Digital Commons Network^™

Laboratory Colonisation And Genetic Bottlenecks In The Tsetse Fly Glossina Pallidipes, Marc Ciosi, Daniel K. Masiga, Charles M.R. Turner

All Peer-Reviewed Publications

Background:The IAEA colony is the only one available for mass rearing of Glossina pallidipes, a vector of human and animal African trypanosomiasis in eastern Africa. This colony is the source for Sterile Insect Technique (SIT) programs in East Africa. The source population of this colony is unclear and its genetic diversity has not previously been evaluated and compared to field populations.Methodology/Principal Findings:We examined the genetic variation within and between the IAEA colony and its potential source populations in north Zimbabwe and the Kenya/Uganda border at 9 microsatellites loci to retrace the demographic history of the IAEA colony. We performed classical …

Annual Giving: Recommended Practices For Universities Based On Current Literature, Conversations With Professionals, And Personal Experience, Paige Berry

Honors College Theses

Development, or fundraising, is essential to non-profit institutions. Hospitals, universities, and charity groups rely on the support of individuals, corporations, foundations, and government agencies to fund their endeavors. Educational fundraising has existed in America as long as the higher education system itself. Private institutions like Harvard College originally gained funding by sending solicitors from America to England. (Worth, 1993, p. 18) Throughout the years, departments that focus exclusively on development have been established in universities and non-profits, with subdivisions focused on various constituent segments. At educational institutions, alumni relations departments often form partnerships with development offices because of the similarities …

Adverse Health Effects Of Spousal Violence Among Women Attending Saudi Arabian Primary Health-Care Clinics, H. M. Eldoseri, K. A. Tufts, Q. Zhang, J. N. Fish

Ellmer School of Nursing Faculty Publications

This study aimed to investigate the frequency of spousal violence among Saudi women and document the related health effects and injuries, as well as their attitudes to gender and violence. Structured interviews were conducted with 200 ever-married women recruited from primary-care centres in Jeddah. Nearly half of the surveyed women (44.5%) reported ever experiencing physical violence from their spouse. Although 37 women (18.5%) had received violence-related injuries, only 6.5% had reported these injuries to a health-care provider. Victims of spousal violence had poor perceptions of their overall health, and reported pain or discomfort, antidepressant use and suicidal thoughts. Women mostly …

Telehealth: Preparing Advanced Practice Nurses To Address Healthcare Needs In Rural And Underserved Populations, Carolyn M. Rutledge, Tina Haney, Michele Bordelon, Michelle Renaud, Christianne Fowler

Ellmer School of Nursing Faculty Publications

Healthcare is being confronted with questions on how to deliver quality, affordable, and timely care to patients, especially those in rural areas, in systems already burdened by the lack of providers. Advanced Practice Registered Nurses (APRNs) have been challenged to lead this movement in providing care to these populations through the use of technologies, specifically telehealth. Unfortunately, APRNs have limited exposure to telehealth during their educational experience, thereby limiting their understanding and comfort with telehealth. To address this problem, a telehealth program was developed at a large university that prepares Doctor of Nursing Practice (DNP) APRN students. The telehealth program, …

Predicting Post-Deployment Family Adaptation In U.S. Navy Families, Micah A. Scott, Esther H. Condon, Arlene J. Montgomery, Spencer R. Baker

Ellmer School of Nursing Faculty Publications

Background: Although military families worldwide face changes that include adapting to peace and wartime deployments, few studies have explored how military families adapt to the post-deployment return of a service member.

Objectives: To identify variables that predicted post-deployment adaptation of U.S. Navy families.

Methods: A mixed method study guided by the Roy Adaptation Model included a convenience sample of 142 spouses of service members recently returned from deployment. The degree to which length of deployment, prior deployments, and years married, number of children, participation in religious and family support groups, communication, race, and interdependence predicted post-deployment family adaptation was tested. …

A Systems Biology Approach To Detect Eqtls Associated With Mirna And Mrna Co-Expression Networks In The Nucleus Accumbens Of Chronic Alcoholic Patients, Mohammed Mamdani

Theses and Dissertations

Alcohol Dependence (AD) is a chronic substance use disorder with moderate heritability (60%). Linkage and genome-wide association studies (GWAS) have implicated a number of loci; however, the molecular mechanisms underlying AD are unclear. Advances in systems biology allow genome-wide expression data to be integrated with genetic data to detect expression quantitative trait loci (eQTL), polymorphisms that regulate gene expression levels, influence phenotypes and are significantly enriched among validated genetic signals for many commonly studied traits including AD.

We integrated genome-wide mRNA and miRNA expression data with genotypic data from the nucleus accumbens (NAc), a major addiction-related brain region, of 36 …

Symptoms, Cytokines, And Quality Of Life Of Patients With Chronic Graft-Versus-Host Disease Following Allogeneic Hematopoietic Stem Cell Transplantation, Debra Kelly

Theses and Dissertations

Introduction: Chronic graft-versus-host disease (cGVHD) is a serious complication following allo-HSCT characterized by immune dysregulation, organ dysfunction, risk for infection, and distressing symptoms. Complications may include scleroderma, hepatic dysfunction and bronchiolitis obliterans. Advances in allo-HSCT for many hematologic dyscrasias (e.g. acute and chronic leukemias, aplastic anemia, and myelodysplastic syndrome) have improved survival which has generated a renewed focus on survivorship issues. Distressing symptoms are noted as negatively impacting quality of life (QoL). The relationship between inflammation and behavioral responses may impact symptoms. Examining patterns and levels of inflammation with symptoms is relevant. Objective: The aims of this study were to …

Identifying Organizational Containment And Its Effect On The Career Paths Of Black Educators, Risha Berry

Theses and Dissertations

The racial composition of the school workforce is the product of a complex interaction of social forces. School constituencies such as parents, students, and teachers impact new leader preferences. Their ideas influence decisions regarding who fits into school administration and who doesn't (Williams & Loeb, 2012). These "fit" preferences can shape school district ethos, which then influences access to social networks (Tooms, 2010). Limited entry to these networks may produce multiple disadvantages, particularly for Black educators. One way to think of this access is through the concept of containment. Restricted data from the 2011-12 Schools and Staffing Survey (SASS) will …

Latent Variable Models Given Incompletely Observed Surrogate Outcomes And Covariates, Chunfeng Ren

Theses and Dissertations

Latent variable models (LVMs) are commonly used in the scenario where the outcome of the main interest is an unobservable measure, associated with multiple observed surrogate outcomes, and affected by potential risk factors. This thesis develops an approach of efficient handling missing surrogate outcomes and covariates in two- and three-level latent variable models. However, corresponding statistical methodologies and computational software are lacking efficiently analyzing the LVMs given surrogate outcomes and covariates subject to missingness in the LVMs. We analyze the two-level LVMs for longitudinal data from the National Growth of Health Study where surrogate outcomes and covariates are subject to …

Atm, Brca1, And Aurora A: Mechanisms Of G2/M Checkpoint Control In Human Embryonic Stem Cells, Jason Beckta

Theses and Dissertations

When cultured in vitro, human embryonic stem cells (hESCs) acquire genetic abnormalities that have slowed their therapeutic use. As hESCs have a “leaky” G1/S boundary, the pressure of ensuring genetic integrity falls on the G2/M checkpoint, which can be activated by failed chromosomal decatenation (among other stimuli). It is hypothesized that hESCs have a deficient decatenation checkpoint, but little data supports this. Evidence suggests that the ataxia telangiectasia mutated (ATM) kinase controls the G2/M decatenation and DNA damage checkpoints, though previous reports are conflicting on this point. My work demonstrates that inhibition of decatenation activates ATM and arrests hESCs in …

An Analysis Of Fatty Acid Metabolism’S Role In The Development Of Acute Functional Tolerance To Ethanol In Caenorhabditis Elegans, Richard Raabe

Theses and Dissertations

An individual’s naïve level of response (LR) to ethanol is predictive of their lifetime likelihood to abuse alcohol. LR is heavily genetically influenced, suggesting that the genes responsible for LR may also be central to the development of abuse disorders. Our laboratory uses the model organism C. elegans to investigate the genetic influences on responses to acute ethanol exposure. We recently found that changes in TAG levels can alter LR. From this result we investigated the role of long-chain polyunsaturated fatty acids (LC-PUFAs) as well enzymes involved in lipid modifications of proteins. We found that LC-PUFAs are necessary for acute …

“Rampant Signs And Symbols”: Artifacts Of Language In J.D. Salinger’S “For Esmé—With Love And Squalor” And Glass Family Stories, Courtney Sviatko

Theses and Dissertations

This thesis explores the use of language in J.D. Salinger’s “For Esmé—With Love and Squalor,” “A Perfect Day for Bananafish,” and Raise High the Roofbeam, Carpenters. It establishes a narrative pattern in which sensitive individuals such as Seymour Glass and Sergeant X are isolated by the insensitivity of the superficial modern world, attempt to communicate their concerns to others through an exchange of language in material forms, and ultimately find relief in silence. By analyzing various examples of linguistic artifacts and the impact they have on both sender and receiver, this thesis identifies criteria for successful communication as well as …

To Destroy Or To Preserve: Urban Renewal And The Legal Foundation Of Historic District Zoning, Andrew Tarne

Theses and Dissertations

Historic preservation and urban renewal are often thought to be polar opposites. Where one seeks to preserve, the other generally seeks to destroy in order to rebuild. While the programs appear on the surface to be in opposition, this Thesis seeks to demonstrate that there is a fundamental connection between the underlying legal principles of historic zoning and urban renewal. To that end, the jurisprudence involving historic zoning and aesthetic regulations before and after the seminal urban renewal case of Berman v. Parker has been collected and analyzed. This analysis revealed that courts were hesitant to support aesthetic, and by …

The Cytotoxic Effect Of The Bcl-2 Family Of Proteins In Breast Cancer Cells, Yamileth Chin

Theses and Dissertations

Breast cancer is the second leading cause of death amongst women ages 20 to 59. Despite advancements in cancer therapies, more research is necessary to improve the diagnoses and treatment of several types of breast cancer. Paclitaxel (Taxol) is a commonly utilized anti-cancer drug for various types of solid tumors. However, the molecular mechanism utilized by paclitaxel to induce cell death is still elusive. Previous studies in our laboratory have shown that the pro-apoptotic BCL-2 family protein, BAK (BCL-2 homologous antagonist/killer) plays an important role in paclitaxel-induced cell death. In untreated breast cancer cells, BAK is associated with the anti-apoptotic …

Characterization Of Complement Evasion Of The Periopathogen, Treponema Denticola, Daniel Patrick Miller

Theses and Dissertations

Periodontitis is a polymicrobially-induced, chronic inflammatory disease of the tissues that support and surround the tooth. While greater than 500 organisms are found in dental plaque, Treponema denticola is one of only a few species associated with disease. It has been hypothesized that oral bacteria disrupt host homeostasis through manipulation of the innate immune system. In this study, we examine the impact of binding and subsequent cleavage of factor H, a complement regulator, by T. denticola in the evasion and subversion of complement. The molecular interaction between the sole FH-binding protein, FhbB, and FH was detailed by x-ray crystallography, site-directed …

Hiv And Opiates-Mediated Neurotoxicity: Gsk3Β Is A Potential Therapeutic Target, Ruturaj Masvekar

Theses and Dissertations

HIV-1 enters the CNS soon after initial systemic infection. HIV-1 can induce a wide range of neurological deficits, collectively known as HIV-1-associated neurocognitive disorders (HAND). Mature neurons are not infected by HIV-1; instead, infected and/or activated glial cells release various viral and cellular factors that induce direct and/or indirect neuronal toxicity, leading to HAND. Injection drug abuse is a significant risk factor for HIV-infection, and opiate drug abusers show increased HIV-neuropathology, even with anti-retroviral treatments. Our previous work has largely modeled HIV-neuropathology using the individual viral proteins Tat or gp120, with murine striatal neurons as targets. To model disease processes …

Folate Conjugated Dendrimers For Targeted Anticancer Therapy, Shannon Andrews

Theses and Dissertations

Anticancer therapeutics are often limited to suboptimal doses due to their lack of selectivity for tumor cells and resultant damage to healthy tissue. These limitations motivated researchers to develop tumor-specific delivery systems for improved therapeutic efficacy and reduced unintended cytotoxicity. Polyamidoamine dendrimers offer an ideal platform for designing targeted therapeutics with tunable characteristics that optimize pharmacokinetic behavior and targeting specificity. Ligand conjugation to dendrimer provides the biochemical interaction necessary to activate tumor-specific receptors for receptor-mediated endocytosis and effective internalization of polyplexes. Tumor-specific receptors overexpressed in carcinomas, like folate receptor-alpha (FOLRα), are targeted by ligand-conjugated dendrimer to allow enhanced internalization of …

Evaluating The Antibody Response Against Ospa, Ospc, And Ospe Recombinant Proteins Over Time In Borrelia Burgdorferi Positive Serum Samples, Bridget Dillon

Theses and Dissertations

Lyme disease is the most common tick-borne illness in North America. It can be hard to detect using blood tests, especially in the early stage. Due to the number of significant complications from untreated or undertreated Lyme disease, better methods need to be found to detect the disease. Some surface lipoproteins may be used to detect early disease due to their early expression. Others are maintained for the duration of the infection and can be used to detect chronic Lyme disease. Antibody responses to OspA, OspC and OspE were measured in sera from experimentally infected dogs. The response to OspA …

Characterization Of Tonb-Dependent Metal Transporters Within Neisseria Gonorrhoeae, Mary Kathryne Dickinson

Theses and Dissertations

Neisseria gonorrhoeae, the etiologic agent of gonorrhea, utilizes TonB-dependent transporters to import essential nutrients such as iron. Study of TonB-dependent transporters is extremely important due to the fact that they make excellent vaccine targets. In order to learn more about the structure, function, expression, and regulation of selected TonB-dependent transporters, three goals were established for this study. The first goal was to examine the role of two highly conserved regions of TbpB in lipidation. One of the conserved regions of TbpB, the LSAC motif, was shown to be critical for lipidation. The second goal was to determine whether MisR/MisS regulates …

Understanding The Impact Of Regulatory Changes On The Implementation Of Therapeutic Day Treatment: A Case Study Approach, Angie Mann-Williams

Theses and Dissertations

Therapeutic Day Treatment (TDT) is a community-based mental health treatment program regulated and funded by the Department of Medical Assistance Services (DMAS) in the Commonwealth of Virginia. This case study sought to understand how DMAS regulatory changes impacted the implementation of the TDT program in the Commonwealth between fiscal years 2004 and 2011. In an effort to respond to this question, sources of qualitative and quantitative data were collected including: TDT fee-for-service data, regulations in the Community Mental Health and Rehabilitative Services manual guiding the implementation of the TDT program, and structured interviews with eight key stakeholders who interface with …

Characterization Of A Putative Tonb Deficient Porphyromonas Gingivalis Mutant, Soheil Rostami

Theses and Dissertations

Porphorymonas gingivalis is one of the major bacterial pathogens responsible for the initiation and progression of periodontal disease. The bacterium requires hemin uptake for its growth and has developed sophisticated mechanisms to extract hemin from hemin containing proteins in the oral cavity. Hemin first binds to receptors on the surface of P. gingivalis and is then taken up in an energy dependent manner. TonB is an inner membrane bound protein that spans the periplasm and is believed to be involved in the passage of hemin through the double membrane of P. gingivalis. However, the TonB protein in P. gingivalis is …

The Total Picture: Multiple Chemical Exposures To Pregnant Women In The Us – An Nhanes Study Of Data From 2003 Through 2010, Teri Cabana

Theses and Dissertations

INTRODUCTION: Chemical exposures to US pregnant women have been shown to have adverse health impacts on both mother and fetus. A prior paper revealed that US pregnant women in 2003-2004 had widespread exposure to multiple chemicals. The goal of this research is to examine how environmental chemical exposures to US pregnant women have changed from 2003 to 2010 and to look further at the extent of simultaneous exposure to multiple chemicals in US pregnant women using biomonitoring data available through NHANES (the National Health and Nutritional Examination Survey). METHODS: Using available NHANES data from the following cycles (2003-2004, 2005-2006, 2007-2008, …

Android-Based Smartphone Application Simulation And Systematic Design To Reduce Medication Administration Error In Prehospital Emergency Care., Natalie Vazquez

Theses and Dissertations

Since 1999 when the report To Err is Human: Building a Safer Health System was released, medical errors have come into focus (Kohn, 2000). In an effort to reduce medication administration errors in prehospital emergency care, an android-based smartphone application simulation was created. The app has components including QR barcode scanning, text to speech for medication cross-checking, weight-based medication dose calculations, and time stamped medication data wirelessly transferring to a database in real-time. Color standard identification was implemented, aiding to a designed systematic process for patient treatment to reduce medication errors. Direct observation was performed of emergency patient calls with …

Multi-Species Gene Networks And Drosophila Ethanol Sedation, Arnavaz Kollah

Theses and Dissertations

Alcohol use disorders (AUDs) are major health issues with few known genetic explanations. This project used the fruit fly (Drosophila melanogaster) model to identify genes and gene networks that influence alcohol intoxication, a phenotype related to alcohol abuse in humans. We used bioinformatic tools to build gene networks based on 24 published Drosophila ethanol-responsive genes with human orthologs. We then assessed the role of these networks in ethanol sedation by testing two of the networks seeded on IP3K2, a gene that regulates calcium signaling, and CG14630, a gene involved in carnitine biosynthesis. We knocked down several genes in each of …

Predicting The Academic Functioning Of College Students With Attention-Deficit/Hyperactivity Disorder: The Importance Of Executive Functions And Parent Report, Melissa Dvorsky

Theses and Dissertations

This study examined the impact of several dimensions of executive functioning (EF), as well as Attention-Deficit/Hyperactivity Disorder (ADHD) symptoms, in relation to college students’ academic and overall functional impairment. Participants were 62 college students comprehensively diagnosed with ADHD and their parents/guardians who completed measures of symptoms of ADHD, EF, school maladjustment and functional impairment. The primary goal of the study was to evaluate whether parent- and self-ratings of EFs completed at the beginning of the school year longitudinally predict end of the school year academic and overall functioning above and beyond symptoms of ADHD. Mediation analyses controlling for covariates, including …

Identifying Functional Variation In Schizophrenia Gwas Loci By Pooled Sequencing, Erik Loken

Theses and Dissertations

Schizophrenia demonstrates high heritability in part accounted for by common simple nucleotide variants (SNV), rare copy number variants (CNV) and, most recently, rare SNVs Although heritability explained by rare SNVs and CNVs is small compared to that explained by common SNVs, rare SNVs in functional sequences may identify specific disease mechanisms. However, current exome methods do not capture a large proportion of potentially functional bases where rare variation may impact disease risk: as much as two-thirds of conserved sequences lie outside the exome in non-coding regions of cross-species evolutionary constraint. We reasoned that the candidate loci from the Psychiatric Genomics …

Dr. Who?: The Science And Culture Of Medical Wear Design, Patricia Duignan

Theses and Dissertations

The multi-million-dollar medical uniform industry has not utilized advancements in garment and textile technology that could positively impact the protection of healthcare professionals and patients. In most cases the uniforms meet basic requirements – they clothe the professional in a recognizable way. Little innovation in design, function and performance, has been applied to these garments. This is particularly evident in the case of the stereotypical white lab coat worn by many physicians, despite evidence indicating that these lab coats may carry contamination and play a role in the spread of deadly bacteria. Healthcare Associated Infections (HAIs) are among the most …

Transformation Of Human Mast Cells By Interferon-Gamma And The Potential Role Of Myeloid Derived Suppressor Cells In Mastocytosis., Sahar Lotfi-Emran

Theses and Dissertations

Mast cells respond to a variety of signals, are associated with both increased inflammation and regulation of the immune response, and are able to interact with a variety of hematopoietic and non-hematopoietic cells. The majority of the work that highlights mast cell pleiotropic abilities has been completed in murine models. Though these models have significantly advanced our understanding of what mast cells can do, they cannot inform us as to what mast cells actually do in human beings. The goal of this dissertation is to assess fully mature, primary human mast cell function beyond the well-defined type 1 hypersensitivity function …

Rhetorical Ripples: The Church Of The Subgenius, Kenneth Burke & Comic, Symbolic Tinkering, Lee A. Carleton

Theses and Dissertations

Humor has long been an effective way to engage difficult sociopolitical topics in a way that avoids polemical confrontation and provides opportunity for pleasure, catharsis and self-knowledge. In the context of today’s polarized politics and protest, creative satirical performance that deploys “symbolic tinkering” can provide a “comic frame of reference” that, according to Kenneth Burke, more effectively conveys its message while providing reflexive insight. The satirical Church of the SubGenius naturally practices this rhetorical frame in their multimedia creations. Using the lens of Burke’s Attitudes Toward History, this essay is an analysis of SubGenius rhetoric with a focus on …

Transportation Safety In Virginia: Positive Changes And Future Prospects, Nouran Hakami

Theses and Dissertations

Measured by the level of transportation safety, the Commonwealth of Virginia stands out from all the States because despite increased need for mobility, it manages to maintain its safety indices at exceptionally good levels. In many respects we can attribute this success to the comprehensive Strategic Highway Safety Plan of Virginia (SHSP), which is, as concluded from the analysis of its analogues, among the best in the US. The programs and policies described in this document embrace all aspects of transportation safety and create a harmonious system. To assess the effectiveness of the SHSP, this thesis used correlation and regression …