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The Effect Of Allopurinol And Inosine Administration On Xanthine Oxidoreductase Gene Expression, Mitochondrial Respiration, And Inflammation In Broiler Chickens, Tabatha Settle

Graduate Theses, Dissertations, and Problem Reports (ETD)

Birds have a remarkable longevity for their body size despite an increased body temperature, higher metabolic rate, and increased blood glucose concentrations. Theoretically, birds should sustain a much higher degree of oxidative damage yet do not, in part due to the powerful antioxidant, uric acid. As the end-product of purine degradation, uric acid is generated in the xanthine/hypoxanthine reactions catalyzed by xanthine oxiodreductase (XOR). In the first study, inosine, a purine precursor, was fed to 3 groups of 5 birds: Group 1 was fed 0 (control), Group 2, 0.6 mols inosine/kg feed (INO) and Group 3, INO treatment plus 50 …

Nerve Growth Factor Regulates Na+ Transport In Human Airway Epithelial Cells, Michael J. Shimko

Graduate Theses, Dissertations, and Problem Reports (ETD)

Nerve growth factor (NGF) was discovered for its ability to enhance nerve growth, but recent evidence suggests there is a correlation between elevated NGF levels in the lung and airway diseases, including lung inflammatory diseases and respiratory virus infections. NGF can be produced and act upon both structural and non-structural cells of the airways, and overexpression of NGF causes morphological and physiological changes in the airways, such as an increased innervation resulting in a neuronal remodeling of the lung, airway smooth muscle thickening, increased vascularization, airway hyperreactivity to capsaicin, and subepithelial thickening. Although work has been conducted to investigate NGFs …

Functional Characterization Of Phosphodiesterase-4a In Behavior And Obesity, Rolf T. Hansen Iii

Graduate Theses, Dissertations, and Problem Reports (ETD)

Cognitive disorders such as depression, anxiety, and senescent-induced memory loss are a growing pandemic in the world. Anxiety is the most common psychiatric disorder in America, affecting upwards of 50 million people a year; depression has a lifetime prevalence of 20% of the population, and it's predicted pathological memory disorders such as Alzheimer's disease (AD) will reach exponential levels by the year 2050. While there are treatments for anxiety and depression, far less success has been reached with therapeutics for AD. Phosphodiesterases (PDEs) may be a potential novel target for future therapeutics aimed at combatting these psychiatric illnesses and neurodegenerative …

A Preliminary Study Evaluating Potential Probiotic Use In Endodontics, Khaled Seifelnasr

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction: The main goal of endodontics is the prevention of apical periodontitis. This condition is the result of persistent pathogenic microorganisms such as Enterococcus faecalis (E. f) and Candida albicans (C. a) remaining in the root canal systems of teeth, and the ability of those organisms to directly cause acute and chronic inflammation in the periapical tissues. The concept of the use of probiotics has not yet been evaluated in addressing endodontic disease, but probiotics have proven successful in treating periodontal disease. Taking these findings into account, this preliminary work was performed to evaluate the possible effectiveness of a probiotic …

Diet Induced Alteration Of The Murine Intestinal Microbiome After Antibiotic Ablation, Adam Irvin

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background and Objective: We detected a dominant non-cultivatable gram-positive coccobacillus associated with cultivatable yeast in feces of mice treated with a cocktail of broad-spectrum antibiotics while being fed a partially defined mouse chow that is commonly used for nutritional studies. The goal of this study was to determine the identity of the non-cultivatable gram-positive coccobacillus.;Methods: Fecal samples were analyzed by Gram stain, quantitative flow cytometry, next generation sequencing of the V3 or V4 regions of the 16S rRNA gene, fluorescence microscopy, and sequence analysis of the tuf gene, which can distinguish among Streptococcus and Staphylococcus genera.;Results: Fecal samples from antibiotic-treated …

Animal Dispersal Of American Ginseng (Panax Quinquefolius L.), Amy M. Hruska

Graduate Theses, Dissertations, and Problem Reports (ETD)

American ginseng (Panax quinquefolius L.) is a long-lived forest herb found throughout Appalachia, well-known for its reputation as a medicinal plant species. Harvest of ginseng for the international medicinal plant trade has been a significant contributor to population decline of the species. As a declining species with life-history traits similar to many other herbaceous perennials and both cultural and economic value, American ginseng has become a focal species for many demographic and conservation based studies. Seed dispersal is a critical component of ginseng demography lacking empirical evidence for potential dispersal mechanisms. To date, gravity is considered to be the primary …

Effects Of Response-Independent Food Pellet Delivery On The Development Of Tolerance To The Rate-Decreasing Effect Of D-Amphetamine In Rats, Christopher A. Krebs

Graduate Theses, Dissertations, and Problem Reports (ETD)

The extent to which initial drug administration decreases the ability of an organism to obtain reinforcement can affect the development of behavioral tolerance. Environmental enrichment affects many drug-related phenomena, but its effects on the development of tolerance are not clear. The present study examined how enriching the environment by providing non-contingent food pellets during experimental sessions affects the development of tolerance to a dose of d-amphetamine that produces a loss in reinforcement. Lever pressing for food pellets was maintained under a multiple schedule consisting of two variable-interval (VI) 60-s components. One component was enriched by providing food pellets non-contingently under …

Does Cultural Self-Awareness Alter Instructional Design Decisions?, Antoinette Hando

Graduate Theses, Dissertations, and Problem Reports (ETD)

This project looks at the influence background culture has on instructional designers. Current research supports the hypothesis that background culture affects learning preferences. This study approaches cultural background from the instructional designers' point of view. Using Hofstede's Model of Cultural Dimensions as a tool for understanding how culture influences our expectations in a classroom setting a survey was sent to subjects from various cultures to see if their design style correlated to the ranking given by Hofstede. The results were that background culture influenced the experiences of the participants and could be related to Hofstede's Model of Cultural Dimensions.

Explicit Disclosure In Retail Communication: Impact Of Undetermined Social Network Site Relationships On Branded Identity, Hannah R. Gnegy

Graduate Theses, Dissertations, and Problem Reports (ETD)

One of the most impactful technologies to emerge from Web 2.0 developments has been the social network site. In traditional models of branded identity, it is assumed that there is a distinction made between the retailer and consumer (de Chernatony, 1999; Kapferer, 2008). However, social network sites allow for the formation of undetermined relationships in which the underlying role of a party may be unknown.;As social network sites continue to shape retail practices, a gap in knowledge exists regarding how models of branded identity need to be modified to meet the demands of these undetermined social network site relationships. Through …

Development Of A Remote Laboratory With The Integration Of Cloud Applications, Jeremy Thompson

Graduate Theses, Dissertations, and Problem Reports (ETD)

Distance learning has been a mode of education since its inception at Pennsylvania State University in 1892. What started as a simple correspondence course has blossomed into a globally accepted method of education. The need for remote laboratories is growing as more lab-intensive programs of study are searching for a distance learning alternative. The goal of this research is to establish the foundation of a remote laboratory in the Mechanical and Aerospace Engineering Department at West Virginia University. The main objectives are to develop simple, easy-to-use graphical user interfaces to enhance the understanding of several concepts presented in the MAE …

The Evaluation Of Emotion Regulation In Caregivers Referred To A Parent-Training Program, Nancy M. Wallace

Graduate Theses, Dissertations, and Problem Reports (ETD)

In recent years, emotion regulation has become a topic of particular interest to both clinicians and researchers and has been shown to play a significant role in a variety of behavioral and psychological difficulties in both adults and children (Gratz & Tull, in press). However, parents of children referred for parent training may experience significant difficulty regulating their emotions as they attempt to cope with and control their children's misbehavior. The present study explored the role of caregiver emotion regulation in parent training by attempting to understand if (1) parents and children who are referred for parent training have difficulty …

Saints, Sinners, And Socialists On The Southside: Polish Catholic Immigrant Workers, Politics, And Culture In Wheeling, West Virginia, 1890-1930, William Hal Gorby

Graduate Theses, Dissertations, and Problem Reports (ETD)

In the years after the Civil War, Wheeling, West Virginia developed into a major manufacturing center in the Northern Panhandle. Until 1885, Wheeling was the state capital of West Virginia and was the center of cut nail production in America. The city already possessed a large population of Irish and German immigrants. However, with the decline of the nail industry and the transition to steel manufacturing in the Upper Ohio Valley, by the 1890's, Wheeling attracted many immigrants from Southern and Eastern Europe. These immigrants took their places at the bottom of the industrial hierarchy and were relegated to the …

The Effects Of Chronic Stress And Exercise On Mouse Pancreatic Islet Of Langerhans Morphology And Muscle Atrophy Gene Expression, Travis Parkulo

Graduate Theses, Dissertations, and Problem Reports (ETD)

Chronic stress has been implicated as a possible contributing factor to the onset of Type 2 Diabetes Mellitus through its negative effects on peripheral insulin sensitivity and the insulin producing beta cells of the pancreas. Exercise could serve as a possible countermeasure to the damaging effects of chronic stress due to its proven ability to increase insulin sensitivity at the level of the muscle and improve or maintain beta cell function and mass. To date, no published research has examined the effects of chronic stress in combination with voluntary wheel running on murine islet morphology and skeletal muscle atrophy. The …

The Influence Of 4-H Judging Team Participation On Career Life Skills Development, Evan H. Anderson

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of the study was to determine whether participation in WV 4-H agricultural judging teams impacted life skills development. This study measured the current life skill abilities of past 4-H state judging team winners from 1995 to 2006, determined their perception of the influence involvement on a judging team had on that ability, and compared that with the importance of the various life skills in participants' current jobs. A descriptive survey was sent to the target population, who were winners of West Virginia state 4-H agricultural judging team competitions between the years of 1995 and 2006 and competed in …

Grazing Systems, Edward Barrow Rayburn

Faculty & Staff Scholarship

WVU-Extension fact sheet

Why Use Management-Intense Grazing-Mig, Edward Barrow Rayburn

Faculty & Staff Scholarship

WVU-Extension fact sheet

Regulation Of L1 Expression And Retrotransposition By Melatonin And Its Receptor: Implications For Cancer Risk Associated With Light Exposure At Night., Dawn Deharo, Kristine J Kines, Mark Sokolowski, Robert T Dauchy, Vincent A Streva, Steven M Hill, John P Hanifin, George Brainard, David E Blask, Victoria P Belancio

Department of Neurology Faculty Papers

Expression of long interspersed element-1 (L1) is upregulated in many human malignancies. L1 can introduce genomic instability via insertional mutagenesis and DNA double-strand breaks, both of which may promote cancer. Light exposure at night, a recently recognized carcinogen, is associated with an increased risk of cancer in shift workers. We report that melatonin receptor 1 inhibits mobilization of L1 in cultured cells through downregulation of L1 mRNA and ORF1 protein. The addition of melatonin receptor antagonists abolishes the MT1 effect on retrotransposition in a dose-dependent manner. Furthermore, melatonin-rich, but not melatonin-poor, human blood collected at different times during the circadian …

The Murray Ledger And Times, August 1, 2014, The Murray Ledger And Times

The Murray Ledger & Times

No abstract provided.

Disturbance And Clonal Reproduction Determine Liana Distribution And Maintain Liana Diversity In A Tropical Forest, Alicia Ledo, Stefan A. Schnitzer

Biological Sciences Faculty Research and Publications

Negative density dependence (NDD) and habitat specialization have received strong empirical support as mechanisms that explain tree species diversity maintenance and distribution in tropical forests. In contrast, disturbance appears to play only a minor role. Previous studies have rarely examined the relative strengths of these diversity maintenance mechanisms concurrently, and few studies have included plant groups other than trees. Here we used a large, spatially explicit data set from Barro Colorado Island, Panama (BCI) to test whether liana and tree species distribution patterns are most consistent with NDD, habitat specialization, or disturbance. We found compelling evidence that trees responded to …

Analysis Of High Fat Diet Induced Genes During Mammary Gland Development: Identifying Role Players In Poor Prognosis Of Breast Cancer, Raquel C. Martinez-Chacin, Megan Keniry, Robert Dearth

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Background

Epidemiological studies have shown that consumption of a high-fat diet (HFD) increases the risk of developing breast cancer (BC). Studies in rodents have shown HFD causes changes in the genetic programming of the maturing mammary gland (MG) increasing the susceptibility of developing the disease. Less is known about how HFD induced genes impact BC development. HFD exposure two weeks before conception to six weeks of age was previously shown to dramatically change MG gene expression in 10 week old mice. Therefore, we investigated these differentially expressed HFD-induced genes for their expression in BC using the NKI 295 breast tumor …

Vocabulary Growth Using Nonfiction Literature And Dialogic Discussions In Preschool Classrooms, Gwen R. Marra

Faculty Work Comprehensive List

The preschool years are a crucial time for children to develop vocabulary knowledge. A quality preschool environment promotes large amounts of language usage including picture book read alouds and discussions. There is growing research to support the use of nonfiction literature in preschool classrooms to promote vocabulary growth and knowledge of the world for preschool children. This research study compared vocabulary growth of preschool children using fiction and dialogic discussions versus vocabulary growth of preschool children using nonfiction and dialogic discussions following a six week study of autumn and changes that happen during this season to the environment and animals. …

Cytotoxic Effect Of Salvadora Persica Extracts On Human Gingival Fibroblast Cells, Hanan A. Balto, Khaled K. Al-Manei, Talal M. Bin-Mohareb, Zahid A. Shakoor, Solaiman M. Al-Hadlaq

Saudi Medical Journal

OBJECTIVES: To assess the cytotoxic potential of Salvadora persica (S. persica) extracts on human gingival fibroblast (HGF) cells. METHODS: This study was conducted between January and May 2012 in collaboration with Dental Caries Research Chair, College of Dentistry, King Saud University, Riyadh, Saudi Arabia. Extracts of S. persica using hexane, ethylacetate, and ethanol as solvents at concentrations of 0.5 mg/ml and 1 mg/ml were evaluated for their cytotoxic activity against HGFs using the 3 cytotoxic assays: (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole) (MTS), lactic dehydrogenase (LDH), and crystal violet (CV). International standards for the evaluation of medical materials recommended cut-off value of …

Diagnostic Clues For Spondylitis In Acute Brucellosis, Nazlim Aktug-Demir, Servet Kolgelier, Serap Ozcimen, Sua Sumer, Lutfi S. Demir, Ahmet C. Inkaya

Saudi Medical Journal

OBJECTIVES: To determine the diagnostic factors for brucellar spondylitis. METHODS: This retrospective study included 227 consecutive brucellosis patients admitted to the Infectious Diseases and Clinical Microbiology clinics of Adiyaman State Hospital and Adiyaman 82nd Year State Hospital, Adiyaman, Turkey between January 2010 and December 2012. Acute brucellosis was diagnosed by standard tube agglutination test and/or growth of Brucella spp. in appropriately prepared culture media (Bactec). Brucellar spondylitis was diagnosed and followed-up with contrast-enhanced magnetic resonance imaging. RESULTS: Among the 227 brucellosis patients included, 88 (38.8%) were male, and 139 (61.2%) were female. Brucellar spondylitis was detected in 54 patients (23.7%). …

Social Tipping Points And Earth Systems Dynamics, R.A. Bentley, E. Maddison, P. Ranner, J. Bissell, C. Caiado, P. Bhatanacharoen, Timothy Adrian Robert Clark, Botha M., Akinbami F., Hollow M., Michie R., Huntley B., Curtis S., Garnett P.

Research Collection Lee Kong Chian School Of Business

Recently, Early Warning Signals (EWS) have been developed to predict tipping points in Earth Systems. This discussion highlights the potential to apply EWS to human social and economic systems, which may also undergo similar critical transitions. Social tipping points are particularly difficult to predict, however, and the current formulation of EWS, based on a physical system analogy, may be insufficient. As an alternative set of EWS for social systems, we join with other authors encouraging a focus on heterogeneity, connectivity through social networks and individual thresholds to change.

Integrating Art Education And Literacy Education: A Curriculum For The Secondary Level, Erin Moody-Zoet

Masters Theses

The purpose of this paper is to examine, through the design of a new curriculum, how the integration of reading and writing literacy into a visual arts curriculum can strengthen literacy skills as well as foster creative thinking at the middle school level. There is a growing demand for the integration of literacy in all core, and non-core curricula. By developing a curriculum that integrates literacy strategies in a visual arts program, as well as touches on the disconnect that I see between individuals and their own creative outlets, a curriculum is established that fosters and engages students in creative …

Emerging Technologies In Art Education, Molly A. Marshall

Masters Theses

The purpose of this study is to determine the emerging technologies in visual arts education and the impact they will make on the future of visual arts education in a K-12 setting. Research was done through a literature review. As a result, it was found that integrating technology into the visual arts is beneficial to both student and teacher on many levels.

The research will cover how these technologies affect best practices in teaching. Also how these technologies influence student learning. You will find the history of how technology has been implemented in art education, to give the reader and …

The Evolution Of Genomic Imprinting: Theories, Predictions And Empirical Tests, M M. Patten, L Ross, J P. Curley, David C. Queller, R Bonduriansky, J B. Wolf

Biology Faculty Research

The epigenetic phenomenon of genomic imprinting has motivated the development of numerous theories for its evolutionary origins and genomic distribution. In this review, we examine the three theories that have best withstood theoretical and empirical scrutiny. These are: Haig and colleagues’ kinship theory; Day and Bonduriansky’s sexual antagonism theory; and Wolf and Hager’s maternal–offspring coadaptation theory. These theories have fundamentally different perspectives on the adaptive significance of imprinting. The kinship theory views imprinting as a mechanism to change gene dosage, with imprinting evolving because of the differential effect that gene dosage has on the fitness of matrilineal and patrilineal relatives. …

Because A Fire, A Lover's Quarrel: Poems, Clifton Clyde Ward

Master's Theses

This thesis comprises poems written and/or revised over the past two years. They mark the development of my aesthetic and writing ability thus far and are representative of the training and practice incited by my own efforts and those of my professors during my enrollment in the Master of Arts degree in English, Creative Writing emphasis. The majority of these poems are written in an open form, and all of them fall under three general categories: autobiographical, absurdist, or formal.

A Description Of Vocalizations And Their Association With Mouthing Behaviors And Social Context In Bottlenose Dolphins, Tursiops Truncatus, Christina Elyse Perazio

Master's Theses

Dolphin communication is multimodal and incorporates physical behaviors and vocalizations. Dolphins often exchange information with conspecifics using different types of vocalizations, and these vocalizations are sometimes associated with specific behaviors. However, the relationship of vocalization type and mouthing behavior type has not been investigated. This thesis examines simultaneous acoustic and visual recordings of bottlenose dolphins (Tursiops truncatus) to determine the relationship between type of mouthing behavior and type of vocalization (whistle, whistle-squawk, chirp, moan, burst-pulse type A, burst-pulse type B, and click trains). The role of the social context of a mouthing behavior is also evaluated. Data were obtained opportunistically …

The Ontogeny Of Whistle Production In Infant Atlantic Bottlenose Dolphins (Tursiops Truncatus) During The First Thirty Days Of Life, Brittany Leigh Jones

Master's Theses

The manner in which dolphin calves acquire their whistle repertoire is largely unknown. This paper focuses on whistle development in four bottlenose dolphin calves during the first thirty days of life in order to increase our understanding of the early emergence of whistles and whistle-like vocalizations. The acoustic parameters of whistle-type vocalizations (i.e., whistles and whistlesquawks) that coincided with a bubblestream emission from the focal calf and/or its mother were analyzed, as were the behavioral states of the mother-calf pair during the emission of such vocals. Mother and calf whistle rates are inversely related, with the mother whistling more often …