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Cal Poly Magazine, Summer 2013

Cal Poly Magazine

No abstract provided.

Biob 171n.01: Principles Of Biological Diversity Lab, Gregory Peters

University of Montana Course Syllabi

No abstract provided.

Biob 170n.01: Principles Of Biological Diversity, Gregory Peters

University of Montana Course Syllabi

No abstract provided.

B Cell Response And Hemagglutinin Stalk-Reactive Antibody Production In Different Age Cohorts Following 2009 H1n1 Influenza Virus Vaccination, Mark Y. Sangster, Jane Baer, Felix W. Santiago, Theresa T. Fitzgerald, Natalia A. Ilyushina

Dartmouth Scholarship

The 2009 pandemic H1N1 (pH1N1) influenza virus carried a swine-origin hemagglutinin (HA) that was closely related to the HAs of pre-1947 H1N1 viruses but highly divergent from the HAs of recently circulating H1N1 strains. Consequently, prior ex- posure to pH1N1-like viruses was mostly limited to individuals over the age of about 60 years. We related age and associated dif- ferences in immune history to the B cell response to an inactivated monovalent pH1N1 vaccine given intramuscularly to subjects in three age cohorts: 18 to 32 years, 60 to 69 years, and >70 years. The day 0 pH1N1-specific hemagglutination inhibition (HAI) …

Uv Radiation And The Skin, John A. D'Orazio, Stuart G. Jarrett, Alexandra Amaro-Ortiz, Timothy Scott

Toxicology and Cancer Biology Faculty Publications

UV radiation (UV) is classified as a "complete carcinogen" because it is both a mutagen and a non-specific damaging agent and has properties of both a tumor initiator and a tumor promoter. In environmental abundance, UV is the most important modifiable risk factor for skin cancer and many other environmentally-influenced skin disorders. However, UV also benefits human health by mediating natural synthesis of vitamin D and endorphins in the skin, therefore UV has complex and mixed effects on human health. Nonetheless, excessive exposure to UV carries profound health risks, including atrophy, pigmentary changes, wrinkling and malignancy. UV is epidemiologically and …

Bioactive Lipids And Cationic Antimicrobial Peptides As New Potential Regulators For Trafficking Of Bone Marrow-Derived Stem Cells In Patients With Acute Myocardial Infarction, Anush V. Karapetyan, Yuri M. Klyachkin, Samy Selim, Manjula Sunkara, Khaled M. Ziada, Donald A. Cohen, Ewa K. Zuba-Surma, Janina Ratajczak, Susan S. Smyth, Mariusz Z. Ratajczak, Andrew J. Morris, Ahmed Abdel-Latif

Internal Medicine Faculty Publications

Acute myocardial infarction (AMI) triggers mobilization of stem cells from bone marrow (BM) into peripheral blood (PB). Based on our observation that the bioactive sphingophospholipids, sphingosine-1 phosphate (S1P), and ceramide-1 phosphate (C1P) regulate trafficking of hematopoietic stem cells (HSCs), we explored whether they also direct trafficking of non-hematopoietic stem cells (non-HSCs). We detected a 3–6-fold increase in circulating CD34+, CD133+, and CXCR4+ lineage-negative (Lin−)/CD45− cells that are enriched in non-HSCs [including endothelial progenitors (EPCs) and very small embryonic-like stem cells (VSELs)] in PB from AMI patients (P<0.05 vs. controls). Concurrently, we measured a 3-fold increase in S1P and C1P levels in plasma from AMI patients. At the same time, plasma obtained at hospital admission and 6 h after AMI strongly chemoattracted human BM-derived CD34+/Lin− and CXCR4+/Lin− cells in Transwell chemotaxis assays. This effect of plasma was blunted after depletion of S1P level by charcoal stripping and was further inhibited by the specific S1P1 receptor antagonist such as W146 and VPC23019. We also noted that the expression of S1P receptor 1 (S1P1), which is dominant in naïve BM, is reduced after the exposure to S1P at concentrations similar to the plasma S1P levels in patients with AMI, thus influencing the role of S1P in homing to the injured myocardium. Therefore, we examined mechanisms, other than bioactive lipids, that may contribute to the homing of BM non-HSCs to the infarcted myocardium. Hypoxic cardiac tissue increases the expression of cathelicidin and β-2 defensin, which could explain why PB cells isolated from patients with AMI migrated more efficiently to a low, yet physiological, gradient of stromal-derived factor-1 in Transwell migration assays. Together, these observations suggest that while elevated S1P and C1P levels early in the course of AMI may trigger mobilization of non-HSCs into PB, cathelicidin and β-2 defensin could play an important role in their homing to damaged myocardium.

Gender And Ses Effects On Multidimensional Self-Concept Development During Adolescence, Patricia Meredith Orr

Doctoral

The present study constitutes an integral part of the Dublin Child Development Study, a longitudinal study which began in 1986 and which has had eight waves of data collection since its inception. Using data collected during two of these waves, multidimensional self-concept status of 72 participants (F = 40, M = 32) was examined at 10 (T1) and 17 (T2) years of age. Longitudinal changes in multidimensional self-concept between T1 and T2 were also examined. The Shavelson Hubner and Stanton Structural Model (1976) was used as the theoretical basis for this research; this model emphasises the multidimensionality of the self-concept …

Insectography: Insects In The World Of Fiction, Erin Bauer

Department of Entomology: Dissertations, Theses, and Student Research

This is a comprehensive list of fictional works (films/TV episodes, video games, children’s books, and novels) originally published or produced in the United States between 1950 and 2012 about or including insects, spiders, related arthropods (many times anthropomorphized or mutated) and/or entomologists as main characters and playing a major role in the plot. Although the list encompasses both entertaining and educational works, it does not include textbooks or other non-fictional works or “fictionalized” accounts of real processes. For example, books such as Hope for the Flowers, where the story is told from the perspective of the main character, a …

Decomposition And Nutrient Release Of Different Cover Crops In Organic Farm Systems, Jianru Shi

School of Natural Resources: Dissertations, Theses, and Student Research

Cover crops act as green manure adding organic matter to agricultural-soils. For legume green manures to be an effective nitrogen (N) source for organic farming systems, their N release must be in synchrony with crop N demand. The objectives of this study were 1) determine the decomposition rates of three common cover crops (white clover, (Trifolium repens,L ) red clover (Trifolium pratense L.) and soybean (Glycine max L) in order to determine when most N was released and its synchrony with subsequent corn crop uptake; 2) we focused on the effect of cover crops on soil …

Electrospun Plant Protein Scaffolds With Fibers Oriented Randomly And Evenly In Three-Dimensions For Soft Tissue Engineering Applications, Shaobo Cai

Department of Textiles, Merchandising and Fashion Design: Dissertations, Theses, and Student Research

In this work, electrospinnable and water stable soyprotein was extracted by using a reducing agent in mild alkaline condition, and novel 3D zein and 3D pure soyprotein electrospun scaffolds with three-dimensionally and randomly oriented fibers and large interconnected pores were successfully fabricated by reducing surface resistivity of materials. This unique structure is different from most electrospun scaffolds with fibers oriented mainly in one direction. The structure of novel 3D scaffolds could more closely mimic the 3D randomly oriented fibrous architectures in many native extracellular matrixes (ECM). Confocal laser scanning microscope shows that instead of becoming flattened cells when cultured in …

Mexico's Missed Opportunities To Protect Irregular Women Transmigrants: Applying A Gender Lens To Migration Law Reform, Alyson L. Dimmitt Gnam

Washington International Law Journal

Mexico is a transit country for hundreds of thousands of migrants traveling north. Due to economic liberalization, women increasingly migrate in search of employment opportunities, a phenomenon called the “feminization of migration.” As women migrate, they face high risks of sexual and gender-based violence, including sexual assault, rape, kidnapping, and trafficking. During transit, the impunity of organized criminal groups and corrupt state officials facilitate rampant abuse of women. Mexico’s former migration policy exacerbated women’s vulnerability to abuse by criminal organizations by pushing women into dangerous illicit migration channels. In response to the abuse of transmigrants, Mexico passed a sweeping migration …

Were All Devonian Seeds Cupulate? A Reinvestigation Of Pseudosporogonites Hallei, Xenotheca Bertrandii, And Aglosperma Spp., Cyrille Prestianni, Jason Hilton, Walter L. Cressler

Earth & Space Sciences Faculty Publications

No abstract provided.

Pheromone Exposure Influences Preoptic Arginine Vasotocin Gene Expression And Inhibits Social Approach Behavior In Response To Rivals But Not Potential Mates, Lisa A. Mangiamele, Alex D.T. Keeney, Erin N. D'Agostino, Richmond R. Thompson

Biological Sciences: Faculty Publications

The nonapeptides arginine vasotocin (AVT) and vasopressin mediate a variety of social behaviors in vertebrates. However, the effects of these peptides on behavior can vary considerably both between and within species. AVT, in particular, stimulates aggressive and courtship responses typical of dominant males in several species, although it can also inhibit social interactions in some cases. Such differential effects may depend upon AVT influences within brain circuits that differ among species or between males that adopt alternative reproductive phenotypes and/or upon the differential activation of those circuits in different social contexts. However, to date, very little is known about how …

Voluven For Treatment Of Hypovolemia In Anesthetic Practice, Irina Son

Theses and Dissertations

Many patients undergoing surgical procedures require intravenous fluid replacement with a colloid volume expander. It is a well-known fact that the choice of intravenous fluid therapy may impact postoperative outcomes in both positive and 4 negative ways (Topcu et al., 2012). In 2007, the Food and Drug Administration (FDA) approved the use of 6% Hetastarch 130/0.4, also known as Voluven, to treat hypovolemia. This product was introduced to the anesthesia practice as a promising volume expander, carrying minimal side effects such as renal failure or coagulopathy. However, over the past few years multiple studies have been performed to test Voluven's …

Soundings 2013-06-01, N/A N/A

Soundings

No abstract provided.

Stent Graft Blush, Vanessa Barrett, Thomas Matzinger, Mike Morelli

Mechanical Engineering

In order to better understand Type IV endoleaks (“blush”), the Cal Poly Senior Project team GraftTest has developed a blush evaluation system able to mimic several of the aorta’s environmental parameters. Capable of both pressure and flow rate control, this bench top system accommodates Medtronic’s wide range of abdominal and thoracic aortic stent grafts. Having researched endoleaks, stent grafts, and numerous other factors affecting stent graft blush, a system was developed with the aim of assessing and comparing the performance of Medtronic’s stent grafts with regard to blush. Furthermore, this system has been designed to provide reliable and repeatable results …

Stability Of Diluted Adenosine Solutions In Polyolefin Infusion Bags, Elise Almagambetova, David Hutchinson, Danielle M. Blais, Fang Zhao

Doctoral External Publications

Background

Intravenous or intracoronary adenosine is used in the cardiac catherization lab to achieve maximal coronary blood flow and determine fractional flow reserve.

Objective

To determine the stability of adenosine 10 and 50 µg/mL in either 0.9% sodium chloride injection or 5% dextrose injection in polyolefin infusion bags stored at 2 temperatures, refrigeration (2°C-8°C) or controlled room temperature (20°C-25°C).

Methods

Adenosine 10 µg/mL and 50 µg/mL solutions were prepared in 50 mL polyolefin infusion bags containing 0.9% sodium chloride injection or 5% dextrose injection and stored at controlled room temperature or under refrigeration. Each combination of concentration, diluent, and storage …

Design And Installation Of A Mobile Agriculture Chemical Mixing Station, Jason Mendes

BioResource and Agricultural Engineering

This senior project encompassed the design and fabrication of a Mobile Agricultural Chemical Mixing System. The design parameters were provided by Mark McKean and McKean Farms from Riverdale, Ca. The project’s objective was to provide safer working conditions for operators by improving their working environment while increasing mixing efficiency. This mobile system will provide storage for materials, as well as, a diversified mixing system at the site of applications.

The major components of the project include two chemical mixers to mix materials, a 2-inch suction pump to supply water from non-powered sources, and a flatbed trailer to encompass the unit …

Cardiac Condition Detection Using Artificial Neural Networks, Sean Franklin, Joseph Wallcave

Electrical Engineering

No abstract provided.

Functional Annotation, Transcriptional Characterization And Enzymatic Contributions Of Essential Amino Acid Biosynthesis-Related Genes, Teresa J. Clark

Masters Theses

In plants, essential amino acid biosynthesis predominantly or exclusively occurs in the plastid. The plastid in the heterokont alga Nannochloropsis oceanica is surrounded by four membranes, which add great complexity to intracellular trafficking and communication. N. oceanica genes in essential amino acid biosynthesis were functionally annotated. The biosynthesis pathways resemble the pathways in Arabidopsis thaliana, but the gene content seems to be simpler in N. oceanica.

In addition, two A. thaliana mutants with loss-of-function mutations in the aspartate kinase-homoserine dehydrogenase 2 (AK-HSDH2) gene were characterized. These ak-hsdh2 mutants demonstrate unexpected accumulation of aspartate-derived amino acids (ADAAs), particularly threonine, in …

Math Department Newsletter, 2013, University Of Dayton. Department Of Mathematics

Department of Mathematics Newsletters

No abstract provided.

The Languages Of Amazonia, Patience Epps, Andrés Pablo Salanova

Tipití: Journal of the Society for the Anthropology of Lowland South America

No abstract provided.

Developing Models Of Disease Transmission: Insights From Ecological Studies Of Insects And Their Baculoviruses, Bret D. Elderd

Faculty Publications

No abstract provided.

Rna Interference-Mediated Depletion Of N-Ethylmaleimide Sensitive Fusion Protein And Synaptosomal Associated Protein Of 25 Kda Results In The Inhibition Of Blood Feeding Of The Gulf Coast Tick, Amblyomma Maculatum, Rebecca Browning, Shahid Karim

Faculty Publications

The signalling pathways in tick salivary glands that control ‘sialo‐secretome’ secretion at the tick−host interface remain elusive; however, this complex process is essential for successful feeding and manipulation of the host haemostatic response. Exocytosis of the sialo‐secretome in the salivary glands requires a core of soluble N‐ethylmaleimide‐sensitive fusion (NSF) attachment proteins (SNAPs) and receptor proteins (SNAREs). SNAREs have been identified as the key components in regulating the sialo‐secretome in the salivary gland cells. In this study, we utilized RNA interference to investigate the functional role of two Amblyomma maculatum SNARE complex proteins, AmNSF and AmSNAP‐25, in the tick salivary glands …

A Robust Estimate For The Bifurcating Autoregressive Model With Application To Cell Lineage Data, Tamer M. E. Elbayoumi

Dissertations

The bifurcating autoregressive model (BAR) is commonly used to model binary tree data. One application for this model relates to cell lineage data in biology. The purpose of studying the cell lineage process is to know whether the observed correlations between related cells are due to similarities in the environmental, inherited effects, or a combination of both of them. Because outliers in this kind of data are quite common, the need for a robust estimation procedure is necessary. A weighted L1 (WL1) estimate for estimating the parameters of the BAR model is considered. When the weights are constant, the estimate …

Tetrathiomolybdate-Associated Copper Depletion Decreases Circulating Endothelial Progenitor Cells In Women With Breast Cancer At High Risk Of Relapse, S. Jain, J. Cohen, M. M. Ward, N. Kornhauser, E. Chuang, T. Cigler, A. Moore, D. Donovan, C. Lam, M. V. Cobham, S. Schneider, Sandra M. Hurtado Rua, S. Benkert, C. Mathijsen Greenwood, R. Zelkowitz, J. D. Warren, M. E. Lane, V. Mittal, S. Rafii, L. T. Vahdat

Mathematics and Statistics Faculty Publications

Background: Bone marrow-derived endothelial progenitor cells (EPCs) are critical for metastatic progression. This study explores the effect of tetrathiomolybdate (TM), an anti-angiogenic copper chelator, on EPCs in patients at high risk for breast cancer recurrence. Patients and methods: This phase 2 study enrolled breast cancer patients with stage 3 and stage 4 without evidence of disease (NED), and stage 2 if triple-negative. TM 100 mg orally was administered to maintain ceruloplasmin <17 mg/dl for 2 years or until relapse. The primary end point was change in EPCs. Results: Forty patients (28 stage 2/3, 12 stage 4 NED) were enrolled. Seventy-five percent patients achieved the copper depletion target by 1 month. Ninety-one percent of triple-negative patients copper-depleted compared with 41% luminal subtypes. In copper-depleted patients only, there was a significant reduction in EPCs/ml by 27 (P = 0.04). Six patients relapsed while on study, of which only one patient had EPCs maintained below baseline. The 10-month relapse-free survival was 85.0% (95% CI 74.6%–96.8%). Only grade 3/4 toxicity was hematologic: neutropenia (3.1% of cycles), febrile neutropenia (0.2%), and anemia (0.2%). Conclusions: TM is safe and appears to maintain EPCs below baseline in copper-depleted patients. TM may promote tumor dormancy and ultimately prevent relapse.

Testing Subspecies Limits In Monardella Villosa, Taylor Mclaughlin Crow

Master's Theses

Several subspecies of Monardella villosa (California Coyote Mint) have been distinguished on the basis of leaf thickness, shape, and trichome characteristics, yet many intermediates are known. We investigated morphological differences in natural populations of two subspecies (M. v. subsp. villosa and M. v. subsp. franciscana) in the Scott Creek watershed, north of Davenport, in Santa Cruz County, CA. Monardella villosa subsp. franciscana grows in coastal scrub in gulches and ocean terraces whereas subsp. villosa grows at more protected inland sites. Morphological difference between subspecies may be adaptations to these different habitats. I grew plants in a common garden at Cal …

Dairy Manure Flushwater Treatment By Packed-Bed Anaerobic Digesters, Neal Cary Adler

Master's Theses

Wastewater treatment performance of three pilot-scale packed-bed anaerobic digesters with walnut shell medium was researched for treating dairy freestall barn flushwater. Reciprocation mixing was evaluated as a means to lessen channelization in the media bed and to improve biogas production and organic matter removal at ambient temperatures. Reciprocation has been used in biological nitrogen removal systems to introduce air into the system to repeatedly oxygenate nitrifying biofilm along with mixing (Behrends et al. 2003), but the anaerobic systems benefit from mixing. Two tanks were used in each system, where one was full and one was empty at any given time. …

Nutrient Removal Using Microalgae In Wastewater-Fed High Rate Ponds, Matthew N. Rodrigues

Master's Theses

This thesis discusses the mechanisms associated with the removal of organic matter, nitrogen and phosphorus in wastewater-fed high rate algae ponds (HRAP) designed to operate as triplicates. Research was conducted at the San Luis Obispo Water Reclamation Facility (SLOWRF) as a pilot-scale study of nine 30-square meter ponds one foot in depth. During period of study, triplicates were operated at hydraulic retention times (HRT) of 4, 3 or 2-days. Main objectives for the study were to determine minimum HRTs required to achieve secondary and tertiary treatment. Experimental conditions such as CO₂ supplementation, nighttime aeration and operation of ponds in …

Establishment And Validation Of A Novel Fucci (Fluorescent Ubiquitination-Based Cell Cycle Indicator) In An In Vitro Chronic Myeloid Leukemia Model, Jane Marie Isquith

Master's Theses

The deregulation of the cell cycle is important in the leukemic transformation and in the continued discovery of more efficient targeted therapies. After elucidation of the role of the RNA editor ADAR1 in the malignant reprogramming of Chronic Myeloid Leukemia (CML) progenitors to leukemia stem cells (LSCs) during the chronic phase to blast crisis stages of the disease, current research seeks to uncover the mechanism of action of ADAR1 in this process. To study the potential role ADAR1 plays in the changes to cell cycle during this disease progression, a novel Fucci (fluorescent ubiquitination-based cell cycle indicator) system was introduced …