Digital Commons Network^™

The Microbiome Of Rainbow Trout (Oncorhynchus Mykiss), Liam T. Lowrey

Biology ETDs

Commensal organisms are an integral part of all vertebrates, contributing heavily to development, pathogen defense, and metabolism. Commensals reside at different body sites within vertebrate animals creating unique and distinct communities that vary between locations. The human microbiome project has revealed distinct bacterial community compositions at the diverse range of body sites that have been sampled, providing evidence for different functional purposes of each microbiome. Rainbow trout, Oncorhynchus mykiss, serves as a model organism for the study of mucosal physiology and immunology. Teleost fish are evolutionarily important as one of the first jawed vertebrates with a dedicated adaptive mucosal immune …

Investigation Into The Control Of Melittin Secondary Structure And Antimicrobial Activity, Zachary B. Molinets

Open Access Theses

Antimicrobial resistance has been an exponentially growing problem since the discovery of antibiotics. Antibiotics have been misused for many years and this misuse has grown into a real problem for the medical community. While there are countless safeguards to prevent infection by a resistant strain of bacteria, there are still many plagued by it and must be treated with sometimes dangerous antibiotics. Melittin, along with many other peptides, contain potent antimicrobial properties, but are also toxic toward enthrocytes. The control of the secondary structure of peptides provides the key to adjusting their activity.

Comparative Lca Of Stand-Alone Power Systems Applied To Remote Cell Towers, Eduardo Olmeda Noguera

Open Access Theses

Remote cell towers need on-site power generation or stand-alone power systems for operation and, traditionally, diesel electric generators have been used as backup power systems in combination with renewable energies. Currently, Polymer Electrolyte Membrane Fuel Cells (PEMFC's) are emerging as substitutes for the diesel electric generator, because of their reliability, high-energy conversion efficiency and zero emissions while operating.^ However, very few studies have compared the environmental impact of these two technologies, especially on remote cell tower applications. As PEMFC's have no emissions while operating, concerns about their environmental impact surge because of the use of rare and high processed materials, …

Key Residues Of Human Cytoplasmic Protein Tyrosine Phosphatase-A And -B For Substrate Binding And Specificity, Byunghyun Park

Open Access Theses

Reversible tyrosine phosphorylation plays an important role in signaling pathways that are essential for regulating cellular growth, differentiation and metabolism. Moreover, several human diseases such as diabetes, obesity and cancers are associated with the deregulation of protein tyrosine phosphatases (PTPs). Several studies provide evidence that PTPs not only contribute to cellular differentiation, but over-expression of these molecules also leads to transformation of non-transfomed cells as well. Based on these results, designing specific PTP inhibitors may ultimately function as potential therapeutic agents to treat various diseases including cancer, diabetes, and autoimmune diseases. EphA2 is a receptor tyrosine kinase which is hypo-phosphorylated …

Employment Discrimination, Peter Reed Corbin, John E. Duvall

Mercer Law Review

Following a relatively quiet and uneventful 2012 survey period, the United States Supreme Court stepped up its activity significantly in the area of employment discrimination during the 2013 survey period. The Supreme Court handed down several significant rulings. In University of Texas Southwestern Medical Center v. Nassar, the Court established a "but-for" rule of causation for purposes of Title VII retaliation cases. In Vance v. Ball State University, the Supreme Court determined the parameters of who qualifies as a "supervisor" for purposes of establishing vicarious liability in workplace harassment actions under Title VII.

At the circuit level, the …

Bacteria On Shared Mobile Phones Can Lead To Infections, Roberta Basol, Jean Beckel, Judy Gilsdorf-Gracie, Amy Hilleren-Listerud, Terri Mccaffrey, Sherri Reischl, Pamela Rickbeil, Mary Schimnich, Kirsten Skillings, Mary A. Struffert

Articles

It's now a common practice for hospital-owned mobile phones to be shared among healthcare employees from shift to shift. Despite the benefit of increased, timely communication between caregivers, sharing mobile devices can lead to the spread of healthcare-associated infections (HAIs) if they aren't properly disinfected. The Guidelines for Disinfection and Sterilization in Healthcare Facilities describe "non-critical environmental surfaces" as items that are frequently touched by the hand and may pose a risk of secondary infection transmission.

Understanding The Factors Affecting Microbiological Quality Of Wheat Milled Products: From Wheat Fields To Milling Operations, Luis E. Sabillón Galeas

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Although regarded as a low-risk commodity, wheat flour-based mixes have been implicated in several food safety incidents. The present thesis is a compilation of five scientific manuscripts on the effect of weather variation, milling steps and implementation of pre-milling interventions on the microbiological quality and safety of wheat and milled products. The first manuscript is a review of the microbiological quality and safety of wheat-based products. Despite the low water activity, wheat flour may harbor dormant but viable microorganisms, which could lead to safety concerns when flour is used to produce refrigerated dough products. The second manuscript illustrates the effect …

Vcam-1/Α4Β1 Integrin Interaction Is Crucial For Prompt Recruitment Of Immune T Cells Into The Brain During The Early Stage Of Reactivation Of Chronic Infection With Toxoplasma Gondii To Prevent Toxoplasmic Encephalitis, Qila Sa, Eri Ochiai, Tomoko Sengoku, Melinda E. Wilson, Morgan Brogli, Stephen Crutcher, Sara A. Michie, Baohui Xu, Laura Payne, Xisheng Wang, Yasuhiro Suzuki

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Reactivation of chronic infection with Toxoplasma gondii can cause life-threatening toxoplasmic encephalitis in immunocompromised individuals. We examined the role of VCAM-1/α4β1 integrin interaction in T cell recruitment to prevent reactivation of the infection in the brain. SCID mice were infected and treated with sulfadiazine to establish a chronic infection. VCAM-1 and ICAM-1 were the endothelial adhesion molecules detected on cerebral vessels of the infected SCID and wild-type animals. Immune T cells from infected wild-type mice were treated with anti-α4 integrin or control antibodies and transferred into infected SCID or nude mice, and the animals received the same antibody every other …

Changing Epidemiology Of Tuberculosis Detected By An 8-Year Retrospective Laboratory Study In A Tertiary Teaching Hospital In Central Saudi Arabia, Ali M. Somily, Tahir Naeem, Hanan A. Habib, Mohammed S. Sarwar, Dennis Y. Kunimoto, Abdelmageed M. Kambal

Saudi Medical Journal

OBJECTIVE: To study the laboratory diagnosis of tuberculosis (TB), and relate the findings to its epidemiology in Central Saudi Arabia. METHODS: This retrospective study was carried out at the Department of Pathology/Microbiology, King Khalid University Hospital, Riyadh, Kingdom of Saudi Arabia between January 2003 and December 2010. Data were retrieved from the hospital information system on laboratory findings. After adjustment, 9,405 specimens were studied. The specimens were stained by Ziehl-Neelsen (ZN), auramine-rhodamine, and cultured in Bactec alert 960, and Lowenstein-Jensen media. Mycobacterium tuberculosis (M. tuberculosis) complex and non-tuberculous mycobacteria were differentiated by ProbTec system and p-nitrobenzoate medium. The BACTEC MGIT …

Spatial And Temporal Distribution Of Fungicides Applied To Creeping Bentgrass, Kurt Ronald Hockemeyer

Open Access Theses

Turf managers often rely on fungicides to limit damage caused by root diseases. Since fungicides do not move basipetally, they are effective only when fungitoxic concentrations are delivered to the rhizosphere. This research focused on the distribution of modern fungicides in verdure, thatch, sand, and roots of creeping bentgrass (Agrostis stolonifera L. var. palustris (Huds.) Farw.) maintained as a putting green. Fungicides (azoxystrobin (methyl (E)-2-[2-[6-(2-cyanophenoxy)pyrimidin-4-yloxy]phenyl]-3-methoxyacrylate), propiconazole (1,2,4-Triazole, 1-((2-(2,4-dichlorophenyl)-4-propyl-1,3-dioxolan-2-yl)methyl), pyraclostrobin (carbamic acid, [2-[[[1-(4-chlorophenyl)-1H-pyrazol-3-yl]oxy]methyl]phenyl]methoxy-,methyl ester), and thiophanate-methyl (dimethyl 4,4'-o-phenylenebis[3-thioallophanate]) were applied to replicate field plots in a water volume of 815 L ha-1. Plots were sampled over time (0, 3, 7, 10, …

From 4-H International Youth Exchange To Global Citizen: Common Pathways Of Ten Past Program Participants, Mary E. Arnold, Jamie Davis, Aimee L. Corliss

Journal of Youth Development

This qualitative study examined the life course of ten adults who participated as youth in the 4-H International Exchange program. The purpose of this research was to explore the pathways that led each participant to their exchange experience as a youth and how that experience set the stage for further development into an adulthood marked by cultural interest, awareness, competence and global citizenship. Using a grounded theory approach the data were analyzed through selective, open, and axial codings that elucidated a series of key events, opportunities, and qualities that form a common pathway among all participants. The results of this …

Prevalence Of Manufacturing Defects In Latex Examination Gloves Used In Selected Dental Practices In Central Saudi Arabia, Abdullah S. Al-Swuailem

Saudi Medical Journal

OBJECTIVE: To assess the defect rates in latex examination gloves used in selected dental practices in Riyadh, Saudi Arabia. METHODS: In this cross-sectional study, a total of 796 latex examination gloves were collected from 5 governmental hospitals and 5 private dental practices between April 2012 and May 2012. The gloves were assessed for presence of defects visually (VT) and using water inflation test (WIT). One and 2 sample t-tests were used to assess significant differences in defect rates among each latex brand, and between governmental hospitals and private dental practices. RESULTS: Defects in latex gloves were more likely to be …

The Evaluation Of Essential Oils For Antimicrobial Activity, Dallas Rohraff, Roderick Morgan

Student Summer Scholars Manuscripts

The emergence of antibiotic resistant bacteria is of pressing concern as health care associated infections kill 99,000 people a year in the U.S. alone. Researchers are currently looking for new antibiotics in alternative sources. Essential oils are traditionally known to have medical benefits, and cinnamon, tea tree, and eucalyptus oils have shown antibiotic activity. Initial testing via standard microbiological protocols found minimum inhibitory concentration (MIC) values of 0.039% for cinnamon, 1.25% for tea tree, and 0.313% for eucalyptus. All three oils proved effective against both Gram-positive and Gram-negative bacteria. Cinnamon bark oil, Cinnamomum cassia Blume, appeared most effective. More thorough …

Small-Molecule Probes Targeting The Viral Ppxy-Host Nedd4 Interface Block Egress Of A Broad Range Of Rna Viruses., Ziying Han, Jianhong Lu, Yuliang Liu, Benjamin Davis, Michael S Lee, Mark A Olson, Gordon Ruthel, Bruce D Freedman, Matthias J. Schnell, Jay E Wrobel, Allen B Reitz, Ronald N Harty

Department of Microbiology and Immunology Faculty Papers

Budding of filoviruses, arenaviruses, and rhabdoviruses is facilitated by subversion of host proteins, such as Nedd4 E3 ubiquitin ligase, by viral PPxY late (L) budding domains expressed within the matrix proteins of these RNA viruses. As L domains are important for budding and are highly conserved in a wide array of RNA viruses, they represent potential broad-spectrum targets for the development of antiviral drugs. To identify potential competitive blockers, we used the known Nedd4 WW domain-PPxY interaction interface as the basis of an in silico screen. Using PPxY-dependent budding of Marburg (MARV) VP40 virus-like particles (VLPs) as our model system, …

Saudi Medical Journal 35 Year Anniversary, Ali Albarrak

Saudi Medical Journal

No abstract provided.

A Likelihood Approach To Estimate The Number Of Co-Infections, Kristian A. Schneider, Ananias A. Escalante

Harold W. Manter Laboratory of Parasitology: Library Materials

Article abstract:

The number of co-infections of a pathogen (multiplicity of infection or MOI) is a relevant parameter in epidemiology as it relates to transmission intensity. Notably, such quantities can be built into a metric in the context of disease control and prevention. Having applications to malaria in mind, we develop here a maximum-likelihood (ML) framework to estimate the quantities of interest at low computational and no additional costs to study designs or data collection. We show how the ML estimate for the quantities of interest and corresponding confidence-regions are obtained from multiple genetic loci. Assuming specifically that infections are …

Transcriptome Of The Female Synganglion Of The Black-Legged Tick Ixodes Scapularis (Acari: Ixodidae) With Comparison Between Illumina And 454 Systems, Noble Egekwu, Daniel E. Sonenshine, Brooke W. Bissinger, R. Michael Roe

Biological Sciences Faculty Publications

Illumina and 454 pyrosequencing were used to characterize genes from the synganglion of female Ixodes scapularis. GO term searching success for biological processes was similar for samples sequenced by both methods. However, for molecular processes, it was more successful for the Illumina samples than for 454 samples. Functional assignments of transcripts predicting neuropeptides, neuropeptide receptors, neurotransmitter receptors and other genes of interest was done, supported by strong e-values (<-6), and high consensus sequence alignments. Transcripts predicting 15 putative neuropeptide prepropeptides ((allatostatin, allatotropin, bursicon α, corticotropin releasing factor (CRF), CRF-binding protein, eclosion hormone, FMRFamide, glycoprotein A, insulin-like peptide, ion transport peptide, myoinhibitory peptide, inotocin ( = neurophysin-oxytocin), Neuropeptide F, sulfakinin and SIFamide)) and transcripts predicting receptors for 14 neuropeptides (allatostatin, calcitonin, cardioacceleratory peptide, corazonin, CRF, eclosion hormone, gonadotropin-releasing hormone/AKH-like, insulin-like peptide, neuropeptide F, proctolin, pyrokinin, SIFamide, sulfakinin and tachykinin) are reported. Similar to Dermacentor variabilis, we found transcripts matching pro-protein convertase, essential for converting neuropeptide hormones to their mature form. Additionally, transcripts predicting 6 neurotransmitter/neuromodulator receptors (acetylcholine, GABA, dopamine, glutamate, octopamine and …

Hispanic Residential Ethnic Density And Depression In Post-Acute Coronary Syndrome Patients: Re-Thinking The Role Of Social Support, Ellen-Ge Denton, Jonathan A. Shaffer, Carmela Alcantra, Lynn Clemow, Elizabeth Denton

Publications and Research

Background: The ethnic density hypothesis suggests that ethnic density confers greater social support and consequently protects against depressive symptoms in ethnic minority individuals. However, the potential benefits of ethnic density have not been examined in individuals who are facing a specific and salient life stressor.

Aims: We examined the degree to which the effects of Hispanic ethnic density on depressive symptoms are explained by socioeconomic resources and social support.

Methods: Patients with acute coronary syndrome (ACS, N = 472) completed the Beck Depression Inventory (BDI) and measures of demographics, ACS clinical factors and perceived social support. Neighborhood characteristics, including median …

Outcomes Of Third-Year Pharmacy Students Using Technology For Instruction In An Infectious Diseases Elective Course, Joanna Headley, Pharmd, Candidate 2015, Bhavik Shah, Pharmd, Bcps, Aahivp, Jason J. Schafer, Pharmd, Mph, Bcps, Aahivp

College of Pharmacy Faculty Papers

BACKGROUND

• The use of technology both inside and outside the classroom is integral to the facilitation of pharmacy students’ learning.¹

• Technology is allowing education to restructure classroom time for something other than simple transmission of factual information and to adopt an evidence-based approach to instructional innovation and reform.¹

• Pharmacy colleges and schools should incorporate technologies that students frequently use and that positively impact learning.²

• Limited assessment of the impact of technology on student performance is available including teaching and learning from peers.^1,2

• An infectious diseases elective course developed at the Jefferson School of Pharmacy incorporated the …

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The Prediction Of B-Cell Epitope Via Biostatistical And Bioinformatic Methodology And Applications, Bo Yao

School of Biological Sciences: Dissertations, Theses, and Student Research

By creating antibodies against antigens, B-cells, also named B-lymphocytes, play an important role in the immune system to fight against foreign invasion to the host body. Within the antigen specific to a certain B-cell antibody, the sections recognized and bound by antibody are called B-cell epitopes. As antigenic determinants, B-cell epitope identification is of vital importance in many immunological processes, such as vaccine design, immunodiagnostic tests, and antibody production. Towards this goal, biologists and immunologists have applied a variety of methods to identify B-cell epitopes through both experiments and bioinformatic predictions.

There are two kinds of B-cell epitopes: linear (continuous) …

2014/2015 University Of The Pacific Arthur A. Dugoni School Of Dentistry Catalog, University Of The Pacific

Catalogue, Bulletin, Catalog

No abstract provided.

Growing South Dakota (Summer 2014), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Extending Knowledge, Changing Lives: SDSU Extension Marks 100-Year Milestone
[Page] 5 Preparing for SDSU Extension’s Future
[Page] 5 SDSU College of Agriculture & Biological Sciences Administrative Team [Page] 6 Summer College News
[Page] 7 Profiles In Leadership: Joseph Cassady; Local Leadership Important For Ag’s Future
[Page] 8 Advancing Agriculture: SDSU Precision Ag Program Evolves, Expands [Page] 9 On The Front Line: ADRDL Leads Important Effort For Animal Health Diagnostics
[Page] 10 4-H Philanthropy: Supporters Continue Campaign For New 4-H Exhibit Hall [Page] 11 Growing Global Citizens: AgBio Courses Offer International Learning Opportunities
[Page] 12 Guidance For Gardeners: Several …

A Discrete Density-Dependent Model Of The Solanum Virus, James Morgan

All Student Theses and Dissertations

Compartmental modeling has been used to model infectious diseases for roughly 100 years. Since 2009, several papers have modeled zombie outbreak using this method with various results. This paper will develop a unique model for the spread of the The Walking Dead zombie virus throughout the contiguous United States. Frequency dependent and density dependent transmission will be discussed, and density dependent transmission will be shown to be the appropriate choice for this model. Constant parameters, such as birth rate, bite rate, death rate, and turning rate will be determined using real-world and fictional data. After developing a basic model, modifications …

Evaluation Of An Educational Breastfeeding Program For A Non-Profit Agency In South Texas, Marcela Urbina Zavala

Theses and Dissertations - UTB/UTPA

Breastfeeding has been recognized as the most cost effective practice to promote healthier babies among populations (Dennis, 2002). The purpose of the study was to evaluate the effect of an educational breastfeeding and a prenatal + breastfeeding program for individuals attending group classes and to compare the differences between Spanish only and English only versions of the curriculum. Two research questions were stated to know if there were significant differences between pre and post-tests scores and if there were any significant differences in the impact of the intervention between English only and Spanish only versions of the classes. The present …

Global Burden, Distribution, And Interventions For Infectious Diseases Of Poverty, Zulfiqar Ahmed Bhutta, Johannes Sommerfeld, Zohra S. Lassi,, Rehana A. Salam, Jai K Das

Department of Paediatrics and Child Health

Infectious diseases of poverty (IDoP) disproportionately affect the poorest population in the world and contribute to a cycle of poverty as a result of decreased productivity ensuing from long-term illness, disability, and social stigma. In 2010, the global deaths from HIV/AIDS have increased to 1.5 million and malaria mortality rose to 1.17 million. Mortality from neglected tropical diseases rose to 152,000, while tuberculosis killed 1.2 million people that same year. Substantial regional variations exist in the distribution of these diseases as they are primarily concentrated in rural areas of Sub-Saharan Africa, Asia, and Latin America, with geographic overlap and high …

Factors Associated With Quality Of Life Among Mothers Of Children With Autism Spectrum Disorder, Marian Marconyak

Health Services Research Dissertations

The increasing prevalence of Autism Spectrum Disorder (ASD) has levied associated effects upon the quality of life (QOL) of mothers of children with ASD. These mothers are subjected to various influences upon their lives as a result of their role as caregivers to children with ASD. The purpose of this study was to assess the International Classification of Functioning, Disability and Health (ICF) Body Functions and Structures, Activities, Participation, Environmental, and Personal Factors associated with QOL among mothers of children with ASD.

English literate, inhabitants of the United States, mothers over the age of 18, of children with ASD were …

Mechanistic Models Of Biofilm Growth In Porous Media, Priyank Jaiswal, K. Fathiya Al-Hadrami, Estella A. Atekwana, Eliot A. Atekwana

Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works

Nondestructive acoustics methods can be used to monitor in situ biofilm growth in porous media. In practice, however, acoustic methods remain underutilized due to the lack of models that can translate acoustic data into rock properties in the context of biofilm. In this paper we present mechanistic models of biofilm growth in porous media. The models are used to quantitatively interpret arrival times and amplitudes recorded in the 29 day long Davis et al. (2010) physical scale biostimulation experiment in terms of biofilm morphologies and saturation. The model pivots on addressing the sediment elastic behavior using the lower Hashin-Shtrikman bounds …

Use Of System Dynamics Modeling To Explicate The Theory-Of-Change Of A Social Marketing Innovation, Brian J. Biroscak

USF Tampa Graduate Theses and Dissertations

Community coalitions are an important part of the public health milieu and thus subject to many of the same external pressures as public health organizations--including changes in required strategic orientation. Many funding agencies have shifted their funding agenda from program development to policy change. Thus, the Florida Prevention Research Center created the Community-Based Prevention Marketing for Policy Development framework to teach community coalitions how to apply social marketing to policy change. The dissertation research reported here was designed to explicate the framework's theory-of-change. The research question was: "What are the linkages and connections between CBPM inputs, activities, immediate outcomes, intermediate …

Decreases In Self-Reported Alcohol Consumption Following Hiv Counseling And Testing At Mulago Hospital, Kampala, Uganda, Judith A. Hahn, Robin Fatch, Rhoda K. Wanyenze, Steven Baveewo, Moses R. Kamya, David Bangsberg, Thomas J. Coates

OHSU-PSU School of Public Health Faculty Publications and Presentations

Background: Alcohol use has a detrimental impact on the HIV epidemic, especially in sub-Saharan Africa. HIV counseling and testing (HCT) may provide a contact opportunity to intervene with hazardous alcohol use; however, little is known about how alcohol consumption changes following HCT.

Methods: We utilized data from 2056 participants of a randomized controlled trial comparing two methods of HCT and subsequent linkage to HIV care conducted at Mulago Hospital in Kampala, Uganda. Those who had not previously tested positive for HIV and whose last HIV test was at least one year in the past were eligible. Participants were asked at …

2013-2014 Department Of Internal Medicine Scholarly Activity Report, Quillen College Of Medicine, East Tennessee State University

Quillen College of Medicine Scholarly Activity Reports

No abstract provided.