Digital Commons Network^™

Postconcussive Symptoms, Ptsd, And Disease Burden In U.S. Oef And Oif Veterans: A Mediational Model, Joah Landon Williams

Electronic Theses and Dissertations Archive

The most common psychological and cognitive sequelae associated with deployments to Afghanistan (OEF) and Iraq (OIF) are mild traumatic brain injury (mTBI) and posttraumatic stress disorder (PTSD). Each of these injuries places Veterans at risk for long-term health problems. One long-term problem associated with mTBI is the development of persistent, postconcussive symptoms, although relations between postconcussive symptoms, PTSD, and objective and subjective health indices have not been empirically explored. This study examined whether PTSD mediates relations between persistent, postconcussive symptoms related to mTBI and two indices of medical disease burden: the number of disease categories positive for a diagnosis, or …

A Broad Look At Open Access From The Biomed Central Perspective, Bob Schatz

American Association for the Advancement of Science Pacific Conference

• Open access publishing is providing a meaningful option to researchers and libraries outside of the traditional subscription model of publishing.
• With the entrance of traditional publishers and government/funder mandates, one can assume that the model is sustainable and here to stay.
• Because of funding issues, OA may work better in some disciplines than in others.
• OA will continue to grow and morph into new forms.
• OA is one of many viable responses to the challenges we face in disseminating the results of research.

Nnb News, Local Seafood Markets Offer Different Atmosphere, Lower Prices, June 16, 2013, Tyler Killette, Chelsea Tatham

Neighborhood News Bureau

A spotlight on Mastry's Tackle seafood shop in St. Petersburg, Florida.

Nnb News, Local Farmers Market Give Midtown More Fresh Produce Options, June 16, 2013, Jaclyn Cayavec, Meaghan Habuda

Neighborhood News Bureau

A spotlight on two farmers markets in St. Petersburg, Florida: the Gulfport Tuesday Morning Fresh Market and the Saturday Morning Market.

Arthur A. Dugoni School Of Dentistry 119th Commencement, University Of The Pacific

Commencement Programs

No abstract provided.

Levels And Chemodynamics Of Pesticides In Soil And Sediments At Kilombero Sugarcane Plantations And Environs, Morogoro Region, Tanzania, James T. Zacharia

Journal of Humanities and Social Sciences

metabolites in soil and sediment samples from Kilombero sugarcane plantations and environs, Morogoro Region, Tanzania. The samples were screened for 56 different pesticide residues and their metabolites using GC-ECD and GC-NPD and the results were confirmed by GC-MS. A total of 31 soil samples and 19 sediment samples were analysed in this study and a total of 16 different pesticides/metabolites were detected in soil samples; and 12 different pesticides/metabolites were detected in sediment samples. o,p’-DDT was shown to have the highest concentration of all the detected pesticides/metabolites in soil samples with a mean concentration of 21.04µg/kg d.w whereas the highest …

A Prairie Rendezvous, Vol. 15, No. 2 (Summer 2013), Grand Prairie Friends

Newsletters

No abstract provided.

The Murray Ledger And Times, June 15, 2013, The Murray Ledger And Times

The Murray Ledger & Times

No abstract provided.

Purification And Characterization Of An Extracellular Chitinase From Rhizopus Oryzae, Wei-Ming Chen, Ching-San Chen, Shann-Tzong Jiang

Journal of Marine Science and Technology–Taiwan

A chitinase with about 50 kDa of molecular mass from Rhizopus oryzae was purified to electrophoretical homogeneity after DEAE Sepharose and Sephacryl S-200 chromatographs, with specific activity of 165.2 U/mg, 19.7% recovery and 4.3-fold of purification. It had optimal pH and temperature at 5.5-6.0 and 60°C, respectively, and was stable at pH 5.0-8.5 and below 50°C. Ca2+, Sr2+, Ba2+, Mn2+, Co2+ and β-Me enhanced chitinase activity by 10-48% ,whereas Hg2+ and SDS strongly inhibited enzyme activity. This enzyme was identified that might be with both chitinolytic and amylolytic activity. According to the result of LC-MS/MS analysis, 4 identified amino acid …

Eastern Washington University Commencement Program, 2013, Eastern Washington University

Commencement Programs

Program for Eastern Washington University's spring commencement in 2013

Design, Fabrication, And Analysis Of A 3u Cubesat Platform, Todd Chun-Van Osdol, Charles Dorsey, Jake Hedlund, Thomas Hoye, Owen Jacobs, Kadja Klarreich-Giglio, Elliott Martin, Michael Ruiz, Michael Schlesselmann, Zachary Singh

Mechanical Engineering Senior Theses

The goal of this project is to develop a 3U CubeSat nanosatellite bus capable of short duration technology verification experiments while providing compelling handson student education opportunities. This 3U CubeSat platform has been prototyped by the design team in order to demonstrate its capabilities. This spacecraft, the Unspecified Payload Active Attitude Control Nanosatellite (UPAACN), is designed to perform a 3-6 month technology verification experiment for a NASA test component and an SCU single-axis attitude control system (AACS) in Low Earth Orbit (LEO). This project builds upon the flight heritage of SCU's Robotic Systems Laboratory (RSL) and improves its satellite development …

Caloric Restriction Augments Radiation Efficacy In Breast Cancer., Anthony D Saleh, Brittany A Simone, Juan Palazzo, Jason E Savage, Yuri Sano, Tu Dan, Lianjin Jin, Colin E Champ, Shuping Zhao, Meng Lim, Frederica Sotgia, Kevin Camphausen, Richard G Pestell, James B Mitchell, Michael P Lisanti, Nicole L Simone

Kimmel Cancer Center Faculty Papers

Dietary modification such as caloric restriction (CR) has been shown to decrease tumor initiation and progression. We sought to determine if nutrient restriction could be used as a novel therapeutic intervention to enhance cytotoxic therapies such as radiation (IR) and alter the molecular profile of triple-negative breast cancer (TNBC), which displays a poor prognosis. In two murine models of TNBC, significant tumor regression is noted with IR or diet modification, and a greater regression is observed combining diet modification with IR. Two methods of diet modification were compared, and it was found that a daily 30% reduction in total calories …

Study Of The Thermodynamics Of Chromium(Iii) And Chromium(Vi) Binding To Iron(Ii/Iii)Oxide Or Magnetite Or Ferrite And Magnanese(Ii) Iron (Iii) Oxide Or Jacobsite Or Manganese Ferrite Nanoparticles, Steven Luther, Nathan Brogfeld, Jisoo Kim, Jason Parsons

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Chemistry)

Removal of chromium(III) or (VI) from aqueous solution was achieved using Fe3O4, and MnFe2O4 nanomaterials. The nanomaterials were synthesized using a precipitation method and characterized using XRD. The size of the nanomaterials was determined to be 22.4±0.9 nm (Fe3O4) and 15.5±0.5 nm (MnFe2O4). The optimal binding pH for chromium(III) and chromium(VI) were pH 6 and pH 3. Isotherm studies were performed, under light and dark conditions, to determine the capacity of the nanomaterials. The capacities for the light studies with MnFe2O4 and Fe3O4 were determined to be 7.189 and 10.63 mg/g, respectively, for chromium(III). The capacities for the light studies …

Role Of Mitochondria In Hiv Infection, Gaofei Lu

Open Access Dissertations

HIV-1 hijacks our cellular machinery to complete its life cycle. A better understanding of the interactions between cellular proteins and viral components will certainly lead to the discovery of new ways to inhibit viral replication. My primary research goals are directed toward understanding the basis of molecular interactions between viral and host factors (proteins or organelles) during infection of human cells. In my thesis, the role of mitochondria in the early stage of HIV infection has been evaluated by using a VSV-G pseudotyped HIV-based lentiviral vector. I have observed that cells lacking mitochondrial DNA (ρ0 cells), isolated from an established …

Conference Program, Engineering Conferences International

BioEnergy IV: Innovations in Biomass Conversion for Heat, Power, Fuels and Chemicals

No abstract provided.

A New Versatile Primer Set Targeting A Short Fragment Of The Mitochondrial Coi Region For Metabarcoding Metazoan Diversity: Application For Characterizing Coral Reef Fish Gut Contents, Matthieu Leray, Joy Y. Yang, Christopher P. Meyer, Suzanne C. Mills, Natalia Agudelo, Vincent Ranwez, Joel T. Boehm, Ryuji J. Machida

Publications and Research

Introduction The PCR-based analysis of homologous genes has become one of the most powerful approaches for species detection and identification, particularly with the recent availability of Next Generation Sequencing platforms (NGS) making it possible to identify species composition from a broad range of environmental samples. Identifying species from these samples relies on the ability to match sequences with reference barcodes for taxonomic identification. Unfortunately, most studies of environmental samples have targeted ribosomal markers, despite the fact that the mitochondrial Cytochrome c Oxidase subunit I gene (COI) is by far the most widely available sequence region in public reference libraries. This …

Amperometric Detection Of Bioamines In Cancer Borealis Using Microchip Capillary Electrophoresis Integrated With Micellar Chromatography, Ajay Fernandez, Jason Howard, Christina Shuh

Bioengineering Senior Theses

In order to study neuromodulation in Cancer borealis, commonly known as the Jonah crab, we designed a microchip capillary electrophoretic (CE) device with micellar electrokinetic chromatography (MEKC) to separate bioamines and detect their concentrations using amperometric detection (AD). The analysis will be used to characterize the bioamines: dopamine, octopamine, tyramine, serotonin, norepinephrine, and gammaaminobutyric acid (GABA). The device measures the concentration of bioamines in microdialysate samples taken directly from the pericardial cavity of the crab. The research on Jonah crabs will be applied to enhance our understanding of the crab neurology. Since the structure and in some instances, the function …

Copper(Ii) Triflate-Mediated Synthesis Of 1,3,5-Triarylpyrazoles In [Bmim][Pf6] Ionic Liquid And Evaluation Of Their Anticancer Activities, V. Kameshwara Rao, Rakesh Tiwari, Bhupender S. Chhikara, Amir Nasrolahi Shirazi, Keykavous Parang, Anil Kumar

Biomedical and Pharmaceutical Sciences Faculty Publications

A simple, efficient, and environmentally friendly protocol for the synthesis of 1,3,5-triarylpyrazole in [bimm][PF₆] ionic liquid mediated by Cu(OTf)₂ is described. The reaction protocol gave 1,3,5-triarylpyrazoles in good to high yields (71-82%) via a one-pot addition–cyclocondensation between hydrazines and chalcones, and oxidative aromatization without requirement for an additional oxidizing reagent. The catalyst can be reused up to four cycles without much loss in the catalytic activity. The pyrazoles (4a-o) and pyrazolines (3a-n) were evaluated for antiproliferative activity in SK-OV-3, HT-29, and HeLa human cancer cells lines. Among all compounds, 3b inhibited cell proliferation …

When To Start Antiretroviral Therapy: The Need For An Evidence Base During Early Hiv Infection, James D. Lundgren, Abdel G. Babiker, Fred M. Gordin, Alvaro H. Borges, James D. Neaton

Epidemiology Faculty Publications

Background

Strategies for use of antiretroviral therapy (ART) have traditionally focused on providing treatment to persons who stand to benefit immediately from initiating the therapy. There is global consensus that any HIV+ person with CD4 counts less than 350 cells/μl should initiate ART. However, it remains controversial whether ART is indicated in asymptomatic HIV-infected persons with CD4 counts above 350 cells/μl, or whether it is more advisable to defer initiation until the CD4 count has dropped to 350 cells/μl. The question of when the best time is to initiate ART during early HIV infection has always been vigorously debated. The …

A Preliminary Report Of Percutaneous Craniofacial Osteoplasty In A Rat Calvarium, William J. Parkes, Md, Jewel Greywoode, Md, Brian J. O'Hara, Md, Ryan N. Heffelfinger, Md, Howard Krein, Md, Phd

Department of Otolaryngology - Head and Neck Surgery Presentations and Grand Rounds

Objective: To evaluate the potential for injectable, permanent bone augmentation by assessing the biocompatibility and bioactivity of subperiosteal hydroxylapatite (Radiesse) deposition in a rat model.

Methods: Fourteen adult Sprague Dawley rats were injected in the parietal skull with hydroxylapatite (n=10) or a carrier gel control (n=4), using a subperiosteal injection technique on the right and a subcutaneous injection technique on the left. At 1, 3, and 6 months, 3 rats (1 negative control, 2 variables) were sacrificed. At 12 months, the remaining 5 rats were sacrificed. After each harvest, the calvaria were examined under both light and polarized microscopy.

Results: …

The Effect Of Anterior Knee Pain On Serum Cartilage Oligomeric Matrix Protein And Muscular Cocontraction During Running, Scott T. Woodland

Theses and Dissertations

Knee pain can alter lower-extremity neuromechanics and often results in functional disability. The relationship between lower-extremity neuromechanical alterations, due to anterior knee pain, and articular cartilage condition is unclear. The purpose of this study was to determine the independent effect of anterior knee pain during running on articular cartilage condition, as reflected by serum cartilage oligomeric matrix protein concentrations and muscle cocontraction duration. Seven men and five women completed a 30-min run in three different sessions: control (no infusion), sham (isotonic saline infusion), and pain (hypertonic saline infusion). Saline was infused into the right infrapatellar fat pad for the duration …

Brain Structures Associated With Temperament And Social Behavior In Rhesus Monkeys: An Mri Study, Andrew C. Chaffin

Theses and Dissertations

Temperament is thought to be the foundation for normative personality and subsequent behaviors later in life. To assess the relationship of early temperament with variation in structural brain development, this study examined rhesus macaque mother-infant behavior, and then three years later, used MRI to assess neurostructural differences. Individual differences in mother-infant interactions and emotionality were then linked to brain differences. Extensive behavioral data obtained over the first year of life under both resting and stressful conditions was used to assess the quality of mother-infant interactions and emotionality. The MRI focused on brain volume in areas thought to be related to …

Someone’S In The Kitchen, Where’S Dinah? Gendered Dimensions Of The Professional Culinary World, Stephanie Konkol

College of Education Theses and Dissertations

Traditionally cooking is considered to be women’s work, yet the vast majority of professional chefs, particularly in the upper echelons of restaurant work, are men. These curious gendered patterns stimulated interest in delving more deeply into the gendered nature of restaurant work. Existing research on this topic has concentrated on the front of the house (dining room) but has not addressed the gendered nature of the male-dominated back of the house (kitchen). This study looks at the gendered nature of the professional culinary kitchen (back of the house), how gender relations are constructed through culinary education and in the professional …

The Intellectual And Curricular Spaces Of Knowledge Studies, Jay H. Bernstein

Publications and Research

The words “knowledge” and “information” are sometimes used interchangeably, but the connection between them is complex and problematic. Knowledge is a mental product gained from engaging with information. All educational subjects, scholarly disciplines, occupations, and activities produce knowledge as well as information. Because libraries encompass potentially all subjects, professional vision in librarianship would benefit from an examination of knowledge that transcends the methods and topical concerns of individual disciplines. An interdisciplinary (or transdisciplinary) framework in which to view knowledge was pioneered in the post-Sputnik age by Fritz Machlup and Michael Polanyi. Their insights have stimulated scholars to develop research, publications, …

Phylogeny Of Acridoidea (Orthoptera: Caelifera) Based On Complete Mitochondrial Genome Data: Testing Partition Strategies, James Leavitt, Dr. Michael Whiting

Journal of Undergraduate Research

The orthopteran superfamily Acridoidea (Orthoptera, Caelifera), which includes grasshoppers and their nearest relatives, is the largest of the orthopteran superfamilies and provides an excellent model for studying the evolution of mitochondrial genomes. Ultimately I was able to reconstruct a phylogeny for Caelifera based on entire mitochondrial genomes (mtgenomes) in order to (1) evaluate the relative effectiveness of various data partitioning strategies within a Maximum Likelihood (ML) framework; (2) evaluate the conservation of anti-codons across the group and elucidate patterns among the start and stop codons of the mitochondrial protein coding genes (MPCGs); and (3) specifically test the monophyly of Acridoidea …

The Effect Of Male Dominance On Female Mate Choice In Poecilia Gillii, Michael Mcentire, Dr. Jerald Johnson

Journal of Undergraduate Research

Female mate choice (intersexual selection) and male dominance interactions (intrasexual selection) can each play important roles in sexual selection. These two mechanisms tend to be discussed in isolation. The goal of this study is to explore the interaction between these two forms of sexual selection. To test this idea, we focused on the livebearing tropical fish system Poecilia gillii (See Figure 1). We grouped males into similarly sized pairs and observed them for a week to determine which male was dominant. These pairs were then presented to females in mate choice trials to ascertain female preference. Our data appears to …

Does Secretory Phospholipase A Ii Induce Apoptosis In Cells That Have Been Infected By A Virus?, Stephanie Melchor, Dr. John Bell

Journal of Undergraduate Research

Well, to be perfectly honest, this project has been fraught with disaster since the beginning. We obtained 400 microliters of the immunosuppressive strain of Minute Virus of Mice (MVMi) at 1 x 10⁹ pfu/mL from Dr. David J. Pintel at the University of Missouri-Columbia. MVMi was the only virus we could find that would infect our cell line (S49 mouse T-lymphoma cells. Because S49 cells are suspension cells and not adherent, they do not form plaques when infected with a virus, which makes it more difficult to quantify how many cells have been successfully infected with the virus. Instead, …

Effects Of Antiviral Drug Compounds In Blocking Influenza Virus M2 Channels In Xenopus Laevis Ooctyes, Timothy Mcclain, David Busath

Journal of Undergraduate Research

The purpose of this project was to research and test various drug compounds to prevent influenza virus infection. A specific target of my research is M2 protein channels that aid in the uncoating of the viral coat. Recent mutations have rendered formerly effective drugs almost completely useless. With recent outbreaks and mutations of the virus, the demand for antiviral drugs has never been greater. The goal of the project was to test several different compounds that had been selected based on unique characteristics, designed to adjust to the mutation, and prevent further infection.

The Spondyloepiphyseal Dysplasia Congenita Homozygous Mutant: A Model For The Mechanism Of Osteoarthritis, David Macdonald, Dr. Robert Seegmiller

Journal of Undergraduate Research

Articular cartilage is a hard-wearing and specialized form of hyaline cartilage which allows for an almost frictionless surface during joint movement (Ofek et al. 2008). These unique characteristics are primarily due to chondrocyte secretions, and the composition and properties of the extracellular matrix (ECM) (Buckwalter and Mankin 1998). Chondrocytes play a crucial role in cartilage durability because of their extracellular secretions (Knudson and Knudson 2001). Being immotile, chondrocytes depend greatly upon the equilibrium of the ECM for protection, integrity, and regulation (Aszodi et al. 2003). The ECM is composed primarily of type II collagen, glycoproteins, water and aggrecan proteoglycan (Hollander …

Protection Of Dietary Polyphenols Against Oral Cancer, Yijian Ding, Hua Yao, Yanan Yao, Leonard Yenwong Fai, Zhuo Zhang

Toxicology and Cancer Biology Faculty Publications

Oral cancer represents a health burden worldwide with approximate 275,000 new cases diagnosed annually. Its poor prognosis is due to local tumor invasion and frequent lymph node metastasis. Better understanding and development of novel treatments and chemo-preventive approaches for the preventive and therapeutic intervention of this type of cancer are necessary. Recent development of dietary polyphenols as cancer preventives and therapeutic agents is of great interest due to their antioxidant and anti-carcinogenic activities. Polyphenols may inhibit carcinogenesis in the stage of initiation, promotion, or progression. In particular, dietary polyphenols decrease incidence of carcinomas and exert protection against oral cancer by …