Digital Commons Network^™

Transcriptome Profiling Of Spinal Muscular Atrophy Motor Neurons Derived From Mouse Embryonic Stem Cells., Miho Maeda, Ashlee W Harris, Brewster F Kingham, Casey J Lumpkin, Lynn M Opdenaker, Suzanne M Mccahan, Wenlan Wang, Matthew E R Butchbach

Department of Pediatrics Faculty Papers

Proximal spinal muscular atrophy (SMA) is an early onset, autosomal recessive motor neuron disease caused by loss of or mutation in SMN1 (survival motor neuron 1). Despite understanding the genetic basis underlying this disease, it is still not known why motor neurons (MNs) are selectively affected by the loss of the ubiquitously expressed SMN protein. Using a mouse embryonic stem cell (mESC) model for severe SMA, the RNA transcript profiles (transcriptomes) between control and severe SMA (SMN2+/+;mSmn-/-) mESC-derived MNs were compared in this study using massively parallel RNA sequencing (RNA-Seq). The MN differentiation efficiencies between control and severe SMA mESCs …

The Glanville Fritillary Genome Retains An Ancient Karyotype And Reveals Selective Chromosomal Fusions In Lepidoptera, Virpi Ahola, Marian R. Goldsmith, Et Al

Biological Sciences Faculty Publications

Previous studies have reported that chromosome synteny in Lepidoptera has been well conserved, yet the number of haploid chromosomes varies widely from 5 to 223. Here we report the genome (393Mb) of the Glanville fritillary butterfly (Melitaea cinxia; Nymphalidae), a widely recognized model species in metapopulation biology and eco-evolutionary research, which has the putative ancestral karyotype of n¼31. Using a phylogenetic analyses of Nymphalidae and of other Lepidoptera, combined with orthologue-level comparisons of chromosomes, we conclude that the ancestral lepidopteran karyotype has been n¼31 for at least 140My. We show that fusion chromosomes have retained the ancestral chromosome segments and …

Regulatory Adaptations For Delivering Information: The Case Of Confession, Daniel Sznycer, Eric Schniter, John Tooby, Leda Cosmides

ESI Publications

Prior to, or concurrent with, the encoding of concepts into speech, the individual faces decisions about whether, what, when, how, and with whom to communicate. Compared to the existing wealth of linguistic knowledge however, we know little of the mechanisms that govern the delivery and accrual of information. Here we focus on a fundamental issue of communication: The decision whether to deliver information. Specifically, we study spontaneous confession to a victim. Given the costs of social devaluation, offenders are hypothesized to refrain from confessing unless the expected benefits of confession (e.g. enabling the victim to remedially modify their course of …

The Prospector, September 5, 2014, Utep Student Publications

The Prospector

Headline: The Centennial Issue: A Century of Impact

Mobile Technology Publications Research Output As Indexed In Engineering Index: A Scientometric Analysis, Santha Kumar Ramiah Mr

Library Philosophy and Practice (e-journal)

The present study analysis has been taken into account in the filed of mobile technology research output using Engineering Index database for selected period of ten years from 2003 to 2012. This study also explores the trend in authorship pattern and collaborative in mobile technology publications with sample of 1, 44,567 research articles during the period from 2003 to 2012. The findings of the study shows that in the year 2011 was the most productive year with 20318 articles (14.05%) published. Among the top ten country wise authors, the highest numbers of articles (22.83%) were contributed by the authors from …

Some Preliminary Thoughts On The Law Of Neighbors, James C. Smith

Georgia Journal of International & Comparative Law

No abstract provided.

Domestic Solutions To The International Problem Of Water Scarcity: Singapore, A Case Study, Suzanne T. Lewis

Georgia Journal of International & Comparative Law

No abstract provided.

River Of Contention: Scarcity Discourse And Water Competition In Highland Peru, Barbara Lynch

Georgia Journal of International & Comparative Law

No abstract provided.

Fishing Moratoria And Securing Turfs: Creating Opportunities For Future Marine Resource Abundance In The Face Of Scarcity In Western Africa, Anastasia Telesetsky

Georgia Journal of International & Comparative Law

No abstract provided.

International Law In A Time Of Scarcity: An Introduction, Harlan G. Cohen

Georgia Journal of International & Comparative Law

No abstract provided.

Turkey's Article 301: A Legitimate Tool For Maintaining Order Or A Threat To Freedom Of Expression?, Jahnisa Tate

Georgia Journal of International & Comparative Law

No abstract provided.

Turtle Power Down Under The Sea?: Comparative Domestic And International Legal Protection Of Marine Turtles By Australia And The United States, Marjorie Palmer

Georgia Journal of International & Comparative Law

No abstract provided.

The Pacifican September 4, 2014, University Of The Pacific

All Issues - Student Newspaper, The Pacifican, Pacific Weekly

No abstract provided.

Quantitative Factors Proposed To Influence The Prevalence Of Canine Tick-Borne Disease Agents In The United States, Roger W. Stich, Byron L. Blagburn, Dwight D. Bowman, Christopher Carpenter, M. Roberto Cortinas, Sidney A. Ewing, Desmond Foley, Janet E. Foley, Holly Gaff, Graham J. Hickling, R. Ryan Lash, Susan E. Little, Catherine Lund, Robert Lund, Thomas N. Mather, Glen R. Needham, William L. Nicholson, Julia Sharp, Andrea Varela-Stokes, Dongmei Wang

Plant Sciences and Entomology Faculty Publications

The Companion Animal Parasite Council hosted a meeting to identify quantifiable factors that can influence the prevalence of tick-borne disease agents among dogs in North America. This report summarizes the approach used and the factors identified for further analysis with mathematical models of canine exposure to tick-borne pathogens.

September 4, 2014, James Madison University

The Breeze, 2010-2019

No abstract provided.

Curriculum Subcommittee Agenda, September 4, 2014, Utah State University

Educational Policies Committee

Department of Agricultural Systems Technology and Education

• New Course
• Title Change
• Credit Hour, Grade Mode Change
• Title, Prerequisite, Course Description Change

Department of Nutrition, Dietetics and Food Science

• New Course

Department of Plants, Soils and Climate

• Credit Hour Change
• Prerequisite Change

Department of Art and Design

• New Course

Department of Theatre Arts

• Grade Mode Change

Department of Family, Consumer and Human Development

• New Course

Department of Health, Physical Education and Recreation

• Title Change
• Prerequisite Change

Department of Psychology

• Title, Prerequisite, Course Description Change

Department of Teacher Education and Leadership

• Course Description change
• Prerequisite Change

Department of Biological Engineering

• New Course …

Pharmacological And Pre-Clinical Testing Of 5-Nidr As A New Therapeutic Agent Against Brain Cancer, Seol Casey Kim, Anthony Berdis, Jung-Suk Choi

Undergraduate Research Posters 2014

Approximately 4,000 children in the United States are diagnosed annually with a brain tumor. Brain cancers are the deadliest of all pediatric cancers as they have survival rates of less than 20%. Although surgery and radiation therapy are widely used to treat adult patients, chemotherapy is the primary therapeutic option for children. One important chemotherapeutic agent is temozolomide, an alkylating agent that causes cell death by damaging DNA. In this project, we tested the ability of a specific non-natural nucleoside developed in our lab, designated 5-NIdR, to increase the efficacy of temozolomide against brain cancer. Cell-based studies demonstrate that the …

Fair Wind Sailing Inc V. H. Dempster

2014 Decisions

USDC for the District Court of the Virgin Islands

The Tiger Vol. 108 Issue 19 2014-09-04, Clemson University

Tiger Newspapers

No abstract provided.

Enhanced Expression Of Codon Optimized Mycobacterium Avium Subsp. Paratuberculosis Antigens In Lactobacillus Salivarius, Christopher D. Johnston, John P. Bannatine, Rodney Govender, Lorraine Endersen, Daniel Pletzer, Helge Weingart, Aidan Coffey, Jim O'Mahony, Roy D. Sleator

Department of Biological Sciences Publications

It is well documented that open reading frames containing high GC content show poor expression in A+T rich hosts. Specifically, G+C-rich codon usage is a limiting factor in heterologous expression of Mycobacterium avium subsp. paratuberculosis (MAP) proteins using Lactobacillus salivarius. However, re-engineering opening reading frames through synonymous substitutions can offset codon bias and greatly enhance MAP protein production in this host. In this report, we demonstrate that codon-usage manipulation of MAP2121c can enhance the heterologous expression of the major membrane protein (MMP), analogous to the form in which it is produced natively by MAP bacilli. When heterologously over-expressed, antigenic determinants …

Lin28a Expression Reduces Sickling Of Cultured Human Erythrocytes, Jaira F. De Vasconcellos, Ross M. Fasano, Y. Terry Lee, Megha Kaushal, Colleen Burns, Emily Riehm Meier, Molly Anderson, Antoinette Rabel, Raul Braylan, David F. Stroncek, Jeffery L. Miller

Pediatrics Faculty Publications

Induction of fetal hemoglobin (HbF) has therapeutic importance for patients with sickle cell disease (SCD) and the beta-thalassemias. It was recently reported that increased expression of LIN28 proteins or decreased expression of its target let-7 miRNAs enhances HbF levels in cultured primary human erythroblasts from adult healthy donors. Here LIN28A effects were studied further using erythrocytes cultured from peripheral blood progenitor cells of pediatric subjects with SCD. Transgenic expression of LIN28A was accomplished by lentiviral transduction in CD34(+) sickle cells cultivated ex vivo in serum-free medium. LIN28A over-expression (LIN28A-OE) increased HbF, reduced beta (sickle)-globin, and strongly suppressed …

The Utah Statesman, September 4, 2014, Utah State University

The Utah Statesman

Weekly student newspaper of Utah State University in Logan.

Ethanol Pharmacokinetics In Neonates And Infants, Elizabeth Marek, Pharmd., Walter K. Kraft, Md

Department of Pharmacology and Experimental Therapeutics Faculty Papers

Introduction:

Ethanol has been used for years in neonatal and infant liquid medications, yet the pharmacokinetics, pharmacodynamics, and safety of ethanol in this vulnerable population have not been well characterized. The purpose of this review is to raise awareness of ethanol use as an excipient in neonatal and infant medications and to provide insight, based on the available evidence, into clearance rates of ethanol in babies. We also discuss ethanol pharmacokinetics in adults, theoretical pharmaco-kinetic changes in neonates and infants as it may apply to ethanol disposition, and case reports involving ethanol exposure in neonates and infants.

Materials and methods: …

The Daily Gamecock, Thursday, September 4, 2014, University Of South Carolina, Office Of Student Media

September

No abstract provided.

Transcriptional Profiles Of Bovine In Vivo Pre-Implantation Development, Zongliang Jiang, Jiangwen Sun, Hong Dong, Oscar Luo, Xinbao Zheng, Craig Obergfell, Yong Tang, Jinbo Bi, Rachel O'Neill, Yijun Ruan, Jingbo Chen, Xiuchun Tian

Faculty Publications

© 2014 Jiang et al.; licensee BioMed Central Ltd. Background: During mammalian pre-implantation embryonic development dramatic and orchestrated changes occur in gene transcription. The identification of the complete changes has not been possible until the development of the Next Generation Sequencing Technology.Results: Here we report comprehensive transcriptome dynamics of single matured bovine oocytes and pre-implantation embryos developed in vivo. Surprisingly, more than half of the estimated 22,000 bovine genes, 11,488 to 12,729 involved in more than 100 pathways, is expressed in oocytes and early embryos. Despite the similarity in the total numbers of genes expressed across stages, the nature of …

The Cowl - V.79 - N.1 - Sep 4, 2014

The Cowl

The Cowl - student newspaper of Providence College. Vol 79 - No. 1 - September 4, 2014. 24 pages.

Histone Methyltransferase Mmset/Nsd2 Alters Ezh2 Binding And Reprograms The Myeloma Epigenome Through Global And Focal Changes In H3k36 And H3k27 Methylation, Relja Popovic, Eva Martinez-Garcia, Eugenia G. Giannopoulou, Quanwei Zhang, Qingyang Zhang, Teresa Ezponda, Mrinal Y. Shah, Yupeng Zheng, Christine M. Will, Eliza C. Small, Youjia Hua, Marinka Bulic, Yanwen Jiang, Matteo Carrara, Raffaele A. Calogero, William L. Kath, Neil L. Kelleher, Ji-Ping Wang, Olivier Elemento, Jonathan D. Licht

Publications and Research

Overexpression of the histone methyltransferase MMSET in t(4;14)+ multiple myeloma patients is believed to be the driving factor in the pathogenesis of this subtype of myeloma. MMSET catalyzes dimethylation of lysine 36 on histone H3 (H3K36me2), and its overexpression causes a global increase in H3K36me2, redistributing this mark in a broad, elevated level across the genome. Here, we demonstrate that an increased level of MMSET also induces a global reduction of lysine 27 trimethylation on histone H3 (H3K27me3). Despite the net decrease in H3K27 methylation, specific genomic loci exhibit enhanced recruitment of the EZH2 histone methyltransferase and become hypermethylated on …

The George-Anne, Georgia Southern University

The George-Anne

• Opinions
• News
• Arts & Entertainment
• Classifieds & Puzzles
• Sports

The Effect Of A Small Ruminant Farm Operation And Sustainable Farm Practices: Soil Quality And Run-Off At The University Hickory Hill Farm, Delaware, Gulnihal Ozbay Dr., Akida J. Ferguson, Raju Khatiwada, Lathadevi K. Chintapenta

Professional Agricultural Workers Journal

This project was designed to evaluate the effect of ruminant grazing practices at Hickory Hill Farm on the surrounding environment by measuring soil nutrients and runoff chemistry. Three pastures on the farm (Goat, Cattle and Control) were selected for soil sampling and nutrient analyses were recorded. Physical water quality parameters were conducted on the runoff collected from the farm after Hurricane Sandy. The sites with animal activity had higher levels of sulfate, phosphate, nitrate, Mehlich 3 phosphorus and conductivity when compared to control site. However, the control site had slightly higher pH and chloride levels. Nitrogen and phosphorous levels were …

Organic Growers Of Alabama Cooperative, A Loosely Fitted Cooperative: Nurturing The Community And Growing Together, Wylin D. Wilson, Jose Gbadamosi, Decetti Taylor, Susan Barnes, Jan Garrett, Asabi Hunter, Cheryl Parker, Wendy Williams, Henry Williams

Professional Agricultural Workers Journal

The age-old connection between the people and the land is something that seven women, who have organized themselves into a loosely fitted cooperative, are reviving. In the process of growing together as members of a cooperative, these women in Macon County, Alabama, are not only promoting healthy living and nurturing community, but are also addressing the issue of food security by making nutritious affordable produce and other agricultural products available to their community. Additionally, they are illustrating alternative strategies of community and economic development. This article examines why they chose the structure of a loosely fitted cooperative over a traditional …