Digital Commons Network^™

Managing Devil Facial Tumour Disease In Tasmanian Devils (Sarcophilus Harrisii): An Investigation Of Heat Shock Proteins As Potential Vaccine Adjuvants, Monika Payerhin

Independent Study Project (ISP) Collection

The world’s largest carnivorous marsupial, the Tasmanian devil (Sarcophilus harrisii), is facing extinction from a deadly, highly communicable cancer that has already decimated over 85% of devil populations in the wild: devil facial tumour disease (DFTD). DFTD cells effectively evade recognition by the immune system, and every devil that contracts the disease dies from it. Many attempts have been made at developing a vaccine that could help save this now-threatened species. Heat shock proteins have been linked to enhanced immune recognition of pathogens, making them potential candidates for acting as adjuvants to such a vaccine against DFTD. In this study, …

The End Of An Era: The Cancer Stem Cell Hypothesis And Its Therapeutic Implications, Emily Paton

Independent Study Project (ISP) Collection

Cancer, a leading cause of death in many developed countries, has become a global health burden. Much of the drain on healthcare systems has resulted from the high cost and ineffectiveness of conventional cancer treatments, which often lead to cancer relapse or metastasis. The failures of chemotherapy, radiation, and surgery have been elucidated by the cancer stem cell (CSC) hypothesis, which postulates that a small percentage of tumor cells drive tumorigenesis. These cells can sustain cancer through their capacity to self-renew and differentiate into specialized tumor cell types, similar to how healthy stem cells maintain healthy tissues. Conventional cancer treatments …

New Study Of Altitude And Butterfly Diversity Evaluation Of Butterfly Diversity In La Hesperia And Influence Of Altitude On Diversity, Savannah Artusi

Independent Study Project (ISP) Collection

Due to the increasing effects of climate change, studies focusing on bioindicator species are becoming more necessary than ever. Additionally, knowledge about global biodiversity can be very useful to conservation organizations because it helps them determine what areas need to be conserved the most .Butterflies are useful as bioindicators due to their complex life cycles, importance in the food chain, and sensitivity to environmental changes. This project focused on studying the overall biodiversity of butterflies in the La Hesperia reserve. In addition, this study aimed to determine how butterfly diversity varies at different altitudes. To do this, the reserve was …

Violence And Disruptive Behavior On The Difficult Trail To Utah, 1847–1868, David L. Clark

BYU Studies

One aspect of the Mormon pioneer experience has not been studied: violence. Did the pioneers have problems with fighting and other aggressive behavior? How did company leaders prevent problems and handle disputes when they inevitably arose? This article reports pioneer records mentioning arguments, punishments, and other violent actions. The stories range from a threatened whipping for children who dallied behind their group to the heartbreaking tale of handcart pioneers being whipped to keep them moving along a frozen trail. While sickness, hunger, accidents, and weather took their toll on the Mormon pioneers, violence was not a major issue. Although records …

Creation As An Ecumenical Problem: Renewed Belief Through Green Experience, Thomas Hughson

Theology Faculty Research and Publications

Loss of a sense of creaturehood and of members has occurred across the lines of divided churches in a secular context. The author explores the question whether green experience of nature can be a path toward a renewed sense of creaturehood. Bernard Lonergan’s distinction between faith and belief allows for identifying a primordial faith that interprets the cosmos as numinous. Ignatius of Loyola’s Spiritual Exercises interprets primordial faith with the biblical word of God as Creator. Why not develop local ecumenical experiments in reevangelization that address green experience?

Virginia Accomplishments Since The 2008 Climate Action Plan Release, Center For Coastal Resources Management, Virginia Institute Of Marine Science, Governor's Climate Change And Resiliency Update Commission

2014, Adaptive Planning for Flooding and Coastal Change in Virginia: Next Steps for the Commonwealth

No abstract provided.

Codex Sinaiticus As A Window Into Early Christian Worship, Timothy N. Mitchell

Liberty Theological Review

Codex Sinaiticus is the oldest and most complete New Testament in Greek known to exist. Its two colophons at the end of 2 Esdras and Esther indicate a possible connection with Pamphilus’ famous library at Caesarea in Palestine. Origen was head of a school for catechumens during his days in Alexandria in Egypt and later began a similar school in Caesarea. Pamphilus was Origen’s star pupil and later directed his school in Caesarea. These colophons may connect Sinaiticus with an ancient tradition of early Christian worship and instruction of new converts, possibly exhibited in particular scribal features. These scribal features …

Exploring A Statistical Test For Modality, James E. Petkus

Graduate Research Theses & Dissertations

This thesis explores the possibility of testing the statistical significance of modality based on the smoothed bootstrap test for modality. There is little information available on the plausibility of Silverman's test, potentially due to the computational requirements essential to perform the required bootstrap testing. Using the statistical package R, and incorporating parallel processing to perform large bootstrap simulations, the goal is to determine if a statistical test to estimate modality based on significance may exist. Simulation studies reveal properties of critical bandwidth and show modifications to the smoothed bootstrap test for modality make it possible to perform a statistical test …

Experimental Validation Of A New Distributed Cooperative Control Algorithm For Multi-Agent Systems With Switching Communication Topologies And Time-Delays, Junzhen Shao

Doctoral Dissertations and Master's Theses

In this thesis, we present practical experimental results to demonstrate a control law for consensus of multiagent systems with switching topologies and time delays. The nonlinear control law utilizes discontinuous cooperative control gains and uses contraction mapping to achieve consensus of multiagent systems. The testing platform we used consists of a number of mobile robots and software simulations both in Matlab and Microsoft Studio C++. We present the effectiveness of the control law design by Aria mobile robots with applications in distributed cooperative formation control. Computer simulations and hardware experiments presented include point consensus control and formation control, both with …

Cloning And Expression Of A Tobacco Stearoyl-Acp Desaturase Gene Sbip24 And Its Interaction With Sabp2 In Sa Pathway, Amin J. Ferdous

Electronic Theses and Dissertations

Salicylic acid binding protein 2 (SABP2) that converts methyl salicylate to salicylic acid (SA) plays an obligatory role in the SA-mediated disease resistance pathway in plants. SABP2 interacts with SBIP24 in a yeast two-hybrid screening. SBIP24 belongs to the stearoyl-acyl carrier protein-desaturase protein family. To biochemically characterize the SBIP24, it was cloned from tobacco leaves using RT-PCR and expressed in E. coli. Recombinant SBIP24 was affinity purified using Ni-NTA chromatography. RT-PCR was performed to determine the role of SABP2 in modulating the expression SBIP24. TMV infected transgenic C3 (control tobacco plant containing empty silencing vector) and 1-2 (SABP2-silenced) …

Impulsivity, Venturesomeness, And Pride: Potential Moderators Of The Relationship Between Childhood Trauma, Substance Use, And Physical Aggression, Joshua P. Hatfield

Electronic Theses and Dissertations

Impulsivity, venturesomeness, and pride variables were examined as potential moderators of the associations between childhood trauma and physical aggression, alcohol use and physical aggression, and drug use and physical aggression. Participants (n = 457) were college students recruited from a university in the Southeast. It was hypothesized that childhood trauma, alcohol use, and drug use would be associated with increased scores of physical aggression. In addition, it was hypothesized that impulsivity, venturesomeness, authentic pride, and hubristic pride would moderate these relationships. Linear, multivariate hierarchical regression analyses were used to examine these variables as potential moderators. Hypotheses concerning hubristic pride as …

Brain Based Learning: K-12 Teachers’ Preferred Methods Of Science Instruction, Donna L. Mansy

Electronic Theses and Dissertations

The purpose of this quantitative study was to investigate Brain Based Learning (BBL) techniques in teaching science. Participants included 216 K-12, full-time, regular education teachers from 8 Northeast Tennessee school systems who taught at least 1 science class. Specifically this research was guided by 7 research questions on teachers’ perceptions and practices in teaching science.

Data were collected by a survey that consisted of 82 statements where teachers rated their level of agreement and was distributed online via Survey Monkey. The first portion of my survey included demographic identifiers, teachers’ knowledge of the term BBL, and inquiries regarding science background …

Cellular-Based Brain Pathology In The Anterior Cingulate Cortex Of Males With Autism Spectrum Disorder, Jessica D. Crawford

Electronic Theses and Dissertations

Autism spectrum disorder (ASD) now affects 1 in 68 children in the United States. Disorders within this spectrum share hallmark deficits in verbal and nonverbal communication, repetitive behavior, and social interaction. The cause of ASD is still unknown. Even though hundreds of genetic abnormalities have been identified in ASD, these markers account for less than 1% of all ASD cases. Researchers continue to search for pathological markers common to all or most cases of ASD. The research presented in this dissertation used a novel combination of state-of-the-art methods to investigate brain pathology in ASD. Postmortem anterior cingulate cortex (ACC) from …

Hif-1Α In The Heart: Provision Of Ischemic Cardioprotection And Remodeling Of Nucleotide Metabolism, Joe Wu

Electronic Theses and Dissertations

In our studies we found that stabilized expression of HIF-1α in heart led to better recovery of function and less tissue death after 30 minutes of global ischemia, via mechanisms that preserve the mitochondrial polarization. Our group previously showed that HIF-1α conferred ischemic tolerance by allowing cardiomyocytes to use fumarate as an alternative terminal electron acceptor to sustain anaerobic mitochondrial polarization. The source of fumarate was identified as the purine nucleotide cycle (PNC). Here we discovered that HIF-1α upregulates AMP deaminase 2 (AMPD2), the entry point to the PNC. The combination of glycolysis and the PNC may protect the heart's …

A Comprehensive Study Of The Effects Of Neurotoxins On Noradrenergic Phenotypes, Neuronal Responses And Potential Intervention By Antidepressants In Noradrenergic Cells, Yan Wang

Electronic Theses and Dissertations

It has been reported that locus coeruleus (LC) degeneration precedes the degeneration of other neurons in the brain in some neurodegenerative diseases like Alzheimer’s disease (AD) and Parkinson’s disease (PD). However, the precise mechanisms of neurodegeneration remain to be elucidated. N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine (DSP4) has been widely used as a noradrenergic neurotoxin in the development of AD and PD animal models for specific LC degeneration. However, the precise mechanism of action of DSP4 remains unclear. An increased systemic DNA damage caused by neurotoxin or oxidative stress has been found to be related to the pathogenic development of neurodegeneration. The process of neurodegeneration …

Identification, Cloning, And Expression Of Tobacco Responsive To Dehydration Like Protein (Rd22), Sbip-355 And Its Role In Sabp2 Mediated Sa Pathway In Plant Defense, Hanadi Abdulaali Almazroue

Electronic Theses and Dissertations

Abscisic acid (ABA) induces RD22, responsive to dehydration stress gene. Salicylic acid (SA) has been the focus of research due to its role against pathogens and abiotic stress. Interaction between ABA and SA signaling pathways is still poorly understood. SA-Binding Protein 2 (SABP2) converts methyl salicylate to SA. An attempt was made to identify proteins that interact with SABP2 using a yeast 2-hybrid screening. Several interactors were identified. One of them, SA-Binding Protein 2 Interacting Protein-355 (SBIP-355), showed high homology to RD22. Bioinformatic approaches showed that SBIP-355 contains a BURP domain. Phylogenetic analysis reveals that SBIP-355 clustered into the …

The Wolf Attacks: A History Of The Russo-Chechen Conflict, Christina E. Baxter

Electronic Theses and Dissertations

In the 1990s and early 2000s, the Chechens fought against the Russians for independence. The focus in the literature available has been on the wars and the atrocities caused by the wars. The literature then hypothesizes that the insurgency of today is just a continuation of the past. They do not focus on a major event in Chechen history: the Soviet liquidation of the Chechen-Ingush Autonomous Soviet Socialist Republic in 1944. It is this author’s assertion that the liquidation of the Chechen-Ingush ASSR forever changed the mindset of the people because it fractured a society that was once unified. This …

Functional Characterization Of The Arginine Vasotocin 4 Receptor (Vt4r) In Sensory Circumventricular Organs Of The Chicken Gallus Gallus, Nguiessan Alphonse Aman

Graduate Theses and Dissertations

Past studies have shown that the avian vasotocin 4 receptor (VT4R), homologous to the mammalian arginine vasopressin receptor 1a (V1aR/AVPR1A) is involved in immobilization stress. It was not known, however, whether the receptor is also associated with osmotic stress, and if so, what brain regions may be involved. Four treatment groups of chicks were used for the study. One group was subjected to 1h immobilization stress and two groups were administered intraperitoneal injection of 3 M NaCl or 0.15 M NaCl. One additional group served as controls. After 1 h, blood samples were taken for the determination of plasma levels …

Redesign Of Trans-Splicing Molecules For The Correction Of Dystrophia Myotonica Type 1 Toxic Rna Transcripts, Eleanor G. Harrison

Undergraduate Honors Theses

Dystrophia myotonica (DM1), one of the most common forms of muscular dystrophy, is caused by a repeated trinucleotide expansion in the DMPK gene. This mutation results in the accumulation of toxic cellular RNA transcripts. Spliceosome-mediated RNA trans-splicing (SMaRT) technology is a form of gene therapy that possesses the potential to correct these toxic RNA transcripts and thus cure the disease. Despite its promise, prior research applications of SMaRT technology to DM1 have been hampered by poor efficiency and have not been validated in a relevant model of the disease. In order to improve the efficiency of trans-splicing, this study examined …

Was Gawain A Gamer?, Gus Forester

Undergraduate Honors Theses

Describes a theory of gaming inspired primarily by Jean Baudrillard’s claim that gaming is characterized by a “passion for rules.” Key elements of the theory include that games are an attempt to create a new reality, that games create a space for individuality even in an otherwise homogenized world, and that pain and happiness are not diametrically opposed concepts to the gamer. The theory also emphasizes the importance of the player’s meeting with the “superplayer,” the player’s own constructed ideal that he tries to imitate within the game world. This theory of gaming is then applied to the 14^{th …}

"Beyond The Pavement" And "Setting Fire To The Sky" With Critical Introduction: "Exploring The Dark: Gothic Short Stories", Samantha Nicole Campbell

Undergraduate Honors Theses

This thesis explores the genre of gothic literature by outlining the themes and common techniques that writers use. It discusses prominent writers in the genre, as well as critiques their techniques and compares them to my own. Two fiction pieces are accompanied with the critical introduction that fit the gothic literature genre.

Law’S Evolution And Law As Custom, William A. Edmundson

San Diego Law Review

normative, and law works by channeling custom-in-gross into progressively finer and more precise grooves. If there is normative moral value resident in the custom of elevating and following leaders, then that normativity ought to flow downstream into the finer channels officials carve and into the fresh territory they wish us to occupy. In places, that flow is too diluted, and normativity trails off. In places, officials direct the stream over a cliff, and it is no longer normative at all. In places, the stream is overtaken by stronger normative streams and can only make a difference yet farther downslope, where …

Do People Obey The Law?, Frederick Schauer

San Diego Law Review

It is customary in a symposium honoring a book as valuable as Laurence Claus’s for the commentators to begin by noting their general agreement with the author’s thesis and then explaining that, in the spirit of academic engagement, they will focus on one small but interesting area in which the author and the commentator disagree. On this occasion, however, it seems more appropriate to reverse that approach. For reasons I will make clear, I am in substantial disagreement with Claus’s normative argument against authority. Unlike Claus, I believe that “because I said so” is often, especially when backed by the …

Introduction For The Symposium, Steven D. Smith, Larry Alexander

San Diego Law Review

“Congress shall make no law respecting an establishment of religion, or prohibiting the free exercise thereof . . .” These first words of the First Amendment would seem to designate religion as a special category in constitutional law. While acknowledging this textual designation, however, some legal scholars have come to view the category as a product of historical contingencies of the late eighteenth-century; they have doubted whether persuasive contemporary justifications are available for giving special constitutional treatment to religion. (Or at least special favorable treatment: scholars have tended to be more approving of special constitutional burdens or disabilities imposed on …

Where's The Beef?, Stanley Fish

San Diego Law Review

A key concern of the papers written for this conference is the relationship between religious beliefs and secular beliefs of the kind that carry with them deep ethical obligations. Are these systems of belief essentially the same or are they different in important respects? The question is typically posed abstractly, and I thought it might be useful to have before us an example of religious belief and the demands that attend it. The example is taken from the beginning of John Bunyan’s The Pilgrim’s Progress. Christian, Bunyan’s protagonist, has suddenly become aware that his salvation is imperiled, and he is …

Religion As A Legal Proxy, Micah Schwartzman

San Diego Law Review

In what follows, after briefly summarizing Koppelman’s position, I argue that his view is vulnerable to the charge that using religion as a legal proxy is unfair to those with comparable, but otherwise secular, ethical and moral convictions. Koppelman has, of course, anticipated this objection, but his responses are either ambivalent or insufficient to overcome it. The case for adopting religion as a proxy turns partly on arguments against other potential candidates. In particular, Koppelman rejects the freedom of conscience as a possible substitute. But even if he is right that its coverage is not fully extensive with the category …

How Much Autonomy Do You Want?, Maimon Schwarzschild

San Diego Law Review

At root, the questions of special accommodation and religious adjudicatory independence arise most urgently when a government grows in its reach and ambition. After all, if most areas of life, including those that touch on religious life, are left to people’s private arrangement, then not much special accommodation will be necessary. But when government takes control over more and more areas of life, regulating who shall do what and under what rules and conditions, then clashes with one or another religious way of life are almost inevitable. The dispute over government mandates to provide abortive drugs and contraception, in the …

Behavioral Assessment Of Acute Inhibition Of System XC - In Rats, Victoria Lutgen, Jon M. Resch, Krista Qualmann, Nicholas J. Raddatz, Cristina Panhans, Ellen M. Olander, Linghai Kong, Sujean Choi, John R. Mantsch, David A. Baker

Biomedical Sciences Faculty Research and Publications

Rationale

Gaps in our understanding of glutamatergic signaling may be key obstacles in accurately modeling complex CNS diseases. System x_c ^- is an example of a poorly understood component of glutamate homeostasis that has the potential to contribute to CNS diseases.

Objectives

This study aims to determine whether system x_c ^- contributes to behaviors used to model features of CNS disease states.

Methods

In situ hybridization was used to map mRNA expression of xCT throughout the brain. Microdialysis in the prefrontal cortex was used to sample extracellular glutamate levels; HPLC was used to measure extracellular glutamate and tissue …

Solar Brine Concentrator, Aric Gregory Fisher

Chemical Engineering Undergraduate Honors Theses

In arid regions of the United States potable water is a valuable resource and is sometimes produced using energy intensive processes by removing salts from brackish water. Task 4 provides a solar option for producing potable water from brackish water; in competing in Task 4, H2OGS has developed a simple, cost effective solar desalination process to provide these regions with necessary, potable water. H2OGS’ design takes into account WERC requirements, including cost, energy usage, worker safety, and easily obtained materials. U.S. government regulatory requirements are also taken into account as the proposed location for this process is Alamogordo, New Mexico. …

Werc: Solar Brine Concentrator, Sarah K. Haley

Chemical Engineering Undergraduate Honors Theses

In arid regions of the United States potable water is a valuable resource and is sometimes produced using energy intensive processes by removing salts from brackish water. Task 4 provides a solar option for producing potable water from brackish water; in competing in Task 4, H2OGS has developed a simple, cost effective solar desalination process to provide these regions with necessary, potable water. H2OGS’ design takes into account WERC requirements, including cost, energy usage, worker safety, and easily obtained materials. U.S. government regulatory requirements are also taken into account as the proposed location for this process is Alamogordo, New Mexico. …