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Basis For Targeting Met Activation Mediated Resistance To Pi3k Inhibition In Breast Cancer, Ana M. Gonzalez-Angulo

Dissertations and Theses (Open Access)

The identification of resistance mechanisms to emerging therapies, such as those targeting the PI3K pathway and the MET receptor, has the potential to benefit a significant number of patients with breast cancer. In this study we hypothesized that concurrent aberrations in PI3K and MET will render breast cancers resistant to therapies targeting each pathway, and that combination therapy targeting the PI3K and MET pathway will optimize therapy-effect by preventing the acquisition of resistance.

We analyzed cMET and phospho-cMET levels in 257 breast cancer samples and found that high levels of both the proteins were seen in all breast cancer subtypes, …

Characterization Of The Rna Binding And Rna Degrading Subunits Of The Eukaryotic Exosome, Borislava Tsanova

Dissertations and Theses (Open Access)

The exosome is an essential complex of ten proteins involved in the processing and degradation of many RNAs in the cell. These include various stable RNAs, mRNAs, and aberrant transcripts both in the nucleus and in the cytoplasm.

In this work I characterize the three members of the exosome “cap”, the RNA binding proteins Rrp4, Rrp40, and Csl4. I determine that in spite of their structural similarity, they each have a unique essential role. Second, I determine that two of the cap proteins Rrp4 and Rrp40 have a role in bridging subunits of the PH ring of the exosome. The …

Neural Correlates Of Audiovisual Speech Perception In Aphasia And Healthy Aging, Sarah H. Baum

Dissertations and Theses (Open Access)

Understanding speech in face-to-face conversation utilizes the integration of multiple pieces of information, most importantly the auditory vocal sounds and visual lip movements. Prior studies of the neural underpinnings of audiovisual integration in the brain have provided converging evidence to suggest that neurons within the left superior temporal sulcus (STS) provide a critical neural hub for the integration of auditory and visual information in speech. While most studies of audiovisual processing focus on neural mechanisms within healthy, young adults, we currently know very little about how changes to the brain can affect audiovisual integration in speech. To examine this further, …

Scanless Fast Handoff Technique Based On Global Path Cache For Wlans, Weetit Wanalertlak, Ben Lee, Chansu Yu

Electrical and Computer Engineering Faculty Publications

Wireless LANs (WLANs) have been widely adopted and are more convenient as they are inter-connected as wireless campus networks and wireless mesh networks. However, timesensitive multimedia applications, which have become more popular, could suffer from long end-to-end latency in WLANs.This is due mainly to handoff delay, which in turn is caused by channel scanning. This paper proposes a technique called Global Path-Cache (GPC) that provides fast handoffs in WLANs.GPC properly captures the dynamic behavior of the network andMSs, and provides accurate next AP predictions to minimize the handoff latency. Moreover, the handoff frequencies are treated as time-series data, thus GPC …

Less Acting, More Doing: How Surface Acting Relates To Perceived Meeting Effectiveness And Other Employee Outcomes, Linda R. Shanock, Joseph A. Allen, Alexandra M. Dunn, Benjamin E. Baran, Cliff W. Scott, Steven G. Rogelberg

Psychology Faculty Publications

This study adds to the growing body of research on work meetings and extends the emotional labour literature beyond a service context by examining the relationship between surface acting during meetings and perceived meeting effectiveness. Additionally, the relationships of surface acting during meetings and perceived meeting effectiveness with time-lagged reports of intention to quit and emotional exhaustion 3 months later were investigated. Structural equation modelling of data from 178 working adults revealed negative relationships between surface acting and perceptions of meeting effectiveness. Perceived meeting effectiveness partially mediated the relationship between surface acting and both intention to quit and emotional exhaustion …

Regulation Of The Myostatin Protein In Overload-Induced Hypertrophied Rat Skeletal Muscle, Paige Abriel Affleck

Theses and Dissertations

Myostatin (GDF-8) is the chief chalone in skeletal muscle and negatively controls adult skeletal muscle growth. The role of myostatin during overload-induced hypertrophy of adult muscle is unclear. We tested the hypothesis that overloaded adult rodent skeletal muscle would result in reduced myostatin protein levels. Overload-induced hypertrophy was accomplished by unilateral tenotomy of the gastrocnemius tendon in male adult Sprague-Dawley rats followed by a two-week period of compensatory overload of the plantaris and soleus muscles. Western blot analysis was performed to evaluate changes in active, latent and precursor myostatin protein levels. Significant hypertrophy was noted in the plantaris (494 ± …

Shaping Swarms Through Coordinated Mediation, Shin-Young Jung

Theses and Dissertations

A swarm is a group of uninformed individuals that exhibit collective behaviors. Without any information about the external world, a swarm has limited ability to achieve complex goals. Prior work on human-swarm interaction methods allow a human to influence these uninformed individuals through either leadership or predation as informed agents that directly interact with humans. These methods of influence have two main limitations: (1) although leaders sustain influence over nominal agents for a long period of time, they tend to cause all collective structures to turn in to flocks (negating the benefit of other swarm formations) and (2) predators tend …

Tcap Scores And Per Pupil Expenditures: Statewide Changes Before And After Tennessee’S First To The Top Act, Martha Ely Cantrell

Electronic Theses and Dissertations

The purpose of this study was to investigate the relationships between the changes in Tennessee Comprehensive Assessment Program (TCAP) scores and the changes in Per Pupil Expenditures (PPE) after the enactment of First to the Top Act of 2010 and the receipt of $501,000,000 in federal Race to the Top (RTTT) grant monies. Half of that money was retained by Tennessee Department of Education (TDOE) for education reform initiatives. The other half was awarded to each Local Education Agency (LEA) according to the Title I formula after TDOE approval of individual Scopes of Work. Reform initiatives included transition to Common …

Mathematics Curriculum Coaching And Elementary School Students’ Mathematics Achievement In A Northeast Tennessee School System, Evandro R. Valente

Electronic Theses and Dissertations

Educators and policymakers have demonstrated interest in finding ways to better equip mathematics teachers so they can help students achieve at a higher level. Academic coaching has been identified as an effective professional development activity for teachers. The purpose of this study was to investigate the difference between students’ achievement levels before and after a mathematics initiative in a Northeast Tennessee school district. In this study I analyzed grades 3 – 6 students’ Tennessee Comprehensive Assessment Program or TCAP scores in the year prior to the hiring of a mathematics coach and their respective scores 2 years after the placement …

A Study Of The Aiken-Rhett Stew Stove, Julia Anne Tew

All Theses

The stew stove found in the kitchen of the Aiken-Rhett House in Charleston, South Carolina is a rare and well-preserved example of antebellum stew stove technology. This masonry stove was installed in the main kitchen of Governor William Aiken in 1858 and contains six cast iron stew holes and a set kettle. Masonry cook stoves appeared in the United States as early as the mid-eighteenth century. Stoves like this were not an American invention. A French device known as the potager is the predecessor and inspiration for such devices. This potager eased the cook's labors in preparing meals and offered …

Knowledge Extraction From Work Instructions Through Text Processing And Analysis, Abhiram Koneru

All Theses

The objective of this thesis is to design, develop and implement an automated approach to support processing of historical assembly data to extract useful knowledge about assembly instructions and time studies to facilitate the development of decision support systems, for a large automotive original equipment manufacturer (OEM). At a conceptual level, this research establishes a framework for sustainable and scalable approach to extract knowledge from big data using techniques from Natural Language Processing (NLP) and Machine Learning (ML). Process sheets are text documents that contain detailed instructions to assemble a portion of the vehicle, specification of parts and tools to …

Characterization Of The Hydrogeology And Stress State In The Vicinity Of The Homestake Mine, Lead, Sd, Johnathan Ebenhack

All Theses

Underground workings in fractured rock are common worldwide. They have applications in numerous areas and fields of study. These include mining operations, civil engineering projects like tunnels and underground facilities, and research projects that require underground laboratories such as the physics research being conducted by Sanford Laboratory at the former Homestake mine and Fermi Laboratory near Chicago (Bahcall et al. 2001, Elsworth 2009, Sadoulet et al. 2006, bge science@DUSEL, fnal.gov). These excavations can reach several kilometers in depth including the 3.9 km deep TauTona mine in South Africa, the 3 km deep LaRonde mine in Quebec and the 2.4 km …

An Examination Of Twitter's Role In The Formation Of Collegiate Sport Fans' Allegiance Using The Revised Psychological Continuum Model (Pcm), Sukjoon Yoon

All Theses

Allegiance has long been considered one of the most notable research topics in sport studies (Funk & Pastore, 2000; Wann & Branscombe 1990). Allegiant fans are those who have formed strong connections to their favorite team, and these attitudes strengthen their psychological involvement. Twitter has grown dramatically since its inception in 2006, totaling more than 500 million users as of early 2013 (Gupta, Goel, Lin, Sharma, Wang, & Zadeh, 2013). Twitter is one of the most popular communication technologies used by both fans and sport organizations (Clavio, 2011), and allows individuals, organizations, and other social groups to connect with one …

Knowledge Base Representation With Axiomatic Design Rules For Concept Level Up Implement In Ontological Framework, Zhuochen Shi

All Theses

Axiomatic Design has been applied and developed as a tool, offering a scientific basis for design and improving design activities. Axiomatic Design has been used in various fields such as software system design, structure design, and product design. However, several challenges and limitations exist in Axiomatic Design including: the inconsistency in identifying design parameters, existence of coupled design, and multiple groups of functional requirements and design parameters. Aimed at using Axiomatic Design to generate conceptual solutions in engineering design while overcoming its limitations, a formal ontology is developed. The ontology defines functional requirements, design parameters, concepts, components and variables and …

The Process Of Passage, David Gerhard

All Theses

My art navigates the fragmented religious traditions of my childhood in an attempt to clarify my thoughts and feelings about which spiritual beliefs and practices I want to teach my children. This navigation is represented across art media through portraits of my family and myself. As I was raised in a multicultural family that combined aspects of Buddhism, Christianity, Hinduism, and Judaism, a complex navigation occurs within me to find meaning in existence amidst disparate traditions, cultures, and beliefs. For my thesis show, I adapted existing fragments and frameworks from these varied beliefs and cultural traditions into a collage of …

Sequential Autotrophic-Heterotrophic Culture Of Scenedesmus Spp. For Lipid Production, Jazmine Taylor

All Theses

Algae are fascinating organisms that are receiving growing attention for their versatility on many fronts of research areas. Immense amounts of research is being conducted to find solutions to challenges in energy and climate change. A large portion of research is focused on algae as a potential solution to both of these issues. The focus of this research is to look at algae closer to home as a local, economical resource to aid in solving both of these problems. The algal genus Scenedesmus was tested for growth in a bi-phased scheme: photoautotrophically for carbon sequestration then heterotrophically on glycerol for …

Scratch Damage Evolution On Metals In Total Joint Replacements, Caleb Eljach

All Theses

The goal of total joint replacement (TJR) is to replace nonfunctioning joint components and relieve pain, improve quality of life, and to improve joint function. Although TJR is a successful procedure, more than 54,000 knee revisions occur each year due to factors, such as wear, loosening, infection, fracture, instability, and patient related factors (Ranawat, 2010). TJRs consist of components typically composed of polyethylene and a metallic counterface (Navarro, 2008). The sliding contact that occurs between the metal and polyethylene components has been well studied and is shown to produce both surface damage and wear of the implant components (Barbour, 1997; …

Dissolved Carbon Dioxide For Scale Removal In Reverse Osmosis, Erin Partlan

All Theses

Membrane fouling is a major operational issue in reverse osmosis (RO) desalination plants. In particular, plants treating brackish groundwater can encounter troublesome inorganic scales, including carbonates, sulfates, and silicates. A novel cleaning method is proposed to remove inorganic scales from fouled RO membranes usinag dissolved CO ₂ . As CO₂ molecules encounter membrane foulants, the surfaces serve as nucleation sites for small bubbles to form and shear off foulants. Dissolved CO₂ solutions were prepared by bubbling CO₂ gas into water held in a pressure vessel. Gas dissolution was confirmed by enhanced exit velocities for water containing CO …

Rendez Donc A Cesar, Problemes Avec Les Mots De Dieu: Land And The Civil Constitution Of The Clergy Of 1790-1791, Jonathan Monroe

All Theses

This study investigates the state's sale of Church lands and the Civil Constitution of the Clergy during the French Revolution. The Civil Constitution has been seen as a turning point in the era's progression; it created very sharp divisions in revolutionary ideals by forcing clergy members to take an oath to the state that was condemned by the pope. These divisions helped feed Jacobin extremism and an era of Christian suppression and the Terror eventually ensued. Despite these problems, the struggling country under the Old Regime was desperate for Church reform that the Civil Constitution provided. The prohibition of the …

Adaptive Transmission For Ofdm, Michael Juang

All Theses

To respond to dynamic channel conditions caused by fading, shadowing, and other time-varying disturbances, orthogonal frequency division multiplexing (OFDM) packet radio systems should adapt transmission parameters on a packet-by-packet basis to maintain or improve performance over the channel. For this to be possible, there are three key ideas that must be addressed: first, how to determine the subchannel conditions; second, which transmission parameters should be adapted; and third, how to adapt those parameters intelligently. In this thesis, we propose a procedure for determining relative subchannel quality without using any traditional channel measurements. Instead, statistics derived solely from subcarrier error counts …

Improvements In The Process Of Biohydrogen Production By Thermotoga Neapolitana, Louis Hill

All Theses

The production of biological hydrogen is an important process for the future of sustainability and alternative energies. Thermotoga neapolitana is a hyperthermophilic bacterium that produces H ₂ , CO₂ and acetate via fermentation. The goal of the research was to investigate the sustainable production of H₂ gas using waste agricultural feedstocks, recycled water and a simplified N₂ sparging method technique. A limited sparging method was developed which includes 1 minute of N₂ gas sparging of the reactor headspace and 90 minutes of idle reaction time for cysteine-HCl to react with dissolved oxygen prior to inoculation. This …

Macroscopic Investigation Of Ionic- And Nano-Silver Toxicity To Soil Nitrification Process, Abdurrahman Masrahi

All Theses

The worldwide production of engineered nanoparticles (ENPs) has grown rapidly in our era because of the popular applications in industrial and consumer markets. Especially silver (Ag) NP is one of mostly common ENPs in our consumer products due to its unique antimicrobial property. However, release of AgNPs into environment through wastewater treatment plants poses a question that what impact AgNPs have on microbially mediated processes in environment (e.g., nutrient and trace element cycles). In the last decades, numerous toxicological studies of AgNPs on bacteria have been conducted in pure culture systems, and several different antimicrobial mechanisms of AgNPs have been …

Evaluating The Predictive Capability Of Numerical Models Considering Robustness To Non-Probabilistic Uncertianty In The Input Parameters, Parker Shields

All Theses

The paradigm of model evaluation is challenged by compensations between various forms of errors and uncertainties that are inherent to the model development process due to, for instance, imprecise model input parameters, scarcity of experimental data and lack of knowledge regarding an accurate mathematical representation of the system. When calibrating model input parameters based on fidelity to experiments, such compensations lead to non-unique solutions. In turn, the existence of non-unique solutions makes the selection and use of one `best' numerical model risky. Therefore, it becomes necessary to evaluate model performance based not only on the fidelity of the predictions to …

The Effect Of Cell Culture Environment On Expression Of Cd44+, Ki67, And Foxo3a And Its Implications On The Role Of Foxo3a In Triple Negative Breast Cancer, Erin Vegter

All Theses

Triple negative breast cancer (TNBC) is a highly aggressive cancer, which is known for a high rate of metastasis and poor prognosis. TNBC cells are hypothesized to undergo the cellular processes epithelial-to-mesenchymal transition (EMT) and mesenchymal-to-epithelial transition (MET). The known prognostic markers CD44+ and Ki67 as well as the transcription factor and proposed tumor suppressor FoxO3a are thought to be indicators of EMT and MET activity in cancer cells. In order to better understand these processes an effective cell culture technique to mimic the tumor environment needs to be established. The purpose of this study was to determine if changing …

Remembering And The Everyday: The Fleeting, Fragmented, And Sensual, Alyssa Prince

All Theses

Our memories are malleable. There is no identifiable truth; we consistently change them based on our present state of being. Nevertheless, our memories influence us greatly. My paintings engage this topic of the remembering process and display the active, reconstructive nature of how we remember. This idea is approached through making paintings that engage my own involuntary memories that are inevitably impossible to grasp. The resulting paintings explore issues pertaining to the remembering process, partiality, and the inaccuracies of representation. In this thesis, I discuss the importance of the reconstructed, provisional image, the fluctuating nature of our memories, an interaction …

Functional Stroke Recovery Through Tissue Engineered Niche Neural Constructs, Natasha Topoluk

All Theses

According to the Centers for Disease Control and Prevention, stroke is statistically responsible for 1 in every 19 deaths of American citizens.5 Stroke is the leading cause of permanent disability due to the fact that it compromises both cellular and tissue components of the brain, leading to the formation of a physical void within the tissue. Current research approaches address the cellular component by injecting stem cells into this void; however, extremely low cell engraftment, high injected cell death, and overall no matrix regeneration are observed. Additionally, these stem cells do not remain committed to a neural lineage. We propose …

A Study Of Gallery Method To Increase Idea Quality In Conceptual Design, Liz Mathew

All Theses

The objective of this research is to evaluate whether gallery method increases idea quality in conceptual design for engineering design problems. The gallery method is a group ideation process which allows individuals to first generate ideas individually, followed by pooling of ideas to conduct group ideation. Design engineers are often encouraged to work in groups to generate concepts and solutions for design problems. However, past research argues that individual ideation results in higher quality design concepts compared to the group ideation methods. The limitations of group ideation such as free riding, production blocking, evaluation apprehension, and goal incongruity are identified …

Bite Detection And Differentiation Using Templates Of Wrist Motion, Soheila Eskandari

All Theses

We introduce a new algorithm of bite detection during an eating activity based on template matching. The algorithm uses a template to model the motion of the wrist over a 6-second window centered on the time when a person takes a bite. We also determine if different types of bites (for example food vs. drink, or using different types of utensils) have different wrist motion templates. This method is implemented on 22,383 bites and 5 different types of templates are built. We then describe a method to recognize different types of bites using the set of templates. The obtained accuracy …

An Exploratory Study On The Effects Of Goal Alignment, Interventions, And Motivational Factors On Innovative Engineering Design Projects, Blake Linnerud

All Theses

Innovation has become an important facet of engineering design, both in industry and the academy. Many senior-level engineering design courses encourage students to develop innovative solutions to open design projects from industry sponsors. Like industry, these academic problems are tackled by teams of students. Student teams that function at the highest level are more likely to reach the innovative solutions for which they are searching. The research presented in this work focuses on two main areas: (1) understanding what motivates engineers when working on innovative design projects and (2) determining the effects of goal alignment interventions on design teams working …

The Effects Of Different Size Gold Nanoparticles On Mechanical Properties Of Vascular Smooth Muscle Cells Under Mechanical Stretching, Tri Kieu

All Theses

The field of nanotechnology research has seen a large growth in the past few decades due to the great potential of novel nano-size material for useful applications such as drug delivery and medical imaging. Also, they are a promising platform for the detection of terrible diseases such as cancer and atherosclerosis. Although these materials hold great promise, there is very limited understanding about the biological effects of these nanoparticles on normal healthy cells, such as vascular smooth muscle cells (VSMCs). The VSMCs are unique cells due to the two distinct phenotype characteristics it can express: a contractile and a synthetic …