. Central themes that emerged …

Insecticide Resistance In The Bed Bug, Jennifer R. Gordon

Theses and Dissertations--Entomology

Populations of Cimex lectularius, the bed bug, have resurged around the world posing significant challenges for pest management professionals and causing physical, economic, and emotional strife. Pyrethroid resistance has been found in the vast majority of populations making pest management more difficult. The objectives of my dissertation research were to document the evolution of resistance to pyrethroid and neonicotinoid combination products (called combination products here) and to a neonicotinoid in the laboratory, to record potential fitness costs to resistance to the combination products, and to compare the efficacy of nine insecticides on six populations. In the laboratory, populations of …

Factors In Breastfeeding Initiation Among Central Kentucky Wic And Non-Wic Participants, Lila Nicole Farnsworth

Theses and Dissertations--Nutrition and Food Systems

Vital statistics data were statistically analyzed to determine who is breastfeeding in central Kentucky and if factors differ between those in a WIC and non-WIC population. The sample consisted of 479 postpartum women aged 18-44 in central Kentucky. Participants in the study were analyzed as a whole, then divided by their participation in the WIC program (n=304) or non-participation in the WIC program (n=175). Variables recorded in the vital statistics form were analyzed. These variables included age, education level, marital status, and ethnicity of the mother, household income status, and gender, gestational age, and birth weight of the infant. In …

Cortisol, Physical Activity, And Weight Loss In A Randomized Clinical Trial, Shawn S. Smith

Theses and Dissertations--Nutrition and Food Systems

Abnormal cortisol levels may be an important factor in the ability of an individual to lose weight and maintain weight loss. This study examined overweight and obese individuals who participated in a weight loss program. Cortisol, physical activity, and weight loss were measured at regular intervals and examined for possible relationships

Reconstruction Of The Hirnantian (Late Ordovician) Palaeotopography In The Upper Yangtze Region, Linna Zhang, Junxuan Fan, Qing Chen, Shuang-Ye Wu

Department of Earth, Environment and Sustainability Faculty Publications

Reconstruction of the Hirnantian (Late Ordovician) palaeotopography in South China is important for understanding the distribution pattern of the Hirnantian marine depositional environment. In this study, we reconstructed the Hirnantian palaeotopography in the Upper Yangtze region based on the rankings of the palaeo-water depths, which were inferred according to the lithofacies and biofacies characteristics of the sections. Data from 374 Hirnantian sections were collected and standardized through the online Geobiodiversity Database. The Ordinary Kriging interpolation method in the ArcGIS software was applied to create the continuous surface of the palaeo-water depths, i.e. the Hirnantian palaeotopography. Meanwhile, the line transect analysis …

"The Culture Of Leaks Has To Change", But At What Expense To Congressional Oversight Of The Executive Branch? An Examination Of Title V Of The Intelligence Authorization Act For Fiscal Year 2013., Roseanne Sessa

Student Works

No abstract provided.

Clinical Trials In Developing Countries, Michael William Jacobson

Student Works

No abstract provided.

Expert Witness, Anthony A. Graff

Student Works

No abstract provided.

The State As Trafficker: How Society Becomes Complicit In Child Sex Trafficking Through Its State Institutions, Anne M. Collart

Student Works

No abstract provided.

The Debate Over Regulation Alternatives For Cooling Water Intake Structures Is Heating Up, Nicole M. Magdziak

Student Works

No abstract provided.

Joseph Margolis, Aili W. Bresnahan

Philosophy Faculty Publications

Margolis’s methodology is best located in the pragmatic tradition, broadly construed. His pragmatism lies in his commitment to understanding the world as part of collective and consensual human practice and situated interaction; his embracing of the changing nature of history and science; and his approach to human knowledge as constructed.

In particular this pragmatic bent is evidenced by his affinity for Charles Sanders Peirce’s semeiotics, by which thought shows us the real world through the interpretation of signs and symbols, the existence of mind legitimated as “objective” and “real.” Margolis also uses Peirce’s theory of predicative generals (as constructed but …