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Novel Serum Biomarkers For Lung Cancer Early Diagnosis And Clinical Outcome, Fanmao Zhang

Dissertations and Theses (Open Access)

The five-year survival rate for all stages of lung cancer combined is only 17%, which has changed little over the past 40 years. Despite the tremendous efforts made, serum biomarkers with clinical utility for lung cancer early detection and clinical outcome prediction are still lacking. Metabolic alterations have been recognized as an emerging hallmark of cancer. We aimed to investigate the metabolic changes associated with lung cancer and to identify novel clinically applicable serum biomarkers for lung cancer early diagnosis and clinical outcome.

Serum metabolites are potential biomarkers for lung cancer early detection. We first performed global metabolomic profiling followed …

Genetic Predictors Of Metabolic Side Effects Of Diuretic Therapy, Jorge L. Del Aguila

Dissertations and Theses (Open Access)

Thiazide diuretics are a recommended first-line monotherapy for hypertension (i.e.SBP>140 mmHg or DBP>90 mmHg). Even so, diuretics are associated with adverse metabolic side effects, such as hyperlipidemia, hyperglycemia and hypokalemia which increase the risk of developing type II diabetes. This thesis used three analytical strategies to identify and quantify genetic factors that contribute to the development of adverse metabolic effects due to thiazide diuretic treatment. I performed a genome-wide association study (GWAS) and meta-analysis of the change in fasting plasma glucose and triglycerides in response to HCTZ from two different clinical trials: the Pharmacogenomic Evaluation of Antihypertensive Responses …

Dissecting The Roles Of Trim24 In Regulation Of Hepatic Lipid Metabolism And Inflammation, Lindsey C. Minter

Dissertations and Theses (Open Access)

DISSECTING THE ROLES OF TRIM24 IN REGULATION OF HEPATIC LIPID

METABOLISM AND INFLAMMATION

Lindsey Cauthen Minter, B.S., B.A.

Advisory Professor: Michelle C. Barton, Ph.D.

In this dissertation, I report the characterization of a new mouse model that recapitulates development of hepatocellular carcinoma (HCC) following spontaneous hepatic lipid accumulation, inflammation, and damage of liver tissue, due to complete loss of Trim24 expression. In human HCC and other cancers, TRIM24 expression is aberrantly high, while deletion of TRIM24 in the mouse has been shown to act as a liver specific tumor suppressor. The hypothesis tested here was that TRIM24, the E3 ubiquitin …

Angiomotin Is A Novel Cadherin-11 Interacting Protein That Mediates Migration In Prostate Cancer Cells, Angelica Ortiz

Dissertations and Theses (Open Access)

Prostate cancer (PCa), the second leading cause of cancer-related deaths among men in the United States, has the proclivity to metastasize to bone resulting in sclerotic lesions. These cancer induced bone growths cause bone pain and fractures. Therefore, understanding the molecular mechanisms contributing to PCa bone metastasis is required in order to find better prognostic tools and suitable targets for metastasis treatment and/ or prevention. Previous work in our laboratory showed increased expression of cadherin-11 (Cad11), a mesenchymal cadherin, during PCa progression. Furthermore, Cad11 expression endows PCa cells with increased migratory potential and metastasis to bone. Deletion of the Cad11 …

Energy Stress Causes Chaperones To Assemble Into Cytoplasmic Complexes, Kimberly J. Cope

Dissertations and Theses (Open Access)

The majority of proteins require molecular chaperones to assist their folding into tertiary and quaternary structures. Certain stresses can compromise the weak hydrophobic forces responsible for these structures and lead to protein unfolding, misfolding, and aggregation. Aggregates of proteins are hallmarks of devastating diseases such as Alzheimer’s, Parkinson’s, and Huntington’s diseases. Fortunately, bacteria, plants, and fungi have a potent disaggregase, named Hsp104 in Saccharomyces cerevisiae. Recently, heat-induced aggregates, termed Q-bodies, were found to contain three molecular chaperones: Hsp70, Hsp104, and Hsp42. Their coalescence from small puncta into larger inclusions required Hsp104. During glucose deprivation, a stress that isn’t known to …

The Effect Of Gender And Status Difference On Saturated Fat Content Of Entree Items Chosen By Elementary School-Aged Students, Annie Anderson

All Theses

The purpose of this study was to examine participants in a National School Lunch Program (NSLP), and the effect of gender and/or participation in the NSLP on selection of high saturated fat content entrée items by the students. Nutrition information provided to parents often does not include a profile of the fats offered in school lunch items. This was true of this study's schools. However, new NSLP guidelines focus upon saturated and trans fat content in school lunches. The total kcal limit is less than 30% total fat, and saturated fat is less than or equal to 10% total kcal, …

Food, Nutrition, Culinary, And Packaging Science Undergraduate Research, Experiential Learning, And Product Development Focusing On Childhood Nutrition, Alexandra Weeks

All Theses

The objective of this research project was to develop a two-semester Applied Interdisciplinary Product Development (AIPD) course for sophomore students in the Food, Nutrition and Packaging Sciences (FNPS) department that would increase students’ confidence in skills pertaining to product development of food products and childhood nutrition, increase their sense of connection with the department, and would better prepare them to enter industry than students that did not participate in the course. A Subject Knowledge Assessment (SKA) was used to evaluate the mean difference value (MDV) of food science, nutrition, packaging science, and general product development knowledge gained through the AIPD …

Automatically Measuring Individual Consumption Events During Natural Eating Using A Table Embedded Scale, Ryan Mattfeld

All Theses

This thesis is motivated to improve the tools available for tracking energy intake. The goal of this work is to develop a table-embedded scale capable of measuring the weight of individual consumption events during unrestricted eating. The method was tested on a data set gathered from 276 subjects eating 518 courses consisting of 22,383 marked individual consumption events in a cafeteria environment. Approximately 30% of the consumption events can be detected and weighed. The remaining 70% of the events occur without participants interacting with the scale or when noisy interactions with the scale prevent weight measurement. The relationship between bite …

August 2014 Commencement Program, Bowling Green State University

Commencement Programs

No abstract provided.

An Agent-Based Model Of Ant Colony Energy And Population Dynamics: Effects Of Temperature And Food Fluctuation, Guo Xiaohui

Electronic Theses and Dissertations

The ant colony, known as a self-organized system, can adapt to the environment by a series of negative and positive feedbacks. There is still a lack of mechanistic understanding of how the factors, such as temperature and food, coordinate the labor of ants. According to the Metabolic Theory of Ecology (MTE), the metabolic rate could control ecological process at all levels. To analyze self-organized process of ant colony, we constructed an agent-based model to simulate the energy and population dynamics of ant colony. After parameterizing the model, we ran 20 parallel simulations for each experiment and parameter sweeps to find …

Treatment Of Nitrogen Oxides By Chlorella Vulgaris Algae In Photobioreactors, Steven Shihady

Master's Theses

The effectiveness of algae to treat NO₂and NO in simulated flue gas was tested using Chlorella vulgaris in photobioreactors (PBRs) using NO_xconcentrations between 30 ppm to 780 ppm. NO_xdissolved and reacted in water to form NO₃^-and NO₂^-in the PBR growth medium, providing a nitrogen source that the algae readily assimilated for cell synthesis. Three 20-L photobioreactors were inoculated with a pure culture of C. vulgaris prepared in Bristol growth medium and algae were grown in the PBRs at 25°C and pH of 7.0 in a modified Bristol medium …

A Multicultural Nutrition And Culinary Intervention For Middle School Students: Pink And Dude Chefs, Phase 2, Jaime Lynn Lockhart

Master's Theses

The prevalence of adolescent obesity in the United States has more than quadrupled in the past 30 years, growing from 5% to nearly 21% in 12-19 year olds. Although obesity has many interrelated causes, a lack of knowledge and practical skill to prepare healthy food from scratch is an important factor. Research indicates that nutrition and culinary interventions based on behavioral change theories may improve mediators of healthful eating in adolescents.

Pink and Dude Chefs, Phase 2, an eight-week after-school nutrition and culinary intervention targeted towards middle school students in a predominantly low-income, Hispanic community, was developed and evaluated. The …

Analysis Of High Fat Diet Induced Genes During Mammary Gland Development: Identifying Role Players In Poor Prognosis Of Breast Cancer, Raquel C. Martinez-Chacin, Megan Keniry, Robert Dearth

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Background

Epidemiological studies have shown that consumption of a high-fat diet (HFD) increases the risk of developing breast cancer (BC). Studies in rodents have shown HFD causes changes in the genetic programming of the maturing mammary gland (MG) increasing the susceptibility of developing the disease. Less is known about how HFD induced genes impact BC development. HFD exposure two weeks before conception to six weeks of age was previously shown to dramatically change MG gene expression in 10 week old mice. Therefore, we investigated these differentially expressed HFD-induced genes for their expression in BC using the NKI 295 breast tumor …

Effects Of Water Parameters On Container Mosquito (Diptera: Culicidae) Oviposition And Performance, Stephanie Sue Schelble

Master's Theses

Water body parameters have a considerable effect on the communities that develop within them. In small container habitats like tires, the depth, surface area, and volume effect the development and success of the mosquito communities. This study investigated the choices of the adult female mosquitoes, Aedes albopictus and Culex quinquefasciatus, between different depths and surface areas. In addition, larval performance was determined under differing depth and larvae densities. Oviposition studies showed that Ae. albopictus had a preference for deeper habitats (χ²= 14.2902, p= 0.0139) but did not prefer any surface areas (χ²= 7.2321, p= 0.0649) though …

The Percussionist Bodybuilder: Optimizing Performance Through Exercise And Nutrition, Anthony Joseph Merlino

UNLV Theses, Dissertations, Professional Papers, and Capstones

Do preventive therapies have the potential to help avoid common injuries among percussionists? This document investigates physiological therapy and nutrition in relation to percussion performance. My interest in this subject matter is derived from my personal experiences with injuries related to the performance of percussion. Although I have studied and played percussion professionally for more than a decade, I was unaware of certain methods of rehabilitation that were available to me prior to my injuries. As I learned about the rehabilitation process through first hand experience, I discovered that my injuries were very common among percussionists. This raised the question: …

How Microlending Affects Innovation And Entrepreneurship: Evidence From Ethiopia, Robert Hirth

Graduate Theses and Dissertations

Advocates of microlending suggest it is a sustainable intervention that reaches the poor directly and offers them the means to invest and improve their incomes (Khavul, 2010; Morduch, 1999; Yunus, 2007); yet, impact studies of these interventions have suggested they often have little or even a detrimental impact on borrowers (Van Rooyen, Stewart & De Wet, 2012). This dissertation examines the efforts to promote entrepreneurship and alleviate poverty in developing countries through microlending. I begin by reviewing the microlending literature, and in particular, impact studies of the effect microlending is having in developing countries. Next, I review theory and empirical …

Evaluation Of A Mycorrhizal-Like Fungus, Piriformospora Indica, On Floriculture Crops, Allison Justice

All Dissertations

Piriformospora indica is a fungal endophyte, often called an arbuscular mycorrhizal-like fungus, that has been shown to provide benefits to plant symbionts by increasing nutrient uptake, biomass production, flower number, and disease resistance in a wide range of plant hosts. Research was carried out to investigate the ability of P. indica to improve plant production in floriculture crops. The first objective was to determine the optimal environmental conditions for growing P. indica in pure culture. Environmental conditions were optimized to produce the maximum chlamydospores for inoculum preparation (Chapter 1). These findings were used in the remaining chapters to prepare inocula …

Motivational Factors Contributing To The Choice Of Agriculturally Related Careers, Lacey J. Sims

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study examined the motivational factors of college students who have chosen to study in an agriculturally related field. A descriptive research design using an online questionnaire was used for this study to determine factors which influenced career choice. Likert scale items were used to determine how motivating certain factors were for respondents in influencing their decision to study in an agriculturally related field. The Likert items were divided into five constructs: family influence, self-motivation, experience, use of skills, and career opportunities. Items within the five constructs were further analyzed to discover the top five motivational factors identified in each …

Changes In Body Condition From Prepartum To Postpartum On Health And Reproduction In Dairy Cattle, Keli Ann Akers

Graduate Theses, Dissertations, and Problem Reports (ETD)

The aim of this study were to body condition score, 72 Holstein-Friesian dairy cows at prepartum, compare lean, moderate, and over-conditioned categories, and relate any changes associated with the various time points with reproductive success by measuring body condition score, hematocrit, total white blood cell (WBC) differential, haptoglobin (Hp) concentrations, milk yield and monitoring health status. The body condition score changed significantly with time with the greatest unit loss being in the over-conditioned cattle from d -30 to d 35 postpartum. The BCS losses for each category did not have a significant effect on reproductive success at any time. Health …

Diet Induced Alteration Of The Murine Intestinal Microbiome After Antibiotic Ablation, Adam Irvin

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background and Objective: We detected a dominant non-cultivatable gram-positive coccobacillus associated with cultivatable yeast in feces of mice treated with a cocktail of broad-spectrum antibiotics while being fed a partially defined mouse chow that is commonly used for nutritional studies. The goal of this study was to determine the identity of the non-cultivatable gram-positive coccobacillus.;Methods: Fecal samples were analyzed by Gram stain, quantitative flow cytometry, next generation sequencing of the V3 or V4 regions of the 16S rRNA gene, fluorescence microscopy, and sequence analysis of the tuf gene, which can distinguish among Streptococcus and Staphylococcus genera.;Results: Fecal samples from antibiotic-treated …

Ion Current Rectification In Nano/Micro-Fluidic Interfaces And Pulsed Glow Discharge Time-Of-Flight Mass Spectrometric Chemical Speciation, Han Wang

Graduate Theses, Dissertations, and Problem Reports (ETD)

Microfluidics continues to be of interest for analyzing chemical and biological samples because of the disposability, portability, low sample consumption, fast analysis time, and parallel analysis potential for multiple samples in a single device. To improve microfluidic device functionality, integrated systems-nanofluidic/microfluidic interfaces (NMIs) have been fabricated for concentrating samples and performing as molecular gates. Ion current rectification has been confirmed in NMI with an asymmetric system. In chapter 2, the asymmetry of the NMI is systematically altered by varying the inner diameter of the nanocapillary membrane (NCM) reservoir, and the current rectification factor is observed to increase as the inner …

Heterotrophic Culture Of Microalgae Using Biodiesel Derived Glycerol, Karthik Gopalakrishnan

All Dissertations

Algae-based technologies are fast growing and the growing demand for sustainable technologies is evident from the growing energy demand and global warming. Microalgae culturing for higher lipid contents have been a hot topic of intense discussion in the past years. Crude glycerol, a byproduct from biodiesel production seems to be an attractive feedstock for microbial cultivation and crude glycerol has been proven as a good alternative feedstock for cultivation of Chlorella protothecoides. The effect of impurities present in the crude glycerol is important to develop a method for high-density cultivation of microalgae to increase the commercialization potential of algae systems. …

Platform Technologies To Advance Clinically Relevant Tissue Engineered Heart Valve Products, Leslie Neil Sierad

All Dissertations

Diseased heart valves are commonly replaced by mechanical, bioprosthetic, or allograft heart valves. These replacements provide major improvements in cardiac function and quality of life, but have significant limitations and eventually require surgical replacement within 15-20 years. These risks are particularly prominent in pediatric patients and young adults. The field of tissue engineering and regenerative medicine, which combines scaffolds and cells, holds great promise in developing living replacement heart valves that would self-repair and grow in size along with the growing children.

The long-term goal of this project is to generate living, tissue-engineered heart valves from biological scaffolds and autologous …

Backyard Beekeeping, Rachelle Messner, James Strange, Roslynn Brain

Environment and Society Faculty Publications

In an effort to become more sustainable, small-scale backyard operations by private citizens have become increasingly popular. This fact sheet provides a basic guideline for those interested in backyard beekeeping, whether just for the enjoyment of tending the bees, or the reward of the delicious honey produced. With proper care, management, and harvesting, backyard beekeeping can provide health, economic, and environmental benefits.

Connecting Opportunity: Leaders' Perceptions Of Cross-Sector Collaboration Focused On Disconnected Youth In Westchester County, New York: An Interpretive Phenomenological Analysis, Marian Gryzlo

Education Doctoral

This qualitative study analyzed how leaders from the public, private, philanthropic, and educational sectors view the complex issue of cross-sector collaboration addressing disconnected youth in Westchester County, New York. The research explored participants’ perceptions about this population, also known as opportunity youth, who are ages 16 to 24 and not in school, employed, or on-track to successfully transitioning to adult independence. The study used Interpretive Phenomenological Analysis (IPA) as its methodology to explore, examine, and interpret how leaders perceive the complex and challenging issue of disconnected youth who are living in a suburban county in the New York metropolitan region. …

Management Of Silverleaf Whitefly, Bemisia Argentifolii Bellows And Perring (Hemiptera: Aleyrodidae), Using Non-Crop Companion Plants In Organically-Managed Cantaloupe Systems In South Texas, Ruth Renee Colyer

Theses and Dissertations - UTB/UTPA

Field trials in three separate phases of research were carried out from March, 2010, to August, 2012, to determine the effects of inter-cropping cantaloupe (Cucumis melo var. inodorus) with select non-crop companion plants in an effort to develop organic production strategies for melons in South Texas without the use of pesticides. A research program was designed and initiated to assess the effects of the companion plants and their ability to: 1) attract insect pollinators, and 2) repel or suppress pest species without affecting beneficial natural enemy complexes. The hypotheses were: 1) that organically-managed cantaloupe systems grown with non-crop companion plants …

Empiric Deworming And Cd4 Count Recovery In Hiv-Infected Ugandans Initiating Antiretroviral Therapy, Alexander J. Lankowski, Alexander C. Tsai, Michael Kanyesigye, Mwebesa B. Bwana, Jessica E. Haberer, Megan Wenger, Jeffrey N. Martin, David Bangsberg, Peter W. Hunt, Mark J. Siedner

OHSU-PSU School of Public Health Faculty Publications and Presentations

Background: There is conflicting evidence on the immunologic benefit of treating helminth co-infections (‘‘deworming’’) in HIV-infected individuals. Several studies have documented reduced viral load and increased CD4 count in antiretroviral therapy (ART) naive individuals after deworming. However, there are a lack of data on the effect of deworming therapy on CD4 count recovery among HIV-infected persons taking ART.

Methodology/Principal Findings: To estimate the association between empiric deworming therapy and CD4 count after ART initiation, we performed a retrospective observational study among HIV-infected adults on ART at a publicly operated HIV clinic in southwestern Uganda. Subjects were assigned as having received …

Educating The Whole Child: Evidence Of Health And Wellness Instruction In The Schools Serving Low-Income Students, Andria M. Shook Phd

Dissertations

Educators in the United States are expressing concern about student physical and mental well-being. Childhood obesity has more than doubled in the past three decades, and today, one in five children experiences symptoms of mental illness. Low-income children are especially subject to these conditions. The long-term consequences for the country as a whole are significant. Schools are particularly well placed to address these problems by supporting the well-being of the whole child. Efforts have begun in select schools and districts to incorporate health and wellness instruction into the school day. This study identifies existing health and wellness components that three …

August 2014 News Releases, University Of Montana--Missoula. Office Of University Relations

University of Montana News Releases, 1928, 1956-present

No abstract provided.

Effects Of Breathing Cool Air During Cycling Exercise In The Heat, Christian B. Ridings

Graduate Theses and Dissertations

INTRODUCTION: The purpose of this study was to establish the ability of the Core Cooler device to prevent rises in physiological heat strain of trained male cyclists during cycling exercise in the heat. METHODS: 15 healthy male cyclists cycled at 50-70% VO2max for 75 minutes in a heat chamber of 31°C & 55% RH while breathing through the Core Cooler device under three different conditions: 1:4 ratio without ice termed control (CN), 1:4 ratio with ice termed low intermittent (LI), and at 1:1 ratio with ice termed high intermittent (HI). Data collected every 15 minutes assessing intestinal temperature (TGI), heart …