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Structure-Function Analysis Of Zapc, An Ftsz-Ring Stabilizer, In Escherichia Coli Cytokinesis, Lukasz Tchorzewski

Dissertations and Theses

In Escherichia coli, cell division is defined by the polymerization and constriction of a cytokinetic ring (Z ring) formed by FtsZ, a tubulin-like GTPase, at midcell. Division also involves the formation of a multi-protein complex at midcell known as the divisome. Several divisome proteins promote the assembly/disassembly processes of FtsZ, thereby exercising spatiotemporal control over division. Among FtsZ regulatory proteins are the FtsZ ringassociated proteins (Zap), which either directly or indirectly stabilize the Z-ring by increasing lateral interactions amongst FtsZ protofilaments in the Z-ring. ZapA-D are recruited during early cytokinesis and have overlapping functions in stabilizing FtsZ at midcell, but …

Forecasting The Spread Of Raccoon Rabies Using A Purpose-Specific Group Decisionmaking Process, Aaron M. Anderson, Stephanie A. Shwiff, Richard B. Chipman, Todd Atwood, Tyler Cozzens, Frank Fillo, Robert Hale, Brody Hatch, Joanne Maki, Olin E. Rhodes, Erin E. Rees, Charles E. Rupprecht, Rowland Tinline, Kurt C. Vercauteren, Dennis Slate

Human–Wildlife Interactions

The Centers for Disease Control (CDC) and USDA Wildlife Services (WS) have been involved in an oral rabies vaccination (ORV) program for raccoons (Procyon lotor) that has slowed the westward spread of raccoon rabies. The objective of this study was to forecast the spread of the disease if an ORV zone was not maintained. A group decision-making process was designed to address the forecasting problem and was implemented using a group of 15 experts and 4 support personnel at a meeting at the USDA National Wildlife Research Center. Ten expansion regions were constructed that described the spread of …

Towards Proactive And Flexible Agent-Based Generation Of Policy Packages For Active Transportation, Araz Taeihagh, Rene Bañares-Alcántara

Research Collection School of Social Sciences

One of the approaches gaining ground in policy design is the implementation of combinations of policy measures as policy packages with the aim of increasing efficiency and effectiveness of the designed policies. In this paper, we describe the recent advancements in the developments of a virtual environment for the exploration and analysis of policy packages. The virtual environment uses an agent-based modelling approach for the generation of different configurations of policy measures in the policy packages. The benefit of using the approach is the proactive and flexible generation of policy packages as the agents can react to the changes that …

Post Exposure Prophylaxis Among Ugandan Nurses Accidents Do Happen, J. Mill, Esther Nderitu, S. Richter

School of Nursing & Midwifery, East Africa

Purpose: In 2009 we conducted a study to explore Ugandan nurses’ practice of universal precautions while caring for persons living with HIV. During our interviews about universal precautions, nurses’ also shared their experience with post-exposure prophylaxis (PEP) following needle-stick injuries. We present findings related to nurses’ understanding of PEP and their experience with, and reporting of, needle stick injuries.

Background: Nurses have high rates of exposure to blood-borne pathogens. Although there is minimal risk of the transmission of blood-borne pathogens from health care workers (HCWs) to patients and vice versa, post-exposure prophylaxis, has become routine following the occupational exposure of …

Clinical Manifestations, Complications, And Treatment Of Brucellosis: Evaluation Of 72 Cases, Şükran Köse, Süheyla Seri̇n Senger, Gülgün Akkoçlu, Lütfi̇ye Kuzucu, Yildiz Ulu, Gürsel Ersan, Fi̇li̇z Oğuz

Turkish Journal of Medical Sciences

Brucellosis is an important disease in developing countries. We aimed to determine the epidemiologic, clinical, and laboratory characteristics of brucellosis, which still has a high morbidity in Turkey. Materials and methods: Seventy-two patients with brucellosis, monitored at our clinic from January 2004 to July 2010, were reviewed retrospectively. Results: The average age was determined to be 44.8 ± 18 years, and 40 of the patients were female (55.6%). The most frequent transmission route was the use of raw milk and dairy products, in 45 of the patients (62.5%). The most frequent complaints were joint pain, high fever, weakness, low back …

Thromboelastography In The Evaluation Of Coagulation Disorders In Patients With Sepsis, Yeli̇z Kiliç, İsmet Topçu, Hamza Bambal, Melek Çi̇vi̇

Turkish Journal of Medical Sciences

Unbalanced hemostasis and disseminated intravenous coagulopathy serve as key participants in organ dysfunction and disability. In this study we evaluated the coagulation profiles of patients diagnosed with systemic inflammatory syndrome (SIRS)-sepsis and multiple organ dysfunction syndrome. We also researched coagulation in sepsis by comparing thromboelastography (TEG) data with those of nonsepsis patients to determine the usefulness of the TEG device. Materials and methods: Data were collected from 55 anesthesiology and surgery intensive care unit (ICU) patients: 21 with SIRS-sepsis (Group S) and 34 patients without SIRS-sepsis (Group C). Blood samples were taken upon admission to the ICU (t1) and on …

Glut 4 And Insulin Resistance, Salmin Ali

Browse all Theses and Dissertations

Five types of glucose transporters stimulate the transport of glucose from the bloodstream into cells such as muscle cells and adipocytes in response to insulin signaling or exercise. Skeletal muscle and adipose tissue are the main sites for glucose uptake and dispose in insulin action dependent pathway. In non-insulin stimulated condition, the GLUT4 storage vesicles stay away from the plasma membrane, so there is no GLUT4 translocation or GLUT4 mediated glucose uptake. However, in insulin stimulated state, insulin initiates insulin signaling cascade which results in rapid GLUT4 translocation and increased glucose uptake at the cell surface. Failure of the translocation …

A Step In The Right Direction Or Side-Stepping The Issue: Is State Regulation For Factory Farming Appropriate?, Cynthia Furmanek

Student Works

No abstract provided.

Hormonal Contraceptives Alter The Immune Function And Structural Integrity Of The Female Genital Tract: Implications For Hiv-1 Infection, Katherine Gisella Michel

All ETDs from UAB

The potential effect of hormonal contraception on HIV-1 acquisition and transmission represents an important public health issue. Several observational studies have suggested an association between the use of hormonal contraception, in particular injectable depot medroxyprogesterone acetate (DMPA), and an increased risk of HIV-1 acquisition and transmission. To delineate the biological mechanisms behind this phenomenon, we have recruited healthy, HIV-negative women using either DMPA, NuvaRing, or combined oral contraceptives for a vaginal biopsy and cervicovaginal lavage (CVL). Analysis of the functional capacity of circulating plasmacytoid dendritic cells (pDCs), distribution of vaginal immune cell populations, and systemic and genital levels of immune …

Structural Implications Of P22 Bacteriophage Coat Protein A-Domain Modifications, David Samuel Morris

All ETDs from UAB

Modification of virus capsids as target-specific delivery devices has become highly popularized in recent years and P22 bacteriophage has been proposed as a potential candidate for development of a targeting drug delivery system. As a proof of concept for our system, we hypothesized that the A-domain of P22 coat protein could be utilized to alter the binding affinity of virus-like procapsids for biological targets. Addition of acidic residues to the A-domain revealed that this could be accomplished through the quarternary localization of charged residues in three-dimensional space without interrogating procapsid assembly. Further manipulations implemented in vivo were performed to examine …

Characterization Of Glycosylation-Associated Protein, Gap2, Required For The Biogenesis Of Streptococcus Parasanguinis Fimbriae-Associated Protein, Fap1, Haley Echlin

All ETDs from UAB

Streptococcal species possess a multitude of adhesins that facilitate adherence to a wide range of substrates; this is the first step necessary for the development of disease. Streptococcus parasanguinis is a primary colonizer of the oral cavity and adheres to the tooth surface using long fimbriae, which are composed of Fap1, a serine-rich repeat glycoprotein (SRRP). SRRPs are conserved in many Gram-positive bacteria and play a role in bacterial adhesion, fimbrial formation, biofilm formation, and bacterial pathogenesis. Although SRRPs play such an important role, the exact mechanism of their biogenesis remains a mystery. For Fap1, an eleven gene cluster is …

Innate Immune Defense Against The Fungal Pathogen Aspergillus Fumigatus, Melissa Ann Gessner

All ETDs from UAB

The opportunistic fungal pathogen Aspergillus fumigatus is a ubiquitous mold that causes severe, invasive life-threatening infections in patients that are severely immunocompromised. Major risk factors include neutropenia, neutrophil dysfunction and immunosuppressive therapies. Recently, however, rising incidences of colonizing A. fumigatus have been reported in patients without classical risk factors. We sought out to elucidate non-classical pre-disposing conditions that may render a lung environment susceptible to fungal colonization as well as mechanisms involved in immune defense against the invading fungal pathogen. Further defining a role for Dectin-1 in an Aspergillus fumigatus infection, we demonstrated a critical role for Dectin-1 dependent interleukin-22 …

Using Mce4 Sirna Molecular Beacons For Detection And Eradication Of Mycobacterial Infection In Macrophages, Remo George

All ETDs from UAB

temperatures. The third chapter represents the most comprehensive evaluation to date of

Assessing The Active Kinome Of Influenza Virus Infected A549 Cells, Colm Atkins

All ETDs from UAB

Influenza A Virus is a respiratory pathogen of the family Orthomyxoviridae and causes significant worldwide morbidity and mortality during seasonal epidemics and periodic pandemics. As with all viruses, influenza's limited coding capacity requires the use of host proteins and processes in its replication and release cycle. Phosphorylation, mediated by cellular kinases and phosphatases, represents a significant mechanism of post-translational modification of cellular products, and is responsible for regulating the activi-ty, lifespan and localization of many lipids and proteins in host-cells. Several cellular kinases have been associated with various steps of the Influenza lifecycle, including PKC-ßII (viral entry), PKR (host defense), …

Cutting Effectiveness Of Diamond Burs On Dental Zirconia., Ching-Kai Lin

All ETDs from UAB

Monolithic zirconia restorations are popular but removing these crowns is difficult. The purpose of the study is to measure and compare the cutting effectiveness of 10 types of diamond burs through monolithic sintered zirconia. Ten types of diamond burs (n=8) were compared for the cutting effectiveness in Zirconia (CERCON; DeguDent) using a high speed computer controlled cutting device with water spray (5.45L/min). The turbine used to cut the blocks rotated at 150,000 rpm and with the burs at a depth of 2mm into the zirconia during cutting. A 100 gm load was applied during cutting for each bur which made …

Adapting Like The Animals: The Endangered Species Act As A Model For Human Adaptation To Climate Change, Meghan C. Boland

Brooklyn Journal of International Law

No abstract provided.

Mobile Phone Short Message Service (Sms) To Improve Malaria Pharmacoadherence In Zambia, Elinda Steury

Electronic Theses and Dissertations

Malaria significantly contributes to morbidity and mortality rates in Zambia. The currently accepted malaria treatment is artemisinin-based combination therapy (ACT); it is more than 97% effective when the regimen is strictly adhered to. However, the mean ACT adherence rate in sub-Saharan Africa is only approximately 38-48%. Poor pharmacoadherence remains a significant barrier to malaria control and elimination. The purpose of this study was to determine if adherence rates to a six-dose ACT antimalarial treatment differ between patients in Zambia who received short message service (SMS) reminders and those who did not. An experimental, randomized, controlled trial was conducted to collect …

Modeling Risk For Sexually Transmitted Infections In Women In A Court-Ordered Substance Treatment Program, Frances Deavers

Electronic Theses and Dissertations

Developing a comprehensive model of Sexually Transmitted Infection (STI) risk factors and their inter-relationships is vital to improving methods of risk identification and treatment delivery. The CDC posed three general categories that may serve as a framework for such a model: sexual network, individual behavior, and social/ structural risk. None of the extant risk models incorporate measures from all three categories. Additionally, none of these models, generally focused on individual behavior, use medical data on infection as their outcome variable. This is problematic because the ultimate outcome of infection is also influenced by sexual network and social/ structural variables, in …

Diet At Medieval Alytus, Lithuania: Stable Carbon And Nitrogen Isotope Analysis Of Bone And Dentin Collagen, Katie Whitmore

Electronic Theses and Dissertations

The Baltic region was a vibrant center of power and economic prosperity in medieval Europe; Lithuania in particular. Until now, little stable isotopic analysis has been utilized to assess diet in this region during this time period. The aim of this study was to undertake a preliminary assessment of the composition of diet at late medieval Alytus (late 14th to early 18th centuries) from bone (N=35) and dentin (N=38) collagen samples. The stable carbon isotopic data suggest a diet primarily comprised of C3 plants such as barley, rye, wheat, and flax, and animals consuming C3 plants. The stable nitrogen isotopic …

Purification And Effectiveness Of Vaccines And Antiviral Compounds, Maria F. Gencoglu

Dissertations, Master's Theses and Master's Reports - Open

Viral infections account for over 13 million deaths per year. Antiviral drugs and vaccines are the most effective method to treat viral diseases. Antiviral compounds have revolutionized the treatment of AIDS, and reduced the mortality rate. However, this disease still causes a large number of deaths in developing countries that lack these types of drugs. Vaccination is the most effective method to treat viral disease; vaccines prevent around 2.5 million deaths per year. Vaccines are not able to offer full coverage due to high operational costs in the manufacturing processes. Although vaccines have saved millions of lives, conventional vaccines often …

2013-2014 Che Newsletter, Department Of Chemical Engineering, Michigan Technological University

Department of Chemical Engineering Newsletters

Table of Contents

• Department
• Research
• Student News
• Faculty News
• Alumni & Friends

When Law Reform Is Not Enough: A Case Study On Social Change And The Role That Lawyers And Legal Clinics Ought To Play, Jeff Carolin

Journal of Law and Social Policy

Based on his experience as a law student in the clinical legal education program at Parkdale Community Legal Services in 2010, the author draws on poverty law scholarship to better understand his frustrations with a law reform campaign he worked on related to refugee family reunification. The scholarship’s central critique of law reform campaigns is that they are excessively narrow: they focus on a particular law and construct the law itself as the social injustice. This leads to two subsidiary problems. First, law reform campaigns ignore the underlying socio-political context that produced the law, foregoing opportunities for broader societal transformation. …

Public Health Emergencies: What Counts?, Lawrence O. Gostin

Georgetown Law Faculty Publications and Other Works

Vaccines and drugs to prevent and treat Ebola Virus Disease that have never been tested in humans, and in scarce supply raise profound ethical challenges. What if good evidence emerged demonstrating safety and efficacy of drugs? What would be an ethical method of allocating scarce beneficial resources? The apparent preference given to foreign aid workers over West Africans provoked a firestorm. In addition to discussing the ethical allocation of scarce drugs, this article also asks a more fundamental question: Why did it take nearly 40 years after the first Ebola outbreak in 1976 to launch clinical trials?

Getting Into The Meat Of The Word Of Wisdom, A. Jane Birch

Interpreter: A Journal of Latter-day Saint Faith and Scholarship

In verse 13 of the Word of Wisdom, the Lord tells us, “it is pleasing unto me that they [flesh of beasts and fowls of the air] should not be used, only in times of winter, or of cold, or famine” (D&C 89:13). Judging from the variety of interpretations this single verse has inspired, it would appear to be deeply enigmatic. Interestingly, most interpretations have been put forward with little supporting evidence. This article is the first comprehensive analysis of the diverse explanations for D&C 89:13 that have been suggested since 1833. In this article, I attempt to analyze these …

Characterization Of The Effects Of Iron On Neisseria Gonorrhoeae Surface Protein Modulation And Host Cell Interactions, Aminat Oki

Theses and Dissertations

Iron is an essential nutrient that is sequestered by iron-binding proteins in the human host resulting in a hostile environment for microbes. Neisseria gonorrhoeae, however, can utilize numerous iron-binding proteins such as transferrin and lactoferrin to acquire this nutrient. During initial infection, gonococci have access to transferrin and lactoferrin present in semen and vaginal fluids, as well as to hemoglobin present in blood during menses or disseminated infections. Consequently, the gonococcus likely encounters conditions of high iron at some stages in the course of natural infection. Potential contributions of iron to gonococcal invasion have however been largely over looked in …

The Role Of Toll-Like Receptor 9 In Periodontitis, Paul Kim

Theses and Dissertations

Periodontitis is a biofilm-initiated inflammatory disease, resulting in soft tissue damage and alveolar bone loss. A nucleic acid sensor, toll-like receptor 9 (TLR9), has been recently implicated in periodontal inflammation. This study utilized an in vivo periodontitis model using TLR9 knockout (TLR9-/-) mice to assess the role of TLR9 in periodontitis. Significant bone loss was observed in wild type, but not in TLR9-/- mice. Further experiments using ex vivo assays revealed significantly higher IL-6 production in splenocytes of wild type mice compared to knockout cells in response to Porphyromonas gingivalis (a keystone pathogen for periodontitis) challenge. In conclusion, TLR9 contributes …

A Route To Discover Small Molecule Inhibitors Of Psaa, A Potential Target For Streptococcus Pneumoniae, Ahmad J. Obaidullah

Theses and Dissertations

Due to the development of multidrug resistance in Streptococcus pneumoniae, research has begun to define new drug targets for pneumonia therapy. Different research groups have identified a lipoprotein, PsaA that is important for pneumonia virulence. PsaA is a manganese transporter that is required for bacterial virulence and growth. We have employed computer modeling to virtually screen a small-molecule database for inhibition of PsaA function by targeting the metal binding pocket, performing receptor-based virtual screening and molecular docking and scoring to identify potential inhibitors of PsaA function. We have developed an assay for screening compounds, including the use of a PsaA …

The Influence Of Dementia Caregiver Mental Health On Quality Of Care In Argentina, Alejandra Morlett Paredes

Theses and Dissertations

This study examined the connections between cognitive functioning in individuals with dementia and caregiver burden, burden and mental health, mental health and quality of care. One hundred two dementia caregivers from San Lucas, Argentina completed questionnaires assessing these constructs. Caregiver burden, depression, anxiety, and satisfaction with life explained 18.8% of the variance in quality of care – respect and 14.7% of the variance in quality of care – provide. An SEM with generally adequate fit indices uncovered that cognitive functioning in individuals with dementia was inversely associated with caregiver burden, caregiver burden was inversely associated with mental health, and mental …

Democratic Deliberation And The Ethical Review Of Human Subjects Research, Govind Persad

Publications

In the United States, the Presidential Commission for the Study of Bioethical Issues has proposed deliberative democracy as an approach for dealing with ethical issues surrounding synthetic biology. Deliberative democracy might similarly help us as we update the regulation of human subjects research. This paper considers how the values that deliberative democratic engagement aims to realize can be realized in a human subjects research context. Deliberative democracy is characterized by an ongoing exchange of ideas between participants, and an effort to justify decisions that bind participants by appeal to reasons that the participants can understand and share. Even when unanimous …

The Syntheses, Characterizations, And Applications Of Novel Photo- And Electrochemical Catalysts, Lauren Rae Grabstanowicz

Graduate Research Theses & Dissertations

This dissertation investigates two separate clean energy applications, emphasizing material applications and electrochemistry techniques. The first section examines environmentally friendly alternatives for water purification to avoid carcinogenic by-products. To diminish such possibilities, research has shifted towards photoelectrochemical catalysts, such as titanium dioxide (TiO2), because pure TiO2 has limited absorption in the ultraviolet region. Therefore, Ti3+, a self-dopant, has become an attractive candidate for environmentally friendly alternatives. This dissertation demonstrates a novel oxidation procedure to form self-doped Ti3+ in the bulk of rutile TiO2. A brief overview of the work will be given and supporting data including material, methods, structural characterization, …