Digital Commons Network^™

Impedance Biosensors For The Rapid Detection Of Viral And Bacterial Pathogens Using Avian Influenza Virus Subtypes H5n1 And H7n2 And Escherichia Coli O157:H7 As Model Targets, Jacob David Lum

Graduate Theses and Dissertations

This research investigated impedance biosensors for the rapid detection of viral and bacterial pathogens using avian influenza virus (AIV) subtypes H5N1 and H7N2 and Escherichia coli O157:H7 as the model targets, which were chosen due to their impact on the agricultural and food industries. For the detection of AIV H7N2, a single stranded DNA aptamer was selected using systematic evolution of ligands by exponential enrichment (SELEX). The selected aptamer and a previously selected aptamer against AIV H5N1 were used in a microfluidics chip with an embedded interdigitated array microelectrode to fabricate an impedance biosensor for specific detection of AIV H7N2 …

Il-12 Induced Modulation Of Tumor Derived Myeloid Cell Inflammatory Responsiveness., Courtney Jetun Mitchell

Electronic Theses and Dissertations

Tumor infiltrating and tumor associated myeloid cells (TIMs and TAMs) elaborate an array of factors that promote tumor growth and metastasis. IL-12, a potent inflammatory cytokine has been shown to induce regression of many cancers. We hypothesize that IL-12 augments the ability of TIMs and TAMs to respond to inflammatory stimuli providing a window in which these stimuli are more likely to promote tumor destruction. Related to this hypothesis, we asked two questions: (1) Does IL-12 directly change signaling events associated with inflammatory signal transduction? (2) Is IFN? required for the entirety of IL-12 induced enhancement of the response of …

The Learning Pathways Of Ironman Triathletes: Case Studies Of Age-Group Ironman Triathletes, Ari Zelmanow

Dissertations

The Ironman Triathlon is an epic endurance event consisting of a 2.4-mile swim, a 112-mile bike ride, and a 26.2-mile run. There is paucity in the literature relating to how athletes learn how to negotiate this event. This qualitative study was conducted over a 9 month period, to align with the 2013 Ironman training and racing season. Seven athletes were selected for participation in the study. Utilizing a case study approach, the Ironman athletes’ learning pathways were examined through in-depth interviews and audio and video content personally captured by the participants. The learning pathways revealed the athletes initially learned through …

Mapping The Human Vasculature By In Vivo Phage Display, Julianna Bronk

Dissertations and Theses (Open Access)

In vivo phage display screenings by intravenous injection of a random phage-displayed peptide library allow for the selection of peptides that localize to specific vascular beds. At the University of Texas MD Anderson Cancer Center, we have had the opportunity to perform phage display screenings in cancer patients in order to select for cancer specific targets directly in humans. These targets serve to define biochemical diversity of endothelial cell surfaces and can be validated and explored towards the design of vascular-targeted pharmacology. In the most recent patient screen, samples were recovered from hepatocellular carcinoma (HCC) as well as 26 additional …

Pi3k- And Mtor-Dependent Mechanisms Of Lapatinib Resistance And Resulting Therapeutic Opportunities, Samuel Brady

Dissertations and Theses (Open Access)

Breast cancers with HER2 amplification represent 20-25% of breast cancer cases and are frequently responsive to the HER2 kinase inhibitor lapatinib, but generally for only short duration. We aimed to understand how breast cancers with HER2 amplification become resistant to lapatinib, in order to identify potential therapies that can overcome lapatinib resistance. To establish lapatinib resistance models we treated three HER2+ breast cancer cell lines with lapatinib for several months until they became lapatinib-resistant. We then compared lapatinib-sensitive (parental) cells with their lapatinib-resistant (LapR) counterparts to identify changes conferring lapatinib resistance. We found that activation of PI3K, specifically the p110α …

Characterization Of The Vascular Pathology In The Acta2 R258c Mouse Model And Cerebrovascular Characterization Of The Acta2 Null Mouse, Carlos A. Villamizar

Dissertations and Theses (Open Access)

Mutations in ACTA2, the gene encoding for smooth muscle α-actin, predispose patients to a wide range of vascular diseases and is most commonly associated with thoracic aortic aneurysms (TAAD) and dissections (TAAD) and strokes. TAAD in patients is characterized by aortic media degeneration and loss in contractile force generation followed by aortic enlargement and subsequent rupture. Most stroke cases associated with ACTA2 are described as moyamoya-like cerebrovascular incidents characterized by distal occlusion of the internal carotid artery, stenosis of arteries in the Circle of Willis, and straightening of cerebral arteries. Patient samples are scarcely available for analysis, and the few …

Role Of The Ang-Tie2 Pathway In The Invasive Recurrence Of Gbm Following Anti-Vegf Therapy, Nahir Cortes Santiago

Dissertations and Theses (Open Access)

Strong pre-clinical and clinical data supporting the effectiveness of bevacizumab, a humanized monoclonal anti-VEGF antibody, for the treatment of gliomas led to its accelerated approval for the treatment of patients with recurrent glioma. However, despite strong anti-tumor effects, upon treatment with bevacizumab, patients will invariably recur with a tumor characterized by enhanced invasiveness and resistance to therapy. This study aims to elucidate the mechanisms leading to this enhanced malignancy with the hope of uncovering new potential therapeutic targets for combined treatment. Using tissue sections from U87-derived glioma bearing mice treated with or without aflibercept (another anti-VEGF antibody) we have gathered …

Biomass Scoping Study: Opportunities For Agriculture In Western Australia, Kim Brooksbank, Mitchell Lever, Harriet Paterson, Melissa Weybury

Bulletins 4000 -

This 2014 report aims to provide a summary of national and international activity in the use of agricultural by-products for the production of bioenergy and biofuels. The summary is primarily an internal report for the Department of Agriculture and Food, Western Australia (DAFWA), but will hopefully be of some value to industry proponents that are interested in pursuing the opportunities provided by what are currently low value agricultural waste products. We outline three processes for obtaining energy from these by-products that may be appropriate for the farming sector in Western Australia (WA).

3-D Morphometric Analysis Of The Primate Elbow Joint, Seth Brockman Boren

Graduate Theses and Dissertations

Large body size requires limb joints capable of supporting said weight, and a species exhibiting sexual size dimorphism may necessitate joint size differences between the sexes of the species. If habitual behavior differs with body size, one may expect to see significant variation in joint morphology between species and the sexes within species. The following analysis tests two hypotheses: (1) that significant differences in joint size between males and females correlate with the magnitude of sexual dimorphism and (2) that there is significant interspecific variance in joint shape between males and females of the same species. The first hypothesis is …

Microvascular Topology And Intravascular Endothelial Cell Labeling In The Gracilis Anterior Muscle Of Balb/C Mice, Paul Heckler Ii

Biomedical Engineering

Peripheral arterial occlusive disease (PAOD) affects approximately 200 million individuals globally. The major underlying cause of PAOD is an inflammatory disease known as atherosclerosis, which results from the build-up of low-density lipoproteins (LDL) in the sub-intimal space. This initiates a complex cascade of events that lead to plaque growth. Plaque growth can then expand into the lumen of the vessel and result in occlusion and/or thrombosis. Symptoms of the disease can include claudication, ulcers, and/or gangrene, although many patients are asymptomatic. Similar to other forms of ischemic disease, risk factors for PAOD include hypertension, diabetes, and smoking. Common treatments include …

The Environmental And Health Costs Of Alternative Diets: A Comparative Study Of The U. S. Diet Relative To The French, Japanese, Mediterranean, And Nordic Diets, Sarah Rehkamp

Department of Agricultural Economics: Dissertations, Theses, and Student Research

This thesis contributes to the literature on sustainable consumption by using scenario analysis to evaluate the environmental and health costs of the U.S. diet relative to the French, Japanese, Mediterranean, and Nordic diets, identified in the literature as healthier diets. As a first step in estimating environmental costs, the energy efficiencies of each diet are calculated by decomposing each of the diets into their respective components. Then, the dietary efficiencies are translated into CO₂ emissions. As a first step in estimating health costs, a pooled cross-section time-series dataset is used to find the association between BMI and five countries, …

Boundary Value Problems Of Nabla Fractional Difference Equations, Abigail M. Brackins

Department of Mathematics: Dissertations, Theses, and Student Research

In this dissertation we develop the theory of the nabla fractional self-adjoint difference equation,

∇_a^ν(p∇y)(t)+q(t)y(ρ(t)) = f(t),

where 0 < ν < 1.We begin with an introduction to the nabla fractional calculus. In the second chapter, we show existence and uniqueness of the solution to a fractional self-adjoint initial value problem. We find a variation of constants formula for this fractional initial value problem, and use the variation of constants formula to derive the Green's function for a related boundary value problem. We study the Green's function and its properties in several settings. For a simplified boundary value problem, we show that the Green's function is nonnegative and we find its maximum and the maximum of its integral. For a boundary value problem with generalized boundary conditions, we find the Green's function and show that it is a generalization of the first Green's function. In the third chapter, we use the Contraction Mapping Theorem to prove existence and uniqueness of a positive solution to a forced self-adjoint fractional difference equation with a finite limit. We explore modifications to the forcing term and modifications to the space of functions in which the solution exists, and we provide examples to demonstrate the use of these theorems.

Advisers: Lynn Erbe and Allan Peterson

The Neural Ring: Using Algebraic Geometry To Analyze Neural Codes, Nora Youngs

Department of Mathematics: Dissertations, Theses, and Student Research

Neurons in the brain represent external stimuli via neural codes. These codes often arise from stimulus-response maps, associating to each neuron a convex receptive field. An important problem confronted by the brain is to infer properties of a represented stimulus space without knowledge of the receptive fields, using only the intrinsic structure of the neural code. How does the brain do this? To address this question, it is important to determine what stimulus space features can - in principle - be extracted from neural codes. This motivates us to define the neural ring and a related neural ideal, algebraic objects …

Disaster Salsa, Linda A. H. Sirois

All NMU Master's Theses

ABSTRACT

DISASTER SALSA

By

Linda A. H. Sirois

Disaster Salsa is an interrelated collage of essays, poems, letters, recipes, and stories that are juxtaposed to tell a narrative of what it is like to be the mother of a child who struggles with mental disorder. Our oldest son has had Obsessive Compulsive Disorder (OCD) all his life; at the age of 11 he was struck with a baseball bat, which resulted in a Traumatic Brain Injury (TBI).

Services that address, diagnose, and treat mental disorders have been abysmal for many years in parts of our country, and ten years ago …

To Soar Above The Apple Grove: Stories, Emily V. Doseck

All NMU Master's Theses

The stories written for this thesis revolve around the idea of lesbian characters finding, and in some cases losing, love in the young adult genre. Each piece differs in time period and setting, but all take the typical ‘tragic’ stereotype of queer fiction and prove that everyone deserves a chance at love. All three pieces also contain elements of magic, ranging from the black magic associated with the witches of Salem to the whimsical magic of fairy tales. First love is a time of discovery and new joy for so many, and queer literature could use more of these positive …

"Why Don't You Speak Ordinary English?" She Said Coldly: Linguistic Rebellion In "The Prussian Officer" And Lady Chatterley's Lover, Nicole R. Koroch

All NMU Master's Theses

Criticism of D. H. Lawrence’s novel, Lady Chatterley’s Lover, tends to focus on issues of sexual awakening, the natural world, and figurations of gender and power. However, little examination has been done on the instances of language as a rejection of authority through the character of Mellors, a returning soldier and a gamekeeper. In the novel, Mellors speaks both a “broad” vernacular as well as “proper” English and this linguistic variance reveals two significant aspects of Mellors’ character. Here, I will implement Jacques Derrida’s theory of differance to show that just as the “a” in differance, as a graphic …

Antidepressant Effects Of The Nts1 Agonist Pd149163 In The Forced Swim Test, Lawrence Carey Iv

All NMU Master's Theses

Neurotensin is a neuropeptide that influences monoaminergic neurotransmission in areas of the brain involved in the pathophysiology of depression. The forced swim test is a commonly used screening model for putative antidepressant medications. Drugs that have antidepressant effects in humans reliably decrease the time animals spend in an immobile posture in the forced swim test without increasing general locomotor activity as measured in an open field test. The present study sought to examine the effects of the neurotensin NTS₁ receptor agonist PD149163 and the tricyclic antidepressant drug imipramine on immobility in the forced swim test and on locomotor activity …

The Effect Of Naltrexone On Nicotine-Induced Conditioned Place Preference In Rats, Jonathan M. Adams

All NMU Master's Theses

Nicotine is the central active ingredient in tobacco. The reinforcing effects of nicotine can be studied in animals through self-administration, conditioned place preference, and other approaches that enable researchers to assess nicotine cessation strategies. One strategy involves the use of opioid receptor antagonists. For instance, naloxone has been shown to reduce place preference for nicotine in rats, and other experimental opioid antagonists have also been shown to affect place preference for nicotine. The present study sought to extend these findings to the opioid antagonist naltrexone, which has long been FDA-approved for the treatment of opioid and alcohol addiction in humans. …

Endophytic Actinomycetes As Potential Agents To Control Common Scab Of Potatoes, Alaxandra A. Goodman

All NMU Master's Theses

Potato marketability and tuber quality can be decreased upon infection by a pathogen, Streptomyces scabies. Current disease control consists of expensive irrigation systems and using hazardous chemicals, which exert limited disease control and are potential hazards to those exposed. Biological control offers a cost effective method for controlling disease. Antibiotic producing Streptomyces have indicated their ability to combat plant diseases. Some Streptomyces species colonize within plant tissues without causing disease symptoms (endophytes). This research sought for the presence of endophytic Steptomyces or closely related species in potato plants that were grown in fields containing pathogen-inhibiting Streptomyces. To establish genus …

Around The Table, Rachel E. Mills

All NMU Master's Theses

ABSTRACT

Around the Table

By

Rachel Elizabeth Mills

This collection of nonfiction essays revolves around concepts of food and home. The essays focus on the universalizing nature of food, both from a personal perspective, and from a diasporic Middle Eastern perspective. In these essays I explore how food unifies and creates communities. The essays range from exploring my own upbringing in rural Upper Michigan, and how food creates bonds within my own family and community, to examining how food creates ties and communities within the Arab diaspora. This collective narrative, in focusing on the communal characteristics surrounding the human need …

Conditions Of Confinement At Sentencing: The Case Of Seriously Disordered Offenders, E. Lea Johnston

Catholic University Law Review

No abstract provided.

Synthesis Of A Drug Delivery Vehicle For Cancer Treatment Utilizing Dna-Functionalized Gold Nanoparticles., Tyler Brann

Electronic Theses and Dissertations

The treatment of cancer with chemotherapeutic agents has made great strides in the last few decades but still introduces major systemic side effects. The potent drugs needed to kill cancer cells often cause irreparable damage to otherwise healthy organs leading to further morbidity and mortality. A therapy with intrinsic selective properties and/or an inducible activation has the potential to change the way cancer can be treated. Gold nanoparticles (GNPs) are biocompatible and chemically versatile tools that can be readily functionalized to serve as molecular vehicles. The ability of these particles to strongly absorb light with wavelengths in the therapeutic window …

Construction And Characterization Of Non-Toxic Bacterial Enterotoxins As Vaccine Adjuvants, Lavanya Vempati

Boise State University Theses and Dissertations

The development of adjuvants that can promote the delivery of purified subunit vaccines by mucosal routes, such as the nose or the mouth, is recognized as a top priority for vaccine research. The bacterial enterotoxins; cholera toxin (CT) and E.coli heat-labile toxin (LTI), have long been recognized as powerful adjuvants with the ability to stimulate specific immune responses to co-administered antigens when delivered to mucosal surfaces. Shiga toxin 1 (ST1) and pertussis toxin (PT) are structurally homologous bacterial toxins secreted by Escherichia coli 0157:H7 and Bordetella pertussis respectively. ST1 and PT also have reported adjuvant activity but it is less …

Development And Testing Of The Home Safety And Beautification Checklist With Mothers Referred For Child Neglect And Substance Abuse, Michelle Pitts

UNLV Theses, Dissertations, Professional Papers, and Capstones

Unintentional injury is the leading cause of death among children in the United States accounting for nearly 40 percent of deaths in this age group. Approximately 50 percent of nonfatal injuries, and 40 percent of fatalities, occur in and around the home. Elevated rates of child injury in the home environment are associated with parental substance abuse and neglectful behaviors. Childhood injuries are preventable, yet practical and cost-effective measures to identify child home safety risks are limited. This study involved a retrospective preliminary examination of the Home Safety and Beautification Checklist (HSBC) in a sample of drug-abusing mothers referred by …

Non-Learning Semantic Analysis For Context Discovery And Sentiment Estimation: Transportation Application, Himanshu Verma

UNLV Theses, Dissertations, Professional Papers, and Capstones

With enormous amount of linguistic data present on web, text analysis has become one of the major fields of interest today. This field includes sentiment analysis, information retrieval, text document classification, knowledge based modeling, content similarity measure, data clustering, words prediction/correction, decision making etc. Managing and processing such data has vital importance. The field being quite broad, our focus is mainly on transportation related social media(Twitter) data extraction, text categorization/classification which can be further sub-divided into concept discovery, word sense disambiguation and sentiment analysis to analyze performance of existing transportation system worldwide. Concept discovery is the method of extracting the …

A Poultry Litter Marker Gene Correlates With Pathogen And Indicator Bacteria Deposition On, Growth In And Transport From Poultry Bedding, Kameswari Sirisha Mantha

Graduate Theses, Dissertations, and Problem Reports (ETD)

Poultry is one of the major contributors of inputs to U.S. environmental waters due to the large number of poultry grown per year in the U.S. and litter disposal practices. Two studies were conducted during this research: (1) to determine the temporal rate of deposition and growth or decay of microorganisms in poultry litter originating from poultry feces and (2) the release and transport of poultry litter associated microorganisms from soiled poultry litter applied as fertilizer through soil columns under simulated rainfall. First, deposition studies were conducted to evaluate the deposition rate of microorganisms on poultry bedding during the growth …

Functional And Genetic Analysis Of Choroid Plexus Development In Zebrafish, Hannah Elizabeth Henson

Theses and Dissertations (ETD)

The choroid plexus (CP) is an epithelial based structure localized within the brain ventricles and functions as the blood-cerebrospinal fluid barrier (BCSFB). Under normal conditions, the CP is responsible for generating the cerebrospinal fluid (CSF) and regulating its components. Abnormal CP function has been associated with neurodegenerative diseases, tumor formation in CP epithelia (CPe), and hydrocephalus. Despite the significant role of the CP in these disorders, little research has been done to characterize its functional properties and genetically dissect the pathways involved in normal CP development and disease. For this study, we have utilized zebrafish, (Danio rerio), as a model …

Alliance For Wild Rockies V. Salazar: Congress Behaving Badly, Edward A. Fitzgerald

Villanova Environmental Law Journal (1991 - )

No abstract provided.

Governing A Global Commons: Sharks In The High Seas, Jared R. Wigginton

Villanova Environmental Law Journal (1991 - )

No abstract provided.

In Your Region August 2014, Department Of Primary Industries And Regional Development, Western Australia

Newsletters

No abstract provided.