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Role Of Microrna Let-7 In Pancreatic Beta Cells, Shungang Zhang

Dissertations, Master's Theses and Master's Reports - Open

MicroRNAs (miRNAs) are small non-coding RNAs that inhibit gene expression at transcriptional or post-transcriptional level. Let-7 family is among the first identified human miRNAs and regulates multiple cellular processes including glucose metabolism in multiple organs. It has been reported that overexpression of let-7 resulted in insulin resistance and impaired glucose tolerance through repressing insulin signaling pathway in both muscle and liver. However, the role and mechanism underlying let-7 function in pancreatic beta-cells have yet to be elucidated.

Let-7 family contains nine members, which poses a significant challenge in complete deletion of this miRNA family. To study the function of let-7 …

Certified Personal Trainers And Physicians: An Evaluation Of Consumer Preference, Influence, And Decision Making On Intent To Use A Weight-Loss Medication, Linda Lemgruber Lobb

Electronic Theses and Dissertations

In health-related behavioral decision-making, the Theory of Planned Behavior (TPB) posits that attitudes toward a behavior are the function of three metrics: beliefs and strengths of beliefs toward the behavior, subjective norms around the behavior and locus of control for the individual (Ajzen, 1988). The purpose of this research was to identify the extent to which consumers would accept the recommendation of taking weight-loss medications, based on the source of the recommendation. Research shows that the source of knowledge for information plays a significant role in health-related decision-making of consumers (Pornpitakpan, 2004). In comparison to traditional weight-loss methods such as …

Parent's Use And Likely Use Of Nutrition Education Resources In The Mississippi Delta Region, Rachel Jessica Scoggins

Electronic Theses and Dissertations

Objective: To identify nutrition education resources currently being used and those likely to be used if made available by parents of elementary-aged children in the Mississippi Delta region. Methods: Surveys were completed by 214 parents (92% female, 88% African American) of children in grades K-2nd from three schools in the Mississippi Delta region. Survey items obtained nutrition education resources currently used by parents , those likely to be used if made available, mode of delivery and mediators (individuals) providing nutrition education. Results: Parents reported high importance that their child eats healthy. Physicians were identified as the primary mediator for delivering …

The Role Of Religion In The Relationship Between Loneliness And Health Behaviors, Lindsy Jean Magee

Electronic Theses and Dissertations

While research has demonstrated the relationship of religion to health risk and health promoting behaviors, it has not investigated the relationship between religious coping and health risk and health promoting behaviors. In addition, while the link between loneliness and certain health risk and health promoting behaviors has been established, little research has looked at the potential effect that religion has on the relationship between loneliness, health risk, and health promoting behaviors. As such, it was the purpose of this study to investigate the relationship between religious coping and health risk and health promoting behaviors and to investigate the role of …

Massachusetts Healthy Aging Data Report: Community Profiles, Elizabeth Dugan, Frank Porell, Nina Silverstein, Ruth Palombo, Stacey Mann

Gerontology Institute Publications

The Massachusetts Healthy Aging Data Report: Community Profiles was created by researchers at the Gerontology Institute of the John W. McCormack Graduate School of Policy and Global Studies at the University of Massachusetts Boston and commissioned by the Tufts Health Plan Foundation.

In this report, we have created a custom profile of nearly 100 healthy aging indicators for every city and town in Massachusetts including the 16 neighborhoods of Boston (367 Community Profiles). Each Community Profile is designed to help community residents, agencies, providers, and governments understand the older adults who live in their cities and towns – their ages, …

The Effectiveness Of Various Reminder Systems On Appointment-Keeping Adherence In A University-Based Primary-Care Setting, Frank M. Mirarchi

PCOM Psychology Dissertations

Treatment nonadherence is a significant problem that can affect not only the healthcare industry, but also more importantly, a patient’s health. Appointment-keeping adherence is expected to supersede all other types of adherence in terms of its importance and sequence. Various interventions have been used to treat nonadherence including appointment-keeping nonadherence within medical practices. Some of these interventions include different types of reminders (phone, letter, cards, etc.) and different types of therapy (motivational interviewing (MI) and cognitive behavioral therapy). Both reminders and MI have been effective in the past in treating appointment-keeping nonadherence. Therefore, will an MI-formatted letter reminder improve appointment-keeping …

In Vitro Cytotoxic, Genotoxic, And Oxidative Effects Of Acyclic Sesquiterpene Farnesene, Kübra Çeli̇k, Başak Toğar, Hasan Türkez, Numan Taşpinar

Turkish Journal of Biology

Farnesene (FNS) is an acyclic sesquiterpene. It has a wide range of important biological effects such as antioxidant, antimicrobial, and antifungal properties, although its cytotoxic, cytogenetic, and oxidative effects have not been investigated in human blood tissue yet. To this aim, both MTT and lactate dehydrogenase (LDH) assays were carried out to evaluate cell viability and cytotoxicity. Total antioxidant capacity (TAC) and total oxidative stress (TOS) parameters were used to assess oxidative alterations. In addition, micronucleus and chromosomal aberration tests were used for mutagenic and genotoxic studies. The results revealed that FNS reduced cell viability at concentrations of higher than …

Genome-Wide Identification And Analysis Of Growth Regulating Factor Genes In Brachypodium Distachyon: In Silico Approaches, Ertuğrul Fi̇li̇z, İbrahi̇m Koç, Hüseyi̇n Tombuloğlu

Turkish Journal of Biology

Growth-regulating factor (GRF) genes may play important roles for regulating growth and development in different plant tissues and organs. Here we report the first genome-wide analysis of the GRF gene family in Brachypodium. We performed in silico comparative analysis of GRF genes, including their structure, duplication in the genome, conserved motifs, and phylogenetic relationship. At the end of the study, 10 BdGRF genes were identified. The highest number of GRF genes was identified on chromosome 1 with 5 members, whereas the least number of genes (only 1 member) was found on chromosomes 2, 4, and 5. Of those, a single …

Investigation Of Potential Virulence Genes And Antibiotic Resistance Characteristics Of Enterococcus Faecalis Isolates From Human Milk And Colostrum Samples, Si̇ne Özmen Toğay, Ayhan Temi̇z, Ayten Çelebi̇, Leyla Açik, Siddi̇ka Songül Yalçin

Turkish Journal of Biology

Enterococci may improve the typical taste and flavor of fermented foods through their proteolytic and lipolytic activities. However, some enterococcal strains are recognized as nosocomial pathogens, which have virulence genes and resistance to certain antibiotics. Enteroccocci are also found in human milk microflora. The aim of this study was to investigate the potential virulence genes and antibiotic resistance characteristics of Enterococcus faecalis isolates from human milk and colostrum samples. In total, 23 Enterococcus faecalis strains were identified from human milk and colostrum samples. Antibiotic-resistant E. faecalis isolates were determined using the disk diffusion method. Vancomycin resistance genes (vanA, vanB) and …

Prothoracicotropic Hormone-Producing Neurosecretory Neurons And Antioxidative Defense In Midgut Of Lymantria Dispar In Trophic Stress, Vesna Peric Mataruga, Milena Vlahovic, Marija Mrdakovic, Dajana Todorovic, Dragana Matic, Anja Gavrilovic, Larisa Ilijin

Turkish Journal of Biology

As a very invasive insect species, Lymantria dispar is adaptable and sensitive to a changing environment. In insects the neuroendocrine system first reacts to stress by production of prothoracicotropic neurohormones (PTTH) that control ecdysteroid synthesis (morphogenetic and stress hormones). In this article, we report changes in the L2' brain neurosecretory neurons that synthesize PTTH in L. dispar larvae after feeding on locust tree leaves (Robinia pseudoacacia), an unsuitable host plant. Groups of larvae (n = 20 per experimental group) were offered this in comparison with oak leaves (Quercus robur), a suitable control diet, for 3 days after molting into the …

Herbicide-Tolerant Sugarcane (Saccharum Officinarum L.) Plants: An Unconventional Method Of Weed Removal, Idrees Ahmad Nasir, Bushra Tabassum, Zahida Qamar, Muhammad Aslam Javed, Muhammad Tariq, Abdul Munim Farooq, Shahid Javed Butt, Abdul Qayyum, Tayyab Husnain

Turkish Journal of Biology

Unnecessary weed growth in sugarcane fields forces plants to compete for nutrients and sunlight for survival, which most often leads to significant yield losses. As chemical herbicides cannot differentiate between crop plants and weeds, the development of herbicide-tolerant crops is anticipated. Four sugarcane varieties, CPF-234, CPF-213, HSF-240, and CPF-246, were used to develop glyphosate herbicide tolerance. A glyphosate-tolerant gene of 1368 bp cloned directionally under the 35S promoter with the \Beta-glucuronidase (GUS) reporter gene was used as the transgene. Through the biolistic transformation of sugarcane, calli of all cultivars were transformed with glyphosate-tolerant gene constructs. Efficient regeneration conditions were optimized …

Expression And Purification Of Porcine Pid1 Gene In Escherichia Coli, Huan Wang, Xiaoling Chen, Zhiqing Huang, Bo Zhou, Gang Jia, Guangmang Liu, Hua Zhao

Turkish Journal of Biology

In this study, in order to scale up the production of recombinant porcine phosphotyrosine interaction domain containing 1 (pPID1), a pET-28a (+)-pPID1 expression plasmid was constructed and transformed into Escherichia coli Rosetta (DE3). The recombinant pPID1 was then purified and identified by western blotting, and was also analyzed in vitro for its function. The recombinant protein was tagged with only a His6 tag at its C-terminus, which could be conveniently purified by affinity column. The protein could be induced for efficient expression with 0.75 mM IPTG for 8 h at 30 °C, yielding approximately 3 mg/L. In vitro biological activity …

Evaluation Of Orange Peel, An Industrial Waste, For The Production Of Aspergillus Sojae Polygalacturonase Considering Both Morphology And Rheology Effects, Ni̇han Göğüş, Bengi̇ Hakgüder Taze, Hande Demi̇r, Canan Tari, Sevcan Ünlütürk, Marcelo Fernandez Lahore

Turkish Journal of Biology

Orange peel is an agroindustrial waste rich in pectin and known to be an inducer for pectinase production. The use of this low-cost substrate for the production of an industrially important enzyme, polygalacturonase (PG), can be an alternative way to turn this waste into a value-added product, contributing to the reduction of environmental waste disposal problems. Enzyme productions by fungal microorganisms are affected by environmental and nutritional factors, demanding the determination of optimum conditions for maximum enzyme production with the desired fungal morphology and broth rheology. Therefore, complex and additional carbon sources were optimized with respect to PG production by …

Impact Of Salt Stress On Photosystem Ii Efficiency And Antioxidant Enzyme Activities Of Safflower (Carthamus Tinctorius L.) Cultivars, Şeküre Çulha Erdal, Hüsnü Çakirlar

Turkish Journal of Biology

This study was conducted to determine the tolerance of safflower (Carthamus tinctorius L.) cultivars (Dinçer, Remzibey-05, and Yenice) against salt stress based on some physiological and biochemical parameters at the vegetative stage. Eighteen-day-old plants were subjected to salt stress [0 (control), 75, 150, 225, and 300 mM NaCl concentrations] for 12 days, which led to a significant decrease in growth parameters (stem growth, fresh weight, and relative water content) and the photosynthetic pigment contents of the safflower cultivars. The photochemical activities of photosystem II of the cultivars were negatively affected by salinity, especially at the highest concentration (300 mM). Salt …

Phylogenetic Analysis And Characterization Of An Alkane-Degrading Yeast Strain Isolated From Oil-Polluted Soil, Ortansa Csutak, Tatiana Vassu

Turkish Journal of Biology

The isolation and phylogenetic analysis of new yeast strains represent an important basis for the development of practical applications in biotechnology, industry, and bioremediation. The yeast strain RG1 was isolated from oil-polluted soil and characterized using morphological observations and the API 20C AUX system. The amplified ITS1-5.8S rDNA-ITS2 region had 653 bp and the restriction profiles obtained with Cfo I, Dde I, Hae III, Hinf I, and Hpa II endonucleases showed high similarities with those from Rhodotorula glutinis reference strains. The phylogenetic tree based on the Cfo I patterns confirmed the classification of the strain RG1 as belonging to the …

Cancer Chemopreventive Effect Of Dietary Zataria Multiflora Essential Oils, Abolfazl Dadkhah, Faezaeh Fatemi, Mohammad Reza Mohammadi Malayeri, Azadeh Rasooli

Turkish Journal of Biology

Zataria multiflora Boiss., with the common name Avishan-e-Shirazi, is native to Iran. This herb has been found to possess varied pharmacological properties. In the present study, for the first time, colon chemopreventive effects of Z. multiflora essential oils (0.01% and 0.1% in the diet) in rats treated with 1,2-dimethylhydrazine (DMH) were demonstrated. For this purpose, the oxidative stress/antioxidant parameters (lipid peroxidation, glutathione, superoxide dismutase, catalase, and ferric reducing ability of plasma) concomitant with xenobiotic metabolizing enzymes (CYP450 and GST) were considered. Moreover, the colonic Beta-catenin protein was examined in colon tissues followed by histopathological analysis. The results showed that the …

Concurrent Validity Of Accelerations Measured Using A Tri-Axial Inertial Measurement Unit While Walking On Firm, Compliant, And Uneven Surfaces, Michael H. Cole, Wolbert Van Den Hoorn, Justin K. Kavanagh, Steven Morrison, Paul W. Hodges, James E. Smeathers, Graham K. Kerr

Rehabilitation Sciences Faculty Publications

Although accelerometers are extensively used for assessing gait, limited research has evaluated the concurrent validity of these devices on less predictable walking surfaces or the comparability of different methods used for gravitational acceleration compensation. This study evaluated the concurrent validity of trunk accelerations derived from a tri-axial inertial measurement unit while walking on firm, compliant and uneven surfaces and contrasted two methods used to remove gravitational accelerations; i) subtraction of the best linear fit from the data (detrending); and ii) use of orientation information (quaternions) from the inertial measurement unit. Twelve older and twelve younger adults walked at their preferred …

The Effects Of A Cooperative Learning Environment On Preservice Elementary Teachers' Interest In And The Application Of Music Into Core Academic Subjects, John Okley Egger

Theses and Dissertations--Music

The purpose of this study was to examine the effect of cooperative learning on preservice elementary teachers’ interest in, and the application of music into, core academic subject lesson plans. Participants (N = 59) were preservice elementary teachers enrolled in four class sections of a music method course designed for elementary education majors at a large southern university. All members participating in the study were placed by section for eight weeks in one of two groups-an individualistic learning group or cooperative learning group.

During the first 6 weeks of the study, participants worked on the Music Integration Project. …

Stress Fractures In An Athlete With Primary Amenorrhea After Onset Of Menarche At Age Twenty-Two: A Case Report, Jill Inouye, Ann Gately, Christopher Mcgrew

UNM Orthopaedic Research Journal

No abstract provided.

The Environment Of Poverty And The Limits Of Girlhood: The Struggle For The Right To An Education In North India. Gareebi Ka Mahual Aur Ladkeo Ki Hud, Imandeep Kaur Grewal

Master's Theses and Doctoral Dissertations

This qualitative dissertation explored the lived worlds and educational experiences of adolescent girls living in poverty in North India. Class, caste, gender, and regionally-based inequalities result in striking disparities leading to restricted, gendered opportunities and individual freedoms. The purpose of this ethnographic study was an in-depth exploration of the lives of 20 girls living in poverty, and how their own educational futures were impacted by educational policy and practices. Illustrative case studies were used to explore the broader question of educational access. In keeping with Article 12 of the Convention on the Rights of the Child (CRC), the primary focus …

Dysmenorrhea And Body Mass Index, Marisa Richard

Master's Theses and Doctoral Dissertations

Dysmenorrhea is a common gynecological complaint among women, one that costs the U.S. economy approximately $2 billion in lost productivity each year. The present observational study examined the correlation of BMI and diagnoses of dysmenorrhea in a large sample of women ages 18-40 seen in an outpatient setting over the course of one year at a large health system in southeast Michigan. Although there were no statistically significant differences in the probability of dysmenorrhea among women of different BMI groups, the trending information seemed to suggest that dysmenorrhea is not influenced by BMI categories per se, but by extremes in …

The Choice Is Yours! Researchers Assign Subject Metadata To Their Own Materials In Institutional Repositories, Maira Bundza

University Libraries Faculty & Staff Publications

The Digital Commons platform for institutional repositories provides a three-tiered taxonomy of academic disciplines for each item submitted to the repository. Since faculty and departmental administrators across campuses are encouraged to submit materials to the institutional repository themselves, they must also assign disciplines or subject categories for their own work. The expandable drop-down menu of about 1,000 categories is easy to use, and facilitates the growth of the institutional repository and access to the materials through the Internet.

Environmental Factors Influencing Drinking Water Consumption And Access In Rural Communities: Cuba, New Mexico, Courtney Thornton, Theresa Cruz, Sally Davis

Posters and Presentations

Consumption of sugar sweetened beverages (SSBs) contributes to increasing obesity rates because they are a highly caloric beverage with poor nutritional value. Substituting SSB consumption with water consumption will help to decrease obesity. Water consumption is affected by various factors, including water access, a clean water supply, SSB popularity, policies, recommendations, and individual perceptions. Little research has addressed water consumption in rural populations. We are investigating access to drinking water of residents of the rural multicultural town of Cuba, NM. A literature review is being conducted on the advantages of water consumption and adverse effects of SSBs. Field observations assessed …

Can Home Gardens Scale Up Into Movements For Social Change? The Role Of Home Gardens In Providing Food Security And Community Change In San Jose, California, Ann Drevno, Additional Author(S)

Staff Works

No abstract provided.

Trained Immunity Or Tolerance: Opposing Functional Programs Induced In Human Monocytes After Engagement Of Various Pattern Recognition Receptors, Daniela C. Ifrim, Jessica Quintin, Leo A.B. Joosten, Cor Jacobs, Trees Jansen, Liesbeth Jacobs, Neil A.R. Gow, David L. Williams, Jos W.M. Van Der Meer, Mihai G. Netea

ETSU Faculty Works

Upon priming with Candida albicans or with the fungal cell wall component β-glucan, monocytes respond with an increased cytokine production upon restimulation, a phenomenon termed "trained immunity." In contrast, the prestimulation of monocytes with lipopolysaccharide has long been known to induce tolerance. Because the vast majority of commensal microorganisms belong to bacterial or viral phyla, we sought to systematically investigate the functional reprogramming of monocytes induced by the stimulation of pattern recognition receptors (PRRs) with various bacterial or viral ligands. Monocytes were functionally programmed for either enhanced (training) or decreased (tolerance) cytokine production, depending on the type and concentration of …

The Effect Of Direct Payments To Rice Farming Households In Korea, Jaehyung Kim

MPA/MPP/MPFM Capstone Projects

1) Background

Rice farming is a very important agricultural policy issue for the Korean government not only because rice is a staple food for the Korean population but because rice production is also an important source of income for those involved in rice farming. The importance of rice production to the agricultural sector of Korea is indicated by the fact that 49% of Korea’s agricultural land is devoted to rice production. Therefore, Korean agricultural policy has focused on rice production issues as well as the income of farmers involved in rice production.

To support rice farming income, the Korean government …

The Patient Protection And Affordable Care Act’S Effects On Patterns Of Coverage In A Ryan White Funded Hiv Clinic: What Are The Implications For The Bluegrass Care Clinic?, Jacqueline Wagner Mccuddy

MPA/MPP/MPFM Capstone Projects

The Ryan White CARE Act of 1991 implemented a federal grant program to help individuals in the US with HIV/AIDS get access to healthcare and diminish barriers to care. Since its enactment, there have been many changes in the environment of HIV/AIDS, including a demographic shift in those most affected by the disease and a growing number of new infections, the introduction of Antiretroviral therapy, and most recently, the implementation of the Patient Protection and Affordable Care Act (PPACA).

The PPACA is expected to have a positive effect on patients with HIV in the US by increasing access to care, …

Assessment Of The Quality Of Public Data Reporting By Nonprofit Social Service Agencies Receiving Funding From The Lexington-Fayette Urban County Government In Fy 2013, Yuanyuan Song

MPA/MPP/MPFM Capstone Projects

In 2012 and 2013, Lexington Fayette Urban County Government (LFUCG) Department of Social Service requested a “Social Service Needs Assessment Project” in Fayette County, Kentucky. In Aug, 31, 2012, Social Service Department established the “Needs Assessment Budget”. During Mar-Oct, 2013, two teams from The University of Kentucky College of Social Work (COSW) and the Martin School of Public Policy and Public Administration worked together to accomplish this project.

One of the tasks of this project is to assess the quality of data reported by the social service partner agencies of Lexington. “Partner organization” is the name government officials give to …

The Impact Of Sea Surface Temperature On Outbreaks Of Acanthaster Planci On The Great Barrier Reef, Laura A. Grossman

Scripps Senior Theses

The causes of increasing outbreaks of Acanthaster planci on the Great Barrier Reef have been a point of hot debate in recent years. It is unknown whether the increased success is due to nutrient runoff, salinity levels, or a decrease in predation, among other possibilities. In this paper I argue that the primary influence on outbreak status is sea surface temperature. From existing literature, I demonstrate that sea surface temperature in the Great Barrier Reef has increased by 0.4°C per year over the past three decades. I attempt to tie this increase with an increase in frequency of A. planci …

Bayesian Pathway Analysis Of Cancer Microarray Data, Melike Korucuoglu, Senol Isci, Arzucan Ozgur, Hasan H. Otu

Department of Electrical and Computer Engineering: Faculty Publications

High Throughput Biological Data (HTBD) requires detailed analysis methods and from a life science perspective, these analysis results make most sense when interpreted within the context of biological pathways. Bayesian Networks (BNs) capture both linear and nonlinear interactions and handle stochastic events in a probabilistic framework accounting for noise making them viable candidates for HTBD analysis. We have recently proposed an approach, called Bayesian Pathway Analysis (BPA), for analyzing HTBD using BNs in which known biological pathways are modeled as BNs and pathways that best explain the given HTBD are found. BPA uses the fold change information to obtain an …