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2014

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Articles 20401 - 20430 of 25853

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Deposition And Characterization Of Cu_9s_5 Nanocrystals From Unsymmetrical [(Hex)(Me)Nc(S)Nc(O)C_6h_3(No_2)_2-3,5]_2cu(Ii) And [(Et)(Bu)Nc(S)Nc(O)C_6h_4-4-No_2]_2cu(Ii) Complexes By Colloidal Thermolysis Method, Sohail Saeed, Rizwan Hussain Jan 2014

Deposition And Characterization Of Cu_9s_5 Nanocrystals From Unsymmetrical [(Hex)(Me)Nc(S)Nc(O)C_6h_3(No_2)_2-3,5]_2cu(Ii) And [(Et)(Bu)Nc(S)Nc(O)C_6h_4-4-No_2]_2cu(Ii) Complexes By Colloidal Thermolysis Method, Sohail Saeed, Rizwan Hussain

Turkish Journal of Chemistry

Cu_9S_5 nanocrystals were synthesized from unsymmetrical [(Hex)(Me)NC(S)NC(O)C_6H_3(NO_2)_2-3,5]_2Cu(II) and [(Et)(Bu)NC(S)NC(O)C_6H_4-4-NO_2]_2 Cu(II) complexes by colloidal thermolysis in the presence of surfactant oleylamine. The unsymmetrical copper complexes were synthesized by the reaction of copper(II) acetate with N-[hexyl(methyl)carbamothioyl]-3,5-dinitrobenzamide and N-[ethyl(butyl)carbamothioyl]-4-nitrobenzamide. The complexes were used as single-source precursors for the preparation of Cu_9S_5 nanocrsytals. The Cu_9S_5 nanocrystals were characterized by X-ray powder diffraction and transmission electron microscopy.

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Facile Synthesis Of 5-Bromotropono[C]-Fused Pyrazoles And Isoxazole, Yang Li, Liangyu Xu, Wentao Gao Jan 2014

Facile Synthesis Of 5-Bromotropono[C]-Fused Pyrazoles And Isoxazole, Yang Li, Liangyu Xu, Wentao Gao

Turkish Journal of Chemistry

A facile synthesis of a series of new 5-bromotropono[c]pyrazole derivatives (3 and 10--15) as well as 5-bromotropono[c]isoxazole (17) is described, involving a condensation reaction of 3-acetyl-5-bromotropolone (1) with hydrazine monohydrate (2), arylhydrazine hydrochlorides (4--9), and hydroxylamine hydrochloride (16), respectively. All the synthesized compounds were obtained in good yields of 56%--77% and their structures were characterized by spectral data and HRMS.

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Selective Oxidation Of Sulfides And Hydrocarbons With H_2o_2 Over Manganese Catalyst Supported On Nanoparticles, Massomeh Ghorbanloo, Roghayeh Tarasi, Jun Tao, Hidenori Yahiro Jan 2014

Selective Oxidation Of Sulfides And Hydrocarbons With H_2o_2 Over Manganese Catalyst Supported On Nanoparticles, Massomeh Ghorbanloo, Roghayeh Tarasi, Jun Tao, Hidenori Yahiro

Turkish Journal of Chemistry

A new magnetically separable catalyst consisting of binuclear Mn(II) complex [Mn_2(HL)_2(H_2O)_4], HL = 2-[(2-hydroxy-benzylidene)-amino]-3-(4-hydroxyphenyl)-propionic acid, supported on (3-chloropropyl)-trimethoxysilane (CPTMS) functionalized silica-coated magnetic nanoparticles (MNPs) was prepared. The synthesized catalyst was characterized by several physico-chemical and spectroscopic methods. This immobilized complex was found to be an efficient heterogeneous catalyst for the oxidation of different sulfides and hydrocarbons using hydrogen peroxide (H_2O_2) as an oxidant. The catalyst is readily recovered by simple magnetic decantation and can be recycled several times with no considerable loss of catalytic activity.

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Genotoxic Effects Of Zinc Oxide And Titanium Dioxide Nanoparticles On Root Meristem Cells Of Allium Cepa By Comet Assay, Eşref Demi̇r, Nuray Kaya, Bülent Kaya Jan 2014

Genotoxic Effects Of Zinc Oxide And Titanium Dioxide Nanoparticles On Root Meristem Cells Of Allium Cepa By Comet Assay, Eşref Demi̇r, Nuray Kaya, Bülent Kaya

Turkish Journal of Biology

Nanogenotoxicity is an emergent field, relevant for estimating the potential genotoxic risk of nanomaterials. In this study we investigated the genotoxic potential of zinc oxide (ZnO, Keywords: Zinc oxide nanoparticles, titanium dioxide nanoparticles, Allium cepa, comet assay, genotoxicity, DNA damage

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Development Of An Improved Rna Interference Vector System For Agrobacterium-Mediated Plant Transformation, Umut Toprak, Cathy Coutu, Doug Baldwin, Martin Erlandson, Dwayne Hegedus Jan 2014

Development Of An Improved Rna Interference Vector System For Agrobacterium-Mediated Plant Transformation, Umut Toprak, Cathy Coutu, Doug Baldwin, Martin Erlandson, Dwayne Hegedus

Turkish Journal of Biology

Plant-mediated RNA interference (RNAi) has shown a great potential in pest control and requires i) subcloning of sense/antisense regions in compatible vectors, ii) transfer of the silencing cassette into a binary vector, iii) transformation of Agrobacterium tumefaciens with desired binary plasmids, and iv) transformation of plants with Agrobacterium. The procedure is long and should ensure plasmid backbone stability; however, plasmid recombination due to antibiotic selection is a common problem. pGSA1252 is an RNAi silencing binary vector allowing direct cloning of hairpin structure; however, it possesses a chloramphenicol selection marker leading to plasmid recombination in various Agrobacterium strains. To solve this …

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The Genus Crocus, Series Crocus (Iridaceae) In Turkey And 2 East Aegean Islands: A Genetic Approach, Osman Erol, Hi̇lal Betül Kaya, Levent Şik, Meti̇n Tuna, Levent Can, Muhammed Bahatti̇n Tanyolaç Jan 2014

The Genus Crocus, Series Crocus (Iridaceae) In Turkey And 2 East Aegean Islands: A Genetic Approach, Osman Erol, Hi̇lal Betül Kaya, Levent Şik, Meti̇n Tuna, Levent Can, Muhammed Bahatti̇n Tanyolaç

Turkish Journal of Biology

In this study, a total of 26 Crocus specimens from different locations across Turkey and 2 East Aegean islands (Chios and Samos) were analyzed using 12 amplified fragment length polymorphism (AFLP) primer combinations to obtain information on genetic diversity, population structure, and genetic relationships. A total of 369 polymorphic AFLP bands were generated and scored as binary data. Genetic similarities were determined. Cluster analysis revealed 4 major groups among the 26 genotypes examined in this study. The nuclear DNA contents (2C) of the 26 Crocus specimens were found to range from 5.08 pg in C. asumaniae to 9.75 pg in …

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Molecular Characterization Of The Encoding Regions And Tissue Expression Analyses For 3 Novel Buffalo Akt Genes, Akt1, Akt2, And Akt3, Chunfeng Wu, Lixian Liu, Jinlong Huo, Dalin Li, Yueyun Yuan, Feng Yuan, Yongwang Miao Jan 2014

Molecular Characterization Of The Encoding Regions And Tissue Expression Analyses For 3 Novel Buffalo Akt Genes, Akt1, Akt2, And Akt3, Chunfeng Wu, Lixian Liu, Jinlong Huo, Dalin Li, Yueyun Yuan, Feng Yuan, Yongwang Miao

Turkish Journal of Biology

The objective of this study was to obtain the complete coding sequences (CDSs) of 3 buffalo genes (AKT1, AKT2, and AKT3) using reverse-transcriptase polymerase chain reaction and to depict their molecular characterizations and tissue expression patterns in buffalo. The buffalo AKT1, AKT2, and AKT3 CDSs were 1443 bp, 1446 bp, and 1440 bp in length and encoded 480, 481, and 479 amino acids, respectively. Nine, 13, and 3 nucleotide differences were found in the CDSs between buffalo and other bovine species. Phylogenetic analyses showed that buffalo AKT1 and AKT2 have close genetic relationships with other species in the family Bovidae, …

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Stimulatory Effect Of Methyl Jasmonate And Squalestatin On Phenolic Metabolism Through Induction Of Lox Activity In Cell Suspension Culture Of Yew, Zohreh Jalalpour, Leila Shabani, Ladan Afghani, Majid Sharifi-Tehrani, Sayed Asadollah Amini Jan 2014

Stimulatory Effect Of Methyl Jasmonate And Squalestatin On Phenolic Metabolism Through Induction Of Lox Activity In Cell Suspension Culture Of Yew, Zohreh Jalalpour, Leila Shabani, Ladan Afghani, Majid Sharifi-Tehrani, Sayed Asadollah Amini

Turkish Journal of Biology

Cell suspension cultures of Taxus baccata were treated with 2 elicitor compounds, methyl jasmonate (MeJA) and squalestatin (S), individually and in combination for 7 days to determine if they mediated the enhancement of biosynthesis of endogenous jasmonate through induction of lipoxygenase (LOX) activity. Total phenolic compounds, total flavonoids, total antioxidants, phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO), and LOX activities in 5-month-old yew cell cultures were studied. Our results showed that MeJA and S could stimulate production of phenol derivatives in cell suspension cultures of T. baccata. In parallel to the induction of phenolic production in elicited cells, results showed that …

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A Comparative Study On Plant Morphology, Gas Exchange Parameters, And Antioxidant Response Of Ocimum Basilicum L. And Origanum Vulgare L. Grown On Industrially Polluted Soil, Ira Stancheva, Maria Geneva, Yuliana Markovska, Nikolina Tzvetkova, Ivanka Mitova, Milka Todorova, Petar Petrov Jan 2014

A Comparative Study On Plant Morphology, Gas Exchange Parameters, And Antioxidant Response Of Ocimum Basilicum L. And Origanum Vulgare L. Grown On Industrially Polluted Soil, Ira Stancheva, Maria Geneva, Yuliana Markovska, Nikolina Tzvetkova, Ivanka Mitova, Milka Todorova, Petar Petrov

Turkish Journal of Biology

The effects of Cd, Pb, and Zn uptake on plant morphology, photosynthetic parameters, antioxidant potential, and essential oil yield and quality in Ocimum basilicum L. and Origanum vulgare L. plants were evaluated. The plants were grown as a pot experiment in soil heavily polluted with Cd and Pb and on unpolluted soil. Both plants accumulated Cd, mainly in the roots, while Pb occurred in the oregano shoots only. The leaf blade thickness of both plants increased when grown in polluted soil. Basil plants responded to the action of heavy metals with increases in gas exchange, stomatal conductivity, and transpiration, but …

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Population Dynamical Behaviors Of Stochastic Logistic System With Jumps, Ruihua Wu, Ke Wang Jan 2014

Population Dynamical Behaviors Of Stochastic Logistic System With Jumps, Ruihua Wu, Ke Wang

Turkish Journal of Mathematics

This paper is concerned with a stochastic logistic model driven by martingales with jumps. In the model, generalized noise and jump noise are taken into account. This model is new and more feasible. The explicit global positive solution of the system is presented, and then sufficient conditions for extinction and persistence are established. The critical value of extinction, nonpersistence in the mean, and weak persistence in the mean are obtained. The path-wise and moment properties are also investigated. Finally, some simulation figures are introduced to illustrate the main results.

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Defense Enzyme Activities And Biochemical Variations Of Pelargonium Zonale In Response To Nanosilver Application And Dark Storage, Mehrnaz Hatami, Mansour Ghorbanpour Jan 2014

Defense Enzyme Activities And Biochemical Variations Of Pelargonium Zonale In Response To Nanosilver Application And Dark Storage, Mehrnaz Hatami, Mansour Ghorbanpour

Turkish Journal of Biology

Pelargonium petal abscission and ethylene-induced leaf senescence are major problems that reduce the longevity of plants. This study was carried out to determine the poststorage influence of nanosilver (NS) particle (0, 20, 40, 60, and 80 mg L^{-1}) application during dark storage period on chlorophyll and carotenoid pigments, malondialdehyde (MDA) content, leaf protein and ethylene concentration, petal abscission, membrane stability index (MSI), and enzyme activity including ascorbate peroxidase (APX), peroxidase (POD), superoxide dismutase (SOD), catalase (CAT), and \BETA-glucosidase (\BETA-GLU) in two Pelargonium pot plants of the cultivars Flowerfairy and Foxi. Enhanced photosynthetic pigment contents were recorded in leaves of both …

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Comprehensive Analysis Of Beta-Galactosidase Protein In Plants Based On Arabidopsis Thaliana, Samin Seddigh, Maryam Darabi Jan 2014

Comprehensive Analysis Of Beta-Galactosidase Protein In Plants Based On Arabidopsis Thaliana, Samin Seddigh, Maryam Darabi

Turkish Journal of Biology

Beta-galactosidases (\BETAgals) (EC 3.2.1.23) have been detected in a wide range of plant organs and tissues and are described by their ability to hydrolyze terminal nonreducing \BETA-D-galactosyl residues from \BETA-D-galactosides. In this study, 92 \BETAgal protein sequences from different plants, 7 animal samples including human and mouse, 3 samples from bacteria including Escherichia coli, and 4 samples from insects including Drosophila melanogaster were aligned. Sequences were analyzed by computational tools to predict the protein properties, such as molecular mass, isoelectric point, signal peptide, motifs, transmembrane domain, and secondary and spatial structure. Protein structure analysis revealed there is a high identity …

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Cover And Contents Jan 2014

Cover And Contents

Turkish Journal of Biology

No abstract provided.

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Isolation And Amplification Of Genomic Dna From Barks Of Cinnamomum Spp., Valya Parambil Swetha, Viswanath Alambath Parvathy, Thotten Elampillay Sheeja, Bhaskaran Sasikumar Jan 2014

Isolation And Amplification Of Genomic Dna From Barks Of Cinnamomum Spp., Valya Parambil Swetha, Viswanath Alambath Parvathy, Thotten Elampillay Sheeja, Bhaskaran Sasikumar

Turkish Journal of Biology

Cinnamomum verum Presl (syn. C. zeylanicum Blume), the cinnamon of commerce, is an important aromatic tree spice having wide applications in perfumery, flavoring, beverages, and medicine. Adulteration of cinnamon with the cheaper and inferior barks of C. aromaticum and C. malabatrum is a problem. Morphological distinction of the barks is difficult; in the case of powdered barks, the situation is even worse. DNA-based molecular tools are preferred under these circumstances. Isolation of high quality DNA is a prerequisite for molecular studies, but barks contain polysaccharides, polyphenols, and secondary metabolites that hamper DNA isolation. Since attempts at isolating DNA using existing …

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Cover And Contents Jan 2014

Cover And Contents

Turkish Journal of Biology

No abstract provided.

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Improvement In Tolerance To Salt Stress During Tomato Cultivation, Mustafa Demi̇rkaya Jan 2014

Improvement In Tolerance To Salt Stress During Tomato Cultivation, Mustafa Demi̇rkaya

Turkish Journal of Biology

This study was conducted to evaluate the physiological impact of seed priming on improving the salt tolerance of tomato cultivars H-2274 and Rio Grande. Tomato seeds were primed with 5 M NaCl solution at 20 °C for 3 days in a dark environment. The seeds of the control treatments were handled in a similar way, using tap water instead of NaCl solution. After the priming procedure, seeds were washed and sown in standard germination trays. From each treatment group, 21 homogeneous seedlings were transplanted into 10-L pots full of turf for subsequent salinity experiments. In order to avoid osmotic shock, …

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Soil And Foliar Application Of Potassium Enhances Fruit Yield And Quality Of Tomato Under Salinity, Muhammad Amjad, Javaid Akhtar, Muhammad Anwar Ul Haq, Shakeel Imran, Sven-Erik Jacobsen Jan 2014

Soil And Foliar Application Of Potassium Enhances Fruit Yield And Quality Of Tomato Under Salinity, Muhammad Amjad, Javaid Akhtar, Muhammad Anwar Ul Haq, Shakeel Imran, Sven-Erik Jacobsen

Turkish Journal of Biology

Potassium plays a key role in the survival of plants under saline conditions in mitigating the adverse effects of sodium. The effect of application of potassium to soil (0, 3.3, and 6.6 mmol/kg) and leaves (4.5 and 9 mM) on tomato yield and quality under 3 salinity treatments (0, 7.5, and 15 dS m^{-1}), using 2 salt-tolerant (Indent-1 and Nagina) and 2 salt-sensitive (Peto-86 and Red Ball) genotypes, was studied in a pot experiment. Salinity decreased growth and yield of all genotypes; however, salt-tolerant genotypes maintained better growth and produced higher yield than the salt-sensitive genotypes across all salinity levels. …

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Purification And Characterization Of An Antibacterial Substance Produced By Pest-Originated Serratia Marcescens Mm3, Serpi̇l Uğraş, Kazim Sezen, Hati̇ce Kati, Zi̇hni̇ Demi̇rbağ Jan 2014

Purification And Characterization Of An Antibacterial Substance Produced By Pest-Originated Serratia Marcescens Mm3, Serpi̇l Uğraş, Kazim Sezen, Hati̇ce Kati, Zi̇hni̇ Demi̇rbağ

Turkish Journal of Biology

The aim of this investigation was to determine, purify, and characterize an antibacterial substance produced by Serratia marcescens Mm3 (Sm-Mm3) isolated from a common pest of hazelnut in Turkey, Melolontha melolontha, which is also a significant pest all over the world. A highly active antibacterial substance produced by Sm-Mm3 was purified by ammonium sulfate precipitation and high-performance liquid chromatography, respectively, and identified by spectrometric analysis (TOF-MS). Finally, we showed that this molecule is heat-stable, stable in the pH range of 5-9, and insensitive to organic solvents and proteinase K. The molecular mass was determined as 479 Da. Plasmid elimination demonstrated …

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Serum Leptin Profiles, Food Intake, And Body Weight In Melatonin-Implanted Syrian Hamsters (Mesocricetus Auratus) Exposed To Long And Short Photoperiods, Bülent Gündüz Jan 2014

Serum Leptin Profiles, Food Intake, And Body Weight In Melatonin-Implanted Syrian Hamsters (Mesocricetus Auratus) Exposed To Long And Short Photoperiods, Bülent Gündüz

Turkish Journal of Biology

We have shown that circadian changes in leptin concentrations are inversely linked to circulating melatonin levels in the Syrian hamster. The present study aimed to assess the effects of subcutaneous melatonin implants under different photoperiodic conditions: did the implants affect serum leptin levels, body weight, food consumption, and/or testicular weight? Male hamsters were exposed to long and short photoperiods for 10 weeks and received subcutaneous melatonin implants (1 mg in 24 mg beeswax) every 2 weeks. Blood samples were collected every week at midday (1200 hours) or midnight (0000 hours) to assess leptin and melatonin concentrations. Testes were measured to …

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Transgenic Nicotiana Tabacum Cultivar Samsun Plants Carrying The Wild Sugar Beet Hs1pro1 Gene Have Resistance To Root-Knot Nematodes, Çağla Sönmez, İbrahi̇m Hali̇l Elekci̇oğlu, Ayşe Meral Yücel, Hüseyi̇n Avni̇ Öktem Jan 2014

Transgenic Nicotiana Tabacum Cultivar Samsun Plants Carrying The Wild Sugar Beet Hs1pro1 Gene Have Resistance To Root-Knot Nematodes, Çağla Sönmez, İbrahi̇m Hali̇l Elekci̇oğlu, Ayşe Meral Yücel, Hüseyi̇n Avni̇ Öktem

Turkish Journal of Biology

Nematodes are the principal animal parasites of plants, causing annual crop losses of more than US$100 billion worldwide. Conventional control measures against nematode infection include toxic nematicide application to soil, crop rotation practices, and classical breeding approaches. However, due to the limitations of each technique, biotechnology presents itself as an effective alternative in nematode control. To date, several resistance genes against nematodes have been cloned. One such gene, Hs1^{pro1}, cloned from wild sugar beet, Beta procumbens, shows resistance to the cyst nematode Heterodera schachtii. Recent studies showed that Hs1^{pro1} has a broad target pathogen range. In this study, we transformed …

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The Modeled Structures Of Deg5 And Deg8 Proteases In Arabidopsis Thaliana, Liangbing Chen, Qingzhi Li, Lili Li Jan 2014

The Modeled Structures Of Deg5 And Deg8 Proteases In Arabidopsis Thaliana, Liangbing Chen, Qingzhi Li, Lili Li

Turkish Journal of Biology

Deg5 and Deg8 are chloroplastic proteases found in Arabidopsis thaliana. These proteases participate in the repair of photosystem II. However, little information exists on the structures of Deg5 and Deg8. Here, the Deg5 and Deg8 structures were modeled using the SWISS-MODEL tool with Deg1 as the structural template. Deg5 was composed of only 1 domain, a protease domain. The catalytic triad of Deg5, which comprises His147, Asp188, and Ser266, was located in the cleft between 2 \beta-barrels. Deg8 was composed of a protease domain at the N-terminal and a PDZ domain at the C-terminal. The catalytic triad of Deg8, which …

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Identification And Expression Profiling Of Clcuv-Responsive Transcripts In Upland Cotton (Gossypium Hirsutum L.), Beenish Aftab, Muhammad Naveed Shahid, Sania Riaz, Adil Jamal, Bahaeldeen Babiker Mohamed, Muzna Zahur, Mahwish Aftab, Bushra Rashid, Tayyab Husnain Jan 2014

Identification And Expression Profiling Of Clcuv-Responsive Transcripts In Upland Cotton (Gossypium Hirsutum L.), Beenish Aftab, Muhammad Naveed Shahid, Sania Riaz, Adil Jamal, Bahaeldeen Babiker Mohamed, Muzna Zahur, Mahwish Aftab, Bushra Rashid, Tayyab Husnain

Turkish Journal of Biology

CLCuV is one of the most serious causes of cotton damage for which no adequate remedy is available. The possible solution to this problem may be the transfer of resistant genes into the background of agronomical CLCuV-tolerant varieties. In response to viral infection, several plants may show qualitative and quantitative variations in gene expression. To study gene expression between diseased and tolerant plants, DDRT-PCR was performed. Upregulated gene expression was observed among cDNA transcripts screened through random primers. Seven transcripts have significant homology with known proteins (ribosomal protein, photosystem protein unit, membrane protein, and a predicted hypothetical protein). Quantitative real-time …

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Effect Of Water Deficiency On Seed Quality And Physiological Traits Of Different Safflower Genotypes, Hajar Amini, Ahmad Arzani, Mostafa Karami Jan 2014

Effect Of Water Deficiency On Seed Quality And Physiological Traits Of Different Safflower Genotypes, Hajar Amini, Ahmad Arzani, Mostafa Karami

Turkish Journal of Biology

Safflower is one of the most adapted oilseed crops to the drought-prone arid lands. This experiment was carried out to evaluate the effects of water deficiency on some physiological traits such as proline content, soluble carbohydrate content, relative water content (RWC), and seed quality using 64 safflower genotypes grown under normal and water-deficient field conditions. Plants were grown under normal irrigation until the branching growth stage, when water deficiency was applied to the plants. Results of combined analysis of variance showed the significant effects of genotype, water deficiency, and their interactions on the tested traits. RWC, seed oil content, and …

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A Cdna-Aflp Protocol With Reciprocally Arranged 2-Enzyme Sequential Digestion And Silver Staining Detection, Mehmet Ali̇ Südüpak Jan 2014

A Cdna-Aflp Protocol With Reciprocally Arranged 2-Enzyme Sequential Digestion And Silver Staining Detection, Mehmet Ali̇ Südüpak

Turkish Journal of Biology

This study provides a modified protocol to the standard cDNA-amplified fragment length polymorphism (AFLP) procedure that has been used to detect differentially expressed transcripts in various systems. Modifications aimed to improve the overall coverage of the technique and the isolation of single fragments from each of the cDNA species. The protocol utilizes oligo-d(T) coupled magnetic beads to isolate and synthesize, and 2-enzyme sequential digestions of ds-cDNA molecules with flip-flop strategy on the beads to release transcript tags representing individual mRNA type and quantity. The protocol has been applied to detect Puccinia sorghi-induced expressional changes in maize leaf material sampled at …

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Photocatalytic Antimicrobial Effect Of Tio_2 Anatase Thin-Film-Coated Orthodontic Arch Wires On 3 Oral Pathogens, Fi̇gen Özyildiz, Ataç Uzel, Ayşe Serpi̇l Hazar, Mustafa Güden, Sultan Ölmez, Işil Aras, İsmai̇l Karaboz Jan 2014

Photocatalytic Antimicrobial Effect Of Tio_2 Anatase Thin-Film-Coated Orthodontic Arch Wires On 3 Oral Pathogens, Fi̇gen Özyildiz, Ataç Uzel, Ayşe Serpi̇l Hazar, Mustafa Güden, Sultan Ölmez, Işil Aras, İsmai̇l Karaboz

Turkish Journal of Biology

The aim of this study was to introduce antimicrobial activity to stainless steel orthodontic arch wires by coating them with TiO_2 in anatase form. Stainless steel (0.016 × 0.022 inch), D-rect (0.016 × 0.022 inch), and multistranded hammered retainer wires (0.014 × 0.018 inch) were coated with TiO_2 anatase by the sol-gel dip coating method. The wires were assessed for their photocatalytic antimicrobial activity against Streptococcus mutans, Candida albicans, and Enterococcus faecalis. After illumination under UVA (315-400 nm) at 1.0 mW/cm^2 for 1 h, the reduction efficiencies of the anatase-coated arch wires were calculated by using colony-forming unit counts. All …

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Cover And Contents Jan 2014

Cover And Contents

Turkish Journal of Biology

No abstract provided.

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Production Of Biosurfactant By Pseudomonas Spp. Isolated From Industrial Waste In Turkey, Tayfun Kaya, Belma Aslim, Ergi̇n Kari̇ptaş Jan 2014

Production Of Biosurfactant By Pseudomonas Spp. Isolated From Industrial Waste In Turkey, Tayfun Kaya, Belma Aslim, Ergi̇n Kari̇ptaş

Turkish Journal of Biology

In this study, 26 Pseudomonas spp. were isolated from a stream polluted by factory waste and from petroleum-contaminated soil. The surface tension (ST) of the cultures was used as a criterion for the primary isolation of biosurfactant-producing bacteria. Biosurfactant production was quantified by ST reduction, critical micelle concentration (CMC), emulsification capacity (EC), and cell surface hydrophobicity (CSH). Two of the isolates, P. aeruginosa 78 and 99, produced rhamnolipid biosurfactant. The strains started rhamnolipid production in the logarithmic phase. They decreased the ST of the culture from 73 dyne/cm^2 to 29 and 33 dyne/cm^2, and the CMC of produced rhamnolipids were …

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Activity Of Antioxidant Enzymes During Induction Of Morphogenesis Of Fritillaria Meleagris In Bulb Scale Culture, Marija Petric, Sladana Jevremovic, Milana Trifunovic, Vojin Tadic, Snezana Milosevic, Angelina Subotic Jan 2014

Activity Of Antioxidant Enzymes During Induction Of Morphogenesis Of Fritillaria Meleagris In Bulb Scale Culture, Marija Petric, Sladana Jevremovic, Milana Trifunovic, Vojin Tadic, Snezana Milosevic, Angelina Subotic

Turkish Journal of Biology

We investigated the activity of 3 antioxidant enzymes: superoxide dismutase, catalase, and peroxidase during morphogenesis of Fritillaria meleagris L. in vitro in bulb scale culture. Bulb cultures of F. meleagris were established on nutrition medium supplemented with 1.0 mg L^{-1} thidiazuron. Bulbs were grown at standard (24 °C) and low temperatures (4 °C) or at an increased sucrose concentration (4.5%) during the first 6 weeks (pretreatments). All bulbs were then grown for 4 weeks at standard temperature on 2, 4-dichlorophenoxyacetic acid and kinetin or thidiazuron (at concentrations of 1 mg L^{-1} each) in order to examine the influence of the …

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Expression And Purification Of Porcine Akirin2 In Escherichia Coli, Xiaoling Chen, Zhiqing Huang, Bo Zhou, Huan Wang, Gang Jia, Jiayun Qiao Jan 2014

Expression And Purification Of Porcine Akirin2 In Escherichia Coli, Xiaoling Chen, Zhiqing Huang, Bo Zhou, Huan Wang, Gang Jia, Jiayun Qiao

Turkish Journal of Biology

Akirin2 is a recently discovered gene related to immune responses that also plays an important role in skeletal myogenesis. In this study, in order to scale up the production of recombinant porcine Akirin2 (pAkirin2), we report the expression and purification of a His-tagged version of recombinant pAkirin2 in Escherichia coli. The pAkirin2 is a polypeptide of 203 amino acids containing 42 rare codons. The recombinant pAkirin2 was expressed as an N-terminal fusion protein with His6 tag in E. coli Rosetta (DE3) host strain. The protein was purified by Ni-IDA affinity chromatography, yielding over 90% highly purified recombinant pAkirin2, with a …

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Calcitriol Modulates The Effects Of The Supernatants Of Bone-Marrow-Derived Mesenchymal Stem Cells On Neutrophil Functions, Hadi Esmaili Gouvarchin Galeh, Norouz Delirezh, Seyyed Meysam Abtahi Froushani, Nahideh Afzale Ahangaran Jan 2014

Calcitriol Modulates The Effects Of The Supernatants Of Bone-Marrow-Derived Mesenchymal Stem Cells On Neutrophil Functions, Hadi Esmaili Gouvarchin Galeh, Norouz Delirezh, Seyyed Meysam Abtahi Froushani, Nahideh Afzale Ahangaran

Turkish Journal of Biology

Mesenchymal stem cells (MSCs) in bone marrow form a niche that has inevitable interactions with neutrophils. Moreover, previous documents have shown that calcitriol has an important role in regulating the cell growth of MSCs. This study set out to investigate the effects of calcitriol on the interaction between bone-marrow-derived MSCs and neutrophils. MSCs were isolated from the bone marrow of rats and pulsed with different concentrations of calcitriol (50, 100, and 200 nM) for different periods of time (24, 48, and 72 h). As the next step, the supernatants of MSCs cocultured with neutrophils for 4 h and neutrophil functions …

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