Digital Commons Network^™

Nmr Assignments And Structure Of The I-Domain From The Bacteriophage P22 Coat Protein, Alessandro A. Rizzo

Master's Theses

The P22 coat protein has the HK97 fold at its core, but also has an additional domain on the surface of the capsid. Two recent cryo-EM structures at ~8 Å and ~4 Å resolution show different folds for this domain. The structure and function of this domain has been debated in the literature.

NMR spectroscopy was used to determine a high-resolution structure of this domain. Here, the domain is called the ‘insertion’ domain (I-domain). The structure is a six-stranded β-barrel with a small helix and belongs to a reductase/isomerase/elongation factor folding family.

Compared to the previous cryo-EM reconstructions, the NMR …

The Effects Of Time Of Day On Resistance Exercise Workout Responses, Heather N. Husmer

Master's Theses

No abstract provided.

Dynamics Of The P22 Bacteriophage I-Domain By 15n Nmr Relaxation And Native-State Hydrogen Exchange, Latasha Cr Fraser

Master's Theses

The bacteriophage P22 I-domain structure contains a 6-stranded b-barrel folding motif, a 3-stranded b-sheet, and a small a-helical region. This study describes the dynamics of the I-domain determined by ¹⁵N NMR relaxation and native-state hydrogen exchange experiments. Relaxation data suggests that the I-domain is experiencing the majority of its motion on the ps-ns timescale. S² order parameters show two loop regions, which are poorly defined in the NMR structure, are the most flexible regions. The thermodynamic stability of the I-domain to unfolding, as a function of denaturant, has been studied with circular dichroism (CD) and native-state hydrogen exchange …

Teacher-Efficacy For Using Hots Pedagogy In The Classroom, Lindsey K. Le

Master's Theses

When considering the educational context and expectations of our teachers today, an evaluation of teacher-efficacy in promoting higher-order thinking skills (HOTS) in the classroom is more important than ever. Promoting these skills as illustrated in Anderson and Krathwohl’s (2001) taxonomy of educational objectives, through pedagogical techniques, provides an opportunity for students to develop metacognitive skills for learning. The literature (Thomas & Walker, 1997) stresses that the confidence that teachers have in actually promoting these skills (also known as teacher-efficacy (Bandura, 1986)), is important to bridging the gap between pedagogy and development of student thinking skills. The aim of the study …

Rates Of Obesity And Incidence Of Diabetes In Hispanics In The United States: An Examination Of The Epidemiological Paradox, Lucy Dean

Honors Theses

Using pooled data from the 2008-2011 National Health Interview Survey and employing multinomial and binomial logistic regression methods, this research examines disparities in rates of obesity and incidence of diabetes between individual Hispanic subgroups in comparison to non-Hispanic whites and blacks. Immigration status(including nativity, duration in the United States, and citizenship status) is hypothesized to play a central role in rates and obesity and incidence of diabetes. Unlike Cuban-Americans, Mexican-Americans, Puerto Ricans, and other Hispanics were more likely to be overweight as well as obese when compared to non-Hispanic whites. Mexican-Americans had the only significance in prevalence of type 2 …

Discovering Chromatin Motifs Using Faire Sequencing And The Human Diploid Genome, Chia-Chun Yang, Michael J. Buck, Min-Hsuan Chen, Yun-Fan Chen, Hsin-Chi Lan, Jeremy J.W Chen, Chao Cheng, Chun-Chi Liu

Dartmouth Scholarship

Background: Specific chromatin structures are associated with active or inactive gene transcription. The gene regulatory elements are intrinsically dynamic and alternate between inactive and active states through the recruitment of DNA binding proteins, such as chromatin-remodeling proteins. Results: We developed a unique genome-wide method to discover DNA motifs associated with chromatin accessibility using formaldehyde-assisted isolation of regulatory elements with high-throughput sequencing (FAIRE-seq). We aligned the FAIRE-seq reads to the GM12878 diploid genome and subsequently identified differential chromatin-state regions (DCSRs) using heterozygous SNPs. The DCSR pairs represent the locations of imbalances of chromatin accessibility between alleles and are ideal to reveal …

The Colby Echo (2013-05-08), Colby College

The Colby Echo

Published by the students of Colby College since 1877, The Colby Echo is the weekly, editorially independent student-run newspaper of Colby College in Waterville, Maine. Published monthly, 1877-1886; semi-monthly, 1886-1897; and weekly, during the academic year, 1898-present.

Characterization Of System Xc -, A Cystine-Glutamate Amino Acid Transporter, As An Effector Of Interleukin-1Β-Mediated Injury And Neuroprotection, Nicole A. Jackman

Doctoral Dissertations

Interleukin-1β (IL-1β) is a key mediator in the inflammatory response essential to cellular defense against pathogen invasion and to the repair of tissue damage. While some studies suggest that IL-1β signaling is harmful to the injured central nervous system, others report neuroprotective effects that appear to be context-dependent. Previously, the Hewett laboratory demonstrated IL-1β-mediated increases in the cystine-glutamate transporter, system x_c-, as a novel contributor to inflammatory hypoxic neuronal injury. The focus of this doctoral research was to elucidate the specific cell type(s) in mixed cortical cultures that respond to IL-1β by enhancing the activity of system x …

Building A Community-Based Food System: Green Economic Development In Central Illinois, Tim Glaza

Capstone Projects – Politics and Government

It has been a challenge for many community-based, small to medium scale, fruit and vegetable farmers to meet the high demand of locally sourced food. Community-based producers have been mostly using farm stands, farmers’ markets and community-supported agriculture (CSAs) to meet this demand. Although there has been tremendous growth in the number of farmers’ markets and CSA subscriptions, a gap remains for institutional buyers demanding community-based food. Regional food hubs may be the critical piece needed for filling this gap. The Edible Economy Project in partnership with Heartland Community College is working on a regional food hub network project in …

The Role Of Family Organization In Family Health History Communication About Cancer, Vivian M. Rodríguez

Theses and Dissertations

Family health history (FHH) has been recognized as an important tool in cancer prevention and health promotion. To date, literature on FHH discussions about cancer have largely focused on patient-physician communication or the dissemination of cancer-specific genetic tests results within the family. Fewer studies have sought to identify family factors that may promote FHH discussions, yet this type of information could be used to identify families needing support in having these conversations. Thus, the present study examined relations between family organization (cohesion and flexibility), communication openness, and FHH communication about cancer within a diverse group of women recruited from an …

From The Museum To The Parking Lot: A Scrutiny Of The Everyday, Julie D. Cronan

Undergraduate Theses—Unrestricted

My work is inspired by my investigations of overlooked places in the cities that I live, experiences that I seek to translate through drawing and printmaking.This paper follows the evolution of my BFA thesis work at Washington University in St. Louis, from my initial sketches in museums to my more recent, sustained investigations of parking lots and strip malls. Key issues raised are the history of knowledge, especially in relation to science and museums, and whether it is possible to know a place through firsthand observation. The paper focuses specifically on the subjectivity of drawing and ways that the drawing …

Can Long-Range Pcr Be Used To Amplify Genetically Divergent Mitochondrial Genomes For Comparative Phylogenetics? A Case Study Within Spiders (Arthropoda: Araneae), Andrew G. Briscoe, Sara Goodacre, Susan E. Masta, Martin I. Taylor, Miquel A. Arnedo, David Penny, John Kenny, Simon Creer

Biology Faculty Publications and Presentations

The development of second generation sequencing technology has resulted in the rapid production of large volumes of sequence data for relatively little cost, thereby substantially increasing the quantity of data available for phylogenetic studies. Despite these technological advances, assembling longer sequences, such as that of entire mitochondrial genomes, has not been straightforward. Existing studies have been limited to using only incomplete or nominally intra-specific datasets resulting in a bottleneck between mitogenome amplification and downstream high-throughput sequencing. Here we assess the effectiveness of a wide range of targeted long-range PCR strategies, encapsulating single and dual fragment primer design approaches to provide …

Graduate Student Research Meeting Minutes, Wku Graduate Council

Graduate School

Meeting minutes.

Weak Scattering Of Scalar And Electromagnetic Random Fields, Zhisong Tong

Open Access Dissertations

This dissertation encompasses several studies relating to the theory of weak potential scattering of scalar and electromagnetic random, wide-sense statistically stationary fields from various types of deterministic or random linear media. The proposed theory is largely based on the first Born approximation for potential scattering and on the angular spectrum representation of fields. The main focus of the scalar counterpart of the theory is made on calculation of the second-order statistics of scattered light fields in cases when the scattering medium consists of several types of discrete particles with deterministic or random potentials. It is shown that the knowledge of …

A Scaffold Model Of The Outer Retina, Kristina R. Russano

Open Access Theses

Patients with diseases that lead to the loss of the cells of their outer retina, such as AMD and retinitis pigmentosa, suffer from an irreversible loss of vision. Since the body has no mechanism to restore the structure or function of these cells, could photoreceptors be transplanted to restore function? Several groups have attempted transplantations, but were faced with the challenges of low retention and inefficient delivery. Also, a limitation of photoreceptor research is their poor survival in culture due to their dependence on RPE. We purpose that a two-layered biodegradable polymer scaffold that serves as support and a permeable …

Vbp15, A Glucocorticoid Analogue, Is Effective At Reducing Allergic Lung Inflammation In Mice, Jesse M. Damsker, Blythe C. Dillingham, Mary C. Rose, Molly A. Balsley, Christopher R. Heier, Alan M. Watson, Erik J. Stemmy, Rosalyn A. Jurjus, Tony Huynh, Kathleen Tatem, Kitipong Uaesoontrachoon, Dana M. Berry, Angela S. Benton, Robert J. Freishtat, Eric P. Hoffman, John M. Mccall, Heather Gordish-Dressman, Stephanie L. Constant, Erica K.M. Reeves, Kanneboyina Nagaraju

Pharmacology and Physiology Faculty Publications

Asthma is a chronic inflammatory condition of the lower respiratory tract associated with airway hyperreactivity and mucus obstruction in which a majority of cases are due to an allergic response to environmental allergens. Glucocorticoids such as prednisone have been standard treatment for many inflammatory diseases for the past 60 years. However, despite their effectiveness, long-term treatment is often limited by adverse side effects believed to be caused by glucocorticoid receptor-mediated gene transcription. This has led to the pursuit of compounds that retain the anti-inflammatory properties yet lack the adverse side effects associated with traditional glucocorticoids. We have developed a novel …

Ndls Update 05/07/2013, Notre Dame Law School

NDLS Update

NDLS Update is a weekly email newsletter of news, events, and opportunities for Law School faculty and staff.

May 7, 2013, University Of Mississippi. Faculty Senate

Meeting Minutes

Minutes for the May 7, 2013 meeting of the University of Mississippi Faculty Senate.

Spartan Daily May, 7 2013, San Jose State University, School Of Journalism And Mass Communications

Spartan Daily (School of Journalism and Mass Communications)

Volume 140, Issue 50

An Improved Clearing And Mounting Solution To Replace Chloral Hydrate In Microscopic Applications1, Thomas S. Villani, Adolfina R. Koroch, James E. Simon

Publications and Research

• Premise of the study: This study presents Visikol™, a new proprietary formulation that can be used as an efficient replacement for chloral hydrate as a clearing agent for microscopic examination. In the United States, chloral hydrate is regulated and therefore difficult to acquire. • Methods and Results: Fresh and dry samples of the following plants: ginger (Zingiber officinale), maté (Ilex paraguariensis), lime basil (Ocimum americanum), oregano (Origanum vulgare), and mouse-ear cress (Arabidopsis thaliana), were cleared using Visikol or chloral hydrate solution and compared using a light microscope. • Conclusions: This new method can be used successfully to clear specimens, …

Method Of Inhibiting Ab-Type Bacterial Toxins And Treatment For Associated Diseases, Michael Taylor, Kenneth Teter

UCF Patents

Disclosed herein are compounds useful in inhibiting the action of AB-type bacterial toxins such as cholera, traveler's diarrhea, enterohemorrhagic diarrhea caused by E. coli O157:H7 and pertussis or whooping cough. Also included in the invention is the use of these compounds in treatment of diseases associated with those toxins.

A Celebration Of Faculty Scholarship - 2013, Dean's Office, College Of The Holy Cross

Annual Bibliography of Faculty Publications

A listing of the scholarly and creative work produced by the faculty of the College of the Holy Cross. It includes articles, book chapters, productions, exhibitions, grants and reviews reported for the 2012-2013 academic year.

"Mich Beschäftigen Vor Allem Dinge, Die Sich In Meiner Umgebung Abspielen": Konstruktionen Des Privaten Und Des Öffentlichen In Der Literatur Der Brd Und Der Ddr, Christina E. Getaz

German and Russian Studies Honors Projects

Common knowledge assumes that capitalism and socialism structured life differently with regards to what was public and private. This paper critically investigates this notion, focusing not on property, but on the realm of ideas and personal experiences. With a basis in historical and theoretical notions of the public and private spheres as conceptualized by Hannah Arendt, I analyze critical works by Jurek Becker and Heinrich Böll written in the two German states in the 1970s. Focusing on the ways in which these works depict an entanglement of the public and the private, I show how the East- and West-German works …

2013 May 7 - Medical Student Education Committee Minutes, Medical Student Education Committee, East Tennessee State University

Medical Student Education Committee Minutes

No abstract provided.

Medill Showalter Says Nc State Work Provides Roadmap To Alter Plants, Ohio University College Of Arts & Sciences

All Forum Articles

No abstract provided.

Dating Human Cultural Capacity Using Phylogenetic Principles, J. Lind, P. Lindenfors, S. Ghirlanda, K. Lidén, M. Enquist

Publications and Research

Humans have genetically based unique abilities making complex culture possible; an assemblage of traits which we term “cultural capacity”. The age of this capacity has for long been subject to controversy. We apply phylogenetic principles to date this capacity, integrating evidence from archaeology, genetics, paleoanthropology, and linguistics. We show that cultural capacity is older than the first split in the modern human lineage, and at least 170,000 years old, based on data on hyoid bone morphology, FOXP2 alleles, agreement between genetic and language trees, fire use, burials, and the early appearance of tools comparable to those of modern hunter-gatherers. We …

The Advocate, May 7, 2013, Minnesota State University Moorhead

The Advocate

No abstract provided.

Worm Imaging Enlightens Lou Gehrig's Research, Greg Williams

Browse All News

No abstract provided.

Gardner-Webb Football Player Embraces His Calling In The Nursing Profession, Office Of University Communications

Gardner-Webb NewsCenter Archive

He’s a tough guy on the football field. As a GWU Runnin’ Bulldog, he plays offensive center. Snapping the football from the line of scrimmage and safely delivering it to the quarterback is a primary responsibility.

Lead Uptake In Diverse Plant Families: A Study Applying X-Ray Absorption Near Edge Spectroscopy, Gudrun L. Bovenkamp, Alexander Prange, Wolfgang Schumacher, Kyungmin Ham, Aaron P. Smith, Josef Hormes

Faculty Publications

The chemical environment of lead in roots and leaves of plants from four different plant families and a lichen from a former lead mining site in the Eifel Mountains in Germany was determined by Pb L3-edge XANES measurements using solid reference compounds and also aqueous solutions of different ionic strength simulating the plant environment. Pb2+ ions in the plants were found to have two major coordinations, one with nine oxygen atoms in the first coordination shell similar to outer-sphere complexation and a second coordination with just three oxygen atoms similar to inner-sphere complexation. This can be interpreted assuming that lead …